LOCUS       NM_001370658            9964 bp    mRNA    linear   PRI 13-OCT-2024
DEFINITION  Homo sapiens biotinidase (BTD), transcript variant 3, mRNA.
ACCESSION   NM_001370658 NM_000060
VERSION     NM_001370658.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Sharma,R., Kucera,C.R., Nery,C.R., Lacbawan,F.L., Salazar,D. and
            Tanpaiboon,P.
  TITLE     Biotinidase biochemical and molecular analyses: Experience at a
            large reference laboratory
  JOURNAL   Pediatr Int 66 (1), e15726 (2024)
   PUBMED   38299772
  REMARK    GeneRIF: Biotinidase biochemical and molecular analyses: Experience
            at a large reference laboratory.
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   Moatter,T., Ahmed,S., Majid,H., Jafri,L., Bilal,M., Najumuddin,
            Faisal and Khan,A.H.
  TITLE     Sequence variants in the BTD underlying biotinidase deficiency in
            families of Pakistani origin
  JOURNAL   J Gene Med 26 (1), e3597 (2024)
   PUBMED   37751899
  REMARK    GeneRIF: Sequence variants in the BTD underlying biotinidase
            deficiency in families of Pakistani origin.
REFERENCE   3  (bases 1 to 9964)
  AUTHORS   Erdol,S., Kocak,T.A. and Bilgin,H.
  TITLE     Evaluation of 700 patients referred with a preliminary diagnosis of
            biotinidase deficiency by the national newborn metabolic screening
            program: a single-center experience
  JOURNAL   J Pediatr Endocrinol Metab 36 (6), 555-560 (2023)
   PUBMED   37119528
  REMARK    GeneRIF: Evaluation of 700 patients referred with a preliminary
            diagnosis of biotinidase deficiency by the national newborn
            metabolic screening program: a single-center experience.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9964)
  AUTHORS   Tankeu,A.T., Van Winckel,G., Elmers,J., Jaccard,E.,
            Superti-Furga,A., Wolf,B. and Tran,C.
  TITLE     Biotinidase deficiency: What have we learned in forty years?
  JOURNAL   Mol Genet Metab 138 (4), 107560 (2023)
   PUBMED   37027963
  REMARK    GeneRIF: Biotinidase deficiency: What have we learned in forty
            years?
REFERENCE   5  (bases 1 to 9964)
  AUTHORS   Hymes,J., Stanley,C.M. and Wolf,B.
  TITLE     Mutations in BTD causing biotinidase deficiency
  JOURNAL   Hum Mutat 18 (5), 375-381 (2001)
   PUBMED   11668630
  REMARK    GeneRIF: review of mutations causing biotinidase deficiency
            Review article
REFERENCE   6  (bases 1 to 9964)
  AUTHORS   Cole,H., Reynolds,T.R., Lockyer,J.M., Buck,G.A., Denson,T.,
            Spence,J.E., Hymes,J. and Wolf,B.
  TITLE     Human serum biotinidase. cDNA cloning, sequence, and
            characterization
  JOURNAL   J Biol Chem 269 (9), 6566-6570 (1994)
   PUBMED   7509806
REFERENCE   7  (bases 1 to 9964)
  AUTHORS   Wolf,B.
  TITLE     Biotinidase Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301497
REFERENCE   8  (bases 1 to 9964)
  AUTHORS   Klein,C., Lohmann,K., Marras,C. and Munchau,A.
  TITLE     Hereditary Dystonia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301334
REFERENCE   9  (bases 1 to 9964)
  AUTHORS   Oizumi,J. and Hayakawa,K.
  TITLE     Enkephalin hydrolysis by human serum biotinidase
  JOURNAL   Biochim Biophys Acta 1074 (3), 433-438 (1991)
   PUBMED   1679665
REFERENCE   10 (bases 1 to 9964)
  AUTHORS   Wolf,B., Grier,R.E., Secor McVoy,J.R. and Heard,G.S.
  TITLE     Biotinidase deficiency: a novel vitamin recycling defect
  JOURNAL   J Inherit Metab Dis 8 Suppl 1, 53-58 (1985)
   PUBMED   3930841
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC027129.6 and AC090950.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 21, 2019 this sequence version replaced NM_000060.4.
            
            Summary: The protein encoded by this gene functions to recycle
            protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine),
            a normal product of carboxylase degradation, resulting in
            regeneration of free biotin. The encoded protein has also been
            shown to have biotinyl transferase activity. Mutations in this gene
            are associated with biotinidase deficiency. Multiple transcript
            variants encoding different isoforms have been described. [provided
            by RefSeq, Aug 2013].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.247939.1,
                                           SRR1803611.210300.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000643237.3/
                                                ENSP00000495254.2
            RefSeq Select criteria           :: based on manual assertion,
                                                conservation, expression,
                                                longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               AC027129.6         45210-45359         c
            151-415             AC027129.6         11416-11680         c
            416-565             AC027129.6         5047-5196           c
            566-3350            AC027129.6         4-2788              c
            3351-9964           AC090950.3         40536-47149
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.1"
     gene            1..9964
                     /gene="BTD"
                     /note="biotinidase"
                     /db_xref="GeneID:686"
                     /db_xref="HGNC:HGNC:1122"
                     /db_xref="MIM:609019"
     exon            1..150
                     /gene="BTD"
                     /inference="alignment:Splign:2.1.0"
     exon            151..415
                     /gene="BTD"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1738
                     /gene="BTD"
                     /EC_number="3.5.1.12"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 3; biotinase; biocytinase"
                     /codon_start=1
                     /product="biotinidase isoform 1 precursor"
                     /protein_id="NP_001357587.1"
                     /db_xref="CCDS:CCDS63565.1"
                     /db_xref="GeneID:686"
                     /db_xref="HGNC:HGNC:1122"
                     /db_xref="MIM:609019"
                     /translation="MSGARSKLALFLCGCYVVALGAHTGEESVADHHEAEYYVAAVYE
                     HPSILSLNPLALISRQEALELMNQNLDIYEQQVMTAAQKDVQIIVFPEDGIHGFNFTR
                     TSIYPFLDFMPSPQVVRWNPCLEPHRFNDTEVLQRLSCMAIRGDMFLVANLGTKEPCH
                     SSDPRCPKDGRYQFNTNVVFSNNGTLVDRYRKHNLYFEAAFDVPLKVDLITFDTPFAG
                     RFGIFTCFDILFFDPAIRVLRDYKVKHVVYPTAWMNQLPLLAAIEIQKAFAVAFGINV
                     LAANVHHPVLGMTGSGIHTPLESFWYHDMENPKSHLIIAQVAKNPVGLIGAENATGET
                     DPSHSKFLKILSGDPYCEKDAQEVHCDEATKWNVNAPPTFHSEMMYDNFTLVPVWGKE
                     GYLHVCSNGLCCYLLYERPTLSKELYALGVFDGLHTVHGTYYIQVCALVRCGGLGFDT
                     CGQEITEATGIFEFHLWGNFSTSYIFPLFLTSGMTLEVPDQLGWENDHYFLRKSRLSS
                     GLVTAALYGRLYERD"
     exon            416..565
                     /gene="BTD"
                     /inference="alignment:Splign:2.1.0"
     exon            566..9964
                     /gene="BTD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2038..2043
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTD"
                     /note="hexamer: AATAAA"
     polyA_site      2068
                     /gene="BTD"
                     /note="major polyA site"
     regulatory      3865..3870
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTD"
                     /note="hexamer: AATAAA"
     polyA_site      3890
                     /gene="BTD"
     regulatory      9938..9943
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTD"
                     /note="hexamer: ATTAAA"
     regulatory      9942..9947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTD"
                     /note="hexamer: AAGAAA"
     polyA_site      9961
                     /gene="BTD"
     polyA_site      9964
                     /gene="BTD"
ORIGIN      
        1 aggagattgc tgcctatgca aagcaggtaa gaagccgaac tctgaggcct ctcgccattg
       61 tctccgagtc ggccagctgg agcgttttcg gggctgtaaa gggagaatgg cgcatgcgca
      121 tattcagggc ggaaggcgcg ctaagagcag atttgtggtc tgcattatgt ctggagccag
      181 aagtaagctt gctcttttcc tctgcggctg ttacgtggtt gccctgggag cccacaccgg
      241 ggaggagagc gtggctgacc atcacgaggc tgaatattat gtggctgccg tgtatgagca
      301 tccatccatc ctgagtctga accctctggc tctcatcagc cgccaagagg ccttggagct
      361 catgaaccag aaccttgaca tctatgaaca gcaagtgatg actgcagccc aaaaggatgt
      421 acagattata gtgtttccag aagatggcat tcatggattc aactttacaa gaacatccat
      481 ttatccattt ttggacttca tgccgtctcc ccaggtggtc aggtggaacc catgcctgga
      541 gcctcaccgc ttcaatgaca cagaggtgct ccagcgcctg agttgtatgg ccatcagggg
      601 agatatgttc ttggtggcca atcttgggac aaaggagcct tgtcatagca gtgacccaag
      661 gtgcccaaaa gatgggagat accagttcaa cacaaatgtc gtgttcagca ataatggaac
      721 ccttgttgac cgctaccgta aacacaacct ctactttgag gcagcattcg atgttcctct
      781 taaagtggat ctcatcacct ttgatacccc ctttgctggc aggtttggca tcttcacatg
      841 ctttgatata ttgttctttg accctgccat cagagtcctc agagactaca aggtgaagca
      901 tgttgtgtac ccaactgcct ggatgaacca gctcccactc ttggcagcaa ttgagattca
      961 gaaagctttt gctgttgcct ttggcatcaa cgttctggca gctaatgtcc accacccagt
     1021 tctggggatg acaggaagtg gcatacacac ccctctggag tccttttggt accatgacat
     1081 ggaaaatccc aaaagtcacc ttataattgc ccaggtggcc aaaaatccag tgggtctcat
     1141 tggtgcagag aatgcaacag gtgaaacgga cccatcccat agtaagtttt taaaaatttt
     1201 gtcaggcgat ccgtactgtg agaaggatgc tcaggaagtc cactgtgatg aggccaccaa
     1261 gtggaacgtg aatgctcctc ccacatttca ctctgagatg atgtatgaca atttcaccct
     1321 ggtccctgtc tggggaaagg aaggctatct ccacgtctgt tccaatggcc tctgctgtta
     1381 tttactttac gagaggccca ccttatccaa agagctgtat gccctggggg tctttgatgg
     1441 gcttcacaca gtacatggca cttactacat ccaagtgtgt gccctggtca ggtgtggggg
     1501 tcttggcttc gacacctgtg gacaggaaat cacagaggcc acggggatat ttgagtttca
     1561 cctgtggggc aacttcagta cttcctatat ctttcctttg tttctgacct cagggatgac
     1621 cctagaagtc cctgaccagc ttggctggga gaatgaccac tatttcctga ggaaaagtag
     1681 gctgtcctct gggctggtga cggcggctct ctatgggcgc ttgtatgaga gggactagga
     1741 aaagtgtgtg gtctgtgggg cggactctgg ccatcatgtt gacagccttg cacttccaca
     1801 ggctacaagc cctgggacca tctttctgcc ttaagggcag gagcccactt ctgtggcacc
     1861 agattccacc ctgggaactg tggaaaaagt aggagaggca gattccctca gtgtcttcct
     1921 cttaaacctc aatcatcgag acattagggg gtattttctg ttcacattta tctttttcaa
     1981 gccacatctt cctctaacaa atctctcagt atgcgattgg tctcaagcta aaacaaaaat
     2041 aaatgtcagt ttatatttta cacatccaca aagcagtggc ttggggtttt tttttttttt
     2101 tttatcttgt tgatcaagtg acacccagga catgtaaata tttcataagc cttaaacatt
     2161 tcctgaggta agaaacaagc tctcaaagca aaagctcaat tagaaatggc ccttgtgggg
     2221 aaccttccca ttctggtcga ccagaactct agccagatga aatggcaatg ctagcgccac
     2281 cagcaacgtc agaaacgtag accttaaagc ggcttttaaa aatagaaaag aagcgttcct
     2341 cacatctgcc agtaatggaa ttttctgtca gtaaatggaa tgtgtaggca ggacctggaa
     2401 taactggaga gagtgcaacg cttcggggtg aagggcgggt ggggactgga aatgttgaga
     2461 cgggggcagc catgggaagg tatgagtaat agaattcttt ctgtacgaca cagctcatcc
     2521 agggattcca gggaccttaa taaatcacgg tagctttggg caagagttgg gcacgttgcc
     2581 cgactgtgca ggatggattg atgctggtat aatttgatct ggagccctat agaggatctc
     2641 gttgctttgc agcgatgcct ggcaccccat agagataagt tgcagggact gccgtgaagg
     2701 gcatgaaggg tggcccactt tagtcatgca tttattcaat cacccaacag gcacagagca
     2761 ggagcttccc atgttaggaa ttccagatgc aaaattatca gactcaatat ggtgaagaca
     2821 tttttaggcc actgatttct gtgtaatttg tgtccaatat ttcaaagcca tttaaaaaag
     2881 gattttgact gcatgcctag tagctgtttc agatcatttt ttaagtacag aatggataat
     2941 tagtcttcag tgatttgcct cttaaatgtg gccatttagt ttgaactcct ttttagttat
     3001 tttaaacagt atatgcttaa acatcacacg attttataaa gcaagaaaaa tgaaatgatc
     3061 tccctattcc cccacggtgt aggcctccag atccttttct ggcttttagg caggacaatt
     3121 aacaatgtgg attctgcagt cagactgatg gggtttccat gcatggcctc accattaact
     3181 cctcactggg gagaccttgg gcctcggttt tttattcata gaatgcatgt tgaataaacc
     3241 atgtgggaaa aagcagagcc agcaccagcc cctgctgact cactgatccc tcggaaaggt
     3301 ctgggatgag tcaagtcttc agggaataag cagcaaggac ttggaattcc tgagcaagcc
     3361 ttgggttccc cacgccctag gaagtccctc tggaagggga gcaggacctc tcccaccaga
     3421 tgacggagtt cgctttctcg cccagcgcag ctcccaccag gcgtgagctg gcagtccaga
     3481 agcggcccca gatgtggcta cccaccgccc agtctgcttc cctgcctcaa accccaggga
     3541 gacagaaatt acctaaaaat gcatatcatt gccttatgcc aagatgattc aaataagcct
     3601 gagaaagaga ggccagcgct aaatacagac ttggcagaac tacttcaccc agtaaacatc
     3661 taatcagcac ctgcaggatc ctcattctgc ccttctgggc agagcaccac gaactttccc
     3721 tgagttattt tctaccaaca atttagaatg aattgctcgt gaagtagaaa tgaggggaaa
     3781 agcaactaat ggtggtagaa caaagtaatt tttacaatta aaagaagtag ctgccagatg
     3841 acagaacaaa gtagaggacg ggaaaataaa tcattttcag ggaaaaaata tatctatgac
     3901 taatggttgt gattatgttt ttgtcgattg tgatcctgaa actcaggaag caaatggcaa
     3961 tcttgaccac aacatggaga caggaaatca aatgcatttt tggctaaacc agacttgggg
     4021 gaagtggctt ggtttgcgtg gtttctcatg taggtgcagc tgtgcttttc tgtggttttt
     4081 ctcacacggg aagtgagagg cacagtgagc tgtattcaca ctggatttaa aagccctgtt
     4141 cttagagagg aaaaaaccag gcctcctcac agactgccaa gtcccagagc ccaccatgtc
     4201 tgctctcaag acacaggcct gtccagagcc cctgctgagg gaaacccacc caggccactt
     4261 ttccccacag gtggccctgc ttggtacctg tgcccccacc ccccagccat ggtatattga
     4321 accaggagga ggcacccagc ccaaggccag ccaatcagag cactggctag tagcccagga
     4381 ggtgtccctg cacagaagat ctcctcagag aggaccgatg gtgagtcggc cgctcttgat
     4441 tctcaatgaa actcagaacc gctcaagtga aatgaccact caaagaacaa tttctcaaca
     4501 aagtggattt atataggaca aaataggcat aaaagtatga aatgttttgt gcacaaaggc
     4561 ctagatttga gacaagagct gatgtggcct gaagatctgt gagtgggact gcttgggagg
     4621 ccatgttcct tctcaggcca gagaaaggtc ctggtctcct ggcttcagtc tttcattccc
     4681 taacctccct ttgaatgtta gttacctatt cccacataac aaagtgcccc aaaacttaat
     4741 ggcataaaac aagcatggac attgttagtt cacccagttc tgtggggcag gaaagcgggt
     4801 ctctcatgag gctacagtca agatgtcagc cgaggtgaca gtgaagatat caaccaaggc
     4861 tgcagccggt tgaaggcctc actggatttg gagaatatta ttgattaaat tgcgtcttcc
     4921 ccaaaaaaga tacgttgtag tcctaacccc caatagctca gaatgtggcc ttatttggaa
     4981 atagggtctt tgcagatgca atgaagatat aagttaaaat gagatcacac tagagtaact
     5041 gggcccttaa tccaatatga catgtcctta tcagaagagg agaagacaca cacacaggga
     5101 gaagatgacc atgtgacaac aggggcagag atcacaggga tgcctctact agacaaggca
     5161 caccagaaat tgcttgccaa caaacaccag aaattagaag aggcaagaaa caactcccca
     5221 cacacaaccc acccctgcag gtttcagaag aagcatggct ctgccaacac cttgattttg
     5281 gatttccagc ttccacaact atgagacaat aagtttctgt tgtttgaagc cacccagttc
     5341 agggtacttt gatatggcag acctaggaaa caaatacaga ggatcacttg caaaatggcc
     5401 cattcacaca gctggcacgt tggtgctggc tgctagcaaa aggtctcagc tcctcaccac
     5461 ttgggtcttt ccatagggat agctgagtgt cctcatgatg tggcccctgg cttcccctga
     5521 agcaaatgat ccaagagagc acaggtggag ccatgatgtc ttttattaac tagtctcaga
     5581 agtcacactg catcatttgg caccatctta ttgagttaca caggtcagcc ctgctcccta
     5641 tggaaagaga cagagggtgt tagatgccca gaggtgaacc tcattgggta ccatcctgca
     5701 ggtgggctgc cactctgctc aatccaaatc acagctctcc cgtttctcac ctcccaacag
     5761 ttgctgttcc tcaggccaaa gttcacattc tgcacagcct cagctccgcc tgacctctga
     5821 gctcacatga tggcccttac tgtgaacctg ggtttctggt ccagccatag ctacttcttt
     5881 aagctcctcc agtgtgctga gcacagtctg gcccctgtac tgttgtctgc ttgaaatggc
     5941 gtcttctgat gaacactcat ccatccttca aggtctactc tctcatcaca gcttgtgact
     6001 cttccacttt ttgaactggt gtttcccatt cccagttcac agagcccttt ctcattgaac
     6061 tatttatctg agttccctct gccggaacat gagccatgcc tagagtagcc acctagtagt
     6121 gagtgacagc tctgtgctgg atgcacataa atggtctccc ttaactgcca tgagccctaa
     6181 agaaggtttg ctacagctat tttacagatg gggaaaactg acagagagat attaatgaat
     6241 tgcccacatg caaatatgtg ctgagtcttg gatttgcatc tttatcgtga ctccacggag
     6301 acccaccctc taagaccaga gccagtgtcc tattcatctt ttgtctctgc agcgttcagc
     6361 atggcactgt cttggcttac aaaatctgct ctatgcttgc tgactgctga atgaatgaat
     6421 gaatgaatag gtagtcacaa agaatgttta gaatgtttct cagacaggct gagaaaaaac
     6481 acaacgaaac attatttccg tttggaaagt ttttttattt ttgtgttcag tactgaagta
     6541 aaacaaaaat ctgaataaca gctgcaccgt taaaaatgaa attaccaata tatgaactct
     6601 aggcatcatg catatataat tttttgtaga taacttttct tctcattttc cttctcattc
     6661 tcttcatctt tttctttttg tttgagcaaa aaaaaaaaag aatatattaa ctcgtaactg
     6721 gggagtctaa gaataaattc tcttcagcca ttgctgaatc taggggctca aatggtgtct
     6781 ctggacacag tatcacactc tactctgcac tcttctttat cattttctcc cacagacaga
     6841 ctctcgtgta gttgcaacaa tggccatgac ttctaggctt catctgcccc tctcagcagc
     6901 tctagagaag aagctcctct ttttacacag tccctaatta aagtctcaga gatgaacaac
     6961 ctgggctctt gcttagggca cgtgcccatc ctgaatcaat gatcggccag agagctgtga
     7021 tattcttttg tttttttgag atggagtctt gctctgtcgc ccgggcagga gtgcaatggc
     7081 atgatctcgg ctcactgcaa gctctgcctc ccgagttcaa gcgattctcc tgcctcagcc
     7141 ttccgagtag ctgggactac aggcacgtgc caccacgccc agctaatttt ttgtattttt
     7201 atagagacag ggtttcacca tgttagccag gatggcctcg atctcctgac ctcatgatct
     7261 gcctgcctca gcctcccaaa gtgctgggat tacaggcgtg agccaccgta cccggccagg
     7321 gctgtgatat tctgatcagc cagcactgag tcacatggcc actattaggg tgagggatga
     7381 taggcttagc tctgacctga aattcatgga ttgaggggtg gggactgttt cccaggggaa
     7441 cactgggaca gcgttaccag gggaagggga gtgggatata gttgggccaa aacagtaaat
     7501 gtccacgagg cttttttcct ctgagcagaa atttcactgt gatgccagtc aggtattgct
     7561 gccgtaaggg ccatagcagg agggagactg caaatcttga ttgaggagat cttaagaaca
     7621 gagtgattta cacagctgtg ggcaggatgt aggggaaccc agaaaggata atgcactacc
     7681 cagcggccag cgacagcagg agctatgagc accccaggcc tgaaggtgtg aagggctgaa
     7741 atggttacca gaagcaggag agggaagctt atggagagag caggctggcg ggagcagggg
     7801 ctgtgagaga gacagccaga ccgtagcggt gtgacaggca gagagccagg cgatccatat
     7861 cctgtctcct ctctcttctc cgctcctgac tcctgctgcg gcctcccact ggctggaccc
     7921 aacaggaaag cagaggataa gagaatctgg ttggtgctat ctagaagtca gtgttgggga
     7981 ggtcacagaa cagaatggag aggggtagag tatgcaggga agataaatgc agccctggca
     8041 tggttcttcc agaacattct gcaagctcat ggaccagttc atttgagaag aaatagagga
     8101 gttaggggag agagaagctt cttaatgaaa ataaccttgg tagctagtct caagattagc
     8161 ccccagtgct ccccacctgc tggtatccgt tgtacaacac ttcccacatt tttctggggt
     8221 cgttttgtgt aaccaataaa atatggcaga tgcgatggca tgttacttct gaaattgggt
     8281 tataaaaggt actgcagacc gggcacggtg gcttacgcct gtaatcccag cactttggga
     8341 ggccaagcct ggcggatcac aaggttagga gttcaagacc agtctggcca atatggtgaa
     8401 accctgtctc tattgaaaat acaaaaatta gccggcatgg tggcacacgc ctgtagtccc
     8461 agctagtcgg gaggctgagg cagaagaatc gcttgaaccc gggaggcgga ggttacaatg
     8521 agctgagata gcaccactgc actccagtct gggcaacaga gcgacactcc gtctccaaaa
     8581 aaaaaagata ctgcagcttc tatcttggtt gctcacttgc tatcttgggt ccctccctct
     8641 ggggaaagcc agttgccatc tcccaaggat ccctatggac agacccattt ggcaaggaac
     8701 agccaccagc tagctgcatg agcgagccat gttggaagtg ggtcctccag ctgcagtcaa
     8761 gcttccagat gaccactgcc ctgggaaacg actgcaacct catgagagac cctgagccgg
     8821 aaccacccca caaagtcact cttgatttcc taagtctcag aaactataaa atataaatgt
     8881 gtgttgtttt aaaatcctag gttttgaggt aatttattat acagcaatag aaaactaata
     8941 caatagtctt gctaccagca ccttaatctc ccacatccaa gttaaaattc atccaacact
     9001 caatctccca gctatcctga ctgctcctat acaggtatct ctcccactat atatatatca
     9061 agtgctatta gtggctatta tttgttgggt tacacaaagc cagaaatcat ctacatctac
     9121 tctcttttct tctggttttg aatagcaaaa tgcaacaggg agatgttctc agatagaggg
     9181 agaacaacaa aaagaagggt ccatgctgaa caaacttgtt tgctttgaaa tcggaagttc
     9241 tagaggaggt attctgacag tggccaggaa aacctcctgc ccgcagaatt ttgtctaatc
     9301 caccccagac agagttatcc aacagcctgg tatggtgcag accaggggct ctcgaaaggt
     9361 gggctccagg cagcagcacc agtagcacct gggaaattgt tggaaatgca aattcttggg
     9421 ctccacccca gatcaactta atcggactct ctgggagtag ggcccatcaa tctgtgttta
     9481 acaagtcctc caggtgattc cgatcctaat caagtgtgag cccactgtct aatgcggtgc
     9541 ataccaaagc aggtgcacgg gtcttaatca tcacctcagt cctctgaaac aggcaagtgt
     9601 gtccatttta cagaagaagg aactaaggcc tggaaggcaa gtccttgcct gagactaaac
     9661 agaatcccag caacatagga cgaggctgga attcccacac ctggactaat gcacagccag
     9721 tgtttcaacg tcgccaaccc agaaatgttt tctctccctg tcatgctaat tttcagccct
     9781 ttctgggtga cttgttcatc atccaaatat attatttttg ttttgtttga ctaaaggcaa
     9841 ttatatttac attttctcta agtactggaa tgtaatggtt gaaattccta ttcagtgatc
     9901 tggaagaact ctaatgttct aaagtgattc tttgataatt aaagaaagat gtaattatgt
     9961 ataa
//
