LOCUS       NM_001002860            8445 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens BTB domain containing 7 (BTBD7), transcript variant 1,
            mRNA.
ACCESSION   NM_001002860
VERSION     NM_001002860.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Liu,Y., Song,J., Gu,J., Xu,S., Wang,X. and Liu,Y.
  TITLE     The Role of BTBD7 in Normal Development and Tumor Progression
  JOURNAL   Technol Cancer Res Treat 22, 15330338231167732 (2023)
   PUBMED   37050886
  REMARK    GeneRIF: The Role of BTBD7 in Normal Development and Tumor
            Progression.
            Review article
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Wang,B., Wang,Y., Wang,X., Gu,J., Wu,W., Wu,H., Wang,Q. and Zhou,D.
  TITLE     Extracellular Vesicles Carrying miR-887-3p Promote Breast Cancer
            Cell Drug Resistance by Targeting BTBD7 and Activating the
            Notch1/Hes1 Signaling Pathway
  JOURNAL   Dis Markers 2022, 5762686 (2022)
   PUBMED   35655918
  REMARK    GeneRIF: Extracellular Vesicles Carrying miR-887-3p Promote Breast
            Cancer Cell Drug Resistance by Targeting BTBD7 and Activating the
            Notch1/Hes1 Signaling Pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8445)
  AUTHORS   Chen,J., Lai,Y.H., Ooi,S., Song,Y., Li,L. and Liu,T.Y.
  TITLE     BTB domain-containing 7 predicts low recurrence and suppresses
            tumor progression by deactivating Notch1 signaling in breast cancer
  JOURNAL   Breast Cancer Res Treat 184 (2), 287-300 (2020)
   PUBMED   32772271
  REMARK    GeneRIF: BTB domain-containing 7 predicts low recurrence and
            suppresses tumor progression by deactivating Notch1 signaling in
            breast cancer.
REFERENCE   4  (bases 1 to 8445)
  AUTHORS   Shu,J., Wang,L., Han,F., Chen,Y., Wang,S. and Luo,F.
  TITLE     BTBD7 Downregulates E-Cadherin and Promotes Epithelial-Mesenchymal
            Transition in Lung Cancer
  JOURNAL   Biomed Res Int 2019, 5937635 (2019)
   PUBMED   31886230
  REMARK    GeneRIF: Studied the role of BTB domain containing 7 (BTBD7) in
            regulation of E-Cadherin in the development of lung cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8445)
  AUTHORS   Grisard,E., Coan,M., Cesaratto,L., Rigo,I., Zandona,L.,
            Paulitti,A., Andreuzzi,E., Rampioni Vinciguerra,G.L., Poletto,E.,
            Del Ben,F., Brisotto,G., Biscontin,E., Turetta,M., Dassi,E.,
            Mirnezami,A., Canzonieri,V., Vecchione,A., Baldassarre,G.,
            Mongiat,M., Spizzo,R. and Nicoloso,M.S.
  TITLE     Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene
            interaction as crucial for colorectal cancer metastasis
  JOURNAL   EBioMedicine 46, 79-93 (2019)
   PUBMED   31303496
  REMARK    GeneRIF: our data indicate that detection of high miR-23b and Btbd7
            levels in the primary tumor of CRC patients can be correlated with
            the development of cancer metastasis and thus patients with these
            biological features should be treated more aggressively and have
            closer follow-ups.
REFERENCE   6  (bases 1 to 8445)
  AUTHORS   Fang,L.Z., Zhang,J.Q., Liu,L., Fu,W.P., Shu,J.K., Feng,J.G. and
            Liang,X.
  TITLE     Silencing of Btbd7 Inhibited Epithelial-Mesenchymal Transition and
            Chemoresistance in CD133+ Lung Carcinoma A549 Cells
  JOURNAL   Oncol Res 25 (5), 819-829 (2017)
   PUBMED   27983936
  REMARK    GeneRIF: Silencing of Btbd7 significantly inhibited the
            self-renewal and EMT process in CD133+ cells. we found that
            downregulation of Btbd7 promoted cell apoptosis and increased the
            sensitivity to paclitaxel in CD133+ and parental cells.
REFERENCE   7  (bases 1 to 8445)
  AUTHORS   Yang,L., Wang,T., Zhang,J., Liu,Z. and Wang,X.
  TITLE     Expression of BTBD7 in primary salivary adenoid cystic carcinoma
            and correlation with Slug and prognosis
  JOURNAL   Cancer Biomark 17 (2), 179-185 (2016)
   PUBMED   27540976
  REMARK    GeneRIF: BTBD7 expression was significantly correlated with Slug
            expression in SACC (P< 0.05). Increased BTBD7 expression was
            significantly associated with the TNM stage, tissue typing, distant
            metastasis and patients' poor clinical outcome.
REFERENCE   8  (bases 1 to 8445)
  AUTHORS   Fan,C., Miao,Y., Zhang,X., Liu,D., Jiang,G., Lin,X., Han,Q.,
            Luan,L., Xu,Z. and Wang,E.
  TITLE     Btbd7 contributes to reduced E-cadherin expression and predicts
            poor prognosis in non-small cell lung cancer
  JOURNAL   BMC Cancer 14, 704 (2014)
   PUBMED   25253020
  REMARK    GeneRIF: Btbd7 contributes to reduced expression of E-cadherin and
            may be a promising cancer marker in non-small cell lung cancer.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8445)
  AUTHORS   Tao,Y.M., Huang,J.L., Zeng,S., Zhang,S., Fan,X.G., Wang,Z.M.,
            Yang,H.X., Yuan,X.H., Wang,P., Wu,F., Luo,J., Zeng,D.Y. and Shen,H.
  TITLE     BTB/POZ domain-containing protein 7: epithelial-mesenchymal
            transition promoter and prognostic biomarker of hepatocellular
            carcinoma
  JOURNAL   Hepatology 57 (6), 2326-2337 (2013)
   PUBMED   23325674
  REMARK    GeneRIF: Cox regression analysis revealed that high BTBD7
            expression integrated with high microvessel density was a powerful
            independent predictive factor of hepatocellular carcinoma clinical
            outcome.
REFERENCE   10 (bases 1 to 8445)
  AUTHORS   Pan,W., Zhang,Q., Xi,Q.S., Gan,R.B. and Li,T.P.
  TITLE     FUP1, a gene associated with hepatocellular carcinoma, stimulates
            NIH3T3 cell proliferation and tumor formation in nude mice
  JOURNAL   Biochem Biophys Res Commun 286 (5), 1033-1038 (2001)
   PUBMED   11527404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC047071.1, AB040958.2, AK074354.1 and AL132838.4.
            
            On Nov 23, 2018 this sequence version replaced NM_001002860.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB040958.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334746.10/ ENSP00000335615.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                BC047071.1         57-151
            96-3907             AB040958.2         81-3892
            3908-4309           AK074354.1         1439-1840
            4310-4831           AB040958.2         4297-4818
            4832-8445           AL132838.4         19872-23485         c
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12"
     gene            1..8445
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /note="BTB domain containing 7"
                     /db_xref="GeneID:55727"
                     /db_xref="HGNC:HGNC:18269"
                     /db_xref="MIM:610386"
     exon            1..217
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            218..405
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             324..3722
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     BTB/POZ domain-containing protein 7; BTB (POZ) domain
                     containing 7"
                     /codon_start=1
                     /product="BTB/POZ domain-containing protein 7 isoform 1"
                     /protein_id="NP_001002860.2"
                     /db_xref="CCDS:CCDS32146.1"
                     /db_xref="GeneID:55727"
                     /db_xref="HGNC:HGNC:18269"
                     /db_xref="MIM:610386"
                     /translation="MGANASNYPHSCSPRVGGNSQAQQTFIGTSSYSQQGYGCESKLY
                     SLDHGHEKPQDKKKRTSGLATLKKKFIKRRKSNRSADHAKQMRELLSGWDVRDVNALV
                     EEYEGTSALKELSLQASLARPEARTLQKDMADLYEYKYCTDVDLIFQETCFPVHRAIL
                     AARCPFFKTLLSSSPEYGAEIIMDINTAGIDMPMFSALLHYLYTGEFGMEDSRFQNVD
                     ILVQLSEEFGTPNSLDVDMRGLFDYMCYYDVVLSFSSDSELVEAFGGNQNCLDEELKA
                     HKAVISARSPFFRNLLQRRIRTGEEITDRTLRTPTRIILDESIIPKKYATVILHCMYT
                     DVVDLSVLHCSPSVGSLSEVQALVAGKPNMTRAEEAMELYHIALFLEFNMLAQGCEDI
                     IAESISLDTLIAILKWSSHPYGSKWVHRQALHFLCEEFSQVMTSDVFYELSKDHLLTA
                     IQSDYLQASEQDILKYLIKWGEHQLMKRIADREPNLLSGTAHSVNKRGVKRRDLDMEE
                     LREILSSLLPFVRIEHILPINSEVLSDAMKRGLISTPPSDMLPTTEGGKSNAWLRQKN
                     AGIYVRPRLFSPYVEEAKSVLDEMMVEQTDLVRLRMVRMSNVPDTLYMVNNAVPQCCH
                     MISHQQISSNQSSPPSVVANEIPVPRLLIMKDMVRRLQELRHTEQVQRAYALNCGEGA
                     TVSYEIQIRVLREFGLADAAAELLQNPHKFFPDERFGDESPLLTMRQPGRCRVNSTPP
                     AETMFTDLDSFVAFHPPLPPPPPPYHPPATPIHNQLKAGWKQRPPSQHPSRSFSYPCN
                     HSLFHSRTAPKAGPPPVYLPSVKAAPPDCTSTAGLGRQTVAAAAATTTSTATAAAAAA
                     SEKQVRTQPVLNDLMPDIAVGVSTLSLKDRRLPELAVDTELSQSVSEAGPGPPQHLSC
                     IPQRHTHTSRKKHTLEQKTDTRENPQEYPDFYDFSNAACRPSTPALSRRTPSPSQGGY
                     FGPDLYSHNKASPSGLKSAYLPGQTSPKKQEEARREYPLSPDGHLHRQKNEPIHLDVV
                     EQPPQRSDFPLAAPENASTGPAHVRGRTAVETDLTFGLTPNRPSLSACSSEAPEERSG
                     RRLADSESLGHGAQRNTDLEREDSISRGRRSPSKPDFLYKKSAL"
     exon            406..1485
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1486..1694
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1770
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1931
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1932..2075
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2265
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2444
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2906
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2907..8445
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8426..8431
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /note="hexamer: AATAAA"
     polyA_site      8445
                     /gene="BTBD7"
                     /gene_synonym="FUP1"
                     /note="major polyA site"
ORIGIN      
        1 ctgctccgtc taggagtcaa aatggcggcg aggggaacct ggagcagtcc cggagcctga
       61 gccactgaca ggagaggaga gggcggcggc ggcggcggtg ggaggaggat ggccgggggt
      121 gctggcgccg gtgcggacgg cggtgctggt ggcggcggcg gcggaggcga cggcagcggt
      181 cccagcggca gcagcagcgg cgggaggagc ctccgggaaa tggatgaaaa gaggctgctg
      241 gatccagggt tgaaggttca taaaggcctc tgggattata ctctgaagaa cccgcagacg
      301 gaatgcctga gtgactgaag aaaatgggtg ctaatgcatc taattatcct cattcatgtt
      361 ccccgagggt agggggaaat tcacaggccc aacagacttt tatagggacc tcatcctatt
      421 ctcagcaagg ctatggttgc gaatcaaagt tgtatagcct tgaccatggc catgagaaac
      481 cacaagacaa aaaaaagaga acctctggtc ttgccaccct caaaaagaag tttattaagc
      541 gtcggaaatc taataggtct gccgatcatg ccaagcagat gcgagaactc ctctctgggt
      601 gggatgttag agatgtcaat gcattagtgg aggaatatga gggaacatca gcattaaagg
      661 agctttctct acaagccagt ttggctagac cagaagcccg gacattgcag aaagatatgg
      721 ctgatcttta tgagtacaag tattgtactg atgtagactt aatatttcaa gaaacttgtt
      781 ttcctgttca tcgtgccatt ttggcagcaa ggtgtccatt ttttaaaaca ctgctttctt
      841 cctcaccaga gtatggggca gagataataa tggacatcaa tacagctggt attgatatgc
      901 ccatgttttc tgctttgtta cactaccttt atacaggaga gtttggaatg gaggactcaa
      961 ggtttcaaaa tgtcgatatc cttgttcagc ttagtgaaga atttggaaca ccaaattccc
     1021 ttgatgtaga tatgcgtgga ctctttgatt acatgtgtta ttatgatgtc gtccttagtt
     1081 tttcttcaga ctctgaactg gttgaagctt ttggtggaaa tcagaactgt ttagatgaag
     1141 agctcaaagc ccacaaggct gttatttctg cacggtcccc attttttcga aatttattac
     1201 aaaggaggat acgaactggt gaagaaatca cagaccgaac tttgaggact cccacaagaa
     1261 ttatattaga tgagtccatt ataccaaaaa aatatgcaac agtgatatta cactgtatgt
     1321 ataccgacgt ggtggacctc tctgttttgc actgtagccc ctctgtgggg agtctcagtg
     1381 aagttcaggc tctcgtcgca gggaagccaa acatgaccag ggcagaagaa gccatggaac
     1441 tttaccacat agcactgttc ttggaattta acatgcttgc acaaggctgt gaggatatca
     1501 ttgctgagag catctcatta gataccttaa ttgccatcct caagtggagt tctcatccat
     1561 atggctctaa atgggtgcac cgacaagctt tacatttcct ctgtgaggaa ttttcccagg
     1621 tcatgacttc ggatgttttt tatgaactca gcaaagacca tctgcttact gctatccagt
     1681 ctgactacct acaggcaagt gaacaagata tccttaaata tctgattaaa tggggagagc
     1741 atcagttgat gaaaagaata gcagatagag agccaaactt actgagtggc actgcccata
     1801 gtgtgaacaa aagaggtgta aaaagacggg acctggacat ggaagagctc agagagatcc
     1861 tttcttctct cttacctttt gtgcgaattg aacacatctt acctataaac agtgaagtct
     1921 taagtgatgc aatgaaaaga ggcttgatta gtactcctcc atcagatatg cttcctacaa
     1981 cagaaggtgg gaagtcaaat gcctggttac ggcaaaaaaa tgctggcatc tatgttcgtc
     2041 ctcgactctt ctctccctat gtggaagaag caaagtcagt gctagatgag atgatggtgg
     2101 aacaaacgga tcttgtgcgc ttgcgaatgg ttagaatgtc caatgtgcca gacacgctct
     2161 acatggtcaa taatgccgtg ccacagtgtt gtcacatgat cagccaccag cagatcagca
     2221 gcaaccagtc aagccctcct tcagttgtag ccaacgaaat tccagttcct cgtctcctca
     2281 ttatgaaaga catggtcaga cgactgcagg aactgcggca cacggagcag gtgcagaggg
     2341 cctatgccct gaactgcggg gaaggcgcca ctgtcagcta tgaaattcag attcgagtgc
     2401 taagagagtt tggtcttgca gatgctgctg cagagctgtt gcagaatcct cacaaattct
     2461 ttcctgatga acgttttggg gatgaaagtc cactcttgac aatgagacag cctgggagat
     2521 gtcgcgtaaa cagtacacct cctgcagaaa ccatgtttac agatctggac tcttttgtgg
     2581 ccttccatcc acccttgccc cctccaccac ctccctacca ccccccagct accccaatcc
     2641 ataaccaact caaagcaggc tggaagcaaa gacctcccag tcagcaccct tcacgttcat
     2701 tttcttatcc ctgtaatcat tcgctgttcc actccagaac agctcctaaa gctggccctc
     2761 ccccagtcta cttgccgagt gtgaaagctg caccgcctga ttgtaccagc actgcaggac
     2821 tgggcagaca gacggtggct gctgctgccg ccaccaccac ctcaacagca acagcagcag
     2881 cagcagcagc atctgagaag caagtgcgaa cacaacctgt gctgaatgat ctgatgccag
     2941 acatcgcggt gggtgtgtcc acactgtcac tcaaggacag gaggcttcca gagcttgctg
     3001 tagacacaga attaagccag tcagtttctg aagcaggacc agggcctccc cagcatctgt
     3061 cgtgtattcc acagagacat acacacactt ctcggaaaaa acacacacta gagcaaaaaa
     3121 cagacaccag agaaaatcca caggaatatc cggatttcta tgacttctca aatgctgctt
     3181 gcagaccttc tactcctgct ctcagcagac gcaccccttc cccttcgcaa ggtggatatt
     3241 ttggtcccga tctgtacagc cacaataagg catcaccaag tggcttaaag tcagcctacc
     3301 tacctggtca gacgtctcct aaaaaacagg aagaagctag gagagaatat ccactttccc
     3361 ctgacgggca tctacacaga caaaagaatg agccgataca cctggatgtc gttgagcaac
     3421 ctccccagcg gtcagacttt cctttggcag ccccagaaaa tgctagtacc ggtccagccc
     3481 atgtcagggg acgaactgca gtagaaactg acttgacttt tgggctgact cctaacagac
     3541 cttcactttc tgcatgtagc tctgaagctc ccgaagagag atccggtaga agactggcag
     3601 acagtgagtc cctgggccat ggagctcaga gaaatacaga tttggaaagg gaagattcaa
     3661 taagcagagg aaggaggtca ccaagcaagc cggacttcct ctacaaaaag tctgccctct
     3721 gagagcaacc tccaagtcgt ctgtgcctga gatgtgaaac atcccatttt atgatgtaac
     3781 ccaacaactt acagaccagt tcatcaatgc cagctgataa ctcggtttca catgattttg
     3841 ttctagtttt tgtgtatata tgtgtttatt agacatggca tgctaagaag gttttgtttt
     3901 ttttttttaa tgttgcatct gtctaatttg tctttgaaca attatgtcgg gaggcatttt
     3961 taagaacaag caggctggca ctttttattt ctctttacaa atgatggaat ttttttgcac
     4021 ttgtacattt attttctctg tattgatttt atatcagtat gttaaacttt attgccacat
     4081 ttaaaggact gtattttcat acttttttgc attttacctt tcatctacca atccatgtgc
     4141 attggattgc acactaagat gtattttctt acgaaatagt tctgtgttta ttttttaatt
     4201 atagaaattc caaagaacag cacatcagaa tgctcctctc ttttcagtaa ttgttttagt
     4261 tcagagatct tcccgctcag cctccataaa tctaatgtca tttttttttt aataagtcct
     4321 aggagctggg cttcattcct ccagcgggag tccagtcagg gtcctgaagt ttgctggttc
     4381 aggaaagaac aggaaagaga aaagccttct gtgcccaggc taaagctgtt acatttccac
     4441 agtgtggatg cagtcagaat ccctgcacat tctggtcata aggattagag tcaccagatg
     4501 aggctctaaa cagatcgcat cccttggaat tagaaagcag tggagaccgg gcgcagtgac
     4561 tcacacctgt gatcggcact tcggaaggcc gaggcgggca gatcactgaa gctcaggagt
     4621 tcaagaccag cctgggcaac atggcgaaac tccatttcta taaaaaatac aaaaaaaaaa
     4681 aaatctagcc aggcgtggtg gtgcgtgcct gtagtcccag ctacttgggg ggctgaggtg
     4741 gcaggatcgc ttgagcctgg gaggttgaga ctgcagtgag ccaagattgc accactgtgc
     4801 tcccacctgg gcaacagtga gaccctgtct caaaaaaaaa aaaaaaggca gtggaaatag
     4861 agaagtaact taaaggtcac actgacagaa tttctggttc tgagcgtcaa ctctccagct
     4921 gtgatggcgg aactgactgc ttgctgcctt taattgtctt gctctctatg ggaaaaaaac
     4981 aaaagggctt cagtctcatg gatgttattt ggtgtttgta aagatacgtt ttcttttaaa
     5041 cgattttcaa aatatgtcta aattttggtg ttgtgtaatg cataatgctt tctcaccaac
     5101 agaaaaaagg aaaaaaattg ttttcagtta aacactacat gtcctacttg aactccactg
     5161 ggacgctggt ttggcattta ctaaaccact gagcacatgg ggtctttcag gatcctctcc
     5221 cttggaaggg caatgtgtga gctaaggact gttttttttc ttactctcca atgtaaatat
     5281 atgagtattt gagtttttaa atcacttcaa cattatttga gcatttactt tgtattttta
     5341 caaacacata aacacacagc cgtagcactt agaagaaaag aggctttcta agcattcctt
     5401 cctctgcagg atatttggga tcctcttttc tatgtatgta tatatataaa aaactatgaa
     5461 tcactcacac tcactcgaaa actatgcagg gctagggagc gtttatgagc taccgggtaa
     5521 atgaattaca tgcactttga agtaaatagg ttttctattg ggtgtcaaca acgctgattt
     5581 ctcccaaaac tatagtttct cttacggtct tttcctcctt taccctgtgg atatacactc
     5641 aggaataatc tgacatttta gaaaagccga acagtagata gtctccaaga tgggaaacat
     5701 gtcgtgtgtc tgcacgcccc ggcaaactgc cgccactcgt tctgctgcct gactggttag
     5761 ctgctgtgcg ggtgcgctcg gtgacactgg tggtggtgca cgctcccgag ctcccagagt
     5821 taacactaat ctgcaggtcg gctgtgcatc gcattcgagg ctcctttttt aaagagaaaa
     5881 gcataggcct ctgttctcat ttgtaagaag tttgactcat aatagaagac ggtgtctttt
     5941 gaagcccagt ctctgtctgt cttcagaaac aaaagtgaat gccgtcatgt tccttgatgt
     6001 gtgtttctgc cacatatgta tgttgacatg ttcatgtact gcgccggtac tgcaagtaag
     6061 actcaacaga cctaatttgg atggggagaa aaactctcat ccctgaccct tccccccccg
     6121 caaatgttag accagagctg ttgatcccac acattcattt ttctgaaaat tcatgaacta
     6181 ttctctcata tccctgaaat ataaaagtaa gtggccacta ttcctaagat tatttagttt
     6241 ataaagtttg cttttaatca tacaatagct tacctggcta aaactttgcg tataaaggga
     6301 aaacttgctt taatctgtgg gattgcggac cgatgcgact tgcagaaggt aaacaggcgt
     6361 gggttttgtg tgcgttaggt taggtgttag tggactttta atgtatcttg tgaagtatgg
     6421 ttactagggt aggttcaaga gctacatgcc tctggctttt gaacccatct attaaatctt
     6481 ttttaatgct gtactaagat aaatgtgaag tctgagtaag gttgctctca gaaaaaaaaa
     6541 aaaagcatat atatatataa aataaaattt aggccatggg aactggcatt ttacttggta
     6601 taggaattta atgagaacca aatcacaaaa ataccagatc tccttttgag atcgtggaca
     6661 atcaggcctt tggacaatca ccgcttccag tgcccgctgt gcgttgaggt tagagaccta
     6721 ccgtttacac gctagagccg agaggacgtt caagtttctt aaagatgctg ttggtctttt
     6781 ttcttatgtt attgttttag ttttgtgatt ttgatgaata attgaagttc agagtcaccc
     6841 gtggctccta aggaaaaaac agcatcatga aaggcaaaaa gtatttgaaa cgtaattgtt
     6901 tcaattttcc acgcccgttc tcacatgccc cctttagctt tatttctggg aacatgtcac
     6961 tagacctcag ctagcctgac accgcaataa cccgttaggc tttgaggctt ttcagtgaat
     7021 tcttgacatt taaatcttct tatagaattc tttatgaagc cttaactaat tcttgttttt
     7081 gcttcctcag aaacttttta ttgcatctgc catccttcat tggatttttg ttttggtttg
     7141 ttgttgccat tctagcaatg agcttagtcc ttgtacatct gcattaaggt gccgatgcag
     7201 aaggcaaatt ggaaacacgc gccagcggca gtggggctca aagccgacct ccatgcatca
     7261 ttctctagag ttgaaaggat tgacatttct cagtaactac ccggcaaaat atatattttc
     7321 ataatgttga atttgccttt taggaaaaca atgtttcatg aaacacttgt aagatactta
     7381 ttttgaaatt aattataagg cattcatatt tttaggactg aattcaaagg gaaatgcttc
     7441 tttgactttt tttcattgtt cggttgactt tcagctttct ggagtgacaa agcagcagtg
     7501 atgggtgcta ttcctggttc tgtggtggtt tactttgtct actaagtgat ttactttcaa
     7561 acctcattac agataatctt actaaaagac ttaacatgca cactgctaaa aggtgtttta
     7621 ctgttttcca ttaacgctgt tgtatgaaca atatttggtg acatgttgtc tttttgtcct
     7681 gtagattgtc atatctgtta tgtagttatc cttttggcaa gtcctttgat ttttttttcc
     7741 tctagaaaat cctcagactg tcaatcttcc atattttgaa tattataatg ctgtggccat
     7801 ttctatagaa agattacttg caagtgtgtg tcttgaaaat aacctcttgt atttgtgtgt
     7861 ttatgtgagt tctgtctaag agatcagata agctctggaa gcttcatcat ctgtacctac
     7921 atgtaactgt atctgggtta catgggcagg cggtaccaag tcctgctctt cgatggggtg
     7981 gctgagagat aaaggttcct gaactctcct tgctgtgtat gcaccacatc tgtacttcag
     8041 agaggtggaa aatcatctac aacactgcgt ttcttgtggt tgtgctgtta ctggtataaa
     8101 gtcaagtgcc ttcaatgcta aaggctcaga aatttttctt aaactgattt catgtcctat
     8161 gcaagtgttt tctacaacct gcataaccag tactttgtaa aacttgttta cgcttcaatt
     8221 gtacatagtg atttttaaag aaatatatag tatttttatt taggtacctc caaacttgaa
     8281 ttcgtctgtg tgaagcattg taaaacgata catttctctt ttgagtacca ttacagttgt
     8341 acagaggttt tcactgcttc tatttttcta ctgttactga taagcatgta acactgactt
     8401 tatcctacat atagttggca tttcaaataa atggcttgta tagaa
//
