LOCUS       NM_003972               8361 bp    mRNA    linear   PRI 03-APR-2024
DEFINITION  Homo sapiens B-TFIID TATA-box binding protein associated factor 1
            (BTAF1), transcript variant 1, mRNA.
ACCESSION   NM_003972
VERSION     NM_003972.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Fugger,K., Chu,W.K., Haahr,P., Kousholt,A.N., Beck,H., Payne,M.J.,
            Hanada,K., Hickson,I.D. and Sorensen,C.S.
  TITLE     FBH1 co-operates with MUS81 in inducing DNA double-strand breaks
            and cell death following replication stress
  JOURNAL   Nat Commun 4, 1423 (2013)
   PUBMED   23361013
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   de Graaf,P., Mousson,F., Geverts,B., Scheer,E., Tora,L.,
            Houtsmuller,A.B. and Timmers,H.T.
  TITLE     Chromatin interaction of TATA-binding protein is dynamically
            regulated in human cells
  JOURNAL   J Cell Sci 123 (Pt 15), 2663-2671 (2010)
   PUBMED   20627952
  REMARK    GeneRIF: Results support a model for a BTAF1-mediated release of
            TBP-NC2 complexes from chromatin.
REFERENCE   3  (bases 1 to 8361)
  AUTHORS   Koutroubas,G., Merika,M. and Thanos,D.
  TITLE     Bypassing the requirements for epigenetic modifications in gene
            transcription by increasing enhancer strength
  JOURNAL   Mol Cell Biol 28 (3), 926-938 (2008)
   PUBMED   18025106
  REMARK    GeneRIF: histone acetylation, although it occurs, is dispensable
            for TFIID and SWI/SNF recruitment by the strong enhancers
REFERENCE   4  (bases 1 to 8361)
  AUTHORS   Grob,P., Cruse,M.J., Inouye,C., Peris,M., Penczek,P.A., Tjian,R.
            and Nogales,E.
  TITLE     Cryo-electron microscopy studies of human TFIID: conformational
            breathing in the integration of gene regulatory cues
  JOURNAL   Structure 14 (3), 511-520 (2006)
   PUBMED   16531235
  REMARK    GeneRIF: We propose that these density rearrangements in the
            structure are a likely reflection of the plasticity of the
            interactions between TFIID and its many partner proteins.
REFERENCE   5  (bases 1 to 8361)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 8361)
  AUTHORS   Wang,Z., Morris,G.F., Rice,A.P., Xiong,W. and Morris,C.B.
  TITLE     Wild-type and transactivation-defective mutants of human
            immunodeficiency virus type 1 Tat protein bind human TATA-binding
            protein in vitro
  JOURNAL   J Acquir Immune Defic Syndr Hum Retrovirol 12 (2), 128-138 (1996)
   PUBMED   8680883
REFERENCE   7  (bases 1 to 8361)
  AUTHORS   Parada,C.A., Yoon,J.B. and Roeder,R.G.
  TITLE     A novel LBP-1-mediated restriction of HIV-1 transcription at the
            level of elongation in vitro
  JOURNAL   J Biol Chem 270 (5), 2274-2283 (1995)
   PUBMED   7836461
REFERENCE   8  (bases 1 to 8361)
  AUTHORS   Zhou,Q. and Sharp,P.A.
  TITLE     Novel mechanism and factor for regulation by HIV-1 Tat
  JOURNAL   EMBO J 14 (2), 321-328 (1995)
   PUBMED   7835343
REFERENCE   9  (bases 1 to 8361)
  AUTHORS   Ou,S.H., Garcia-Martinez,L.F., Paulssen,E.J. and Gaynor,R.B.
  TITLE     Role of flanking E box motifs in human immunodeficiency virus type
            1 TATA element function
  JOURNAL   J Virol 68 (11), 7188-7199 (1994)
   PUBMED   7933101
REFERENCE   10 (bases 1 to 8361)
  AUTHORS   Kashanchi,F., Piras,G., Radonovich,M.F., Duvall,J.F., Fattaey,A.,
            Chiang,C.M., Roeder,R.G. and Brady,J.N.
  TITLE     Direct interaction of human TFIID with the HIV-1 transactivator tat
  JOURNAL   Nature 367 (6460), 295-299 (1994)
   PUBMED   8121496
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB164703.1, AF038362.2 and AL365398.22.
            
            On Nov 20, 2018 this sequence version replaced NM_003972.2.
            
            Summary: This gene encodes a TAF (TATA box-binding
            protein-associated factor), which associates with TBP (TATA
            box-binding protein) to form the B-TFIID complex that is required
            for transcription initiation of genes by RNA polymerase II. This
            TAF has DNA-dependent ATPase activity, which drives the
            dissociation of TBP from DNA, freeing the TBP to associate with
            other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep
            2011].
            
            Transcript Variant: This variant (1) represents the protein-coding
            transcript.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.90570.1,
                                           SRR1803617.187653.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265990.12/ ENSP00000265990.6
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               DB164703.1         2-210
            210-6457            AF038362.2         1-6248
            6458-8361           AL365398.22        40744-42647
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q23.32"
     gene            1..8361
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="B-TFIID TATA-box binding protein associated factor
                     1"
                     /db_xref="GeneID:9044"
                     /db_xref="HGNC:HGNC:17307"
                     /db_xref="MIM:605191"
     exon            1..321
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..5857
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="TBP-associated factor 172; B-TFIID transcription
                     factor-associated 170 kDa subunit; ATP-dependent helicase
                     BTAF1; BTAF1 RNA polymerase II, B-TFIID transcription
                     factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)"
                     /codon_start=1
                     /product="TATA-binding protein-associated factor 172"
                     /protein_id="NP_003963.1"
                     /db_xref="CCDS:CCDS7419.1"
                     /db_xref="GeneID:9044"
                     /db_xref="HGNC:HGNC:17307"
                     /db_xref="MIM:605191"
                     /translation="MAVSRLDRLFILLDTGTTPVTRKAAAQQLGEVVKLHPHELNNLL
                     SKVLIYLRSANWDTRIAAGQAVEAIVKNVPEWNPVPRTRQEPTSESSMEDSPTTERLN
                     FDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEA
                     IGMSTEELFNDEDLDYTPTSASFVNKQPTLQAAELIDSEFRAGMSNRQKNKAKRMAKL
                     FAKQRSRDAVETNEKSNDSTDGEPEEKRRKIANVVINQSANDSKVLIDNIPDSSSLIE
                     ETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQ
                     HQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVD
                     VLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVA
                     AASLVPVVESLVYLQTQKVPFIINTLWDALLELDDLTASTNSIMTLLSSLLTYPQVQQ
                     CSIQQSLTVLVPRVWPFLHHTISSVRRAALETLFTLLSTQDQNSSSWLIPILPDMLRH
                     IFQFCVLESSQEILDLIHKVWMELLSKASVQYVVAAACPWMGAWLCLMMQPSHLPIDL
                     NMLLEVKARAKEKTGGKVRQGQSQNKEVLQEYIAGADTIMEDPATRDFVVMRARMMAA
                     KLLGALCCCICDPGVNVVTQEIKPAESLGQLLLFHLNSKSALQRISVALVICEWAALQ
                     KECKAVTLAVQPRLLDILSEHLYYDEIAVPFTRMQNECKQLISSLADVHIEVGNRVNN
                     NVLTIDQASDLVTTVFNEATSSFDLNPQVLQQLDSKRQQVQMTVTETNQEWQVLQLRV
                     HTFAACAVVSLQQLPEKLNPIIKPLMETIKKEENTLVQNYAAQCIAKLLQQCTTRTPC
                     PNSKIIKNLCSSLCVDPYLTPCVTCPVPTQSGQENSKGSTSEKDGMHHTVTKHRGIIT
                     LYRHQKAAFAITSRRGPTPKAVKAQIADLPAGSSGNILVELDEAQKPYLVQRRGAEFA
                     LTTIVKHFGGEMAVKLPHLWDAMVGPLRNTIDINNFDGKSLLDKGDSPAQELVNSLQV
                     FETAAASMDSELHPLLVQHLPHLYMCLQYPSTAVRHMAARCVGVMSKIATMETMNIFL
                     EKVLPWLGAIDDSVKQEGAIEALACVMEQLDVGIVPYIVLLVVPVLGRMSDQTDSVRF
                     MATQCFATLIRLMPLEAGIPDPPNMSAELIQLKAKERHFLEQLLDGKKLENYKIPVPI
                     NAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHCHRAQEYARSK
                     LAECMPLPSLVVCPPTLTGHWVDEVGKFCSREYLNPLHYTGPPTERIRLQHQVKRHNL
                     IVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGKTKLSKAVKQLTANYRIILSGTPI
                     QNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDARSSSREQEAGVLAMDALHR
                     QVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQLYEDFAKSRAKCDVDETVSSAT
                     LSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEFKTTAEKLAVQNSSLHDI
                     QHAPKLSALKQLLLDCGLGNGSTSESGTESVVAQHRILIFCQLKSMLDIVEHDLLKPH
                     LPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEH
                     DWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMNIANTVISQENS
                     SLQSMGTDQLLDLFTLDKDGKAEKADTSTSGKASMKSILENLSDLWDQEQYDSEYSLE
                     NFMHSLK"
     exon            322..445
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            446..560
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            561..707
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            708..871
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            872..1008
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1138
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1207
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1297
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1393
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1570
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1711
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1836
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1957
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2062
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..2212
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2355
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            2356..2530
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            2531..2734
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..3161
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            3162..3352
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3506
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            3507..3616
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            3617..3818
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            3819..3967
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            3968..4120
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4121..4242
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4243..4410
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4411..4488
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4489..4618
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4619..4760
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4761..4891
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            4892..5017
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            5018..5170
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            5171..5382
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            5383..5542
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            5543..5713
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     exon            5714..8361
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7227..7232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="hexamer: AATATA"
     polyA_site      7249
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="major polyA site"
     regulatory      7899..7904
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="hexamer: ATTAAA"
     polyA_site      7922
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="major polyA site"
     regulatory      8087..8092
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="hexamer: AATATA"
     polyA_site      8105
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
     regulatory      8340..8345
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /note="hexamer: AGTAAA"
     polyA_site      8361
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
ORIGIN      
        1 agttgtgcgt gccgacgccc gcgtcagcaa agagcggagc tgagggtacc cggtttgaag
       61 tcgtgcgggt cggaggactg ccgcctccgc taccgtcttg gacccctgct taccggccgc
      121 cgcggggacg agctcgggta ggcggcaggg cagatgcccg agggcctgcg ctggaacgcc
      181 ccacgccgca ccggccgctc ccctgtgctc cgcggcctgg gcctgcgccg ctcagctctc
      241 tggaaactag cgcctcagct gcgcggcgcg taggtcgcgg ggagctccga accgccggcg
      301 cccggccatg gcggtctcca ggctagatcg cctttttatt ttactggata ctggcactac
      361 tcctgttaca agaaaagctg ctgcacagca acttggagaa gtggtgaagc ttcatcccca
      421 tgaactaaat aatctcctgt ctaaagtgtt gatatattta aggagtgcaa attgggatac
      481 ccggattgca gcaggacaag ctgttgaagc tatagtgaaa aatgtacctg agtggaatcc
      541 agtgccgaga accagacaag aacctacttc cgaaagttct atggaagatt cacctactac
      601 agagcgattg aattttgaca gatttgatat atgtagattg ttacaacatg gtgcatcact
      661 cctgggatct gctggtgccg aatttgaagt ccaagatgaa aaatcaggtg aagtggatcc
      721 taaagagagg atagcacgcc aacgaaaatt attacagaag aaacttggcc ttaatatggg
      781 agaagcaatt ggaatgagta ctgaagaact tttcaatgat gaggatttgg attatacccc
      841 aacttcagca tcctttgtta acaaacaacc tactcttcag gcagctgaat tgattgactc
      901 agagtttcga gcaggaatga gcaatagaca aaagaacaaa gctaaaagaa tggccaagtt
      961 atttgcaaaa cagagatcca gggatgcagt ggaaactaat gagaagagca atgatagcac
     1021 tgatggggag ccagaagaaa agagacggaa aatagcaaat gttgttatta atcagtctgc
     1081 aaatgattcc aaagtcttga ttgataatat tccagacagc tcttccttaa ttgaagagac
     1141 aaatgaatgg cctttggaaa gcttttgtga agaactttgc aatgaccttt ttaatccctc
     1201 ctgggaggtt cgacatggtg caggcactgg acttagggaa attcttaaag ctcatgggaa
     1261 aagtggtggt aaaatgggtg acagcacttt agaagagatg attcagcagc atcaagagtg
     1321 gttggaagac ttggttatta gactcctttg tgtttttgca ttagacagat ttggagactt
     1381 tgtttctgat gaagttgtgg caccagttcg tgaaacttgt gctcaaacat taggtgtggt
     1441 tttaaaacac atgaacgaaa caggagttca taagactgtg gatgtgctgc taaaattact
     1501 tacacaagaa caatgggaag ttagacatgg tggtctgctg ggaataaaat atgctttggc
     1561 agtccgtcag gatgtaatta atactttatt gcctaaagtt ttaactagaa taattgaagg
     1621 actccaggat cttgatgatg atgtcagagc tgttgctgca gcatcattag tgcctgtagt
     1681 agaaagcctt gtctatcttc agacacaaaa ggtgcccttc attataaata cattgtggga
     1741 tgctcttctg gaattagatg atctaacagc ttcaacaaat agtattatga ctctcctttc
     1801 atccttgtta acttaccctc aggtccaaca atgcagtatt cagcagtcac tgacagtttt
     1861 agttccacgt gtctggcctt ttttgcatca cactatatca tcagttcgaa gagcagcatt
     1921 ggaaactctg tttacgttat tatcaacaca ggaccagaac tcttcatctt ggcttatacc
     1981 tatactgcct gatatgctcc gacacatttt tcagttctgt gttttggaaa gcagccagga
     2041 aattctggac cttattcaca aggtttggat ggaactgttg agtaaggctt cagttcagta
     2101 tgtggtagca gctgcttgcc catggatggg tgcttggctt tgcttgatga tgcagccttc
     2161 tcatttacct atcgatttaa atatgttgct agaagtaaaa gctagagcca aggaaaaaac
     2221 aggtggtaag gtgcgccaag gccaaagcca gaataaagaa gtacttcagg agtatattgc
     2281 aggtgccgac accatcatgg aagacccagc caccagggat tttgtagtta tgcgggccag
     2341 aatgatggca gccaagttgt taggagcact ttgttgctgt atttgtgatc caggtgtaaa
     2401 tgtggtaact caagaaatta aaccagctga atccctgggc cagttactac tcttccattt
     2461 gaactccaag tctgctttac agaggattag tgtagctttg gtaatctgtg aatgggctgc
     2521 tttacaaaag gagtgtaaag ctgttacctt agctgtgcag ccgcgtttac ttgatatcct
     2581 ttcagaacat ttatattatg acgaaattgc cgttccattc acacgaatgc agaatgaatg
     2641 taaacaactt atatcatcat tagctgatgt acatattgaa gttggtaatc gagtaaacaa
     2701 caatgtttta acaatagatc aagctagtga cttggtcact actgttttta atgaagccac
     2761 atcatctttc gatttaaatc ctcaagtgtt acaacagtta gatagtaaac gacagcaggt
     2821 ccaaatgaca gttacagaga ccaaccagga gtggcaagtg ttgcagttga gagtgcatac
     2881 ttttgctgcc tgtgcagttg tgagcttgca gcagcttccg gagaaattaa atcctatcat
     2941 aaaaccatta atggagacaa ttaaaaaaga agagaataca ctagtgcaaa actatgcagc
     3001 tcagtgcata gctaaactcc ttcagcagtg cacaacaagg acgccctgtc ccaattcaaa
     3061 aattattaaa aacctctgta gctcactttg tgtggaccca tatctaactc cttgtgtcac
     3121 atgtccagta ccaacacaaa gtggccagga aaattctaaa ggatccacct cagaaaaaga
     3181 tggaatgcac catactgtca ccaagcacag aggtataatt acactctaca ggcaccagaa
     3241 agctgccttt gctatcacaa gtaggcgagg tcctaccccc aaagcagtaa aagctcaaat
     3301 agcagatctt cctgcaggaa gtagtggaaa tattcttgtt gaacttgatg aggcccagaa
     3361 gccttacctg gtacaacgga gaggagctga atttgctttg acaactatag taaagcattt
     3421 tggtggtgaa atggcagtga agttgccaca tctctgggat gctatggttg gcccattgag
     3481 gaatacaatc gacataaata attttgatgg aaaatccctc ctggataagg gagatagccc
     3541 tgctcaagaa ttggtgaatt ctctgcaggt ttttgaaaca gcagcagctt caatggattc
     3601 tgagcttcat cccttgttgg tccagcattt gccacatctt tatatgtgcc ttcaataccc
     3661 cagtactgca gtgaggcaca tggctgctcg ttgtgtaggt gtcatgagca aaatagctac
     3721 catggaaaca atgaatattt ttttggagaa ggttcttccg tggctgggag caattgatga
     3781 cagtgtcaaa caagagggtg caattgaagc acttgcctgt gtaatggaac aactagatgt
     3841 tggtattgtt ccatatattg tccttttagt tgttcctgta ttaggaagaa tgagtgatca
     3901 gacagacagt gtgagattca tggccacgca gtgctttgca acgctaatta gactcatgcc
     3961 acttgaggca ggcattccag accctccaaa catgtcagca gaattaatcc aattgaaagc
     4021 caaggagcga cactttttgg agcaattgtt agatgggaaa aaattggaaa attataaaat
     4081 tccagtacca atcaatgctg aactcagaaa atatcagcag gatggtgtga actggttagc
     4141 atttcttaat aagtataaac ttcatggaat tctgtgtgat gacatgggtt taggaaaaac
     4201 tttacagtcc atctgcattc tagcaggaga tcattgtcat agggcccagg aatatgcaag
     4261 atcaaaatta gcagaatgta tgccacttcc ttccttagtg gtttgtccgc caacattaac
     4321 aggccattgg gtggatgaag taggtaaatt ttgctctaga gaatatctca acccgttgca
     4381 ttacactgga cctcccactg aaagaataag gttacagcac caagtaaaaa ggcacaatct
     4441 aatagtggct tcatatgatg ttgtgaggaa tgacatagat ttctttagaa atattaaatt
     4501 taactactgc attcttgatg aaggccatgt catcaaaaat ggaaaaacaa agttgtcaaa
     4561 agcagtaaaa caactgactg ctaattatag gattattctt tctggaacac caatccagaa
     4621 caacgttttg gagctgtggt cattatttga tttcctcatg ccaggatttt tgggtactga
     4681 acgccagttt gctgctcgat atggtaaacc tatattagca agtagggatg ctcgaagctc
     4741 cagtcgagag caagaagcag gtgttcttgc tatggatgcg ctgcaccgcc aagtactacc
     4801 gtttcttttg agaagaatga aagaagatgt tttgcaggat cttccaccta aaattattca
     4861 agactattat tgtactctta gtcctctcca ggttcagctc tatgaagatt ttgctaagtc
     4921 tcgtgccaag tgtgatgttg atgaaacagt ttcttcagct acactttctg aagaaactga
     4981 aaaaccaaag cttaaagcta caggccacgt attccaggca ttacagtact tacgtaaact
     5041 gtgcaaccat ccagcattag tcttaacacc tcaacatcca gaattcaaga ccactgccga
     5101 aaaactggca gttcagaatt cttctctaca tgatattcaa catgccccta agctctcagc
     5161 tttgaaacaa ttgctgttgg actgcggttt gggaaatggc agcacttccg agagtggcac
     5221 agagtctgtt gtggcccagc acaggatact gatattctgt cagctgaaaa gcatgcttga
     5281 tatagtagag catgatctcc tcaaacctca cttgccctct gtcacttatt tgagattaga
     5341 tggcagcata cctcctggtc agaggcattc cattgtttcc cggtttaata atgatccatc
     5401 tatagacgtt ctgttactta ccactcacgt tggtggcctg ggacttaatt tgacaggcgc
     5461 tgacacagta gtatttgtgg agcatgactg gaatcctatg cgagatctac aagccatgga
     5521 ccgggcccat cgcattgggc agaaacgtgt ggttaacgta taccgattga taaccagagg
     5581 aacattggaa gaaaaaataa tggggttgca gaaattcaag atgaacatag cgaatactgt
     5641 tattagccaa gagaattcta gtttgcagag catggggact gatcagcttc ttgatctgtt
     5701 tactcttgat aaggatggca aagcagaaaa agctgacacc tctacttctg ggaaagcaag
     5761 tatgaaatca attcttgaaa acctgagtga tctttgggat caagagcagt atgattcaga
     5821 gtacagcctg gaaaatttta tgcattctct caagtaacta tcaaatattg taaatgcaat
     5881 tgctgctagt tcagttacat ttctgctgat attcagcaaa tttctaagtt tatggtgaac
     5941 ttttaactca atgtgtaaat agatctggag aatattcagc ataatgctgg ctcttgtttc
     6001 actgggggaa acaagttatt ctcaaatatt tgcactgtat taaatttaaa tgttaatgtg
     6061 aaatggttta aattttacat gctgaaaagc tgcagagcag aggaaccaaa ccaggtttat
     6121 ttgtgctacc aggaggtgtt cttaatggga acaggcctcc tatctttagt cactttgtac
     6181 aggatgatat ccatgagtac tttagtgttt gtatatgttt tttcttttaa atagaacttg
     6241 tttttataat tgatttaaaa tagggctttt tttctataaa agccttaatg agccataatt
     6301 ttaagaatat aagaataatg actatgatat tggtcagtct tagaacatga aaatgtcaga
     6361 caatgaattt aatcgggccc ttggtggaaa catttatata tttaatgcag atgcatagtg
     6421 tttttcaaat ctttaacatc attttttcaa cttttaagat ataatatcaa ctattctaaa
     6481 tacaggtaat ggtatattta aggctaccat aacatcctat ttagagggtg gtttttttta
     6541 atttatctaa tggctacgat atagccagat tcaaataaca tatgtactca ataggtttca
     6601 atcatatata taatatattt tttgtaacta tgaacataca cagttttcca gaaatagatt
     6661 ttacactgtt tagaattcca acacctacag tggaaagact gtttattgta gtaatgcatg
     6721 gctgaagcat aaaagggaga gagaatttct ttatatacac aaacatacat gaatatgcat
     6781 atctatatgc atagatgtac ctatcctgca cccaaaaagg tgcctggtat tggcactaaa
     6841 atcatgtagt tatactgggc agcaataata attgggcatg aggctgatta ctcaatggtg
     6901 agggtaggta tatgtagata gatgtgcatg tatgtgtttg tatatataat tttataagtt
     6961 tcacgcataa attaatacat gcctgtgtgc atacatatgt atgggatata tttgtatata
     7021 tatgtacagg taataaacat ccccaaggtt tgtggctggc catacacata ggcatcagtt
     7081 taacaaccat cagacctcag ctgtacaata acaggtgttt tgtttaatta aaattatact
     7141 gttctgcagc atttagacat ttgtcacatt tcattagctt tgacaaccat actgtaacat
     7201 taaacctagc attccacaag agaataaata taagattgaa actgtaaaat atatgcaact
     7261 gtgtgttatt tgtaggaaat ggtcactaga aagagcaaaa gattattcag gtatacaggt
     7321 tttttttcat tgtttaggat tttaactcaa aactttatgt agaacatttc agtatatttg
     7381 agttgaatat ttttttatta actatttatt tgctagttcc ataaaccagc tctttaacat
     7441 agcttatagt aattgatatt ttcttagaat aagtacagag caatatatca ttaaagggct
     7501 atgaaggggc aaaagaaatc acaccataaa agttagtact ttttcccaat ctgaaatgtc
     7561 agatagagct agaagtcaga tatttaggct gtttaaactt tctgagagtt taatcaaaag
     7621 gtgtgtattt ctagaaggat gactagaaaa atgatgtgtg gttgcttctg aatgggaaac
     7681 aggagtcaga gctgacagga aaacttagag tggctataac atttttaaaa taatcttttg
     7741 agttgtaaaa tagtaaatca cacattccaa cttttccata gaaaggaaaa catgtaaaca
     7801 ggtaagaact aagtattctt aattaaatga cccagtcatt gtagtacatc ttaagaataa
     7861 tgaagagatg tggagaattg aactgagtaa tgaatgatat taaagaactg ctgtcaattt
     7921 catggatgtg atgatttggt agttttttca atgtatgggt gtgggaagtc aaacaagcat
     7981 gatagaatgt taactatttt aaactgggaa ttgggttcat agatatccat tgtactactt
     8041 cccttggttt ttgtgtcttt atgataggaa atagcaaact gttaaaaata tagattcaga
     8101 gattattagt cttatcattt gggtttcttg taggaaaatc ttaacatttg ggggcctggg
     8161 tataattttt gtttcatata ttcactcata tatatgctta ttgtatatgc ttatttattc
     8221 tacaaacatt tctccaaaaa gtattctagt gaaagggtaa aagtctttga tgttgggact
     8281 gattgaactg gtatagcctt attccttctg gatggtttat atgaacttac ttattcaaaa
     8341 gtaaatattc cactgaagct a
//
