LOCUS       NM_057176               9761 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens barttin CLCNK type accessory subunit beta (BSND),
            mRNA.
ACCESSION   NM_057176
VERSION     NM_057176.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Gorinski,N., Wojciechowski,D., Guseva,D., Abdel Galil,D.,
            Mueller,F.E., Wirth,A., Thiemann,S., Zeug,A., Schmidt,S.,
            Zareba-Koziol,M., Wlodarczyk,J., Skryabin,B.V., Glage,S.,
            Fischer,M., Al-Samir,S., Kerkenberg,N., Hohoff,C., Zhang,W.,
            Endeward,V. and Ponimaskin,E.
  TITLE     DHHC7-mediated palmitoylation of the accessory protein barttin
            critically regulates the functions of ClC-K chloride channels
  JOURNAL   J Biol Chem 295 (18), 5970-5983 (2020)
   PUBMED   32184353
  REMARK    GeneRIF: DHHC7-mediated palmitoylation of the accessory protein
            barttin critically regulates the functions of ClC-K chloride
            channels.
REFERENCE   3  (bases 1 to 9761)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 9761)
  AUTHORS   Wojciechowski,D., Thiemann,S., Schaal,C., Rahtz,A., de la Roche,J.,
            Begemann,B., Becher,T. and Fischer,M.
  TITLE     Activation of renal ClC-K chloride channels depends on an intact N
            terminus of their accessory subunit barttin
  JOURNAL   J Biol Chem 293 (22), 8626-8637 (2018)
   PUBMED   29674316
  REMARK    GeneRIF: Increasing expression of barttin over that of ClC-K
            partially recovered this insufficiency, indicating that N-terminal
            modifications of barttin alter both binding affinities and gating
            properties
REFERENCE   5  (bases 1 to 9761)
  AUTHORS   Shinmura,K., Kato,H., Kawanishi,Y., Kamo,T., Inoue,Y.,
            Yoshimura,K., Sugiyama,K., Misawa,K., Hosokawa,S., Mineta,H. and
            Sugimura,H.
  TITLE     BSND is a Novel Immunohistochemical Marker for Oncocytic Salivary
            Gland Tumors
  JOURNAL   Pathol Oncol Res 24 (2), 439-444 (2018)
   PUBMED   28470573
  REMARK    GeneRIF: These results suggest that BSND is expressed only in
            normal salivary glands and oncocytic salivary gland tumors such as
            Warthin's tumor and oncocytoma
REFERENCE   6  (bases 1 to 9761)
  AUTHORS   Miyamura,N., Matsumoto,K., Taguchi,T., Tokunaga,H., Nishikawa,T.,
            Nishida,K., Toyonaga,T., Sakakida,M. and Araki,E.
  TITLE     Atypical Bartter syndrome with sensorineural deafness with G47R
            mutation of the beta-subunit for ClC-Ka and ClC-Kb chloride
            channels, barttin
  JOURNAL   J Clin Endocrinol Metab 88 (2), 781-786 (2003)
   PUBMED   12574213
REFERENCE   7  (bases 1 to 9761)
  AUTHORS   Waldegger,S., Jeck,N., Barth,P., Peters,M., Vitzthum,H., Wolf,K.,
            Kurtz,A., Konrad,M. and Seyberth,H.W.
  TITLE     Barttin increases surface expression and changes current properties
            of ClC-K channels
  JOURNAL   Pflugers Arch 444 (3), 411-418 (2002)
   PUBMED   12111250
REFERENCE   8  (bases 1 to 9761)
  AUTHORS   Estevez,R., Boettger,T., Stein,V., Birkenhager,R., Otto,E.,
            Hildebrandt,F. and Jentsch,T.J.
  TITLE     Barttin is a Cl- channel beta-subunit crucial for renal Cl-
            reabsorption and inner ear K+ secretion
  JOURNAL   Nature 414 (6863), 558-561 (2001)
   PUBMED   11734858
REFERENCE   9  (bases 1 to 9761)
  AUTHORS   Birkenhager,R., Otto,E., Schurmann,M.J., Vollmer,M., Ruf,E.M.,
            Maier-Lutz,I., Beekmann,F., Fekete,A., Omran,H., Feldmann,D.,
            Milford,D.V., Jeck,N., Konrad,M., Landau,D., Knoers,N.V.,
            Antignac,C., Sudbrak,R., Kispert,A. and Hildebrandt,F.
  TITLE     Mutation of BSND causes Bartter syndrome with sensorineural
            deafness and kidney failure
  JOURNAL   Nat Genet 29 (3), 310-314 (2001)
   PUBMED   11687798
REFERENCE   10 (bases 1 to 9761)
  AUTHORS   Shearer,A.E., Hildebrand,M.S., Odell,A.M. and Smith,R.J.H.
  TITLE     Genetic Hearing Loss Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK129999.1, AY034632.1,
            AL590440.1 and AL589790.22.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 20, 2018 this sequence version replaced NM_057176.2.
            
            Summary: This gene encodes an essential beta subunit for CLC
            chloride channels. These heteromeric channels localize to
            basolateral membranes of renal tubules and of potassium-secreting
            epithelia of the inner ear. Mutations in this gene have been
            associated with Bartter syndrome with sensorineural deafness.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK129999.1, SRR1803617.60777.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968832, SAMEA1970526
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000651561.1/ ENSP00000498282.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AK129999.1         1-11
            12-137              AY034632.1         1-126
            138-185             AL590440.1         59958-60005
            186-1238            AY034632.1         175-1227
            1239-9761           AL589790.22        8525-17047
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p32.3"
     gene            1..9761
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /note="barttin CLCNK type accessory subunit beta"
                     /db_xref="GeneID:7809"
                     /db_xref="HGNC:HGNC:16512"
                     /db_xref="MIM:606412"
     exon            1..431
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /inference="alignment:Splign:2.1.0"
     CDS             255..1217
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /note="deafness, autosomal recessive 73; Bartter syndrome,
                     infantile, with sensorineural deafness (Barttin); barttin
                     CLCNK-type chloride channel accessory beta subunit;
                     barttin CLCNK type accessory beta subunit"
                     /codon_start=1
                     /product="barttin"
                     /protein_id="NP_476517.1"
                     /db_xref="CCDS:CCDS602.1"
                     /db_xref="GeneID:7809"
                     /db_xref="HGNC:HGNC:16512"
                     /db_xref="MIM:606412"
                     /translation="MADEKTFRIGFIVLGLFLLALGTFLMSHDRPQVYGTFYAMGSVM
                     VIGGIIWSMCQCYPKITFVPADSDFQGILSPKAMGLLENGLAAEMKSPSPQPPYVRLW
                     EEAAYDQSLPDFSHIQMKVMSYSEDHRSLLAPEMGQPKLGTSDGGEGGPGDVQAWMEA
                     AVVIHKGSDESEGERRLTQSWPGPLACPQGPAPLASFQDDLDMDSSEGSSPNASPHDR
                     EEACSPQQEPQGCRCPLDRFQDFALIDAPTLEDEPQEGQQWEIALPNNWQRYPRTKVE
                     EKEASDTGGEEPEKEEEDLYYGLPDGAGDLLPDKELGFEPDTQG"
     exon            432..526
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /inference="alignment:Splign:2.1.0"
     exon            527..802
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /inference="alignment:Splign:2.1.0"
     exon            803..9761
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1356..1361
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /note="hexamer: AATACA"
     polyA_site      1378
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /note="major polyA site"
     regulatory      3448..3453
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /note="hexamer: AATAAA"
     polyA_site      3472
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
     regulatory      9742..9747
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
                     /note="hexamer: AATAAA"
     polyA_site      9761
                     /gene="BSND"
                     /gene_synonym="BART; DFNB73"
ORIGIN      
        1 agagggaagg tgagagggca aggagtaaag gtggctgggt gtgggtccgt tgaagcgagc
       61 cgcctccagc cctgttgaac tggtgggccc agggactgga gcgggattga aagggatctt
      121 gctctccctt gaagccttga gttgcagcga tttcagtgtc ttctctccct gtgtaagcct
      181 gtctcggtgt ttaggctgaa ctacagccac cccctctccc gggggtgtgc aggccaggga
      241 ctggccaggc agccatggct gacgagaaga ccttccggat cggcttcatt gtgctggggc
      301 ttttcctgct ggccctcggt acgttcctca tgagccatga tcggccccag gtctacggca
      361 ccttctatgc catgggcagc gtcatggtga tcgggggcat catctggagc atgtgccagt
      421 gctaccccaa gatcaccttc gtccctgctg actctgactt tcaaggcatc ctctccccaa
      481 aggccatggg cctgctggag aatgggcttg ctgccgagat gaagagcccc agtccccagc
      541 cgccctatgt aaggctgtgg gaggaagccg cctatgacca gagcctgcct gacttcagcc
      601 acatccagat gaaagtcatg agctacagtg aggaccaccg ctccttgctg gcccctgaga
      661 tggggcagcc gaagctggga accagtgatg gaggagaagg tggccctggc gacgttcagg
      721 cctggatgga ggctgccgtg gtcatccaca agggctcaga cgagagtgaa ggggaaagac
      781 gcctaactca gagctggccc ggccccctgg cctgtcccca gggccctgcc cccttggctt
      841 ccttccaaga tgacctggac atggactcca gtgaaggcag cagccccaat gcatctccac
      901 atgacaggga ggaagcttgt tccccacaac aggaacctca gggctgcagg tgcccgctgg
      961 accgcttcca agactttgcc ctgattgatg ccccaacgtt ggaggatgag ccccaagagg
     1021 ggcagcagtg ggaaatagcc ctgcccaaca actggcagcg gtacccaagg acaaaggtgg
     1081 aggagaagga ggcttcggac acaggtgggg aggaacctga gaaggaagag gaagacctgt
     1141 actatgggct gccagatgga gccggggacc tcctcccgga caaggagctg ggttttgagc
     1201 ctgacaccca aggctgagat gtttgtgctc cgtagcttct agtctggaac tgctgctgac
     1261 ccctgtgtga catcacaggg cctcagtttc cctatctgca aaatgggatg atatggaggt
     1321 tcaatgagat ggtggcattt tgagaatggt aaagaaatac acagggaggg gatgatgact
     1381 attagtggac tcttgttttt ccaatagtta gtggtctttg gccaaccttt gaaggcaaaa
     1441 tatgatttgt tgaggtttgc agcagctgaa gccctcaaaa gcattttcca ccttccccca
     1501 aagctgacct ctgagaggaa ccttccttgg tgctccctct gggcccctga tcctttgtga
     1561 aggccagtca ggtttcccat tcctattgtg tcttatagtc cacttggtag atggcgtgat
     1621 ccgtctttac acagagggag cctcatctcc tcctgggcca cattcctcct gcagctccca
     1681 tcgctccttg gccaggaccc aaaaccttct gtgactgacc cctgctgacc tcattctctc
     1741 tgctcctcca gttccaagcc tagcccccct gcccatcttg cggctcacac tcttgcccaa
     1801 gctgttctcc tccaccagga tgcccttggc tcccttttca gcttggcaaa ttcgtgctca
     1861 cccttcaaac ccctacacag atgacctccc ttggaaaggt tttcctgatg tcgctgcctc
     1921 ctggaaccag gtggtcttgc ctctctgcgc tgcctttggg acctgtatat ttgtcagtgt
     1981 ggcatggctg cctgcctccc gttgaagggc agcatcttga ctttactcac cttgagatcg
     2041 cctgtgccca gatgggcctt tggcctggcc cagaatcggt ggtcatcaag ggtgtacaga
     2101 actcaccagg acccaggaac ctgttgcaga taaaatcact ctcccccacg ttcccaactt
     2161 ttagactcac agccctctta cttcccagca ttcaaacttc taaatggcct gtatgtattc
     2221 acaaagcagg caaaaagccc ccagaggaac accacccccc acccctgcct ctggaataca
     2281 tttgctttga cttggaattg taggtgaaat tcatcttcct ggcctacttc agtttgaatc
     2341 agttggctag ccaggtgcgg tggctcatgc ctgtaatctg agcattttgg gaggctaaag
     2401 tgggtggatc acttgaggcc aggagttgga gagcagcctg ggcaacatag cgaaacccta
     2461 tctctactaa aactacaaaa attagctggg cgtggtggtg cacacctgta gtctcagcta
     2521 cttgggaggc tgagctggag gatagcttaa gcacaggaga tggaagttgc agtgagccga
     2581 gatcgcacca ctgcactccg gcctgggtga cagagtgaga ccctgcctca aaacaaaaca
     2641 attggaattg gatggcattt agtgagcatc tactatatgc cagatattga gctgtgatct
     2701 ctaggggtaa agagacatga actactgaca ccgtgcttgc cttcagaggg ctcacaatct
     2761 agtgagcttg atttctgtga gtgcagagca gaagctgagg tgggaacaga gaagtaaaaa
     2821 atggacacac cagaggcaga agagtgacag cctcccagct gtttggggca gttgagtatg
     2881 gtggggaaat agctcaagct gtctttgcag cccctgaaga catcactgta ccctattgaa
     2941 ccatctcagg gtgatgctat tgttacgctg atccctgcaa cgcttgcctg tgccccagcc
     3001 caggatcctg gcagctgcag agaaaatggc agtaacacta cctcaaacca ggaccacgcc
     3061 ctcagagcct gcaccctggg ccacctggga gtcagagacc ttcacctggg ctatggggtt
     3121 ctgggttgag gctggggttc ccggcctagg gaagggcttc cgggttgagg ccgggggtcc
     3181 tggcctaggg aaggagatgc ccctgtgtgc tgggttacaa ggtcatggcc ctgggaacac
     3241 acttcctttt ctttctgggt catttctgcc tctgcagtga aggatgccac agggtacacc
     3301 ctctctgctg tcatgactca ggacaggagt tcctggtcac agctgagtct tcggatgagg
     3361 tgaggggtag catgctcctg tgctctgttg cctaaggcag ggcagcctac aacccacgtt
     3421 agaaacagcg tcagcactaa gggctaaaat aaaactactt tcaacattta aaggggtcat
     3481 cttcattcac ctgcaaattc ttttctttaa aaccacccct tccctcgggg caggatacgt
     3541 gaggccttac gcctgacggg caccagaggc cctcttccgg aagtaagccc tcactgcagt
     3601 ggcatgaccg tggggtgtgc tgtgtggtgc tgaatctcag gagtgattcc ccacacccct
     3661 gaaccacaac cgcagtggct ttagacttaa acaagagcct ctggttgctg ggcctgccag
     3721 caggaagatg gtaaccctgt tctagagtta gcatctgcag ggggcttggg gtagtggagt
     3781 gagatggtga gacccaggct tggtctccct ggctcttgct gcatgatggg gagaccacac
     3841 acctgatctg cccaggaagg tcccggttga caccgactgc ctctgctaag ttattaggag
     3901 gcccctttca cgctcgaaga tacctcattt ggatgataca atgttacctt acctactctg
     3961 tgacctttga caagtctggt tgcctccgta gccctcagct ttctcatgtg caaatgacag
     4021 atgaagtgcg catatcttgg aatcttggaa gtgatggggg tgtttgatgg gacctatctt
     4081 gtcaatgccc tgcagccaaa gaccactggg agcacacctc acctgtggtt aacaagctgg
     4141 gtttcttctt gcagtaaggg ggaatgaaca ccacagcgaa ccagggggag tctctgaaag
     4201 aggtattaga aaggacttgt tactggattt gggccagtgc tcagtggctt ggcgagggtt
     4261 taagaagcag gcctttgctc tgaaccggct gctgtcagga ggcggggctc attctatgac
     4321 tgggtgtctt cttcaacctt atctaggata aaataaaatc ataaaacact gtgcttcatg
     4381 cccctggcct tgccaacttg cagtcccctc taagagcatc agagcctctg gggcaccccc
     4441 tctccactgg gagaggggca aggtgctggt gaaggtcctg gggagaaggc tgggaggctc
     4501 tgaaagggtc aggaggggcc ttttggggtg gcaaagcctg ggattctagc gttcagctcc
     4561 aggctccagg tgtgtgtgcc agggtgaaaa ttcctgctct tggtcactct cctgtcaggt
     4621 tggagggact ctggaagttt ccaccctctg gagagggaaa ggccctggag ggatggcccc
     4681 tgcccttaac tggaaagggt gggcaaggga aggcaggggg cccaggggca gggaacctgg
     4741 gctctgccga ccgctcgcca tgaccttggg tacatcacag ccctccctgg gctcctctat
     4801 tctctgtaaa attgggtgag ggtgggcaag attccacttc tgctcccagt ccctgagcac
     4861 tgtgtgcctg tcaccatgca agacaccgtc acccctatta ccctatctag tccgtgaaac
     4921 agccctgggg gaccgatctg tcgaccccac attgcaaatg tttgttataa atcccaggct
     4981 ccagaatcag aaagacctgg ttttaccttc agcctcagcc agttccttgc tgtgtggcct
     5041 tggacaagtc acagcccctc tcagagcctc aatgtccaca tttatgcagt ggggaggata
     5101 atacctccct cttgggcttt tgtggagaag aactgggtat aatgcctcag gcacatttag
     5161 gtgcttagac agtggtggtc ttcttccctc tctgggtctc ttccctcatc tatgcatcag
     5221 ggagccactg tcctgcctat aaataaaaca ataggtgagt gcattcacca ggaggaagat
     5281 gcatgtaaaa gcttcttgca aggggtaaag aaagtctgtg tgcccagtgc tgagaggcag
     5341 gtgagccaac agcaagtgca gggactaagg tgtataaaat gcctcctgtg gggcaggagg
     5401 ggagcagcat gctgagtgag agaggcagca ccctggaagc ttggaggagg agcaggattg
     5461 cttgtgctgt cagacaggtg tcaaacaggg ttccaggcag ctgctgagtg tcaggtgctt
     5521 cccataaatc atttcattga atttaatttt tccatctgcc ctgagagatg ggtattacca
     5581 ggggagaaac tgatattcaa acataggtcc tctgagcctg tgctcttccc acgatgccac
     5641 ctggtccacg tcataagact caacaatgca cctgttgtgc tgtgcacact tttcttcatt
     5701 tcatcctcgc agcttgcaag gttggtgtat tgtccccatt ttatttttat gtatttattt
     5761 atttattttg agatggagtg ttgcgcttgt tgcccaggct agagtgcaat ggcgcaatct
     5821 cagcttgctg caacctccac ctcccgggtt caagtgattc tcctgcctca gcctcccaag
     5881 tagctaggat tacaggcgcc tgccaccaca cccagctaat tttgtatttt taatagagat
     5941 gaggtttcat catgttggcc aggctggtct tgtacccctg acctcagatg atctacccgc
     6001 ctcagcctcc caaagtgctg gcattacagg tgtgagtcac tgcacccagc tgtccccatt
     6061 ttatagatga ggaactgagg ctcagagaag ctgaataatt tgcccatcct tgcggagaag
     6121 agctaaatgg aggagaggag gtagagctgc agggctttct gttcctgcca ggaatcatcc
     6181 agtagcccca ggccattctg accctagtgg gacaggagaa aaatcagtat caataagtca
     6241 ggcagcaagg ttccactgag catctccctg tgctagtgtg gagggagaca cccccctgca
     6301 acatcttcca tgactgccta ttgcccacaa gataaaattc agattcctca ggctggcatt
     6361 tgaggccctt catgatctgg ccccaactgc cccatccagc tgaatcatct gccatgtgct
     6421 cccgtgcagc agccccggca tcctcacctt tccagccacc ctgccttggc tcatgctgtt
     6481 ccctccaccc aggtgccctt catccccttc tctgtcatcc ctcaaaccct acctgttccc
     6541 tggagcctga attagccctg ctctcccctt ccacggtgga atccatctcc actgcaccct
     6601 ctcctccgaa tccctcctaa actgtgtgcc ccgctctgtc cccagcactt ctgaatgact
     6661 agctgtgtga gtaccagtct ccccacatgg actgggaggt ccttcaagga caggggcctc
     6721 ttccccgcca atacttctat ccccaggact tagcaaaagc aaggaacagt agtgctggtg
     6781 gaacagaatt gaaccgaacc gggtgcccac tctgaaggct catggcccag gggcatggac
     6841 tcctagccca gggctgagga gcaccagctt tggagtaaac agcgctgggt ttgagactag
     6901 gcttccctac tggccagctc tgtgactttg gccaggagac ttagcctctc tggccctcaa
     6961 tttccttatt aataaattga ggttagtgat atcgacccaa taggcatgtt gtgaagattg
     7021 gatgagatca tgcatattga gtgtagcatg tgcctagcac ctggtgagcc cccagtaaag
     7081 tcagttatca ttgttagtgt tgcctgcaga gatgatgtta caatgtgtag acatcaggtg
     7141 cccatgtgca cctgggtcgt tcaagcctac aagcactgtg tcatccaagc acatgtataa
     7201 ttcgagcttt gtatcgtcag tgcttccaag tgcctgtcaa tggacccagc tgggctggtg
     7261 gcatcagact ctcctgggaa actttaaaaa tctagtgtcc tagaaccatc ctcagaaaag
     7321 tctgggatgg cctgggtttt gcataagcaa gcctggtgat tcttaagtgc tgaagggctt
     7381 agttgtttgt ttttcttaaa tgagttatca aacattggat agctggtagg caacccagaa
     7441 gaggtgggag ttgcaagcca ggacttaaaa cagtagaatg aaggagtgag ggtatctcag
     7501 gcagagggca tggcataggc aaagccttgg gggaaggaag gaggaagaag gagactgtcc
     7561 tggctggagt ggtggaggac agggtcagag agggaggccg cagcttggcc atggggcttc
     7621 ttgaattcct gccacggagc tcagatttgt tctgatgggc aagggggatt aattgatgat
     7681 tcttgagcag aggagtgaca tgaaagaaac attcaggtca tatactaaca gatacaaaag
     7741 ggaaaggtgg cacgtattgg tggcaagaag gctggtttgt gccctgcggc agggatttca
     7801 ttctcaacct gccctgtgaa aggttttttc cactcatgtc actgggtgga tgccaggtca
     7861 ggactgaagc ttgcagagcc ggtgtcctgg attcctcctt gctgaccaca aatccagcag
     7921 agcagggcag ggaaccctgt ggcagaggct gttgcttccc gccaagacac cagggccctg
     7981 gccaaggcag tgctcagttc acagatgacc caggacaagg gcaaatgagg gccccagtga
     8041 ctcaggaagt ccccaggtca gaaaatgggc cccactggtg attctgcaga tagtccctgc
     8101 acatcccgga gaagagcaga cacggtgtgg tgggtaggtg gatgagacca ggctttgggg
     8161 atcagatcag caagcctggg ggtaaggagg gctagatttt atctagtcat gtgttcattc
     8221 atttgttcac ttattcattt attctaacca caaacactat gcacctgctt tgtgccaccc
     8281 cctgtgctag gtactggggg aagagacatg catctgaccc atgcttgtct tgggggaacc
     8341 tctactaccc cgggctaact gcagagtgaa cagtgacagg tcagtgctga gcagagggaa
     8401 gcacggcggg gatctgaccc cacctggaga gtcggcagga gcttctcaga gaggaactcc
     8461 caccaagcta tgtcctgaag gattcgctag ggaagagcag ggagggagaa cagcttgggc
     8521 gaagcctcca gccctgagcc accccaccac gttccaggat tgacctgggg taggtgtgaa
     8581 ctgagcttag aataggagcg gagggtggct ggagaagacg tgggtgagtg agtgactggg
     8641 gactggacca gggcgggctg tggttgttgc cctggagcac atggaaggcc ccagtgatgg
     8701 acaaggacaa agctacagtt tccatggaag gagggctttg cagtgctgag agctgcccaa
     8761 agaatgggta ggaacatgtc tgaaagaggt tacagttcct aacggagaga gagagacaga
     8821 tggagagaga gagacagaga gagagaacta gaaatcagac aggaaaagaa agggaattca
     8881 tccactggaa atatctgtga tcccacaaaa gaaacaaatg aaaacatggt taacctcagg
     8941 ggaatgcaaa ttaaaacaag aagctatgtt tcacccataa cactggcatc attttgtaaa
     9001 gatgatgtaa taaagataat aataggtgga gctggcagaa tgtggaggaa cagagctctt
     9061 tctgacgttg ctgatgtgaa tgggagtggt ggcagctttt ctgtgagtgc cttcacaggg
     9121 ggctgcccct tgattcagca attccacatg taggacttgc tcatttattc tagttttgtt
     9181 gttgttgttg ttgtttgttt gttgttttga gacaaggtct tgccgtattg cttaggctgg
     9241 tctcaaactc ctggatcaag tgatcctctg gcctcagcct cctgagtagc tgggattaca
     9301 ggagcacacc acagtgcctg gctttatcct gataatttac cctaaggaag aaatcagaca
     9361 actgtgctag actatactta cagaaggcag tttatcatag cgttgatgat ggtagatgaa
     9421 atgtggaaag aatctcaatg ttcagcaaca ggagactggc taagacatca tgccacagct
     9481 atgcaaggga cagatacacg gccattcaaa acagtgaccc tcctgattga atcaaatccc
     9541 ctatcaccac tctcacagca ccacagatct ctctcctttg tagcatttat taccatcata
     9601 attttacatt tctttgtgaa tttctttgat gaatttctat ctccttcatt ggacgctaag
     9661 ctctgtgaag gcaaggactc tattacgttc attattgtgc cctcagtgct tagttaagtt
     9721 cctggaacac agtatcctct caataaattg ttgttaaatg a
//
