LOCUS       NM_144695               4104 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens BRO1 domain and CAAX motif containing (BROX),
            transcript variant 1, mRNA.
ACCESSION   NM_144695
VERSION     NM_144695.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Wallis,S.S., Ventimiglia,L.N., Otigbah,E., Infante,E.,
            Cuesta-Geijo,M.A., Kidiyoor,G.R., Carbajal,M.A., Fleck,R.A.,
            Foiani,M., Garcia-Manyes,S., Martin-Serrano,J. and Agromayor,M.
  TITLE     The ESCRT machinery counteracts Nesprin-2G-mediated mechanical
            forces during nuclear envelope repair
  JOURNAL   Dev Cell 56 (23), 3192-3202 (2021)
   PUBMED   34818527
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 4104)
  AUTHORS   Pasquali,D., Torella,A., Accardo,G., Esposito,D., Del Vecchio
            Blanco,F., Salvatore,D., Sabatino,P., Pacini,F., Barbato,F.,
            Castagna,M.G., Cantara,S. and Nigro,V.
  TITLE     BROX haploinsufficiency in familial nonmedullary thyroid cancer
  JOURNAL   J Endocrinol Invest 44 (1), 165-171 (2021)
   PUBMED   32385852
  REMARK    GeneRIF: BROX haploinsufficiency in familial nonmedullary thyroid
            cancer.
REFERENCE   4  (bases 1 to 4104)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 4104)
  AUTHORS   Lee,J., Oh,K.J., Lee,D., Kim,B.Y., Choi,J.S., Ku,B. and Kim,S.J.
  TITLE     Structural Study of the HD-PTP Bro1 Domain in a Complex with the
            Core Region of STAM2, a Subunit of ESCRT-0
  JOURNAL   PLoS One 11 (2), e0149113 (2016)
   PUBMED   26866605
  REMARK    GeneRIF: homologous domain of human Bro1 domain-containing
            proteins, Alix and Brox, binds CHMP4B but not STAM2, despite their
            high structural similarity
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4104)
  AUTHORS   Zhai,Q., Landesman,M.B., Robinson,H., Sundquist,W.I. and Hill,C.P.
  TITLE     Structure of the Bro1 domain protein BROX and functional analyses
            of the ALIX Bro1 domain in HIV-1 budding
  JOURNAL   PLoS One 6 (12), e27466 (2011)
   PUBMED   22162750
  REMARK    GeneRIF: structural analysis of the Bro1 domain protein BROX and
            functional analyses of the ALIX Bro1 domain in HIV-1 budding
REFERENCE   7  (bases 1 to 4104)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   8  (bases 1 to 4104)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 4104)
  AUTHORS   Ichioka,F., Kobayashi,R., Katoh,K., Shibata,H. and Maki,M.
  TITLE     Brox, a novel farnesylated Bro1 domain-containing protein that
            associates with charged multivesicular body protein 4 (CHMP4)
  JOURNAL   FEBS J 275 (4), 682-692 (2008)
   PUBMED   18190528
  REMARK    GeneRIF: Results revealed that Strep-Brox(WT) exhibited
            accumulation in the perinuclear area suggested physiological
            significance of farnesylation of Brox in its subcellular
            distribution and efficient interaction with CHMP4s in vivo.
REFERENCE   10 (bases 1 to 4104)
  AUTHORS   Maurer-Stroh,S., Koranda,M., Benetka,W., Schneider,G., Sirota,F.L.
            and Eisenhaber,F.
  TITLE     Towards complete sets of farnesylated and geranylgeranylated
            proteins
  JOURNAL   PLoS Comput Biol 3 (4), e66 (2007)
   PUBMED   17411337
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL701416.1, DB504492.1, AK056983.1, BC113635.1 and AL392172.9.
            
            On Nov 23, 2018 this sequence version replaced NM_144695.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK056983.1, SRR1660805.166728.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340934.10/ ENSP00000343742.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AL701416.1         20-153
            135-214             DB504492.1         12-91               c
            215-252             AK056983.1         215-252
            253-1662            BC113635.1         1-1410
            1663-4104           AL392172.9         45063-47504
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q41"
     gene            1..4104
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /note="BRO1 domain and CAAX motif containing"
                     /db_xref="GeneID:148362"
                     /db_xref="HGNC:HGNC:26512"
     exon            1..379
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            380..496
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     CDS             396..1631
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /note="isoform a is encoded by transcript variant 1; BRO1
                     domain-containing protein BROX"
                     /codon_start=1
                     /product="BRO1 domain-containing protein BROX isoform a"
                     /protein_id="NP_653296.2"
                     /db_xref="CCDS:CCDS1534.1"
                     /db_xref="GeneID:148362"
                     /db_xref="HGNC:HGNC:26512"
                     /translation="MTHWFHRNPLKATAPVSFNYYGVVTGPSASKICNDLRSSRARLL
                     ELFTDLSCNPEMMKNAADSYFSLLQGFINSLDESTQESKLRYIQNFKWTDTLQGQVPS
                     AQQDAVFELISMGFNVALWYTKYASRLAGKENITEDEAKEVHRSLKIAAGIFKHLKES
                     HLPKLITPAEKGRDLESRLIEAYVIQCQAEAQEVTIARAIELKHAPGLIAALAYETAN
                     FYQKADHTLSSLEPAYSAKWRKYLHLKMCFYTAYAYCYHGETLLASDKCGEAIRSLQE
                     AEKLYAKAEALCKEYGETKGPGPTVKPSGHLFFRKLGNLVKNTLEKCQRENGFIYFQK
                     IPTEAPQLELKANYGLVEPIPFEFPPTSVQWTPETLAAFDLTKRPKDDSTKPKPEEEV
                     KPVKEPDIKPQKDTGCYIS"
     exon            497..603
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            604..700
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            701..796
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            797..869
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            870..975
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1065
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1151
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1233
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1384
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1544
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..4104
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4082..4087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /note="hexamer: AATAAA"
     polyA_site      4104
                     /gene="BROX"
                     /gene_synonym="C1orf58"
                     /note="major polyA site"
ORIGIN      
        1 actgcgccga gggccgccat cgctattgcg gcattctccc tcggctccgg aggtaggggc
       61 aactcttctc ttcctgtctg ggaaaaagac catagctcaa aaggaaggcc gagggagaag
      121 ttagaaaggg atgatgaacg aagcgttttg aggggactgc aacgccgcgg caatacccgc
      181 ccctgagctg cgcgcactac cgcctcggta gctatcatgg ccgccgggtc acgtgactcc
      241 ggcttggcgc cgtcctggtt ttccgtcacc ctggttctgt agtctcggtt ctccgactcc
      301 ctctttttct cgcttgtgga ctccgatata ttgcccttct tcccttagaa gaactgctga
      361 accgactctg agaaatttga aaacatctgg agaaaatgac ccattggttt cataggaacc
      421 cattaaaagc cacagctcct gtgtctttta attactatgg tgtagtcact ggcccttctg
      481 cttcaaaaat atgcaatgac ttgaggtcat ccagggcacg actccttgaa ctgttcactg
      541 atttgagctg taatccagaa atgatgaaga atgcagcaga ttcatatttc tcacttttac
      601 aaggtttcat aaattctttg gatgaatcta cccaagaaag caagttacga tatattcaaa
      661 atttcaagtg gactgataca ttgcaaggac aggttccaag tgcccagcag gatgctgttt
      721 ttgaattaat ttccatggga tttaatgtag ctttatggta taccaaatat gcttcaagac
      781 tggctggaaa agaaaatata acagaagatg aagcaaaaga agttcatcga agcctaaaga
      841 ttgcagctgg gatttttaaa catttaaagg aaagtcatct cccaaaactc attacacctg
      901 cggaaaaagg aagagattta gagtcacgac tcatagaagc atacgttatt caatgtcagg
      961 ctgaagctca agaagtaaca attgctcgag caattgaact aaaacatgct cctggactaa
     1021 ttgctgcact ggcgtatgaa acagccaatt tctatcaaaa agctgatcat actttatcca
     1081 gtttggagcc tgcatattct gccaaatgga gaaaatatct tcacttgaag atgtgtttct
     1141 acacagctta tgcttactgt taccatggtg agactttatt ggctagtgat aaatgcggtg
     1201 aagcaatcag gtctctccaa gaagcagaaa aattgtatgc aaaggcagaa gcactgtgta
     1261 aagaatatgg agaaaccaaa ggacctggac caacagtcaa accttcagga catctgttct
     1321 ttaggaaact tggaaacctt gtgaagaaca ccctagaaaa atgtcagaga gaaaatggat
     1381 ttatttactt tcaaaaaatt ccaacagaag ccccacagct ggaactcaaa gcaaattatg
     1441 gtctcgtaga gcctatacct ttcgaatttc ctcctacaag tgttcagtgg acaccagaaa
     1501 cattggctgc atttgatctc accaaaagac ccaaggatga cagtactaaa cccaaaccag
     1561 aagaagaagt gaaacctgtg aaagaaccag acatcaaacc tcaaaaggac actgggtgct
     1621 acatctccta aaatacaact tgcacttaga atttctctag cagtaaataa gataaaccac
     1681 agaatttcag ttcttatttc tcaaaatgat ttctctgaag cttgtagaat aactattata
     1741 ttcagagggt tatctgcctt cagcttactt gttcttaatt tttaatacag cggagatgtt
     1801 tcttgctttg ttttcagact ctccttttta atcaggacaa catttgaaag attttattgt
     1861 gcctctaaag ggtatatttg ggggttgggt ctttttgtta aactctggat agtaataagt
     1921 ttcaggtcag tataggcccg aatcatgtgg tttcaggtat gtttttcttt tttctttttc
     1981 tttttttttt ttcttttttt tttttttttt ttttgaggtg gagtctccct ctgttgccca
     2041 ggctggagtg cagtggcatg atctcggctc actgcaaccc tccacctcct gaattaaagt
     2101 gattctcctg cctcagcctc ccgagtagct gggattacag gcacgcacca ctacacccag
     2161 ataatttttg tatttttagt agagacgtga tttcaccatg ttggccaggc tggtctcgaa
     2221 ctcctgacct cagctgatct gcccgccttg gcctcccaaa gtgctgggat tacaggcgtg
     2281 atccactgca cctggcctca ggtatgtttt tctatctaca cacaaaaagt ttattttcct
     2341 tcctcttatt tgatatcaga agaatgacag gaatcagttt ttaacagaga tagtttttcc
     2401 gtttctccat tgtgttaatc agagcgcaga cttacattct cagtcattta tcttttctgg
     2461 tggagaataa aaagtcactt atagttaaag atcagaaaat tatcacacca aatccattac
     2521 atatgttaga atcaataatt tactcaaaaa tcagctttta taggtatgtt ggcaccttat
     2581 ccaagaactt atccctgttc cagtacccct gtgcttcatt cagtatcagt aaccctgcaa
     2641 tgatttttac aaatatcttt ttctagtggg ttttttactt agaggaaaga actttgtaat
     2701 agctcttaat gtttatatat aagagaagac agaatggaaa atgttttttg aagtcaaata
     2761 ttgcatgatg taaagaaaaa actttaaact taaatgagta ggttgtcctg aattacactg
     2821 gtaactctct acttctttat taaagaagtt atagtaagat gcctttgtta cctgatttca
     2881 gtgtacattg tttttatgtg gttttctgat aatgtcctgt cagaattgta ttggtttagg
     2941 ccaaaggcca tagcaaaaac atagagaaca aatatgatct taaaaataga ttgtaacatt
     3001 ttaaacaatt agtttagtaa tttgggaaat agtggatgca taatacttta acagaagtca
     3061 cacatctgta ggtttatctt ttgattattc tcctgaggca ttagatagtt tttctattat
     3121 aagtgacaga ttgactcact taatataaat aagtagcata cactaaaagc ttactatgtg
     3181 ccagatactg tgctggacac ttacatagat aagccattga atccacacag cagccctgta
     3241 agaaataagt aaaatctgca taaagtgttg aaataggaat atacatttca ttatgaaaaa
     3301 tgaatagatt atgcctatct gaatcaatat tgcattcaaa taagtgatta cttaaatcat
     3361 atatcatagt ttacactcct taaaaaaagg tacataaatt cagtgaggtt tttttcttaa
     3421 catacagaag tactaaatac tgcttgcagt ataatattga tattggaagc tgcagtttcc
     3481 agaataagtg gagtaataac taaacagaca tttaatttta tttcaatatc tatggaaaaa
     3541 acacttatta aatctccctg tattttatgt tgtctttatt acagaatcac ttgtctgttt
     3601 gttgtgtgcc acttactgac aaaactttaa acagtacttg atgccagctc tctactctgt
     3661 ggctgtggga cctgtttctt ttaggtactt gtgcttatta atctacttac taaattttca
     3721 cattgacatt tttggggatg atactaccat atacgaaatg gaaaatgtaa tatgctcagt
     3781 gcttctgtaa aatgcagcaa tactggtatt actttacatc agtaggcatc tttgacatga
     3841 gcatataaat attttgttga ctcagcaaag gtgacacttt gtgactaaag tatcccatta
     3901 tatataatgt tttttgaaat gttggaaatt ttggggaatt atcaaatgta tagaagttgc
     3961 atgaaggtta tagagaggtg taactgtttg ttaactatta catggatttc atactaggca
     4021 gtgacaacta acatgttact tcaactaaaa gtgtataatg ggttgtcttt ttatttatga
     4081 aaataaaagt aattttactt acaa
//
