LOCUS       NM_018462               1150 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens BRICK1 subunit of SCAR/WAVE actin nucleating complex
            (BRK1), mRNA.
ACCESSION   NM_018462
VERSION     NM_018462.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 1150)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1150)
  AUTHORS   Sugis,E., Dauvillier,J., Leontjeva,A., Adler,P., Hindie,V.,
            Moncion,T., Collura,V., Daudin,R., Loe-Mie,Y., Herault,Y.,
            Lambert,J.C., Hermjakob,H., Pupko,T., Rain,J.C., Xenarios,I.,
            Vilo,J., Simonneau,M. and Peterson,H.
  TITLE     HENA, heterogeneous network-based data set for Alzheimer's disease
  JOURNAL   Sci Data 6 (1), 151 (2019)
   PUBMED   31413325
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1150)
  AUTHORS   Li,M., Ling,B., Xiao,T., Tan,J., An,N., Han,N., Guo,S., Cheng,S.
            and Zhang,K.
  TITLE     Sp1 transcriptionally regulates BRK1 expression in non-small cell
            lung cancer cells
  JOURNAL   Gene 542 (2), 134-140 (2014)
   PUBMED   24680773
  REMARK    GeneRIF: Sp1 transcriptionally regulates BRK1 expression in
            non-small cell lung cancer cells.
REFERENCE   6  (bases 1 to 1150)
  AUTHORS   Mayne,M., Moffatt,T., Kong,H., McLaren,P.J., Fowke,K.R.,
            Becker,K.G., Namaka,M., Schenck,A., Bardoni,B., Bernstein,C.N. and
            Melanson,M.
  TITLE     CYFIP2 is highly abundant in CD4+ cells from multiple sclerosis
            patients and is involved in T cell adhesion
  JOURNAL   Eur J Immunol 34 (4), 1217-1227 (2004)
   PUBMED   15048733
REFERENCE   7  (bases 1 to 1150)
  AUTHORS   Maranchie,J.K., Afonso,A., Albert,P.S., Kalyandrug,S.,
            Phillips,J.L., Zhou,S., Peterson,J., Ghadimi,B.M., Hurley,K.,
            Riss,J., Vasselli,J.R., Ried,T., Zbar,B., Choyke,P., Walther,M.M.,
            Klausner,R.D. and Linehan,W.M.
  TITLE     Solid renal tumor severity in von Hippel Lindau disease is related
            to germline deletion length and location
  JOURNAL   Hum Mutat 23 (1), 40-46 (2004)
   PUBMED   14695531
REFERENCE   8  (bases 1 to 1150)
  AUTHORS   Eden,S., Rohatgi,R., Podtelejnikov,A.V., Mann,M. and Kirschner,M.W.
  TITLE     Mechanism of regulation of WAVE1-induced actin nucleation by Rac1
            and Nck
  JOURNAL   Nature 418 (6899), 790-793 (2002)
   PUBMED   12181570
REFERENCE   9  (bases 1 to 1150)
  AUTHORS   Witke,W., Podtelejnikov,A.V., Di Nardo,A., Sutherland,J.D.,
            Gurniak,C.B., Dotti,C. and Mann,M.
  TITLE     In mouse brain profilin I and profilin II associate with regulators
            of the endocytic pathway and actin assembly
  JOURNAL   EMBO J 17 (4), 967-976 (1998)
   PUBMED   9463375
REFERENCE   10 (bases 1 to 1150)
  AUTHORS   Kitamura,T., Kitamura,Y., Yonezawa,K., Totty,N.F., Gout,I.,
            Hara,K., Waterfield,M.D., Sakaue,M., Ogawa,W. and Kasuga,M.
  TITLE     Molecular cloning of p125Nap1, a protein that associates with an
            SH3 domain of Nck
  JOURNAL   Biochem Biophys Res Commun 219 (2), 509-514 (1996)
   PUBMED   8605018
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY148219.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018462.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY148219.1, SRR5189652.42369.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000530758.2/ ENSP00000432472.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1150              AY148219.1         27-1176
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..1150
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /note="BRICK1 subunit of SCAR/WAVE actin nucleating
                     complex"
                     /db_xref="GeneID:55845"
                     /db_xref="HGNC:HGNC:23057"
                     /db_xref="MIM:611183"
     exon            1..145
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..255
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /note="haematopoietic stem/progenitor cell protein 300;
                     BRICK1, SCAR/WAVE actin nucleating complex subunit"
                     /codon_start=1
                     /product="protein BRICK1"
                     /protein_id="NP_060932.2"
                     /db_xref="CCDS:CCDS54553.1"
                     /db_xref="GeneID:55845"
                     /db_xref="HGNC:HGNC:23057"
                     /db_xref="MIM:611183"
                     /translation="MAGQEDPVQREIHQDWANREYIEIITSSIKKIADFLNSFDMSCR
                     SRLATLNEKLTALERRIEYIEARVTKGETLT"
     exon            146..228
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /inference="alignment:Splign:2.1.0"
     exon            229..1150
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /inference="alignment:Splign:2.1.0"
     regulatory      859..864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /note="hexamer: AATAAA"
     polyA_site      877
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /note="major polyA site"
     regulatory      1125..1130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
                     /note="hexamer: AATAAA"
     polyA_site      1150
                     /gene="BRK1"
                     /gene_synonym="C3orf10; hHBrk1; HSPC300; MDS027"
ORIGIN      
        1 gcagtcgctc ttcctcaggc ggcggccatg gcgggacagg aggatccggt gcagcgggag
       61 attcaccagg actgggctaa ccgggagtac attgagataa tcaccagcag catcaagaaa
      121 atcgcagact ttctcaactc gttcgatatg tcttgtcgtt caagacttgc aacactaaac
      181 gagaaattga cagcccttga acggagaata gagtacattg aagctcgggt gacaaaaggt
      241 gagacactca cctagaacag tgccgtgctg ctgctgggaa gttgctttac acaacacagg
      301 ccacatggga aaggccccag cagccttcag ctccttcctt tctccttaaa gagcaacagg
      361 gcttattctt gtttttcttt tttcaaaagt gtggcctttg ggctctgcca tctggggtgt
      421 ggtgtggtat gtgggaagaa gttcagagga accgttggaa acgacgttag gcattttacc
      481 ttttcagtaa cattttatac atctacttgt caatgtattt gagacattca cagccaaaag
      541 cctgggactc tttgtgaagg tcctcctcac ctctatcttt ctttctctct ctctcaaact
      601 ttccttaaag ttctcattgc ctttgcactg cttctgtgaa cagtctttgt ctcctcccca
      661 cctttggtgg gaagtgcggg gcagtcctgg tcaagacact catgccctgg caatgtggct
      721 gccagagaat gttgttgcta acccaccagt ttcttgttga tttggagagg tcaaggccag
      781 gcccccactt ggcttgaagg gacattttca gacttttctt tctgtcactt ggagtgtcta
      841 tgcctctcat atttccctaa taaactcctc aactttttat ctgactgctg tgattatggt
      901 ggggagagga gctagagatg ggttcactta ttgcacagaa atgtaataca tggcgttatt
      961 attctaacat aaaactttca gatgtagctg tttgattcaa agcctaggtg gcttaccagc
     1021 ccaagtcccc atgtttggac tttcagctga ctagctcatc ttgggaatca tttggtcatt
     1081 cagcacattt accaagtatt tactatgtag gcatgttaaa ctccaataaa acatacagca
     1141 ttgaatcaga
//
