LOCUS       NM_018310               2560 bp    mRNA    linear   PRI 29-SEP-2024
DEFINITION  Homo sapiens BRF2 general transcription factor IIIB subunit (BRF2),
            mRNA.
ACCESSION   NM_018310
VERSION     NM_018310.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Sui,R., Liu,C., Wang,N., Fan,X., Han,S., Zhang,J., Hou,L., Zhang,X.
            and Xu,A.
  TITLE     TFIIB-related factor 2 regulates glucose-regulated protein 78
            expression in acquired middle ear cholesteatoma
  JOURNAL   Biochem Biophys Res Commun 540, 95-100 (2021)
   PUBMED   33453679
  REMARK    GeneRIF: TFIIB-related factor 2 regulates glucose-regulated protein
            78 expression in acquired middle ear cholesteatoma.
REFERENCE   3  (bases 1 to 2560)
  AUTHORS   Cabarcas-Petroski,S., Meneses,P.I. and Schramm,L.
  TITLE     A meta-analysis of BRF2 as a prognostic biomarker in invasive
            breast carcinoma
  JOURNAL   BMC Cancer 20 (1), 1093 (2020)
   PUBMED   33176745
  REMARK    GeneRIF: A meta-analysis of BRF2 as a prognostic biomarker in
            invasive breast carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2560)
  AUTHORS   Bian,Y., Li,Q., Li,Q. and Pan,R.
  TITLE     Silencing of BRF2 inhibits the growth and metastasis of lung cancer
            cells
  JOURNAL   Mol Med Rep 22 (3), 1767-1774 (2020)
   PUBMED   32705258
  REMARK    GeneRIF: Silencing of BRF2 inhibits the growth and metastasis of
            lung cancer cells.
REFERENCE   5  (bases 1 to 2560)
  AUTHORS   Yu,X., Zheng,H., Sun,R., Qian,X., Jiang,P., Yang,B., Liu,J. and
            Li,X.
  TITLE     MicroRNA-425-5p Inhibits Lung Cancer Cell Growth in Vitro and in
            Vivo by Downregulating TFIIB-Related Factor 2
  JOURNAL   Technol Cancer Res Treat 19, 1533033819901115 (2020)
   PUBMED   31964245
  REMARK    GeneRIF: MicroRNA-425-5p Inhibits Lung Cancer Cell Growth in Vitro
            and in Vivo by Downregulating TFIIB-Related Factor 2.
REFERENCE   6  (bases 1 to 2560)
  AUTHORS   Hinkley,C.S., Hirsch,H.A., Gu,L., LaMere,B. and Henry,R.W.
  TITLE     The small nuclear RNA-activating protein 190 Myb DNA binding domain
            stimulates TATA box-binding protein-TATA box recognition
  JOURNAL   J Biol Chem 278 (20), 18649-18657 (2003)
   PUBMED   12621023
REFERENCE   7  (bases 1 to 2560)
  AUTHORS   Cabart,P. and Murphy,S.
  TITLE     BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of
            polymerase III small nuclear RNA gene promoters through its
            interaction with TATA-binding protein
  JOURNAL   J Biol Chem 276 (46), 43056-43064 (2001)
   PUBMED   11564744
REFERENCE   8  (bases 1 to 2560)
  AUTHORS   Teichmann,M., Wang,Z. and Roeder,R.G.
  TITLE     A stable complex of a novel transcription factor IIB- related
            factor, human TFIIIB50, and associated proteins mediate selective
            transcription by RNA polymerase III of genes with upstream promoter
            elements
  JOURNAL   Proc Natl Acad Sci U S A 97 (26), 14200-14205 (2000)
   PUBMED   11121026
REFERENCE   9  (bases 1 to 2560)
  AUTHORS   Schramm,L., Pendergrast,P.S., Sun,Y. and Hernandez,N.
  TITLE     Different human TFIIIB activities direct RNA polymerase III
            transcription from TATA-containing and TATA-less promoters
  JOURNAL   Genes Dev 14 (20), 2650-2663 (2000)
   PUBMED   11040218
REFERENCE   10 (bases 1 to 2560)
  AUTHORS   Sutcliffe,J.E., Cairns,C.A., McLees,A., Allison,S.J., Tosh,K. and
            White,R.J.
  TITLE     RNA polymerase III transcription factor IIIB is a target for
            repression by pocket proteins p107 and p130
  JOURNAL   Mol Cell Biol 19 (6), 4255-4261 (1999)
   PUBMED   10330166
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF206673.2, AK001914.1,
            AA496239.1 and AC138356.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_018310.3.
            
            Summary: This gene encodes one of the multiple subunits of the RNA
            polymerase III transcription factor complex required for
            transcription of genes with promoter elements upstream of the
            initiation site. The product of this gene, a TFIIB-like factor, is
            directly recruited to the TATA-box of polymerase III small nuclear
            RNA gene promoters through its interaction with the TATA-binding
            protein. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7410570.427703.1,
                                           DRR138522.14564.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000220659.11/ ENSP00000220659.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1118              AF206673.2         53-1170
            1119-1941           AK001914.1         1133-1955
            1942-1948           AA496239.1         8-14                c
            1949-2560           AC138356.3         199-810
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.23"
     gene            1..2560
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="BRF2 general transcription factor IIIB subunit"
                     /db_xref="GeneID:55290"
                     /db_xref="HGNC:HGNC:17298"
                     /db_xref="MIM:607013"
     exon            1..232
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..1338
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="transcription factor IIB- related factor, TFIIIB50;
                     BRF2, RNA polymerase III transcription initiation factor
                     50 kDa subunit; RNA polymerase III transcription
                     initiation factor BRFU; BRF-2; hBRFU; BRF2, subunit of RNA
                     polymerase III transcription initiation factor, BRF1-like;
                     hTFIIIB50; BRF2 RNA polymerase III transcription
                     initiation factor subunit; B-related factor 2"
                     /codon_start=1
                     /product="transcription factor IIIB 50 kDa subunit"
                     /protein_id="NP_060780.2"
                     /db_xref="CCDS:CCDS6098.1"
                     /db_xref="GeneID:55290"
                     /db_xref="HGNC:HGNC:17298"
                     /db_xref="MIM:607013"
                     /translation="MPGRGRCPDCGSTELVEDSHYSQSQLVCSDCGCVVTEGVLTTTF
                     SDEGNLREVTYSRSTGENEQVSRSQQRGLRRVRDLCRVLQLPPTFEDTAVAYYQQAYR
                     HSGIRAARLQKKEVLVGCCVLITCRQHNWPLTMGAICTLLYADLDVFSSTYMQIVKLL
                     GLDVPSLCLAELVKTYCSSFKLFQASPSVPAKYVEDKEKMLSRTMQLVELANETWLVT
                     GRHPLPVITAATFLAWQSLQPADRLSCSLARFCKLANVDLPYPASSRLQELLAVLLRM
                     AEQLAWLRVLRLDKRSVVKHIGDLLQHRQSLVRSAFRDGTAEVETREKEPPGWGQGQG
                     EGEVGNNSLGLPQGKRPASPALLLPPCMLKSPKRICPVPPVSTVTGDENISDSEIEQY
                     LRTPQEVRDFQRAQAARQAATSVPNPP"
     exon            233..292
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /inference="alignment:Splign:2.1.0"
     exon            293..614
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /inference="alignment:Splign:2.1.0"
     exon            615..2560
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1891..1896
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="hexamer: AACAAG"
     polyA_site      1915
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
     regulatory      1924..1929
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="hexamer: TATAAA"
     polyA_site      1946
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="major polyA site"
     regulatory      2066..2071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="hexamer: AATACA"
     polyA_site      2092
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
     regulatory      2110..2115
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
                     /note="hexamer: CATAAA"
     polyA_site      2136
                     /gene="BRF2"
                     /gene_synonym="BRFU; TFIIIB50"
ORIGIN      
        1 acttcttgct gcctcacggt tgctgttgca gacccaggag tctctgaagg cttccgcggc
       61 tttggggccg gttgtgagat gccaggcaga ggccgctgcc cggactgcgg ctccacggag
      121 ctggtggaag actcgcacta ttcgcagagc cagctggtgt gctccgactg cggctgcgtg
      181 gtcaccgagg gggtccttac cactaccttc agcgacgagg gcaatctccg agaggtaaca
      241 tattcccgaa gcacagggga aaacgaacaa gttagtcgca gccagcaacg aggtctccgg
      301 cgagtgagag acctttgtcg agttctgcag ttgccaccaa catttgagga taccgcggtt
      361 gcctactacc aacaggcata tcggcactct ggcatccgag cggccaggct gcaaaagaag
      421 gaggtgttgg ttgggtgctg cgtcttaatc acctgccgac agcataactg gcccctaaca
      481 atgggggcca tctgcacgct gttgtatgca gatttggatg tgttttctag cacttacatg
      541 cagatagtga agctcctggg actggatgtg ccatctctgt gcttggcaga actggtgaag
      601 acctattgca gcagcttcaa actgttccaa gcttcacctt ctgtgccagc caaatacgtg
      661 gaagacaaag agaagatgct gtctcgaaca atgcagttgg tggagctggc aaatgagacg
      721 tggctggtga ccgggaggca tcccttgccc gtcatcactg ctgcgacttt cctggcttgg
      781 cagtcgctgc agcctgcaga tcggctttca tgttcccttg cccgattttg taaattggca
      841 aatgtggacc tgccctaccc ggcgtcctcc cgcctgcagg agctgctggc tgtgctgctg
      901 cggatggctg agcagctggc ctggttacga gttctgagac ttgacaaacg gtctgtggtg
      961 aagcacatcg gtgaccttct ccagcaccgc cagtcactgg tccgctctgc ctttcgggat
     1021 gggacagcag aagtggagac ccgagagaag gagccaccgg ggtggggaca ggggcaagga
     1081 gaaggggagg tgggaaataa ttccttaggt ttaccccagg ggaagcggcc ggccagtcct
     1141 gcccttctct tgccaccctg catgttgaag tccccgaagc ggatctgccc tgtaccccct
     1201 gtctccactg tcactggaga tgagaacatt tctgatagtg aaatagaaca gtatttgcgt
     1261 acccctcagg aagttaggga ctttcagaga gcccaggctg ctagacaggc tgccacgagt
     1321 gtccctaacc ctccctgatg gatatccact gggagcactt catcctgttc tgacagcttg
     1381 ataacattcc tgttataacc aaggatggaa gtgtacacca gtccgtaggt attgcttttc
     1441 ttgtttgaag gaaccaagag gggctctgcc attagttgga ccctgggtcc tggagtaaag
     1501 tcaggagtgc agggatgact ataggtagga gagattccca tcccttggtg tgggagagca
     1561 agttgcctat gtccatgttc tgtgagatgg ctttcctcat agatggatgg gaaaatgtca
     1621 ggctctttgc tgctggtttg aattggacac actgctgcgg ctcctcctgc aggcctgagg
     1681 gggcttccct ctgcttgtgg agtggttggc attcccagca gtatcaaccc tcagaggagc
     1741 gggaactggg gaattctggc cctacgtgca ttcacaggca atgatgggtt tgtgtgtatg
     1801 gtgtcatgag atcctctacc tcataacaaa aggacagtgg gtagactaag gcagtagctc
     1861 aaagggcttt gcaaaatttt aatatattaa aacaagaggc atctgctaga aaacattcta
     1921 ttgtataaaa cccgagttct taaaaacatg ttttctttgg cactttcatt ccctccctcc
     1981 cctttcccca gcatattgca aaaagctctc cagtgctaag gcattggcag ggtgtgtaaa
     2041 cagcagccag catatgtgga agaataatac aaagcttttt tttttcttct aatatgtctg
     2101 tgcagcaagc ataaataaca ggacccattc caaggagtgt gtgtgggttt tccccctccc
     2161 ctgtgtcctc tgtcaccttg gtgatgaggc cagagtgatg tgaagactgg gagggaaccc
     2221 taggtcagac cttggtttcc tgttgttctt cccaagaccc agggttccct cggtttccat
     2281 ttggtcctgt agtgaaatgc tggtggttgg ttttaggttg gggaaaagac gggtgctggt
     2341 gtttaaaagg agtaaaggca taggcccctc atgacctgca gggaccccag ccactgcctc
     2401 tgggcttggc ttgagctcct gggagtcggg aagctggttt gacattttgt aggtttctct
     2461 ctactgtgga gctggagatc aaatgattgg gatggttctt cctctggcct tacggaggca
     2521 aggaagtgct cttccttagt cccgtggtgt tgtaaggctg
//
