LOCUS       NM_001519               3671 bp    mRNA    linear   PRI 29-SEP-2024
DEFINITION  Homo sapiens BRF1 general transcription factor IIIB subunit (BRF1),
            transcript variant 1, mRNA.
ACCESSION   NM_001519
VERSION     NM_001519.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Wang,J., Chen,Q., Wang,X., Zhao,S., Deng,H., Guo,B., Zhang,C.,
            Song,X., Deng,W., Zhang,T. and Ni,H.
  TITLE     TFIIB-related factor 1 is a nucleolar protein that promotes RNA
            polymerase I-directed transcription and tumour cell growth
  JOURNAL   Hum Mol Genet 32 (1), 104-121 (2023)
   PUBMED   35925837
  REMARK    GeneRIF: TFIIB-related factor 1 is a nucleolar protein that
            promotes RNA polymerase I-directed transcription and tumour cell
            growth.
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Zhao,X., Lan,Y., Miao,J., Li,G., Sun,W., Qiu,X., Zhu,S. and Zhu,Z.
  TITLE     A novel BRF1 mutation in two middle-aged siblings with
            cerebellofaciodental syndrome
  JOURNAL   Chin Med J (Engl) 135 (19), 2375-2377 (2022)
   PUBMED   34935685
  REMARK    GeneRIF: A novel BRF1 mutation in two middle-aged siblings with
            cerebellofaciodental syndrome.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3671)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 3671)
  AUTHORS   Guillon,J., Coquelet,H., Leman,G., Toutain,B., Petit,C., Henry,C.,
            Boissard,A., Guette,C. and Coqueret,O.
  TITLE     tRNA biogenesis and specific aminoacyl-tRNA synthetases regulate
            senescence stability under the control of mTOR
  JOURNAL   PLoS Genet 17 (12), e1009953 (2021)
   PUBMED   34928935
  REMARK    GeneRIF: tRNA biogenesis and specific aminoacyl-tRNA synthetases
            regulate senescence stability under the control of mTOR.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3671)
  AUTHORS   Anuraga,G., Tang,W.C., Phan,N.N., Ta,H.D.K., Liu,Y.H., Wu,Y.F.,
            Lee,K.H. and Wang,C.Y.
  TITLE     Comprehensive Analysis of Prognostic and Genetic Signatures for
            General Transcription Factor III (GTF3) in Clinical Colorectal
            Cancer Patients Using Bioinformatics Approaches
  JOURNAL   Curr Issues Mol Biol 43 (1), 2-20 (2021)
   PUBMED   33925358
  REMARK    GeneRIF: Comprehensive Analysis of Prognostic and Genetic
            Signatures for General Transcription Factor III (GTF3) in Clinical
            Colorectal Cancer Patients Using Bioinformatics Approaches.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3671)
  AUTHORS   Chu,W.M., Wang,Z., Roeder,R.G. and Schmid,C.W.
  TITLE     RNA polymerase III transcription repressed by Rb through its
            interactions with TFIIIB and TFIIIC2
  JOURNAL   J Biol Chem 272 (23), 14755-14761 (1997)
   PUBMED   9169441
REFERENCE   7  (bases 1 to 3671)
  AUTHORS   Wang,Z. and Roeder,R.G.
  TITLE     Three human RNA polymerase III-specific subunits form a subcomplex
            with a selective function in specific transcription initiation
  JOURNAL   Genes Dev 11 (10), 1315-1326 (1997)
   PUBMED   9171375
REFERENCE   8  (bases 1 to 3671)
  AUTHORS   Chesnokov,I., Chu,W.M., Botchan,M.R. and Schmid,C.W.
  TITLE     p53 inhibits RNA polymerase III-directed transcription in a
            promoter-dependent manner
  JOURNAL   Mol Cell Biol 16 (12), 7084-7088 (1996)
   PUBMED   8943363
REFERENCE   9  (bases 1 to 3671)
  AUTHORS   Mital,R., Kobayashi,R. and Hernandez,N.
  TITLE     RNA polymerase III transcription from the human U6 and adenovirus
            type 2 VAI promoters has different requirements for human BRF, a
            subunit of human TFIIIB
  JOURNAL   Mol Cell Biol 16 (12), 7031-7042 (1996)
   PUBMED   8943358
REFERENCE   10 (bases 1 to 3671)
  AUTHORS   Wang,Z. and Roeder,R.G.
  TITLE     Structure and function of a human transcription factor TFIIIB
            subunit that is evolutionarily conserved and contains both TFIIB-
            and high-mobility-group protein 2-related domains
  JOURNAL   Proc Natl Acad Sci U S A 92 (15), 7026-7030 (1995)
   PUBMED   7624363
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL512355.6, BX440529.2,
            BC016743.2, U75276.1 and BC071637.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_001519.3.
            
            Summary: This gene encodes one of the three subunits of the RNA
            polymerase III transcription factor complex. This complex plays a
            central role in transcription initiation by RNA polymerase III on
            genes encoding tRNA, 5S rRNA, and other small structural RNAs. The
            gene product belongs to the TF2B family. Several alternatively
            spliced variants encoding different isoforms, that function at
            different promoters transcribed by RNA polymerase III, have been
            identified. [provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U28838.1, U75276.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000547530.7/ ENSP00000448387.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 AL512355.6         160433-160441       c
            10-168              BX440529.2         286-444
            169-356             BC016743.2         106-293
            357-3125            U75276.1           52-2820
            3126-3671           BC071637.1         2181-2726
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.33"
     gene            1..3671
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /note="BRF1 general transcription factor IIIB subunit"
                     /db_xref="GeneID:2972"
                     /db_xref="HGNC:HGNC:11551"
                     /db_xref="MIM:604902"
     exon            1..556
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     CDS             373..2406
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /note="isoform 1 is encoded by transcript variant 1; TATA
                     box binding protein (TBP)-associated factor, RNA
                     polymerase III, GTF3B subunit 2; TBP - associated factor,
                     RNA polymerase III, 90kD; BRF1 RNA polymerase III
                     transcription initiation factor subunit; B - related
                     factor 1; TATA box binding protein (TBP)-associated factor
                     3C; general transcription factor IIIB, 90kD subunit;
                     transcription factor IIIB 90 kDa subunit; BRF1, RNA
                     polymerase III transcription initiation factor 90 kDa
                     subunit; BRF1 homolog, subunit of RNA polymerase III
                     transcription initiation factor IIIB; epididymis secretory
                     sperm binding protein Li 76p"
                     /codon_start=1
                     /product="transcription factor IIIB 90 kDa subunit isoform
                     1"
                     /protein_id="NP_001510.2"
                     /db_xref="CCDS:CCDS10001.1"
                     /db_xref="GeneID:2972"
                     /db_xref="HGNC:HGNC:11551"
                     /db_xref="MIM:604902"
                     /translation="MTGRVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQFV
                     ESSGGGSSAVGQFVSLDGAGKTPTLGGGFHVNLGKESRAQTLQNGRRHIHHLGNQLQL
                     NQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNV
                     YVLGKTFLLLARELCINAPAIDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKRDW
                     MHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLT
                     IDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELEN
                     SRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGS
                     SEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLS
                     GIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKK
                     SCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSAS
                     ARKLSRRRTPASRSGADPVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARP
                     QAVLVESGPVSYHADEEADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY"
     exon            557..637
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            638..811
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            812..843
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            844..916
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1066
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1160
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1287
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1327
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1420
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1687
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1749
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1831
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1887
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2144
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2196
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2368
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     exon            2369..3671
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3636..3641
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /note="hexamer: AGTAAA"
     polyA_site      3671
                     /gene="BRF1"
                     /gene_synonym="BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p;
                     TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90"
                     /note="major polyA site"
ORIGIN      
        1 gccagtcctc ggctgcgctc accggtaggc cccgctcggg ttccgccgaa gcccagcccc
       61 cgcaggtcgc ccctccgacg ccggcgcgcc gcaagggagg ccagctcgct cgcagtgggg
      121 aggtcgcggc tccagtcctc gcgtccccgc cgtggtcccg gtgcctgtcc catcccgcgg
      181 gcgcggccgt tgcgggcggc gcggcccggg cgcgcgcggc gaatctgcgg ctgcgaatcg
      241 gctggagcgg ggcctcgcga gagcggcgag gcctgggcgg ctgggcctgg gcgggcggcg
      301 cgggctgctc cggaggctcg ggtggcttga gagtcttggg aggctccggg cgctgcccgc
      361 gcggtcgccg gcatgacggg ccgcgtgtgc cgcggttgcg gcggcacgga catcgagctg
      421 gacgcggcgc gcggggacgc ggtgtgcacc gcctgcggct cagtgctgga ggacaacatc
      481 atcgtgtccg aggtgcagtt cgtggagagc agcggcggcg gctcctcggc cgtgggccag
      541 ttcgtgtccc tggacggtgc tggcaaaacc ccgactctgg gtggcggctt ccacgtgaat
      601 ctggggaagg agtcgagagc gcagaccctg cagaatggga ggcgccacat ccaccacctg
      661 gggaaccagc tgcagctgaa ccagcactgc ctggacaccg ccttcaactt cttcaagatg
      721 gccgtgagca ggcacctgac ccgcggccgg aagatggccc acgtgattgc tgcctgcctc
      781 tacctggtct gccgtacgga gggcacgccg cacatgctcc tggacctcag cgacctgctc
      841 caggtgaatg tgtacgtgct tggaaagacg tttcttctct tggcaagaga gctctgcatc
      901 aatgcgccgg ccatagaccc gtgcctgtat attccacgct ttgcgcacct gctggaattc
      961 ggggagaaga accacgaggt gtccatgact gccctgaggc tcctacagag gatgaagcgg
     1021 gactggatgc acacaggccg gcgcccctcg ggcctctgcg gagcagcgct cctggttgca
     1081 gccagaatgc atgacttcag gaggactgtg aaggaggtca tcagtgtggt caaagtgtgt
     1141 gagtccacgc tgcggaagag gctcacggaa tttgaagaca cccccaccag tcagttgacc
     1201 attgatgagt tcatgaagat cgacctggag gaggagtgcg accccccctc gtacacagct
     1261 gggcagagga agctgcggat gaagcagctt gaacaagtcc tgtcaaaaaa actggaggag
     1321 gttgaaggtg aaatatccag ttaccaggat gcaattgaga ttgaactaga aaacagccgg
     1381 ccaaaggcca aggggggcct ggccagcctg gcaaaagatg gctccaccga ggacaccgcg
     1441 tccagcttgt gtggcgagga ggacacagag gacgaggagc tggaagccgc ggccagccac
     1501 ctgaacaaag acttataccg ggagctcctt ggtggtgccc ccggcagctc ggaagcagca
     1561 ggaagccccg agtggggcgg cagacctccg gccctggggt ccctgctgga ccccctcccc
     1621 actgcagcca gcctgggcat ctcagactcc atccgcgaat gcatctcctc tcagagcagc
     1681 gaccccaaag atgcttcagg agacggtgag ctggacctca gtggcattga tgacctggag
     1741 attgacaggt acatcctgaa tgagtcggaa gcccgcgtga aggccgagct gtggatgagg
     1801 gagaacgccg agtacctgcg ggaacagagg gaaaaagaag caagaatagc gaaagagaag
     1861 gagctcggca tctacaagga acacaagccc aagaagtctt gcaagcgacg ggagccaatt
     1921 caggccagta ccgccaggga ggccatcgag aagatgctgg agcagaagaa gatctccagc
     1981 aagatcaatt atagcgtgct ccggggcctc agcagcgccg gcgggggcag tccgcacagg
     2041 gaggatgcac agcccgagca tagcgccagt gccaggaagc tgtcacgaag gaggacgccg
     2101 gccagcagaa gtggggctga ccctgtgacc agtgtgggga aaaggttgag gcctctggtg
     2161 tctacgcagc cagcaaagaa ggtggccacg ggagaggctt tgctcccaag ctctcccacc
     2221 ctcggagctg agcctgccag gccccaggcg gtgctggtgg agagcgggcc cgtgtcatac
     2281 cacgccgacg aggaggctga cgaggaggag cctgacgagg aggacgggga gccctgcgtc
     2341 agtgccctgc agatgatggg cagcaacgac tatggctgtg atggcgatga ggacgacggc
     2401 tactgaagtg tggcctccag gcaggtgatg tcctggcagg gggcctcgcg ggtctcctca
     2461 gcatcagacg ggcttccagg accgcagcag gcaggcccca gcgccgagac tcctggtgac
     2521 aggtggcacc tgtcccacag ccctcgtccc atgtggaact taccattggg attgtgtttc
     2581 tattcagcaa gggaaaccgg accaagcgtc tgcatgtgtg tgatcagatg tgggccgggt
     2641 gtgtgcaggg ctgggtcccg ctgcctgccg tcgactcatc caaggaccct ccaaggctgg
     2701 cagtgtggtg ttgctactat taaggaaaca ggcttggggc agccccactg ctggtccaag
     2761 tgtgtggagg gctgagtgtg ctggccctgt gactcaggac cagctctgga gtctccagcc
     2821 caccctccgc accgtcccct cctgagcagc actcggcgcc agcagcctct gccagagtgg
     2881 aagccagagc cctgcaggtg tccggcgcag ccgtgggagc tgaggatctg gcacttgaga
     2941 ggcagcagct ccttgaaggt cctctgcctc cagctgtggc cctgcatcca gatacctgcc
     3001 tcgtccgagg cagacacccc cacccctgcc tcctccagac ccccctcccc gctgcctgca
     3061 ccgcctggag cagcatgggg gtcagacccc tgctccaggg ccacttgagt tgtgggccca
     3121 ggagccctgc ggctgccggc aggtgaactg agtgcccgac agctgagacc ggcgcccacc
     3181 cgtcctgagc atagctctgt aggcagtgcg ggcatagcct gcatagtgtc ctggcgctgg
     3241 gagttccccg tggacagagc cagagggcag tggcgctccc tgtcagagct ggatcaggcc
     3301 ccccatcgag gagggagggc agacggaggc ccgagagcct ccccaggcct cttcgtggga
     3361 aggccccagt accactcgta ggaggtctca gctctggcat ggctgccccg gatgtggccg
     3421 agggggcttc accctgtgtc cttaggaggg ggtggccttg aggcagagcc gtgcctcact
     3481 gacccccagg ggcctcatcc tccccatgga atgggctgta tgtcctgccc caacttggcc
     3541 cgcagcaggc cagacccccc tacccccgcc cagagctcag tagccagcct gggtcctgcc
     3601 agggcttctc gagggcttgg gggaagaata gatttagtaa agcaggaaga tctgttgtta
     3661 cttaacagaa a
//
