LOCUS       NM_207189               3310 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens bromodomain testis associated (BRDT), transcript
            variant 1, mRNA.
ACCESSION   NM_207189
VERSION     NM_207189.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Wang,X., Kutschat,A.P., Yamada,M., Prokakis,E., Bottcher,P.,
            Tanaka,K., Doki,Y., Hamdan,F.H. and Johnsen,S.A.
  TITLE     Bromodomain protein BRDT directs DeltaNp63 function and
            super-enhancer activity in a subset of esophageal squamous cell
            carcinomas
  JOURNAL   Cell Death Differ 28 (7), 2207-2220 (2021)
   PUBMED   33658703
  REMARK    GeneRIF: Bromodomain protein BRDT directs DeltaNp63 function and
            super-enhancer activity in a subset of esophageal squamous cell
            carcinomas.
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Yu,Z., Ku,A.F., Anglin,J.L., Sharma,R., Ucisik,M.N., Faver,J.C.,
            Li,F., Nyshadham,P., Simmons,N., Sharma,K.L., Nagarajan,S.,
            Riehle,K., Kaur,G., Sankaran,B., Storl-Desmond,M., Palmer,S.S.,
            Young,D.W., Kim,C. and Matzuk,M.M.
  TITLE     Discovery and characterization of bromodomain 2-specific inhibitors
            of BRDT
  JOURNAL   Proc Natl Acad Sci U S A 118 (9) (2021)
   PUBMED   33637650
  REMARK    GeneRIF: Discovery and characterization of bromodomain 2-specific
            inhibitors of BRDT.
REFERENCE   3  (bases 1 to 3310)
  AUTHORS   Wan,P., Chen,Z., Zhong,W., Jiang,H., Huang,Z., Peng,D., He,Q. and
            Chen,N.
  TITLE     BRDT is a novel regulator of eIF4EBP1 in renal cell carcinoma
  JOURNAL   Oncol Rep 44 (6), 2475-2486 (2020)
   PUBMED   33125143
  REMARK    GeneRIF: BRDT is a novel regulator of eIF4EBP1 in renal cell
            carcinoma.
REFERENCE   4  (bases 1 to 3310)
  AUTHORS   Wang,B., Fan,P., Zhao,J., Wu,H., Jin,X. and Wu,H.
  TITLE     FBP1 loss contributes to BET inhibitors resistance by undermining
            c-Myc expression in pancreatic ductal adenocarcinoma
  JOURNAL   J Exp Clin Cancer Res 37 (1), 224 (2018)
   PUBMED   30201002
  REMARK    GeneRIF: FBP1 modulates the sensitivity of pancreatic cancer cells
            to BET inhibitors by decreasing the expression of c-Myc. These
            findings highlight FBP1 could be used as a therapeutic niche for
            patient-tailored therapies
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3310)
  AUTHORS   Olp,M.D., Zhu,N. and Smith,B.C.
  TITLE     Metabolically Derived Lysine Acylations and Neighboring
            Modifications Tune the Binding of the BET Bromodomains to Histone
            H4
  JOURNAL   Biochemistry 56 (41), 5485-5495 (2017)
   PUBMED   28945351
  REMARK    GeneRIF: The bromodomain and extraterminal domain (BET) family
            consists of BRDT, BRD2, BRD3, and BRD4, each containing 2
            bromodomains located N-terminal to extraterminal domain, which is
            located near C-terminus. Data suggest that 10 distinct acylations
            participate in binding of BET family proteins to histone 4
            (oligopeptide fragments used here); C-terminal bromodomains do not
            cooperatively bind multiple acylation sites.
REFERENCE   6  (bases 1 to 3310)
  AUTHORS   Zheng,Y., Yuan,W., Zhou,Z., Xu,M. and Sha,J.H.
  TITLE     Molecular cloning and expression of a novel alternative splice
            variant of BRDT gene
  JOURNAL   Int J Mol Med 15 (2), 315-321 (2005)
   PUBMED   15647849
  REMARK    GeneRIF: BRDT-NY gene is an alternatively spliced variant that may
            have an important role in the process of spermatogenesis and may be
            correlated with male infertility
REFERENCE   7  (bases 1 to 3310)
  AUTHORS   Pivot-Pajot,C., Caron,C., Govin,J., Vion,A., Rousseaux,S. and
            Khochbin,S.
  TITLE     Acetylation-dependent chromatin reorganization by BRDT, a
            testis-specific bromodomain-containing protein
  JOURNAL   Mol Cell Biol 23 (15), 5354-5365 (2003)
   PUBMED   12861021
REFERENCE   8  (bases 1 to 3310)
  AUTHORS   Scanlan,M.J., Altorki,N.K., Gure,A.O., Williamson,B., Jungbluth,A.,
            Chen,Y.T. and Old,L.J.
  TITLE     Expression of cancer-testis antigens in lung cancer: definition of
            bromodomain testis-specific gene (BRDT) as a new CT gene, CT9
  JOURNAL   Cancer Lett 150 (2), 155-164 (2000)
   PUBMED   10704737
REFERENCE   9  (bases 1 to 3310)
  AUTHORS   Dhalluin,C., Carlson,J.E., Zeng,L., He,C., Aggarwal,A.K. and
            Zhou,M.M.
  TITLE     Structure and ligand of a histone acetyltransferase bromodomain
  JOURNAL   Nature 399 (6735), 491-496 (1999)
   PUBMED   10365964
REFERENCE   10 (bases 1 to 3310)
  AUTHORS   Jones,M.H., Numata,M. and Shimane,M.
  TITLE     Identification and characterization of BRDT: A testis-specific gene
            related to the bromodomain genes RING3 and Drosophila fsh
  JOURNAL   Genomics 45 (3), 529-534 (1997)
   PUBMED   9367677
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114486.2.
            
            On Aug 5, 2020 this sequence version replaced NM_207189.3.
            
            Summary: BRDT is similar to the RING3 protein family. It possesses
            2 bromodomain motifs and a PEST sequence (a cluster of proline,
            glutamic acid, serine, and threonine residues), characteristic of
            proteins that undergo rapid intracellular degradation. The
            bromodomain is found in proteins that regulate transcription.
            Several transcript variants encoding multiple isoforms have been
            found for this gene. [provided by RefSeq, Jun 2011].
            
            Transcript Variant: This variant (1) differs in the 5' UTR and uses
            an alternate in-frame splice junction at the 3' end of an exon
            compared to variant 4. The resulting isoform (b) lacks a short
            internal segment compared to isoform a. Variants 1, 2, and 3 all
            encode the same isoform (b).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA1968968,
                              SAMEA2148093 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000399546.7/ ENSP00000387822.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-282               AC114486.2         74326-74607
            283-511             AC114486.2         87643-87871
            512-649             AC114486.2         89552-89689
            650-764             AC114486.2         93071-93185
            765-937             AC114486.2         101191-101363
            938-1288            AC114486.2         101968-102318
            1289-1417           AC114486.2         103093-103221
            1418-1606           AC114486.2         104494-104682
            1607-1779           AC114486.2         105568-105740
            1780-2069           AC114486.2         105814-106103
            2070-2183           AC114486.2         106193-106306
            2184-2321           AC114486.2         106543-106680
            2322-2383           AC114486.2         116109-116170
            2384-2434           AC114486.2         117189-117239
            2435-2606           AC114486.2         119008-119179
            2607-2707           AC114486.2         126974-127074
            2708-2913           AC114486.2         129339-129544
            2914-3094           AC114486.2         130044-130224
            3095-3310           AC114486.2         139131-139346
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p22.1"
     gene            1..3310
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /note="bromodomain testis associated"
                     /db_xref="GeneID:676"
                     /db_xref="HGNC:HGNC:1105"
                     /db_xref="MIM:602144"
     exon            1..282
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            283..511
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..3163
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /note="isoform b is encoded by transcript variant 1;
                     cancer/testis antigen 9; bromodomain testis-specific
                     protein; RING3-like protein; bromodomain, testis-specific"
                     /codon_start=1
                     /product="bromodomain testis-specific protein isoform b"
                     /protein_id="NP_997072.2"
                     /db_xref="CCDS:CCDS735.1"
                     /db_xref="GeneID:676"
                     /db_xref="HGNC:HGNC:1105"
                     /db_xref="MIM:602144"
                     /translation="MSLPSRQTAIIVNPPPPEYINTKKNGRLTNQLQYLQKVVLKDLW
                     KHSFSWPFQRPVDAVKLQLPDYYTIIKNPMDLNTIKKRLENKYYAKASECIEDFNTMF
                     SNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQVVGVKERIKKGTQQNIAVSSAK
                     EKSSPSATEKVFKQQEIPSVFPKTSISPLNVVQGASVNSSSQTAAQVTKGVKRKADTT
                     TPATSAVKASSEFSPTFTEKSVALPPIKENMPKNVLPDSQQQYNVVKTVKVTEQLRHC
                     SEILKEMLAKKHFSYAWPFYNPVDVNALGLHNYYDVVKNPMDLGTIKEKMDNQEYKDA
                     YKFAADVRLMFMNCYKYNPPDHEVVTMARMLQDVFETHFSKIPIEPVESMPLCYIKTD
                     ITETTGRENTNEASSEGNSSDDSEDERVKRLAKLQEQLKAVHQQLQVLSQVPFRKLNK
                     KKEKSKKEKKKEKVNNSNENPRKMCEQMRLKEKSKRNQPKKRKQQFIGLKSEDEDNAK
                     PMNYDEKRQLSLNINKLPGDKLGRVVHIIQSREPSLSNSNPDEIEIDFETLKASTLRE
                     LEKYVSACLRKRPLKPPAKKIMMSKEELHSQKKQELEKRLLDVNNQLNSRKRQTKSDK
                     TQPSKAVENVSRLSESSSSSSSSSESESSSSDLSSSDSSDSESEMFPKFTEVKPNDSP
                     SKENVKKMKNECIPPEGRTGVTQIGYCVQDTTSANTTLVHQTTPSHVMPPNHHQLAFN
                     YQELEHLQTVKNISPLQILPPSGDSEQLSNGITVMHPSGDSDTTMLESECQAPVQKDI
                     KIKNADSWKSLGKPVKPSGVMKSSDELFNQFRKAAIEKEVKARTQELIRKHLEQNTKE
                     LKASQENQRDLGNGLTVESFSNKIQNKCSGEEQKEHQQSSEAQDKSKLWLLKDRDLAR
                     QKEQERRRREAMVGTIDMTLQSDIMTMFENNFD"
     exon            512..649
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            650..764
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            765..937
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1288
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1417
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1606
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1779
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..2069
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2070..2183
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2184..2321
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2383
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2434
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2435..2606
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2607..2707
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2708..2913
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            2914..3094
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     exon            3095..3310
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3290..3295
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /note="hexamer: ATTAAA"
     polyA_site      3310
                     /gene="BRDT"
                     /gene_synonym="BRD6; CT9; SPGF21"
                     /note="major polyA site"
ORIGIN      
        1 acaacaaaaa ctggcggcga ggaactgcgg agaactgttg ccctgcaccg cttcattttg
       61 tgcagcctga aaggggcaat cacataaggt cggttttgta atatacaggt aacatacagg
      121 taatgtaccc tccacagggg gtgctgcctt aggccggccc cgaggtgcgg ccttgggtgg
      181 gaacaaccgt tttttccccc ctctcccctc cccgacccca tcaacatcgt gcgacagtcc
      241 gggcagttga gaaggccacc ccgcttcgtg cctgcagccc aggacatgta cttgtagaca
      301 ggattcaaag cagttaagaa tgtctctgcc aagtcgacaa acagctatta ttgttaaccc
      361 tcctccacca gaatatataa atactaagaa aaatgggcga ttgacaaatc aacttcagta
      421 tctacaaaaa gttgtcctaa aggatttatg gaagcatagt ttttcatggc cctttcaacg
      481 tcctgtggat gctgtgaaac tacagttgcc tgattattat accattataa aaaacccaat
      541 ggatttaaat acaattaaga agcgcttgga gaataaatat tatgcgaagg cttcagaatg
      601 tatagaagac ttcaatacaa tgttctcaaa ttgttattta tataacaagc ctggagatga
      661 cattgttctt atggcacaag ctctagagaa gctgtttatg cagaaattat ctcagatgcc
      721 acaagaagag caagttgtgg gtgttaagga aagaatcaag aaaggcactc aacagaatat
      781 agctgtttct tctgctaaag aaaaatcatc acccagcgca acagaaaaag tatttaagca
      841 gcaagaaatt ccttctgtat ttcctaagac atctatttct cccttgaacg tggtacaggg
      901 agcttcagtc aactccagtt cacaaactgc ggcccaagtt acaaaaggtg tgaagaggaa
      961 agcagataca acaactcctg caacttcagc agttaaagca agtagtgaat tttctccaac
     1021 attcacagaa aaatcagtgg cactgccacc tataaaagaa aatatgccaa agaatgtttt
     1081 gccagattct cagcaacaat ataatgttgt gaagactgtt aaagtaactg aacaattaag
     1141 gcactgtagt gagattctta aagaaatgct tgcaaagaaa catttttcat atgcatggcc
     1201 cttttataat cctgttgacg ttaatgcttt gggactccat aactactatg acgttgtcaa
     1261 aaatccgatg gatcttggaa ctattaagga gaaaatggat aaccaagaat ataaggatgc
     1321 atacaaattt gcggcagatg ttagattaat gttcatgaat tgctacaagt acaatcctcc
     1381 agatcacgaa gttgtgacaa tggcaagaat gcttcaggat gttttcgaaa cgcatttttc
     1441 aaagatcccg attgaacctg ttgagagtat gcctttatgt tacatcaaaa cagatatcac
     1501 agaaaccact ggtagagaga acactaatga agcctcctct gaagggaact cttctgatga
     1561 ttctgaagat gagcgagtta agcgtcttgc aaagcttcag gagcagctta aagctgtaca
     1621 tcaacagctc caggttttgt cccaagtacc tttccgtaag ctaaataaaa agaaagagaa
     1681 gtctaaaaag gaaaagaaaa aagaaaaggt taataacagc aatgaaaatc caagaaaaat
     1741 gtgtgagcaa atgaggctaa aggaaaagtc caagagaaat cagccaaaga aaaggaaaca
     1801 acagttcatt ggtctaaaat ctgaagatga agataatgct aaacctatga actatgatga
     1861 gaaaaggcag ttaagtctga atataaacaa actccctgga gataaacttg ggcgagtagt
     1921 tcacataata caatcaagag agccttctct gagcaattcc aatcctgatg agatagagat
     1981 agactttgaa acactgaaag catcaacact aagagaatta gaaaaatatg tttcggcatg
     2041 tctaagaaag agaccattaa aacctcctgc taagaaaata atgatgtcca aagaagaact
     2101 tcactcacag aaaaaacagg aattggaaaa gcggttactg gatgttaata atcagttaaa
     2161 ttctagaaaa cgtcaaacaa aatctgataa aacgcaacca tccaaagctg ttgaaaatgt
     2221 ttcccgactg agtgagagca gcagcagcag cagcagctca tcagagtctg aaagtagcag
     2281 cagtgactta agctcttcag acagcagtga ttctgaatca gaaatgttcc ctaagtttac
     2341 agaagtaaaa ccaaatgatt ctccttctaa agagaatgta aagaaaatga agaatgaatg
     2401 cataccgcct gaaggaagaa caggcgtcac acagatagga tattgtgtgc aagacacaac
     2461 ctctgccaat actacccttg ttcatcagac cacaccttca catgtaatgc caccaaatca
     2521 ccaccaatta gcatttaatt atcaagaatt agaacattta cagactgtga aaaacatttc
     2581 acctttacaa attctgcctc cctcaggtga ttctgaacag ctctcaaatg gcataactgt
     2641 gatgcatcca tctggtgata gtgacacaac gatgttagaa tctgaatgtc aagctcctgt
     2701 acagaaggat ataaagatta agaatgcaga ttcatggaaa agtttaggca aaccagtgaa
     2761 accatcaggt gtaatgaaat cctcagatga gctcttcaac caatttagaa aagcagccat
     2821 agaaaaggaa gtaaaagctc ggacacagga actcatacgg aagcatttgg aacaaaatac
     2881 aaaggaacta aaagcatctc aagaaaatca gagggatctt gggaatggat tgactgtaga
     2941 atctttttca aataaaatac aaaacaagtg ctctggagaa gagcagaaag aacatcagca
     3001 gtcatcagaa gctcaagata aatccaaact ctggcttctc aaagaccgtg atttagcaag
     3061 gcagaaagaa caagagagga ggaggagaga agcaatggtg ggtaccattg atatgaccct
     3121 tcaaagtgac attatgacaa tgtttgaaaa caactttgat taaaactcag tttttaaatt
     3181 aaccatcaac ttaaaatgaa tggtaaaaga tcaaaatgca tatggtaaaa tgattgcttt
     3241 cagataacaa gataccaatc ttatattgta ttttgactgc tctaaaatga ttaaacagtt
     3301 ttcacttaca
//
