LOCUS       NM_001407739            7204 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens BRCA1 DNA repair associated (BRCA1), transcript
            variant 122, mRNA.
ACCESSION   NM_001407739
VERSION     NM_001407739.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Orban,T.I. and Olah,E.
  TITLE     Emerging roles of BRCA1 alternative splicing
  JOURNAL   Mol Pathol 56 (4), 191-197 (2003)
   PUBMED   12890739
  REMARK    Review article
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Orban,T.I. and Olah,E.
  TITLE     Expression profiles of BRCA1 splice variants in asynchronous and in
            G1/S synchronized tumor cell lines
  JOURNAL   Biochem Biophys Res Commun 280 (1), 32-38 (2001)
   PUBMED   11162473
REFERENCE   3  (bases 1 to 7204)
  AUTHORS   Paterson,J.W.
  TITLE     BRCA1: a review of structure and putative functions
  JOURNAL   Dis Markers 13 (4), 261-274 (1998)
   PUBMED   9553742
  REMARK    Review article
REFERENCE   4  (bases 1 to 7204)
  AUTHORS   Xu,C.F., Chambers,J.A., Nicolai,H., Brown,M.A., Hujeirat,Y.,
            Mohammed,S., Hodgson,S., Kelsell,D.P., Spurr,N.K., Bishop,D.T. and
            Solomon,E.
  TITLE     Mutations and alternative splicing of the BRCA1 gene in UK
            breast/ovarian cancer families
  JOURNAL   Genes Chromosomes Cancer 18 (2), 102-110 (1997)
   PUBMED   9115959
REFERENCE   5  (bases 1 to 7204)
  AUTHORS   Wilson,C.A., Payton,M.N., Elliott,G.S., Buaas,F.W., Cajulis,E.E.,
            Grosshans,D., Ramos,L., Reese,D.M., Slamon,D.J. and Calzone,F.J.
  TITLE     Differential subcellular localization, expression and biological
            toxicity of BRCA1 and the splice variant BRCA1-delta11b
  JOURNAL   Oncogene 14 (1), 1-16 (1997)
   PUBMED   9010228
REFERENCE   6  (bases 1 to 7204)
  AUTHORS   Thakur,S., Zhang,H.B., Peng,Y., Le,H., Carroll,B., Ward,T., Yao,J.,
            Farid,L.M., Couch,F.J., Wilson,R.B. and Weber,B.L.
  TITLE     Localization of BRCA1 and a splice variant identifies the nuclear
            localization signal
  JOURNAL   Mol Cell Biol 17 (1), 444-452 (1997)
   PUBMED   8972225
REFERENCE   7  (bases 1 to 7204)
  AUTHORS   Lu,M., Conzen,S.D., Cole,C.N. and Arrick,B.A.
  TITLE     Characterization of functional messenger RNA splice variants of
            BRCA1 expressed in nonmalignant and tumor-derived breast cells
  JOURNAL   Cancer Res 56 (20), 4578-4581 (1996)
   PUBMED   8840964
REFERENCE   8  (bases 1 to 7204)
  AUTHORS   Holt,J.T., Thompson,M.E., Szabo,C., Robinson-Benion,C.,
            Arteaga,C.L., King,M.C. and Jensen,R.A.
  TITLE     Growth retardation and tumour inhibition by BRCA1
  JOURNAL   Nat Genet 12 (3), 298-302 (1996)
   PUBMED   8589721
  REMARK    Erratum:[Nat Genet 1998 May;19(1):102]
REFERENCE   9  (bases 1 to 7204)
  AUTHORS   Xu,C.F., Brown,M.A., Chambers,J.A., Griffiths,B., Nicolai,H. and
            Solomon,E.
  TITLE     Distinct transcription start sites generate two forms of BRCA1 mRNA
  JOURNAL   Hum Mol Genet 4 (12), 2259-2264 (1995)
   PUBMED   8634696
REFERENCE   10 (bases 1 to 7204)
  AUTHORS   Miki,Y., Swensen,J., Shattuck-Eidens,D., Futreal,P.A., Harshman,K.,
            Tavtigian,S., Liu,Q., Cochran,C., Bennett,L.M., Ding,W. et al.
  TITLE     A strong candidate for the breast and ovarian cancer susceptibility
            gene BRCA1
  JOURNAL   Science 266 (5182), 66-71 (1994)
   PUBMED   7545954
REFERENCE   11 (bases 1 to 7204)
  AUTHORS   Mehta,P.A. and Ebens,C.
  TITLE     Fanconi Anemia
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301575
REFERENCE   12 (bases 1 to 7204)
  AUTHORS   Petrucelli,N., Daly,M.B. and Pal,T.
  TITLE     BRCA1- and BRCA2-Associated Hereditary Breast and Ovarian Cancer
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301425
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC135721.4.
            
            Summary: This gene encodes a 190 kD nuclear phosphoprotein that
            plays a role in maintaining genomic stability, and it also acts as
            a tumor suppressor. The BRCA1 gene contains 22 exons spanning about
            110 kb of DNA. The encoded protein combines with other tumor
            suppressors, DNA damage sensors, and signal transducers to form a
            large multi-subunit protein complex known as the BRCA1-associated
            genome surveillance complex (BASC). This gene product associates
            with RNA polymerase II, and through the C-terminal domain, also
            interacts with histone deacetylase complexes. This protein thus
            plays a role in transcription, DNA repair of double-stranded
            breaks, and recombination. Mutations in this gene are responsible
            for approximately 40% of inherited breast cancers and more than 80%
            of inherited breast and ovarian cancers. Alternative splicing plays
            a role in modulating the subcellular localization and physiological
            function of this gene. Many alternatively spliced transcript
            variants, some of which are disease-associated mutations, have been
            described for this gene, but the full-length natures of only some
            of these variants has been described. A related pseudogene, which
            is also located on chromosome 17, has been identified. [provided by
            RefSeq, May 2020].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2507095.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                AC135721.4         34056-34149
            95-193              AC135721.4         35305-35403
            194-312             AC135721.4         37744-37862
            313-366             AC135721.4         43641-43694
            367-444             AC135721.4         52887-52964
            445-533             AC135721.4         54464-54552
            534-673             AC135721.4         55159-55298
            674-779             AC135721.4         59540-59645
            780-825             AC135721.4         62131-62176
            826-902             AC135721.4         63498-63574
            903-4328            AC135721.4         64560-67985
            4329-4417           AC135721.4         68388-68476
            4418-4589           AC135721.4         76845-77016
            4590-4713           AC135721.4         82809-82932
            4714-4904           AC135721.4         84899-85089
            4905-5215           AC135721.4         88182-88492
            5216-5303           AC135721.4         91725-91812
            5304-5381           AC135721.4         95469-95546
            5382-5422           AC135721.4         96047-96087
            5423-5506           AC135721.4         102285-102368
            5507-5561           AC135721.4         108303-108357
            5562-5635           AC135721.4         110226-110299
            5636-5696           AC135721.4         111717-111777
            5697-7204           AC135721.4         113618-115125
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.31"
     gene            1..7204
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /note="BRCA1 DNA repair associated"
                     /db_xref="GeneID:672"
                     /db_xref="HGNC:HGNC:1100"
                     /db_xref="MIM:113705"
     exon            1..94
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            95..193
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            194..312
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            313..366
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            367..444
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     CDS             374..5821
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /EC_number="2.3.2.27"
                     /note="isoform 36 is encoded by transcript variant 122;
                     BRCA1/BRCA2-containing complex, subunit 1; RING finger
                     protein 53; breast cancer type 1 susceptibility protein;
                     breast and ovarian cancer susceptibility protein 1;
                     protein phosphatase 1, regulatory subunit 53; Fanconi
                     anemia, complementation group S; early onset breast cancer
                     1; breast cancer 1, early onset"
                     /codon_start=1
                     /product="breast cancer type 1 susceptibility protein
                     isoform 36"
                     /protein_id="NP_001394668.1"
                     /db_xref="GeneID:672"
                     /db_xref="HGNC:HGNC:1100"
                     /db_xref="MIM:113705"
                     /translation="MLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIC
                     AFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQE
                     TSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQI
                     TPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSS
                     VSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAG
                     SKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQK
                     VNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEA
                     LICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERP
                     LTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHEN
                     KTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRR
                     KSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEG
                     KEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPRE
                     EKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLE
                     VSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSI
                     EMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFEC
                     EQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGN
                     ETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENI
                     PSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRG
                     PKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQ
                     PMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPF
                     THTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATEC
                     LSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTAN
                     TNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGE
                     AASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQP
                     SNSYPSIISDSSALEDLRNPEQSTSEKVLTSQKSSEYPISQNPEGLSADKFEVSADSS
                     TSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEES
                     GPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSAL
                     KVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLT
                     PEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSY
                     FWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTN
                     MPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPV
                     VTREWVLDSVALYQCQELDTYLIPQIPHSHY"
     exon            445..533
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            534..673
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            674..779
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            780..825
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            826..902
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            903..4328
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            4329..4417
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            4418..4589
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            4590..4713
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            4714..4904
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            4905..5215
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5216..5303
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5304..5381
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5382..5422
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5423..5506
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5507..5561
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5562..5635
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5636..5696
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     exon            5697..7204
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7179..7184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /note="hexamer: AATAAA"
     polyA_site      7204
                     /gene="BRCA1"
                     /gene_synonym="BRCAI; BRCC1; BROVCA1; FANCS; IRIS; PNCA4;
                     PPP1R53; PSCP; RNF53"
                     /note="major polyA site"
ORIGIN      
        1 gctgagactt cctggacggg ggacaggctg tggggtttct cagataactg ggcccctgcg
       61 ctcaggaggc cttcaccctc tgctctgggt aaagttcatt ggaacagaaa gaaatggatt
      121 tatctgctct tcgcgttgaa gaagtacaaa atgtcattaa tgctatgcag aaaatcttag
      181 agtgtcccat ctgactttgg ctcttgttgc ccaggctgga gtgcagtggc acaatctcgg
      241 ctcactacaa cctccacctc ccgggttcaa gcaattctgc tgcctcagcc tccggagtac
      301 ctgggattgc agtctggagt tgatcaagga acctgtctcc acaaagtgtg accacatatt
      361 ttgcaaattt tgcatgctga aacttctcaa ccagaagaaa gggccttcac agtgtccttt
      421 atgtaagaat gatataacca aaaggagcct acaagaaagt acgagattta gtcaacttgt
      481 tgaagagcta ttgaaaatca tttgtgcttt tcagcttgac acaggtttgg agtatgcaaa
      541 cagctataat tttgcaaaaa aggaaaataa ctctcctgaa catctaaaag atgaagtttc
      601 tatcatccaa agtatgggct acagaaaccg tgccaaaaga cttctacaga gtgaacccga
      661 aaatccttcc ttgcaggaaa ccagtctcag tgtccaactc tctaaccttg gaactgtgag
      721 aactctgagg acaaagcagc ggatacaacc tcaaaagacg tctgtctaca ttgaattggg
      781 atctgattct tctgaagata ccgttaataa ggcaacttat tgcagtgtgg gagatcaaga
      841 attgttacaa atcacccctc aaggaaccag ggatgaaatc agtttggatt ctgcaaaaaa
      901 ggctgcttgt gaattttctg agacggatgt aacaaatact gaacatcatc aacccagtaa
      961 taatgatttg aacaccactg agaagcgtgc agctgagagg catccagaaa agtatcaggg
     1021 tagttctgtt tcaaacttgc atgtggagcc atgtggcaca aatactcatg ccagctcatt
     1081 acagcatgag aacagcagtt tattactcac taaagacaga atgaatgtag aaaaggctga
     1141 attctgtaat aaaagcaaac agcctggctt agcaaggagc caacataaca gatgggctgg
     1201 aagtaaggaa acatgtaatg ataggcggac tcccagcaca gaaaaaaagg tagatctgaa
     1261 tgctgatccc ctgtgtgaga gaaaagaatg gaataagcag aaactgccat gctcagagaa
     1321 tcctagagat actgaagatg ttccttggat aacactaaat agcagcattc agaaagttaa
     1381 tgagtggttt tccagaagtg atgaactgtt aggttctgat gactcacatg atggggagtc
     1441 tgaatcaaat gccaaagtag ctgatgtatt ggacgttcta aatgaggtag atgaatattc
     1501 tggttcttca gagaaaatag acttactggc cagtgatcct catgaggctt taatatgtaa
     1561 aagtgaaaga gttcactcca aatcagtaga gagtaatatt gaagacaaaa tatttgggaa
     1621 aacctatcgg aagaaggcaa gcctccccaa cttaagccat gtaactgaaa atctaattat
     1681 aggagcattt gttactgagc cacagataat acaagagcgt cccctcacaa ataaattaaa
     1741 gcgtaaaagg agacctacat caggccttca tcctgaggat tttatcaaga aagcagattt
     1801 ggcagttcaa aagactcctg aaatgataaa tcagggaact aaccaaacgg agcagaatgg
     1861 tcaagtgatg aatattacta atagtggtca tgagaataaa acaaaaggtg attctattca
     1921 gaatgagaaa aatcctaacc caatagaatc actcgaaaaa gaatctgctt tcaaaacgaa
     1981 agctgaacct ataagcagca gtataagcaa tatggaactc gaattaaata tccacaattc
     2041 aaaagcacct aaaaagaata ggctgaggag gaagtcttct accaggcata ttcatgcgct
     2101 tgaactagta gtcagtagaa atctaagccc acctaattgt actgaattgc aaattgatag
     2161 ttgttctagc agtgaagaga taaagaaaaa aaagtacaac caaatgccag tcaggcacag
     2221 cagaaaccta caactcatgg aaggtaaaga acctgcaact ggagccaaga agagtaacaa
     2281 gccaaatgaa cagacaagta aaagacatga cagcgatact ttcccagagc tgaagttaac
     2341 aaatgcacct ggttctttta ctaagtgttc aaataccagt gaacttaaag aatttgtcaa
     2401 tcctagcctt ccaagagaag aaaaagaaga gaaactagaa acagttaaag tgtctaataa
     2461 tgctgaagac cccaaagatc tcatgttaag tggagaaagg gttttgcaaa ctgaaagatc
     2521 tgtagagagt agcagtattt cattggtacc tggtactgat tatggcactc aggaaagtat
     2581 ctcgttactg gaagttagca ctctagggaa ggcaaaaaca gaaccaaata aatgtgtgag
     2641 tcagtgtgca gcatttgaaa accccaaggg actaattcat ggttgttcca aagataatag
     2701 aaatgacaca gaaggcttta agtatccatt gggacatgaa gttaaccaca gtcgggaaac
     2761 aagcatagaa atggaagaaa gtgaacttga tgctcagtat ttgcagaata cattcaaggt
     2821 ttcaaagcgc cagtcatttg ctccgttttc aaatccagga aatgcagaag aggaatgtgc
     2881 aacattctct gcccactctg ggtccttaaa gaaacaaagt ccaaaagtca cttttgaatg
     2941 tgaacaaaag gaagaaaatc aaggaaagaa tgagtctaat atcaagcctg tacagacagt
     3001 taatatcact gcaggctttc ctgtggttgg tcagaaagat aagccagttg ataatgccaa
     3061 atgtagtatc aaaggaggct ctaggttttg tctatcatct cagttcagag gcaacgaaac
     3121 tggactcatt actccaaata aacatggact tttacaaaac ccatatcgta taccaccact
     3181 ttttcccatc aagtcatttg ttaaaactaa atgtaagaaa aatctgctag aggaaaactt
     3241 tgaggaacat tcaatgtcac ctgaaagaga aatgggaaat gagaacattc caagtacagt
     3301 gagcacaatt agccgtaata acattagaga aaatgttttt aaagaagcca gctcaagcaa
     3361 tattaatgaa gtaggttcca gtactaatga agtgggctcc agtattaatg aaataggttc
     3421 cagtgatgaa aacattcaag cagaactagg tagaaacaga gggccaaaat tgaatgctat
     3481 gcttagatta ggggttttgc aacctgaggt ctataaacaa agtcttcctg gaagtaattg
     3541 taagcatcct gaaataaaaa agcaagaata tgaagaagta gttcagactg ttaatacaga
     3601 tttctctcca tatctgattt cagataactt agaacagcct atgggaagta gtcatgcatc
     3661 tcaggtttgt tctgagacac ctgatgacct gttagatgat ggtgaaataa aggaagatac
     3721 tagttttgct gaaaatgaca ttaaggaaag ttctgctgtt tttagcaaaa gcgtccagaa
     3781 aggagagctt agcaggagtc ctagcccttt cacccataca catttggctc agggttaccg
     3841 aagaggggcc aagaaattag agtcctcaga agagaactta tctagtgagg atgaagagct
     3901 tccctgcttc caacacttgt tatttggtaa agtaaacaat ataccttctc agtctactag
     3961 gcatagcacc gttgctaccg agtgtctgtc taagaacaca gaggagaatt tattatcatt
     4021 gaagaatagc ttaaatgact gcagtaacca ggtaatattg gcaaaggcat ctcaggaaca
     4081 tcaccttagt gaggaaacaa aatgttctgc tagcttgttt tcttcacagt gcagtgaatt
     4141 ggaagacttg actgcaaata caaacaccca ggatcctttc ttgattggtt cttccaaaca
     4201 aatgaggcat cagtctgaaa gccagggagt tggtctgagt gacaaggaat tggtttcaga
     4261 tgatgaagaa agaggaacgg gcttggaaga aaataatcaa gaagagcaaa gcatggattc
     4321 aaacttaggt gaagcagcat ctgggtgtga gagtgaaaca agcgtctctg aagactgctc
     4381 agggctatcc tctcagagtg acattttaac cactcagcag agggatacca tgcaacataa
     4441 cctgataaag ctccagcagg aaatggctga actagaagct gtgttagaac agcatgggag
     4501 ccagccttct aacagctacc cttccatcat aagtgactct tctgcccttg aggacctgcg
     4561 aaatccagaa caaagcacat cagaaaaagt attaacttca cagaaaagta gtgaataccc
     4621 tataagccag aatccagaag gcctttctgc tgacaagttt gaggtgtctg cagatagttc
     4681 taccagtaaa aataaagaac caggagtgga aaggtcatcc ccttctaaat gcccatcatt
     4741 agatgatagg tggtacatgc acagttgctc tgggagtctt cagaatagaa actacccatc
     4801 tcaagaggag ctcattaagg ttgttgatgt ggaggagcaa cagctggaag agtctgggcc
     4861 acacgatttg acggaaacat cttacttgcc aaggcaagat ctagagggaa ccccttacct
     4921 ggaatctgga atcagcctct tctctgatga ccctgaatct gatccttctg aagacagagc
     4981 cccagagtca gctcgtgttg gcaacatacc atcttcaacc tctgcattga aagttcccca
     5041 attgaaagtt gcagaatctg cccagagtcc agctgctgct catactactg atactgctgg
     5101 gtataatgca atggaagaaa gtgtgagcag ggagaagcca gaattgacag cttcaacaga
     5161 aagggtcaac aaaagaatgt ccatggtggt gtctggcctg accccagaag aatttatgct
     5221 cgtgtacaag tttgccagaa aacaccacat cactttaact aatctaatta ctgaagagac
     5281 tactcatgtt gttatgaaaa cagatgctga gtttgtgtgt gaacggacac tgaaatattt
     5341 tctaggaatt gcgggaggaa aatgggtagt tagctatttc tgggtgaccc agtctattaa
     5401 agaaagaaaa atgctgaatg agcatgattt tgaagtcaga ggagatgtgg tcaatggaag
     5461 aaaccaccaa ggtccaaagc gagcaagaga atcccaggac agaaagatct tcagggggct
     5521 agaaatctgt tgctatgggc ccttcaccaa catgcccaca gatcaactgg aatggatggt
     5581 acagctgtgt ggtgcttctg tggtgaagga gctttcatca ttcacccttg gcacaggtgt
     5641 ccacccaatt gtggttgtgc agccagatgc ctggacagag gacaatggct tccatgcaat
     5701 tgggcagatg tgtgaggcac ctgtggtgac ccgagagtgg gtgttggaca gtgtagcact
     5761 ctaccagtgc caggagctgg acacctacct gataccccag atcccccaca gccactactg
     5821 actgcagcca gccacaggta cagagccaca ggaccccaag aatgagctta caaagtggcc
     5881 tttccaggcc ctgggagctc ctctcactct tcagtccttc tactgtcctg gctactaaat
     5941 attttatgta catcagcctg aaaaggactt ctggctatgc aagggtccct taaagatttt
     6001 ctgcttgaag tctcccttgg aaatctgcca tgagcacaaa attatggtaa tttttcacct
     6061 gagaagattt taaaaccatt taaacgccac caattgagca agatgctgat tcattattta
     6121 tcagccctat tctttctatt caggctgttg ttggcttagg gctggaagca cagagtggct
     6181 tggcctcaag agaatagctg gtttccctaa gtttacttct ctaaaaccct gtgttcacaa
     6241 aggcagagag tcagaccctt caatggaagg agagtgcttg ggatcgatta tgtgacttaa
     6301 agtcagaata gtccttgggc agttctcaaa tgttggagtg gaacattggg gaggaaattc
     6361 tgaggcaggt attagaaatg aaaaggaaac ttgaaacctg ggcatggtgg ctcacgcctg
     6421 taatcccagc actttgggag gccaaggtgg gcagatcact ggaggtcagg agttcgaaac
     6481 cagcctggcc aacatggtga aaccccatct ctactaaaaa tacagaaatt agccggtcat
     6541 ggtggtggac acctgtaatc ccagctactc aggtggctaa ggcaggagaa tcacttcagc
     6601 ccgggaggtg gaggttgcag tgagccaaga tcataccacg gcactccagc ctgggtgaca
     6661 gtgagactgt ggctcaaaaa aaaaaaaaaa aaaaggaaaa tgaaactaga agagatttct
     6721 aaaagtctga gatatatttg ctagatttct aaagaatgtg ttctaaaaca gcagaagatt
     6781 ttcaagaacc ggtttccaaa gacagtcttc taattcctca ttagtaataa gtaaaatgtt
     6841 tattgttgta gctctggtat ataatccatt cctcttaaaa tataagacct ctggcatgaa
     6901 tatttcatat ctataaaatg acagatccca ccaggaagga agctgttgct ttctttgagg
     6961 tgattttttt cctttgctcc ctgttgctga aaccatacag cttcataaat aattttgctt
     7021 gctgaaggaa gaaaaagtgt ttttcataaa cccattatcc aggactgttt atagctgttg
     7081 gaaggactag gtcttcccta gcccccccag tgtgcaaggg cagtgaagac ttgattgtac
     7141 aaaatacgtt ttgtaaatgt tgtgctgtta acactgcaaa taaacttggt agcaaacact
     7201 tcca
//
