LOCUS       NM_004333               6459 bp    mRNA    linear   PRI 05-DEC-2024
DEFINITION  Homo sapiens B-Raf proto-oncogene, serine/threonine kinase (BRAF),
            transcript variant 1, mRNA.
ACCESSION   NM_004333
VERSION     NM_004333.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Valcikova,B., Vadovicova,N., Smolkova,K., Zacpalova,M., Krejci,P.,
            Lee,S., Rauch,J., Kolch,W., von Kriegsheim,A., Dorotikova,A.,
            Andrysik,Z., Vichova,R., Vacek,O., Soucek,K. and Uldrijan,S.
  TITLE     eIF4F controls ERK MAPK signaling in melanomas with BRAF and NRAS
            mutations
  JOURNAL   Proc Natl Acad Sci U S A 121 (44), e2321305121 (2024)
   PUBMED   39436655
  REMARK    GeneRIF: eIF4F controls ERK MAPK signaling in melanomas with BRAF
            and NRAS mutations.
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   von Ammon,J.L., Machado,G.J.R., da Matta,R.R.C., Telles,A.C.,
            Carrijo,F., Dos Santos,B.A.F., Brandao,J.C.D., da Silva,T.M.,
            Hecht,F., Colozza-Gama,G.A., Tezzei,J.H., Cerutti,J.M. and
            Ramos,H.E.
  TITLE     Follicular cell-derived thyroid carcinomas harboring novel genetic
            BRAFNON-V600E mutations: real-world data obtained using a multigene
            panel
  JOURNAL   Arch Endocrinol Metab 68, e240067 (2024)
   PUBMED   39420942
  REMARK    GeneRIF: Follicular cell-derived thyroid carcinomas harboring novel
            genetic BRAF[NON-V600E] mutations: real-world data obtained using a
            multigene panel.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6459)
  AUTHORS   Langabeer,S.E.
  TITLE     Non-canonical BRAF variants and rearrangements in hairy cell
            leukemia
  JOURNAL   Oncol Res 32 (9), 1423-1427 (2024)
   PUBMED   39220131
  REMARK    GeneRIF: Non-canonical BRAF variants and rearrangements in hairy
            cell leukemia.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6459)
  AUTHORS   Gripp,K.W. and Weaver,K.N.
  TITLE     HRAS-Related Costello Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301680
REFERENCE   5  (bases 1 to 6459)
  AUTHORS   Gelb,B.D. and Tartaglia,M.
  TITLE     Noonan Syndrome with Multiple Lentigines
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301557
REFERENCE   6  (bases 1 to 6459)
  AUTHORS   Rauen,K.A.
  TITLE     Cardiofaciocutaneous Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301365
REFERENCE   7  (bases 1 to 6459)
  AUTHORS   Roberts,A.E.
  TITLE     Noonan Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301303
REFERENCE   8  (bases 1 to 6459)
  AUTHORS   Stephens,R.M., Sithanandam,G., Copeland,T.D., Kaplan,D.R.,
            Rapp,U.R. and Morrison,D.K.
  TITLE     95-kilodalton B-Raf serine/threonine kinase: identification of the
            protein and its major autophosphorylation site
  JOURNAL   Mol Cell Biol 12 (9), 3733-3742 (1992)
   PUBMED   1508179
REFERENCE   9  (bases 1 to 6459)
  AUTHORS   Eychene,A., Barnier,J.V., Apiou,F., Dutrillaux,B. and Calothy,G.
  TITLE     Chromosomal assignment of two human B-raf(Rmil) proto-oncogene
            loci: B-raf-1 encoding the p94Braf/Rmil and B-raf-2, a processed
            pseudogene
  JOURNAL   Oncogene 7 (8), 1657-1660 (1992)
   PUBMED   1630826
REFERENCE   10 (bases 1 to 6459)
  AUTHORS   Sithanandam,G., Druck,T., Cannizzaro,L.A., Leuzzi,G., Huebner,K.
            and Rapp,U.R.
  TITLE     B-raf and a B-raf pseudogene are located on 7q in man
  JOURNAL   Oncogene 7 (4), 795-799 (1992)
   PUBMED   1565476
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006006.2, DB454480.1,
            BC101757.1, M95712.2, AW971583.1 and AC006344.2.
            
            On Jun 2, 2019 this sequence version replaced NM_004333.5.
            
            Summary: This gene encodes a protein belonging to the RAF family of
            serine/threonine protein kinases. This protein plays a role in
            regulating the MAP kinase/ERK signaling pathway, which affects cell
            division, differentiation, and secretion. Mutations in this gene,
            most commonly the V600E mutation, are the most frequently
            identified cancer-causing mutations in melanoma, and have been
            identified in various other cancers as well, including non-Hodgkin
            lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung
            carcinoma, hairy cell leukemia and adenocarcinoma of lung.
            Mutations in this gene are also associated with
            cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit
            overlapping phenotypes. A pseudogene of this gene has been
            identified on the X chromosome. [provided by RefSeq, Aug 2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M95712.2, BC101757.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000646891.2/ ENSP00000493543.1
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AC006006.2         44051-44051         c
            2-204               DB454480.1         1-203
            205-2570            BC101757.1         1-2366
            2571-2635           M95712.2           2406-2470
            2636-2970           AW971583.1         139-473             c
            2971-6459           AC006344.2         47430-50918         c
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q34"
     gene            1..6459
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="B-Raf proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:673"
                     /db_xref="HGNC:HGNC:1097"
                     /db_xref="MIM:164757"
     exon            1..364
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             227..2527
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase B-raf; B-Raf
                     serine/threonine-protein; B-Raf proto-oncogene
                     serine/threonine-protein kinase (p94); murine sarcoma
                     viral (v-raf) oncogene homolog B1; v-raf murine sarcoma
                     viral oncogene homolog B1; 94 kDa B-raf protein;
                     proto-oncogene B-Raf"
                     /codon_start=1
                     /product="serine/threonine-protein kinase B-raf isoform 1"
                     /protein_id="NP_004324.2"
                     /db_xref="CCDS:CCDS5863.1"
                     /db_xref="GeneID:673"
                     /db_xref="HGNC:HGNC:1097"
                     /db_xref="MIM:164757"
                     /translation="MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIP
                     EEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLES
                     LGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVF
                     LPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLT
                     GEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEV
                     PLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSP
                     SPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDG
                     GSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDW
                     EIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKT
                     RHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDY
                     LHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEV
                     IRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVR
                     SNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQT
                     EDFSLYACASPKTPIQAGGYGAFPVH"
     exon            365..466
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            467..730
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            731..834
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            835..937
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1086
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1206
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1366
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1403
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1540
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1658
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1743
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1920
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..1967
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2086
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            2087..2218
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            2219..2353
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     exon            2354..6459
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2575..2580
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATGAA"
     polyA_site      2603
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="major polyA site"
     regulatory      2607..2612
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATAAA"
     polyA_site      2642
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="major polyA site"
     regulatory      3088..3093
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATAAA"
     polyA_site      3112
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="major polyA site"
     regulatory      3241..3246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATACA"
     polyA_site      3264
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
     regulatory      4767..4772
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATAAA"
     polyA_site      4794
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
     regulatory      6330..6335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATAAA"
     polyA_site      6355
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
     regulatory      6437..6442
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
                     /note="hexamer: AATAAA"
     polyA_site      6459
                     /gene="BRAF"
                     /gene_synonym="B-raf; B-RAF1; BRAF-1; BRAF1; NS7; RAFB1"
ORIGIN      
        1 cttcccccaa tcccctcagg ctcggctgcg cccggggccg cgggccggta cctgaggtgg
       61 cccaggcgcc ctccgcccgc ggcgccgccc gggccgctcc tccccgcgcc ccccgcgccc
      121 cccgctcctc cgcctccgcc tccgcctccg cctcccccag ctctccgcct cccttccccc
      181 tccccgcccg acagcggccg ctcgggcccc ggctctcggt tataagatgg cggcgctgag
      241 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc
      301 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga
      361 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt
      421 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata
      481 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa
      541 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc
      601 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc
      661 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca
      721 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact
      781 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa
      841 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga
      901 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt
      961 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg
     1021 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca
     1081 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc
     1141 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc
     1201 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc
     1261 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc
     1321 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga
     1381 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc
     1441 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga
     1501 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga
     1561 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattggatc
     1621 tggatcattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt
     1681 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact
     1741 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact
     1801 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga
     1861 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga
     1921 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca
     1981 tgaagacctc acagtaaaaa taggtgattt tggtctagct acagtgaaat ctcgatggag
     2041 tgggtcccat cagtttgaac agttgtctgg atccattttg tggatggcac cagaagtcat
     2101 cagaatgcaa gataaaaatc catacagctt tcagtcagat gtatatgcat ttggaattgt
     2161 tctgtatgaa ttgatgactg gacagttacc ttattcaaac atcaacaaca gggaccagat
     2221 aatttttatg gtgggacgag gatacctgtc tccagatctc agtaaggtac ggagtaactg
     2281 tccaaaagcc atgaagagat taatggcaga gtgcctcaaa aagaaaagag atgagagacc
     2341 actctttccc caaattctcg cctctattga gctgctggcc cgctcattgc caaaaattca
     2401 ccgcagtgca tcagaaccct ccttgaatcg ggctggtttc caaacagagg attttagtct
     2461 atatgcttgt gcttctccaa aaacacccat ccaggcaggg ggatatggtg cgtttcctgt
     2521 ccactgaaac aaatgagtga gagagttcag gagagtagca acaaaaggaa aataaatgaa
     2581 catatgtttg cttatatgtt aaattgaata aaatactctc ttttttttta aggtgaacca
     2641 aagaacactt gtgtggttaa agactagata taatttttcc ccaaactaaa atttatactt
     2701 aacattggat ttttaacatc caagggttaa aatacataga cattgctaaa aattggcaga
     2761 gcctcttcta gaggctttac tttctgttcc gggtttgtat cattcacttg gttattttaa
     2821 gtagtaaact tcagtttctc atgcaacttt tgttgccagc tatcacatgt ccactaggga
     2881 ctccagaaga agaccctacc tatgcctgtg tttgcaggtg agaagttggc agtcggttag
     2941 cctgggttag ataaggcaaa ctgaacagat ctaatttagg aagtcagtag aatttaataa
     3001 ttctattatt attcttaata atttttctat aactatttct ttttataaca atttggaaaa
     3061 tgtggatgtc ttttatttcc ttgaagcaat aaactaagtt tctttttata aattttgagt
     3121 gcaggtgacc aaaaatattg ctgaggagtg gcacgtttga catgagtaaa atgtcttaac
     3181 ttcggatttt tagcgggaaa atgttataaa ttggagtttc ttttaaatag ctttttttaa
     3241 aatacattaa ggatgtctcg ctcatgtaga agtcaaattt tgttgcaaac gcattgctcc
     3301 cttcacaccc aatctctccc ctgcaaaaaa tcttcacaga attctgtgag aacttttagg
     3361 tgtgtttttc tttgagatac ctctggttgc caaacaccag gtaatagatt ttttaaagtt
     3421 gttattagat tattcttacc tctcatgatg catattttag caatcacctt atcattgtgt
     3481 ctcatgttct gtcctcctta tattctttgc ccagcaagat tctacttatg atgaatgaat
     3541 gctcttctcc ttttttcatt caatggtatg aagtatttgt tagggttctt tagtacttac
     3601 actttgttgt gtagaaaatg actgtaatgt ggtggtcagt gtattcttac tgtgattcag
     3661 agggaatcaa aagtagaaag caacagcacg tggtcctatc aaagatttgg ccatctctgc
     3721 ttcactgtca gcctcttaac tatatcttca cttactcaat ttggttttgt catgattttt
     3781 aaatgtagcc aatagatcaa ggttcttcca gtaaacacat atctgcataa atgcctcctt
     3841 gaagtcaata aagaaggaaa ttgagaagac tttaaattaa tgataattta gtttttaagt
     3901 acccacaaat aaatttttga aacattttct ttatttgaat acttagatgt catccaggaa
     3961 aatcactcaa taataattac ggcaaatctt taacccctca tttgggtagc ttaagataag
     4021 taatgccatt atgaatcaga attgattcat gactttagtt aagaaaatga aaaggaacat
     4081 ttcacgtatt tttaaaaatg atactaagga ataaagaagt acaactattg gaaaatatct
     4141 aagtatatga tttttaaatc ctccagtggc attaaatata tgattattag taattgttag
     4201 atagggtttt attcattcac aaatagaaga ctagcaagca tgtaactaac aaagttttta
     4261 caaaattgac tttgtggaat gctccaaatg tttggccatt ttgaggcaca aggtcagggg
     4321 tctctttatt gatagagctc cttctataat ttcccagcat acctgcctca cagttatctt
     4381 cctttcattg ttcactctct ttttcttctc aatgccatcc tgcctaggct cccatcatct
     4441 gcatctgaca cctttccttt ctttctttac tagtctcctt tgcgatgggt gtggctaagc
     4501 tctgtagagc cactcagaaa ctcattgttc cattctgtag ccagtaaaac atgcctccaa
     4561 agtgtcacag agtaattcta ctctctcttt taaattaggt ccaccggaaa tgttagtgaa
     4621 aggacattaa aaatgtgaca ggtgacatgt ttagctaaca tggatctgga gaaataggaa
     4681 gcagtagaat taaatgtttc cctttcaggt ttaattgtat ttgttcttgg gttttgtttt
     4741 atactgagtt ttaaatatat tctccaaata aaaacattat tttttctaac catatgtaga
     4801 gttaatctct ttgactaagt aattgaaaca aaagaacatt tgttcttttg tgactgcttt
     4861 tttcctaaaa cctgagccct cttttttttt tttgaaatta aagttgattt cctttttttt
     4921 tttttttttt tttttttttt tttttttttt tttgagacag agtctcgctc tgtcgcccag
     4981 gctggagtgc agtggcggga tctcggctca ctgcaagctc cgcctcccgg gttcacgcca
     5041 ttctcctgcc tcagcctccc aagtagctgg gactacaggc gcccgccact acgcccggct
     5101 aattttttgt atttttagta gagacggggt ttcaccgttt tagccgggat ggtctcgatc
     5161 tcctgacctc gtgatccgcc cgcctcggcc tcccaaagtg ctgggattac aggcgtgagc
     5221 caccgcgccc ggccttaaaa gttgatttcc ttcttcagta aggaaacctt tttataaatt
     5281 tgttttgcat tttaaaagtt ttactaatca atgatgagga aaaagatttg tcttcttgat
     5341 tttaaatagt ttcaggatca caggatgtaa tcagatgctt ccagtttatt tattttcagg
     5401 tattacacta gccatttaat cttttttatt tatttatttt cttcctgccc ctcggatggc
     5461 atataccagc catttagata ctaaactcta atagttaaac caatagttaa aattgtcctc
     5521 tctaaaacat tggctattta atataccagc ttaaatggcc tttctctcaa gtgagtcact
     5581 cttagtttaa gaaaattatg tgccttttta aaaaatatta tgaaatggta cttcatgaca
     5641 gaaacatttt atcagttata gtcttatttg attgaaaatt gttgagcatt tctgtaaaac
     5701 tttttacttt actaaatatt tcatctttcc tgtgactgtt ttctcaaaga atttaaaaga
     5761 ctcgatgtgt ctatgccaga atgtttctca tccttttgaa actgcctggg ccaggcgtag
     5821 tggctcacgc tgtaatccca gcacttttgg aggccaaggt gggcagatcg cgtgagccca
     5881 ggagtttgag accagcctgg acaacatggc gaaacggtgt ctctacagaa aaatttaaaa
     5941 attagccaag catagtggtg cacaactgta gccccagcca ctcgggaggc tgacgtggga
     6001 ggatcccttg aacctggggg cggaggctgc tgtgagcctt catcatgcca ctgcactcca
     6061 gcctgggcaa caaagcaaaa ccctgtctca aaaaaagaaa agaaaaaaag aaactgcttg
     6121 aaagtcatga cgaagaatgt caggagggga cttattctgg ctgcagttga ctttctcctt
     6181 aaatgtcaag tagtgattga tttggataag aagtaaactg ttacttttca taacatactt
     6241 taaggaattt atcaaattct atgtataatg cccattaaaa tatactccat tctggagtaa
     6301 agggtaagag taatattttt aaactagtta ataaagtctt tagctttcac ataaaccatg
     6361 atatttgagg tgtctaaaat cacagggtct tttttttttt tttcagtctt cccagttgtt
     6421 ctctgctcta ttcctaaata aagttaactt gaaaatgca
//
