LOCUS       NM_004332               1909 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1,
            mRNA.
ACCESSION   NM_004332
VERSION     NM_004332.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1909)
  AUTHORS   Marsillach,J., Suzuki,S.M., Richter,R.J., McDonald,M.G.,
            Rademacher,P.M., MacCoss,M.J., Hsieh,E.J., Rettie,A.E. and
            Furlong,C.E.
  TITLE     Human valacyclovir hydrolase/biphenyl hydrolase-like protein is a
            highly efficient homocysteine thiolactonase
  JOURNAL   PLoS One 9 (10), e110054 (2014)
   PUBMED   25333274
  REMARK    GeneRIF: The catalytic efficiency (kcat/Km) of rBPHL for
            homocysteine thiolactone hydrolysis was 7.7 x 10(4) M(-1)s(-1),
            orders of magnitude higher than that of PON1 or Blmh, indicating a
            more significant physiological role for BPHL in detoxifying HCTL
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1909)
  AUTHORS   Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., Malasky,M.,
            Sezgin,E., Kingsley,L.A., Goedert,J.J., Kirk,G.D., Gomperts,E.D.,
            Buchbinder,S.P., Troyer,J.L. and O'Brien,S.J.
  TITLE     Genetic variants in nuclear-encoded mitochondrial genes influence
            AIDS progression
  JOURNAL   PLoS One 5 (9), e12862 (2010)
   PUBMED   20877624
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1909)
  AUTHORS   Kim,I., Crippen,G.M. and Amidon,G.L.
  TITLE     Structure and specificity of a human valacyclovir activating
            enzyme: a homology model of BPHL
  JOURNAL   Mol Pharm 1 (6), 434-446 (2004)
   PUBMED   16028355
REFERENCE   6  (bases 1 to 1909)
  AUTHORS   Kim,I., Song,X., Vig,B.S., Mittal,S., Shin,H.C., Lorenzi,P.J. and
            Amidon,G.L.
  TITLE     A novel nucleoside prodrug-activating enzyme: substrate specificity
            of biphenyl hydrolase-like protein
  JOURNAL   Mol Pharm 1 (2), 117-127 (2004)
   PUBMED   15832508
  REMARK    GeneRIF: BPHL catalyzes the hydrolytic activation of the antiviral
            prodrugs valacyclovir and valganciclovir.
REFERENCE   7  (bases 1 to 1909)
  AUTHORS   Kim,I., Chu,X.Y., Kim,S., Provoda,C.J., Lee,K.D. and Amidon,G.L.
  TITLE     Identification of a human valacyclovirase: biphenyl hydrolase-like
            protein as valacyclovir hydrolase
  JOURNAL   J Biol Chem 278 (28), 25348-25356 (2003)
   PUBMED   12732646
  REMARK    GeneRIF: BPHL is a serine hydrolase that catalyzes the hydrolytic
            activation of amino acid ester prodrugs of nuceloside analogs such
            as valacyclovir and valganciclovir.
REFERENCE   8  (bases 1 to 1909)
  AUTHORS   Puente,X.S., Pendas,A.M. and Lopez-otin,C.
  TITLE     Structural characterization and chromosomal localization of the
            gene encoding human biphenyl hydrolase-related protein (BPHL)
  JOURNAL   Genomics 51 (3), 459-462 (1998)
   PUBMED   9721218
REFERENCE   9  (bases 1 to 1909)
  AUTHORS   Puente,X.S. and Lopez-Otin,C.
  TITLE     Cloning and expression analysis of a novel human serine hydrolase
            with sequence similarity to prokaryotic enzymes involved in the
            degradation of aromatic compounds
  JOURNAL   J Biol Chem 270 (21), 12926-12932 (1995)
   PUBMED   7759552
REFERENCE   10 (bases 1 to 1909)
  AUTHORS   Larocca,D., Peterson,J.A., Walkup,G., Urrea,R. and Ceriani,R.L.
  TITLE     Cloning and sequencing of a complementary DNA encoding a Mr 70,000
            human breast epithelial mucin-associated antigen
  JOURNAL   Cancer Res 50 (18), 5925-5930 (1990)
   PUBMED   2393862
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC037778.1, AL031963.40,
            BC106901.1, AJ617684.1 and BM977067.1.
            
            On Nov 25, 2018 this sequence version replaced NM_004332.3.
            
            Summary: This gene encodes a member of the serine protease family
            of hydrolytic enzymes which contain a serine in their active site.
            The encoded protein may play a role in activation of the antiviral
            prodrug valacyclovir. Alternatively spliced transcript variants
            have been described.[provided by RefSeq, Jan 2009].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.238312.1,
                                           SRR1660807.222525.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380379.10/ ENSP00000369739.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BC037778.1         341-342
            3-3                 AL031963.40        81107-81107
            4-137               BC037778.1         344-477
            138-461             BC037778.1         706-1029
            462-1000            BC106901.1         512-1050
            1001-1368           BC037778.1         1637-2004
            1369-1386           AJ617684.1         1352-1369
            1387-1526           BC037778.1         2024-2163
            1527-1529           BM977067.1         15-17               c
            1530-1909           AL031963.40        115593-115972
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p25.2"
     gene            1..1909
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /note="biphenyl hydrolase like"
                     /db_xref="GeneID:670"
                     /db_xref="HGNC:HGNC:1094"
                     /db_xref="MIM:603156"
     exon            1..137
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..906
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; valacyclovirase; breast epithelial
                     mucin-associated antigen; biphenyl hydrolase-related
                     protein; valacyclovir hydrolase; biphenyl hydrolase-like
                     (serine hydrolase)"
                     /codon_start=1
                     /product="valacyclovir hydrolase isoform 1 precursor"
                     /protein_id="NP_004323.2"
                     /db_xref="CCDS:CCDS4483.2"
                     /db_xref="GeneID:670"
                     /db_xref="HGNC:HGNC:1094"
                     /db_xref="MIM:603156"
                     /translation="MVAVLGGRGVLRLRLLLSALKPGIHVPRAGPAAAFGTSVTSAKV
                     AVNGVQLHYQQTGEGDHAVLLLPGMLGSGETDFGPQLKNLNKKLFTVVAWDPRGYGHS
                     RPPDRDFPADFFERDAKDAVDLMKALKFKKVSLLGWSDGGITALIAAAKYPSYIHKMV
                     IWGANAYVTDEDSMIYEGIRDVSKWSERTRKPLEALYGYDYFARTCEKWVDGIRQFKH
                     LPDGNICRHLLPRVQCPALIVHGEKDPLVPRFHADFIHKHVKGSRLHLMPEGKHNLHL
                     RFADEFNKLAEDFLQ"
     exon            138..241
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     exon            242..408
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     exon            409..562
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     exon            563..694
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     exon            695..818
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     exon            819..1909
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1396..1401
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /note="hexamer: AATAAA"
     polyA_site      1415
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
     regulatory      1510..1515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /note="hexamer: AATAAA"
     polyA_site      1529
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /note="major polyA site"
     regulatory      1883..1888
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
                     /note="hexamer: ATTAAA"
     polyA_site      1909
                     /gene="BPHL"
                     /gene_synonym="BPH-RP; MCNAA; VACVASE"
ORIGIN      
        1 gcactcccgg cagctacgcg acctgtgacc atggtggctg tgctgggcgg ccggggcgtg
       61 ttgcgcctgc ggctgcttct ctcagcgctg aagcccggga tccacgtccc acgggccgga
      121 cccgcggccg cgttcggcac ctcggtaacc tctgccaaag tggctgtgaa tggcgttcag
      181 ctgcattacc agcagactgg agagggagat cacgcagtcc tgctacttcc tgggatgtta
      241 ggaagtggag agactgattt tggacctcag ctcaagaacc tcaataagaa gctcttcacg
      301 gtggtcgcct gggatcctcg aggctatgga cattccaggc ccccagatcg cgatttccca
      361 gcagactttt ttgaaaggga tgcaaaagat gctgttgatt tgatgaaggc gctgaagttt
      421 aagaaggttt ctctgctggg gtggagtgat gggggcataa ccgcactcat tgctgctgca
      481 aaatatccat cttacatcca caagatggtg atctggggcg ccaacgccta cgtcactgac
      541 gaagacagca tgatatatga gggcatccga gatgtttcca aatggagtga gagaacaaga
      601 aagcctctag aagccctcta tgggtatgac tactttgcca gaacctgtga aaagtgggtg
      661 gatggcataa gacagtttaa acatctccca gatggtaaca tctgccggca cctgctgccc
      721 cgggtccagt gccccgcctt gattgtgcac ggtgagaagg atcctctggt cccacggttt
      781 catgccgact tcattcataa gcacgtgaaa ggctcacggc tgcatttgat gccagaaggc
      841 aaacacaacc tgcatttgcg ttttgcagat gaattcaaca agttagcaga agacttccta
      901 caatgagaat gcacactcca gtcttggtgg ttccttcgtg tggggcttga tcgtgttgct
      961 gcctgttaac atgatgcctt tgaaactctc cgcctttgaa actttctacc cctcccttca
     1021 atcttatcct aaccaaatga gaataatgac atattgaaaa cagcctctag cttcaggctg
     1081 ggcacggtgg ctcacagcta taatctcagc actttgggag gctgaggtgg gagaattgcc
     1141 tgagcccagg agttcaagac cagcttgtgc aatataggga gactccggct ctacaaaaaa
     1201 gagtttttca aaattagcca ggcgaagtgg cacacatctg tggtcccagg tgctcaggaa
     1261 gctgaggtgg gaggatcact tgagcccaat tcaaagctgc agtgagctgt aattgcatca
     1321 ctgcactcca acctgggcaa cagagtaaga ccttgtctta aaaaaaaata aaaacataaa
     1381 aaaaaataaa aaaggaataa agcctctaac ttcaatagct atgaaaatta aatccgattt
     1441 tctaagatga agttgtgaca agttatacca ttgtcttaac aggtgaattc ctgccataat
     1501 ttattttcaa ataaatttta ggtggagaat gtgtagtgtt aatgttggag attttaagac
     1561 atcttgaatt tgtactgtac cttttatctg agaggttcaa gcttcacttg tgtttttatt
     1621 tgtgtttatc tatcatattc aaagagaagt agggagagtc cgtgcccgag gtcctcaatt
     1681 ctaagatcag aggatgtcca gaacagcagc agataggggc aggtttgctt taccccatat
     1741 ccctctcctg gttcactttt gagagatgaa tactttgggc cagtaaaggt gaacaaaata
     1801 tgtattttca tcactctgta ctaaaaggac aggaatgtta ttaattaaaa cactaaagca
     1861 gaaatcctac attgctatta aaattaaact ttgattacca caataaaaa
//
