LOCUS       NM_032515               2626 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens BCL2 family apoptosis regulator BOK (BOK), mRNA.
ACCESSION   NM_032515 NM_014204
VERSION     NM_032515.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Beigl,T.B., Paul,A., Fellmeth,T.P., Nguyen,D., Barber,L.,
            Weller,S., Schafer,B., Gillissen,B.F., Aulitzky,W.E., Kopp,H.G.,
            Rehm,M., Andrews,D.W., Pluhackova,K. and Essmann,F.
  TITLE     BCL-2 and BOK regulate apoptosis by interaction of their C-terminal
            transmembrane domains
  JOURNAL   EMBO Rep 25 (9), 3896-3924 (2024)
   PUBMED   39048751
  REMARK    GeneRIF: BCL-2 and BOK regulate apoptosis by interaction of their
            C-terminal transmembrane domains.
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Meinhardt,A.L., Munkhbaatar,E., Hockendorf,U., Dietzen,M.,
            Dechant,M., Anton,M., Jacob,A., Steiger,K., Weichert,W., Brcic,L.,
            McGranahan,N., Branca,C., Kaufmann,T., Dengler,M.A. and Jost,P.J.
  TITLE     The BCL-2 family member BOK promotes KRAS-driven lung cancer
            progression in a p53-dependent manner
  JOURNAL   Oncogene 41 (9), 1376-1382 (2022)
   PUBMED   35091677
  REMARK    GeneRIF: The BCL-2 family member BOK promotes KRAS-driven lung
            cancer progression in a p53-dependent manner.
REFERENCE   3  (bases 1 to 2626)
  AUTHORS   Szczesniak,L.M., Bonzerato,C.G., Schulman,J.J., Bah,A. and
            Wojcikiewicz,R.J.H.
  TITLE     Bok binds to a largely disordered loop in the coupling domain of
            type 1 inositol 1,4,5-trisphosphate receptor
  JOURNAL   Biochem Biophys Res Commun 553, 180-186 (2021)
   PUBMED   33773141
  REMARK    GeneRIF: Bok binds to a largely disordered loop in the coupling
            domain of type 1 inositol 1,4,5-trisphosphate receptor.
REFERENCE   4  (bases 1 to 2626)
  AUTHORS   Shalaby,R., Flores-Romero,H. and Garcia-Saez,A.J.
  TITLE     The Mysteries around the BCL-2 Family Member BOK
  JOURNAL   Biomolecules 10 (12), 1638 (2020)
   PUBMED   33291826
  REMARK    GeneRIF: The Mysteries around the BCL-2 Family Member BOK.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2626)
  AUTHORS   Lucendo,E., Sancho,M., Lolicato,F., Javanainen,M., Kulig,W.,
            Leiva,D., Duart,G., Andreu-Fernandez,V., Mingarro,I. and Orzaez,M.
  TITLE     Mcl-1 and Bok transmembrane domains: Unexpected players in the
            modulation of apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 117 (45), 27980-27988 (2020)
   PUBMED   33093207
  REMARK    GeneRIF: Mcl-1 and Bok transmembrane domains: Unexpected players in
            the modulation of apoptosis.
REFERENCE   6  (bases 1 to 2626)
  AUTHORS   Zhang,H., Holzgreve,W. and De Geyter,C.
  TITLE     Evolutionarily conserved Bok proteins in the Bcl-2 family
  JOURNAL   FEBS Lett 480 (2-3), 311-313 (2000)
   PUBMED   11034351
REFERENCE   7  (bases 1 to 2626)
  AUTHORS   Zhang,H., Huang,Q., Ke,N., Matsuyama,S., Hammock,B., Godzik,A. and
            Reed,J.C.
  TITLE     Drosophila pro-apoptotic Bcl-2/Bax homologue reveals evolutionary
            conservation of cell death mechanisms
  JOURNAL   J Biol Chem 275 (35), 27303-27306 (2000)
   PUBMED   10811653
REFERENCE   8  (bases 1 to 2626)
  AUTHORS   Leo,C.P., Hsu,S.Y., Chun,S.Y., Bae,H.W. and Hsueh,A.J.
  TITLE     Characterization of the antiapoptotic Bcl-2 family member myeloid
            cell leukemia-1 (Mcl-1) and the stimulation of its message by
            gonadotropins in the rat ovary
  JOURNAL   Endocrinology 140 (12), 5469-5477 (1999)
   PUBMED   10579309
REFERENCE   9  (bases 1 to 2626)
  AUTHORS   Inohara,N., Ekhterae,D., Garcia,I., Carrio,R., Merino,J., Merry,A.,
            Chen,S. and Nunez,G.
  TITLE     Mtd, a novel Bcl-2 family member activates apoptosis in the absence
            of heterodimerization with Bcl-2 and Bcl-XL
  JOURNAL   J Biol Chem 273 (15), 8705-8710 (1998)
   PUBMED   9535847
REFERENCE   10 (bases 1 to 2626)
  AUTHORS   Hsu,S.Y., Kaipia,A., McGee,E., Lomeli,M. and Hsueh,A.J.
  TITLE     Bok is a pro-apoptotic Bcl-2 protein with restricted expression in
            reproductive tissues and heterodimerizes with selective
            anti-apoptotic Bcl-2 family members
  JOURNAL   Proc Natl Acad Sci U S A 94 (23), 12401-12406 (1997)
   PUBMED   9356461
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX460019.2, AF174487.1,
            CN279175.1, BP332256.1, CN279174.1, BX395656.2 and CD368092.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_032515.4.
            
            Summary: The protein encoded by this gene belongs to the BCL2
            family, members of which form homo- or heterodimers, and act as
            anti- or proapoptotic regulators that are involved in a wide
            variety of cellular processes. Studies in rat show that this
            protein has restricted expression in reproductive tissues,
            interacts strongly with some antiapoptotic BCL2 proteins, not at
            all with proapoptotic BCL2 proteins, and induces apoptosis in
            transfected cells. Thus, this protein represents a proapoptotic
            member of the BCL2 family. [provided by RefSeq, Sep 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.396210.1,
                                           SRR1660803.284028.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000318407.5/ ENSP00000314132.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-146               BX460019.2         15-160
            147-1027            AF174487.1         10-890
            1028-1394           CN279175.1         335-701
            1395-1709           BP332256.1         227-541
            1710-2122           CN279174.1         205-617
            2123-2564           BX395656.2         22-463              c
            2565-2626           CD368092.1         24-85               c
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q37.3"
     gene            1..2626
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /note="BCL2 family apoptosis regulator BOK"
                     /db_xref="GeneID:666"
                     /db_xref="HGNC:HGNC:1087"
                     /db_xref="MIM:605404"
     exon            1..249
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /inference="alignment:Splign:2.1.0"
     exon            250..498
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..917
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /note="hBOK; bcl2-L-9; bcl-2-like protein 9; BCL2 related
                     ovarian killer; BOK, BCL2 family apoptosis regulator"
                     /codon_start=1
                     /product="bcl-2-related ovarian killer protein"
                     /protein_id="NP_115904.1"
                     /db_xref="CCDS:CCDS2550.1"
                     /db_xref="GeneID:666"
                     /db_xref="HGNC:HGNC:1087"
                     /db_xref="MIM:605404"
                     /translation="MEVLRRSSVFAAEIMDAFDRSPTDKELVAQAKALGREYVHARLL
                     RAGLSWSAPERAAPVPGRLAEVCAVLLRLGDELEMIRPSVYRNVARQLHISLQSEPVV
                     TDAFLAVAGHIFSAGITWGKVVSLYAVAAGLAVDCVRQAQPAMVHALVDCLGEFVRKT
                     LATWLRRRGGWTDVLKCVVSTDPGLRSHWLVAALCSFGRFLKAAFFVLLPER"
     exon            499..627
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /inference="alignment:Splign:2.1.0"
     exon            628..791
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /inference="alignment:Splign:2.1.0"
     exon            792..1671
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..2626
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2594..2599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /note="hexamer: AATAAA"
     polyA_site      2626
                     /gene="BOK"
                     /gene_synonym="BCL2L9; BOKL"
                     /note="major polyA site"
ORIGIN      
        1 ctctcgccag ctgggagtcg cgcgctgccc acctcgctgc ccaggccccc gacgccgcgg
       61 caggagcccc ccaagagcgc gggaagcccc gtggacctgg cgctcccggc tcgggcgtgg
      121 acggggcggg cgccggggcg gggcgcgcgt cctcgcgggt ctgaatggaa gggtcgaggt
      181 cgtcgtcggc ggcgagcaga tcctgaagcc agaactccac cccggcgccc gcgccatgcg
      241 gcgggagagg tgcggcgccc cccacccgcg tcgccgccat ggaggtgctg cggcgctcct
      301 cggtcttcgc cgccgagatc atggacgcct ttgaccgctc gcccacagac aaggagctgg
      361 tggcccaggc caaggcgctg ggccgggagt acgtgcacgc gcggctgctg cgcgccggcc
      421 tctcctggag cgcgcccgag cgtgccgcgc cggtcccggg acgcctggct gaggtgtgcg
      481 cggtgctgct gcgcctgggc gatgagctgg agatgatccg gcccagcgtc taccgcaacg
      541 tggcgcgtca gctgcacatc tccctgcagt ctgagcctgt ggtgaccgat gcgttcctgg
      601 ccgtggctgg ccacatcttc tctgcaggca tcacgtgggg caaggtggtg tccctgtatg
      661 cggtggccgc ggggctggcc gtggactgtg tgaggcaggc ccagcctgcc atggtccacg
      721 ccctcgtgga ctgcctgggg gagttcgtgc gcaagaccct ggcaacctgg ctgcggagac
      781 gcggcggatg gactgatgtc ctcaagtgtg tggtcagcac agaccctggc ctccgctccc
      841 actggctggt ggctgcactc tgcagcttcg gccgcttcct gaaggctgcc ttcttcgtgc
      901 tgctgccaga gagatgagct gcccacctgg cagtggccgc agcctggccc tctgggccca
      961 acgcaggagg ccctcagcac ccgaacacat cttcctcctc cccacccgag cctggagcac
     1021 tctaaccctc ggagaccccc taagccccgt tcctccgcag acccaggccc tccggaaggg
     1081 gtgagtgggg aggggctttc ctgagcctgg agctgggctt tggggcagcc tgcgaccctc
     1141 cccgcttgtg tcccttctcc tgtgatctct gtgttttccc ttttctttct ggggccagga
     1201 agtcagggtc aactcccagg cctcagatgc aggggcccag aacacctgct ctcacctgag
     1261 ccccaggtga aggggcccgg gaacacctgc tctcacctga gccccaggtg aaggggcccg
     1321 ggaacacctg ctctcacctg aaccccaggt gaaggggccc ggaacacctg ctctcacctg
     1381 agccccaggt gaaggggccc ggaacacctg ctctcacctg agccccaggt gaaggggccc
     1441 gggaacacct gctctcacct gagccccagg tgaaggggcc cgggaacacc tgctctcacc
     1501 tgaaccccag gtgaaggggc ccagaacacc tgctctcacc tgagccccag gtgaaggggc
     1561 ccggaacacc tgctctcacc tgagccccag gtgaaggggc ccgggaacac ctgctctcac
     1621 ctgagcccct ggtgaagggg cccggaacac ttgctctcac ctgagcccca ggtgaagggg
     1681 cccggaacac ctgctctcac ctgagccccc ggtgaagggg cccggaacac ttgctctcac
     1741 ctgagcccca ggtgaagggg cccggaacac ctcctctcac ctgagcccca ggtgaagggg
     1801 cccggaacac ctcctgtcac ctgagcccca ggtgaagggg cccgggaaca cctctcacct
     1861 gaacccgggg gtcccatccc aggaagaagg gccatctcag gacatgagtc ctcaggggcc
     1921 ctgcacattc aatctgaagg tgaccctggc ctggctgaag ctggaagagc tgtggggact
     1981 cagcctgtaa acagagcgta aggttcacat gctggttgct taatccgttt ctggaggaag
     2041 agtatgacac ccacttgtga tggggtcctt gtgcggtggg gaccggggcc ggcgggctcc
     2101 aggccagcac acctaaccca tggatgtgga acctacggcc gagaaggaat gttgcatgag
     2161 tcggatccca gtccattgtc agtggagggt gagggtgacc ccatctgcta tttttgtgct
     2221 catcctcata caaccatttg gggatgtgcc tattagggct ccgtaagaac tcagatgcct
     2281 gggaagccca gcccctcagg tgcccccaca cacagccttc ccttgacgcc tacatttcta
     2341 ggcacatgtg aggcatcttt cctggagccc cgagccagcc ctgtccctcc ccagtgcagc
     2401 atggcactca ggagatacag gctggacatg gggcagtcgt tctggggagg cctggcctag
     2461 cagccaccca cctgagccct cccggccagg cttcgtgctg gggtgggcca tgtgccagga
     2521 caggagggtc ccggcggaaa gccagccccg gactcatcgt gacattgaga tcccactgga
     2581 gggtaggggt ggtaataaac ttctccaaac gatcgttgtc atttta
//
