LOCUS       NM_004330               6111 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens BCL2 interacting protein 2 (BNIP2), transcript variant
            2, mRNA.
ACCESSION   NM_004330
VERSION     NM_004330.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Wong,D.C.P., Xiao,J., Chew,T.W., Pan,M., Lee,C.J.M., Ang,J.W.,
            Yow,I., Thivakar,T., Ackers-Johnson,M., Lee,N.J.W., Foo,R.S.,
            Kanchanawong,P. and Low,B.C.
  TITLE     BNIP-2 Activation of Cellular Contractility Inactivates YAP for
            H9c2 Cardiomyoblast Differentiation
  JOURNAL   Adv Sci (Weinh) 9 (31), e2202834 (2022)
   PUBMED   35975420
  REMARK    GeneRIF: BNIP-2 Activation of Cellular Contractility Inactivates
            YAP for H9c2 Cardiomyoblast Differentiation.
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Pan,M., Chew,T.W., Wong,D.C.P., Xiao,J., Ong,H.T., Chin,J.F.L. and
            Low,B.C.
  TITLE     BNIP-2 retards breast cancer cell migration by coupling
            microtubule-mediated GEF-H1 and RhoA activation
  JOURNAL   Sci Adv 6 (31), eaaz1534 (2020)
   PUBMED   32789168
  REMARK    GeneRIF: BNIP-2 retards breast cancer cell migration by coupling
            microtubule-mediated GEF-H1 and RhoA activation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6111)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 6111)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6111)
  AUTHORS   Akamatsu,R., Ishida-Kitagawa,N., Aoyama,T., Oka,C. and Kawaichi,M.
  TITLE     BNIP-2 binds phosphatidylserine, localizes to vesicles, and is
            transported by kinesin-1
  JOURNAL   Genes Cells 20 (2), 135-152 (2015)
   PUBMED   25472445
  REMARK    GeneRIF: BNIP-2 is a kinesin-1 adapter involved in vesicular
            transportation in the cytoplasm and that association with cargos
            depends on interaction of the CRAL-TRIO domain with membrane
            phosphatidylserine.
REFERENCE   6  (bases 1 to 6111)
  AUTHORS   Low,B.C., Seow,K.T. and Guy,G.R.
  TITLE     The BNIP-2 and Cdc42GAP homology domain of BNIP-2 mediates its
            homophilic association and heterophilic interaction with Cdc42GAP
  JOURNAL   J Biol Chem 275 (48), 37742-37751 (2000)
   PUBMED   10954711
REFERENCE   7  (bases 1 to 6111)
  AUTHORS   Low,B.C., Seow,K.T. and Guy,G.R.
  TITLE     Evidence for a novel Cdc42GAP domain at the carboxyl terminus of
            BNIP-2
  JOURNAL   J Biol Chem 275 (19), 14415-14422 (2000)
   PUBMED   10799524
REFERENCE   8  (bases 1 to 6111)
  AUTHORS   Low,B.C., Lim,Y.P., Lim,J., Wong,E.S. and Guy,G.R.
  TITLE     Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by
            fibroblast growth factor receptor-1 prevents its binding to
            Cdc42GAP and Cdc42
  JOURNAL   J Biol Chem 274 (46), 33123-33130 (1999)
   PUBMED   10551883
REFERENCE   9  (bases 1 to 6111)
  AUTHORS   Ohi,N., Tokunaga,A., Tsunoda,H., Nakano,K., Haraguchi,K., Oda,K.,
            Motoyama,N. and Nakajima,T.
  TITLE     A novel adenovirus E1B19K-binding protein B5 inhibits apoptosis
            induced by Nip3 by forming a heterodimer through the C-terminal
            hydrophobic region
  JOURNAL   Cell Death Differ 6 (4), 314-325 (1999)
   PUBMED   10381623
REFERENCE   10 (bases 1 to 6111)
  AUTHORS   Boyd,J.M., Malstrom,S., Subramanian,T., Venkatesh,L.K.,
            Schaeper,U., Elangovan,B., D'Sa-Eipper,C. and Chinnadurai,G.
  TITLE     Adenovirus E1B 19 kDa and Bcl-2 proteins interact with a common set
            of cellular proteins
  JOURNAL   Cell 79 (2), 341-351 (1994)
   PUBMED   7954800
  REMARK    Erratum:[Cell. 1994 Dec 16;79(6):1121.. PMID: 8001138]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC092755.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jan 10, 2019 this sequence version replaced NM_004330.3.
            
            Summary: This gene is a member of the BCL2/adenovirus E1B 19
            kd-interacting protein (BNIP) family. It interacts with the E1B 19
            kDa protein, which protects cells from virally-induced cell death.
            The encoded protein also interacts with E1B 19 kDa-like sequences
            of BCL2, another apoptotic protector. Alternate splicing results in
            multiple transcript variants. [provided by RefSeq, Mar 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.3037726.1,
                                           SRR7346977.2413730.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000607373.6/ ENSP00000475320.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               AC092755.4         111169-111354       c
            187-293             AC092755.4         104442-104548       c
            294-361             AC092755.4         102275-102342       c
            362-538             AC092755.4         101626-101802       c
            539-715             AC092755.4         99945-100121        c
            716-818             AC092755.4         94671-94773         c
            819-950             AC092755.4         93217-93348         c
            951-1037            AC092755.4         91310-91396         c
            1038-1136           AC092755.4         90926-91024         c
            1137-6111           AC092755.4         81180-86154         c
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q22.2"
     gene            1..6111
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /note="BCL2 interacting protein 2"
                     /db_xref="GeneID:663"
                     /db_xref="HGNC:HGNC:1083"
                     /db_xref="MIM:603292"
     exon            1..186
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            187..293
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..1188
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     BCL2/adenovirus E1B 19 kDa protein-interacting protein 2;
                     BCL2/adenovirus E1B 19kDa interacting protein 2"
                     /codon_start=1
                     /product="BCL2/adenovirus E1B 19 kDa protein-interacting
                     protein 2 isoform 2"
                     /protein_id="NP_004321.3"
                     /db_xref="CCDS:CCDS10174.3"
                     /db_xref="GeneID:663"
                     /db_xref="HGNC:HGNC:1083"
                     /db_xref="MIM:603292"
                     /translation="MEGVELKEEWQDEDFPIPLPEDDSIEADILAITGPEDQPGSLEV
                     NGNKVRKKLMAPDISLTLDPSDGSVLSDDLDESGEIDLDGLDTPSENSNEFEWEDDLP
                     KPKTTEVIRKGSITEYTAAEEKEDGRRWRMFRIGEQDHRVDMKAIEPYKKVISHGGYY
                     GDGLNAIVVFAVCFMPESSQPNYRYLMDNLFKYVIGTLELLVAENYMIVYLNGATTRR
                     KMPSLGWLRKCYQQIDRRLRKNLKSLIIVHPSWFIRTLLAVTRPFISSKFSQKIRYVF
                     NLAELAELVPMEYVGIPECIKQVDQELNGKQDEPKNEQ"
     exon            294..361
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            362..538
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            539..715
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            716..818
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            819..950
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1037
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1136
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1137..6111
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6092..6097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /note="hexamer: AATAAA"
     polyA_site      6111
                     /gene="BNIP2"
                     /gene_synonym="BNIP-2; NIP2"
                     /note="major polyA site"
ORIGIN      
        1 actgtgtccg ggtcagctgc tgccgccgac gccgtaccgc tgcggccggg ggattgggcc
       61 ggggtctcca ccgccgaccg aggggagcgg gctccgctcg gccctgcttt ttgcgacctg
      121 gccgtcagcc ccacgtcgcc ggcctggagg ggcgaagagg acgagggggc caaggcttcc
      181 tccggggaca ttggctccct ggattatcaa gagtttgtag ttgacattga atccaggctg
      241 aggatggaag gtgtggaact taaagaagaa tggcaagatg aagattttcc gataccttta
      301 ccagaagatg atagtattga agcagatata ctagctataa ctggaccaga ggaccagcct
      361 ggctcactag aagttaatgg aaataaagtg agaaagaaac taatggctcc agacattagc
      421 ctgacactgg atcctagtga tggctctgta ttgtcagatg atttggatga aagtggggag
      481 attgacttag atggcttaga cacaccgtca gagaatagta atgagtttga gtgggaagat
      541 gatcttccaa aacccaagac tactgaagta attaggaaag gctcaattac tgaatacaca
      601 gcagcagagg aaaaagaaga tggacgacgc tggcgtatgt tcaggattgg agaacaggac
      661 cacagggttg atatgaaggc aattgaaccc tataaaaaag ttatcagcca tgggggatat
      721 tatggggatg gattaaatgc cattgttgtg tttgctgtct gtttcatgcc tgaaagtagt
      781 cagcctaact atagatacct gatggacaat ctttttaaat atgttattgg cactttggag
      841 ctattagtag cagaaaacta catgatagtt tatttaaatg gtgcaacaac tcgaagaaaa
      901 atgcccagtc tgggatggct caggaaatgt tatcagcaaa ttgatagaag gttacggaaa
      961 aatctaaaat ccctaatcat tgtacatcct tcttggttta tcagaacact tctggctgtt
     1021 acaagaccat ttattagctc gaaattcagc caaaaaatta gatacgtgtt taatttggca
     1081 gaactagcag aacttgtccc catggaatac gttggcatac cagaatgcat aaaacaagtt
     1141 gatcaagaac ttaatggaaa acaagatgaa ccgaaaaatg aacagtaagt ttggcatcta
     1201 gtccaaacaa gactgaagaa tgtgctgatg gagcagtgct gtttctgcat tcataatgca
     1261 tttattggcc catattttta tgtaacctgt tacaaaatag acttgacttt ttcataatgg
     1321 acttttgtat tatacaaggg actgttcact gctgtactgg tttgcaaatt tcttgaattt
     1381 agctctttaa tagctaactg tattattatc gttttatatt ttatattgct aaatagagaa
     1441 ccacacttta tataaagtag tttttgcatt tgtttattga atgatgcatc ttcttcggtg
     1501 aaatatttat atgcataaat ggcaaaggaa agaaataata tatattttta tgtcattgag
     1561 caatattttt taatgtgtac ctgtcttatg gaagaaatat gcaggtatat aagaccacga
     1621 ttttctaaag tgcatattag aatttttgtt tttgtaaatg gttaaataca tttcctgggt
     1681 aacttaggaa attaagtttt tcataaggca acagatggtt aaactgattg tcatgaatac
     1741 ccaaagatca tgtatataat cgaagtgtat tagtaccatc ccaaggtttt tttctcattt
     1801 aacatatttg tttcataatt cagcaagtac agatgcaagc gcattgcaca ctttttcctt
     1861 tctaaactta aagacaagtc aaaaagccat tcttagaact agaggattta agagggtagg
     1921 aattagggtt tgtatatatg tatatatggg acattttatc ttctggccca aagtcagaac
     1981 tttataaaaa tcttgagttt gttcactaat gtgaaataag ctatgtgtcc agggtattgc
     2041 tcccctgagt gtatatgagt gctgagtagt attgcagaga atgtgatgag ttattcactg
     2101 tcacaacttt ttctatagaa aacaggggct gctttttaaa ctctcactat gggcacttta
     2161 ccaaaatact tccatatcaa ttatttgaac tcggtagttg tttgacctag ttagatgtgg
     2221 tgtttattca agtttgtatg aaatcatgtt tgacaatact gtaaattagg ttaattttgt
     2281 aagtcttagc atcatcatat tgtgctgttt tggataacac gtttgttaca agcatttaaa
     2341 ctgtttcatt tggtagtacc tttacattga aataaattgt gtttgtgcct ccaaacaaaa
     2401 agtttaatta ccgacaaaat tatttctaaa acaacagatt gatctgatat tgtgtcatat
     2461 acttgaaagg ctcatacaca gctggtaagc ataatacctt ccaaatttga aggtcatggc
     2521 tactcttcaa cccttcctgt aaggtatttt acaaaacagt ggcctagagt agcaaaattg
     2581 aagtggatgt gagctaacct ttcaagattt agtctatcct agattgaaca cacatgtggc
     2641 tgtgaccatt ctcatgtact tacaaacctt ctgttacaaa agggaacttt cctatatttt
     2701 ttgtcctcct cagtcctaaa cttggcatgc taacttaaaa ccagttatag gagagagatg
     2761 ctggaggatt cccaggatga aacattcctc agcagcctcc tgacttaact cagatccagc
     2821 agctcttggc agatcctgaa ctaatgtcat tacagtgaat tgagccaaaa cgattattgc
     2881 ttgctcacat tttaggaaca cttgttgttg aagaagcaaa ctaacaataa caagttaggc
     2941 attccttctg aatttaccat ataaggatta tgtataaaca attatattga atttgtcatt
     3001 agattctata tttgtagacg atttaacgta gtctttcatc tgtacatctt tatcttgctc
     3061 aaaatgggct gaggttttta ataggtggtt ttctaccaca ttaagtactt ggttcttcag
     3121 tttaaaaaat atgaagaata acttcaactc ttttttgcat tattttgatt tttgattttg
     3181 cagtcattgc tcctaactgt agatacatgt tgcaaaagtg atgtttttct gaattttttt
     3241 taaaagtaga agactattct gttaaacatt aaaattcaga gacaacagtg ataacacctg
     3301 ggatataatg aaaatgaggt gctgtgaatc agtaaactaa agggatacct gctttaacct
     3361 gcaccttaat ggaatctggt atagagatgt acccacaata aatgttttat aagatttaag
     3421 atcataatag taggttagag gaagagcatt tattaagtct gttgtttcta atctatagct
     3481 aagttaacat ttcccatatt attccatttt aatttcccat tactgaccaa ggtgaaagta
     3541 tatgtgattg tacttttatc tttctgttgg gatcatggat ctaatttaac tggcttaatt
     3601 tgatgcttct gtcactaggc tctaagtgcc ccttttttag tcagaagttt ttcatgtgaa
     3661 ataatttgat tctgtgttct gcttgatcat actcctaagc aagcaccaag ggaagacgtt
     3721 ctattcaccc cacaatttaa gcttaacaat ctttaactca gagggagata tgaagaaata
     3781 tgattctaag tagggtctag cagaggcatt ggaaagtgat aaaagtagga agtaagagaa
     3841 agcaaacctt tataaagaga taaagacata gttgttacct aagatgccac tgtatgtgtg
     3901 ccctctcttt tttttttttt ttttttggag acagagactc actctgtcac ccattctgga
     3961 gtgcagtggc gcaatcctgg ctcactgcaa cctctgcctc ccgggttcaa gcatttctcc
     4021 tgcctcagcc tcctgagtag ctgggattac aggcacacgc cacaatgccc ggctaatttt
     4081 tgtattttta gtagagacgg ggtttcgcca tgctaggctg gtctcgaact cctgacctca
     4141 tgatctgcct gtctcagcct cccaaatgct tggattacag gcttgagcca ccacgcccgg
     4201 ccatgtgtgt ccttttaagg gaagtaccat atgtgactgc tgatggtaac atttcttgta
     4261 tgcagcagga aaacctgcag gctatacgca aggtatgtga ataaagactg tggattgaag
     4321 ggggaaaagt tgataataca tacccatgat aagaattcat attttctctt tctttgtctc
     4381 ttcatacaga agcaagttcg agcagagaag gttagcagtt acagcactta aaagtttagg
     4441 attgagggtt tttggttggt ttttttgttt tttcatattt cactgttgct gaacattgat
     4501 ttagtcttcc aaaatggaca tcactagtaa gtgcactgaa tactgaaata tgctgattgg
     4561 caagcatata tctgtagcta gaattttttt aaccgtaatt ccagaattta aaaaggtaag
     4621 agatctgctt tttgatagag gccttttaaa aagactatat aacatttcta tttggctgtt
     4681 tatgtgaatg ctcagggaat ttggacatgt gttttataca gaagtggctt tttatatttc
     4741 ttcaagtgat aaattagcat taggacatat cagatagaac aggccagttt cctgatgaga
     4801 ttggtagaga tgttacggta ggttcatcct tcctttttcc atgttatatg tggagaagtg
     4861 ttaaacttgc tcaagcaaat gggagtagga gataggctga aaatagagat tgctacacca
     4921 ttctattttg ttttcatgcc tgttttttct ccaatcctat atggacattt gagaaatgga
     4981 ccacaaatct gagagggata gagtaaaaag aggcattaat gtatgtactc acaagttaga
     5041 cctattttgc tgttaaaata ggtctggtct tgttataggt cataggaatt gtgtagtttg
     5101 agttgctgcg agaaagagtc aagaacttag acttgaatgg gaaggccaac tggtgaggga
     5161 agtggggtat tatctactca aatgttctaa gctaatacaa aagaattacc tggcaagcat
     5221 ccccctctgc ccttcttgaa attctgaatg aacttagaat cagatcaatg gtgagaccca
     5281 gttatgtaac tttcctttga agttctaaag gataattgaa aataaagtag tctggcatgt
     5341 ttaacagtaa ggagtgacac taaaatttga atataacagt atgttgcaac tatttataat
     5401 agacctattt atgttttgag aactaagtaa aatgctatga aaaattcaca taaagcacgt
     5461 aagcaccaaa tgaaaatcat ttcgtgcttt caaattaatg atactaggct tgtaggatgt
     5521 cttagaatca tgtttaacaa gaagttaaaa tatttgcatt ttgaaatttt tatgcagttt
     5581 ggctaaggag gaacttggaa aataacattc atcagcttct acactgcact ttttgtatgt
     5641 ggactttgta aagtttaatg tccgtttaac agttgcttgt ttatttgtgt aaacctagct
     5701 agtaaatatt gatgatgttc agggccactg acttgcacag cccttggagc actattcaca
     5761 cagaacacaa ttcagagttt gttaagacat taacacactt tgatatctat tccaaaaata
     5821 cttttgttac cacaggttca ttgctgtcat ttagacaaaa ttaatgtact gtgatatttt
     5881 cctactctta agatgatgtt gcccagccga gtactgtcag aaattcagtt ctgatgtctg
     5941 tgtgtgcaag atattaatgt ggtccctgta agaaatacca gagttggtca tgatttacta
     6001 aggagatgta atattttctt tgacctgtta ggaatataac ttgtgtcttg atttgttgca
     6061 catcatattt tttgcaacat taatgaattt aaataaatta attggaacgt t
//
