LOCUS       NM_017637              12808 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens basonuclin zinc finger protein 2 (BNC2), transcript
            variant 1, mRNA.
ACCESSION   NM_017637
VERSION     NM_017637.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Orang,A., Dredge,B.K., Liu,C.Y., Bracken,J.M., Chen,C.H.,
            Sourdin,L., Whitfield,H.J., Lumb,R., Boyle,S.T., Davis,M.J.,
            Samuel,M.S., Gregory,P.A., Khew-Goodall,Y., Goodall,G.J.,
            Pillman,K.A. and Bracken,C.P.
  TITLE     Basonuclin-2 regulates extracellular matrix production and
            degradation
  JOURNAL   Life Sci Alliance 6 (10), e202301984 (2023)
   PUBMED   37536977
  REMARK    GeneRIF: Basonuclin-2 regulates extracellular matrix production and
            degradation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Bobowski-Gerard,M., Boulet,C., Zummo,F.P., Dubois-Chevalier,J.,
            Gheeraert,C., Bou Saleh,M., Strub,J.M., Farce,A., Ploton,M.,
            Guille,L., Vandel,J., Bongiovanni,A., Very,N., Woitrain,E.,
            Deprince,A., Lalloyer,F., Bauge,E., Ferri,L., Ntandja-Wandji,L.C.,
            Cotte,A.K., Grangette,C., Vallez,E., Cianferani,S., Raverdy,V.,
            Caiazzo,R., Gnemmi,V., Leteurtre,E., Pourcet,B., Paumelle,R.,
            Ravnskjaer,K., Lassailly,G., Haas,J.T., Mathurin,P., Pattou,F.,
            Dubuquoy,L., Staels,B., Lefebvre,P. and Eeckhoute,J.
  TITLE     Functional genomics uncovers the transcription factor BNC2 as
            required for myofibroblastic activation in fibrosis
  JOURNAL   Nat Commun 13 (1), 5324 (2022)
   PUBMED   36088459
  REMARK    GeneRIF: Functional genomics uncovers the transcription factor BNC2
            as required for myofibroblastic activation in fibrosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12808)
  AUTHORS   Makki,N., Zhao,J., Liu,Z., Eckalbar,W.L., Ushiki,A.,
            Khanshour,A.M., Wu,J., Rios,J., Gray,R.S., Wise,C.A. and Ahituv,N.
  TITLE     Genomic characterization of the adolescent idiopathic
            scoliosis-associated transcriptome and regulome
  JOURNAL   Hum Mol Genet 29 (22), 3606-3615 (2021)
   PUBMED   33179741
  REMARK    GeneRIF: Genomic characterization of the adolescent idiopathic
            scoliosis-associated transcriptome and regulome.
REFERENCE   4  (bases 1 to 12808)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 12808)
  AUTHORS   Ogura,Y., Kou,I., Miura,S., Takahashi,A., Xu,L., Takeda,K.,
            Takahashi,Y., Kono,K., Kawakami,N., Uno,K., Ito,M., Minami,S.,
            Yonezawa,I., Yanagida,H., Taneichi,H., Zhu,Z., Tsuji,T., Suzuki,T.,
            Sudo,H., Kotani,T., Watanabe,K., Hosogane,N., Okada,E., Iida,A.,
            Nakajima,M., Sudo,A., Chiba,K., Hiraki,Y., Toyama,Y., Qiu,Y.,
            Shukunami,C., Kamatani,Y., Kubo,M., Matsumoto,M. and Ikegawa,S.
  TITLE     A Functional SNP in BNC2 Is Associated with Adolescent Idiopathic
            Scoliosis
  JOURNAL   Am J Hum Genet 97 (2), 337-342 (2015)
   PUBMED   26211971
  REMARK    GeneRIF: A functional SNP leading to increased expression in BNC2
            is implicated in the etiology of Adolescent Idiopathic Scoliosis.
REFERENCE   6  (bases 1 to 12808)
  AUTHORS   Jacobs,L.C., Hamer,M.A., Gunn,D.A., Deelen,J., Lall,J.S., van
            Heemst,D., Uh,H.W., Hofman,A., Uitterlinden,A.G., Griffiths,C.E.M.,
            Beekman,M., Slagboom,P.E., Kayser,M., Liu,F. and Nijsten,T.
  TITLE     A Genome-Wide Association Study Identifies the Skin Color Genes
            IRF4, MC1R, ASIP, and BNC2 Influencing Facial Pigmented Spots
  JOURNAL   J Invest Dermatol 135 (7), 1735-1742 (2015)
   PUBMED   25705849
  REMARK    GeneRIF: BNC2 gene mutation influencing facial pigmented spots.
REFERENCE   7  (bases 1 to 12808)
  AUTHORS   Vanhoutteghem,A. and Djian,P.
  TITLE     The human basonuclin 2 gene has the potential to generate nearly
            90,000 mRNA isoforms encoding over 2000 different proteins
  JOURNAL   Genomics 89 (1), 44-58 (2007)
   PUBMED   16942855
  REMARK    GeneRIF: transcripts are initiated from six promoters, are
            alternatively spliced at multiple positions, and are polyadenylated
            at four sites
REFERENCE   8  (bases 1 to 12808)
  AUTHORS   Vanhoutteghem,A. and Djian,P.
  TITLE     Basonuclins 1 and 2, whose genes share a common origin, are
            proteins with widely different properties and functions
  JOURNAL   Proc Natl Acad Sci U S A 103 (33), 12423-12428 (2006)
   PUBMED   16891417
  REMARK    GeneRIF: Data show that basonuclin (Bn)2 but not bn1 colocalizes
            with SC35 in nuclear speckles, and may have a function in nuclear
            processing of mRNA.
REFERENCE   9  (bases 1 to 12808)
  AUTHORS   Romano,R.A., Li,H., Tummala,R., Maul,R. and Sinha,S.
  TITLE     Identification of Basonuclin2, a DNA-binding zinc-finger protein
            expressed in germ tissues and skin keratinocytes
  JOURNAL   Genomics 83 (5), 821-833 (2004)
   PUBMED   15081112
  REMARK    GeneRIF: molecular cloning
REFERENCE   10 (bases 1 to 12808)
  AUTHORS   Vanhoutteghem,A. and Djian,P.
  TITLE     Basonuclin 2: an extremely conserved homolog of the zinc finger
            protein basonuclin
  JOURNAL   Proc Natl Acad Sci U S A 101 (10), 3468-3473 (2004)
   PUBMED   14988505
  REMARK    GeneRIF: the extreme conservation of the basonuclin 2 amino acid
            sequence across vertebrates suggests that basonuclin 2 serves an
            important function, presumably as a regulatory protein of DNA
            transcription
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA494074.1, AK131398.1,
            AY438376.1 and AL449983.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_017637.5.
            
            Summary: This gene encodes a conserved zinc finger protein. The
            encoded protein functions in skin color saturation. Mutations in
            this gene are associated with facial pigmented spots. This gene is
            also associated with susceptibility to adolescent idiopathic
            scoliosis. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1966682, SAMEA1968189
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380672.9/ ENSP00000370047.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                DA494074.1         3-28
            27-2496             AK131398.1         27-2496
            2497-3731           AY438376.1         2260-3494
            3732-12808          AL449983.2         178354-187430
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p22.3-p22.2"
     gene            1..12808
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /note="basonuclin zinc finger protein 2"
                     /db_xref="GeneID:54796"
                     /db_xref="HGNC:HGNC:30988"
                     /db_xref="MIM:608669"
     exon            1..25
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..3322
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger protein basonuclin-2; basonuclin 2"
                     /codon_start=1
                     /product="zinc finger protein basonuclin-2 isoform 1"
                     /protein_id="NP_060107.3"
                     /db_xref="CCDS:CCDS6482.2"
                     /db_xref="GeneID:54796"
                     /db_xref="HGNC:HGNC:30988"
                     /db_xref="MIM:608669"
                     /translation="MAHLGPTPPPHSLNYKSEDRLSEQDWPAYFKVPCCGVDTSQIES
                     EEAEVDVRERETQRDREPKRARDLTLRDSCTDNSMQFGTRTTTAEPGFMGTWQNADTN
                     LLFRMSQQAIRCTLVNCTCECFQPGKINLRTCDQCKHGWVAHALDKLSTQHLYHPTQV
                     EIVQSNVVFDISSLMLYGTQAVPVRLKILLDRLFSVLKQEEVLHILHGLGWTLRDYVR
                     GYILQDAAGKVLDRWAIMSREEEIITLQQFLRFGETKSIVELMAIQEKEGQAVAVPSS
                     KTDSDIRTFIESNNRTRSPSLLAHLENSNPSSIHHFENIPNSLAFLLPFQYINPVSAP
                     LLGLPPNGLLLEQPGLRLREPSLSTQNEYNESSESEVSPTPYKNDQTPNRNALTSITN
                     VEPKTEPACVSPIQNSAPVSDLTKTEHPKSSFRIHRMRRMGSASRKGRVFCNACGKTF
                     YDKGTLKIHYNAVHLKIKHRCTIEGCNMVFSSLRSRNRHSANPNPRLHMPMLRNNRDK
                     DLIRATSGAATPVIASTKSNLALTSPGRPPMGFTTPPLDPVLQNPLPSQLVFSGLKTV
                     QPVPPFYRSLLTPGEMVSPPTSLPTSPIIPTSGTIEQHPPPPSEPVVPAVMMATHEPS
                     ADLAPKKKPRKSSMPVKIEKEIIDTADEFDDEDDDPNDGGAVVNDMSHDNHCHSQEEM
                     SPGMSVKDFSKHNRTRCISRTEIRRADSMTSEDQEPERDYENESESSEPKLGEESMEG
                     DEHIHSEVSEKVLMNSERPDENHSEPSHQDVIKVKEEFTDPTYDMFYMSQYGLYNGGG
                     ASMAALHESFTSSLNYGSPQKFSPEGDLCSSPDPKICYVCKKSFKSSYSVKLHYRNVH
                     LKEMHVCTVAGCNAAFPSRRSRDRHSANINLHRKLLTKELDDMGLDSSQPSLSKDLRD
                     EFLVKIYGAQHPMGLDVREDASSPAGTEDSHLNGYGRGMAEDYMVLDLSTTSSLQSSS
                     SIHSSRESDAGSDEGILLDDIDGASDSGESAHKAEAPALPGSLGAEVSGSLMFSSLSG
                     SNGGIMCNICHKMYSNKGTLRVHYKTVHLREMHKCKVPGCNMMFSSVRSRNRHSQNPN
                     LHKNIPFTSVD"
     exon            26..151
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     exon            152..352
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     exon            353..455
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     exon            456..691
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     exon            692..2661
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     exon            2662..12808
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5886..5891
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /note="hexamer: AATGAA"
     polyA_site      5907
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /note="major polyA site"
     regulatory      12786..12791
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
                     /note="hexamer: ATTAAA"
     polyA_site      12808
                     /gene="BNC2"
                     /gene_synonym="bn2; BSN2; LUTO"
ORIGIN      
        1 acttgacaac cagcatgccg agatggcaca ccttgggccc accccacctc cacatagcct
       61 taattacaaa tcagaggaca ggcttagtga gcaagactgg ccagcatatt tcaaggtccc
      121 atgttgtggg gttgatacat ctcaaattga gtcagaagag gcagaagtgg atgtgagaga
      181 aagagagaca cagagagaca gagagccaaa gagggcaaga gacttgactt taagagactc
      241 ctgtactgac aactccatgc agttcggaac cagaacgact acggctgaac cagggttcat
      301 ggggacatgg caaaacgctg atactaacct cttattcaga atgtcccaac aggccatccg
      361 ttgcacactg gtaaactgca catgtgaatg ttttcagcca gggaagatta acctgaggac
      421 ttgtgatcag tgtaaacatg gctgggtggc acatgccttg gataagctca gcacgcagca
      481 cctgtaccac cccacccaag tggagattgt gcagtccaac gtcgtgtttg acatcagcag
      541 cctgatgctc tatgggacac aagcagtgcc tgtgcggcta aagatcctgc tggaccgtct
      601 cttcagcgtc ctgaagcaag aggaggtact gcacatactg cacggccttg gctggactct
      661 gcgggactat gtccgaggat acatccttca ggatgctgct ggcaaggtgc tggaccgctg
      721 ggccatcatg tctcgagaag aggaaatcat cacccttcag cagtttctgc ggtttggaga
      781 aaccaaatcc attgtggagc tgatggcaat tcaggagaaa gaagggcagg ccgtggctgt
      841 accatcttca aagacagact cagatataag gactttcatt gagagcaata atcgcaccag
      901 gagtcccagc ctccttgctc acttagagaa cagcaatcct tccagcattc atcacttcga
      961 aaacatccca aacagccttg catttctgct tccattccag tacataaacc ctgtctcagc
     1021 accactgcta gggttgcctc caaatgggct actgttagag caaccagggt tgaggctgcg
     1081 ggaacccagc ctttcaactc agaatgaata taatgagagc agcgaatccg aagtttctcc
     1141 cacaccttat aagaatgatc aaacacccaa tagaaatgcc ctgaccagca ttactaatgt
     1201 ggagcccaaa accgagccag cctgtgtctc tcccattcag aattctgccc cagtcagtga
     1261 tctaaccaaa actgaacacc caaaaagctc attccggatt catcggatga gaaggatggg
     1321 gtcagcctct aggaaaggaa gagtgttctg taatgcatgt gggaagacat tctatgacaa
     1381 aggtactctc aaaattcatt acaatgctgt tcacctgaag atcaaacatc gatgcaccat
     1441 tgaaggttgc aacatggtct ttagctccct ccgaagtcgt aatcgccaca gtgcaaaccc
     1501 caatcctcgc cttcacatgc ctatgctaag gaataaccga gataaagatt taattcgggc
     1561 cacctcagga gctgccaccc ctgtcatagc aagtacaaaa tcaaatctgg cactcacaag
     1621 ccctggccga cccccaatgg gttttaccac tccccctcta gaccctgtct tgcaaaatcc
     1681 tctccctagc cagctagtat tttctgggct aaagactgta caaccagttc ctccatttta
     1741 tagaagttta ctcactccag gggaaatggt gagtcctcca acctccctcc caaccagtcc
     1801 catcattcca accagtggta ccatagagca gcaccccccg ccaccctctg agccagtagt
     1861 gccagcagtg atgatggcca cccatgagcc cagtgctgac ctggcaccca agaaaaagcc
     1921 caggaagtca agcatgcctg tgaagattga gaaggaaatt attgataccg ccgatgagtt
     1981 tgatgatgaa gatgatgacc ccaatgatgg tggagctgtg gtcaatgaca tgagccatga
     2041 caatcattgt cactcccaag aggagatgag cccaggcatg tctgtgaagg acttttctaa
     2101 gcataacagg acccggtgca tttcaaggac tgaaataagg agggccgaca gcatgacttc
     2161 tgaagaccaa gaacctgagc gggactatga gaacgagtct gagtcttcgg agcccaaact
     2221 gggcgaggaa tccatggaag gggatgagca cattcacagc gaagtgagtg aaaaagtcct
     2281 gatgaatagt gagaggcctg atgagaacca cagtgagccc tctcaccagg acgtcatcaa
     2341 ggtgaaggaa gaatttacag accccactta cgacatgttt tacatgagcc agtatggact
     2401 gtacaatggt gggggtgcca gcatggccgc cttgcatgag agctttacat cgtctctgaa
     2461 ttatggcagc cctcaaaagt tctccccaga aggtgaccta tgttctagcc cagaccccaa
     2521 aatctgttat gtgtgcaaga agagtttcaa aagctcctac agtgtgaaac ttcactacag
     2581 gaacgttcac ttgaaagaga tgcacgtctg cacagtggct ggttgcaatg ctgcattccc
     2641 ctctcgccga agccgagaca gacacagtgc caacataaac ctacatcgta aactgttgac
     2701 caaagaactc gatgacatgg gcctggactc gtcgcagccc tcccttagca aggacctccg
     2761 cgatgaattt ttggtgaaga tatatggtgc ccagcacccc atggggctcg atgtcaggga
     2821 agacgcctcc tctcccgcag ggactgaaga ctcccacctg aacgggtatg ggagaggcat
     2881 ggcagaggac tacatggtcc ttgacttgag caccacctcc agcctccagt ccagcagcag
     2941 tatccattcc tccagagaat ccgacgcagg cagcgatgag gggattcttc tcgatgacat
     3001 tgacggggcg agtgacagtg gggagtcggc acacaaggcc gaggcccctg ccctccctgg
     3061 cagcctaggg gctgaagttt caggatctct tatgttcagc agcttgtctg ggagcaatgg
     3121 tgggatcatg tgcaacattt gccacaaaat gtacagcaac aaggggaccc tgagagtgca
     3181 ctacaaaact gtgcatttga gagaaatgca caagtgcaaa gtcccaggtt gcaatatgat
     3241 gttttcctct gtacgaagcc gaaatcggca cagtcagaac cctaatctcc acaaaaacat
     3301 tcccttcact tcagtagatt agtctcagaa tggacactac aaatgccagc tctcaccaga
     3361 tggcctacgt gtttgaactg ccatagtcag tgtgcgctta tgtacttggg gtgtgtgtgt
     3421 gtgtgtgtgt gtgtgtgcat ttatgtatgc tctgtggcta catatacaca cacgtatttc
     3481 cttgagataa acaagataaa cactaggtgc ttttgaattt ttttcacttc cctttatagt
     3541 tttgggaaag gagtgggatc tttgatttca gggtgaaaac agagtacccc tttaaacaca
     3601 cacacacaca catgcacata cacacacaca cacacacaca cacacagtgt gcaactagcc
     3661 ccagttttga cagaataatt cttggtcttc cccaaagaga caatttgttg tacccatgac
     3721 tgttgcctgc aaaaataaaa gggaaaaaaa agaaaaaaga aacaaaaagg aacttcttat
     3781 agttgtcttt tgtgaacttt aaggttttga aagaatcttc aattaaagca tggcagattc
     3841 acctgtaaat atttagcctt gaggggccat tgatgtaaaa caattgtatt gatggttgtt
     3901 taactttttt gtttaatttt tacagttaca tccagctgtt agatatgcag gaaaagatag
     3961 tttgctggct agctctattc atttatgtta gcattaatgc acatttttaa aaaaagaaaa
     4021 aaacatggtc ttgtttttac tacctgttag atatagtgct aaagaggtgc tggcatgctt
     4081 tacagcacag atctgatttt ttaaaatgtc ctgtactagt acataaatcc cgtcatgcac
     4141 tttttttcat acactacaag gggatgtgta ataaccatgc ttctttttta tccttaaact
     4201 attgccatac ttcagtaagt gtctttttta aaaaaaattc acttgtataa aaatggtctg
     4261 gtcagtatag ggcacaaatg ccaaacaaag tattagtgtt aacacaaaac tgccaacttt
     4321 gcacaagttt ccagaaaaga aaatacaatt agtcactcaa cataaccaca ggccaatttg
     4381 ttggccacca gaaaaccgct ttttaaaaaa acacttggtg attctttcaa gtgccgaatg
     4441 ttattagaat caacattgca tccttcttgc ttatacgtta agttacataa aaggaaaaca
     4501 aaattatgtg gtgtgaaaca gccaaggcat ttattcttca gaggcaggat aatatttcag
     4561 gatacaaaag cccaaattac ccgctacgga acaaagatga atacagtaaa agagtcgaac
     4621 actcatttcc aaggccctaa cccttatcct ttaaaaagaa atcctctgaa ctgggtcggt
     4681 ctgttgtatg gctgtctaat ttgttgcaaa ttctgcagtc gtactataat tcaggcctgt
     4741 ttggtagaca aaatcaaaag gcatttaaca gcagcagtac ttggaagctt tgaaaacatg
     4801 tgctaaactt gaatggagca acactccttt ctgaaaagcc agaagaaggg ggttttaaag
     4861 caggatctct caatgttcag tgttttgtgt ttccccacac gatttgtcag agagaaatga
     4921 aagcaagcct gaaaccaagc aattgagagt gagaaagagg agagagacta ttctccaaac
     4981 cttttttgtt ttgttttgtt tccgatgttt acacatgttg cctgagctgg ttaaccacat
     5041 cggcagcctc agctaccaca acatactgac taaatgatga tatttactca agttcagtct
     5101 gcagccaaaa ccattagggt gtgcattgca gactgttttg ttgtcttttt tttttctttt
     5161 ttcctttttt tttaagtgga ggggaaaaga agagataaac aaggaatatt ttgtcaaaca
     5221 gtacacaata atttaaagag aatgtatttc ttttttgcat ttaatggcct cagacatttc
     5281 tttcatcagt ctaaaagtta gaaatatccc tttattttaa cttttatgtc gtttccattt
     5341 ttcatgtttt tgtaattatt ttttctatgt tcacatcagc attcacttcc gttaaatttg
     5401 ccaaaggaaa ctgttaatat gtttctgttg ttattattcc tgtaggattt attgtacctc
     5461 acagtattta ttgtttccca aaataagcat cattagttgg ggattcagta tttttgttgt
     5521 gaaaatttca gaaacaatag attcttaaag ataagctagc tatgtctagg agctttatct
     5581 tttcacctcc ttcagaggat gctatggggt ccattttaat tcttcatttg ttctacgggg
     5641 aggaaagacc aaaagtattt gcagtacaaa agaaactata tcaaacacta tgttaaatga
     5701 caagtgttta tggtaaaaag ctgaggaatt agtaataatc gtttttgttt tcttgtactt
     5761 tgaattcccc aaagtcttaa ttgctttatt ttggtgttgt gttaaattga acagacagat
     5821 gttttccttt gttttatacc atataagact ctgtacagta tgtttgtgaa agattgaact
     5881 agaataatga aagtttgttt gcaaacattt tggtcctctc agcgttctct gtgttgtgct
     5941 gttgccccat taccaaatgg ttagattcaa aaagggtggg aaaagatttt taatatccca
     6001 acaaaacatt atagaaactc tggcttttgc agtgtgcata gactacatgt agttttatga
     6061 aaataaatac acatttttat ttcagcaact ttgaaaagtt acactcagtt gagttactag
     6121 aactcatctt gtacaacagc aatgtttagt ctgtttaatt taatgtcaaa taaaaggtca
     6181 gatagccctc gtgagtgaat tacatagctg tcggcagtca catcagcaaa tgcaccaggc
     6241 ttagaacaaa tgtttgttac ttgctgcaaa ccaagctaat gtgtatagcc agttagaaaa
     6301 agtccagaag taatgagatt ctagaagtag agtttccctt gcttggaaac atgaatgtgt
     6361 tcacctgtgt tttgtcagag aagtggcaat aagtcctgga cagctgacac tttttaagta
     6421 tctcccctat ttgctactac ttttgtgcct caagtgccca gtcgttacgg tggccttcta
     6481 aatgagtaaa taacattttc caatataaag cctatttgct taaaagggac aggggagtgg
     6541 atggatgtag tacatgcaat ggaaaatcat aaaatgtaca attcttctgt ttccaaagta
     6601 ttgctcgttc ttgagtgtgt gcctgagtgt cttgttttga cttaactaga attctagtta
     6661 agatggtgat cccatggctt atttgcaaac aggaaggata aagagatcag cgttttagtc
     6721 atggagctca tctttgccca ttcctactca tttgcttttt cataggagtt ttttgttgtt
     6781 aaacagtttc tctcaagcca catgcattct atgctggctg aaaattaata gtgatgtaga
     6841 tgttcatccg acaagctttt ccccatgtga ttggttttag ccagagttta tcacaggtac
     6901 aaaattaggc ggtatgactg tgcatgttct ctagctgatg ttgaaacttg tactgtcttg
     6961 atcatttaaa actatgtttt tgtaaatatc aggtttagtc cgctttcggg aatatctgca
     7021 tgctatggaa agagagaaaa aaaggataga taatgaggaa tttggtttaa aagtgtgata
     7081 aaaatccttg gcattctttg ttctgatatt aattgtattt aaataagtca acccaatgta
     7141 actcattcca gatcttagtc cagctgccct gttcagcctg atgcctttta aaggtttaaa
     7201 ggtgttaatg ttttcctttt caacatggca aacatttttt aaaacctttt tggcacaatg
     7261 gtgccactgt cctcatagtg ttatttcttt ggtcatgaat tttcaggccc ttccagtaag
     7321 aggagaaagg cacttaactg gttaacagcc ccattattat acattgctct aaggaaaaaa
     7381 aaaaaaaaaa aactttgaat atatcaagaa tgggctattc cagaggcttc tcctcaggag
     7441 aaactatgca ctaggttccc accaaataca atgtgacctt tttttcccct ttctctgtac
     7501 acaccccaga tatgtgccaa gcaaatgaga atgagggccg ttgacaatta aagcataaag
     7561 aagctagtca ccagattgag atggacatgc tgctaactgc tgacagcttg acctgagcag
     7621 tcctaacttg tatctggtct gttagcattg ggttagattg actaacagag taaataaaat
     7681 tcacctgtca taagacacgc tacatctgct atcatcaaga aacaaatcat ccagaagaaa
     7741 ctttttttca tcctgtggtc acaccctttt cttaagagct tcttttttaa aattatgaca
     7801 aacgaatttc attctttaaa atcacttatc ttctcccaaa cttgaagtat ttaaattagg
     7861 tggttttgct tttccctctc attgtttatt ttttggggat ggatctaaat gagtaagatg
     7921 agcaataaaa gataccagaa agactggagg gatcacagtg ttttcatcag aactaagtag
     7981 agggtcggtt cctgccctgg ttttggggta gctagaaaag gaaatcatga atgcactgga
     8041 aatgttggct cgagagaaaa gactggcata gctctgcctc gcagtgggtg agagcgtatt
     8101 gatcgtaggg acctgcagag ttcctatgac caggtgagtc ggctctgagg aaggggttag
     8161 gaaagcaagt acagctgcat gaaatagctg aagttccttt ggggtcagga agagccgaca
     8221 attgtggggt tttggttgct tttgctttgt ttttgagtgg gaagtgttgc tggaatcccc
     8281 tgagaaccga aagatgccag gggtcagcaa ggctatagaa aaggccagtg gtaacacctc
     8341 gttttcatcc taacagagga gtaggtgcga atggcaccag catggatcgt tcctttcttg
     8401 atcattactc atgcttgcca ctgtagcaac acaacaaagc catgattgta attaatacta
     8461 ggctaaagac cttgatcaaa atgggagctc atttactgtt ctatgaggta tgtaagtaac
     8521 ttctcttcac attttccctc agagataaac cacacgtggt agtcttgttt gcgttagtac
     8581 taaggtcatg tttgatctgt cccagacgag tcgcatattt cctgtagctg gagtgttgct
     8641 caccataaat ggtcattttc aaaaagtatt gtgtaattcc agctgtacaa ctgaccagtg
     8701 agttatctgt catcactgtt gcccacatac ccgtccttcc ttgtgtagtt tcatcatcct
     8761 catccttccc acatcttgcg caaaatgatt ttctgtgctc attcggcaat aactatgtta
     8821 ttcggcatac tgtctttttg gcatttgttg aaaaatccaa atgcttttaa tccaaaagat
     8881 taatccaaat cttttttaat ccaaatgatt ttaatccaaa tccaaataac aggaggttaa
     8941 aaaaaaaaaa aaaactaagc atttttacat gtacactgaa ttggatcagc attattgttc
     9001 attataatgc tatatatatt tttggagcaa catactatag ttgtcataaa actatcttta
     9061 ttcttctcct ctaaagtgct gttgtaaatt catttgacct ttactgcagg aaaaaaaaaa
     9121 atctttttta tataggatat gaagaccaag gctcagtctg taacaaatag tggaccatta
     9181 caaaagagga aagaaaaaag ccagggctga gcagcaagaa gcttcagttc aatttcacct
     9241 cgtatctttc taataacata cttgtcactt tattaccttc cagtaatgtg aacgctgctg
     9301 acaagactgt cacactaaca gcagacaaca ccctctctgc ttcagcccag ctctcctggc
     9361 tacttattgc cctggccctg aagggacaca aactaatagt gtgctttcca gttcagcact
     9421 tggccatcaa atataaaagg atgcgtaatt gcctgtttat ccccttgtga aggagaaatt
     9481 gactacaagg gctaggcttt cccatcgagt tccctgatgt acacacacat ccacaatcag
     9541 tcagtctctc tctctctctc tctctctctc tctctctctc tctctctctc ccctctctct
     9601 ctccttccct cttattctcc cctcctaaca tacaagcaca tacacacacc tactgtatct
     9661 gtgggaagcc caaggctgcc tctcgatgcc ctctccagct attaagtgga cagtaacaag
     9721 atgacttctt aaataaaggg tcagtagaga gatgctgtct gtgacggcat cctttgcttc
     9781 tttgaaaact taagtgttaa caattccatc ttgagagtaa tgggtgtggc cctataatta
     9841 gagcaaattt tcctgcaagt cagtggcata agtaagatta tattgccccc taatctgtag
     9901 ggaaaagatt aaaatatttt tcttcccctt caaaaagtgc acatgttgta aactggttac
     9961 gcacagtggt catttttttt tcttatcctt ggcatcaaac catggacgta ggtgtacaaa
    10021 tgcatctttc aatgacccct ccaaatccat agtgtaacat gatagattaa cttttgtcaa
    10081 actggactga caatctaaaa aagatggcgt caggcttttg actttaatcg ttaaaacaag
    10141 gaccacccta tgagtaaaag gtaaattctc cactttgaag aactttggta agtacaagta
    10201 cagggagctt tagaaaatcg agtgatgggg accaaaggta caaaggaaga atacatgaaa
    10261 atgttatatt ccaaaatgcc ctgagcccaa agattggctg ctatttttgt atattgaaat
    10321 actcaaatga tggttggaaa gtgactagtt agtgactaaa tgaagtgcaa gatctatcaa
    10381 gcgtcttctt tctgggacta ggggatgcca tgcttccttc caaccttccc gcagcgcatc
    10441 tcagagaaga tagttggccc cattacaaac agtcacattt cagtaagata ttagtcagcc
    10501 aggtaagaca cgtgcaggta tcggtatcat cttccatatg aactttagca aggagagaac
    10561 agcagcagat ttagggatag acaatgtaac aggtctatgt tgacaaatct gtgctaaata
    10621 atttcatgaa ccagtgtggg gactgggaag aaaagcactt tgagcaagga ctcttgggtt
    10681 cgagcaaagg caattagttg acatgatact tcttaagttc ctcagtatgt atatgttaca
    10741 atcgtttatc agacatctta atagaatcct aattgaaaaa ccagttgcca aaattgcaca
    10801 agttctgctc gctgatacta gctttctccc cttaactcta aaataccagg gatgcttaag
    10861 gacgtttgta atagtttttt taatgctttg tttcactttt ttaaaaaaga atctttgaag
    10921 ggaaggaaga gcagaaagaa gtattttaag aaaaatggag agaagtagat agtattgaaa
    10981 gtatatttct tgaagagaga gtatctaagt gctataccaa gattttatag ggcttcctgt
    11041 tgccaaatgt gatgttgaga taatgcacag ctaagatggc aaatccatca attattaact
    11101 ggctctgccc acttctgtca tggaatgcaa ggattgagag gtgactctgg ggagaccctg
    11161 ggtgtgtgag agagctccat tcatctggcc ctggatatgt ttttcaaaag agagggagaa
    11221 agcgccagtc cctgcaaggt gaactgacct ggcactgttt cagtgggagc ctcactgcct
    11281 gccttttcca tgctaggaga caaagcatcc tctaccccat ctgtgaatcg gtgctgtggc
    11341 cactgcgaga agcatgattc atgaggtatg atgctcttga gctcccagac aatgtgctga
    11401 gttaataggt tcacttgaga tgtatacacc aaggctgttt ctttttttaa atctagtccc
    11461 caatttggag tatttttgca tgtttttgta cagagtaatc cattcctctc attgtgtatc
    11521 ttaatctcct ctgacttttc cattgtcttt ctcaatccca ccctttgctc ttcggatctc
    11581 accaaccccc cttaaaaaat aaatcatgtt tgagcaagaa ggtagaacac gccctccctc
    11641 atcttggttt taattgcttt ggaaacgtgt tctaccctgt ccagggtttg cataacgtga
    11701 attaagtgaa tgagatgttc tagtattata tcttaacctg ataagactat ctaagatttc
    11761 tagtatatgg tgcatttgct ttcctgtgca aactttggtt cagctgccct gcagagaatc
    11821 tcaccatttt cctgccagtg ccagtataaa gaatgcagga gagctaaacc tgggtacatg
    11881 aaggtcagag gggtgaggac ggtcgagaaa tggggagaag acttgggctt gagacgacct
    11941 gggcttttca tgtgtagctc actcagcagt atgaggatga ctgacacacc agtgggtggt
    12001 ttccaagtga ggcaaatgcc catttcccct ctcccctcac accttgcctg gcttcttcca
    12061 tgaagtcctt gctgcttttc tgcctcccca aaggtgaggg gaaggggctg gttggggatc
    12121 tgggaaagcc agttctctgt tctctcctgc tggtgatgga ctaggccttt tagaactagc
    12181 aagatccctc acacagctgg gagaacacac acctttctta ctccagaccc attggtgtgt
    12241 ctccagtaac aaaattattg gactcagcct ccatatttga cagcaaaagt ggccagaggg
    12301 agttgaaata tcttgaagaa aaggaatttt cactaagata tgtcctctcc ctctcccaga
    12361 gtttagctgt ttattccttt tttttgttta tattgttctc atctgcataa aaccagtctc
    12421 ttgcaataag cctgccgcag aatcaaagtc tgtacttcaa aaggtaactg caccaaggga
    12481 tgggacagtg tgcatcaccc tgatctaatc attgtgacgt tggtagcttc ctaaatactg
    12541 tatgtacctt gaacaagggt tttatttttg ttttgttctg ttttgctttt tgtttttatt
    12601 ggtaggctaa ggtaattaaa ttttttaatt tgctgttact ttggttgtat tttctgtact
    12661 ataactgcct acagtatgtc ttttgcataa aatgcataag ggtttgggga tgtaaatgga
    12721 attttattca tattttgtcc aaatacctct tgtaatttgt atcaaaattc ttgtacaatt
    12781 tttatattaa agatttatca gtcactga
//
