LOCUS       NM_000386               2307 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens bleomycin hydrolase (BLMH), mRNA.
ACCESSION   NM_000386
VERSION     NM_000386.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   3  (bases 1 to 2307)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 2307)
  AUTHORS   Riise,R., Odqvist,L., Mattsson,J., Monkley,S., Abdillahi,S.M.,
            Tyrchan,C., Muthas,D. and Yrlid,L.F.
  TITLE     Bleomycin hydrolase regulates the release of chemokines important
            for inflammation and wound healing by keratinocytes
  JOURNAL   Sci Rep 9 (1), 20407 (2019)
   PUBMED   31892708
  REMARK    GeneRIF: Bleomycin hydrolase regulates the release of chemokines
            important for inflammation and wound healing by keratinocytes.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2307)
  AUTHORS   Wu,H., Li,L., Chen,K.M., Homolka,D., Gos,P., Fleury-Olela,F.,
            McCarthy,A.A. and Pillai,R.S.
  TITLE     Decapping Enzyme NUDT12 Partners with BLMH for Cytoplasmic
            Surveillance of NAD-Capped RNAs
  JOURNAL   Cell Rep 29 (13), 4422-4434 (2019)
   PUBMED   31875550
  REMARK    GeneRIF: Decapping Enzyme NUDT12 Partners with BLMH for Cytoplasmic
            Surveillance of NAD-Capped RNAs.
REFERENCE   6  (bases 1 to 2307)
  AUTHORS   Koldamova,R.P., Lefterov,I.M., Gadjeva,V.G. and Lazo,J.S.
  TITLE     Essential binding and functional domains of human bleomycin
            hydrolase
  JOURNAL   Biochemistry 37 (8), 2282-2290 (1998)
   PUBMED   9485374
REFERENCE   7  (bases 1 to 2307)
  AUTHORS   Ferrando,A.A., Pendas,A.M., Llano,E., Velasco,G., Lidereau,R. and
            Lopez-Otin,C.
  TITLE     Gene characterization, promoter analysis, and chromosomal
            localization of human bleomycin hydrolase
  JOURNAL   J Biol Chem 272 (52), 33298-33304 (1997)
   PUBMED   9407121
REFERENCE   8  (bases 1 to 2307)
  AUTHORS   Montoya,S.E., Ferrell,R.E. and Lazo,J.S.
  TITLE     Genomic structure and genetic mapping of the human neutral cysteine
            protease bleomycin hydrolase
  JOURNAL   Cancer Res 57 (19), 4191-4195 (1997)
   PUBMED   9331073
REFERENCE   9  (bases 1 to 2307)
  AUTHORS   Bromme,D., Rossi,A.B., Smeekens,S.P., Anderson,D.C. and Payan,D.G.
  TITLE     Human bleomycin hydrolase: molecular cloning, sequencing,
            functional expression, and enzymatic characterization
  JOURNAL   Biochemistry 35 (21), 6706-6714 (1996)
   PUBMED   8639621
REFERENCE   10 (bases 1 to 2307)
  AUTHORS   Ferrando,A.A., Velasco,G., Campo,E. and Lopez-Otin,C.
  TITLE     Cloning and expression analysis of human bleomycin hydrolase, a
            cysteine proteinase involved in chemotherapy resistance
  JOURNAL   Cancer Res 56 (8), 1746-1750 (1996)
   PUBMED   8620487
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC346179.1 and BC003616.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_000386.3.
            
            Summary: Bleomycin hydrolase (BMH) is a cytoplasmic cysteine
            peptidase that is highly conserved through evolution; however, the
            only known activity of the enzyme is metabolic inactivation of the
            glycopeptide bleomycin (BLM), an essential component of combination
            chemotherapy regimens for cancer. The protein contains the
            signature active site residues of the cysteine protease papain
            superfamily. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.224542.1,
                                           SRR1163658.220035.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261714.11/ ENSP00000261714.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                DC346179.1         223-248
            27-2307             BC003616.2         1-2281
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..2307
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /note="bleomycin hydrolase"
                     /db_xref="GeneID:642"
                     /db_xref="HGNC:HGNC:1059"
                     /db_xref="MIM:602403"
     exon            1..138
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..1493
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /EC_number="3.4.22.40"
                     /note="BLM hydrolase"
                     /codon_start=1
                     /product="bleomycin hydrolase"
                     /protein_id="NP_000377.1"
                     /db_xref="CCDS:CCDS32604.1"
                     /db_xref="GeneID:642"
                     /db_xref="HGNC:HGNC:1059"
                     /db_xref="MIM:602403"
                     /translation="MSSSGLNSEKVAALIQKLNSDPQFVLAQNVGTTHDLLDICLKRA
                     TVQRAQHVFQHAVPQEGKPITNQKSSGRCWIFSCLNVMRLPFMKKLNIEEFEFSQSYL
                     FFWDKVERCYFFLSAFVDTAQRKEPEDGRLVQFLLMNPANDGGQWDMLVNIVEKYGVI
                     PKKCFPESYTTEATRRMNDILNHKMREFCIRLRNLVHSGATKGEISATQDVMMEEIFR
                     VVCICLGNPPETFTWEYRDKDKNYQKIGPITPLEFYREHVKPLFNMEDKICLVNDPRP
                     QHKYNKLYTVEYLSNMVGGRKTLYNNQPIDFLKKMVAASIKDGEAVWFGCDVGKHFNS
                     KLGLSDMNLYDHELVFGVSLKNMNKAERLTFGESLMTHAMTFTAVSEKDDQDGAFTKW
                     RVENSWGEDHGHKGYLCMTDEWFSEYVYEVVVDRKHVPEEVLAVLEQEPIILPAWDPM
                     GALAE"
     exon            139..336
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            337..446
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            447..588
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            589..677
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            678..770
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            771..926
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1085
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1153
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1271
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1341
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..2307
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2284..2289
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /note="hexamer: AATAAA"
     polyA_site      2307
                     /gene="BLMH"
                     /gene_synonym="BH; BMH"
                     /note="major polyA site"
ORIGIN      
        1 ctttttccgg cgctccgggt gcgagagaca ggtcgggccc cctaggcagc gagccgcagc
       61 gcaatcccgg cgctcgccca aggaccctgg aagctaccgt taccccgccg ggcagcgtgg
      121 gcgccatgag cagctcggga ctgaattcgg agaaggtagc tgctctgata cagaaactga
      181 attccgaccc ccagttcgta cttgcccaga atgtcgggac cacccacgac ctgctggaca
      241 tctgtctgaa gcgggccacg gtgcagcgcg cgcagcatgt gttccagcac gccgtgcccc
      301 aggagggcaa gccaatcacc aaccagaaga gctcagggcg atgctggatc ttttcttgtc
      361 tgaatgttat gaggcttcca ttcatgaaaa agttaaatat tgaagaattt gagtttagcc
      421 aatcttacct gtttttttgg gacaaggttg aacgctgtta tttcttcttg agtgcttttg
      481 tggacacagc ccagagaaag gagcctgagg atgggaggct ggtgcagttt ttgcttatga
      541 accctgcaaa tgatggtggc caatgggata tgcttgttaa tattgttgaa aaatatggtg
      601 ttatccctaa gaaatgcttc cctgaatctt atacaacaga ggcaaccaga aggatgaatg
      661 atattctgaa tcacaagatg agagaattct gtatacgact gcggaacctg gtacacagtg
      721 gagcaaccaa aggagaaatc tcggccacac aggacgtcat gatggaggag atattccgag
      781 tggtgtgcat ctgtttgggt aatccaccag agacattcac ctgggaatat cgagacaaag
      841 ataaaaatta tcagaaaatt ggccccataa cacccttgga gttttacagg gaacatgtca
      901 agccactctt caatatggaa gataagattt gtttagtgaa tgaccctagg ccccagcaca
      961 agtacaacaa actttacaca gtggaatact taagcaatat ggttggaggg agaaaaactc
     1021 tatacaacaa ccagcccatt gacttcctga aaaagatggt tgctgcctcc atcaaagatg
     1081 gagaggctgt gtggtttggc tgtgatgttg gaaaacactt caatagcaag ctgggcctca
     1141 gtgacatgaa tctctatgac catgagttag tgtttggtgt ctccttgaag aacatgaata
     1201 aagcggagag gctgactttt ggtgagtcac ttatgaccca cgccatgacc ttcactgctg
     1261 tctcagagaa ggatgatcag gatggtgctt tcacaaaatg gagagtggag aattcatggg
     1321 gtgaagacca tggccacaaa ggttacctgt gcatgacaga tgagtggttc tctgagtatg
     1381 tctacgaagt ggtggtggac aggaagcatg tccctgaaga ggtgctagct gtgttagagc
     1441 aggaacccat tatcctgcca gcatgggacc ccatgggagc tttggctgag tgatactgcc
     1501 ctccagctct ttcctccttc catggaacct gacgtagctg caaaggacag atccagggac
     1561 tgaagccaaa gttatgcaag ggactgtgtg ttgccacagg acacagtcag atttccagtc
     1621 tccaccagga acctcttcag aaagtgtgct ttatgctgaa acagaatact gttaaaggaa
     1681 aaaaaagagg ggggaagatc aggtcatact atctactctc ctcatctcta acagctcagg
     1741 atctcttagc attttaatta gatgtaattg tttgtcttta actgtcaaaa agtttggttc
     1801 tgtgtctgtg ttttaataag acgagaggac gagcgattga ggtgtatgga gagaaaacag
     1861 acctaatgct ccttgttcct agagtagagt ggagggaggg tggcctaaga gttgagctct
     1921 cggaactgca tgctgctgga cagtatcact gtctttccta gatggcagtc actgaattcc
     1981 attttttcaa ggtaatttct tgtgcctcta atagcccaag aatgggaggt tgatcagatc
     2041 tgacatgatt ccttcctgtt ctgaactgtg gggtgtgcac atctctgctt gagtcaggtt
     2101 tgagtagagg cttagagaca gttgggtgag aacaaccaaa atcttatcat ggtctcagtc
     2161 ataatcatta gggggaactc tagccaaatg gtttaacttc tgcctgtgga actggggatt
     2221 gggtgggcag gaaaaggtga tatccattct ttctgataac tagatggtgc tgagaagctt
     2281 ttgaataaaa actttgctaa atgagaa
//
