LOCUS       NM_001287246            5350 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens BLM RecQ like helicase (BLM), transcript variant 2,
            mRNA.
ACCESSION   NM_001287246
VERSION     NM_001287246.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Silva,J.P.L., Donaires,F.S., Gutierrez-Rodrigues,F., Martins,D.J.,
            Carvalho,V.S., Santana,B.A., Cunha,R.L.G., Kajigaya,S.,
            Menck,C.F.M., Young,N.S., Kjeldsen,E. and Calado,R.T.
  TITLE     RecQ helicase expression in patients with telomeropathies
  JOURNAL   Mol Biol Rep 51 (1), 754 (2024)
   PUBMED   38874681
  REMARK    GeneRIF: RecQ helicase expression in patients with telomeropathies.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Tsukada,K., Jones,S.E., Bannister,J., Durin,M.A., Vendrell,I.,
            Fawkes,M., Fischer,R., Kessler,B.M., Chapman,J.R. and
            Blackford,A.N.
  TITLE     BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint
            DNA molecule resolution
  JOURNAL   Mol Cell 84 (4), 640-658 (2024)
   PUBMED   38266639
  REMARK    GeneRIF: BLM and BRCA1-BARD1 coordinate complementary mechanisms of
            joint DNA molecule resolution.
REFERENCE   3  (bases 1 to 5350)
  AUTHORS   Liu,S., Atkinson,E., Paulucci-Holthauzen,A. and Wang,B.
  TITLE     A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism
            controlling BLM ubiquitination and G-quadruplex resolution
  JOURNAL   Nat Commun 14 (1), 6111 (2023)
   PUBMED   37777511
  REMARK    GeneRIF: A CK2 and SUMO-dependent, PML NB-involved regulatory
            mechanism controlling BLM ubiquitination and G-quadruplex
            resolution.
            Erratum:[Nat Commun. 2024 Feb 5;15(1):1077. doi:
            10.1038/s41467-024-45551-1.. PMID: 38316763]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5350)
  AUTHORS   Danino,Y.M., Molitor,L., Rosenbaum-Cohen,T., Kaiser,S., Cohen,Y.,
            Porat,Z., Marmor-Kollet,H., Katina,C., Savidor,A., Rotkopf,R.,
            Ben-Isaac,E., Golani,O., Levin,Y., Monchaud,D., Hickson,I.D. and
            Hornstein,E.
  TITLE     BLM helicase protein negatively regulates stress granule formation
            through unwinding RNA G-quadruplex structures
  JOURNAL   Nucleic Acids Res 51 (17), 9369-9384 (2023)
   PUBMED   37503837
  REMARK    GeneRIF: BLM helicase protein negatively regulates stress granule
            formation through unwinding RNA G-quadruplex structures.
REFERENCE   5  (bases 1 to 5350)
  AUTHORS   Foucault,F., Vaury,C., Barakat,A., Thibout,D., Planchon,P.,
            Jaulin,C., Praz,F. and Amor-Gueret,M.
  TITLE     Characterization of a new BLM mutation associated with a
            topoisomerase II alpha defect in a patient with Bloom's syndrome
  JOURNAL   Hum Mol Genet 6 (9), 1427-1434 (1997)
   PUBMED   9285778
REFERENCE   6  (bases 1 to 5350)
  AUTHORS   Ellis,N.A., Groden,J., Ye,T.Z., Straughen,J., Lennon,D.J.,
            Ciocci,S., Proytcheva,M. and German,J.
  TITLE     The Bloom's syndrome gene product is homologous to RecQ helicases
  JOURNAL   Cell 83 (4), 655-666 (1995)
   PUBMED   7585968
REFERENCE   7  (bases 1 to 5350)
  AUTHORS   German,J., Roe,A.M., Leppert,M.F. and Ellis,N.A.
  TITLE     Bloom syndrome: an analysis of consanguineous families assigns the
            locus mutated to chromosome band 15q26.1
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6669-6673 (1994)
   PUBMED   8022833
REFERENCE   8  (bases 1 to 5350)
  AUTHORS   Langer,K., Cunniff,C.M. and Kucine,N.
  TITLE     Bloom Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301572
REFERENCE   9  (bases 1 to 5350)
  AUTHORS   Turner,J.T., Brzezinski,J. and Dome,J.S.
  TITLE     Wilms Tumor Predisposition
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301471
REFERENCE   10 (bases 1 to 5350)
  AUTHORS   McDaniel,L.D. and Schultz,R.A.
  TITLE     Elevated sister chromatid exchange phenotype of Bloom syndrome
            cells is complemented by human chromosome 15
  JOURNAL   Proc Natl Acad Sci U S A 89 (17), 7968-7972 (1992)
   PUBMED   1518822
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD707743.1, BP363946.1,
            BC143288.1, BC115032.1, DB520927.1 and AC124248.6.
            
            On May 31, 2019 this sequence version replaced NM_001287246.1.
            
            Summary: The Bloom syndrome is an autosomal recessive disorder
            characterized by growth deficiency, microcephaly and
            immunodeficiency among others. It is caused by homozygous or
            compound heterozygous mutation in the gene encoding DNA helicase
            RecQ protein on chromosome 15q26. This Bloom-associated helicase
            unwinds a variety of DNA substrates including Holliday junction,
            and is involved in several pathways contributing to the maintenance
            of genome stability. Identification of pathogenic Bloom variants is
            required for heterozygote testing in at-risk families. [provided by
            RefSeq, May 2020].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC143288.1, SRR14038191.1613662.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-20                CD707743.1         41-60
            21-58               BP363946.1         33-70
            59-1669             BC143288.1         1-1611
            1670-4517           BC115032.1         1527-4374
            4518-4638           DB520927.1         335-455
            4639-5350           AC124248.6         57935-58646
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..5350
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="BLM RecQ like helicase"
                     /db_xref="GeneID:641"
                     /db_xref="HGNC:HGNC:1058"
                     /db_xref="MIM:604610"
     exon            1..95
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            96..205
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            206..307
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..4463
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /EC_number="5.6.2.4"
                     /note="isoform 1 is encoded by transcript variant 2; bloom
                     syndrome protein; recQ protein-like 3; Bloom syndrome RecQ
                     like helicase; DNA helicase, RecQ-like type 2; Bloom
                     syndrome, RecQ helicase-like; recQ-like DNA helicase BLM;
                     DNA 3'-5' helicase BLM"
                     /codon_start=1
                     /product="recQ-like DNA helicase BLM isoform 1"
                     /protein_id="NP_001274175.1"
                     /db_xref="CCDS:CCDS10363.1"
                     /db_xref="GeneID:641"
                     /db_xref="HGNC:HGNC:1058"
                     /db_xref="MIM:604610"
                     /translation="MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSD
                     NNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRG
                     GSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDF
                     DTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQI
                     DLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLE
                     EAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDV
                     LSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLK
                     LLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSM
                     KELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAET
                     PRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDD
                     WEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQ
                     NYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLG
                     EDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGD
                     KTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAH
                     CVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMS
                     FNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLA
                     ALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEG
                     YYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYC
                     ENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFV
                     QEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLF
                     KKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKAL
                     VAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQ
                     IDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIP
                     VSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIG
                     SSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS"
     exon            308..1008
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1168
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1296
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1429
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..2091
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2283
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2402
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2516
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2615
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2616..2764
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2765..2871
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..3032
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3033..3228
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3229..3419
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3567
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3767
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3768..3960
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3961..4083
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            4084..4285
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            4286..5350
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4526..4531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="hexamer: TATAAA"
     polyA_site      4552
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="major polyA site"
     regulatory      4621..4626
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="hexamer: AATAAA"
     polyA_site      4646
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
     regulatory      5330..5335
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="hexamer: AATAAA"
     polyA_site      5350
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
ORIGIN      
        1 ccggcgggaa gtgagccagg gcttggcgcg gcggccgtgg ttgcggcgcg ggaagtttgg
       61 atcctggttc cgtccgctag gagtctgcgt gcgagacagg gtcttgctca gctatccagg
      121 ctggagtgca gtagaatgat cgttgctcac tgcagcctct aactcctggg ctcagatgat
      181 ccttctgcct cagcctcccg agaaggatta tggctgctgt tcctcaaaat aatctacagg
      241 agcaactaga acgtcactca gccagaacac ttaataataa attaagtctt tcaaaaccaa
      301 aattttcagg tttcactttt aaaaagaaaa catcttcaga taacaatgta tctgtaacta
      361 atgtgtcagt agcaaaaaca cctgtattaa gaaataaaga tgttaatgtt accgaagact
      421 tttccttcag tgaacctcta cccaacacca caaatcagca aagggtcaag gacttcttta
      481 aaaatgctcc agcaggacag gaaacacaga gaggtggatc aaaatcatta ttgccagatt
      541 tcttgcagac tccgaaggaa gttgtatgca ctacccaaaa cacaccaact gtaaagaaat
      601 cccgggatac tgctctcaag aaattagaat ttagttcttc accagattct ttaagtacca
      661 tcaatgattg ggatgatatg gatgactttg atacttctga gacttcaaaa tcatttgtta
      721 caccacccca aagtcacttt gtaagagtaa gcactgctca gaaatcaaaa aagggtaaga
      781 gaaacttttt taaagcacag ctttatacaa caaacacagt aaagactgat ttgcctccac
      841 cctcctctga aagcgagcaa atagatttga ctgaggaaca gaaggatgac tcagaatggt
      901 taagcagcga tgtgatttgc atcgatgatg gccccattgc tgaagtgcat ataaatgaag
      961 atgctcagga aagtgactct ctgaaaactc atttggaaga tgaaagagat aatagcgaaa
     1021 agaagaagaa tttggaagaa gctgaattac attcaactga gaaagttcca tgtattgaat
     1081 ttgatgatga tgattatgat acggattttg ttccaccttc tccagaagaa attatttctg
     1141 cttcttcttc ctcttcaaaa tgccttagta cgttaaagga ccttgacacc tctgacagaa
     1201 aagaggatgt tcttagcaca tcaaaagatc ttttgtcaaa acctgagaaa atgagtatgc
     1261 aggagctgaa tccagaaacc agcacagact gtgacgctag acagataagt ttacagcagc
     1321 agcttattca tgtgatggag cacatctgta aattaattga tactattcct gatgataaac
     1381 tgaaactttt ggattgtggg aacgaactgc ttcagcagcg gaacataaga aggaaacttc
     1441 taacggaagt agattttaat aaaagtgatg ccagtcttct tggctcattg tggagataca
     1501 ggcctgattc acttgatggc cctatggagg gtgattcctg ccctacaggg aattctatga
     1561 aggagttaaa tttttcacac cttccctcaa attctgtttc tcctggggac tgtttactga
     1621 ctaccaccct aggaaagaca ggattctctg ccaccaggaa gaatcttttt gaaaggcctt
     1681 tattcaatac ccatttacag aagtcctttg taagtagcaa ctgggctgaa acaccaagac
     1741 taggaaaaaa aaatgaaagc tcttatttcc caggaaatgt tctcacaagc actgctgtga
     1801 aagatcagaa taaacatact gcttcaataa atgacttaga aagagaaacc caaccttcct
     1861 atgatattga taattttgac atagatgact ttgatgatga tgatgactgg gaagacataa
     1921 tgcataattt agcagccagc aaatcttcca cagctgccta tcaacccatc aaggaaggtc
     1981 ggccaattaa atcagtatca gaaagacttt cctcagccaa gacagactgt cttccagtgt
     2041 catctactgc tcaaaatata aacttctcag agtcaattca gaattatact gacaagtcag
     2101 cacaaaattt agcatccaga aatctgaaac atgagcgttt ccaaagtctt agttttcctc
     2161 atacaaagga aatgatgaag atttttcata aaaaatttgg cctgcataat tttagaacta
     2221 atcagctaga ggcgatcaat gctgcactgc ttggtgaaga ctgttttatc ctgatgccga
     2281 ctggaggtgg taagagtttg tgttaccagc tccctgcctg tgtttctcct ggggtcactg
     2341 ttgtcatttc tcccttgaga tcacttatcg tagatcaagt ccaaaagctg acttccttgg
     2401 atattccagc tacatatctg acaggtgata agactgactc agaagctaca aatatttacc
     2461 tccagttatc aaaaaaagac ccaatcataa aacttctata tgtcactcca gaaaagatct
     2521 gtgcaagtaa cagactcatt tctactctgg agaatctcta tgagaggaag ctcttggcac
     2581 gttttgttat tgatgaagca cattgtgtca gtcagtgggg acatgatttt cgtcaagatt
     2641 acaaaagaat gaatatgctt cgccagaagt ttccttctgt tccggtgatg gctcttacgg
     2701 ccacagctaa tcccagggta cagaaggaca tcctgactca gctgaagatt ctcagacctc
     2761 aggtgtttag catgagcttt aacagacata atctgaaata ctatgtatta ccgaaaaagc
     2821 ctaaaaaggt ggcatttgat tgcctagaat ggatcagaaa gcaccaccca tatgattcag
     2881 ggataattta ctgcctctcc aggcgagaat gtgacaccat ggctgacacg ttacagagag
     2941 atgggctcgc tgctcttgct taccatgctg gcctcagtga ttctgccaga gatgaagtgc
     3001 agcagaagtg gattaatcag gatggctgtc aggttatctg tgctacaatt gcatttggaa
     3061 tggggattga caaaccggac gtgcgatttg tgattcatgc atctctccct aaatctgtgg
     3121 agggttacta ccaagaatct ggcagagctg gaagagatgg ggaaatatct cactgcctgc
     3181 ttttctatac ctatcatgat gtgaccagac tgaaaagact tataatgatg gaaaaagatg
     3241 gaaaccatca tacaagagaa actcacttca ataatttgta tagcatggta cattactgtg
     3301 aaaatataac ggaatgcagg agaatacagc ttttggccta ctttggtgaa aatggattta
     3361 atcctgattt ttgtaagaaa cacccagatg tttcttgtga taattgctgt aaaacaaagg
     3421 attataaaac aagagatgtg actgacgatg tgaaaagtat tgtaagattt gttcaagaac
     3481 atagttcatc acaaggaatg agaaatataa aacatgtagg tccttctgga agatttacta
     3541 tgaatatgct ggtcgacatt ttcttgggga gtaagagtgc aaaaatccag tcaggtatat
     3601 ttggaaaagg atctgcttat tcacgacaca atgccgaaag actttttaaa aagctgatac
     3661 ttgacaagat tttggatgaa gacttatata tcaatgccaa tgaccaggcg atcgcttatg
     3721 tgatgctcgg aaataaagcc caaactgtac taaatggcaa tttaaaggta gactttatgg
     3781 aaacagaaaa ttccagcagt gtgaaaaaac aaaaagcgtt agtagcaaaa gtgtctcaga
     3841 gggaagagat ggttaaaaaa tgtcttggag aacttacaga agtctgcaaa tctctgggga
     3901 aagtttttgg tgtccattac ttcaatattt ttaataccgt cactctcaag aagcttgcag
     3961 aatctttatc ttctgatcct gaggttttgc ttcaaattga tggtgttact gaagacaaac
     4021 tggaaaaata tggtgcggaa gtgatttcag tattacagaa atactctgaa tggacatcgc
     4081 cagctgaaga cagttcccca gggataagcc tgtccagcag cagaggcccc ggaagaagtg
     4141 ccgctgagga gctcgacgag gaaatacccg tatcttccca ctactttgca agtaaaacca
     4201 gaaatgaaag gaagaggaaa aagatgccag cctcccaaag gtctaagagg agaaaaactg
     4261 cttccagtgg ttccaaggca aagggggggt ctgccacatg tagaaagata tcttccaaaa
     4321 cgaaatcctc cagcatcatt ggatccagtt cagcctcaca tacttctcaa gcgacatcag
     4381 gagccaatag caaattgggg attatggctc caccgaagcc tataaataga ccgtttctta
     4441 agccttcata tgcattctca taacaaccga atctcaatgt acatagaccc tctttcttgt
     4501 ttgtcagcat ctgaccatct gtgactataa agctgttatt cttgttatac catttgaagt
     4561 ttttactcgt ctctattaat atttaaataa atgctggggg gtgatagttc ttctttttaa
     4621 aataaacatt ttcttttgaa taagcatgtt ttgctgccgc tgcaagtgtt gtggccgttg
     4681 tttctcagaa cgtctgaggc agcagctgaa tcatctcagt gcaagagctt ctgagcataa
     4741 cacgaaaccc agaagccaaa ggaagagcca cgcgtgggcc cttgtgaaac taaagctttt
     4801 cgtgtaagac aacacaaaca aaatttaaag acaaatgacg gggaaaagag gagaaaatat
     4861 attacaaagg attagtatcc atcataccaa atacccgtga accagtcaga aacatcccag
     4921 ggggcaggtg gaccaaggat gtgaacaggc tagtctcaga agaagaaata cacatgctca
     4981 tggcccggca ctgtggctca cgcctgggat cccagcactt tgggaggccg aggcaggtgg
     5041 atcacgaggt caggagtttg agaccagcct gcccaacatg gtgaaacccc gtctctacta
     5101 aaaatacaaa aattagccag gcgtggtgta caggcacgcc tgtagtccca gctactcagg
     5161 aggctgaggc aagagaatcg cttgaaccca ggaggcggag gttgcagtga gccgagatcg
     5221 tgccactgca ctccagcctg ggtgacagag caagactccg tctcaaaaaa aaaaaaaaaa
     5281 aaagaaatat acatgctctg caaatatgtg aaaaaggtca atctccatga ataaaaatat
     5341 gataaaacca
//
