LOCUS       NM_000057               5240 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens BLM RecQ like helicase (BLM), transcript variant 1,
            mRNA.
ACCESSION   NM_000057
VERSION     NM_000057.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Silva,J.P.L., Donaires,F.S., Gutierrez-Rodrigues,F., Martins,D.J.,
            Carvalho,V.S., Santana,B.A., Cunha,R.L.G., Kajigaya,S.,
            Menck,C.F.M., Young,N.S., Kjeldsen,E. and Calado,R.T.
  TITLE     RecQ helicase expression in patients with telomeropathies
  JOURNAL   Mol Biol Rep 51 (1), 754 (2024)
   PUBMED   38874681
  REMARK    GeneRIF: RecQ helicase expression in patients with telomeropathies.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Tsukada,K., Jones,S.E., Bannister,J., Durin,M.A., Vendrell,I.,
            Fawkes,M., Fischer,R., Kessler,B.M., Chapman,J.R. and
            Blackford,A.N.
  TITLE     BLM and BRCA1-BARD1 coordinate complementary mechanisms of joint
            DNA molecule resolution
  JOURNAL   Mol Cell 84 (4), 640-658 (2024)
   PUBMED   38266639
  REMARK    GeneRIF: BLM and BRCA1-BARD1 coordinate complementary mechanisms of
            joint DNA molecule resolution.
REFERENCE   3  (bases 1 to 5240)
  AUTHORS   Liu,S., Atkinson,E., Paulucci-Holthauzen,A. and Wang,B.
  TITLE     A CK2 and SUMO-dependent, PML NB-involved regulatory mechanism
            controlling BLM ubiquitination and G-quadruplex resolution
  JOURNAL   Nat Commun 14 (1), 6111 (2023)
   PUBMED   37777511
  REMARK    GeneRIF: A CK2 and SUMO-dependent, PML NB-involved regulatory
            mechanism controlling BLM ubiquitination and G-quadruplex
            resolution.
            Erratum:[Nat Commun. 2024 Feb 5;15(1):1077. doi:
            10.1038/s41467-024-45551-1.. PMID: 38316763]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5240)
  AUTHORS   Danino,Y.M., Molitor,L., Rosenbaum-Cohen,T., Kaiser,S., Cohen,Y.,
            Porat,Z., Marmor-Kollet,H., Katina,C., Savidor,A., Rotkopf,R.,
            Ben-Isaac,E., Golani,O., Levin,Y., Monchaud,D., Hickson,I.D. and
            Hornstein,E.
  TITLE     BLM helicase protein negatively regulates stress granule formation
            through unwinding RNA G-quadruplex structures
  JOURNAL   Nucleic Acids Res 51 (17), 9369-9384 (2023)
   PUBMED   37503837
  REMARK    GeneRIF: BLM helicase protein negatively regulates stress granule
            formation through unwinding RNA G-quadruplex structures.
REFERENCE   5  (bases 1 to 5240)
  AUTHORS   Foucault,F., Vaury,C., Barakat,A., Thibout,D., Planchon,P.,
            Jaulin,C., Praz,F. and Amor-Gueret,M.
  TITLE     Characterization of a new BLM mutation associated with a
            topoisomerase II alpha defect in a patient with Bloom's syndrome
  JOURNAL   Hum Mol Genet 6 (9), 1427-1434 (1997)
   PUBMED   9285778
REFERENCE   6  (bases 1 to 5240)
  AUTHORS   Ellis,N.A., Groden,J., Ye,T.Z., Straughen,J., Lennon,D.J.,
            Ciocci,S., Proytcheva,M. and German,J.
  TITLE     The Bloom's syndrome gene product is homologous to RecQ helicases
  JOURNAL   Cell 83 (4), 655-666 (1995)
   PUBMED   7585968
REFERENCE   7  (bases 1 to 5240)
  AUTHORS   German,J., Roe,A.M., Leppert,M.F. and Ellis,N.A.
  TITLE     Bloom syndrome: an analysis of consanguineous families assigns the
            locus mutated to chromosome band 15q26.1
  JOURNAL   Proc Natl Acad Sci U S A 91 (14), 6669-6673 (1994)
   PUBMED   8022833
REFERENCE   8  (bases 1 to 5240)
  AUTHORS   Langer,K., Cunniff,C.M. and Kucine,N.
  TITLE     Bloom Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301572
REFERENCE   9  (bases 1 to 5240)
  AUTHORS   Turner,J.T., Brzezinski,J. and Dome,J.S.
  TITLE     Wilms Tumor Predisposition
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301471
REFERENCE   10 (bases 1 to 5240)
  AUTHORS   McDaniel,L.D. and Schultz,R.A.
  TITLE     Elevated sister chromatid exchange phenotype of Bloom syndrome
            cells is complemented by human chromosome 15
  JOURNAL   Proc Natl Acad Sci U S A 89 (17), 7968-7972 (1992)
   PUBMED   1518822
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD707743.1, BC062697.1,
            U39817.1, BC115030.1, BC034480.1 and AC124248.6.
            
            On Nov 22, 2018 this sequence version replaced NM_000057.3.
            
            Summary: The Bloom syndrome is an autosomal recessive disorder
            characterized by growth deficiency, microcephaly and
            immunodeficiency among others. It is caused by homozygous or
            compound heterozygous mutation in the gene encoding DNA helicase
            RecQ protein on chromosome 15q26. This Bloom-associated helicase
            unwinds a variety of DNA substrates including Holliday junction,
            and is involved in several pathways contributing to the maintenance
            of genome stability. Identification of pathogenic Bloom variants is
            required for heterozygote testing in at-risk families. [provided by
            RefSeq, May 2020].
            
            Transcript Variant: This variant (1) encodes the longest protein
            (isoform 1). Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034480.1, U39817.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000355112.8/ ENSP00000347232.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 CD707743.1         41-42
            3-25                BC062697.1         5-27
            26-1079             U39817.1           1-1054
            1080-4407           BC115030.1         1047-4374
            4408-4442           U39817.1           4383-4417
            4443-4530           BC034480.1         4392-4479
            4531-5240           AC124248.6         57937-58646
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q26.1"
     gene            1..5240
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="BLM RecQ like helicase"
                     /db_xref="GeneID:641"
                     /db_xref="HGNC:HGNC:1058"
                     /db_xref="MIM:604610"
     exon            1..95
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            96..197
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             100..4353
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /EC_number="5.6.2.4"
                     /note="isoform 1 is encoded by transcript variant 1; bloom
                     syndrome protein; recQ protein-like 3; Bloom syndrome RecQ
                     like helicase; DNA helicase, RecQ-like type 2; Bloom
                     syndrome, RecQ helicase-like; recQ-like DNA helicase BLM;
                     DNA 3'-5' helicase BLM"
                     /codon_start=1
                     /product="recQ-like DNA helicase BLM isoform 1"
                     /protein_id="NP_000048.1"
                     /db_xref="CCDS:CCDS10363.1"
                     /db_xref="GeneID:641"
                     /db_xref="HGNC:HGNC:1058"
                     /db_xref="MIM:604610"
                     /translation="MAAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSD
                     NNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRG
                     GSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDF
                     DTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQI
                     DLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLE
                     EAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDV
                     LSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLK
                     LLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSM
                     KELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAET
                     PRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDD
                     WEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQ
                     NYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLG
                     EDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGD
                     KTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAH
                     CVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMS
                     FNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLA
                     ALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEG
                     YYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYC
                     ENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFV
                     QEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLF
                     KKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKAL
                     VAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQ
                     IDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIP
                     VSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIG
                     SSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS"
     exon            198..898
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1058
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1186
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1319
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1981
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2173
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2174..2292
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2293..2406
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2505
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2654
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2761
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..2922
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2923..3118
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3119..3309
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3310..3457
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3458..3657
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3658..3850
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3851..3973
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            3974..4175
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     exon            4176..5240
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4416..4421
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="hexamer: TATAAA"
     polyA_site      4442
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="major polyA site"
     regulatory      4511..4516
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="hexamer: AATAAA"
     polyA_site      4536
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
     regulatory      5220..5225
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
                     /note="hexamer: AATAAA"
     polyA_site      5240
                     /gene="BLM"
                     /gene_synonym="BS; MGRISCE1; RECQ2; RECQL2; RECQL3"
ORIGIN      
        1 ccggcgggaa gtgagccagg gcttggcgcg gcggccgtgg ttgcggcgcg ggaagtttgg
       61 atcctggttc cgtccgctag gagtctgcgt gcgaggatta tggctgctgt tcctcaaaat
      121 aatctacagg agcaactaga acgtcactca gccagaacac ttaataataa attaagtctt
      181 tcaaaaccaa aattttcagg tttcactttt aaaaagaaaa catcttcaga taacaatgta
      241 tctgtaacta atgtgtcagt agcaaaaaca cctgtattaa gaaataaaga tgttaatgtt
      301 accgaagact tttccttcag tgaacctcta cccaacacca caaatcagca aagggtcaag
      361 gacttcttta aaaatgctcc agcaggacag gaaacacaga gaggtggatc aaaatcatta
      421 ttgccagatt tcttgcagac tccgaaggaa gttgtatgca ctacccaaaa cacaccaact
      481 gtaaagaaat cccgggatac tgctctcaag aaattagaat ttagttcttc accagattct
      541 ttaagtacca tcaatgattg ggatgatatg gatgactttg atacttctga gacttcaaaa
      601 tcatttgtta caccacccca aagtcacttt gtaagagtaa gcactgctca gaaatcaaaa
      661 aagggtaaga gaaacttttt taaagcacag ctttatacaa caaacacagt aaagactgat
      721 ttgcctccac cctcctctga aagcgagcaa atagatttga ctgaggaaca gaaggatgac
      781 tcagaatggt taagcagcga tgtgatttgc atcgatgatg gccccattgc tgaagtgcat
      841 ataaatgaag atgctcagga aagtgactct ctgaaaactc atttggaaga tgaaagagat
      901 aatagcgaaa agaagaagaa tttggaagaa gctgaattac attcaactga gaaagttcca
      961 tgtattgaat ttgatgatga tgattatgat acggattttg ttccaccttc tccagaagaa
     1021 attatttctg cttcttcttc ctcttcaaaa tgccttagta cgttaaagga ccttgacacc
     1081 tctgacagaa aagaggatgt tcttagcaca tcaaaagatc ttttgtcaaa acctgagaaa
     1141 atgagtatgc aggagctgaa tccagaaacc agcacagact gtgacgctag acagataagt
     1201 ttacagcagc agcttattca tgtgatggag cacatctgta aattaattga tactattcct
     1261 gatgataaac tgaaactttt ggattgtggg aacgaactgc ttcagcagcg gaacataaga
     1321 aggaaacttc taacggaagt agattttaat aaaagtgatg ccagtcttct tggctcattg
     1381 tggagataca ggcctgattc acttgatggc cctatggagg gtgattcctg ccctacaggg
     1441 aattctatga aggagttaaa tttttcacac cttccctcaa attctgtttc tcctggggac
     1501 tgtttactga ctaccaccct aggaaagaca ggattctctg ccaccaggaa gaatcttttt
     1561 gaaaggcctt tattcaatac ccatttacag aagtcctttg taagtagcaa ctgggctgaa
     1621 acaccaagac taggaaaaaa aaatgaaagc tcttatttcc caggaaatgt tctcacaagc
     1681 actgctgtga aagatcagaa taaacatact gcttcaataa atgacttaga aagagaaacc
     1741 caaccttcct atgatattga taattttgac atagatgact ttgatgatga tgatgactgg
     1801 gaagacataa tgcataattt agcagccagc aaatcttcca cagctgccta tcaacccatc
     1861 aaggaaggtc ggccaattaa atcagtatca gaaagacttt cctcagccaa gacagactgt
     1921 cttccagtgt catctactgc tcaaaatata aacttctcag agtcaattca gaattatact
     1981 gacaagtcag cacaaaattt agcatccaga aatctgaaac atgagcgttt ccaaagtctt
     2041 agttttcctc atacaaagga aatgatgaag atttttcata aaaaatttgg cctgcataat
     2101 tttagaacta atcagctaga ggcgatcaat gctgcactgc ttggtgaaga ctgttttatc
     2161 ctgatgccga ctggaggtgg taagagtttg tgttaccagc tccctgcctg tgtttctcct
     2221 ggggtcactg ttgtcatttc tcccttgaga tcacttatcg tagatcaagt ccaaaagctg
     2281 acttccttgg atattccagc tacatatctg acaggtgata agactgactc agaagctaca
     2341 aatatttacc tccagttatc aaaaaaagac ccaatcataa aacttctata tgtcactcca
     2401 gaaaagatct gtgcaagtaa cagactcatt tctactctgg agaatctcta tgagaggaag
     2461 ctcttggcac gttttgttat tgatgaagca cattgtgtca gtcagtgggg acatgatttt
     2521 cgtcaagatt acaaaagaat gaatatgctt cgccagaagt ttccttctgt tccggtgatg
     2581 gctcttacgg ccacagctaa tcccagggta cagaaggaca tcctgactca gctgaagatt
     2641 ctcagacctc aggtgtttag catgagcttt aacagacata atctgaaata ctatgtatta
     2701 ccgaaaaagc ctaaaaaggt ggcatttgat tgcctagaat ggatcagaaa gcaccaccca
     2761 tatgattcag ggataattta ctgcctctcc aggcgagaat gtgacaccat ggctgacacg
     2821 ttacagagag atgggctcgc tgctcttgct taccatgctg gcctcagtga ttctgccaga
     2881 gatgaagtgc agcagaagtg gattaatcag gatggctgtc aggttatctg tgctacaatt
     2941 gcatttggaa tggggattga caaaccggac gtgcgatttg tgattcatgc atctctccct
     3001 aaatctgtgg agggttacta ccaagaatct ggcagagctg gaagagatgg ggaaatatct
     3061 cactgcctgc ttttctatac ctatcatgat gtgaccagac tgaaaagact tataatgatg
     3121 gaaaaagatg gaaaccatca tacaagagaa actcacttca ataatttgta tagcatggta
     3181 cattactgtg aaaatataac ggaatgcagg agaatacagc ttttggccta ctttggtgaa
     3241 aatggattta atcctgattt ttgtaagaaa cacccagatg tttcttgtga taattgctgt
     3301 aaaacaaagg attataaaac aagagatgtg actgacgatg tgaaaagtat tgtaagattt
     3361 gttcaagaac atagttcatc acaaggaatg agaaatataa aacatgtagg tccttctgga
     3421 agatttacta tgaatatgct ggtcgacatt ttcttgggga gtaagagtgc aaaaatccag
     3481 tcaggtatat ttggaaaagg atctgcttat tcacgacaca atgccgaaag actttttaaa
     3541 aagctgatac ttgacaagat tttggatgaa gacttatata tcaatgccaa tgaccaggcg
     3601 atcgcttatg tgatgctcgg aaataaagcc caaactgtac taaatggcaa tttaaaggta
     3661 gactttatgg aaacagaaaa ttccagcagt gtgaaaaaac aaaaagcgtt agtagcaaaa
     3721 gtgtctcaga gggaagagat ggttaaaaaa tgtcttggag aacttacaga agtctgcaaa
     3781 tctctgggga aagtttttgg tgtccattac ttcaatattt ttaataccgt cactctcaag
     3841 aagcttgcag aatctttatc ttctgatcct gaggttttgc ttcaaattga tggtgttact
     3901 gaagacaaac tggaaaaata tggtgcggaa gtgatttcag tattacagaa atactctgaa
     3961 tggacatcgc cagctgaaga cagttcccca gggataagcc tgtccagcag cagaggcccc
     4021 ggaagaagtg ccgctgagga gctcgacgag gaaatacccg tatcttccca ctactttgca
     4081 agtaaaacca gaaatgaaag gaagaggaaa aagatgccag cctcccaaag gtctaagagg
     4141 agaaaaactg cttccagtgg ttccaaggca aagggggggt ctgccacatg tagaaagata
     4201 tcttccaaaa cgaaatcctc cagcatcatt ggatccagtt cagcctcaca tacttctcaa
     4261 gcgacatcag gagccaatag caaattgggg attatggctc caccgaagcc tataaataga
     4321 ccgtttctta agccttcata tgcattctca taacaaccga atctcaatgt acatagaccc
     4381 tctttcttgt ttgtcagcat ctgaccatct gtgactataa agctgttatt cttgttatac
     4441 catttgaagt ttttactcgt ctctattaat atttaaataa atgctggggg gtgatagttc
     4501 ttctttttaa aataaacatt ttcttttgaa taagcatgtt ttgctgccgc tgcaagtgtt
     4561 gtggccgttg tttctcagaa cgtctgaggc agcagctgaa tcatctcagt gcaagagctt
     4621 ctgagcataa cacgaaaccc agaagccaaa ggaagagcca cgcgtgggcc cttgtgaaac
     4681 taaagctttt cgtgtaagac aacacaaaca aaatttaaag acaaatgacg gggaaaagag
     4741 gagaaaatat attacaaagg attagtatcc atcataccaa atacccgtga accagtcaga
     4801 aacatcccag ggggcaggtg gaccaaggat gtgaacaggc tagtctcaga agaagaaata
     4861 cacatgctca tggcccggca ctgtggctca cgcctgggat cccagcactt tgggaggccg
     4921 aggcaggtgg atcacgaggt caggagtttg agaccagcct gcccaacatg gtgaaacccc
     4981 gtctctacta aaaatacaaa aattagccag gcgtggtgta caggcacgcc tgtagtccca
     5041 gctactcagg aggctgaggc aagagaatcg cttgaaccca ggaggcggag gttgcagtga
     5101 gccgagatcg tgccactgca ctccagcctg ggtgacagag caagactccg tctcaaaaaa
     5161 aaaaaaaaaa aaagaaatat acatgctctg caaatatgtg aaaaaggtca atctccatga
     5221 ataaaaatat gataaaacca
//
