LOCUS       NM_004327               6783 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens BCR activator of RhoGEF and GTPase (BCR), transcript
            variant 1, mRNA.
ACCESSION   NM_004327
VERSION     NM_004327.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Chaudhary,P., Chaudhary,S., Patel,F., Patel,S., Vaishnani,T.,
            Trivedi,N., Patel,D., Sonagara,T., Hirapara,A., Vyas,K., Patel,L.,
            Kumar,R., Chakraborty,N., Sharma,D., Suthar,J., Kamdar,P.,
            Jajodia,E., Ahmad,F. and Arora,N.
  TITLE     Validation of a novel NGS based BCR::ABL1 kinase domain mutation
            detection assay in Indian cohort
  JOURNAL   Sci Rep 14 (1), 15745 (2024)
   PUBMED   38977756
  REMARK    GeneRIF: Validation of a novel NGS based BCR::ABL1 kinase domain
            mutation detection assay in Indian cohort.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Bastian,L., Beder,T., Barz,M.J., Bendig,S., Bartsch,L., Walter,W.,
            Wolgast,N., Brandl,B., Rohrandt,C., Hansen,B.T., Hartmann,A.M.,
            Iben,K., Das Gupta,D., Denker,M., Zimmermann,J., Wittig,M.,
            Chitadze,G., Neumann,M., Schneller,F., Fiedler,W., Steffen,B.,
            Stelljes,M., Faul,C., Schwartz,S., Muller,F.J., Cario,G.,
            Harder,L., Haferlach,C., Pfeifer,H., Gokbuget,N., Bruggemann,M. and
            Baldus,C.D.
  TITLE     Developmental trajectories and cooperating genomic events define
            molecular subtypes of BCR::ABL1-positive ALL
  JOURNAL   Blood 143 (14), 1391-1398 (2024)
   PUBMED   38153913
  REMARK    GeneRIF: Developmental trajectories and cooperating genomic events
            define molecular subtypes of BCR::ABL1-positive ALL.
REFERENCE   3  (bases 1 to 6783)
  AUTHORS   Feng,H., Fu,Y., Cui,Z., Zhou,M., Zhang,L., Gao,Z., Ma,S. and
            Chen,C.
  TITLE     Histone demethylase PHF8 facilitates the development of chronic
            myeloid leukaemia by directly targeting BCR::ABL1
  JOURNAL   Br J Haematol 202 (6), 1178-1191 (2023)
   PUBMED   37469124
  REMARK    GeneRIF: Histone demethylase PHF8 facilitates the development of
            chronic myeloid leukaemia by directly targeting BCR::ABL1.
            Erratum:[Br J Haematol. 2024 May;204(5):2134-2135. doi:
            10.1111/bjh.19415.. PMID: 38567568]
REFERENCE   4  (bases 1 to 6783)
  AUTHORS   Deregowska,A., Pepek,M., Solarska,I., Machnicki,M.M., Pruszczyk,K.,
            Dudzinski,M., Niesiobedzka-Krezel,J., Seferynska,I., Sawicki,W.,
            Wnuk,M. and Stoklosa,T.
  TITLE     The interplay between telomeric complex members and BCR::ABL1
            oncogenic tyrosine kinase in the maintenance of telomere length in
            chronic myeloid leukemia
  JOURNAL   J Cancer Res Clin Oncol 149 (10), 7103-7112 (2023)
   PUBMED   36871092
  REMARK    GeneRIF: The interplay between telomeric complex members and
            BCR::ABL1 oncogenic tyrosine kinase in the maintenance of telomere
            length in chronic myeloid leukemia.
REFERENCE   5  (bases 1 to 6783)
  AUTHORS   Park,B., Kim,J., Kim,M., Cho,Y.U., Hwang,S.H., Jang,S., Seo,E.J.,
            Choi,E.J. and Park,C.J.
  TITLE     Emergence of a BCR::ABL1 rearrangement following a uniquely complex
            clonal evolution pattern in a patient undergoing Fms-like tyrosine
            kinase 3 inhibitor therapy for acute myeloid leukemia
  JOURNAL   Int J Lab Hematol 45 (2), e24-e27 (2023)
   PUBMED   36180046
  REMARK    GeneRIF: Emergence of a BCR::ABL1 rearrangement following a
            uniquely complex clonal evolution pattern in a patient undergoing
            Fms-like tyrosine kinase 3 inhibitor therapy for acute myeloid
            leukemia.
REFERENCE   6  (bases 1 to 6783)
  AUTHORS   Mills,K.I., Sproul,A.M., Ogilvie,D., Elvin,P., Leibowitz,D. and
            Burnett,A.K.
  TITLE     Amplification and sequencing of genomic breakpoints located within
            the M-bcr region by Vectorette-mediated polymerase chain reaction
  JOURNAL   Leukemia 6 (5), 481-483 (1992)
   PUBMED   1317490
REFERENCE   7  (bases 1 to 6783)
  AUTHORS   Uberbacher,E.C. and Mural,R.J.
  TITLE     Locating protein-coding regions in human DNA sequences by a
            multiple sensor-neural network approach
  JOURNAL   Proc Natl Acad Sci U S A 88 (24), 11261-11265 (1991)
   PUBMED   1763041
REFERENCE   8  (bases 1 to 6783)
  AUTHORS   Maru,Y. and Witte,O.N.
  TITLE     The BCR gene encodes a novel serine/threonine kinase activity
            within a single exon
  JOURNAL   Cell 67 (3), 459-468 (1991)
   PUBMED   1657398
REFERENCE   9  (bases 1 to 6783)
  AUTHORS   Pendergast,A.M., Muller,A.J., Havlik,M.H., Maru,Y. and Witte,O.N.
  TITLE     BCR sequences essential for transformation by the BCR-ABL oncogene
            bind to the ABL SH2 regulatory domain in a
            non-phosphotyrosine-dependent manner
  JOURNAL   Cell 66 (1), 161-171 (1991)
   PUBMED   1712671
REFERENCE   10 (bases 1 to 6783)
  AUTHORS   Jurka,J. and Milosavljevic,A.
  TITLE     Reconstruction and analysis of human Alu genes
  JOURNAL   J Mol Evol 32 (2), 105-121 (1991)
   PUBMED   1706781
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U07000.1, BC066122.1,
            BQ422888.1 and X02596.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004327.3.
            
            Summary: A reciprocal translocation between chromosomes 22 and 9
            produces the Philadelphia chromosome, which is often found in
            patients with chronic myelogenous leukemia. The chromosome 22
            breakpoint for this translocation is located within the BCR gene.
            The translocation produces a fusion protein which is encoded by
            sequence from both BCR and ABL, the gene at the chromosome 9
            breakpoint. Although the BCR-ABL fusion protein has been
            extensively studied, the function of the normal BCR gene product is
            not clear. The unregulated tyrosine kinase activity of BCR-ABL1
            contributes to the immortality of leukaemic cells. The BCR protein
            has serine/threonine kinase activity and is a GTPase-activating
            protein for p21rac and other kinases. Two transcript variants
            encoding different isoforms have been found for this gene.[provided
            by RefSeq, Jan 2020].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.226032.1, BC066122.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000305877.13/ ENSP00000303507.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1731              U07000.1           14587-16317
            1732-2838           BC066122.1         1707-2813
            2839-3383           BQ422888.1         109-653
            3384-3784           X02596.1           3420-3820
            3785-4695           BC066122.1         3760-4670
            4696-6783           U07000.1           150028-152115
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q11.23"
     gene            1..6783
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /note="BCR activator of RhoGEF and GTPase"
                     /db_xref="GeneID:613"
                     /db_xref="HGNC:HGNC:1014"
                     /db_xref="MIM:151410"
     exon            1..1731
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     CDS             453..4268
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1; renal
                     carcinoma antigen NY-REN-26; BCR/FGFR1 chimera protein;
                     FGFR1/BCR chimera protein; BCR, RhoGEF and GTPase
                     activating protein; breakpoint cluster region"
                     /codon_start=1
                     /product="breakpoint cluster region protein isoform 1"
                     /protein_id="NP_004318.3"
                     /db_xref="CCDS:CCDS13806.1"
                     /db_xref="GeneID:613"
                     /db_xref="HGNC:HGNC:1014"
                     /db_xref="MIM:151410"
                     /translation="MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRR
                     LEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPA
                     DGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFER
                     IRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHG
                     AGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEY
                     QPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSS
                     NENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSFDSSSPPTPQCHKRH
                     RHCPVVVSEATIVGVRKTGQIWPNDGEGAFHGDADGSFGTPPGYGCAADRAEEQRRHQ
                     DGLPYIDDSPSSSPHLSSKGRGSRDALVSGALESTKASELDLEKGLEMRKWVLSGILA
                     SEETYLSHLEALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFP
                     RVQQWSHQQRVGDLFQKLASQLGVYRAFVDNYGVAMEMAEKCCQANAQFAEISENLRA
                     RSNKDAKDPTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTPASHPDHPLLQDALRISQ
                     NFLSSINEEITPRRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKL
                     KKQSGGKTQQYDCKWYIPLTDLSFQMVDELEAVPNIPLVPDEELDALKIKISQIKNDI
                     QREKRANKGSKATERLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAE
                     WRENIREQQKKCFRSFSLTSVELQMLTNSCVKLQTVHSIPLTINKEDDESPGLYGFLN
                     VIVHSATGFKQSSNLYCTLEVDSFGYFVNKAKTRVYRDTAEPNWNEEFEIELEGSQTL
                     RILCYEKCYNKTKIPKEDGESTDRLMGKGQVQLDPQALQDRDWQRTVIAMNGIEVKLS
                     VKFNSREFSLKRMPSRKQTGVFGVKIAVVTKRERSKVPYIVRQCVEEIERRGMEEVGI
                     YRVSGVATDIQALKAAFDVNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDEFYP
                     NFAEGIALSDPVAKESCMLNLLLSLPEANLLTFLFLLDHLKRVAEKEAVNKMSLHNLA
                     TVFGPTLLRPSEKESKLPANPSQPITMTDSWSLEVMSQVQVLLYFLQLEAIPAPDSKR
                     QSILFSTEV"
     exon            1732..1913
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..2018
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2204
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2312
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..2373
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2426
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2427..2567
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2568..2689
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2690..2858
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2859..2978
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            2979..3054
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3055..3159
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3234
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3235..3332
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3333..3464
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3465..3524
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3525..3634
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3635..3774
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3775..3909
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            3910..4015
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            4016..4178
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     exon            4179..6783
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4678..4683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /note="hexamer: AATAAA"
     polyA_site      4696
                     /gene="BCR"
                     /gene_synonym="ALL; BCR1; CML; D22S11; D22S662; PHL"
                     /note="major polyA site"
ORIGIN      
        1 gcagagtgcg ggccgggcgg gagtgcggcg agagccggct ggctgagctt agcgtccgag
       61 gaggcggcgg cggcggcggc ggcacggcgg cggcggggct gtggggcggt gcggaagcga
      121 gaggcgagga gcgcgcgggc cgtggccaga gtctggcggc ggcctggcgg agcggagagc
      181 agcgcccgcg cctcgccgtg cggaggagcc ccgcacacaa tagcggcgcg cgcagcccgc
      241 gcccttcccc ccggcgcgcc ccgccccgcg cgccgagcgc cccgctccgc ctcacctgcc
      301 accagggagt gggcgggcat tgttcgccgc cgccgccgcc gcgcgggcca tgggggccgc
      361 ccggcgcccg gggccgggct ggcgaggcgc cgcgccgccg ctgagacggg ccccgcgcgc
      421 agcccggcgg cgcaggtaag gccggccgcg ccatggtgga cccggtgggc ttcgcggagg
      481 cgtggaaggc gcagttcccg gactcagagc ccccgcgcat ggagctgcgc tcagtgggcg
      541 acatcgagca ggagctggag cgctgcaagg cctccattcg gcgcctggag caggaggtga
      601 accaggagcg cttccgcatg atctacctgc agacgttgct ggccaaggaa aagaagagct
      661 atgaccggca gcgatggggc ttccggcgcg cggcgcaggc ccccgacggc gcctccgagc
      721 cccgagcgtc cgcgtcgcgc ccgcagccag cgcccgccga cggagccgac ccgccgcccg
      781 ccgaggagcc cgaggcccgg cccgacggcg agggttctcc gggtaaggcc aggcccggga
      841 ccgcccgcag gcccggggca gccgcgtcgg gggaacggga cgaccgggga ccccccgcca
      901 gcgtggcggc gctcaggtcc aacttcgagc ggatccgcaa gggccatggc cagcccgggg
      961 cggacgccga gaagcccttc tacgtgaacg tcgagtttca ccacgagcgc ggcctggtga
     1021 aggtcaacga caaagaggtg tcggaccgca tcagctccct gggcagccag gccatgcaga
     1081 tggagcgcaa aaagtcccag cacggcgcgg gctcgagcgt gggggatgca tccaggcccc
     1141 cttaccgggg acgctcctcg gagagcagct gcggcgtcga cggcgactac gaggacgccg
     1201 agttgaaccc ccgcttcctg aaggacaacc tgatcgacgc caatggcggt agcaggcccc
     1261 cttggccgcc cctggagtac cagccctacc agagcatcta cgtcgggggc atgatggaag
     1321 gggagggcaa gggcccgctc ctgcgcagcc agagcacctc tgagcaggag aagcgcctta
     1381 cctggccccg caggtcctac tccccccgga gttttgagga ttgcggaggc ggctataccc
     1441 cggactgcag ctccaatgag aacctcacct ccagcgagga ggacttctcc tctggccagt
     1501 ccagccgcgt gtccccaagc cccaccacct accgcatgtt ccgggacaaa agccgctctc
     1561 cctcgcagaa ctcgcaacag tccttcgaca gcagcagtcc ccccacgccg cagtgccata
     1621 agcggcaccg gcactgcccg gttgtcgtgt ccgaggccac catcgtgggc gtccgcaaga
     1681 ccgggcagat ctggcccaac gatggcgagg gcgccttcca tggagacgca gatggctcgt
     1741 tcggaacacc acctggatac ggctgcgctg cagaccgggc agaggagcag cgccggcacc
     1801 aagatgggct gccctacatt gatgactcgc cctcctcatc gccccacctc agcagcaagg
     1861 gcaggggcag ccgggatgcg ctggtctcgg gagccctgga gtccactaaa gcgagtgagc
     1921 tggacttgga aaagggcttg gagatgagaa aatgggtcct gtcgggaatc ctggctagcg
     1981 aggagactta cctgagccac ctggaggcac tgctgctgcc catgaagcct ttgaaagccg
     2041 ctgccaccac ctctcagccg gtgctgacga gtcagcagat cgagaccatc ttcttcaaag
     2101 tgcctgagct ctacgagatc cacaaggagt tctatgatgg gctcttcccc cgcgtgcagc
     2161 agtggagcca ccagcagcgg gtgggcgacc tcttccagaa gctggccagc cagctgggtg
     2221 tgtaccgggc cttcgtggac aactacggag ttgccatgga aatggctgag aagtgctgtc
     2281 aggccaatgc tcagtttgca gaaatctccg agaacctgag agccagaagc aacaaagatg
     2341 ccaaggatcc aacgaccaag aactctctgg aaactctgct ctacaagcct gtggaccgtg
     2401 tgacgaggag cacgctggtc ctccatgact tgctgaagca cactcctgcc agccaccctg
     2461 accacccctt gctgcaggac gccctccgca tctcacagaa cttcctgtcc agcatcaatg
     2521 aggagatcac accccgacgg cagtccatga cggtgaagaa gggagagcac cggcagctgc
     2581 tgaaggacag cttcatggtg gagctggtgg agggggcccg caagctgcgc cacgtcttcc
     2641 tgttcaccga cctgcttctc tgcaccaagc tcaagaagca gagcggaggc aaaacgcagc
     2701 agtatgactg caaatggtac attccgctca cggatctcag cttccagatg gtggatgaac
     2761 tggaggcagt gcccaacatc cccctggtgc ccgatgagga gctggacgct ttgaagatca
     2821 agatctccca gatcaagaat gacatccaga gagagaagag ggcgaacaag ggcagcaagg
     2881 ctacggagag gctgaagaag aagctgtcgg agcaggagtc actgctgctg cttatgtctc
     2941 ccagcatggc cttcagggtg cacagccgca acggcaagag ttacacgttc ctgatctcct
     3001 ctgactatga gcgtgcagag tggagggaga acatccggga gcagcagaag aagtgtttca
     3061 gaagcttctc cctgacatcc gtggagctgc agatgctgac caactcgtgt gtgaaactcc
     3121 agactgtcca cagcattccg ctgaccatca ataaggaaga tgatgagtct ccggggctct
     3181 atgggtttct gaatgtcatc gtccactcag ccactggatt taagcagagt tcaaatctgt
     3241 actgcaccct ggaggtggat tcctttgggt attttgtgaa taaagcaaag acgcgcgtct
     3301 acagggacac agctgagcca aactggaacg aggaatttga gatagagctg gagggctccc
     3361 agaccctgag gatactgtgc tatgaaaagt gttacaacaa gacgaagatc cccaaggagg
     3421 acggcgagag cacggacaga ctcatgggga agggccaggt ccagctggac ccgcaggccc
     3481 tgcaggacag agactggcag cgcaccgtca tcgccatgaa tgggatcgaa gtaaagctct
     3541 cggtcaagtt caacagcagg gagttcagct tgaagaggat gccgtcccga aaacagacag
     3601 gggtcttcgg agtcaagatt gctgtggtca ccaagagaga gaggtccaag gtgccctaca
     3661 tcgtgcgcca gtgcgtggag gagatcgagc gccgaggcat ggaggaggtg ggcatctacc
     3721 gcgtgtccgg tgtggccacg gacatccagg cactgaaggc agccttcgac gtcaataaca
     3781 aggacgtgtc ggtgatgatg agcgagatgg acgtgaacgc catcgcaggc acgctgaagc
     3841 tgtacttccg tgagctgccc gagcccctct tcactgacga gttctacccc aacttcgcag
     3901 agggcatcgc tctttcagac ccggttgcaa aggagagctg catgctcaac ctgctgctgt
     3961 ccctgccgga ggccaacctg ctcaccttcc ttttccttct ggaccacctg aaaagggtgg
     4021 cagagaagga ggcagtcaat aagatgtccc tgcacaacct cgccacggtc tttggcccca
     4081 cgctgctccg gccctccgag aaggagagca agctccctgc caaccccagc cagcctatca
     4141 ccatgactga cagctggtcc ttggaggtca tgtcccaggt ccaggtgctg ctgtacttcc
     4201 tgcagctgga ggccatccct gccccggaca gcaagagaca gagcatcctg ttctccaccg
     4261 aagtctaaag gtcccagtcc atctcctgga ggcggacaga tggcctggaa acctctggct
     4321 aatcgggcca tccgtagagc gggaaccttc ctgaggtgtc cttgggccac ccccaagtgt
     4381 tgggccatct gccaagagac agcgacccaa agccgaagga caggtggcct ggaaagatcc
     4441 cgcccaggtc tgggagcccc aggctggcct cagactgtgg ttttttatgt ggccacctga
     4501 gggcgcccca agccagttca tctcggagtc caggcctggc cctgggagac agggtgaagg
     4561 gagtggtttt tatgaactta acttagagtc taaaagattt ctactggatc acttgtcaag
     4621 atgcgccctc tctggggaga agggaacgtg actggattcc ctcactgttg tatcttgaat
     4681 aaacgctgct gcttcatcct gtgggggccg tggccctgtc cctgtgtggg tggggcctct
     4741 tccatttccc tgacttagaa accacactcc acttctaaca gggtttgaga ggcttagtca
     4801 gcactgggta gcgttttgac tccattcttg gctttctttt tctttccaga aggatttttg
     4861 tgcagaaatg ggtcttttgt tgccatgtta gtcctccttg gaaggcagct cagaaggcct
     4921 gtgaaatgtc aggggacagg acccccaggg agggaacccc aggctacgca ctttagggtt
     4981 cgttctccag ggagagcgac ctcgtccccc gatcctgacc gcccttccgg cccacgctct
     5041 cctgtttggc ttccacaggc ctggacttct ctggcttctc tgcccacaca ctccctgccc
     5101 ccagtgtccc tgcccctgcc ccagcacagg tgacttcatt tctgtcctct cagctcagtg
     5161 gactcgctca acttttgtat aagtctccac ttggtggcag cagcttgctg atgacttgtt
     5221 ttaaaacttt catcctaaat aaccttttga tacttgaata tttgtaagtt ttatacatag
     5281 tttctaattt ttttccgaac agatccagat acctaataag atgctggaat gtaatccctg
     5341 gacaatccgt gtcctggcag catttggtct tcctctaagc gcctggctcc gctgttctca
     5401 ggagtgggtt ctgaagtctc tggagaacag gatacgtgga gggttaggaa ggggccaggc
     5461 ctagagacgg gagactccct cccggagcag gtggaggcac aggaccattc gctaccccat
     5521 ctgccgacac ctgcggggga gcccaggcat tctttgtaaa ccctcctgac cacctggctc
     5581 aaagaaaaca gaagcatgga gaccgccaag tattttcaag aaataacccc atgaatattc
     5641 catcactttt ttagaaagag gggcttgggg caggcagagg agagaaggga gagcaaactg
     5701 agagccaagt ttccacacgg tcctgcagga ggagaggatg cagctgccca gagggaagca
     5761 ggatcacatt taaggaagtg tgtggggtcc ctggatgaca ccagcaccca gtgcggctct
     5821 gtctggcacc cgctcccaag gtgggaggag tgggtgtccc ctgtgtgtca gtgggcagct
     5881 cctgctgaac ccgcagctca ctagggagcc tgacagtggg gccatgcgcc tgacactcct
     5941 ctctgcttgt ggacctggca aggcagggag cagaaaacag ccacttgaag gctttctgtc
     6001 tgcgtctgtg tgcagtgtgg atttagttgt gcttttttct tgctgggaga gcacagccac
     6061 catttacaag cagtgtcacc gtcgtgggtg gcgaggacag aacaggagcc tctgctctct
     6121 gtacctatct gggcccggtg ggctcccttg tcctggcttc catctctgtc tcagcgacca
     6181 ttcagccctg cgcaggaaca cgtgttgctt agaaaagcca aatccagcct tgtctctgcc
     6241 tcctctggtc tcatgatgtg catctgttac cttgaaactg gaaaccagtc tatcaatgtc
     6301 tgtgccaatt ttttattccc tccccaacct ccttccccat acgacttttt atttatgtag
     6361 gatgtgtgct gtctaatgat gggatgacca cacttttcca tgttctaaaa gtgctcctct
     6421 cccgcagggt cccagggctg gtggttgctt tgggtctaca gctacgtctt acccacctcc
     6481 tgcctcaaca gcctgtgtgg tggcaaagcc ggtgtggggc tggggaacgc agcgttctcc
     6541 aggaggggga cctggctctc cttctgcaat gcaggcgaag gcctagatgc cagtgtgacc
     6601 tcccacaagg cgtggcttcc agactccccg gccggaagtg atgctttttt gccttgggcc
     6661 ctgggtttga agcagcctgg ctttctcttg gtaagtggct ggtgtcttag cagctgcaat
     6721 ctgagctcag ccacctacac accaccgtgg ccgacacttt cattaaaaag tttcctgaga
     6781 cga
//
