LOCUS       NM_014739               7494 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens BCL2 associated transcription factor 1 (BCLAF1),
            transcript variant 1, mRNA.
ACCESSION   NM_014739
VERSION     NM_014739.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Li,Y., Sun,Y., Jia,B., Ma,Z. and Zhou,R.
  TITLE     MED23 depletion induces premature senescence in NSCLC cells by
            interacting with BCLAF1 and then suppressing NUPR1 expression
  JOURNAL   Biochem Biophys Res Commun 734, 150754 (2024)
   PUBMED   39366174
  REMARK    GeneRIF: MED23 depletion induces premature senescence in NSCLC
            cells by interacting with BCLAF1 and then suppressing NUPR1
            expression.
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Zhou,Y., Ran,X. and Han,M.
  TITLE     BCLAF1 is Expressed as a Potential Anti-oncogene in Bile Duct
            Cancer
  JOURNAL   Biochem Genet 62 (5), 3681-3694 (2024)
   PUBMED   38198022
  REMARK    GeneRIF: BCLAF1 is Expressed as a Potential Anti-oncogene in Bile
            Duct Cancer.
REFERENCE   3  (bases 1 to 7494)
  AUTHORS   Chen,J., Wang,H., Xu,J., Chen,E., Meng,Q., Wang,J., Xiang,H.,
            Zhou,W., Shan,G., Ju,Z. and Song,Z.
  TITLE     CircZFR promotes colorectal cancer progression via stabilizing
            BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
  JOURNAL   Sci China Life Sci 67 (9), 1881-1898 (2024)
   PUBMED   38805063
  REMARK    GeneRIF: CircZFR promotes colorectal cancer progression via
            stabilizing BCLAF1 and regulating the miR-3127-5p/RTKN2 axis.
REFERENCE   4  (bases 1 to 7494)
  AUTHORS   Zhang,P., Zhang,W., Wang,X., Li,L., Lin,Y., Wu,N., Mao,R., Lin,J.,
            Kang,M. and Ding,C.
  TITLE     BCLAF1 drives esophageal squamous cell carcinoma progression
            through regulation of YTHDF2-dependent SIX1 mRNA degradation
  JOURNAL   Cancer Lett 591, 216874 (2024)
   PUBMED   38636894
  REMARK    GeneRIF: BCLAF1 drives esophageal squamous cell carcinoma
            progression through regulation of YTHDF2-dependent SIX1 mRNA
            degradation.
REFERENCE   5  (bases 1 to 7494)
  AUTHORS   Yu,Z., Wu,X., Zhu,J., Yan,H., Li,Y., Zhang,H., Zhong,Y., Lin,M.,
            Ye,G., Li,X., Jin,J., Li,K., Wang,J., Zhuang,H., Lin,T., He,J.,
            Lu,C., Xu,Z., Zhang,X., Li,H. and Jin,X.
  TITLE     BCLAF1 binds SPOP to stabilize PD-L1 and promotes the development
            and immune escape of hepatocellular carcinoma
  JOURNAL   Cell Mol Life Sci 81 (1), 82 (2024)
   PUBMED   38340178
  REMARK    GeneRIF: BCLAF1 binds SPOP to stabilize PD-L1 and promotes the
            development and immune escape of hepatocellular carcinoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7494)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   7  (bases 1 to 7494)
  AUTHORS   Haraguchi,T., Holaska,J.M., Yamane,M., Koujin,T., Hashiguchi,N.,
            Mori,C., Wilson,K.L. and Hiraoka,Y.
  TITLE     Emerin binding to Btf, a death-promoting transcriptional repressor,
            is disrupted by a missense mutation that causes Emery-Dreifuss
            muscular dystrophy
  JOURNAL   Eur J Biochem 271 (5), 1035-1045 (2004)
   PUBMED   15009215
  REMARK    GeneRIF: These results suggest that Btf localization is regulated
            by apoptotic signals, and that loss of emerin binding to Btf may be
            relevant to muscle wasting in Emery-Dreifuss muscular dystrophy.
REFERENCE   8  (bases 1 to 7494)
  AUTHORS   Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
            Gilbert,J.G., Clamp,M.E., Bethel,G., Milne,S., Ainscough,R.,
            Almeida,J.P., Ambrose,K.D., Andrews,T.D., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Bailey,J., Banerjee,R., Barker,D.J.,
            Barlow,K.F., Bates,K., Beare,D.M., Beasley,H., Beasley,O.,
            Bird,C.P., Blakey,S., Bray-Allen,S., Brook,J., Brown,A.J.,
            Brown,J.Y., Burford,D.C., Burrill,W., Burton,J., Carder,C.,
            Carter,N.P., Chapman,J.C., Clark,S.Y., Clark,G., Clee,C.M.,
            Clegg,S., Cobley,V., Collier,R.E., Collins,J.E., Colman,L.K.,
            Corby,N.R., Coville,G.J., Culley,K.M., Dhami,P., Davies,J.,
            Dunn,M., Earthrowl,M.E., Ellington,A.E., Evans,K.A., Faulkner,L.,
            Francis,M.D., Frankish,A., Frankland,J., French,L., Garner,P.,
            Garnett,J., Ghori,M.J., Gilby,L.M., Gillson,C.J., Glithero,R.J.,
            Grafham,D.V., Grant,M., Gribble,S., Griffiths,C., Griffiths,M.,
            Hall,R., Halls,K.S., Hammond,S., Harley,J.L., Hart,E.A.,
            Heath,P.D., Heathcott,R., Holmes,S.J., Howden,P.J., Howe,K.L.,
            Howell,G.R., Huckle,E., Humphray,S.J., Humphries,M.D., Hunt,A.R.,
            Johnson,C.M., Joy,A.A., Kay,M., Keenan,S.J., Kimberley,A.M.,
            King,A., Laird,G.K., Langford,C., Lawlor,S., Leongamornlert,D.A.,
            Leversha,M., Lloyd,C.R., Lloyd,D.M., Loveland,J.E., Lovell,J.,
            Martin,S., Mashreghi-Mohammadi,M., Maslen,G.L., Matthews,L.,
            McCann,O.T., McLaren,S.J., McLay,K., McMurray,A., Moore,M.J.,
            Mullikin,J.C., Niblett,D., Nickerson,T., Novik,K.L., Oliver,K.,
            Overton-Larty,E.K., Parker,A., Patel,R., Pearce,A.V., Peck,A.I.,
            Phillimore,B., Phillips,S., Plumb,R.W., Porter,K.M., Ramsey,Y.,
            Ranby,S.A., Rice,C.M., Ross,M.T., Searle,S.M., Sehra,H.K.,
            Sheridan,E., Skuce,C.D., Smith,S., Smith,M., Spraggon,L.,
            Squares,S.L., Steward,C.A., Sycamore,N., Tamlyn-Hall,G., Tester,J.,
            Theaker,A.J., Thomas,D.W., Thorpe,A., Tracey,A., Tromans,A.,
            Tubby,B., Wall,M., Wallis,J.M., West,A.P., White,S.S.,
            Whitehead,S.L., Whittaker,H., Wild,A., Willey,D.J., Wilmer,T.E.,
            Wood,J.M., Wray,P.W., Wyatt,J.C., Young,L., Younger,R.M.,
            Bentley,D.R., Coulson,A., Durbin,R., Hubbard,T., Sulston,J.E.,
            Dunham,I., Rogers,J. and Beck,S.
  TITLE     The DNA sequence and analysis of human chromosome 6
  JOURNAL   Nature 425 (6960), 805-811 (2003)
   PUBMED   14574404
REFERENCE   9  (bases 1 to 7494)
  AUTHORS   Bae,J., Leo,C.P., Hsu,S.Y. and Hsueh,A.J.
  TITLE     MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member
            MCL-1, encodes a proapoptotic protein possessing only the BH3
            domain
  JOURNAL   J Biol Chem 275 (33), 25255-25261 (2000)
   PUBMED   10837489
REFERENCE   10 (bases 1 to 7494)
  AUTHORS   Kasof,G.M., Goyal,L. and White,E.
  TITLE     Btf, a novel death-promoting transcriptional repressor that
            interacts with Bcl-2-related proteins
  JOURNAL   Mol Cell Biol 19 (6), 4390-4404 (1999)
   PUBMED   10330179
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D79986.1, AL121713.24 and
            BX107174.1.
            
            On May 17, 2019 this sequence version replaced NM_014739.2.
            
            Summary: This gene encodes a transcriptional repressor that
            interacts with several members of the BCL2 family of proteins.
            Overexpression of this protein induces apoptosis, which can be
            suppressed by co-expression of BCL2 proteins. The protein localizes
            to dot-like structures throughout the nucleus, and redistributes to
            a zone near the nuclear envelope in cells undergoing apoptosis.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.249788.1,
                                           SRR1803616.190790.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000531224.6/ ENSP00000435210.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5533              D79986.1           6-5538
            5534-6794           AL121713.24        14525-15785         c
            6795-7258           BX107174.1         15-478              c
            7259-7494           AL121713.24        13825-14060         c
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q23.3"
     gene            1..7494
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="BCL2 associated transcription factor 1"
                     /db_xref="GeneID:9774"
                     /db_xref="HGNC:HGNC:16863"
                     /db_xref="MIM:612588"
     exon            1..134
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            135..238
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            239..352
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..3011
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="isoform 1 is encoded by transcript variant 1;
                     bcl-2-associated transcription factor 1; BCLAF1 and THRAP3
                     family member 1"
                     /codon_start=1
                     /product="bcl-2-associated transcription factor 1 isoform
                     1"
                     /protein_id="NP_055554.1"
                     /db_xref="CCDS:CCDS5177.1"
                     /db_xref="GeneID:9774"
                     /db_xref="HGNC:HGNC:16863"
                     /db_xref="MIM:612588"
                     /translation="MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYS
                     RSRSRDRMYSRDYRRDYRNNRGMRRPYGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHS
                     RSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPRSSSSRSSSPYSKSPVSKRRG
                     SQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSSATSGDIWPGLSA
                     YDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGS
                     VGNGSSRYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRGRSSFYPDGGDQETAKTG
                     KFLKRFTDEESRVFLLDRGNTRDKEASKEKGSEKGRAEGEWEDQEALDYFSDKESGKQ
                     KFNDSEGDDTEETEDYRQFRKSVLADQGKSFATASHRNTEEEGLKYKSKVSLKGNRES
                     DGFREEKNYKLKETGYVVERPSTTKDKHKEEDKNSERITVKKETQSPEQVKSEKLKDL
                     FDYSPPLHKNLDAREKSTFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSNRPASLT
                     KDRLLASTLVHSVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSA
                     AMTLNERFTSYQKATEEHSTRQKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKG
                     DKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDS
                     KHKREQDHSRSSSSSASPSSPSSREEKESKKEREEEFKTHHEMKEYSGFAGVSRPRGT
                     FFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYTPKSKKYFLHDDRDDGV
                     DYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDEEETMENNEEKKDR
                     RKEEKE"
     exon            353..1264
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1930
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2100
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2206
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2207..2291
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2467
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2468..2645
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2646..2792
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            2793..3005
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     exon            3006..7494
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5514..5519
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="hexamer: AATAAA"
     polyA_site      5535
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="major polyA site"
     regulatory      5805..5810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="hexamer: AATAAA"
     polyA_site      5827
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
     regulatory      6195..6200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="hexamer: TATAAA"
     polyA_site      6218
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
     regulatory      7236..7241
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
                     /note="hexamer: AATAAA"
     polyA_site      7260
                     /gene="BCLAF1"
                     /gene_synonym="bK211L9.1; BTF"
ORIGIN      
        1 agatcggaag tgacggagac gtgctagcgc gtcgaaggta gctctatggt tttcctcgcg
       61 ttcttgagtc gggaaatggc cgctgtgtgg ttgcaacgga gataaattcc cggaaccgcg
      121 attcggcgtg tcaggaattc gaatttagag tttaatttct cagagcattc tctccaggaa
      181 gaatttttac agtatctcaa agacttcact tgacttcttg atcctgcata aaaccaagga
      241 gaaaagaaat gggtcgctcc aattctagat cacattcttc aaggtcaaag tctagatcac
      301 agtctagttc tcgatcaaga tcaagatctc attctagaaa gaagcgatac agttctaggt
      361 ctcgttccag aacatattca aggtctcgta gtagagatcg tatgtattct agagattatc
      421 gtcgcgatta cagaaataat agaggaatga gacgacctta tgggtacaga ggaaggggta
      481 gagggtatta tcaaggagga ggaggtagat atcatcgagg tggttataga cctgtctgga
      541 atagaaggca ctctaggagt cctagacgag gtcgttcacg ttccaggagt ccaaaaagaa
      601 gatccgtttc ttctcaaaga tccagaagca gatctcgccg gtcatataga tcttctaggt
      661 ctccaagatc atcctcttct cgttcttcat ccccatatag caaatctcct gtttctaaaa
      721 gacgagggtc tcaggaaaaa caaaccaaaa aagctgaagg ggaaccccaa gaagagagtc
      781 cgttgaaaag taaatcacag gaggaaccga aagatacatt tgaacatgac ccatctgagt
      841 ctatcgatga atttaataag tcatcagcca catccggtga tatttggcct ggcctttcag
      901 cttatgataa tagtcctaga tcaccccata gtccttcacc tattgctaca ccacctagtc
      961 agagttcatc ttgctctgat gctcccatgc tcagtacagt tcactctgca aaaaatactc
     1021 cttctcagca ttcacattcc attcagcata gtcctgaaag gtctgggtct ggttctgttg
     1081 gaaatggatc tagtcgatac agtccttctc agaatagtcc aattcatcac atcccttcac
     1141 gaagaagtcc tgcaaagaca atcgcaccac agaatgctcc aagagatgag tctaggggcc
     1201 gttcctcgtt ttatcctgat ggtggagatc aggaaactgc aaagactggg aagttcttaa
     1261 aaaggttcac agatgaagag tctagagtat tcctgcttga taggggtaat accagggata
     1321 aagaggcttc aaaagagaaa ggatcagaga aagggagggc agagggagaa tgggaagatc
     1381 aggaagctct agattacttc agtgataaag agtctggaaa acaaaagttt aatgattcag
     1441 aaggggatga cacagaggag acagaggatt atagacagtt caggaagtca gtcctcgcag
     1501 atcagggtaa aagttttgct actgcatctc accggaatac tgaggaggaa ggactcaagt
     1561 acaagtccaa agtttcactg aaaggcaata gagaaagtga tggatttaga gaagaaaaaa
     1621 attataaact taaagagact ggatatgtag tggaaaggcc tagcactaca aaagataagc
     1681 acaaagaaga agacaaaaat tctgaaagaa taacagtaaa gaaagaaact cagtcacctg
     1741 agcaggtaaa gtctgaaaag ctcaaagacc tctttgatta cagtccccct ctacacaaga
     1801 atctggatgc acgagaaaag tctaccttca gagaggaaag cccacttagg atcaaaatga
     1861 tagcgagtga ttctcaccgt cctgaagtca aactcaaaat ggcacctgtt cctcttgatg
     1921 attctaacag acctgcttcc ttgactaaag acaggctgct tgctagtaca cttgtccatt
     1981 ctgtcaagaa ggagcaagaa ttccgatcca tctttgacca cattaagttg ccacaggcca
     2041 gcaaaagcac ttcagagtca tttattcaac acattgtgtc cttggttcat catgttaaag
     2101 agcaatactt caagtcagct gcaatgaccc taaacgagcg gttcacttcg tatcagaaag
     2161 ccactgaaga acatagtact cggcaaaaga gccctgaaat acacaggaga attgacatct
     2221 caccaagtac cctgaggaag catacccgtt tagcagggga agagagagtt tttaaagaag
     2281 aaaatcaaaa gggagataaa aaattaaggt gtgactctgc tgaccttcgg catgacattg
     2341 atcgccgtag aaaagaaaga agtaaagaac ggggagattc caagggctcc agggaatcca
     2401 gtggatcaag aaagcaggaa aaaactccaa aagattacaa ggaatacaaa tcttacaaag
     2461 atgacagtaa acataaaaga gagcaagatc attctcgatc ttcatcctct tcagcatcac
     2521 cttcttctcc cagttctcga gaagaaaagg agagtaagaa ggaaagagaa gaagaattta
     2581 aaactcacca tgaaatgaaa gaatactcag gctttgcagg agttagccga ccacgaggaa
     2641 ccttttttcg aattagaggc agaggaagag ccagaggagt ttttgctggg acaaatactg
     2701 gtccaaacaa ctcaaatact acttttcaaa agagaccgaa ggaagaggaa tgggatccag
     2761 aatatacccc aaagagcaag aagtacttct tgcatgacga cagagatgat ggtgtggatt
     2821 attgggccaa aagaggaaga ggtcgtggta cttttcaacg tggcagaggg cgctttaact
     2881 tcaaaaaatc aggtagcagt cctaaatgga ctcatgacaa ataccaaggg gatgggattg
     2941 ttgaagatga agaagagacc atggaaaata atgaagaaaa gaaggacaga cgcaaggaag
     3001 aaaaggaata ataaatatga agtaagatta caacagagca gaacttgcac ccaccatttt
     3061 ttttacctga tttttgtttt caaataagaa tgtaagcatt ttacttaaat tttactgttt
     3121 gcaagtagtc tatagaaatt ttgttttaag tcttcaaata tcttgagaaa tagtagactg
     3181 tatgttgaaa attgtactga aataaagtag aaaattgtta cgtaccatat ttgtaactat
     3241 caacttttaa aacttttaac gtttttgtta catgcattgt aattctgctt tgtctataag
     3301 atatggtcaa gtacagctct gtgaaagttc tgattctctt ccttccctgt ttgtcaatgt
     3361 tttattctga agtaaacgtt agctctacat ataaatcctg gaacagaaat tgtttataga
     3421 gactacacta attattttaa ctgtatacat ctgtttaatt tgaacacact acatcgtagg
     3481 gtgactgatt tttgaagtat accacagaca aaaagttgtt actatggtaa actaagctag
     3541 tttaacactt gagcaaatgc ttaagaagga attaaaaaaa aaaagctttg ccaatagcta
     3601 aaaagtacaa gctattaaaa atcagattga aaagttttga gaaaatgtta tttttactga
     3661 aagcaagcag tggcctataa agaacattct taggagcctt ttctatttgc gttcaaaact
     3721 gtgtgttctc tttctattcc tatttgatag tttgagtcat ggtcttagat attagctatt
     3781 tgtgagagga aactggtttg taacaatact gcaaatagaa accccatttc tactgaacat
     3841 cctagtttta aacagaagaa aaactgtaat cctggggttg gtatgtagga ggtctatcct
     3901 gcagaataag ttgatacatt agtacctgat ttcatatctt acatatttat ttgagctgaa
     3961 cattagtttg tagtgtaact attagtaaaa atagagaaac acagcatact gttcattaat
     4021 agtattttaa aaaaattgtt tttcaaatgt caccaataaa agttttggca ggaagcttgt
     4081 tgcggcattg atctaacctt tttccccccc atttcagttg cagtttttgt agaatggctt
     4141 tttctttttc ctcttaagag ttctattctt caggtagata atttttcaaa tgtgaattat
     4201 cttttgtgtc tatattgata gctcttaaag gagtgaaaat ctaaaatagt aaatttcaat
     4261 gttaagtgtc tgctttatgg gcatatataa aagtagacac atttcatttg ttaatttagt
     4321 tgtgtgtgtg tgttaaaagg agctaatgct tattctgtta atgtaaactt ttgaagatct
     4381 taagtgtatt gctctttcat cttaaacact ttcgaggatt tgcagtgcgt ctagcaccta
     4441 gattacagcc aggaacattg gttaagaact gttggaaaca aaactaaaag caaactcaac
     4501 atatgtgatg tttatggccc tcagatcctt agtattgtgt gattttcccc cgttaacatg
     4561 tctttctaaa attgtctatt aaagcagagg aaatacctgc caaaggaagt atgtattgca
     4621 ttaatcaggg cataactaat attctcctgt tcagaataat acttatttac gtgtgaaagc
     4681 aacatggatg tgattcccaa cacagaattt tcatgaccct tttattgtat acaaataaat
     4741 accataacag ttacttggtt agacatcaaa tctgtgtgca tgactatgtg cttatccact
     4801 taagacaata ggtaaaaggg gatctgagaa attatgtaat agggagtggg aataaaacta
     4861 cttaattcct gtgggcaggt tatattttaa gttcaaatgc attgctttaa cctttggtta
     4921 cttttattct gttaaacaga attgaagaaa gagtattata ccagagtgta gtaggctagg
     4981 gtgattgtaa gaactctgta atagaatgtc attgtggatg ttaccttttt cagatccaag
     5041 catataaaaa gcctgtatat tttttaaaaa cacatcttaa ctccacgctt tacgatatta
     5101 taaaagttga atggttcctc ttggtaagga tatttgctta caagtgctag gaaataactc
     5161 actgatacct gcgttaacat actttgtttt gcctagagag gggcaataaa aatgaaccaa
     5221 aggatatttc cagaaaggat taagaaagct gtttaagaag gccatgactc tttaggtgtg
     5281 tatgtgtacc tttcagcatc ctaggaattt ttatactaaa agcaaaatgt tttttccagt
     5341 tagtcttctt caaggaatta ctattgttcc ttttgtcaca ggtaaaatca gtgttgggaa
     5401 ttataatttg agaaaaatat tacccagtaa cattgaatgt agatggctaa acgattctta
     5461 ctcagtgtga tgtataatga tgcaacaggg acccttgtaa attgtcatac gccaataaaa
     5521 tgtcacaagt aataactgct gttgtttgtt tacctgtgtc tatttcacac atcttatttc
     5581 tgtggcctat tttagaatat cagcgcatct gttaggaaga ttactggtgt ggtaaggctt
     5641 gataaatgct ttataaactc cctcaaacct ttctttactg tttttttgtt ttgtcttgtt
     5701 tttgtttttg tttttgtttt tgtttttgtt tttgtttttc ccttgtctcc cctgggaaaa
     5761 tgggaaattt tacagttggt aaatctaagc caaaattatt ttgaaataaa ggaattctgg
     5821 atgtccagtt tagtcctcgt tttcttacgt taatctggga ccttatcacc cataatatgg
     5881 tgattacttc tctttctaaa aacatagtag ctagtaaata agtaaaaaga attgtctttt
     5941 cattcacttt aagtaagatg tggtataatt cttaccatgt gccatcctgt cagttttaac
     6001 aaagcatttt cacagaaatt tgtgtactaa gacaaactga cacattttga ctcatacaaa
     6061 tggcaaatta gtccttaaaa attctgtgag agaaataact ctgtgtgtac atacatatgc
     6121 atgtaaagtg ttgtgtaaga tcattggtag cttaattata ctggataatt gtaatgttat
     6181 atacaaattt cttatataaa agtatgctgc attattaaca tttgtttaac ttttcttatt
     6241 tttctttttc tgggtttcaa ctggaatccc attggtgggg atgtacctta agtttccttg
     6301 tggaataata gactttcaca tccagaaaga gaagttcaga ggcctttctc aatttacaga
     6361 gttgaagagg aaaggcaaat ggaggaataa acttcattgt cattgggatt aatttggcta
     6421 gactcaatct cttgagcctt aaaatgagaa agtaatatcc attacatgtt tagagttctc
     6481 aaatagttga tactattatt aaatgtttca aattttattg gggtagatac taaaaattag
     6541 tacactgtcc taatgaagtt taaatgaaca agttttacat ttcttaggat tcaggaatat
     6601 atggaatcta gttgtattta attgacttga gagccacaga gattgtgttg atttatgagc
     6661 tggactccta ttgttttaga aacatgcagt aatgattaac ttgtgtcaat gatggtaatg
     6721 agaccaaact tgtacattta attaagggta ccagttcttt ttatgttact agactatacc
     6781 ttggccttta aaaatgaatc tcactgatta aagagaacag gcattattag gatagcattc
     6841 caccacaact agaaacattc aaataatgtg tcttaattgt aatctgtata taggaaaatt
     6901 tttcctatgg atatttttgg tgttttacca cagtgaactg atttgtagca cttatgaagt
     6961 gcagaaggta atattcttga aaatagaaaa aggttgggtg agcaggcttt aatgcctttc
     7021 ccccaagaat atacatcgaa tttttcttaa tcttttgggg ttggccagct tccagatttc
     7081 attaataatg agctctgcct ttaataaaag tacatgatca tagctacact gtatgtttag
     7141 gtggtgtgaa atgatttata atcacagctt gaactgtgtt tgcttggtac tgtcatagtg
     7201 attacaaatt tcatggaatg cgaagagcaa caataaataa aaaataccac tcaccctcca
     7261 gaatagtgga ttatttgcta ccttgagtcc ttcactatgg aaaataattt ttcagtctca
     7321 tttgtactag aagggaaaac taaactttct gttgagttct caggatactt gtaaggtggc
     7381 cgtgaaaaag aatgttaaat gcactttgaa ttatttattt atttatttat ttatttattt
     7441 atttatttat tgagatggag tcttacccag actagagtgc agtggctcga gctc
//
