LOCUS       NM_005504               9314 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens branched chain amino acid transaminase 1 (BCAT1),
            transcript variant 1, mRNA.
ACCESSION   NM_005504
VERSION     NM_005504.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Kang,Y.J., Song,W., Lee,S.J., Choi,S.A., Chae,S., Yoon,B.R.,
            Kim,H.Y., Lee,J.H., Kim,C., Cho,J.Y., Kim,H.J. and Lee,W.W.
  TITLE     Inhibition of BCAT1-mediated cytosolic leucine metabolism regulates
            Th17 responses via the mTORC1-HIF1alpha pathway
  JOURNAL   Exp Mol Med 56 (8), 1776-1790 (2024)
   PUBMED   39085353
  REMARK    GeneRIF: Inhibition of BCAT1-mediated cytosolic leucine metabolism
            regulates Th17 responses via the mTORC1-HIF1alpha pathway.
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Wang,W., Li,Y., Tang,L., Shi,Y., Li,W., Zou,L., Zhang,L., Cheng,Y.,
            Yuan,Z., Zhu,F. and Duan,Q.
  TITLE     Cross-talk between BCKDK-mediated phosphorylation and
            STUB1-dependent ubiquitination degradation of BCAT1 promotes GBM
            progression
  JOURNAL   Cancer Lett 591, 216849 (2024)
   PUBMED   38621458
  REMARK    GeneRIF: Cross-talk between BCKDK-mediated phosphorylation and
            STUB1-dependent ubiquitination degradation of BCAT1 promotes GBM
            progression.
REFERENCE   3  (bases 1 to 9314)
  AUTHORS   Pan,J., Wang,Y., Huang,S., Mao,S., Ling,Q., Li,C., Li,F., Yu,M.,
            Huang,X., Huang,J., Lv,Y., Li,X., Ye,W., Wang,H., Wang,J. and
            Jin,J.
  TITLE     High expression of BCAT1 sensitizes AML cells to PARP inhibitor by
            suppressing DNA damage response
  JOURNAL   J Mol Med (Berl) 102 (3), 415-433 (2024)
   PUBMED   38340163
  REMARK    GeneRIF: High expression of BCAT1 sensitizes AML cells to PARP
            inhibitor by suppressing DNA damage response.
REFERENCE   4  (bases 1 to 9314)
  AUTHORS   You,H., Song,G., Xu,Z., Chen,S., Shen,W., Liu,H., Deng,B., Li,J.
            and Huang,G.
  TITLE     HuR promotes castration-resistant prostate cancer progression by
            altering ERK5 activation via posttranscriptional regulation of
            BCAT1
  JOURNAL   J Transl Med 22 (1), 178 (2024)
   PUBMED   38369471
  REMARK    GeneRIF: HuR promotes castration-resistant prostate cancer
            progression by altering ERK5 activation via posttranscriptional
            regulation of BCAT1.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9314)
  AUTHORS   Zheng,J., Liu,Y., Wang,J., Shi,J., Li,L., Jiang,X. and Tao,L.
  TITLE     Integrated single-cell and bulk characterization of branched chain
            amino acid metabolism-related key gene BCAT1 and association with
            prognosis and immunogenicity of clear cell renal cell carcinoma
  JOURNAL   Aging (Albany NY) 16 (3), 2715-2735 (2024)
   PUBMED   38309289
  REMARK    GeneRIF: Integrated single-cell and bulk characterization of
            branched chain amino acid metabolism-related key gene BCAT1 and
            association with prognosis and immunogenicity of clear cell renal
            cell carcinoma.
REFERENCE   6  (bases 1 to 9314)
  AUTHORS   Eden,A. and Benvenisty,N.
  TITLE     Involvement of branched-chain amino acid aminotransferase
            (Bcat1/Eca39) in apoptosis
  JOURNAL   FEBS Lett 457 (2), 255-261 (1999)
   PUBMED   10471790
REFERENCE   7  (bases 1 to 9314)
  AUTHORS   Ben-Yosef,T., Eden,A. and Benvenisty,N.
  TITLE     Characterization of murine BCAT genes: Bcat1, a c-Myc target, and
            its homolog, Bcat2
  JOURNAL   Mamm Genome 9 (7), 595-597 (1998)
   PUBMED   9657861
REFERENCE   8  (bases 1 to 9314)
  AUTHORS   Bledsoe,R.K., Dawson,P.A. and Hutson,S.M.
  TITLE     Cloning of the rat and human mitochondrial branched chain
            aminotransferases (BCATm)
  JOURNAL   Biochim Biophys Acta 1339 (1), 9-13 (1997)
   PUBMED   9165094
REFERENCE   9  (bases 1 to 9314)
  AUTHORS   Schuldiner,O., Eden,A., Ben-Yosef,T., Yanuka,O., Simchen,G. and
            Benvenisty,N.
  TITLE     ECA39, a conserved gene regulated by c-Myc in mice, is involved in
            G1/S cell cycle regulation in yeast
  JOURNAL   Proc Natl Acad Sci U S A 93 (14), 7143-7148 (1996)
   PUBMED   8692959
REFERENCE   10 (bases 1 to 9314)
  AUTHORS   Naylor,S.L. and Shows,T.B.
  TITLE     Branched-chain aminotransferase deficiency in Chinese hamster cells
            complemented by two independent genes on human chromosomes 12 and
            19
  JOURNAL   Somatic Cell Genet 6 (5), 641-652 (1980)
   PUBMED   6933702
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK056255.1, BX474651.1,
            AC023796.22 and BU618839.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_005504.6.
            
            Summary: This gene encodes the cytosolic form of the enzyme
            branched-chain amino acid transaminase. This enzyme catalyzes the
            reversible transamination of branched-chain alpha-keto acids to
            branched-chain L-amino acids essential for cell growth. Two
            different clinical disorders have been attributed to a defect of
            branched-chain amino acid transamination: hypervalinemia and
            hyperleucine-isoleucinemia. As there is also a gene encoding a
            mitochondrial form of this enzyme, mutations in either gene may
            contribute to these disorders. Alternatively spliced transcript
            variants have been described. [provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647122.1, SRR1660809.157590.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261192.12/ ENSP00000261192.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1117              AK056255.1         359-1475
            1118-1584           BX474651.1         122-588
            1585-9084           AC023796.22        129021-136520       c
            9085-9314           BU618839.1         19-248              c
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..9314
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /note="branched chain amino acid transaminase 1"
                     /db_xref="GeneID:586"
                     /db_xref="HGNC:HGNC:976"
                     /db_xref="MIM:113520"
     exon            1..175
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..1330
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /EC_number="2.6.1.42"
                     /note="isoform 1 is encoded by transcript variant 1;
                     branched chain aminotransferase 1, cytosolic; placental
                     protein 18; branched chain amino-acid transaminase 1,
                     cytosolic"
                     /codon_start=1
                     /product="branched-chain-amino-acid aminotransferase,
                     cytosolic isoform 1"
                     /protein_id="NP_005495.2"
                     /db_xref="CCDS:CCDS44845.1"
                     /db_xref="GeneID:586"
                     /db_xref="HGNC:HGNC:976"
                     /db_xref="MIM:113520"
                     /translation="MKDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDP
                     NNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFR
                     GVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASL
                     YIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGT
                     GDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEEL
                     ATPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLTTALEGNRVREMFGSGTA
                     CVVCPVSDILYKGETIHIPTMENGPKLASRILSKLTDIQYGREESDWTIVLS"
     exon            176..247
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            248..448
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            449..559
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            560..679
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            680..843
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            844..986
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1072
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1213
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1288
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..9314
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7956..7961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /note="hexamer: AATAAA"
     regulatory      7961..7966
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /note="hexamer: AACAAA"
     polyA_site      7975
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /note="major polyA site"
     polyA_site      7994
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
     regulatory      9294..9299
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
                     /note="hexamer: AATAAA"
     polyA_site      9314
                     /gene="BCAT1"
                     /gene_synonym="BCATC; BCT1; ECA39; MECA39; PNAS121; PP18"
ORIGIN      
        1 gcaatccagc ccgccacgtg tactcgccgc cgcctcgggc actgccccag gtcttgctgc
       61 agccgggacc gcgctctgca gccgcagacc cggtccacac ggccaggggc tacgaccctt
      121 gggatctgcc ctccgctcag ctcgagcttc cctcgtggcc gacggaacaa tgaaggattg
      181 cagtaacgga tgctccgcag agtgtaccgg agaaggagga tcaaaagagg tggtggggac
      241 ttttaaggct aaagacctaa tagtcacacc agctaccatt ttaaaggaaa aaccagaccc
      301 caataatctg gtttttggaa ctgtgttcac ggatcatatg ctgacggtgg agtggtcctc
      361 agagtttgga tgggagaaac ctcatatcaa gcctcttcag aacctgtcat tgcaccctgg
      421 ctcatcagct ttgcactatg cagtggaatt atttgaagga ttgaaggcat ttcgaggagt
      481 agataataaa attcgactgt ttcagccaaa cctcaacatg gatagaatgt atcgctctgc
      541 tgtgagggca actctgccgg tatttgacaa agaagagctc ttagagtgta ttcaacagct
      601 tgtgaaattg gatcaagaat gggtcccata ttcaacatct gctagtctgt atattcgtcc
      661 tacattcatt ggaactgagc cttctcttgg agtcaagaag cctaccaaag ccctgctctt
      721 tgtactcttg agcccagtgg gaccttattt ttcaagtgga acctttaatc cagtgtccct
      781 gtgggccaat cccaagtatg taagagcctg gaaaggtgga actggggact gcaagatggg
      841 agggaattac ggctcatctc tttttgccca atgtgaagca gtagataatg ggtgtcagca
      901 ggtcctgtgg ctctatggag aggaccatca gatcactgaa gtgggaacta tgaatctttt
      961 tctttactgg ataaatgaag atggagaaga agaactggca actcctccac tagatggcat
     1021 cattcttcca ggagtgacaa ggcggtgcat tctggacctg gcacatcagt ggggtgaatt
     1081 taaggtgtca gagagatacc tcaccatgga tgacttgaca acagccctgg aggggaacag
     1141 agtgagagag atgtttggct ctggtacagc ctgtgttgtt tgcccagttt ctgatatact
     1201 gtacaaaggc gagacaatac acattccaac tatggagaat ggtcctaagc tggcaagccg
     1261 catcttgagc aaattaactg atatccagta tggaagagaa gagagcgact ggacaattgt
     1321 gctatcctga atggaaaata gaggatacaa tggaaaatag aggataccaa ctgtatgcta
     1381 ctgggacaga ctgttgcatt tgaattgtga tagatttctt tggctacctg tgcataatgt
     1441 agtttgtagt atcaatgtgt tacaagagtg attgtttctt catgccagag aaaatgaatt
     1501 gcaatcatca aatggtgttt cataacttgg tagtagtaac ttaccttacc ttacctagaa
     1561 aaacattaat gtaagccata taacatggga ttttcctcaa tgattttagt gcctcctttt
     1621 gtacttcact cagatactaa atagtagttt attctttaat ataagttaca ttctgctcct
     1681 caaacaaatg caattttttg tgtgtgtttg aaagctaatt tgagaaaatt tcataggtta
     1741 catttcctgc agcctatctt tatccacaga aagtgttttc ttttttttaa atcaagactt
     1801 ttaaaactgg atttcctccc atcactgttt tttgaaggtc ctccaagtcc gtgttaaggt
     1861 aaatatctgt tttcttcctg atgtcacagc ctgagcatac tctgtgcatt aggaagacct
     1921 gagtgcattt cccaccattg tcctttccac attatgttgt agctggctgg ctgtcaggcg
     1981 actacaagac tgagggtctt gtgccttata gatctttgta tcccccatgg ctgacatata
     2041 gtaggtactc agtaaatggt tttataatga atcagtgaac attttgcttc tatagaagtg
     2101 taccttcttt gtttctatat tatgaaacct ctttattaga atttgtgatt gattctgaca
     2161 gtgtatagat ttaccttata ttgtctttat tttccatgag ctactaagtc attagagata
     2221 ctctgaagca tagttagttt aggaaatcac ttcatattga ttgtattaga attatcttgg
     2281 aattgaagat atatccctag agcaggggac cccaaccccc aggccatggg ccacacagca
     2341 ggaagaggtg agtggtgggc cattgaggag cttcatctgt atttatggct acttcccatc
     2401 actcgaatta ccacctgaac tccacctctt gtcagctcag tggcagcatt agattctcat
     2461 aggagcacaa atcctattgt gaactctgca tgcaagggat ctaggctatg cgctccttat
     2521 gagaatctaa tgcttgatga cctgaggtgt aacagtttca tcctgaaacc acccttcacc
     2581 ctgcagtctg tggaaaaatt gtcttccaca aaactggtcc ctggtgccaa aaatgttggg
     2641 gaccactgct ctagagagag gtcatgatat cataccaacc aaatggaaat gacaaatgtt
     2701 ttatgtcaag tgttaattgc agaaataaat cttttttttt tttttttggt agaaaacaaa
     2761 gaggcatact ctgattttta tactctgttt ttgcaggtgc tcttttcttt gaatggagat
     2821 ttgatgagca agtggttagg atgcagggag agctactatg ggtgatattt tccttgttta
     2881 ggagctgtga gttaaaattg tatcctttgt ggtttatcta aggaaagtca aatcttgaca
     2941 gaaaacattt ttccttggaa ggtcaactct cagacattgt attttggttt ccctcagtcc
     3001 tcataacttc cttcttgctg aacatatttt attctctttt cagagaagga aaataaaaag
     3061 gattctaaaa gtttgatgca ttggaaaaat ttccttgagg catttagcaa cacatagaaa
     3121 atgggctttg attcttttcc aaaactttta gccatagggt cttttataga cagggatagt
     3181 aaaatgaaaa ttgagaaata taagatgaaa aggaatgata aaaatatctt ttagggggct
     3241 tttaattggt gatctgaaat cttgggagaa gctgttcttt tcaggcctga ggtgctcttg
     3301 actgtcgcct gcgcactgtg taccccgagc aacattctaa gggtgtgctt tcgccttggc
     3361 taactccttt gacctcattc ttcatatagt agtctaggaa aaagttgcag gtaatttaaa
     3421 ctgtctagtg gtacatagta actaaatttc tattcctatg agaaatgaga attatttatt
     3481 tgccatcaac acattttata ctttgcatct ccaaatttat tgtggcgaga cttgtccatt
     3541 gtgaaagtta gagaacatta tgtttgtatc atttctttca taaaacctca agagcatttt
     3601 taagcccttt tcatcagacc cagtgaaaac taaggataga tgtttaaaaa ctggaggtct
     3661 cctgataagg agaacacaat ccaccattgt catttaagta ataagacagg aaattgacct
     3721 tgacgctttc ttgttaaata gatttaacag gaacatctgc acatcttttt tccttgtgca
     3781 ctatttgttt aattgcagtg gattaataca gcaagagtgc cacattataa ctaggcaatt
     3841 atccattctt caagacttag ttattgtcac actaattgat cgtttaaggc ataagatggt
     3901 ctagcattag gaacatgtga agctaatctg ctcaaaaaga tcaacaaatt aatattgttg
     3961 ctgatatttg cataattggc tgcaattatt taatgtttaa ttgggttgat caaatgagat
     4021 tcagcaattc acaagtgcat taatataaac agaactggtg gcacttaaaa tgataatgat
     4081 taacttatat tgcatgttct cttcctttca cttttttcag tttctacatt tcagaccgag
     4141 cttgtcagct tttttgaaaa cacatcagta gaaaccaaga ttttaaaatg aagtgtcaag
     4201 acaaaggcaa aacctgagca gttcctaaaa agatttgctg ttagaaattt tctttgtggc
     4261 agtcatttat taaggattca actcgtgata caccaaaaga agagttgact tcagagatgt
     4321 gttccatgct ctctagcaca ggaatgaata aatttataac acctgcttta gcctttgttt
     4381 tcaaaagcac aaaggaaaag tgaaagggaa agagaaacaa gtgactgaga agtcttgtta
     4441 aggaatcagg ttttttctac ctggtaaaca ttctctattc ttttctcaaa agattgctgt
     4501 aagaaaaaat gtaagacaaa aaaaaaaaaa aaaaacaaac agaggcagag gcaggcagta
     4561 gcaagaaagc agagcgtaac atcagctaga tggtaacatg caatgtcagc tctcttgaag
     4621 acatgggaaa cctaagttac accttgggtt aaaattcttc accatattag ttttgttgct
     4681 tcataaaatt tacctaagca agtggtcttg cttgcctcaa atccaagcag tcttgaacac
     4741 ttggaggcaa ttaatgagta tatcttagtc aaaagaattg ttggagcttt ttattaaagc
     4801 tacagtttca gttctgcttt tggggaattg tgctatgaaa gcagctgcca aaataagctc
     4861 atttattttc ttcaatccca ctcagtgctc agtcactata ttctgtttcc tttttttttt
     4921 tcaagttgca tatttggttt ccccttatga ttgggaaaga tgaattttca gcagaaaaca
     4981 ttgtttgttc actttcaaag agtgatagtt tctaaaacat ttagagcaat aaatattcat
     5041 cagaggtacc aagtaagccg gcagaagagt taagggttag agaaatccct tatttcatgt
     5101 cttgactcta aaattatcaa agtacttttc cttgtaatgt ggatttcttc ttatgcggat
     5161 atgcaaaaac ttcagttata cgtagtaatg ctagcaggta attttagtag acattttata
     5221 acaactgtca ctttgtttcg ccacatgtag agtttgttca gctattttcc agatatctcc
     5281 ccacaaaagg aggcaaaggg taccagcttt tcaatgagca ttacctatta cttggcaaag
     5341 atgatgaaga ctctattaat agttcatttg ataaatgttg acataaccaa caatagagat
     5401 taggaagtta gttttaagaa atcaatggca tatagacatt accctcatgg agtttgtatt
     5461 ctactacttg aactgattgt agctataaaa gcatagttag atagctgaat agttagatca
     5521 taagcaaaga aggccagaac acatctctta tcaagaaatc aatgaatagt ttatctcatt
     5581 tttaaagcaa ctttatcctt ctttaattcc ttcctttctt ctagtgcaaa actacttaat
     5641 aaggttggtg tttaggttag tgttcacacc attcctcatc tggtgtgaat taccttctct
     5701 ttctttacta tttactacca acctagtaca tgtgttgact gaattctttt caaacaatgt
     5761 tgagttatca tggtgcacct aataaattaa caccacagat tacagcatcc ttgctgattt
     5821 tctcagcaaa gccagattag atggaaataa acaaagaaaa tgatcctaga gtgaattttt
     5881 ctagaaaata tctattatga accatgctgt ttaaagtatt agcttgaagg tgatggatcc
     5941 agctattcag aaaataactt tcatataacc atgattttgc acagtatgag gtcttaaatg
     6001 tgtggaaaga gataaatttt ttatcattac cacaaacccc ttttaaagat tcaaaggtgg
     6061 aagaaagtga tttatttttt ctcttcagca tacatatata aaagacttgt cagatgttta
     6121 atttggggag gttgataatg aaacatatca acagagtata gtagttatag tagtgtttgt
     6181 gggtaaataa tttcctgggg tcagacatat ataaacatat ttgcttcaaa atgataaagg
     6241 catgaaatca gtcttaaaaa ttgaaatggg ggtgatgggg gagaaaaaga agaacaaatt
     6301 tgaagtgccc tttcaaatct gctggataca agtattgaag ttttaagtca tcttattctg
     6361 tctgaaagtg tatttttcat tctacaatag acccaatcaa caagacgtat aacttgagtt
     6421 gcatgatgtt cagtttatgt aatctactgt tgggatggta agaattgatg taggctgtgg
     6481 tgtaagaatg aattaaaata tagtttcact ggcttttctc tacatatcca ctatcacaat
     6541 ggctaggttt cctgttgctc actattggat tctggagaaa aatttaatga aagatgatat
     6601 cagaggaaga ataagtggag gtagagaaga aaggaatgat agaggagggg aaaaaaacaa
     6661 aacatatttt tgtgttatcc aaaggagctt tttccttatt ctgtcaagca ttgagatctt
     6721 cttcagcttt caatgtagtt gctaaataca aataatgcta ctaggtagtg actaaatata
     6781 gcaaacactt catcagatat tagaattagg tcacactatt gaggttataa tctgaaggtt
     6841 gtgttacata gaaaccactt tagattatta tcaacttgga ctaggcttta ttttataata
     6901 gcatagtaag taatatctat tgtgtcattt cttcaaccat tttattctaa gatccatgaa
     6961 gcttcttgag gccaaataaa ataataagtt tagacaagaa gtagattgtg acttttttcc
     7021 cttagagata ctatttacta tctcctatcc tgataggtgg aaggtttact gaattggaaa
     7081 ttggttgact attagttttt aactaaaatg tgcaataaca cattgcagtt tcctcaaact
     7141 agtttcctat gatcattaaa ctcattctca gggttaagaa aggaatgtaa atttctgcct
     7201 caatttgtac ttcatcaata agtttttgaa gagtgcagat ttttagtcag gtcttaaaaa
     7261 taaactcaca aatctggatg catttctaaa ttctgcaaat gtttcctggg gtgacttaac
     7321 aaggaataat cccacaatat acctagctac ctaatacatg gagctggggc tcaacccact
     7381 gtttttaagg atttgcgctt acttgtggct gaggaaaaat aagtagttcg aggaagtagt
     7441 ttttaaatgt gagcttatag atagaaacag aatatcaact taattatgaa attgttagaa
     7501 cctgttctct tgtatctgaa tctgattgca attactattg tactgataga ctccagccat
     7561 tgcaagtctc agatatctta gctgtgtagt gattcttgaa attcttttta agaaaaattg
     7621 agtagaaaga aataaaccct ttgtaaatga ggcttggctt ttgtgaaaga tcatccgcag
     7681 gctatgttaa aaggatttta gctcactaaa agtgtaataa tggaaatgtg gaaaatatcg
     7741 taggtaaagg aaactacctc atgctctgaa ggttttgtag aagcacaatt aaacatctaa
     7801 aatggctttg ttacaccaga gccatctggt gtgaagaact ctatatttgt atgttgagag
     7861 ggcatggaat aattgtattt tgctggcaat agacacattc tttattattt gcagattcct
     7921 catcaaatct gtaattatgc acagtttctg ttatcaataa aacaaaagaa tcctgtttgt
     7981 gtggtttcat gaaatcagca ttgttgaatg catgaagtaa taatgctaaa ttaacatttt
     8041 tatgatgtct caaggtttct ggtcaaggga agtaaatgta ggatagtatt tttacaccaa
     8101 aatgacacag agagaattga gcacaccaga aagaccagaa accacaccac tggatagaga
     8161 ttcaatatgt ttctttttca aacatttgga caagaaaaaa atgggcattt aaaaattctt
     8221 ccttcccctg gttatggatt tatctgtagt aaaacttagc tttgtcgttt gagatttgca
     8281 cagaatgggg ggagtagatt acctcttccc attcattgtc ataatggatc tacatcactg
     8341 ataaacacca tacttctata tgtggttaac tagctttaga ataaaagaca ctttaaaaag
     8401 taaaaggcct agagatcttc aatgaagtgt cccttttagc caaaccaggc cttgcagaaa
     8461 ttgtcctcaa aagcaccaag ggagaacaaa gccaagtgca gaattaccca agggtcacac
     8521 attttgtatt cattttctta taatttgccc aaatgatttg aagtacagca aaacctgaag
     8581 ttttgcaaag agttactgta aactgaactt aaaaatgtca gagtgctcgg tgacccactt
     8641 ctccagaccc tgtcaacctg tgaaaatatt ggcctttatt cagatctctc aagaagttac
     8701 gcgcagagtt tggaaggtct aggcaaaggt tattagtcaa gtgttcttac agtgtcaacg
     8761 ctcaattccc acaagcgtga gaaagagaga cctgtcattc ctgagggtga tgacatacat
     8821 ttactggagc ttatataatt tatcagataa gacagcagtt tccttcaggg tagaaagtgt
     8881 gttttctaca ttgatttagt acaaaacaaa aagaaaaggg gatatttcaa attttataat
     8941 tatttttctg ctaagctgat tcagtgtgat ttaagcatat ttttcaaatc atgaatctga
     9001 ttccatatac atatgtgcct tatattgtga taatttattt ttaagtgaaa tatgctatca
     9061 tagcctgact ttatgtatag tggtgacaac ttgcaggatc gcatttctgt aaccaaacgg
     9121 ccgacagctg aggtgtagat gcttcctatg gtctgtagaa taatcactgg gcttgttctc
     9181 tagctatgtc tgtatgcaat cgcgacagtg ttgatcaaaa cccatgatca ttctctccaa
     9241 aggtctttgt cactaagcca ctagggattc tgaaaagttc gtgagctgaa acaaataaat
     9301 tgagttggaa gatt
//
