LOCUS       NM_004323               3820 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens BAG cochaperone 1 (BAG1), transcript variant 1, mRNA.
ACCESSION   NM_004323
VERSION     NM_004323.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Kizilboga,T., Ozden,C., Can,N.D., Onay Ucar,E. and Dinler
            Doganay,G.
  TITLE     Bag-1-mediated HSF1 phosphorylation regulates expression of heat
            shock proteins in breast cancer cells
  JOURNAL   FEBS Open Bio 14 (9), 1559-1569 (2024)
   PUBMED   39049197
  REMARK    GeneRIF: Bag-1-mediated HSF1 phosphorylation regulates expression
            of heat shock proteins in breast cancer cells.
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Kilbas,P.O., Can,N.D., Kizilboga,T., Ezberci,F., Doganay,H.L.,
            Arisan,E.D. and Dinler Doganay,G.
  TITLE     CRISPR/Cas9-mediated Bag-1 knockout increased mesenchymal
            characteristics of MCF-7 cells via Akt hyperactivation-mediated
            actin cytoskeleton remodeling
  JOURNAL   PLoS One 17 (1), e0261062 (2022)
   PUBMED   34995286
  REMARK    GeneRIF: CRISPR/Cas9-mediated Bag-1 knockout increased mesenchymal
            characteristics of MCF-7 cells via Akt hyperactivation-mediated
            actin cytoskeleton remodeling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3820)
  AUTHORS   Lv,J.L., Shi,L.N., Zhai,C.Y., Wang,G.J. and Qu,Y.
  TITLE     Bag-1L Protects against Cell Apoptosis in an In Vitro Model of Lung
            Ischemia-Reperfusion Injury through the C-Terminal 'Bag' Domain
  JOURNAL   Biomed Res Int 2021, 8822807 (2021)
   PUBMED   34056003
  REMARK    GeneRIF: Bag-1L Protects against Cell Apoptosis in an In Vitro
            Model of Lung Ischemia-Reperfusion Injury through the C-Terminal
            ''Bag'' Domain.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3820)
  AUTHORS   Liu,H.Y., Wang,Z.M., Bai,Y., Wang,M., Li,Y., Wei,S., Zhou,Q.H. and
            Chen,J.
  TITLE     Different BAG-1 isoforms have distinct functions in modulating
            chemotherapeutic-induced apoptosis in breast cancer cells
  JOURNAL   Acta Pharmacol Sin 30 (2), 235-241 (2009)
   PUBMED   19151744
  REMARK    GeneRIF: Distinct isoforms of BAG-1 have different anti-apoptotic
            functions in breast cancer cells, and that the BAG-1 p50 isoform
            can potentiate the role of estrogen in ER-positive breast cancer.
REFERENCE   5  (bases 1 to 3820)
  AUTHORS   Lee,S.S., Crabb,S.J., Janghra,N., Carlberg,C., Williams,A.C.,
            Cutress,R.I., Packham,G. and Hague,A.
  TITLE     Subcellular localisation of BAG-1 and its regulation of vitamin D
            receptor-mediated transactivation and involucrin expression in oral
            keratinocytes: implications for oral carcinogenesis
  JOURNAL   Exp Cell Res 313 (15), 3222-3238 (2007)
   PUBMED   17662274
  REMARK    GeneRIF: BAG-1L endogenously regulates gene expression by
            potentiating nuclear hormone receptor function and a role for
            BAG-1L in 24-hydroxylase regulation of vitamin D metabolism and the
            cellular response of oral keratinocytes to
            1alpha,25-dihydroxyvitamin D3.
REFERENCE   6  (bases 1 to 3820)
  AUTHORS   Yang,X., Chernenko,G., Hao,Y., Ding,Z., Pater,M.M., Pater,A. and
            Tang,S.C.
  TITLE     Human BAG-1/RAP46 protein is generated as four isoforms by
            alternative translation initiation and overexpressed in cancer
            cells
  JOURNAL   Oncogene 17 (8), 981-989 (1998)
   PUBMED   9747877
REFERENCE   7  (bases 1 to 3820)
  AUTHORS   Takayama,S., Krajewski,S., Krajewska,M., Kitada,S., Zapata,J.M.,
            Kochel,K., Knee,D., Scudiero,D., Tudor,G., Miller,G.J.,
            Miyashita,T., Yamada,M. and Reed,J.C.
  TITLE     Expression and location of Hsp70/Hsc-binding anti-apoptotic protein
            BAG-1 and its variants in normal tissues and tumor cell lines
  JOURNAL   Cancer Res 58 (14), 3116-3131 (1998)
   PUBMED   9679980
  REMARK    GeneRIF: Alternate protein isoforms arise through the use of a
            non-AUG (CUG) codon in addition to downstream in-frame AUG codons.
REFERENCE   8  (bases 1 to 3820)
  AUTHORS   Packham,G., Brimmell,M. and Cleveland,J.L.
  TITLE     Mammalian cells express two differently localized Bag-1 isoforms
            generated by alternative translation initiation
  JOURNAL   Biochem J 328 (Pt 3) (Pt 3), 807-813 (1997)
   PUBMED   9396724
  REMARK    GeneRIF: The longer p50 isoform is generated through the use of an
            upstream, in-frame, non-AUG (CUG) translation initiation codon.
REFERENCE   9  (bases 1 to 3820)
  AUTHORS   Zeiner,M. and Gehring,U.
  TITLE     A protein that interacts with members of the nuclear hormone
            receptor family: identification and cDNA cloning
  JOURNAL   Proc Natl Acad Sci U S A 92 (25), 11465-11469 (1995)
   PUBMED   8524784
REFERENCE   10 (bases 1 to 3820)
  AUTHORS   Takayama,S., Sato,T., Krajewski,S., Kochel,K., Irie,S., Millan,J.A.
            and Reed,J.C.
  TITLE     Cloning and functional analysis of BAG-1: a novel Bcl-2-binding
            protein with anti-cell death activity
  JOURNAL   Cell 80 (2), 279-284 (1995)
   PUBMED   7834747
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356472.17 and AL161445.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004323.5.
            
            Summary: The oncogene BCL2 is a membrane protein that blocks a step
            in a pathway leading to apoptosis or programmed cell death. The
            protein encoded by this gene binds to BCL2 and is referred to as
            BCL2-associated athanogene. It enhances the anti-apoptotic effects
            of BCL2 and represents a link between growth factor receptors and
            anti-apoptotic mechanisms. Multiple protein isoforms are encoded by
            this mRNA through the use of a non-AUG (CUG) initiation codon, and
            three alternative downstream AUG initiation codons. A related
            pseudogene has been defined on chromosome X. [provided by RefSeq,
            Feb 2010].
            
            Transcript Variant: This transcript (1) encodes multiple isoforms
            due to the use of alternative translation initiation codons. The
            longest isoform (BAG-1L or p50) is derived from an upstream non-AUG
            (CUG) start codon, while three shorter isoforms are derived from
            downstream AUG start codons. The longest isoform (BAG-1L) is
            represented in this RefSeq.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: This CCDS ID represents the longest human BAG1 isoform,
            known as BAG-1L or p50, as described in the literature, including
            PMIDs 9396724, 9679980, 9747877 and 17662274. This isoform
            initiates translation at a non-AUG (CUG) start codon that is
            well-conserved and present in a strong Kozak signal context.
            Alternative translation initiation at downstream AUG start codons
            produces three additional isoforms with shorter N-termini, known as
            BAG-1M or p46, BAG-1S or p36 (also known as p33), and p29. The most
            abundant of the shorter isoforms, BAG-1S, is represented by CCDS
            55301.1. Evidence in PMIDs 9747877 and 17662274 indicates that
            these isoforms have distinct subcellular distributions, which may
            contribute to the multifunctionality of the protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK222749.1, SRR3476690.264741.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2147975, SAMEA2149876
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match       :: ENST00000634734.3/ ENSP00000489189.2
            non-AUG initiation codon :: PMID: 9679980, 9396724
            RefSeq Select criteria   :: based on conservation, expression,
                                        longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-485               AL356472.17        6470-6954           c
            486-614             AL356472.17        4948-5076           c
            615-697             AL356472.17        3333-3415           c
            698-811             AL356472.17        1166-1279           c
            812-919             AL161445.10        160447-160554       c
            920-982             AL161445.10        159511-159573       c
            983-3820            AL161445.10        156117-158954       c
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.3"
     gene            1..3820
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /note="BAG cochaperone 1"
                     /db_xref="GeneID:573"
                     /db_xref="HGNC:HGNC:937"
                     /db_xref="MIM:601497"
     exon            1..485
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1072
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /note="isoform BAG-1L is encoded by transcript variant 1;
                     non-AUG (CUG) translation initiation codon;
                     receptor-associated protein, 46-KD; Bcl-2-binding protein;
                     Bcl-2 associating athanogene-1 protein; glucocortoid
                     receptor-associated protein RAP46; BAG family molecular
                     chaperone regulator 1; BCL2-associated athanogene; BCL2
                     associated athanogene 1"
                     /codon_start=1
                     /product="BAG family molecular chaperone regulator 1
                     isoform BAG-1L"
                     /protein_id="NP_004314.6"
                     /db_xref="CCDS:CCDS35004.1"
                     /db_xref="GeneID:573"
                     /db_xref="HGNC:HGNC:937"
                     /db_xref="MIM:601497"
                     /translation="MAQRGGARRPRGDRERLGSRLRALRPGREPRQSEPPAQRGPPPS
                     GRPPARSTASGHDRPTRGAAAGARRPRMKKKTRRRSTRSEELTRSEELTLSEEATWSE
                     EATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVT
                     SQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLI
                     GKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDR
                     RVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETE
                     RLQSTNFALAE"
     exon            486..614
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     exon            615..697
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     exon            698..811
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     exon            812..919
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     exon            920..982
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     exon            983..3820
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1243..1248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /note="hexamer: AGTAAA"
     polyA_site      1266
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /note="major polyA site"
     regulatory      3800..3805
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
                     /note="hexamer: AATAAA"
     polyA_site      3820
                     /gene="BAG1"
                     /gene_synonym="BAG-1; HAP; RAP46"
ORIGIN      
        1 agcttccatc gctgggcggt caacaagtgc gggcctggct cagcgcgggg gggcgcggag
       61 accgcgaggc gaccgggagc ggctgggttc ccggctgcgc gcccttcggc caggccggga
      121 gccgcgccag tcggagcccc cggcccagcg tggtccgcct ccctctgggc gtccacctgc
      181 ccggagtact gccagcgggc atgaccgacc caccaggggc gccgccgccg gcgctcgcag
      241 gccgcggatg aagaagaaaa cccggcgccg ctcgacccgg agcgaggagt tgacccggag
      301 cgaggagttg accctgagtg aggaagcgac ctggagtgaa gaggcgaccc agagtgagga
      361 ggcgacccag ggcgaagaga tgaatcggag ccaggaggtg acccgggacg aggagtcgac
      421 ccggagcgag gaggtgacca gggaggaaat ggcggcagct gggctcaccg tgactgtcac
      481 ccacagcaat gagaagcacg accttcatgt tacctcccag cagggcagca gtgaaccagt
      541 tgtccaagac ctggcccagg ttgttgaaga ggtcataggg gttccacagt cttttcagaa
      601 actcatattt aagggaaaat ctctgaagga aatggaaaca ccgttgtcag cacttggaat
      661 acaagatggt tgccgggtca tgttaattgg gaaaaagaac agtccacagg aagaggttga
      721 actaaagaag ttgaaacatt tggagaagtc tgtggagaag atagctgacc agctggaaga
      781 gttgaataaa gagcttactg gaatccagca gggttttctg cccaaggatt tgcaagctga
      841 agctctctgc aaacttgata ggagagtaaa agccacaata gagcagttta tgaagatctt
      901 ggaggagatt gacacactga tcctgccaga aaatttcaaa gacagtagat tgaaaaggaa
      961 aggcttggta aaaaaggttc aggcattcct agccgagtgt gacacagtgg agcagaacat
     1021 ctgccaggag actgagcggc tgcagtctac aaactttgcc ctggccgagt gaggtgtagc
     1081 agaaaaaggc tgtgctgccc tgaagaatgg cgccaccagc tctgccgtct ctggagcgga
     1141 atttacctga tttcttcagg gctgctgggg gcaactggcc atttgccaat tttcctactc
     1201 tcacactggt tctcaatgaa aaatagtgtc tttgtgattt tgagtaaagc tcctatctgt
     1261 tttctccttc tgtctctgtg gttgtactgt ccagcaatcc accttttctg gagagggcca
     1321 cctctgccca aattttccca gctgtttgga cctctgggtg ctttctttgg gctggtgaga
     1381 gctctaattt gccttgggcc agtttcaggt ttataggccc cctcagtctt cagatacatg
     1441 agggcttctt tgctcttgtg atcgtgtagt cccatagctg taaaaccaga atcaccagga
     1501 ggttgcacct agtcaggaat attgggaatg gcctagaaca aggtgtttgg cacataagta
     1561 gaccacttat ccctcattgt gacctaattc cagagcatct ggctgggttg ttgggttcta
     1621 gactttgtcc tcacctccca gtgaccctga ctagccacag gccatgagat accagggggc
     1681 cgttccttgg atggagcctg tggttgatgc aaggcttcct tgtccccaag caagtcttca
     1741 gaaggttaga acccagtgtt gactgagtct gtgcttgaaa ccaggccaga gccatggatt
     1801 aggaagggca aagagaaggc accagaatga gtaaagcagg caggtggtga agccaaccat
     1861 aaacttctca ggagtgacat gtgcttcctt caaaggcatt tttgttaacc atatccttct
     1921 gagttctatg tttccttcac agctgttcta tccattttgt ggactgtccc ccacccccac
     1981 cccatcattg tttttaaaaa attaaggcct ggcgcagcag ctcatgccta taatcccagc
     2041 actttgggag gctgaggcgg gcggatcact tgaggccagg agtttgagac cagcccaggc
     2101 aacatagcaa aaccccattc tgctttaaaa aaaaaaaaaa aaaaaattag cttggcgtag
     2161 tggcatgtgc ctataatccc agctactggg gaggctgagg cacaagaatc atttgaacct
     2221 gggaggtaga ggttgctgtg agccgagatt acgcccctgc actccagcct gggtcacaga
     2281 gtgagactcc atctcagaaa aaaaaaaaat tgagtcaggt gcagtagctc cttcctgtag
     2341 tcccagctac ttgggaggct gaggctagag gatcacttga gcccaggagt ttgagtctag
     2401 tctgggcaac atagcaagac cccatctcta aaatttaagt aagtaaaagt agataaataa
     2461 aaagaaaaaa aaactgttta tgtgctcatc ataaagtaga agagtggttt gctttttttt
     2521 ttttttttgg attaatgagg aaatcattct gtggctctag tcataattta tgcttaataa
     2581 cattgatagt agccctttgc gctataactc tacctaaaga ctcacatcat ttggcagaga
     2641 gagagtcgtt gaagtcccag gaattcagga ctgggcaggt taagacctca gacaaggtag
     2701 tagaggtaga cttgtggaca aggctcgggt cccagcccac cgcaccccaa ctttaatcag
     2761 agtggttcac tattgatcta tttttgtgtg atagctgtgt ggcgtgggcc acaacattta
     2821 atgagaagtt actgtgcacc aaactgccga acaccattct aaactattca tatatattag
     2881 tcatttaatt cttacataac ttgagaggta gacagatatc cttattttag agatgaggaa
     2941 accaagagaa cttaggtcat tagcgcaagg ttgtagagta agcggcaaag ccaagacaca
     3001 aagctgggtg gtttggtttc agagccagtg cttttcccct ctactgtact gcctctcaac
     3061 caacacaggg ttgcacaggc ccattctctg atttttttcc tcttgtcctc tgcctctccc
     3121 tctagctccc acttcctctc tgctctagtt cattttcttt agagcagccc gagtgatcat
     3181 gaagtgcaaa tcttgccatg tcagtcccct gcttagaacc ctccaatggc tcactttctc
     3241 tttaggcaaa agtctttacc ccatgccttc tcccatctca tctcaacccc ctcatttgtt
     3301 ggctgtctgc tgtcagccac tcttctttca ggtcctcaga tgcactgcac cctctcctgc
     3361 ctgggggtct ttgctcctgc tactacctct gcttgaacag ctcctcacct tccttcctcc
     3421 aaccctaccc ttgtataggt gacttttgtt catccttcag aattcaactc acatgtctct
     3481 tgcatggaga accctcacct actgtgttga gaccctgtcc agcccccagg tgggatcctc
     3541 tctcgacttc ccatacattt ctttcacagc atttacatag tccatgatag tttacttgtg
     3601 ggattatttg gttaatcttt gcctttaaca ccagggttcc ttgggtgaag gagcttcttt
     3661 atcttggtaa cagcattatt tcaagcataa cttgtaatat agttatatta catatataac
     3721 atatatatat ataacataac atatataaca tatataacaa gcataacttg ttatatagtc
     3781 ttgtatatag taagacctca ataaatattt ggagaacaaa
//
