LOCUS       NM_032989                929 bp    mRNA    linear   PRI 15-MAR-2025
DEFINITION  Homo sapiens BCL2 associated agonist of cell death (BAD),
            transcript variant 2, mRNA.
ACCESSION   NM_032989
VERSION     NM_032989.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Wang,Y., Dong,Y., Zhang,W., Wang,Y., Jao,Y., Liu,J., Zhang,M. and
            He,H.
  TITLE     AMPK/mTOR/p70S6K axis prevents apoptosis of Porphyromonas
            gingivalis-infected gingival epithelial cells via BadSer136
            phosphorylation
  JOURNAL   Apoptosis 28 (7-8), 1012-1023 (2023)
   PUBMED   37014579
  REMARK    GeneRIF: AMPK/mTOR/p70S6K axis prevents apoptosis of Porphyromonas
            gingivalis-infected gingival epithelial cells via Bad[Ser136]
            phosphorylation.
REFERENCE   2  (bases 1 to 929)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 929)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   4  (bases 1 to 929)
  AUTHORS   Kumari,A., Gesumaria,L., Liu,Y.J., Hughitt,V.K., Zhang,X.,
            Ceribelli,M., Wilson,K.M., Klumpp-Thomas,C., Chen,L., McKnight,C.,
            Itkin,Z., Thomas,C.J., Mock,B.A., Schrump,D.S. and Chen,H.
  TITLE     mTOR inhibition overcomes RSK3-mediated resistance to BET
            inhibitors in small cell lung cancer
  JOURNAL   JCI Insight 8 (5), e156657 (2023)
   PUBMED   36883564
  REMARK    GeneRIF: mTOR inhibition overcomes RSK3-mediated resistance to BET
            inhibitors in small cell lung cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 929)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   6  (bases 1 to 929)
  AUTHORS   Hsu,S.Y., Kaipia,A., Zhu,L. and Hsueh,A.J.
  TITLE     Interference of BAD (Bcl-xL/Bcl-2-associated death
            promoter)-induced apoptosis in mammalian cells by 14-3-3 isoforms
            and P11
  JOURNAL   Mol Endocrinol 11 (12), 1858-1867 (1997)
   PUBMED   9369453
REFERENCE   7  (bases 1 to 929)
  AUTHORS   Zha,J., Harada,H., Osipov,K., Jockel,J., Waksman,G. and
            Korsmeyer,S.J.
  TITLE     BH3 domain of BAD is required for heterodimerization with BCL-XL
            and pro-apoptotic activity
  JOURNAL   J Biol Chem 272 (39), 24101-24104 (1997)
   PUBMED   9305851
REFERENCE   8  (bases 1 to 929)
  AUTHORS   Wang,H.G., Rapp,U.R. and Reed,J.C.
  TITLE     Bcl-2 targets the protein kinase Raf-1 to mitochondria
  JOURNAL   Cell 87 (4), 629-638 (1996)
   PUBMED   8929532
REFERENCE   9  (bases 1 to 929)
  AUTHORS   Zha,J., Harada,H., Yang,E., Jockel,J. and Korsmeyer,S.J.
  TITLE     Serine phosphorylation of death agonist BAD in response to survival
            factor results in binding to 14-3-3 not BCL-X(L)
  JOURNAL   Cell 87 (4), 619-628 (1996)
   PUBMED   8929531
REFERENCE   10 (bases 1 to 929)
  AUTHORS   Yang,E., Zha,J., Jockel,J., Boise,L.H., Thompson,C.B. and
            Korsmeyer,S.J.
  TITLE     Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax
            and promotes cell death
  JOURNAL   Cell 80 (2), 285-291 (1995)
   PUBMED   7834748
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK291863.1 and AW014069.1.
            
            On Jun 2, 2019 this sequence version replaced NM_032989.2.
            
            Summary: The protein encoded by this gene is a member of the BCL-2
            family. BCL-2 family members are known to be regulators of
            programmed cell death. This protein positively regulates cell
            apoptosis by forming heterodimers with BCL-xL (B-cell
            lymphoma-extra large) and BCL-2, and reversing their death
            repressor activity. Proapoptotic activity of this protein is
            regulated through its phosphorylation. Protein kinases AKT and MAP
            kinase, as well as protein phosphatase calcineurin were found to be
            involved in the regulation of this protein. Alternative splicing of
            this gene results in two transcript variants which encode the same
            isoform. [provided by RefSeq, Dec 2019].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.200720.1,
                                           SRR7410570.472714.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2147920
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000309032.8/ ENSP00000309103.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-913               AK291863.1         40-952
            914-929             AW014069.1         19-34               c
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.1"
     gene            1..929
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /note="BCL2 associated agonist of cell death"
                     /db_xref="GeneID:572"
                     /db_xref="HGNC:HGNC:936"
                     /db_xref="MIM:603167"
     exon            1..35
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /inference="alignment:Splign:2.1.0"
     exon            36..230
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..550
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /note="BCL2-binding protein; BCL2-binding component 6;
                     BCL-X/BCL-2 binding protein; bcl2 antagonist of cell
                     death; BCL2-antagonist of cell death protein; bcl2-L-8;
                     bcl-2-like protein 8; bcl-2-binding component 6;
                     bcl-XL/Bcl-2-associated death promoter"
                     /codon_start=1
                     /product="bcl2-associated agonist of cell death"
                     /protein_id="NP_116784.1"
                     /db_xref="CCDS:CCDS8065.1"
                     /db_xref="GeneID:572"
                     /db_xref="HGNC:HGNC:936"
                     /db_xref="MIM:603167"
                     /translation="MFQIPEFEPSEQEDSSSAERGLGPSPAGDGPSGSGKHHRQAPGL
                     LWDASHQQEQPTSSSHHGGAGAVEIRSRHSSYPAGTEDDEGMGEEPSPFRGRSRSAPP
                     NLWAAQRYGRELRRMSDEFVDSFKKGLPRPKSAGTATQMRQSSSWTRVFQSWWDRNLG
                     RGSSAPSQ"
     exon            231..421
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /inference="alignment:Splign:2.1.0"
     exon            422..929
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      906..911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /note="hexamer: AATAAA"
     polyA_site      929
                     /gene="BAD"
                     /gene_synonym="BBC2; BCL2L8"
                     /note="major polyA site"
ORIGIN      
        1 aggcccgggt caggggcctc gagatcgggc ttgggcccag agcatgttcc agatcccaga
       61 gtttgagccg agtgagcagg aagactccag ctctgcagag aggggcctgg gccccagccc
      121 cgcaggggac gggccctcag gctccggcaa gcatcatcgc caggccccag gcctcctgtg
      181 ggacgccagt caccagcagg agcagccaac cagcagcagc catcatggag gcgctggggc
      241 tgtggagatc cggagtcgcc acagctccta ccccgcgggg acggaggacg acgaagggat
      301 gggggaggag cccagcccct ttcggggccg ctcgcgctcg gcgcccccca acctctgggc
      361 agcacagcgc tatggccgcg agctccggag gatgagtgac gagtttgtgg actcctttaa
      421 gaagggactt cctcgcccga agagcgcggg cacagcaacg cagatgcggc aaagctccag
      481 ctggacgcga gtcttccagt cctggtggga tcggaacttg ggcaggggaa gctccgcccc
      541 ctcccagtga ccttcgctcc acatcccgaa actccacccg ttcccactgc cctgggcagc
      601 catcttgaat atgggcggaa gtacttccct caggcctatg caaaaagagg atccgtgctg
      661 tctcctttgg agggagggct gacccagatt cccttccggt gcgtgtgaag ccacggaagg
      721 cttggtccca tcggaagttt tgggttttcc gcccacagcc gccggaagtg gctccgtggc
      781 cccgccctca ggctccgggc tttcccccag gcgcctgcgc taagtcgcga gccaggttta
      841 accgttgcgt caccgggacc cgagcccccg cgatgccctg ggggccgtgc tcactaccaa
      901 atgttaataa agcccgcgtc tgtgccgcc
//
