LOCUS       NM_012105               8567 bp    mRNA    linear   PRI 22-AUG-2024
DEFINITION  Homo sapiens beta-secretase 2 (BACE2), transcript variant a, mRNA.
ACCESSION   NM_012105
VERSION     NM_012105.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Maloney,B., Ge,Y.W., Greig,N.H. and Lahiri,D.K.
  TITLE     Characterization of the human beta-secretase 2 (BACE2) 5'-flanking
            region: identification of a 268-bp region as the basal BACE2
            promoter
  JOURNAL   J Mol Neurosci 29 (1), 81-99 (2006)
   PUBMED   16757812
  REMARK    GeneRIF: Data characterize the human beta-secretase 2 (BACE2)
            5'-flanking region.
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Sun,X., Wang,Y., Qing,H., Christensen,M.A., Liu,Y., Zhou,W.,
            Tong,Y., Xiao,C., Huang,Y., Zhang,S., Liu,X. and Song,W.
  TITLE     Distinct transcriptional regulation and function of the human BACE2
            and BACE1 genes
  JOURNAL   FASEB J 19 (7), 739-749 (2005)
   PUBMED   15857888
  REMARK    GeneRIF: data indicate that despite being homologous in amino acid
            sequence, beta-site beta amyloid precursor protein cleaving enzyme
            2(BACE2) and BACE1 have distinct functions and transcriptional
            regulation
REFERENCE   3  (bases 1 to 8567)
  AUTHORS   Yan,R., Munzner,J.B., Shuck,M.E. and Bienkowski,M.J.
  TITLE     BACE2 functions as an alternative alpha-secretase in cells
  JOURNAL   J Biol Chem 276 (36), 34019-34027 (2001)
   PUBMED   11423558
REFERENCE   4  (bases 1 to 8567)
  AUTHORS   Hussain,I., Christie,G., Schneider,K., Moore,S. and Dingwall,C.
  TITLE     Prodomain processing of Asp1 (BACE2) is autocatalytic
  JOURNAL   J Biol Chem 276 (26), 23322-23328 (2001)
   PUBMED   11316808
REFERENCE   5  (bases 1 to 8567)
  AUTHORS   Hussain,I., Powell,D.J., Howlett,D.R., Chapman,G.A., Gilmour,L.,
            Murdock,P.R., Tew,D.G., Meek,T.D., Chapman,C., Schneider,K.,
            Ratcliffe,S.J., Tattersall,D., Testa,T.T., Southan,C., Ryan,D.M.,
            Simmons,D.L., Walsh,F.S., Dingwall,C. and Christie,G.
  TITLE     ASP1 (BACE2) cleaves the amyloid precursor protein at the
            beta-secretase site
  JOURNAL   Mol Cell Neurosci 16 (5), 609-619 (2000)
   PUBMED   11083922
REFERENCE   6  (bases 1 to 8567)
  AUTHORS   Xin,H., Stephans,J.C., Duan,X., Harrowe,G., Kim,E., Grieshammer,U.,
            Kingsley,C. and Giese,K.
  TITLE     Identification of a novel aspartic-like protease differentially
            expressed in human breast cancer cell lines
  JOURNAL   Biochim Biophys Acta 1501 (2-3), 125-137 (2000)
   PUBMED   10838186
REFERENCE   7  (bases 1 to 8567)
  AUTHORS   Acquati,F., Accarino,M., Nucci,C., Fumagalli,P., Jovine,L.,
            Ottolenghi,S. and Taramelli,R.
  TITLE     The gene encoding DRAP (BACE2), a glycosylated transmembrane
            protein of the aspartic protease family, maps to the down critical
            region
  JOURNAL   FEBS Lett 468 (1), 59-64 (2000)
   PUBMED   10683441
REFERENCE   8  (bases 1 to 8567)
  AUTHORS   Lin,X., Koelsch,G., Wu,S., Downs,D., Dashti,A. and Tang,J.
  TITLE     Human aspartic protease memapsin 2 cleaves the beta-secretase site
            of beta-amyloid precursor protein
  JOURNAL   Proc Natl Acad Sci U S A 97 (4), 1456-1460 (2000)
   PUBMED   10677483
REFERENCE   9  (bases 1 to 8567)
  AUTHORS   Solans,A., Estivill,X. and de La Luna,S.
  TITLE     A new aspartyl protease on 21q22.3, BACE2, is highly similar to
            Alzheimer's amyloid precursor protein beta-secretase
  JOURNAL   Cytogenet Cell Genet 89 (3-4), 177-184 (2000)
   PUBMED   10965118
REFERENCE   10 (bases 1 to 8567)
  AUTHORS   Yan,R., Bienkowski,M.J., Shuck,M.E., Miao,H., Tory,M.C.,
            Pauley,A.M., Brashier,J.R., Stratman,N.C., Mathews,W.R., Buhl,A.E.,
            Carter,D.B., Tomasselli,A.G., Parodi,L.A., Heinrikson,R.L. and
            Gurney,M.E.
  TITLE     Membrane-anchored aspartyl protease with Alzheimer's disease
            beta-secretase activity
  JOURNAL   Nature 402 (6761), 533-537 (1999)
   PUBMED   10591213
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF178532.1, AF200342.1,
            AF188276.1 and AL773578.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012105.4.
            
            Summary: This gene encodes an integral membrane glycoprotein that
            functions as an aspartic protease. The encoded protein cleaves
            amyloid precursor protein into amyloid beta peptide, which is a
            critical step in the etiology of Alzheimer's disease and Down
            syndrome. The protein precursor is further processed into an active
            mature peptide. Alternative splicing results in multiple transcript
            variants. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (a) represents the longest
            transcript and encodes the longest isoform (A).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF178532.1, SRR1803615.14606.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330333.11/ ENSP00000332979.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1414              AF178532.1         360-1773
            1415-1897           AF200342.1         1311-1793
            1898-2631           AF188276.1         2107-2840
            2632-8567           AL773578.1         11078-17013
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q22.2-q22.3"
     gene            1..8567
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /note="beta-secretase 2"
                     /db_xref="GeneID:25825"
                     /db_xref="HGNC:HGNC:934"
                     /db_xref="MIM:605668"
     exon            1..416
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..1661
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /EC_number="3.4.23.45"
                     /note="isoform A preproprotein is encoded by transcript
                     variant a; aspartyl protease 1; membrane-associated
                     aspartic protease 1; memapsin-1; Down syndrome region
                     aspartic protease; 56 kDa aspartic-like protease;
                     beta-site amyloid beta A4 precursor protein-cleaving
                     enzyme 2; transmembrane aspartic proteinase Asp1;
                     theta-secretase; beta-site APP-cleaving enzyme 2;
                     SLCO3A1/BACE2 fusion"
                     /codon_start=1
                     /product="beta-secretase 2 isoform A preproprotein"
                     /protein_id="NP_036237.2"
                     /db_xref="CCDS:CCDS13668.1"
                     /db_xref="GeneID:25825"
                     /db_xref="HGNC:HGNC:934"
                     /db_xref="MIM:605668"
                     /translation="MGALARALLLPLLAQWLLRAAPELAPAPFTLPLRVAAATNRVVA
                     PTPGPGTPAERHADGLALALEPALASPAGAANFLAMVDNLQGDSGRGYYLEMLIGTPP
                     QKLQILVDTGSSNFAVAGTPHSYIDTYFDTERSSTYRSKGFDVTVKYTQGSWTGFVGE
                     DLVTIPKGFNTSFLVNIATIFESENFFLPGIKWNGILGLAYATLAKPSSSLETFFDSL
                     VTQANIPNVFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQI
                     EILKLEIGGQSLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARASLIPEFSD
                     GFWTGSQLACWTNSETPWSYFPKISIYLRDENSSRSFRITILPQLYIQPMMGAGLNYE
                     CYRFGISPSTNALVIGATVMEGFYVIFDRAQKRVGFAASPCAEIAGAAVSEISGPFST
                     EDVASNCVPAQSLSEPILWIVSYALMSVCGAILLVLIVLLLLPFRCQRRPRDPEVVND
                     ESSLVRHRWK"
     exon            417..505
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            506..722
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            723..851
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            852..986
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1088
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1238
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1407
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..8567
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1867..1872
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /note="hexamer: ATTAAA"
     polyA_site      1897
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /note="major polyA site"
     regulatory      2600..2605
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /note="hexamer: AATAAA"
     polyA_site      2626
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /note="major polyA site"
     regulatory      8550..8555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
                     /note="hexamer: AATAAA"
     polyA_site      8567
                     /gene="BACE2"
                     /gene_synonym="AEPLC; ALP56; ASP1; ASP21; BAE2; CDA13;
                     CEAP1; DRAP"
ORIGIN      
        1 atccctgccc gcagccccgc gcgccggccg agtcgctgag ccgcggctgc cggacgggac
       61 gggaccggct aggctgggcg cgccccccgg gccccgccgt gggcatgggc gcactggccc
      121 gggcgctgct gctgcctctg ctggcccagt ggctcctgcg cgccgccccg gagctggccc
      181 ccgcgccctt cacgctgccc ctccgggtgg ccgcggccac gaaccgcgta gttgcgccca
      241 ccccgggacc cgggacccct gccgagcgcc acgccgacgg cttggcgctc gccctggagc
      301 ctgccctggc gtcccccgcg ggcgccgcca acttcttggc catggtagac aacctgcagg
      361 gggactctgg ccgcggctac tacctggaga tgctgatcgg gacccccccg cagaagctac
      421 agattctcgt tgacactgga agcagtaact ttgccgtggc aggaaccccg cactcctaca
      481 tagacacgta ctttgacaca gagaggtcta gcacataccg ctccaagggc tttgacgtca
      541 cagtgaagta cacacaagga agctggacgg gcttcgttgg ggaagacctc gtcaccatcc
      601 ccaaaggctt caatacttct tttcttgtca acattgccac tatttttgaa tcagagaatt
      661 tctttttgcc tgggattaaa tggaatggaa tacttggcct agcttatgcc acacttgcca
      721 agccatcaag ttctctggag accttcttcg actccctggt gacacaagca aacatcccca
      781 acgttttctc catgcagatg tgtggagccg gcttgcccgt tgctggatct gggaccaacg
      841 gaggtagtct tgtcttgggt ggaattgaac caagtttgta taaaggagac atctggtata
      901 cccctattaa ggaagagtgg tactaccaga tagaaattct gaaattggaa attggaggcc
      961 aaagccttaa tctggactgc agagagtata acgcagacaa ggccatcgtg gacagtggca
     1021 ccacgctgct gcgcctgccc cagaaggtgt ttgatgcggt ggtggaagct gtggcccgcg
     1081 catctctgat tccagaattc tctgatggtt tctggactgg gtcccagctg gcgtgctgga
     1141 cgaattcgga aacaccttgg tcttacttcc ctaaaatctc catctacctg agagacgaga
     1201 actccagcag gtcattccgt atcacaatcc tgcctcagct ttacattcag cccatgatgg
     1261 gggccggcct gaattatgaa tgttaccgat tcggcatttc cccatccaca aatgcgctgg
     1321 tgatcggtgc cacggtgatg gagggcttct acgtcatctt cgacagagcc cagaagaggg
     1381 tgggcttcgc agcgagcccc tgtgcagaaa ttgcaggtgc tgcagtgtct gaaatttccg
     1441 ggcctttctc aacagaggat gtagccagca actgtgtccc cgctcagtct ttgagcgagc
     1501 ccattttgtg gattgtgtcc tatgcgctca tgagcgtctg tggagccatc ctccttgtct
     1561 taatcgtcct gctgctgctg ccgttccggt gtcagcgtcg cccccgtgac cctgaggtcg
     1621 tcaatgatga gtcctctctg gtcagacatc gctggaaatg aatagccagg cctgacctca
     1681 agcaaccatg aactcagcta ttaagaaaat cacatttcca gggcagcagc cgggatcgat
     1741 ggtggcgctt tctcctgtgc ccacccgtct tcaatctctg ttctgctccc agatgccttc
     1801 tagattcact gtcttttgat tcttgatttt caagctttca aatcctccct acttccaaga
     1861 aaaataatta aaaaaaaaac ttcattctaa accaaaacag agtggattgg gctgcaggct
     1921 ctatggggtt cgttatgcca aagtgtctac atgtgccacc aacataaaac aaaaccaagc
     1981 cttggctcgt tctcttctct cttcaatctc tggaaaaata agtacatata gttgataacc
     2041 cctcttagct tacaggaagc tttttgtatt aattgccttt gaggttattt tccgccagac
     2101 ctcaacctgg gtcaaagtgg tacaggaagg cttgcagtat gatggcagga gaatcagcct
     2161 ggggcctggg gatgtaacca agctgtaccc ttgagacctg gaaccagagc cacaggcccc
     2221 ttttgtgggt ttctctgtgc tctgaatggg agccagaatt cactaggagg tcatcaaccg
     2281 atggtcctca caagcctctt ctgaagatgg aaggcctttt gcccgttgag gtagagggga
     2341 aggaaatctc ctcttttgta cccaatactt atgttgtatt gttggtgcga aagtaaaaac
     2401 actacctctt ttgagacttt gcccagggtc ctgtgcctgg atgggggtgc aggcagcctt
     2461 gaccacggct gttcccctca cccaaaagaa ttatcatccc aacagccaag acccaacagg
     2521 tgctgaactg tgcatcaacc aggaagagtt ctatccccaa gctggccact atcacatatg
     2581 cttactcttg cttaaaatta ataaatcatg ttttgatgag aaaaaactat tctatttcac
     2641 tagcttagtt gtctcttttt ccaaatcttc tctggaagta ggttggctat taccctgttg
     2701 ggaaacaggg aaatggcctg atgcccctat ttctgaccag ctgtcaggga aggaagatgc
     2761 aagatgtgca gaagacacaa ggtaacgtct acttatcccc gtgcttcgaa accgtgtgtt
     2821 tccctttgca gaactggtta gcctcaagca tacccatcca atcagcttaa attgttcagc
     2881 agttgacctc tgtgcttctt cctttggggg tgtttaatct ttggtaacag ttgtgcagtg
     2941 gtagccttat ttcggggggc agtccctact ttctgtcact tatcgaggcc tttgccaaag
     3001 aaaagtggaa gccgtttcct tgacatagcc ttatatgacc tgatgtcatt aggagaagag
     3061 ttaaaagaca gtatcccctc tctcctgctt gtgtgccaag ttcagtgttc cctttgcatt
     3121 tttttggggt gggttggcta tattacagaa ttccaccaaa agtgctaagt gtcccctcct
     3181 gcagaagtat cgtttgtatt cacagtaggc tacgtgtgga tgtttcagcc atctgctctg
     3241 ctccctgggt ccccgggtca ccaccatact gagaaatata ggactctgcc aaagagacgt
     3301 ggaggccacg tcggcttgtc atggtgtcct ggaagactct ccagcagcca gaagaacctt
     3361 ctgtgctgtt ttcactagag aagatttgag ttgcagcctc ttggaaagaa catgacggca
     3421 cggaacggga ggagggggct cttgtatcag ggcccgttgt cacatccgct ctcagcttgt
     3481 tgaaaactca taatctcaga atgcatttca cacggagaga tgtctggaag tccaattctc
     3541 atcccattgg ctttttccgt ggttgctgtt ttgtgggccg ctttgcacag gagtgaagcc
     3601 aacaccccta gattgtgcct tcacccttgc tgtgaaaaat tcaaagccct gaaataagag
     3661 gagggaatcg cagacatgcc ctgattagtt ttcctgctta tttcttcata caggcatctt
     3721 ctgttccgtt gtattgtgtt aggtttcaga ggcggggagc ttcctcctgc agcagatgaa
     3781 caggcatagt tcttaaaata caggaaagta acaagtgagg tttccaggta gagtaggtga
     3841 atgaagtttg ccgtcatgct ctgtatttca tatcagattt ctttatcttt tcatatcgca
     3901 tgtcattgga tgtgacattt tgatgaaaat actgaaacta caaaagcata acttcgcaac
     3961 aagaatgaga tagacaattg aatgtgggac atttttctgg catgtcttgc tgctgagaat
     4021 cggacttcca gtcaaaaccc tgttagtgac acccacctcc tccagttatg caaattgatg
     4081 cttagcttgg caagctttca aggaggtttg cttggaacag gaaaggaatt aacaaccctt
     4141 tgagggtgtc atttttacct tgtaatacac tgtggaaaat tctggaagac ggtccttgcc
     4201 tgaaaaggtg caacttagca ttaggttgtt atcagaaacc ttgtcaagtc tgatgggaaa
     4261 tgaatagatg ctgctttatt tccttgataa cttctttgga agtctgaaat aaatcctgcc
     4321 ttcagtaact gaaaccattt attaaaaata gcaagaaaca agatggggac caattctatt
     4381 tttattcttg ggaatttgaa agagccacaa gtgattggtg tccgatctcc cagcatatac
     4441 tgtacactat tttgtatttc tcctgaacca aaaccaagta cttagcactc aagaaacaga
     4501 tgccctgtgt gtgaccgagg ggagtgtttt tccacaacag gttccacttc tgaacacttg
     4561 gaaggacctt gcctgattca tggtgaaaca cagaggtttt ggttggagat aaatttgggg
     4621 tcagttactg ttcaaaattg tgaaagtagt tcccaggaag acagcattag tgactccgca
     4681 gtgctttggg taatagattt tgaaaagcaa ttgtgagttg gggattccat catttcattt
     4741 aagaggtgaa gagggattta attatttaat acgccgggtg caaacttgtt aaattcctct
     4801 ttccctgtcc ctgcttggtg gagctgccac gtgtggttca gttctaagtt ctggactagt
     4861 gccactcgat atcattggga ataaatcgtt gttcaacagt ctgttccaca aacaacagta
     4921 attgaatttc acagacttta tcctgattag tggaaatcaa ataatggagt ggaatcccca
     4981 tgtgttttct cttgcacaaa cttattagac ttgattagaa aactgtttgg aggaaaaact
     5041 tgccagtaag ttttttggtt gttgttttgt tttttttgaa aaacgacgca acaccataaa
     5101 gtgtaaaatt aggggttctg gccaagagag gtactagatt tttttaatgc cgagaaaagt
     5161 ccatgccaca gaagttattg tgtatgtctc tagggagctt taagaatttc acgtttgaac
     5221 tttacagctg tttaccaatt aaatggcaag ctggaaaaat acattttgag aggggtagaa
     5281 agaataagaa tataattaaa agtatgctaa caaaataagc agtacatatt tttttagaaa
     5341 atgcagttta ggccaggcac ggtagctcgt gcctgtaatc ccagcacttt gggaggccga
     5401 ggcaggtgga tcacctgagg tcaggagttt gagaccagcc tggacaacat ggtgaaaccc
     5461 cgtctctact aaaaatacaa aaaattagcc aacgatggtg atgcctgtct gtaatcccag
     5521 ctactctgga ggctgaggca ggagaatcgc ttgaaccggg gaggcagagg ttgtagtgag
     5581 ccaagattgc gccactgcac tccagcctgg gcaacagagc aagactccgt ctcaaaaaag
     5641 gaaagaaaat gcagtttcat tccagccatc cttagatttc ttttcccctt aaagtggctg
     5701 aatccattgg agatgtgatt tattttgcag atccaccaat tccaaacttc aggaatattc
     5761 aagcgggagt catgtgtaag tgacagcttt gacaaagaga gactgtggct gggtggcccg
     5821 ggtgttgccc acgcggagca aggattggtt cattcaacgg atggagagta caggatggct
     5881 gggacctggg aagttccaga atctgaaaag agggcagggc aagaaggagc cagaagagag
     5941 gtcccacccc tcagttggca gtggggcacg gaggtccgca tcagccaaga acgtccccag
     6001 gatgccgtgg gcacctcatt tcaccccacg tttttggcac agtctacttt ttaggtattt
     6061 tgtaaaggaa cttctcagga ttgattattt gccagaggaa caattaggcc aagagaaact
     6121 tgcttgacag ccatgtgtaa aacacgtctg tcctaatcaa actagagatg cctcaaggcc
     6181 cacgcaggac acaggcaggt tcaaggtcat gcacctggaa atttgtaggg gagggtcaag
     6241 caactgaggc caaagggggc cggtaaagtg gcttctgggg tctgagcatc tggaaggctc
     6301 ctggcagcag ttacctgaca gtgtcgttga ggggcacggc agctgggccc tcaccgtttc
     6361 cgccagctct gctgagccag cccagcccat gcattccgaa catccgacag cttctttctc
     6421 cagaatgtct tctgtcagag acttccagga aaggagctcc ctcctgtctc actgctgtca
     6481 gcgtgggagc cctgctcccc aggctccacg tccccggaag gtccatcttg tcctggagca
     6541 cacccagccc cattcagggt cagtgcagga gggaacaggg aatctgcatt tcagtaagct
     6601 ccccaggtga ggctgcagag cagaaaccac ggctttcaga cgtgagcagg ctccctgggc
     6661 tcttggtgtc accctgttag ccacatctgc ggaatgtgga aggcagaaca tagccacgct
     6721 gcttagtgct gccagcgaac ctcaggttgc atcagtggct ggaatctatc acagctgttg
     6781 gggatacgta actcccagca ataaatccag tattttcttc cctgccctga gcttcctctg
     6841 ggtttcttat cagcagctat tacacacaga tgtccagtgc ttctaaacta aattgtactg
     6901 tgtaactttt acatcacact tgtgtgctta aaaagcacac cgtgggtagt gactgggcca
     6961 agagacatca caaagcttcc tgataaattt gaatccagcc acggcaagag cgacgtgcgg
     7021 gtacctggtc cctgatgctt gggtgccccg ttgagcctcg ggtacttctt ggacactatt
     7081 gtgagagcag ccatcctggt gtctctgcca gcatcttggg gaacgctgtg atggtaaaat
     7141 atctgtctct cctctgggaa tcagggcagc cctgagaagt gagcagttcc ctttacagat
     7201 ggataattgt ttgtgtaaga ccaaccagat aaagtccaaa ctgtgtagaa cacacgtgtg
     7261 cctcacacat agccatctga gtaaaatatt ttttccaatg tagatttata attagaaatg
     7321 acagatttat aatatagact tcagtaagtt gggcacagct gtcactttcg tggtatcaat
     7381 aaaaggctag aaaccttggg tgtccttggc aacctagcct ttgacattga tgtttttcca
     7441 taggattttc ttcatttggg ttggaataaa aatgcatttt tattcacaag gcacagacag
     7501 ataagaatat cataagcagg gaagtgtctc caaaggtcag gacatatgtt tttctgttga
     7561 gtgctatatg tggaggtcat tgcaagttcc ctgatatgag tatggtttcg cttgctacat
     7621 tgtgcctatt aaagtaaaat tttacacaag cctcgcattt ctaagattag tgttcctgaa
     7681 tgaaatgtta agaaaacatt aaaagattat ctctttttaa gatggaggaa aaaaagtgaa
     7741 caaagctaat taatctatca tgaaaattgc acaaaataac atttcttaac aaatttaata
     7801 caattttgtg ttctttgttg ctagtggtat aaaacgagat ttttttccct catttttctc
     7861 attgtagatg tcatctctca catttatatc agtgaggttt gaaattctgt gtagcagtta
     7921 ctcagcacat atgagagggc agcgaatgaa tgagatttgt catgtgctaa taaaagctga
     7981 atttttgtaa tctaaaatga tgtattttct actattgctg ttaatttgca ttgttaaaaa
     8041 ttcttaaagt ttaatatgtt atgttcagtc attgaaagcg accactcatt tttttcttaa
     8101 agttgatgcc ttttctgctg tgctagagtc agtattttgc ttctggcagg agagctgcaa
     8161 actgtgtatc ctcaaacaga tgcaaaaagt agtgctttgc aaaacgtttg ttttctgttt
     8221 atctcagatt aacatccttt aatacaagtt tcttaagtgt aacttgtatt tctgaaaatg
     8281 cttaaaatta ttttatattt ccctttggga atttttctct atttccagca cgctgatttg
     8341 atttaaaaat gtaataagac caagagttgg agtaaaggga tattcattcc atgttaaaag
     8401 tggcttcata gctactgaca aatgtctgaa ctattgtcgt gcccttcaaa actggagttt
     8461 tctaaaataa tcttattttt atacttgtat gttccagcaa tttaagatat ataccattga
     8521 aagggaaata aaacattttt gtttatttga ataaataata ctcccaa
//
