LOCUS       NM_012104               5835 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens beta-secretase 1 (BACE1), transcript variant a, mRNA.
ACCESSION   NM_012104
VERSION     NM_012104.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Katusic,Z.S., d'Uscio,L.V. and He,T.
  TITLE     Cerebrovascular Endothelial Dysfunction: Role of BACE1
  JOURNAL   Arterioscler Thromb Vasc Biol 44 (8), 1737-1747 (2024)
   PUBMED   38868939
  REMARK    GeneRIF: Cerebrovascular Endothelial Dysfunction: Role of BACE1.
            Review article
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Saraceno,C., Cervellati,C., Trentini,A., Crescenti,D.,
            Longobardi,A., Geviti,A., Bonfiglio,N.S., Bellini,S., Nicsanu,R.,
            Fostinelli,S., Mola,G., Riccetti,R., Moretti,D.V., Zanetti,O.,
            Binetti,G., Zuliani,G. and Ghidoni,R.
  TITLE     Serum Beta-Secretase 1 Activity Is a Potential Marker for the
            Differential Diagnosis between Alzheimer's Disease and
            Frontotemporal Dementia: A Pilot Study
  JOURNAL   Int J Mol Sci 25 (15), 8354 (2024)
   PUBMED   39125924
  REMARK    GeneRIF: Serum Beta-Secretase 1 Activity Is a Potential Marker for
            the Differential Diagnosis between Alzheimer's Disease and
            Frontotemporal Dementia: A Pilot Study.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5835)
  AUTHORS   Gao,F., Zhang,M., Wang,Q., Ni,M., Liu,C., Deng,K., Xie,Q., Wang,S.,
            Shi,J. and Shen,Y.
  CONSRTM   For CANDI Consortium
  TITLE     Associations of CSF BACE1 with amyloid pathology,
            neurodegeneration, and cognition in Alzheimer's disease
  JOURNAL   Acta Neuropathol 147 (1), 97 (2024)
   PUBMED   38856925
  REMARK    GeneRIF: Associations of CSF BACE1 with amyloid pathology,
            neurodegeneration, and cognition in Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5835)
  AUTHORS   Kandalepas,P.C. and Vassar,R.
  TITLE     The normal and pathologic roles of the Alzheimer's beta-secretase,
            BACE1
  JOURNAL   Curr Alzheimer Res 11 (5), 441-449 (2014)
   PUBMED   24893886
  REMARK    Review article
REFERENCE   5  (bases 1 to 5835)
  AUTHORS   De Pietri Tonelli,D., Mihailovich,M., Di Cesare,A., Codazzi,F.,
            Grohovaz,F. and Zacchetti,D.
  TITLE     Translational regulation of BACE-1 expression in neuronal and
            non-neuronal cells
  JOURNAL   Nucleic Acids Res 32 (5), 1808-1817 (2004)
   PUBMED   15034149
  REMARK    GeneRIF: Alternative splicing in the BACE-1 5'UTR affects the
            efficiency of translation initiation
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5835)
  AUTHORS   Tanahashi,H. and Tabira,T.
  TITLE     Three novel alternatively spliced isoforms of the human beta-site
            amyloid precursor protein cleaving enzyme (BACE) and their effect
            on amyloid beta-peptide production
  JOURNAL   Neurosci Lett 307 (1), 9-12 (2001)
   PUBMED   11516562
REFERENCE   7  (bases 1 to 5835)
  AUTHORS   Sinha,S., Anderson,J.P., Barbour,R., Basi,G.S., Caccavello,R.,
            Davis,D., Doan,M., Dovey,H.F., Frigon,N., Hong,J.,
            Jacobson-Croak,K., Jewett,N., Keim,P., Knops,J., Lieberburg,I.,
            Power,M., Tan,H., Tatsuno,G., Tung,J., Schenk,D., Seubert,P.,
            Suomensaari,S.M., Wang,S., Walker,D., Zhao,J., McConlogue,L. and
            John,V.
  TITLE     Purification and cloning of amyloid precursor protein
            beta-secretase from human brain
  JOURNAL   Nature 402 (6761), 537-540 (1999)
   PUBMED   10591214
REFERENCE   8  (bases 1 to 5835)
  AUTHORS   Yan,R., Bienkowski,M.J., Shuck,M.E., Miao,H., Tory,M.C.,
            Pauley,A.M., Brashier,J.R., Stratman,N.C., Mathews,W.R., Buhl,A.E.,
            Carter,D.B., Tomasselli,A.G., Parodi,L.A., Heinrikson,R.L. and
            Gurney,M.E.
  TITLE     Membrane-anchored aspartyl protease with Alzheimer's disease
            beta-secretase activity
  JOURNAL   Nature 402 (6761), 533-537 (1999)
   PUBMED   10591213
REFERENCE   9  (bases 1 to 5835)
  AUTHORS   Vassar,R., Bennett,B.D., Babu-Khan,S., Kahn,S., Mendiaz,E.A.,
            Denis,P., Teplow,D.B., Ross,S., Amarante,P., Loeloff,R., Luo,Y.,
            Fisher,S., Fuller,J., Edenson,S., Lile,J., Jarosinski,M.A.,
            Biere,A.L., Curran,E., Burgess,T., Louis,J.C., Collins,F.,
            Treanor,J., Rogers,G. and Citron,M.
  TITLE     Beta-secretase cleavage of Alzheimer's amyloid precursor protein by
            the transmembrane aspartic protease BACE
  JOURNAL   Science 286 (5440), 735-741 (1999)
   PUBMED   10531052
REFERENCE   10 (bases 1 to 5835)
  AUTHORS   Urmoneit,B., Reinsch,C., Turner,J., Czech,C., Beyreuther,K. and
            Dyrks,T.
  TITLE     Inhibition of beta A4 production by specific modulation of
            beta-secretase activity
  JOURNAL   J Mol Neurosci 6 (1), 23-32 (1995)
   PUBMED   8562317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP000892.4 and KC877486.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 19, 2020 this sequence version replaced NM_012104.5.
            
            Summary: This gene encodes a member of the peptidase A1 family of
            aspartic proteases. Alternative splicing results in multiple
            transcript variants, at least one of which encodes a preproprotein
            that is proteolytically processed to generate the mature protease.
            This transmembrane protease catalyzes the first step in the
            formation of amyloid beta peptide from amyloid precursor protein.
            Amyloid beta peptides are the main constituent of amyloid beta
            plaques, which accumulate in the brains of human Alzheimer's
            disease patients. [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (a) is the longest transcript and
            it encodes the longest isoform (A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF201468.1, BC065492.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000313005.11/ ENSP00000318585.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            regulatory uORF        :: PMID: 15034149
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-722               AP000892.4         114018-114739       c
            723-811             AP000892.4         95356-95444         c
            812-1028            AP000892.4         93614-93830         c
            1029-1166           AP000892.4         92354-92491         c
            1167-1301           AP000892.4         91537-91671         c
            1302-1403           AP000892.4         90195-90296         c
            1404-1553           AP000892.4         89383-89532         c
            1554-1725           AP000892.4         88971-89142         c
            1726-2152           KC877486.1         77-503
            2153-5835           AP000892.4         84181-87863         c
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.3"
     gene            1..5835
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /note="beta-secretase 1"
                     /db_xref="GeneID:23621"
                     /db_xref="HGNC:HGNC:933"
                     /db_xref="MIM:604252"
     exon            1..722
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     regulatory      56..67
                     /regulatory_class="uORF"
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:15034149]"
                     /note="this uORF is predicted to encode a 3 aa peptide
                     (PMID:15034149)"
     regulatory      158..230
                     /regulatory_class="uORF"
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:15034149]"
                     /note="this uORF is predicted to encode a 23 aa peptide
                     (PMID:15034149)"
     regulatory      225..230
                     /regulatory_class="uORF"
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:15034149]"
                     /note="this uORF is predicted to encode 1
                     aa(PMID:15034149)"
     regulatory      346..369
                     /regulatory_class="uORF"
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:15034149]"
                     /note="this uORF is predicted to encode a 7 aa peptide
                     (PMID:15034149)"
     CDS             462..1967
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /EC_number="3.4.23.46"
                     /note="isoform A preproprotein is encoded by transcript
                     variant a; beta-site amyloid beta A4 precursor
                     protein-cleaving enzyme; APP beta-secretase; aspartyl
                     protease 2; memapsin-2; membrane-associated aspartic
                     protease 2; transmembrane aspartic proteinase Asp2; asp 2;
                     beta-site APP cleaving enzyme 1; beta-site APP-cleaving
                     enzyme"
                     /codon_start=1
                     /product="beta-secretase 1 isoform A preproprotein"
                     /protein_id="NP_036236.1"
                     /db_xref="CCDS:CCDS8383.1"
                     /db_xref="GeneID:23621"
                     /db_xref="HGNC:HGNC:933"
                     /db_xref="MIM:604252"
                     /translation="MAQALPWLLLWMGAGVLPAHGTQHGIRLPLRSGLGGAPLGLRLP
                     RETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVG
                     AAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANI
                     AAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGA
                     GFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKM
                     DCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAG
                     TTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGT
                     VMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQT
                     DESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK"
     exon            723..811
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            812..1028
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1166
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1301
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1403
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1553
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1725
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..5835
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3701..3706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /note="hexamer: AATAAA"
     polyA_site      3723
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
     regulatory      5810..5815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /note="hexamer: AGTAAA"
     polyA_site      5835
                     /gene="BACE1"
                     /gene_synonym="ASP2; BACE; HSPC104"
                     /note="major polyA site"
ORIGIN      
        1 acaagtcttt ccgcctcccc agcccgcccg ggagctgcga gccgcgagct ggattatggt
       61 ggcctgagca gccaacgcag ccgcaggagc ccggagccct tgcccctgcc cgcgccgccg
      121 cccgccgggg ggaccaggga agccgccacc ggcccgccat gcccgcccct cccagccccg
      181 ccgggagccc gcgcccgctg cccaggctgg ccgccgccgt gccgatgtag cgggctccgg
      241 atcccagcct ctcccctgct cccgtgctct gcggatctcc cctgaccgct ctccacagcc
      301 cggacccggg ggctggccca gggccctgca ggccctggcg tcctgatgcc cccaagctcc
      361 ctctcctgag aagccaccag caccacccag acttgggggc aggcgccagg gacggacgtg
      421 ggccagtgcg agcccagagg gcccgaaggc cggggcccac catggcccaa gccctgccct
      481 ggctcctgct gtggatgggc gcgggagtgc tgcctgccca cggcacccag cacggcatcc
      541 ggctgcccct gcgcagcggc ctggggggcg cccccctggg gctgcggctg ccccgggaga
      601 ccgacgaaga gcccgaggag cccggccgga ggggcagctt tgtggagatg gtggacaacc
      661 tgaggggcaa gtcggggcag ggctactacg tggagatgac cgtgggcagc cccccgcaga
      721 cgctcaacat cctggtggat acaggcagca gtaactttgc agtgggtgct gccccccacc
      781 ccttcctgca tcgctactac cagaggcagc tgtccagcac ataccgggac ctccggaagg
      841 gtgtgtatgt gccctacacc cagggcaagt gggaagggga gctgggcacc gacctggtaa
      901 gcatccccca tggccccaac gtcactgtgc gtgccaacat tgctgccatc actgaatcag
      961 acaagttctt catcaacggc tccaactggg aaggcatcct ggggctggcc tatgctgaga
     1021 ttgccaggcc tgacgactcc ctggagcctt tctttgactc tctggtaaag cagacccacg
     1081 ttcccaacct cttctccctg cagctttgtg gtgctggctt ccccctcaac cagtctgaag
     1141 tgctggcctc tgtcggaggg agcatgatca ttggaggtat cgaccactcg ctgtacacag
     1201 gcagtctctg gtatacaccc atccggcggg agtggtatta tgaggtgatc attgtgcggg
     1261 tggagatcaa tggacaggat ctgaaaatgg actgcaagga gtacaactat gacaagagca
     1321 ttgtggacag tggcaccacc aaccttcgtt tgcccaagaa agtgtttgaa gctgcagtca
     1381 aatccatcaa ggcagcctcc tccacggaga agttccctga tggtttctgg ctaggagagc
     1441 agctggtgtg ctggcaagca ggcaccaccc cttggaacat tttcccagtc atctcactct
     1501 acctaatggg tgaggttacc aaccagtcct tccgcatcac catccttccg cagcaatacc
     1561 tgcggccagt ggaagatgtg gccacgtccc aagacgactg ttacaagttt gccatctcac
     1621 agtcatccac gggcactgtt atgggagctg ttatcatgga gggcttctac gttgtctttg
     1681 atcgggcccg aaaacgaatt ggctttgctg tcagcgcttg ccatgtgcac gatgagttca
     1741 ggacggcagc ggtggaaggc ccttttgtca ccttggacat ggaagactgt ggctacaaca
     1801 ttccacagac agatgagtca accctcatga ccatagccta tgtcatggct gccatctgcg
     1861 ccctcttcat gctgccactc tgcctcatgg tgtgtcagtg gcgctgcctc cgctgcctgc
     1921 gccagcagca tgatgacttt gctgatgaca tctccctgct gaagtgagga ggcccatggg
     1981 cagaagatag agattcccct ggaccacacc tccgtggttc actttggtca caagtaggag
     2041 acacagatgg cacctgtggc cagagcacct caggaccctc cccacccacc aaatgcctct
     2101 gccttgatgg agaaggaaaa ggctggcaag gtgggttcca gggactgtac ctgtaggaaa
     2161 cagaaaagag aagaaagaag cactctgctg gcgggaatac tcttggtcac ctcaaattta
     2221 agtcgggaaa ttctgctgct tgaaacttca gccctgaacc tttgtccacc attcctttaa
     2281 attctccaac ccaaagtatt cttcttttct tagtttcaga agtactggca tcacacgcag
     2341 gttaccttgg cgtgtgtccc tgtggtaccc tggcagagaa gagaccaagc ttgtttccct
     2401 gctggccaaa gtcagtagga gaggatgcac agtttgctat ttgctttaga gacagggact
     2461 gtataaacaa gcctaacatt ggtgcaaaga ttgcctcttg aattaaaaaa aaaaactaga
     2521 ttgactattt atacaaatgg gggcggctgg aaagaggaga aggagaggga gtacaaagac
     2581 agggaatagt gggatcaaag ctaggaaagg cagaaacaca accactcacc agtcctagtt
     2641 ttagacctca tctccaagat agcatcccat ctcagaagat gggtgttgtt ttcaatgttt
     2701 tcttttctgt ggttgcagcc tgaccaaaag tgagatggga agggcttatc tagccaaaga
     2761 gctctttttt agctctctta aatgaagtgc ccactaagaa gttccactta acacatgaat
     2821 ttctgccata ttaatttcat tgtctctatc tgaaccaccc tttattctac atatgatagg
     2881 cagcactgaa atatcctaac cccctaagct ccaggtgccc tgtgggagag caactggact
     2941 atagcagggc tgggctctgt cttcctggtc ataggctcac tctttccccc aaatcttcct
     3001 ctggagcttt gcagccaagg tgctaaaagg aataggtagg agacctcttc tatctaatcc
     3061 ttaaaagcat aatgttgaac attcattcaa cagctgatgc cctataaccc ctgcctggat
     3121 ttcttcctat taggctataa gaagtagcaa gatctttaca taattcagag tggtttcatt
     3181 gccttcctac cctctctaat ggcccctcca tttatttgac taaagcatca cacagtggca
     3241 ctagcattat accaagagta tgagaaatac agtgctttat ggctctaaca ttactgcctt
     3301 cagtatcaag gctgcctgga gaaaggatgg cagcctcagg gcttccttat gtcctccacc
     3361 acaagagctc cttgatgaag gtcatctttt tcccctatcc tgttcttccc ctccccgctc
     3421 ctaatggtac gtgggtaccc aggctggttc ttgggctagg tagtggggac caagttcatt
     3481 acctccctat cagttctagc atagtaaact acggtaccag tgttagtggg aagagctggg
     3541 ttttcctagt atacccactg catcctactc ctacctggtc aacccgctgc ttccaggtat
     3601 gggacctgct aagtgtggaa ttacctgata agggagaggg aaatacaagg agggcctctg
     3661 gtgttcctgg cctcagccag ctgcccacaa gccataaacc aataaaacaa gaatactgag
     3721 tcagtttttt atctgggttc tcttcattcc cactgcactt ggtgctgctt tggctgactg
     3781 ggaacacccc ataactacag agtctgacag gaagactgga gactgtccac ttctagctcg
     3841 gaacttactg tgtaaataaa ctttcagaac tgctaccatg aagtgaaaat gccacatttt
     3901 gctttataat ttctacccat gttgggaaaa actggctttt tcccagccct ttccagggca
     3961 taaaactcaa ccccttcgat agcaagtccc atcagcctat tattttttta aagaaaactt
     4021 gcacttgttt ttctttttac agttacttcc ttcctgcccc aaaattataa actctaagtg
     4081 taaaaaaaag tcttaacaac agcttcttgc ttgtaaaaat atgtattata catctgtatt
     4141 tttaaattct gctcctgaaa aatgactgtc ccattctcca ctcactgcat ttggggcctt
     4201 tcccattggt ctgcatgtct tttatcattg caggccagtg gacagaggga gaagggagaa
     4261 caggggtcgc caacacttgt gttgctttct gactgatcct gaacaagaaa gagtaacact
     4321 gaggcgctcg ctcccatgca caactctcca aaacacttat cctcctgcaa gagtgggctt
     4381 tccagggtct ttactgggaa gcagttaagc cccctcctca ccccttcctt ttttctttct
     4441 ttactccttt ggcttcaaag gattttggaa aagaaacaat atgctttaca ctcattttca
     4501 atttctaaat ttgcagggga tactgaaaaa tacggcaggt ggcctaaggc tgctgtaaag
     4561 ttgaggggag aggaaatctt aagattacaa gataaaaaac gaatccccta aacaaaaaga
     4621 acaatagaac tggtcttcca ttttgccacc tttcctgttc atgacagcta ctaacctgga
     4681 gacagtaaca tttcattaac caaagaaagt gggtcacctg acctctgaag agctgagtac
     4741 tcaggccact ccaatcaccc tacaagatgc caaggaggtc ccaggaagtc cagctcctta
     4801 aactgacgct agtcaataaa cctgggcaag tgaggcaaga gaaatgagga agaatccatc
     4861 tgtgaggtga taggcaagga tgaaagacaa agaaggaaaa gagtatcaaa ggcagaaagg
     4921 agatcattta gttgggtctg aaaggaaaag tctttgctat ccgacatgta ctgctagtac
     4981 ctgtaagcat tttaggtccc agaatggaaa aaaaaatcag ctattggtaa tataataatg
     5041 tcctttccct ggagtcagtt tttttaaaaa gttaactctt agtttttact tgtttaattc
     5101 taaaagagaa gggagctgag gccattccct gtaggagtaa agataaaagg ataggaaaag
     5161 attcaaagct ctaatagagt cacagctttc ccaggtataa aacctaaaat taagaagtac
     5221 aataagcaga ggtggaaaat gatctagttc ctgatagcta cccacagagc aagtgattta
     5281 taaatttgaa atccaaacta ctttcttaat atcactttgg tctccatttt tcccaggaca
     5341 ggaaatatgt ccccccctaa ctttcttgct tcaaaaatta aaatccagca tcccaagatc
     5401 attctacaag taattttgca cagacatctc ctcaccccag tgcctgtctg gagctcaccc
     5461 aaggtcacca aacaacttgg ttgtgaacca actgccttaa ccttctgggg gagggggatt
     5521 agctagacta ggagaccaga agtgaatggg aaagggtgag gacttcacaa tgttggcctg
     5581 tcagagcttg attagaagcc aagacagtgg cagcaaagga agacttggcc caggaaaaac
     5641 ctgtgggttg tgctaatttc tgtccagaaa atagggtgga cagaagcttg tggggtacat
     5701 ggaggaattg ggacctggtt atgttgttat tctcggactg tgaattttgg tgatgtaaaa
     5761 cagaatattc tgtaaaccta atgtctgtat aaataatgag cgttaacaca gtaaaatatt
     5821 caataagaag tcaaa
//
