LOCUS       NM_003779               1933 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens beta-1,4-galactosyltransferase 3 (B4GALT3), transcript
            variant 2, mRNA.
ACCESSION   NM_003779
VERSION     NM_003779.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Bi,C., Shan,J., Li,M., Zhang,Q., Li,C., Tong,J. and Huang,Q.
  TITLE     Long noncoding RNA differentiation antagonizing nonprotein coding
            RNA promotes the proliferation, invasion and migration of
            neuroblastoma cells via targeting beta-1, 4-galactosyltransferase
            III by sponging miR-338-3p
  JOURNAL   Neuroreport 32 (12), 965-974 (2021)
   PUBMED   34050113
  REMARK    GeneRIF: Long noncoding RNA differentiation antagonizing nonprotein
            coding RNA promotes the proliferation, invasion and migration of
            neuroblastoma cells via targeting beta-1, 4-galactosyltransferase
            III by sponging miR-338-3p.
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Wu,T., Li,Y. and Chen,B.
  TITLE     B4GALT3 promotes cell proliferation and invasion in glioblastoma
  JOURNAL   Neurol Res 42 (6), 463-470 (2020)
   PUBMED   32202233
  REMARK    GeneRIF: B4GALT3 promotes cell proliferation and invasion in
            glioblastoma.
REFERENCE   3  (bases 1 to 1933)
  AUTHORS   Tange,R., Tomatsu,T. and Sato,T.
  TITLE     Transcription of human beta4-galactosyltransferase 3 is regulated
            by differential DNA binding of Sp1/Sp3 in SH-SY5Y human
            neuroblastoma and A549 human lung cancer cell lines
  JOURNAL   Glycobiology 29 (3), 211-221 (2019)
   PUBMED   30561605
  REMARK    GeneRIF: These findings suggest that the transcription of the
            b4GalT3 gene is regulated by differential DNA binding of Sp3 and
            Sp1 in neuroblastoma and lung cancer. The increased expression of
            b4GalT3 in neuroblastoma may be ascribed to the enhanced expression
            of Sp3, which is observed for various cancers.
REFERENCE   4  (bases 1 to 1933)
  AUTHORS   Liu,H., Chen,D., Bi,J., Han,J., Yang,M., Dong,W., Lin,T. and
            Huang,J.
  TITLE     Circular RNA circUBXN7 represses cell growth and invasion by
            sponging miR-1247-3p to enhance B4GALT3 expression in bladder
            cancer
  JOURNAL   Aging (Albany NY) 10 (10), 2606-2623 (2018)
   PUBMED   30312173
  REMARK    GeneRIF: In bladder tumor cells circUBXN7 serves as a competitive
            endogenous RNA of miR-1247-3p to elevate B4GALT3 expression,
            consequently inhibiting cell viability and invasion.
REFERENCE   5  (bases 1 to 1933)
  AUTHORS   Fang,T., Lv,H., Lv,G., Li,T., Wang,C., Han,Q., Yu,L., Su,B.,
            Guo,L., Huang,S., Cao,D., Tang,L., Tang,S., Wu,M., Yang,W. and
            Wang,H.
  TITLE     Tumor-derived exosomal miR-1247-3p induces cancer-associated
            fibroblast activation to foster lung metastasis of liver cancer
  JOURNAL   Nat Commun 9 (1), 191 (2018)
   PUBMED   29335551
  REMARK    GeneRIF: High-metastatic hepatocellular carcinoma cells secrete
            exosomal miR-1247-3p that directly targets B4GALT3, leading to
            activation of beta1-integrin-NF-kappaappaB metastatic signaling in
            fibroblasts resulting in lung cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1933)
  AUTHORS   Davidsson,J., Andersson,A., Paulsson,K., Heidenblad,M.,
            Isaksson,M., Borg,A., Heldrup,J., Behrendtz,M., Panagopoulos,I.,
            Fioretos,T. and Johansson,B.
  TITLE     Tiling resolution array comparative genomic hybridization,
            expression and methylation analyses of dup(1q) in Burkitt lymphomas
            and pediatric high hyperdiploid acute lymphoblastic leukemias
            reveal clustered near-centromeric breakpoints and overexpression of
            genes in 1q22-32.3
  JOURNAL   Hum Mol Genet 16 (18), 2215-2225 (2007)
   PUBMED   17613536
  REMARK    GeneRIF: B4GALT3, DAP3, RGS16, TMEM183A and UCK2--were
            significantly overexpressed in dup(1q)-positive ALLs compared with
            high hyperdiploid ALLs without dup(1q).
REFERENCE   7  (bases 1 to 1933)
  AUTHORS   Guo,S., Sato,T., Shirane,K. and Furukawa,K.
  TITLE     Galactosylation of N-linked oligosaccharides by human
            beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed
            in Sf-9 cells
  JOURNAL   Glycobiology 11 (10), 813-820 (2001)
   PUBMED   11588157
REFERENCE   8  (bases 1 to 1933)
  AUTHORS   Amado,M., Almeida,R., Schwientek,T. and Clausen,H.
  TITLE     Identification and characterization of large galactosyltransferase
            gene families: galactosyltransferases for all functions
  JOURNAL   Biochim Biophys Acta 1473 (1), 35-53 (1999)
   PUBMED   10580128
  REMARK    Review article
REFERENCE   9  (bases 1 to 1933)
  AUTHORS   Lo,N.W., Shaper,J.H., Pevsner,J. and Shaper,N.L.
  TITLE     The expanding beta 4-galactosyltransferase gene family: messages
            from the databanks
  JOURNAL   Glycobiology 8 (5), 517-526 (1998)
   PUBMED   9597550
REFERENCE   10 (bases 1 to 1933)
  AUTHORS   Almeida,R., Amado,M., David,L., Levery,S.B., Holmes,E.H., Merkx,G.,
            van Kessel,A.G., Rygaard,E., Hassan,H., Bennett,E. and Clausen,H.
  TITLE     A family of human beta4-galactosyltransferases. Cloning and
            expression of two novel UDP-galactose:beta-n-acetylglucosamine
            beta1, 4-galactosyltransferases, beta4Gal-T2 and beta4Gal-T3
  JOURNAL   J Biol Chem 272 (51), 31979-31991 (1997)
   PUBMED   9405390
  REMARK    Erratum:[J Biol Chem 1998 Jul 17;273(29):18674]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC339671.1, AK291455.1 and
            BC006099.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_003779.3.
            
            Summary: This gene is one of seven beta-1,4-galactosyltransferase
            (beta4GalT) genes. They encode type II membrane-bound glycoproteins
            that appear to have exclusive specificity for the donor substrate
            UDP-galactose; all transfer galactose in a beta1,4 linkage to
            similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a
            distinct function in the biosynthesis of different glycoconjugates
            and saccharide structures. As type II membrane proteins, they have
            an N-terminal hydrophobic signal sequence that directs the protein
            to the Golgi apparatus and which then remains uncleaved to function
            as a transmembrane anchor. By sequence similarity, the beta4GalTs
            form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and
            beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene
            encodes an enzyme that may be mainly involved in the synthesis of
            the first N-acetyllactosamine unit of poly-N-acetyllactosamine
            chains. Multiple alternatively spliced transcript variants encoding
            the same protein have been found for this gene. [provided by
            RefSeq, Dec 2010].
            
            Transcript Variant: This variant (2) lacks an internal segment in
            the 5' UTR, and encodes the same protein, as compared to variant 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189655.181823.1,
                                           SRR3476690.701043.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319769.10/ ENSP00000320965.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 DC339671.1         544-551
            9-22                AK291455.1         3-16
            23-1933             BC006099.1         1-1911
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.3"
     gene            1..1933
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /note="beta-1,4-galactosyltransferase 3"
                     /db_xref="GeneID:8703"
                     /db_xref="HGNC:HGNC:926"
                     /db_xref="MIM:604014"
     exon            1..85
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     exon            86..231
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     exon            232..498
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     CDS             246..1427
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /EC_number="2.4.1.90"
                     /EC_number="2.4.1.38"
                     /EC_number="2.4.1.275"
                     /note="beta-N-acetylglucosaminyl-glycolipid
                     beta-1,4-galactosyltransferase 3; b4Gal-T3;
                     beta-1,4-GalTase 3; epididymis secretory sperm binding
                     protein; nal synthase; N-acetyllactosamine synthase;
                     UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3;
                     UDP-galactose:beta-N-acetylglucosamine
                     beta-1,4-galactosyltransferase 3; UDP-Gal:betaGlcNAc beta
                     1,4- galactosyltransferase, polypeptide 3;
                     beta-N-acetylglucosaminylglycopeptide
                     beta-1,4-galactosyltransferase; neolactotriaosylceramide
                     beta-1,4-galactosyltransferase"
                     /codon_start=1
                     /product="beta-1,4-galactosyltransferase 3"
                     /protein_id="NP_003770.1"
                     /db_xref="CCDS:CCDS1222.1"
                     /db_xref="GeneID:8703"
                     /db_xref="HGNC:HGNC:926"
                     /db_xref="MIM:604014"
                     /translation="MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGP
                     TFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIV
                     ERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYV
                     IHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVA
                     VAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKI
                     SRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPL
                     YTNITADIGTDPRGPRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPLSTANHTALRGS
                     H"
     exon            499..734
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     exon            735..925
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1048
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1153
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1933
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1913..1918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /note="hexamer: AATAAA"
     polyA_site      1933
                     /gene="B4GALT3"
                     /gene_synonym="beta4Gal-T3"
                     /note="major polyA site"
ORIGIN      
        1 ccccgcagcg cccgcttcca agatggcggc agcgatgcct gcccggctgt tggggtggcg
       61 gtgacgacag gcagcaaaag accagctggt cccagattcg ctgctggagt gctggatgga
      121 gcctttctct gccctctgtg acatttccaa ttttagataa tgcctcacat ctctgtcccc
      181 ccgggacccc ctggagcccc catgatccct aagaagacag cttgaaccta gatctcaccc
      241 ccaggatgtt gcggaggctg ctggagcggc cttgcacgct ggccctgctt gtgggctccc
      301 agctggctgt catgatgtac ctgtcactgg ggggcttccg aagtctcagt gccctatttg
      361 gccgagatca gggaccgaca tttgactatt ctcaccctcg tgatgtctac agtaacctca
      421 gtcacctgcc tggggcccca gggggtcctc cagctcctca aggtctgccc tactgtccag
      481 aacgatctcc tctcttagtg ggtcctgtgt cggtgtcctt tagcccagtg ccatcactgg
      541 cagagattgt ggagcggaat ccccgggtag aaccaggggg ccggtaccgc cctgcaggtt
      601 gtgagccccg ctcccgaaca gccatcattg tgcctcatcg tgcccgggag caccacctgc
      661 gcctgctgct ctaccacctg caccccttct tgcagcgcca gcagcttgct tatggcatct
      721 atgtcatcca ccaggctgga aatggaacat ttaacagggc aaaactgttg aacgttgggg
      781 tgcgagaggc cctgcgtgat gaagagtggg actgcctgtt cttgcacgat gtggacctct
      841 tgccagaaaa tgaccacaat ctgtatgtgt gtgacccccg gggaccccgc catgttgccg
      901 ttgctatgaa caagtttgga tacagcctcc cgtaccccca gtacttcgga ggagtctcag
      961 cacttactcc tgaccagtac ctgaagatga atggcttccc caatgaatac tggggctggg
     1021 gtggtgagga tgacgacatt gctaccaggg tgcgcctggc tgggatgaag atctctcggc
     1081 cccccacatc tgtaggacac tataagatgg tgaagcaccg aggagataag ggcaatgagg
     1141 aaaatcccca cagatttgac ctcctggtcc gtacccagaa ttcctggacg caagatggga
     1201 tgaactcact gacataccag ttgctggctc gagagctggg gcctctttat accaacatca
     1261 cagcagacat tgggactgac cctcggggtc ctcgggctcc ttctgggcca cgttacccac
     1321 ctggttcctc ccaagccttc cgtcaagaga tgctgcaacg ccggccccca gccaggcctg
     1381 ggcctctatc tactgccaac cacacagccc tccgaggttc acactgactc ctccttcctg
     1441 tctaccttaa tcatgaaacc gaattcatgg ggttgtattc tccccaccct cagctcctca
     1501 ctgttctcag agggatgtga gggaactgaa ctctggtgcc gtgctagggg gtaggggcct
     1561 ctccctcact gctggactgg agctgggctc ctgtagacct gaggggtccc tctctctagg
     1621 gtctcctgta gggcttatga ctgtgaatcc ttgatgtcat gattttatgt gacgattcct
     1681 aggagtccct gcccctagag taggagcagg gctggacccc aagcccctcc ctcttccatg
     1741 gagagaagag tgatctggct tctcctcgga cctctgtgaa tatttattct atttatggtt
     1801 cccgggaagt tgtttggtga aggaagcccc tccctgggca ttttctgcct atgctggaat
     1861 agctccctct tctggtcctg gctcaggggg ctgggatttt gatatatttt ctaataaagg
     1921 actttgtctc gca
//
