LOCUS       NM_178537               3750 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 4
            (B4GALNT4), mRNA.
ACCESSION   NM_178537 XM_001129003
VERSION     NM_178537.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Baba,H., Kanda,M., Sato,Y., Sawaki,K., Shimizu,D., Koike,M.,
            Motoyama,S., Kodera,Y. and Fujii,T.
  TITLE     Expression and Malignant Potential of B4GALNT4 in Esophageal
            Squamous Cell Carcinoma
  JOURNAL   Ann Surg Oncol 27 (9), 3247-3256 (2020)
   PUBMED   32253672
  REMARK    GeneRIF: Expression and Malignant Potential of B4GALNT4 in
            Esophageal Squamous Cell Carcinoma.
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Hirano,K., Matsuda,A., Kuji,R., Nakandakari,S., Shirai,T. and
            Furukawa,K.
  TITLE     Enhanced expression of the beta4-N-acetylgalactosaminyltransferase
            4 gene impairs tumor growth of human breast cancer cells
  JOURNAL   Biochem Biophys Res Commun 461 (1), 80-85 (2015)
   PUBMED   25858323
  REMARK    GeneRIF: These results indicate that the expression of the LacdiNAc
            group is quite important for the suppression of malignancies of the
            MDA-MB-231 cells.
REFERENCE   3  (bases 1 to 3750)
  AUTHORS   Fiete,D., Beranek,M. and Baenziger,J.U.
  TITLE     Peptide-specific transfer of N-acetylgalactosamine to O-linked
            glycans by the glycosyltransferases beta1,4-N-acetylgalactosaminyl
            transferase 3 (beta4GalNAc-T3) and beta4GalNAc-T4
  JOURNAL   J Biol Chem 287 (34), 29204-29212 (2012)
   PUBMED   22722940
  REMARK    GeneRIF: Peptide-specific transfer of N-acetylgalactosamine to
            O-linked glycans by the glycosyltransferases
            beta1,4-N-acetylgalactosaminyl transferase 3 (beta4GalNAc-T3) and
            beta4GalNAc-T4.
REFERENCE   4  (bases 1 to 3750)
  AUTHORS   Fiete,D., Beranek,M. and Baenziger,J.U.
  TITLE     Molecular basis for protein-specific transfer of
            N-acetylgalactosamine to N-linked glycans by the
            glycosyltransferases beta1,4-N-acetylgalactosaminyl transferase 3
            (beta4GalNAc-T3) and beta4GalNAc-T4
  JOURNAL   J Biol Chem 287 (34), 29194-29203 (2012)
   PUBMED   22722937
  REMARK    GeneRIF: Molecular basis for protein-specific transfer of
            N-acetylgalactosamine to N-linked glycans by the
            glycosyltransferases beta1,4-N-acetylgalactosaminyl transferase 3
            (beta4GalNAc-T3) and beta4GalNAc-T4.
REFERENCE   5  (bases 1 to 3750)
  AUTHORS   Miller,E., Fiete,D., Blake,N.M., Beranek,M., Oates,E.L., Mi,Y.,
            Roseman,D.S. and Baenziger,J.U.
  TITLE     A necessary and sufficient determinant for protein-selective
            glycosylation in vivo
  JOURNAL   J Biol Chem 283 (4), 1985-1991 (2008)
   PUBMED   18048353
  REMARK    GeneRIF: betaGT3 and betaGT4, that are able to transfer GalNAc to
            GlcNAc in beta1,4-linkage display the necessary glycoprotein
            specificity in vivo.
REFERENCE   6  (bases 1 to 3750)
  AUTHORS   Gotoh,M., Sato,T., Kiyohara,K., Kameyama,A., Kikuchi,N., Kwon,Y.D.,
            Ishizuka,Y., Iwai,T., Nakanishi,H. and Narimatsu,H.
  TITLE     Molecular cloning and characterization of
            beta1,4-N-acetylgalactosaminyltransferases IV synthesizing
            N,N'-diacetyllactosediamine
  JOURNAL   FEBS Lett 562 (1-3), 134-140 (2004)
   PUBMED   15044014
  REMARK    GeneRIF: molecular cloning and characterization; specificity toward
            oligosaccharide acceptor substrates was quite similar to
            beta4GalNAc-T3 in vitro but the tissue distributions of the two
            enzymes were quite different
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138230.5, BF058197.1, AB089939.1 and CN348077.1.
            
            On Nov 22, 2018 this sequence version replaced NM_178537.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB089939.1, ERR3218376.233850.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2145893
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000329962.11/ ENSP00000328277.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-296               AC138230.5         86300-86595
            297-305             BF058197.1         1-9
            306-3425            AB089939.1         1-3120
            3426-3750           CN348077.1         113-437
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.5"
     gene            1..3750
                     /gene="B4GALNT4"
                     /note="beta-1,4-N-acetyl-galactosaminyltransferase 4"
                     /db_xref="GeneID:338707"
                     /db_xref="HGNC:HGNC:26315"
                     /db_xref="MIM:618560"
     exon            1..456
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             306..3425
                     /gene="B4GALNT4"
                     /EC_number="2.4.1.244"
                     /note="beta1,4-N-acetylgalactosaminyltransferases IV;
                     beta4GalNAc-T4; NGalNAc-T1; betaGT4;
                     beta-1,4-N-acetyl-galactosaminyl transferase 4;
                     beta4GalNAcT4; beta-1,4-N-acetylgalactosaminyltransferase
                     IV; N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-
                     acetylgalactosaminyltransferase"
                     /codon_start=1
                     /product="N-acetyl-beta-glucosaminyl-glycoprotein
                     4-beta-N-acetylgalactosaminyltransferase 1"
                     /protein_id="NP_848632.2"
                     /db_xref="CCDS:CCDS7694.1"
                     /db_xref="GeneID:338707"
                     /db_xref="HGNC:HGNC:26315"
                     /db_xref="MIM:618560"
                     /translation="MPRLPVKKIRKQMKLLLLLLLLSCAAWLTYVHLGLVRQGRALRQ
                     RLGYGRDGEKLTSETDGRGVHAAPSTQRAEDSSESREEEQAPEGRDLDMLFPGGAGRL
                     PLNFTHQTPPWREEYKGQVNLHVFEDWCGGAVGHLRRNLHFPLFPHTRTTVKKLAVSP
                     KWKNYGLRIFGFIHPARDGDVQFSVASDDNSEFWLSLDESPAAAQLVAFVGKTGSEWT
                     APGEFTKFSSQVSKPRRLMASRRYYFELLHKQDDRGSDHVEVGWRAFLPGLKFEVISS
                     AHISLYTDESALKMDHVAHVPQSPASHVGGRPPQEETSADMLRPDPRDTFFLTPRMES
                     SSLENVLEPCAYAPTYVVKDFPIARYQGLQFVYLSFVYPNDYTRLTHMETDNKCFYRE
                     SPLYLERFGFYKYMKMDKEEGDEDEEDEVQRRAFLFLNPDDFLDDEDEGELLDSLEPT
                     EAAPPRSGPQSPAPAAPAQPGATLAPPTPPRPRDGGTPRHSRALSWAARAARPLPLFL
                     GRAPPPRPAVEQPPPKVYVTRVRPGQRASPRAPAPRAPWPPFPGVFLHPRPLPRVQLR
                     APPRPPRPHGRRTGGPQATQPRPPARAQATQGGREGQARTLGPAAPTVDSNLSSEARP
                     VTSFLSLSQVSGPQLPGEGEEEEEGEDDGAPGDEAASEDSEEAAGPALGRWREDAIDW
                     QRTFSVGAVDFELLRSDWNDLRCNVSGNLQLPEAEAVDVTAQYMERLNARHGGRFALL
                     RIVNVEKRRDSARGSRFLLELELQERGGGRLRLSEYVFLRLPGARVGDADGESPEPAP
                     AASVRPDGRPELCRPLRLAWRQDVMVHFIVPVKNQARWVAQFLADMAALHARTGDSRF
                     SVVLVDFESEDMDVERALRAARLPRYQYLRRTGNFERSAGLQAGVDAVEDASSIVFLC
                     DLHIHFPPNILDGIRKHCVEGRLAFAPVVMRLSCGSSPRDPHGYWEVNGFGLFGIYKS
                     DFDRVGGMNTEEFRDQWGGEDWELLDRVLQAGLEVERLRLRNFYHHYHSKRGMWSVRS
                     RKGSRTGAS"
     exon            457..560
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            561..653
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            654..749
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            750..840
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            841..941
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1009
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1088
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1155
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1290
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1400
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1501
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1602
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..2509
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2793
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..2947
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3020
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3174
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3175..3301
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     exon            3302..3750
                     /gene="B4GALNT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3726..3731
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT4"
                     /note="hexamer: AATGAA"
     polyA_site      3750
                     /gene="B4GALNT4"
                     /note="major polyA site"
ORIGIN      
        1 gatccgcggt ggcagccgtg gatgctgttc ggtcccgccg ccgccccagg agcgcggagc
       61 cgggagcggc cgggcggggg gcaccgcgag gagccgcccc cgccgcccac cccgggcccg
      121 cggccgaggg cggcctgggg gggtcgcggc cgcacccggt ggccgcgcac ggcggacaaa
      181 ggaccatgcg gggccgcggg cgctgagcgc ggcggggcgg gccggggatg cggcgcgggg
      241 cgggcggggg ccgggggctg cagcggcgcc gctgagcgcg gcctggggcg ggcgcggcgg
      301 ccgcgatgcc gcggctcccg gtgaagaaga tccgtaagca gatgaagctg ctgctgctgc
      361 tgctgctgct gagctgcgcc gcgtggctca cctacgtgca cctgggcctg gtgcgccagg
      421 gacgcgcgct gcgccagcgc ctgggctacg ggcgagatgg tgagaagctg accagtgaga
      481 ccgacggccg gggggtccac gctgcgccat ccacacagag ggctgaggac tccagtgaga
      541 gccgtgaaga ggagcaagcg cccgaaggtc gggacctaga catgctgttt cctggggggg
      601 ctgggaggct gccactgaac ttcacccatc agacaccccc atggcgggag gagtacaagg
      661 ggcaggtgaa cctgcacgtg tttgaggact ggtgtggggg cgccgtgggc cacctgagga
      721 ggaacctgca cttcccgctg ttccctcata cgcgcaccac cgtgaagaag ttggccgtgt
      781 cccccaagtg gaagaactat ggactccgta tttttggttt catccacccg gcgagggacg
      841 gagacgtcca gttttctgtg gcctcagacg acaactcgga gttctggctg agtctggacg
      901 agagccctgc tgctgcccag cttgtggcct ttgtgggcaa gactggctcc gagtggacag
      961 cgcctggaga attcaccaag ttcagctccc aggtgtccaa gcccaggcgg ctcatggcct
     1021 cccggaggta ctactttgag ttgctgcaca agcaggacga ccgcggctcg gaccacgtgg
     1081 aagtgggctg gcgagctttc ctgcccggcc tgaagttcga ggtcatcagc tctgctcaca
     1141 tctccctgta cacagatgag tcagccttga agatggacca cgtggcgcac gtcccccagt
     1201 ctccagccag ccacgtgggg gggcgtccgc cgcaggagga gaccagcgca gacatgctgc
     1261 ggccagatcc cagggatacc tttttcctca ctccacgcat ggaatcttcg agcctggaga
     1321 acgtgctgga gccctgcgcc tacgccccca cctacgtggt caaggacttc ccgatcgcca
     1381 gataccaggg cctgcaattt gtgtacctgt ccttcgttta tcccaacgac tacactcgcc
     1441 tcacccacat ggagacggac aacaagtgct tctaccgcga gtctccgctg tatctggaga
     1501 ggtttgggtt ctataaatac atgaagatgg acaaggagga gggggatgag gatgaagaag
     1561 acgaggtgca gcgccgagcc ttcctcttcc tcaacccgga cgacttcctg gacgacgagg
     1621 acgaggggga gctgctcgac agcctggagc ccaccgaggc ggccccgccc aggagcggcc
     1681 cccagtcccc cgccccagca gcccccgccc agcccggagc caccctcgcc ccgccgaccc
     1741 ctccccgccc ccgggacggg gggaccccca ggcactcccg ggccctgagc tgggccgcca
     1801 gggccgcccg ccctttgccg ctcttcttgg gccgagctcc gcccccgcgc cctgcagtgg
     1861 agcagccgcc cccaaaggtg tacgtgacca gggtgcggcc gggacagcgg gcatcccccc
     1921 gggccccagc gccgcgtgcg ccctggccgc ccttccctgg cgtcttcctg caccccaggc
     1981 ctctgcccag agtgcagctg cgggcgcccc cacgcccacc ccggccccac ggccgcagga
     2041 ccggcggccc ccaggccaca cagccgaggc ccccagcccg ggcgcaggcc acccaagggg
     2101 gccgggaggg ccaggcgcgc acgctgggac ctgcggcgcc cacagtggac tcaaacttgt
     2161 cctccgaagc gcggcccgtg acctccttcc tgagcttgtc ccaggtgtcc gggccgcagc
     2221 tgcccgggga gggcgaagag gaggaggaag gggaggacga tggggccccg ggcgacgagg
     2281 ccgcgtcgga ggacagcgag gaggccgcgg gcccggcgct cggacgctgg cgtgaggacg
     2341 ccatcgactg gcagcgcacg ttcagcgtgg gcgccgtgga cttcgagctg ctgcgctcgg
     2401 actggaacga cctgcgatgc aacgtttcgg ggaacctgca gctgccggag gcggaggccg
     2461 tggacgtgac cgctcagtac atggagcggc tgaacgcgcg ccacggcggg cgcttcgcgc
     2521 ttctgcgcat cgtgaacgtg gagaagcgcc gggactcggc gcgagggagt cgcttcctgc
     2581 tggagctgga gctgcaggag cgcgggggcg gccgcctgcg actgtccgag tacgtcttcc
     2641 tgcggctgcc gggagcccgc gtaggggatg cagacggaga aagtcccgaa cccgctcccg
     2701 ccgcctccgt gcgccccgac ggccgccccg agctctgccg gccactgcgc ctggcctggc
     2761 gccaggacgt gatggttcac ttcatcgtgc cagtgaaaaa ccaggcacgg tgggtggcac
     2821 agttcctggc ggacatggct gcgctgcacg cgcgcaccgg ggactcgcgt ttcagcgtcg
     2881 tcctggtgga tttcgagagc gaggatatgg acgtggagcg ggccctgcgc gccgcgcgcc
     2941 tgccccggta ccagtacctg agacgaaccg ggaacttcga gcgctccgcc gggctgcagg
     3001 cgggagtgga cgcggtagag gacgccagca gcatcgtgtt cctctgcgac ctgcacatcc
     3061 acttcccacc caacatcctg gacggcatcc gcaagcactg cgtggagggc aggctggcct
     3121 tcgcgcccgt ggtcatgcgc ctgagctgcg ggagctcgcc ccgggacccc cacggttact
     3181 gggaggtgaa cggctttggc ctttttggga tctacaagtc ggactttgac cgggttggag
     3241 gaatgaacac ggaggagttc cgagaccagt gggggggtga agactgggag ctcctggaca
     3301 gggtcctgca ggcagggctg gaggtggagc ggctccgact gcggaatttc tatcaccact
     3361 accactccaa gaggggcatg tggagcgtcc gcagcaggaa gggctctcgc acgggggcgt
     3421 cttgaggacg ggcagcccct cccagccccg gtgggagtcc cgaggcagct gctgggggct
     3481 gggctttgag ctcggtcccg agagacccgg cagggctggt cagaggggca cagccaccgc
     3541 ctgtgcctgc ccctctctgg cccactgggc gtcgtgcccc tccccggaga ggcagccttc
     3601 acggcgggtc agggcctggc cttggtcccc actctgcgat gatttctgtg aaattttgct
     3661 gtagcgatga cattgttttc agaatttcca agagttctgt ctgttctgtt ttttattcag
     3721 aatgaaatga aatatttttt ttagttctga
//
