LOCUS       NM_153446               9070 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 2 (SID
            blood group) (B4GALNT2), transcript variant 1, mRNA.
ACCESSION   NM_153446
VERSION     NM_153446.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Cogez,V., Vicogne,D., Schulz,C., Portier,L., Venturi,G., de
            Ruyck,J., Decloquement,M., Lensink,M.F., Brysbaert,G.,
            Dall'Olio,F., Groux-Degroote,S. and Harduin-Lepers,A.
  TITLE     N-Glycan on the Non-Consensus N-X-C Glycosylation Site Impacts
            Activity, Stability, and Localization of the Sda Synthase B4GALNT2
  JOURNAL   Int J Mol Sci 24 (4), 4139 (2023)
   PUBMED   36835549
  REMARK    GeneRIF: N-Glycan on the Non-Consensus N-X-C Glycosylation Site
            Impacts Activity, Stability, and Localization of the Sd[a] Synthase
            B4GALNT2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Stenfelt,L., Nilsson,J., Hellberg,A., Liew,Y.W., Morrison,J.,
            Larson,G. and Olsson,M.L.
  TITLE     Glycoproteomic and Phenotypic Elucidation of B4GALNT2 Expression
            Variants in the SID Histo-Blood Group System
  JOURNAL   Int J Mol Sci 23 (7), 3936 (2022)
   PUBMED   35409292
  REMARK    GeneRIF: Glycoproteomic and Phenotypic Elucidation of B4GALNT2
            Expression Variants in the SID Histo-Blood Group System.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9070)
  AUTHORS   Groux-Degroote,S., Vicogne,D., Cogez,V., Schulz,C. and
            Harduin-Lepers,A.
  TITLE     B4GALNT2 Controls Sda and SLex Antigen Biosynthesis in Healthy and
            Cancer Human Colon
  JOURNAL   Chembiochem 22 (24), 3381-3390 (2021)
   PUBMED   34397142
  REMARK    GeneRIF: B4GALNT2 Controls Sd(a) and SLe(x) Antigen Biosynthesis in
            Healthy and Cancer Human Colon.
            Review article
REFERENCE   4  (bases 1 to 9070)
  AUTHORS   Wavelet-Vermuse,C., Groux-Degroote,S., Vicogne,D., Cogez,V.,
            Venturi,G., Trinchera,M., Brysbaert,G., Krzewinski-Recchi,M.A.,
            Hadj Bachir,E., Schulz,C., Vincent,A., Van Seuningen,I. and
            Harduin-Lepers,A.
  TITLE     Analysis of the proximal promoter of the human colon-specific
            B4GALNT2 (Sda synthase) gene: B4GALNT2 is transcriptionally
            regulated by ETS1
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1864 (11-12), 194747 (2021)
   PUBMED   34500083
  REMARK    GeneRIF: Analysis of the proximal promoter of the human
            colon-specific B4GALNT2 (Sd(a) synthase) gene: B4GALNT2 is
            transcriptionally regulated by ETS1.
REFERENCE   5  (bases 1 to 9070)
  AUTHORS   Pucci,M., Malagolini,N. and Dall'Olio,F.
  TITLE     Glycosyltransferase B4GALNT2 as a Predictor of Good Prognosis in
            Colon Cancer: Lessons from Databases
  JOURNAL   Int J Mol Sci 22 (9), 4331 (2021)
   PUBMED   33919332
  REMARK    GeneRIF: Glycosyltransferase B4GALNT2 as a Predictor of Good
            Prognosis in Colon Cancer: Lessons from Databases.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9070)
  AUTHORS   Lo Presti,L., Cabuy,E., Chiricolo,M. and Dall'Olio,F.
  TITLE     Molecular cloning of the human beta1,4
            N-acetylgalactosaminyltransferase responsible for the biosynthesis
            of the Sd(a) histo-blood group antigen: the sequence predicts a
            very long cytoplasmic domain
  JOURNAL   J Biochem 134 (5), 675-682 (2003)
   PUBMED   14688233
  REMARK    GeneRIF: molecular cloning; cDNA predicts a 566 aa protein showing
            66.6% and 39% identity with mouse CT beta4GalNAc-T and human
            GM2/GD2 synthase
REFERENCE   7  (bases 1 to 9070)
  AUTHORS   Montiel,M.D., Krzewinski-Recchi,M.A., Delannoy,P. and
            Harduin-Lepers,A.
  TITLE     Molecular cloning, gene organization and expression of the human
            UDP-GalNAc:Neu5Acalpha2-3Galbeta-R
            beta1,4-N-acetylgalactosaminyltransferase responsible for the
            biosynthesis of the blood group Sda/Cad antigen: evidence for an
            unusual extended cytoplasmic domain
  JOURNAL   Biochem J 373 (Pt 2), 369-379 (2003)
   PUBMED   12678917
REFERENCE   8  (bases 1 to 9070)
  AUTHORS   Berger,E.G.
  TITLE     Ectopic localizations of Golgi glycosyltransferases
  JOURNAL   Glycobiology 12 (2), 29R-36R (2002)
   PUBMED   11886838
  REMARK    Review article
REFERENCE   9  (bases 1 to 9070)
  AUTHORS   Dohi,T., Yuyama,Y., Natori,Y., Smith,P.L., Lowe,J.B. and Oshima,M.
  TITLE     Detection of N-acetylgalactosaminyltransferase mRNA which
            determines expression of Sda blood group carbohydrate structure in
            human gastrointestinal mucosa and cancer
  JOURNAL   Int J Cancer 67 (5), 626-631 (1996)
   PUBMED   8782649
REFERENCE   10 (bases 1 to 9070)
  AUTHORS   Piller,F., Blanchard,D., Huet,M. and Cartron,J.P.
  TITLE     Identification of a alpha-NeuAc-(2----3)-beta-D-galactopyranosyl
            N-acetyl-beta-D-galactosaminyltransferase in human kidney
  JOURNAL   Carbohydr Res 149 (1), 171-184 (1986)
   PUBMED   2425965
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC069454.22, AJ517770.1, BC113675.1, AJ517771.1 and AK302876.1.
            
            On Jun 2, 2019 this sequence version replaced NM_153446.2.
            
            Summary: B4GALNT2 catalyzes the last step in the biosynthesis of
            the human Sd(a) antigen through the addition of an
            N-acetylgalactosamine residue via a beta-1,4 linkage to a
            subterminal galactose residue substituted with an alpha-2,3-linked
            sialic acid. B4GALNT2 also catalyzes the last step in the
            biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed
            12678917]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ517770.1, AF510036.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142348, SAMEA2145240
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               AC069454.22        22433-22630
            199-298             AJ517770.1         1-100
            299-299             AC069454.22        22731-22731
            300-375             BC113675.1         96-171
            376-376             AC069454.22        22808-22808
            377-1357            BC113675.1         173-1153
            1358-2104           AJ517771.1         942-1688
            2105-2148           AK302876.1         1705-1748
            2149-2149           AC069454.22        59583-59583
            2150-2219           AK302876.1         1750-1819
            2220-9070           AC069454.22        59654-66504
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     gene            1..9070
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="beta-1,4-N-acetyl-galactosaminyltransferase 2 (SID
                     blood group)"
                     /db_xref="GeneID:124872"
                     /db_xref="HGNC:HGNC:24136"
                     /db_xref="MIM:111730"
     exon            1..451
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..1958
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /EC_number="2.4.1.-"
                     /note="isoform a is encoded by transcript variant 1;
                     UDP-GalNAc:Neu5Acalpha2-3Galbeta-R
                     beta1,4-N-acetylgalactosaminyltransferase; sda
                     beta-1,4-GalNAc transferase; beta-1,4
                     N-acetylgalactosaminyltransferase 2;
                     beta-4-N-acetylgalactosaminyltransferase; beta 1,4
                     N-acetylgalactosaminyltransferase/betal,4
                     GalNAcT/Sda-GalNAcT; sd(a) beta-1,4-GalNAc transferase;
                     UDP-GalNAc:Neu5Aca2-3Galb-R
                     b1,4-N-acetylgalactosaminyltransferase;
                     beta-1,4-N-acetyl-galactosaminyl transferase 2"
                     /codon_start=1
                     /product="beta-1,4 N-acetylgalactosaminyltransferase 2
                     isoform a"
                     /protein_id="NP_703147.2"
                     /db_xref="CCDS:CCDS11544.1"
                     /db_xref="GeneID:124872"
                     /db_xref="HGNC:HGNC:24136"
                     /db_xref="MIM:111730"
                     /translation="MGSAGFSVGKFHVEVASRGRECVSGTPECGNRLGSAGFGALCLE
                     LRGADPAWGPFAAHGRSRRQGSRFLWLLKILVIILVLGIVGFMFGSMFLQAVFSSPKP
                     ELPSPAPGVQKLKLLPEERLRNLFSYDGIWLFPKNQCKCEANKEQGGYNFQDAYGQSD
                     LPAVKARRQAEFEHFQRREGLPRPLPLLVQPNLPFGYPVHGVEVMPLHTVPIPGLQFE
                     GPDAPVYEVTLTASLGTLNTLADVPDSVVQGRGQKQLIISTSDRKLLKFILQHVTYTS
                     TGYQHQKVDIVSLESRSSVAKFPVTIRHPVIPKLYDPGPERKLRNLVTIATKTFLRPH
                     KLMIMLRSIREYYPDLTVIVADDSQKPLEIKDNHVEYYTMPFGKGWFAGRNLAISQVT
                     TKYVLWVDDDFLFNEETKIEVLVDVLEKTELDVVGGSVLGNVFQFKLLLEQSENGACL
                     HKRMGFFQPLDGFPSCVVTSGVVNFFLAHTERLQRVGFDPRLQRVAHSEFFIDGLGTL
                     LVGSCPEVIIGHQSRSPVVDSELAALEKTYNTYRSNTLTRVQFKLALHYFKNHLQCAA
                     "
     exon            452..652
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            653..790
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            791..897
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            898..935
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1116
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1203
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1391
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1532
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1752
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..9070
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4306..4311
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: ATTAAA"
     polyA_site      4324
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="major polyA site"
     regulatory      5440..5445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: AATAAA"
     polyA_site      5471
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="major polyA site"
     regulatory      6837..6842
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: TATAAA"
     polyA_site      6866
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
     regulatory      7631..7636
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: AATATA"
     polyA_site      7650
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
     regulatory      9036..9041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: AATAAA"
     polyA_site      9070
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
ORIGIN      
        1 agtctgaggg cggcgggatt cgggatgact tcgggcgggt gagtgtcccc ggggcagagc
       61 agagcgagag gtgaaacttc gggagcaggg agcgccgcgg gtcctttctg gcgtctgcag
      121 agcgggcagg tgctggggac gcagacgccg gagccaggga gcgggcggtt ggagtcttaa
      181 gtccaaccgg ttccccgcat aggtggctgc agaggcgagg tgacggcgcg tgcggaacga
      241 actctgcacc cccaggaatg gggagcgctg gcttttccgt gggaaaattc cacgtggaag
      301 tggcctctcg cggccgggaa tgtgtctcgg ggacgcccga gtgtgggaat cggctcggga
      361 gtgcgggctt cggggctctc tgcttggaac tcagaggcgc tgacccagcc tggggcccgt
      421 ttgctgccca cgggaggagc cgccgtcagg gctcgagatt tctgtggctc ctcaagatat
      481 tggtcataat cctggtactt ggcattgttg gatttatgtt cggaagcatg ttccttcaag
      541 cagtgttcag cagccccaag ccagaactcc caagtcctgc cccgggtgtc cagaagctga
      601 agcttctgcc tgaggaacgt ctcaggaacc tcttttccta cgatggaatc tggctgttcc
      661 cgaaaaatca gtgcaaatgt gaagccaaca aagagcaggg aggttacaac tttcaggatg
      721 cctatggcca gagcgacctc ccagcggtga aagcgaggag acaggctgaa tttgaacact
      781 ttcagaggag agaagggctg ccccgcccac tgcccctgct ggtccagccc aacctcccct
      841 ttgggtaccc agtccacgga gtggaggtga tgcccctgca cacggttccc atcccaggcc
      901 tccagtttga aggacccgat gcccccgtct atgaggtcac cctgacagct tctctgggga
      961 cactgaacac ccttgctgat gtcccagaca gtgtggtgca gggcagaggc cagaagcagc
     1021 tgatcatttc taccagtgac cggaagctgt tgaagttcat tcttcagcac gtgacataca
     1081 ccagcacggg gtaccagcac cagaaggtag acatagtgag tctggagtcc aggtcctcag
     1141 tggccaagtt tccagtgacc atccgccatc ctgtcatacc caagctatac gaccctggac
     1201 cagagaggaa gctcagaaac ctggttacca ttgctaccaa gactttcctc cgcccccaca
     1261 agctcatgat catgctccgg agtattcgag agtattaccc agacttgacc gtaatagtgg
     1321 ctgatgacag ccagaagccc ctggaaatta aagacaatca cgtggagtat tacactatgc
     1381 cctttgggaa gggttggttt gctggtagga acctggccat atctcaggtc accaccaaat
     1441 acgttctctg ggtggacgat gattttctct tcaacgagga gaccaagatt gaggtgctgg
     1501 tggatgtcct ggagaaaaca gaactggacg tggtaggcgg cagtgtgctg ggaaatgtgt
     1561 tccagtttaa gttgttgctg gaacagagtg agaatggggc ctgccttcac aagaggatgg
     1621 gatttttcca acccctggat ggcttcccca gctgcgtggt gaccagtggc gtggtcaact
     1681 tcttcctggc ccacacggag cgactccaaa gagttggctt tgatccccgc ctgcaacgag
     1741 tggctcactc agaattcttc attgatgggc tagggaccct actcgtgggg tcatgcccag
     1801 aagtgattat aggtcaccag tctcggtctc cagtggtgga ctcagaactg gctgccctag
     1861 agaagaccta caatacatac cggtccaaca ccctcacccg ggtccagttc aagctggccc
     1921 tccactactt caagaaccat ctccaatgtg ccgcataaag gtgtgagggc ataggagaaa
     1981 cactaggctg gctggttatg gtatctatag caggccacca aaaactggac tcctgatagg
     2041 tgaacgttgt accaaaccag ctggtgggta gggaaaaggg aaatggctca gttactggaa
     2101 gtaccaatca aaggtgaagg gtcactggaa atgaaccagt cactgaccag ggcaatggag
     2161 actgtattaa tagcaatgat gatttgtaca atgccctgcc ttttttgaag catttgcatg
     2221 ggcagtatct cacatcatct catctgatat cacacaaaga tgacattggt tggtgtcctc
     2281 agtcaaaaga acagctgcct ggtccttgat agttgtttgg tggaaattct gtcacagtca
     2341 gaagcatcgg atccagcatc actgtcatcc ctatattttt attaggttct atcttgtaga
     2401 ctcaaaaaat gtgaacattc cagggtcctt gccttgagga ggtttgcagc ctggttcagt
     2461 tcagtccaat cacagaatta ggcagccggg gtcagaggat cagagaaggg aaggggtcac
     2521 caggtcccag gtggtggagg aagactccgt ggagtgaact tgttaggagc ctgaaagcag
     2581 acattcaggc atcagagagg gtaggagggc cctggggcca tcccactgtc caccagaaca
     2641 aaggctgccc tggcatcaga ctcactccag acctgttcca gaatgccagg gattgaactc
     2701 agagcccatg ccaaggcatg atggtgaaca gacagtgtga gtgtggtgac aagccactaa
     2761 actccaggga cctgaggcca gttgctggtc actcaaaact catgtcattt acagagtggt
     2821 tccatcactc tgttgttgga taagcctctt tctgttgcct gggggttggc agattgccga
     2881 gaccagcttg gtcatggaga ccctaaccca gtggtgctag aggaattaaa gacacacaca
     2941 tagaaatata gagtgtggag tgggaaatca ggggactcac agacttcaga gctgagagcc
     3001 ctgaacggag tttgacccac atatttattg acagcaagcc agtgataagc attgtttcta
     3061 tagattatag ataaactaaa agtattcctt acaggaaaca aagggatggg ccgaaacaaa
     3121 gggatgggct ctggctagtt atctgcagca ggaacatgtc cttaaagcac agatagctca
     3181 tactattatt tgtggcttag gaatgcttta agcggttttc caccctgggt gggccaggtg
     3241 ttccttgccc ttattctgat aaacccacaa ccttcagttt gggcatcatg gccatcatga
     3301 acatgtcaca gtactgcaga gattttgttt atggccagtt ttggggccag tttatggcca
     3361 gatttggggg cctgttccca acacgttccc ctttttgttt tccaaagtga taaaagcaaa
     3421 ggcagctttg tcatggtgag ctaattctca caggagtcag gatccgcacc tgcagactat
     3481 acaaagacaa acaacacaga ttaaaagcac aatcatcatt gaaatcacag agcttccaag
     3541 tgtctttatc cactttaatg ggttaatatc tgctaatctg tctgcagctc cttcaagcac
     3601 tccagttcct ggcattaagg tcaagtgtgc ctgggatgct ttaaatattt gttcttttaa
     3661 ttttgcaata tgcaaagaca agtttgtaga gtgtccttct agatgctttt ttattctttc
     3721 ccaaattttg atcttattaa cagacattaa tagtttccac aaatccttat gtttagcttc
     3781 tacagtggac catatcattt gaggttgagg tgccactata ccgccacgtt tccagataat
     3841 gggaactctt gctgtatttc ttaccatttc taccatctga ctgttttgtt tagaccagct
     3901 gaacatagtg tggctgtggc acacagactg agaggtgtaa tttaagctaa acatcccctt
     3961 aggggaccaa tcaataatga ttccatagga atcgttgtgc agcacctcta cctgttctgc
     4021 aatgcaatct tcccaaacaa gtacattcat tatttctggc caggtctaat tctatttaca
     4081 aataggtttt gagctgcctt tgctttgcct caattatagg agcagattta ttatggtaaa
     4141 tattaagagc agaaagcatg tgtaactgtg tcacacagtg attacatcca ggcattattg
     4201 ccagccaaga ttgataaata tgcccaataa gtataactgt tctctgtgtt gttccttgtt
     4261 gaaggaatac tcatggcaat ggtgatcacc actatcatat ctatcattaa attactcatt
     4321 gtgactggtt gtcccacttt cctcaggttt tcttccacca tctgtgacag cttcttgatc
     4381 tgtccccagg taggtggctg tgttcgacag ctgttgctca tgatagttgg ggtcctcctc
     4441 agcatcagtc ttgacatggc tgcaaccagg ggatcctcgg gatcctcctg gaatctcttc
     4501 ctcagcatct ggctcgtggc aaggtttcag gtgtcttgat ggcacccaaa ttggctattg
     4561 attcggtcct ggagaaacac aagcataacc tctaccccaa gttattattt tacctatttc
     4621 ccaacttttt gttattgtat ctctccacca aaccagttgt tctgcttctg tctttgcagc
     4681 tggtttctgt agatgctgtt cagctgctgg cctttaagca ggctcaaaaa atttaaagtc
     4741 aataatgcta gaatcaattg catatggggt gtcccgtagt ccctgcttct ccccttttgc
     4801 ttttgcaact gctgtttcag ggagagattc attctttcta ctatggcttg tccttgagaa
     4861 ttatatggga tgccagtaat gtgtttaata ttccatatag agaaaaatgt agctagagct
     4921 tggctagtac agcctggggc attatctgtt ttaatagaag ctggaatgcc catcaccgca
     4981 aaacactgca aaaggtgacg tttaacacag gcagaagact ctcctgattg gcatgtagcc
     5041 cagacaaagt gagaaaaggt gtccacacat acatgtacat aagctagtct cccaaacgag
     5101 ggaacatgtg tgacatccat ttgccaaaga gaattaggtt ccaatccttg aggattaact
     5161 cctcctgtaa aagatgagga atgcaccatt tggcaagttg ggcatcgctg gataatagct
     5221 ttagcttctt tccaggtaat gccgtatctg tgtttgagac cagaggcatt aacataggtt
     5281 aaattgtgaa agtgtctagc gttagatatt gcattagcaa ctaggtgatc agccatttga
     5341 ttcccttcag ttaaaggtcc tggaagaggt gtatgagccc taatgtgagt gatgtaaaaa
     5401 ggatgcattc tactcctgac tgctgtttgc aattgggtaa ataaagccat cagttgctca
     5461 tctgtgtgga atcgtagttg agcattttca attaattgtg tggaatgaat catgtatgaa
     5521 gaatcagaaa tcacattgat aggcctatta aaagcagtca atacctcaat tacagctata
     5581 agctctgctt tttgaactga agtatagggc atctgaagcc cttacttttc aagccagagt
     5641 tagaagcttt accattacta gacccatctg tgaagacatt ttcagcacct tcaagtggtt
     5701 tgaatttagt tattttaggg agaatccaat tagttaattt caaaaattga aacaattttg
     5761 ttttgggaaa atgattatca agaataccca caaagtcagc taaatgggtt tgccaggtaa
     5821 gactatttat aaaagcttgc tctatttgtg ccttcatgag aaggacaata atttttccag
     5881 gaccatatcc atgtaattta acaatctgag ttctcccatt tcctatcata gtagcgattt
     5941 gatccaaata aggagttaga gtccgtgaat tagtatgtgg aagaaaaagc cgctctacaa
     6001 gatcttgctc ttgaacaata acaccagtag gtgaatgccg agttgaaaaa attagcaaat
     6061 ctagagtctt ttctggatct attatattta tttgagcttt atggacttgc tttttgatga
     6121 gctgcagctc tgcatcagct tcttttgtta attgccgagg gctatgagac tagcatctcc
     6181 tctaaggtta gaaaatggca taggtaggaa tgcctagagt aggtcatatc caattaatgt
     6241 cccctggtaa tttttgaaag tcatttaatg ttttcagttg atccctacat atggttactt
     6301 tctgtggcac aatgatagtg tcatttactg aagtctccaa gtaggagtaa ggagtagcag
     6361 tctgaatttt gtcaggagct ataattaaac cagcatgaga aatcaaattt tgcaagtgat
     6421 cataacattg gagtaatatt tctcgagtgg ggggcagcac aaagtatatc gtccatataa
     6481 tgaataatgt aacactgtga aaatttttta tgagtaggtt caattgtttg ccctacataa
     6541 gtctggcaaa ttgttggact gtttaacatg ccttgtggca acagtttcca gtgaaaatgc
     6601 ttagcaggct gcaggttgtt tactgcagga attgtaaatg caaaccattc acagtcctgc
     6661 tcagctaagg ggatagtaaa gaaacagtct tttaaatcta tgactattaa aggccaattc
     6721 tttggaatca tagcaggaga aggcagtcct ggctgcaatg tccccatagg ttgtataact
     6781 gaattaatgg ctcttaagtc agttaacagt ctccatgtac ctgatttttt cttaattata
     6841 aaaactggag agttccaagg ggaaaatgtt ggagctatgt gtcctttttc taattgttca
     6901 gtaactaagt cctctgagcc tccagtttct ctttacttag cagtcattgt tctatccaaa
     6961 ttggctcatc tgttaaccat tttaaaggta taggttctgg aagcttaaca atggctgcca
     7021 tcaaaaatga tatcctaaac cttggcagga actttgtctt tccgcttgaa gtggttcctt
     7081 caaatcttgc aaattttttc ctagtcccat accagggaca taccccattt catgcaccat
     7141 acgttgactt tgagggctat acaattgctc tagaattaga acttgtgcta tccattgttg
     7201 taacaaatct ctttcccata aatgtatagg tacagaagtt ataattggtt gaatagtccc
     7261 atgttgtcca tcgggcccct cacaatgcaa aatataatta ctttgatata cttcaggggc
     7321 tttaccaact ccaactatgt taaattgagt gggttgaatt tgccacgtgg acagccagtg
     7381 ctgtagagaa atgattgaaa tgtctgctcc tgtatctacc aaacctttaa atttctttcc
     7441 ctgaatagtt atttcacagg taggatgttt atcagtaatt tgattcaccc aataagctgc
     7501 tttgtcttgt ttatttgtgc ttccaaattc tcctattcgt ttaatttcac ttttccccat
     7561 ttccacatac ggcacaatca gaagttgtgc tatacgcttt cctggctctg ctttccaagg
     7621 aaaagaagta aatataacaa tttgaatttc cccattgtaa tctgaatcaa tgactcctgt
     7681 atgtacttgc actcctttta aatttaaact agatctacct agaagtaatc ctatcatccc
     7741 cccggcaagc atccacagac ccctattgga actttttgtg gggattcccc aagcataagg
     7801 ctcacagctt ttgtgcagta taaatctact gtggcactac tggctgtcgt gggggacaga
     7861 cattgtacag gggtgaggga gtgagctata atatttccct gttgatctgg ggggtatatc
     7921 ctaacaggga actgccaagc ctctatatca ccctctcgtc tagctggctc aattcctgcc
     7981 tgaatagaac tgagagcagt cactcgaggc actgctagaa cagtcactgg ggcaactact
     8041 tttcacccag tgtcctctgg aaaagaaaga tctgtagggt caggtcactc tttttcttca
     8101 aaataatgag ggggtgaaga agagtaggga caaacctctt cctcatttgc cgctttagct
     8161 tcagccagca aacaaacctg ctgtgtcatc tcttctgtta ctttgttata ctttccttcc
     8221 ttctcatcat tagtgtgaaa aggttctaag gtggaacgca ccagagccca cacttgtccc
     8281 attgttaccc tgatgtttcc gagctcccct tcttactcac catggggatt gcttaagggt
     8341 actcgggtgt cctccagctt agttccacat tctccaacca tcactccagc gacccttcaa
     8401 ctggggttca agccccacgt tgggcgccac ttgccgacac cagctcggtc ttggagaccc
     8461 taacccagcg gcgctagagg aattaaagac acacacacag aaatatagag tgtggagtgg
     8521 gaaatcaggg gactcacagc cttcagagct gaaagccccg aacagagttt gactcacatg
     8581 tttattgaca gcaagccagt gataagcatt gtttctaaag attatagatt aactaaaagt
     8641 attccttatg ggaaacaaag ggatggactg aaacaaaggg atgggctctg gctagttatc
     8701 tgccacgtcc ttaaggcaca gctcgctcat gctattgttt gtggcttagg aacaccttaa
     8761 gaggttttcc cccctgggtg ggacaggtgt tccttgccct cattccagta aatccacaac
     8821 cttcagtgtg tgcatcatgg ccatcatgaa catgtcacag tgctgcagag attttattta
     8881 tggccagttt atggccagat ttgggggcct gttcccaaca gcagattcta tgaccacttc
     8941 agccctgcct ttacagtttc tgtttacctg taaacttttt cttcctgtgg cctagggtag
     9001 ttcaggatca ctaaaaatgt gttctgtgat atattaataa aaattcctga aaaatggact
     9061 tacagcaaaa
//
