LOCUS       NM_001159387            8657 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens beta-1,4-N-acetyl-galactosaminyltransferase 2 (SID
            blood group) (B4GALNT2), transcript variant 2, mRNA.
ACCESSION   NM_001159387
VERSION     NM_001159387.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Cogez,V., Vicogne,D., Schulz,C., Portier,L., Venturi,G., de
            Ruyck,J., Decloquement,M., Lensink,M.F., Brysbaert,G.,
            Dall'Olio,F., Groux-Degroote,S. and Harduin-Lepers,A.
  TITLE     N-Glycan on the Non-Consensus N-X-C Glycosylation Site Impacts
            Activity, Stability, and Localization of the Sda Synthase B4GALNT2
  JOURNAL   Int J Mol Sci 24 (4), 4139 (2023)
   PUBMED   36835549
  REMARK    GeneRIF: N-Glycan on the Non-Consensus N-X-C Glycosylation Site
            Impacts Activity, Stability, and Localization of the Sd[a] Synthase
            B4GALNT2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Stenfelt,L., Nilsson,J., Hellberg,A., Liew,Y.W., Morrison,J.,
            Larson,G. and Olsson,M.L.
  TITLE     Glycoproteomic and Phenotypic Elucidation of B4GALNT2 Expression
            Variants in the SID Histo-Blood Group System
  JOURNAL   Int J Mol Sci 23 (7), 3936 (2022)
   PUBMED   35409292
  REMARK    GeneRIF: Glycoproteomic and Phenotypic Elucidation of B4GALNT2
            Expression Variants in the SID Histo-Blood Group System.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8657)
  AUTHORS   Groux-Degroote,S., Vicogne,D., Cogez,V., Schulz,C. and
            Harduin-Lepers,A.
  TITLE     B4GALNT2 Controls Sda and SLex Antigen Biosynthesis in Healthy and
            Cancer Human Colon
  JOURNAL   Chembiochem 22 (24), 3381-3390 (2021)
   PUBMED   34397142
  REMARK    GeneRIF: B4GALNT2 Controls Sd(a) and SLe(x) Antigen Biosynthesis in
            Healthy and Cancer Human Colon.
            Review article
REFERENCE   4  (bases 1 to 8657)
  AUTHORS   Wavelet-Vermuse,C., Groux-Degroote,S., Vicogne,D., Cogez,V.,
            Venturi,G., Trinchera,M., Brysbaert,G., Krzewinski-Recchi,M.A.,
            Hadj Bachir,E., Schulz,C., Vincent,A., Van Seuningen,I. and
            Harduin-Lepers,A.
  TITLE     Analysis of the proximal promoter of the human colon-specific
            B4GALNT2 (Sda synthase) gene: B4GALNT2 is transcriptionally
            regulated by ETS1
  JOURNAL   Biochim Biophys Acta Gene Regul Mech 1864 (11-12), 194747 (2021)
   PUBMED   34500083
  REMARK    GeneRIF: Analysis of the proximal promoter of the human
            colon-specific B4GALNT2 (Sd(a) synthase) gene: B4GALNT2 is
            transcriptionally regulated by ETS1.
REFERENCE   5  (bases 1 to 8657)
  AUTHORS   Pucci,M., Malagolini,N. and Dall'Olio,F.
  TITLE     Glycosyltransferase B4GALNT2 as a Predictor of Good Prognosis in
            Colon Cancer: Lessons from Databases
  JOURNAL   Int J Mol Sci 22 (9), 4331 (2021)
   PUBMED   33919332
  REMARK    GeneRIF: Glycosyltransferase B4GALNT2 as a Predictor of Good
            Prognosis in Colon Cancer: Lessons from Databases.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8657)
  AUTHORS   Lo Presti,L., Cabuy,E., Chiricolo,M. and Dall'Olio,F.
  TITLE     Molecular cloning of the human beta1,4
            N-acetylgalactosaminyltransferase responsible for the biosynthesis
            of the Sd(a) histo-blood group antigen: the sequence predicts a
            very long cytoplasmic domain
  JOURNAL   J Biochem 134 (5), 675-682 (2003)
   PUBMED   14688233
  REMARK    GeneRIF: molecular cloning; cDNA predicts a 566 aa protein showing
            66.6% and 39% identity with mouse CT beta4GalNAc-T and human
            GM2/GD2 synthase
REFERENCE   7  (bases 1 to 8657)
  AUTHORS   Montiel,M.D., Krzewinski-Recchi,M.A., Delannoy,P. and
            Harduin-Lepers,A.
  TITLE     Molecular cloning, gene organization and expression of the human
            UDP-GalNAc:Neu5Acalpha2-3Galbeta-R
            beta1,4-N-acetylgalactosaminyltransferase responsible for the
            biosynthesis of the blood group Sda/Cad antigen: evidence for an
            unusual extended cytoplasmic domain
  JOURNAL   Biochem J 373 (Pt 2), 369-379 (2003)
   PUBMED   12678917
REFERENCE   8  (bases 1 to 8657)
  AUTHORS   Berger,E.G.
  TITLE     Ectopic localizations of Golgi glycosyltransferases
  JOURNAL   Glycobiology 12 (2), 29R-36R (2002)
   PUBMED   11886838
  REMARK    Review article
REFERENCE   9  (bases 1 to 8657)
  AUTHORS   Dohi,T., Yuyama,Y., Natori,Y., Smith,P.L., Lowe,J.B. and Oshima,M.
  TITLE     Detection of N-acetylgalactosaminyltransferase mRNA which
            determines expression of Sda blood group carbohydrate structure in
            human gastrointestinal mucosa and cancer
  JOURNAL   Int J Cancer 67 (5), 626-631 (1996)
   PUBMED   8782649
REFERENCE   10 (bases 1 to 8657)
  AUTHORS   Piller,F., Blanchard,D., Huet,M. and Cartron,J.P.
  TITLE     Identification of a alpha-NeuAc-(2----3)-beta-D-galactopyranosyl
            N-acetyl-beta-D-galactosaminyltransferase in human kidney
  JOURNAL   Carbohydr Res 149 (1), 171-184 (1986)
   PUBMED   2425965
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC069454.22, AJ517771.1 and AK302876.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001159387.1.
            
            Summary: B4GALNT2 catalyzes the last step in the biosynthesis of
            the human Sd(a) antigen through the addition of an
            N-acetylgalactosamine residue via a beta-1,4 linkage to a
            subterminal galactose residue substituted with an alpha-2,3-linked
            sialic acid. B4GALNT2 also catalyzes the last step in the
            biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed
            12678917]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, lacks a
            portion of the 5' coding region, and initiates translation at an
            alternate start codon, compared to variant 1. The encoded isoform
            (b) has a distinct N-terminus and is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ517771.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA2142348
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000393354.7/ ENSP00000377022.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AC069454.22        22433-22448
            17-1691             AJ517771.1         14-1688
            1692-1735           AK302876.1         1705-1748
            1736-1736           AC069454.22        59583-59583
            1737-1806           AK302876.1         1750-1819
            1807-8657           AC069454.22        59654-66504
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     gene            1..8657
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="beta-1,4-N-acetyl-galactosaminyltransferase 2 (SID
                     blood group)"
                     /db_xref="GeneID:124872"
                     /db_xref="HGNC:HGNC:24136"
                     /db_xref="MIM:111730"
     exon            1..38
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1545
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /EC_number="2.4.1.-"
                     /note="isoform b is encoded by transcript variant 2;
                     UDP-GalNAc:Neu5Acalpha2-3Galbeta-R
                     beta1,4-N-acetylgalactosaminyltransferase; sda
                     beta-1,4-GalNAc transferase; beta-1,4
                     N-acetylgalactosaminyltransferase 2;
                     beta-4-N-acetylgalactosaminyltransferase; beta 1,4
                     N-acetylgalactosaminyltransferase/betal,4
                     GalNAcT/Sda-GalNAcT; sd(a) beta-1,4-GalNAc transferase;
                     UDP-GalNAc:Neu5Aca2-3Galb-R
                     b1,4-N-acetylgalactosaminyltransferase;
                     beta-1,4-N-acetyl-galactosaminyl transferase 2"
                     /codon_start=1
                     /product="beta-1,4 N-acetylgalactosaminyltransferase 2
                     isoform b"
                     /protein_id="NP_001152859.1"
                     /db_xref="CCDS:CCDS54139.1"
                     /db_xref="GeneID:124872"
                     /db_xref="HGNC:HGNC:24136"
                     /db_xref="MIM:111730"
                     /translation="MTSGGSRFLWLLKILVIILVLGIVGFMFGSMFLQAVFSSPKPEL
                     PSPAPGVQKLKLLPEERLRNLFSYDGIWLFPKNQCKCEANKEQGGYNFQDAYGQSDLP
                     AVKARRQAEFEHFQRREGLPRPLPLLVQPNLPFGYPVHGVEVMPLHTVPIPGLQFEGP
                     DAPVYEVTLTASLGTLNTLADVPDSVVQGRGQKQLIISTSDRKLLKFILQHVTYTSTG
                     YQHQKVDIVSLESRSSVAKFPVTIRHPVIPKLYDPGPERKLRNLVTIATKTFLRPHKL
                     MIMLRSIREYYPDLTVIVADDSQKPLEIKDNHVEYYTMPFGKGWFAGRNLAISQVTTK
                     YVLWVDDDFLFNEETKIEVLVDVLEKTELDVVGGSVLGNVFQFKLLLEQSENGACLHK
                     RMGFFQPLDGFPSCVVTSGVVNFFLAHTERLQRVGFDPRLQRVAHSEFFIDGLGTLLV
                     GSCPEVIIGHQSRSPVVDSELAALEKTYNTYRSNTLTRVQFKLALHYFKNHLQCAA"
     exon            39..239
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            240..377
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            378..484
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            485..522
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            523..703
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..790
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            791..978
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1119
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1339
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..8657
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3893..3898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: ATTAAA"
     polyA_site      3911
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="major polyA site"
     regulatory      5027..5032
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: AATAAA"
     polyA_site      5058
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="major polyA site"
     regulatory      6424..6429
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: TATAAA"
     polyA_site      6453
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
     regulatory      7218..7223
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: AATATA"
     polyA_site      7237
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
     regulatory      8623..8628
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
                     /note="hexamer: AATAAA"
     polyA_site      8657
                     /gene="B4GALNT2"
                     /gene_synonym="B4GALT; GALGT2"
ORIGIN      
        1 agtctgaggg cggcgggatt cgggatgact tcgggcggct cgagatttct gtggctcctc
       61 aagatattgg tcataatcct ggtacttggc attgttggat ttatgttcgg aagcatgttc
      121 cttcaagcag tgttcagcag ccccaagcca gaactcccaa gtcctgcccc gggtgtccag
      181 aagctgaagc ttctgcctga ggaacgtctc aggaacctct tttcctacga tggaatctgg
      241 ctgttcccga aaaatcagtg caaatgtgaa gccaacaaag agcagggagg ttacaacttt
      301 caggatgcct atggccagag cgacctccca gcggtgaaag cgaggagaca ggctgaattt
      361 gaacactttc agaggagaga agggctgccc cgcccactgc ccctgctggt ccagcccaac
      421 ctcccctttg ggtacccagt ccacggagtg gaggtgatgc ccctgcacac ggttcccatc
      481 ccaggcctcc agtttgaagg acccgatgcc cccgtctatg aggtcaccct gacagcttct
      541 ctggggacac tgaacaccct tgctgatgtc ccagacagtg tggtgcaggg cagaggccag
      601 aagcagctga tcatttctac cagtgaccgg aagctgttga agttcattct tcagcacgtg
      661 acatacacca gcacggggta ccagcaccag aaggtagaca tagtgagtct ggagtccagg
      721 tcctcagtgg ccaagtttcc agtgaccatc cgccatcctg tcatacccaa gctatacgac
      781 cctggaccag agaggaagct cagaaacctg gttaccattg ctaccaagac tttcctccgc
      841 ccccacaagc tcatgatcat gctccggagt attcgagagt attacccaga cttgaccgta
      901 atagtggctg atgacagcca gaagcccctg gaaattaaag acaatcacgt ggagtattac
      961 actatgccct ttgggaaggg ttggtttgct ggtaggaacc tggccatatc tcaggtcacc
     1021 accaaatacg ttctctgggt ggacgatgat tttctcttca acgaggagac caagattgag
     1081 gtgctggtgg atgtcctgga gaaaacagaa ctggacgtgg taggcggcag tgtgctggga
     1141 aatgtgttcc agtttaagtt gttgctggaa cagagtgaga atggggcctg ccttcacaag
     1201 aggatgggat ttttccaacc cctggatggc ttccccagct gcgtggtgac cagtggcgtg
     1261 gtcaacttct tcctggccca cacggagcga ctccaaagag ttggctttga tccccgcctg
     1321 caacgagtgg ctcactcaga attcttcatt gatgggctag ggaccctact cgtggggtca
     1381 tgcccagaag tgattatagg tcaccagtct cggtctccag tggtggactc agaactggct
     1441 gccctagaga agacctacaa tacataccgg tccaacaccc tcacccgggt ccagttcaag
     1501 ctggccctcc actacttcaa gaaccatctc caatgtgccg cataaaggtg tgagggcata
     1561 ggagaaacac taggctggct ggttatggta tctatagcag gccaccaaaa actggactcc
     1621 tgataggtga acgttgtacc aaaccagctg gtgggtaggg aaaagggaaa tggctcagtt
     1681 actggaagta ccaatcaaag gtgaagggtc actggaaatg aaccagtcac tgaccagggc
     1741 aatggagact gtattaatag caatgatgat ttgtacaatg ccctgccttt tttgaagcat
     1801 ttgcatgggc agtatctcac atcatctcat ctgatatcac acaaagatga cattggttgg
     1861 tgtcctcagt caaaagaaca gctgcctggt ccttgatagt tgtttggtgg aaattctgtc
     1921 acagtcagaa gcatcggatc cagcatcact gtcatcccta tatttttatt aggttctatc
     1981 ttgtagactc aaaaaatgtg aacattccag ggtccttgcc ttgaggaggt ttgcagcctg
     2041 gttcagttca gtccaatcac agaattaggc agccggggtc agaggatcag agaagggaag
     2101 gggtcaccag gtcccaggtg gtggaggaag actccgtgga gtgaacttgt taggagcctg
     2161 aaagcagaca ttcaggcatc agagagggta ggagggccct ggggccatcc cactgtccac
     2221 cagaacaaag gctgccctgg catcagactc actccagacc tgttccagaa tgccagggat
     2281 tgaactcaga gcccatgcca aggcatgatg gtgaacagac agtgtgagtg tggtgacaag
     2341 ccactaaact ccagggacct gaggccagtt gctggtcact caaaactcat gtcatttaca
     2401 gagtggttcc atcactctgt tgttggataa gcctctttct gttgcctggg ggttggcaga
     2461 ttgccgagac cagcttggtc atggagaccc taacccagtg gtgctagagg aattaaagac
     2521 acacacatag aaatatagag tgtggagtgg gaaatcaggg gactcacaga cttcagagct
     2581 gagagccctg aacggagttt gacccacata tttattgaca gcaagccagt gataagcatt
     2641 gtttctatag attatagata aactaaaagt attccttaca ggaaacaaag ggatgggccg
     2701 aaacaaaggg atgggctctg gctagttatc tgcagcagga acatgtcctt aaagcacaga
     2761 tagctcatac tattatttgt ggcttaggaa tgctttaagc ggttttccac cctgggtggg
     2821 ccaggtgttc cttgccctta ttctgataaa cccacaacct tcagtttggg catcatggcc
     2881 atcatgaaca tgtcacagta ctgcagagat tttgtttatg gccagttttg gggccagttt
     2941 atggccagat ttgggggcct gttcccaaca cgttcccctt tttgttttcc aaagtgataa
     3001 aagcaaaggc agctttgtca tggtgagcta attctcacag gagtcaggat ccgcacctgc
     3061 agactataca aagacaaaca acacagatta aaagcacaat catcattgaa atcacagagc
     3121 ttccaagtgt ctttatccac tttaatgggt taatatctgc taatctgtct gcagctcctt
     3181 caagcactcc agttcctggc attaaggtca agtgtgcctg ggatgcttta aatatttgtt
     3241 cttttaattt tgcaatatgc aaagacaagt ttgtagagtg tccttctaga tgctttttta
     3301 ttctttccca aattttgatc ttattaacag acattaatag tttccacaaa tccttatgtt
     3361 tagcttctac agtggaccat atcatttgag gttgaggtgc cactataccg ccacgtttcc
     3421 agataatggg aactcttgct gtatttctta ccatttctac catctgactg ttttgtttag
     3481 accagctgaa catagtgtgg ctgtggcaca cagactgaga ggtgtaattt aagctaaaca
     3541 tccccttagg ggaccaatca ataatgattc cataggaatc gttgtgcagc acctctacct
     3601 gttctgcaat gcaatcttcc caaacaagta cattcattat ttctggccag gtctaattct
     3661 atttacaaat aggttttgag ctgcctttgc tttgcctcaa ttataggagc agatttatta
     3721 tggtaaatat taagagcaga aagcatgtgt aactgtgtca cacagtgatt acatccaggc
     3781 attattgcca gccaagattg ataaatatgc ccaataagta taactgttct ctgtgttgtt
     3841 ccttgttgaa ggaatactca tggcaatggt gatcaccact atcatatcta tcattaaatt
     3901 actcattgtg actggttgtc ccactttcct caggttttct tccaccatct gtgacagctt
     3961 cttgatctgt ccccaggtag gtggctgtgt tcgacagctg ttgctcatga tagttggggt
     4021 cctcctcagc atcagtcttg acatggctgc aaccagggga tcctcgggat cctcctggaa
     4081 tctcttcctc agcatctggc tcgtggcaag gtttcaggtg tcttgatggc acccaaattg
     4141 gctattgatt cggtcctgga gaaacacaag cataacctct accccaagtt attattttac
     4201 ctatttccca actttttgtt attgtatctc tccaccaaac cagttgttct gcttctgtct
     4261 ttgcagctgg tttctgtaga tgctgttcag ctgctggcct ttaagcaggc tcaaaaaatt
     4321 taaagtcaat aatgctagaa tcaattgcat atggggtgtc ccgtagtccc tgcttctccc
     4381 cttttgcttt tgcaactgct gtttcaggga gagattcatt ctttctacta tggcttgtcc
     4441 ttgagaatta tatgggatgc cagtaatgtg tttaatattc catatagaga aaaatgtagc
     4501 tagagcttgg ctagtacagc ctggggcatt atctgtttta atagaagctg gaatgcccat
     4561 caccgcaaaa cactgcaaaa ggtgacgttt aacacaggca gaagactctc ctgattggca
     4621 tgtagcccag acaaagtgag aaaaggtgtc cacacataca tgtacataag ctagtctccc
     4681 aaacgaggga acatgtgtga catccatttg ccaaagagaa ttaggttcca atccttgagg
     4741 attaactcct cctgtaaaag atgaggaatg caccatttgg caagttgggc atcgctggat
     4801 aatagcttta gcttctttcc aggtaatgcc gtatctgtgt ttgagaccag aggcattaac
     4861 ataggttaaa ttgtgaaagt gtctagcgtt agatattgca ttagcaacta ggtgatcagc
     4921 catttgattc ccttcagtta aaggtcctgg aagaggtgta tgagccctaa tgtgagtgat
     4981 gtaaaaagga tgcattctac tcctgactgc tgtttgcaat tgggtaaata aagccatcag
     5041 ttgctcatct gtgtggaatc gtagttgagc attttcaatt aattgtgtgg aatgaatcat
     5101 gtatgaagaa tcagaaatca cattgatagg cctattaaaa gcagtcaata cctcaattac
     5161 agctataagc tctgcttttt gaactgaagt atagggcatc tgaagccctt acttttcaag
     5221 ccagagttag aagctttacc attactagac ccatctgtga agacattttc agcaccttca
     5281 agtggtttga atttagttat tttagggaga atccaattag ttaatttcaa aaattgaaac
     5341 aattttgttt tgggaaaatg attatcaaga atacccacaa agtcagctaa atgggtttgc
     5401 caggtaagac tatttataaa agcttgctct atttgtgcct tcatgagaag gacaataatt
     5461 tttccaggac catatccatg taatttaaca atctgagttc tcccatttcc tatcatagta
     5521 gcgatttgat ccaaataagg agttagagtc cgtgaattag tatgtggaag aaaaagccgc
     5581 tctacaagat cttgctcttg aacaataaca ccagtaggtg aatgccgagt tgaaaaaatt
     5641 agcaaatcta gagtcttttc tggatctatt atatttattt gagctttatg gacttgcttt
     5701 ttgatgagct gcagctctgc atcagcttct tttgttaatt gccgagggct atgagactag
     5761 catctcctct aaggttagaa aatggcatag gtaggaatgc ctagagtagg tcatatccaa
     5821 ttaatgtccc ctggtaattt ttgaaagtca tttaatgttt tcagttgatc cctacatatg
     5881 gttactttct gtggcacaat gatagtgtca tttactgaag tctccaagta ggagtaagga
     5941 gtagcagtct gaattttgtc aggagctata attaaaccag catgagaaat caaattttgc
     6001 aagtgatcat aacattggag taatatttct cgagtggggg gcagcacaaa gtatatcgtc
     6061 catataatga ataatgtaac actgtgaaaa ttttttatga gtaggttcaa ttgtttgccc
     6121 tacataagtc tggcaaattg ttggactgtt taacatgcct tgtggcaaca gtttccagtg
     6181 aaaatgctta gcaggctgca ggttgtttac tgcaggaatt gtaaatgcaa accattcaca
     6241 gtcctgctca gctaagggga tagtaaagaa acagtctttt aaatctatga ctattaaagg
     6301 ccaattcttt ggaatcatag caggagaagg cagtcctggc tgcaatgtcc ccataggttg
     6361 tataactgaa ttaatggctc ttaagtcagt taacagtctc catgtacctg attttttctt
     6421 aattataaaa actggagagt tccaagggga aaatgttgga gctatgtgtc ctttttctaa
     6481 ttgttcagta actaagtcct ctgagcctcc agtttctctt tacttagcag tcattgttct
     6541 atccaaattg gctcatctgt taaccatttt aaaggtatag gttctggaag cttaacaatg
     6601 gctgccatca aaaatgatat cctaaacctt ggcaggaact ttgtctttcc gcttgaagtg
     6661 gttccttcaa atcttgcaaa ttttttccta gtcccatacc agggacatac cccatttcat
     6721 gcaccatacg ttgactttga gggctataca attgctctag aattagaact tgtgctatcc
     6781 attgttgtaa caaatctctt tcccataaat gtataggtac agaagttata attggttgaa
     6841 tagtcccatg ttgtccatcg ggcccctcac aatgcaaaat ataattactt tgatatactt
     6901 caggggcttt accaactcca actatgttaa attgagtggg ttgaatttgc cacgtggaca
     6961 gccagtgctg tagagaaatg attgaaatgt ctgctcctgt atctaccaaa cctttaaatt
     7021 tctttccctg aatagttatt tcacaggtag gatgtttatc agtaatttga ttcacccaat
     7081 aagctgcttt gtcttgttta tttgtgcttc caaattctcc tattcgttta atttcacttt
     7141 tccccatttc cacatacggc acaatcagaa gttgtgctat acgctttcct ggctctgctt
     7201 tccaaggaaa agaagtaaat ataacaattt gaatttcccc attgtaatct gaatcaatga
     7261 ctcctgtatg tacttgcact ccttttaaat ttaaactaga tctacctaga agtaatccta
     7321 tcatcccccc ggcaagcatc cacagacccc tattggaact ttttgtgggg attccccaag
     7381 cataaggctc acagcttttg tgcagtataa atctactgtg gcactactgg ctgtcgtggg
     7441 ggacagacat tgtacagggg tgagggagtg agctataata tttccctgtt gatctggggg
     7501 gtatatccta acagggaact gccaagcctc tatatcaccc tctcgtctag ctggctcaat
     7561 tcctgcctga atagaactga gagcagtcac tcgaggcact gctagaacag tcactggggc
     7621 aactactttt cacccagtgt cctctggaaa agaaagatct gtagggtcag gtcactcttt
     7681 ttcttcaaaa taatgagggg gtgaagaaga gtagggacaa acctcttcct catttgccgc
     7741 tttagcttca gccagcaaac aaacctgctg tgtcatctct tctgttactt tgttatactt
     7801 tccttccttc tcatcattag tgtgaaaagg ttctaaggtg gaacgcacca gagcccacac
     7861 ttgtcccatt gttaccctga tgtttccgag ctccccttct tactcaccat ggggattgct
     7921 taagggtact cgggtgtcct ccagcttagt tccacattct ccaaccatca ctccagcgac
     7981 ccttcaactg gggttcaagc cccacgttgg gcgccacttg ccgacaccag ctcggtcttg
     8041 gagaccctaa cccagcggcg ctagaggaat taaagacaca cacacagaaa tatagagtgt
     8101 ggagtgggaa atcaggggac tcacagcctt cagagctgaa agccccgaac agagtttgac
     8161 tcacatgttt attgacagca agccagtgat aagcattgtt tctaaagatt atagattaac
     8221 taaaagtatt ccttatggga aacaaaggga tggactgaaa caaagggatg ggctctggct
     8281 agttatctgc cacgtcctta aggcacagct cgctcatgct attgtttgtg gcttaggaac
     8341 accttaagag gttttccccc ctgggtggga caggtgttcc ttgccctcat tccagtaaat
     8401 ccacaacctt cagtgtgtgc atcatggcca tcatgaacat gtcacagtgc tgcagagatt
     8461 ttatttatgg ccagtttatg gccagatttg ggggcctgtt cccaacagca gattctatga
     8521 ccacttcagc cctgccttta cagtttctgt ttacctgtaa actttttctt cctgtggcct
     8581 agggtagttc aggatcacta aaaatgtgtt ctgtgatata ttaataaaaa ttcctgaaaa
     8641 atggacttac agcaaaa
//
