LOCUS       NM_032047               4108 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens UDP-GlcNAc:betaGal
            beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5), mRNA.
ACCESSION   NM_032047
VERSION     NM_032047.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Jeong,H.Y., Park,S.Y., Kim,H.J., Moon,S., Lee,S., Lee,S.H. and
            Kim,S.H.
  TITLE     B3GNT5 is a novel marker correlated with stem-like phenotype and
            poor clinical outcome in human gliomas
  JOURNAL   CNS Neurosci Ther 26 (11), 1147-1154 (2020)
   PUBMED   32677340
  REMARK    GeneRIF: B3GNT5 is a novel marker correlated with stem-like
            phenotype and poor clinical outcome in human gliomas.
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Zouboulis,C.C., Nogueira da Costa,A., Fimmel,S. and Zouboulis,K.C.
  TITLE     Apocrine glands are bystanders in hidradenitis suppurativa and
            their involvement is gender specific
  JOURNAL   J Eur Acad Dermatol Venereol 34 (7), 1555-1563 (2020)
   PUBMED   32031713
REFERENCE   3  (bases 1 to 4108)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 4108)
  AUTHORS   Wang,Z., Wen,L., Ma,X., Chen,Z., Yu,Y., Zhu,J., Wang,Y., Liu,Z.,
            Liu,H., Wu,D., Zhou,D. and Li,Y.
  TITLE     High expression of lactotriaosylceramide, a
            differentiation-associated glycosphingolipid, in the bone marrow of
            acute myeloid leukemia patients
  JOURNAL   Glycobiology 22 (7), 930-938 (2012)
   PUBMED   22411838
  REMARK    GeneRIF: bone marrow samples of acute myeloid leukemia patients had
            16-fold higher expression of B3GNT5 than those of healthy donors
REFERENCE   5  (bases 1 to 4108)
  AUTHORS   Marcos,N.T., Magalhaes,A., Ferreira,B., Oliveira,M.J.,
            Carvalho,A.S., Mendes,N., Gilmartin,T., Head,S.R., Figueiredo,C.,
            David,L., Santos-Silva,F. and Reis,C.A.
  TITLE     Helicobacter pylori induces beta3GnT5 in human gastric cell lines,
            modulating expression of the SabA ligand sialyl-Lewis x
  JOURNAL   J Clin Invest 118 (6), 2325-2336 (2008)
   PUBMED   18483624
  REMARK    GeneRIF: Helicobacter pylori induces beta3GnT5 in human gastric
            cell lines, modulating expression of the SabA ligand sialyl-Lewis x
REFERENCE   6  (bases 1 to 4108)
  AUTHORS   Togayachi,A., Akashima,T., Ookubo,R., Kudo,T., Nishihara,S.,
            Iwasaki,H., Natsume,A., Mio,H., Inokuchi,J., Irimura,T., Sasaki,K.
            and Narimatsu,H.
  TITLE     Molecular cloning and characterization of
            UDP-GlcNAc:lactosylceramide beta
            1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential
            enzyme for the expression of HNK-1 and Lewis X epitopes on
            glycolipids
  JOURNAL   J Biol Chem 276 (25), 22032-22040 (2001)
   PUBMED   11283017
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP270576.1, AK074424.1 and
            AK074235.1.
            
            On Nov 22, 2018 this sequence version replaced NM_032047.4.
            
            Summary: This gene encodes a member of the
            beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a
            type II membrane protein. It exhibits strong activity to transfer
            GlcNAc to glycolipid substrates and is identified as the most
            likely candidate for lactotriaosylceramide synthase. This enzyme is
            essential for the expression of Lewis X epitopes on glycolipids.
            [provided by RefSeq, Jul 2008].
            
            ##Evidence-Data-START##
            Transcript exon combination :: DA738808.1, SRR1660805.122771.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000326505.4/ ENSP00000316173.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 BP270576.1         10-13
            5-1764              AK074424.1         1-1760
            1765-4108           AK074235.1         732-3075
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q27.1"
     gene            1..4108
                     /gene="B3GNT5"
                     /gene_synonym="B3GN-T5; beta3Gn-T5"
                     /note="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 5"
                     /db_xref="GeneID:84002"
                     /db_xref="HGNC:HGNC:15684"
                     /db_xref="MIM:615333"
     exon            1..220
                     /gene="B3GNT5"
                     /gene_synonym="B3GN-T5; beta3Gn-T5"
                     /inference="alignment:Splign:2.1.0"
     exon            221..4108
                     /gene="B3GNT5"
                     /gene_synonym="B3GN-T5; beta3Gn-T5"
                     /inference="alignment:Splign:2.1.0"
     CDS             522..1658
                     /gene="B3GNT5"
                     /gene_synonym="B3GN-T5; beta3Gn-T5"
                     /EC_number="2.4.1.206"
                     /note="beta 1,3 N-acetyglucosaminyltransferase Lc3
                     synthase; beta-1,3-N-acetylglucosaminyltransferase bGnT-5;
                     BGnT-5; lc3 synthase; beta-1,3-Gn-T5; lc(3)Cer synthase;
                     lactotriaosylceramide synthase;
                     beta-1,3-N-acetylglucosaminyltransferase 5;
                     UDP-GlcNAc:beta-Gal
                     beta-1,3-N-acetylglucosaminyltransferase 5"
                     /codon_start=1
                     /product="lactosylceramide
                     1,3-N-acetyl-beta-D-glucosaminyltransferase"
                     /protein_id="NP_114436.1"
                     /db_xref="CCDS:CCDS3244.1"
                     /db_xref="GeneID:84002"
                     /db_xref="HGNC:HGNC:15684"
                     /db_xref="MIM:615333"
                     /translation="MRMLVSGRRVKKWQLIIQLFATCFLASLMFFWEPIDNHIVSHMK
                     SYSYRYLINSYDFVNDTLSLKHTSAGPRYQYLINHKEKCQAQDVLLLLFVKTAPENYD
                     RRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGEELQRKLAWEDQRYNDIIQQD
                     FVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSLEQIGVQDF
                     WIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLN
                     SSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWK
                     NATDPKVKTISKGFFGQIYCRLMKIILLCKISYVDTYPCRAAFI"
     regulatory      4089..4094
                     /regulatory_class="polyA_signal_sequence"
                     /gene="B3GNT5"
                     /gene_synonym="B3GN-T5; beta3Gn-T5"
                     /note="hexamer: AATAAA"
     polyA_site      4108
                     /gene="B3GNT5"
                     /gene_synonym="B3GN-T5; beta3Gn-T5"
                     /note="major polyA site"
ORIGIN      
        1 gcatacagcc cgcatcccgc cggggaagcg agcccagtcc agcgctgccc gtccagtcct
       61 cgcccaagat ttaaagcccg caagttttgt tcttgagacc agcgacttta gctccgatgc
      121 gggaaggaaa gccgacctcc gatttggaca tttaaagagc tgggcttgaa cttcgtgagt
      181 ttcgctctaa actgcccttg aaatgaagct ggacttggag gtggcatgga atattcacat
      241 gggagagccg catgaggccg cccaccacgc ttcctgaagg atgcccgtgt ggaagaattt
      301 tgacgtgcca gtgtcctcgt tctacagggt gttccattct tccgcaatct cagaaaaatg
      361 ggactaaaag aaactatttt gtaaaataag aagacttcca tttttaatga ccaacatgta
      421 ttaagatgga cacctactct acgaaacacg aagttctatg gtctcgaaga agcccgtgcc
      481 tgtttaaaac tgatcctaac taaaaacaga cttgagtgga tatgagaatg ttggttagtg
      541 gcagaagagt caaaaaatgg cagttaatta ttcagttatt tgctacttgt tttttagcga
      601 gcctcatgtt tttttgggaa ccaatcgata atcacattgt gagccatatg aagtcatatt
      661 cttacagata cctcataaat agctatgact ttgtgaatga taccctgtct cttaagcaca
      721 cctcagcggg gcctcgctac caatacttga ttaaccacaa ggaaaagtgt caagctcaag
      781 acgtcctcct tttactgttt gtaaaaactg ctcctgaaaa ctatgatcga cgttccggaa
      841 ttagaaggac gtggggcaat gaaaattatg ttcggtctca gctgaatgcc aacatcaaaa
      901 ctctgtttgc cttaggaact cctaatccac tggagggaga agaactacaa agaaaactgg
      961 cttgggaaga tcaaaggtac aatgatataa ttcagcaaga ctttgttgat tctttctaca
     1021 atcttactct gaaattactt atgcagttca gttgggcaaa tacctattgt ccacatgcca
     1081 aatttcttat gactgctgat gatgacatat ttattcacat gccaaatctg attgagtacc
     1141 ttcaaagttt agaacaaatt ggtgttcaag acttttggat tggtcgtgtt catcgtggtg
     1201 cccctcccat tagagataaa agcagcaaat actacgtgtc ctatgaaatg taccagtggc
     1261 cagcttaccc tgactacaca gccggagctg cctatgtaat ctccggtgat gtagctgcca
     1321 aagtctatga ggcatcacag acactaaatt caagtcttta catagacgat gtgttcatgg
     1381 gcctctgtgc caataaaata gggatagtac cgcaggacca tgtgtttttt tctggagagg
     1441 gtaaaactcc ttatcatccc tgcatctatg aaaaaatgat gacatctcat ggacacttag
     1501 aagatctcca ggacctttgg aagaatgcta cagatcctaa agtaaaaacc atttccaaag
     1561 gtttttttgg tcaaatatac tgcagattaa tgaagataat tctcctttgt aaaattagct
     1621 atgtggacac atacccttgt agggctgcgt ttatctaata gtacttgaat gttgtatgtt
     1681 ttcactgtca ctgagtcaaa cctggatgaa aaaaaccttt aaatgttcgt ctatacccta
     1741 agtaaaatga ggacgaaaga caaatatttt gaaagcctag tccatcagaa tgtttctttg
     1801 attctagaag ctgtttaata tcacttatct acttcattgc ctaagttcat ttcaaagaat
     1861 ttgtatttag aaaaggttta tattattagt gaaaacaaaa ctaaagggaa gttcaagttc
     1921 tcatgtaatg ccacatatat acttgaggtg tagagatgtt attaagaagt tttgatgtta
     1981 gaataattgc ttttggaaaa taccaaatga acgtacagta caacatttca aggaaatgaa
     2041 tatattgtta gaccaggtaa gcaagtttat ttttgttaaa gagcacttgg tggaggtagt
     2101 aggggcaggg aaaggtcagc ataggagaga aagttcatga atctggtaaa acagtctctt
     2161 gttcttaaga ggagatgtag aaaaatgtgt acaatgttat tataaacaga caaatcacgt
     2221 cttaccacat ccatgtagct actggtgtta gagtcattaa aatacctttt tttgcatctt
     2281 ttttcaaagt ttaatgtgaa cttttagaaa agtgattaat gttgccctaa tactttatat
     2341 gtttttaatg gatttttttt taagtattag aaaatgacac ataacacggg cagctggttg
     2401 ctcatagggt ccttctctag ggagaaacca ttgttaattc aaataagctg attttaatga
     2461 cgttttcaac tggtttttaa atattcaata ttggtctgtg tttaagtttg ttatttgaat
     2521 gtaatttaca tagaggaata taataatgga gagacttcaa atggaaagac agaacattac
     2581 aagcctaatg tctccataat tttataaaat gaaatcttag tgtctaaatc cttgtactga
     2641 ttactaaaat taacccactc ctccccaaca aggtcttata aaccacagca ctttgttcca
     2701 agttcagagt tttaaattga gagcattaaa catcaaagtt ataatatcta aaacaattta
     2761 tttttcatca ataactgtca gaggtgatct ttattttcta aatatttcaa acttgaaaac
     2821 agagtaaaaa agtgatagaa aagttgccag tttggggtta aagcattttt aaagctgcat
     2881 gttccttgta atcaaagaga tgtgtctgag atctaataga gtaagttaca tttattttac
     2941 aaagcaggat aaaaatgtgg ctataataca cactacctcc cttcactaca gaaagaacta
     3001 ggtggtgtct actgctaggg agattatatg aaggccaaaa taatgacttc agcaagagtg
     3061 actgaactca ctctaaggcc tttgactgca gaggcacctg ttagggaaaa tcagatgtct
     3121 catataataa ggtgatgtcg gaaacacgca aaacaaaacg aaaaaagatt tctcagtata
     3181 cacaactgaa tgatgatact tacaattttt agcaggtagc tttttaatgt ttacagaaat
     3241 tttaattttt ttctattttg aaatttgagg cttgtttaca ttgcttagat aatttagaat
     3301 ttttaactaa tgtcaaaact acagtgtcaa acattctagg ttgtagttac tttcagagta
     3361 gatacagggt tttagatcat tacagtttaa gttttctgac caattaaaaa aacatagaga
     3421 acaaaagcat atttgaccaa gcaacaagct tataattaat ttttattagt tgattgatta
     3481 atgatgtatt gccttttgcc catatatacc ctgtgtatct atacttggaa gtgtttaagg
     3541 ttgccattgg ttgaaaacat aagtgtctct ggccatcaaa gtgatcttgt ttacagcagt
     3601 gcttttgtga aacaattatt tatttgctga aagagctctt ctgaactgtg tccttttaat
     3661 ttttgcttag aatagaatgg aacaagttta aatttcaagg aaatatgaag gcacttcctt
     3721 tttttctaag aaggaagttg ctagatgatt ccttcatcac acttacttaa agtactgaga
     3781 agagtatctg taaataaaag ggttccaacc ttttaaaaaa gaaggaaaaa actttttggt
     3841 gctccagtgt agggctatct ttttaaaaaa tgtcaacaaa gggaaaataa actatcagct
     3901 tggatggtca cttgaataga agatggttat acacagtgtt attgttaaaa tttttttacc
     3961 ttttggttgg tttgcatctt ttttccatat tgttaatttt ataccaaaat gttaaatatt
     4021 tgtattactt gaattttgct cttgtatggc aaaataatta gtgagtttaa aaaaaatcta
     4081 tagtttccaa taaacaactg aaaaatta
//
