LOCUS       NM_001700                907 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens azurocidin 1 (AZU1), mRNA.
ACCESSION   NM_001700
VERSION     NM_001700.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Johannesson,E., Erixon,C., Sterner,N., Thelaus,L., Dardashti,A.,
            Nilsson,J., Ragnarsson,S., Linder,A. and Zindovic,I.
  TITLE     Utility of heparin-binding protein following cardiothoracic surgery
            using cardiopulmonary bypass
  JOURNAL   Sci Rep 13 (1), 21566 (2023)
   PUBMED   38057352
  REMARK    GeneRIF: Utility of heparin-binding protein following
            cardiothoracic surgery using cardiopulmonary bypass.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  AUTHORS   Xu,Z., Li,Q. and Shen,X.
  TITLE     AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes
            affecting the severity of acute mountain sickness
  JOURNAL   BMC Med Genomics 16 (1), 28 (2023)
   PUBMED   36803152
  REMARK    GeneRIF: AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate
            genes affecting the severity of acute mountain sickness.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 907)
  AUTHORS   Chen,J.Q., Duan,Y.F. and Wang,J.M.
  TITLE     Increased CAP37 Expression in Stable Chronic Obstructive Pulmonary
            Disease
  JOURNAL   Curr Med Sci 42 (5), 949-957 (2022)
   PUBMED   36245028
  REMARK    GeneRIF: Increased CAP37 Expression in Stable Chronic Obstructive
            Pulmonary Disease.
REFERENCE   4  (bases 1 to 907)
  AUTHORS   McCabe,D., Cukierman,T. and Gabay,J.E.
  TITLE     Basic residues in azurocidin/HBP contribute to both heparin binding
            and antimicrobial activity
  JOURNAL   J Biol Chem 277 (30), 27477-27488 (2002)
   PUBMED   11994286
  REMARK    GeneRIF: Azurocidin 1 displays antimicrobial activity against E.
            coli, S. faecalis, and C. albicans.
REFERENCE   5  (bases 1 to 907)
  AUTHORS   Lindmark,A., Garwicz,D., Rasmussen,P.B., Flodgaard,H. and
            Gullberg,U.
  TITLE     Characterization of the biosynthesis, processing, and sorting of
            human HBP/CAP37/azurocidin
  JOURNAL   J Leukoc Biol 66 (4), 634-643 (1999)
   PUBMED   10534120
REFERENCE   6  (bases 1 to 907)
  AUTHORS   Karlsen,S., Iversen,L.F., Larsen,I.K., Flodgaard,H.J. and
            Kastrup,J.S.
  TITLE     Atomic resolution structure of human HBP/CAP37/azurocidin
  JOURNAL   Acta Crystallogr D Biol Crystallogr 54 (Pt 4), 598-609 (1998)
   PUBMED   9761855
REFERENCE   7  (bases 1 to 907)
  AUTHORS   Zimmer,M., Medcalf,R.L., Fink,T.M., Mattmann,C., Lichter,P. and
            Jenne,D.E.
  TITLE     Three human elastase-like genes coordinately expressed in the
            myelomonocyte lineage are organized as a single genetic locus on
            19pter
  JOURNAL   Proc Natl Acad Sci U S A 89 (17), 8215-8219 (1992)
   PUBMED   1518849
REFERENCE   8  (bases 1 to 907)
  AUTHORS   Morgan,J.G., Sukiennicki,T., Pereira,H.A., Spitznagel,J.K.,
            Guerra,M.E. and Larrick,J.W.
  TITLE     Cloning of the cDNA for the serine protease homolog
            CAP37/azurocidin, a microbicidal and chemotactic protein from human
            granulocytes
  JOURNAL   J Immunol 147 (9), 3210-3214 (1991)
   PUBMED   1919011
REFERENCE   9  (bases 1 to 907)
  AUTHORS   Almeida,R.P., Melchior,M., Campanelli,D., Nathan,C. and Gabay,J.E.
  TITLE     Complementary DNA sequence of human neutrophil azurocidin, an
            antibiotic with extensive homology to serine proteases
  JOURNAL   Biochem Biophys Res Commun 177 (2), 688-695 (1991)
   PUBMED   2049091
REFERENCE   10 (bases 1 to 907)
  AUTHORS   Green,B.G., Weston,H., Ashe,B.M., Doherty,J., Finke,P., Hagmann,W.,
            Lark,M., Mao,J., Maycock,A., Moore,V. et al.
  TITLE     PMN elastases: a comparison of the specificity of human isozymes
            and the enzyme from other species toward substrates and inhibitors
  JOURNAL   Arch Biochem Biophys 286 (1), 284-292 (1991)
   PUBMED   1897955
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC093931.1 and BU617927.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001700.4.
            
            Summary: Azurophil granules, specialized lysosomes of the
            neutrophil, contain at least 10 proteins implicated in the killing
            of microorganisms. This gene encodes a preproprotein that is
            proteolytically processed to generate a mature azurophil granule
            antibiotic protein, with monocyte chemotactic and antimicrobial
            activity. It is also an important multifunctional inflammatory
            mediator. This encoded protein is a member of the serine protease
            gene family but it is not a serine proteinase, because the active
            site serine and histidine residues are replaced. The genes encoding
            this protein, neutrophil elastase 2, and proteinase 3 are in a
            cluster located at chromosome 19pter. All 3 genes are expressed
            coordinately and their protein products are packaged together into
            azurophil granules during neutrophil differentiation. [provided by
            RefSeq, Nov 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X58794.1, ERR279847.7758.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145893, SAMEA2146236
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match                 :: ENST00000233997.4/
                                                  ENSP00000233997.1
            Protein has antimicrobial activity :: PMID: 11994286
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-794               BC093931.1         12-805
            795-907             BU617927.1         19-131              c
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..907
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /note="azurocidin 1"
                     /db_xref="GeneID:566"
                     /db_xref="HGNC:HGNC:913"
                     /db_xref="MIM:162815"
     exon            1..68
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..766
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /note="cationic antimicrobial protein 37; heparin-binding
                     protein; neutrophil azurocidin; cationic antimicrobial
                     protein CAP37"
                     /codon_start=1
                     /product="azurocidin preproprotein"
                     /protein_id="NP_001691.1"
                     /db_xref="CCDS:CCDS12044.1"
                     /db_xref="GeneID:566"
                     /db_xref="HGNC:HGNC:913"
                     /db_xref="MIM:162815"
                     /translation="MTRLTVLALLAGLLASSRAGSSPLLDIVGGRKARPRQFPFLASI
                     QNQGRHFCGGALIHARFVMTAASCFQSQNPGVSTVVLGAYDLRRRERQSRQTFSISSM
                     SENGYDPQQNLNDLMLLQLDREANLTSSVTILPLPLQNATVEAGTRCQVAGWGSQRSG
                     GRLSRFPRFVNVTVTPEDQCRPNNVCTGVLTRRGGICNGDGGTPLVCEGLAHGVASFS
                     LGPCGRGPDFFTRVALFRDWIDGVLNNPGPGPA"
     exon            69..225
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /inference="alignment:Splign:2.1.0"
     exon            226..370
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /inference="alignment:Splign:2.1.0"
     exon            371..604
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /inference="alignment:Splign:2.1.0"
     exon            605..907
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      882..887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /note="hexamer: AATAAA"
     polyA_site      907
                     /gene="AZU1"
                     /gene_synonym="AZAMP; AZU; CAP37; HBP; hHBP; HUMAZUR;
                     NAZC"
                     /note="major polyA site"
ORIGIN      
        1 ctgccccgcc atgacccggc tgacagtcct ggccctgctg gctggtctgc tggcgtcctc
       61 gagggccggc tccagccccc ttttggacat cgttggcggc cggaaggcga ggccccgcca
      121 gttcccgttc ctggcctcca ttcagaatca aggcaggcac ttctgcgggg gtgccctgat
      181 ccatgcccgc ttcgtgatga ccgcggccag ctgcttccaa agccagaacc ccggggttag
      241 caccgtggtg ctgggtgcct atgacctgag gcggcgggag aggcagtccc gccagacgtt
      301 ttccatcagc agcatgagcg agaatggcta cgacccccag cagaacctga acgacctgat
      361 gctgcttcag ctggaccgtg aggccaacct caccagcagc gtgacgatac tgccactgcc
      421 tctgcagaac gccacggtgg aagccggcac cagatgccag gtggccggct gggggagcca
      481 gcgcagtggg gggcgtctct cccgttttcc caggtttgtc aacgtgactg tgacccccga
      541 ggaccagtgt cgccccaaca acgtgtgcac cggtgtgctc acccgccgcg gtggcatctg
      601 caatggggac gggggcaccc ccctcgtctg cgagggcctg gcccacggcg tggcctcctt
      661 ttccctgggg ccctgtggcc gaggccctga cttcttcacc cgagtggcgc tcttccgaga
      721 ctggatcgat ggtgttctca acaacccggg accggggcca gcctaggggg gcctgtgacc
      781 tcccatggag cccagccccg ccctccacac ctccggcgct ccgcacccac ctcccacggc
      841 cccgcccctg cccccgctcc ggccagaggg gccctggctg taataaagaa gccgatctct
      901 cctctgc
//
