LOCUS       NM_022461               4403 bp    mRNA    linear   PRI 12-NOV-2024
DEFINITION  Homo sapiens 5-azacytidine induced 2 (AZI2), transcript variant 1,
            mRNA.
ACCESSION   NM_022461
VERSION     NM_022461.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Endo,R., Kinefuchi,H., Sawada,M., Kikuchi,R., Kojima,W., Matsuda,N.
            and Yamano,K.
  TITLE     TBK1 adaptor AZI2/NAP1 regulates NDP52-driven mitochondrial
            autophagy
  JOURNAL   J Biol Chem 300 (10), 107775 (2024)
   PUBMED   39276928
  REMARK    GeneRIF: TBK1 adaptor AZI2/NAP1 regulates NDP52-driven
            mitochondrial autophagy.
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Yeo,S.K., Haas,M., Manupati,K., Hao,M., Yang,F., Chen,S. and
            Guan,J.L.
  TITLE     AZI2 mediates TBK1 activation at unresolved selective autophagy
            cargo receptor complexes with implications for CD8 T-cell
            infiltration in breast cancer
  JOURNAL   Autophagy 20 (3), 525-540 (2024)
   PUBMED   37733921
  REMARK    GeneRIF: AZI2 mediates TBK1 activation at unresolved selective
            autophagy cargo receptor complexes with implications for CD8 T-cell
            infiltration in breast cancer.
REFERENCE   3  (bases 1 to 4403)
  AUTHORS   Paul,S., Sarraf,S.A., Nam,K.H., Zavar,L., DeFoor,N., Biswas,S.R.,
            Fritsch,L.E., Yaron,T.M., Johnson,J.L., Huntsman,E.M.,
            Cantley,L.C., Ordureau,A. and Pickrell,A.M.
  TITLE     NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate
            mitosis and cytokinesis
  JOURNAL   J Cell Biol 223 (2) (2024)
   PUBMED   38059900
  REMARK    GeneRIF: NAK-associated protein 1/NAP1 activates TBK1 to ensure
            accurate mitosis and cytokinesis.
REFERENCE   4  (bases 1 to 4403)
  AUTHORS   Buljan,M., Ciuffa,R., van Drogen,A., Vichalkovski,A., Mehnert,M.,
            Rosenberger,G., Lee,S., Varjosalo,M., Pernas,L.E., Spegg,V.,
            Snijder,B., Aebersold,R. and Gstaiger,M.
  TITLE     Kinase Interaction Network Expands Functional and Disease Roles of
            Human Kinases
  JOURNAL   Mol Cell 79 (3), 504-520 (2020)
   PUBMED   32707033
REFERENCE   5  (bases 1 to 4403)
  AUTHORS   Shang,J., Xia,T., Han,Q.Q., Zhao,X., Hu,M.M., Shu,H.B. and Guo,L.
  TITLE     Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a
            Positive Regulator of SeV-Induced Innate Immunity
  JOURNAL   Proteomics 18 (2) (2018)
   PUBMED   29251827
REFERENCE   6  (bases 1 to 4403)
  AUTHORS   Sasai,M., Shingai,M., Funami,K., Yoneyama,M., Fujita,T.,
            Matsumoto,M. and Seya,T.
  TITLE     NAK-associated protein 1 participates in both the TLR3 and the
            cytoplasmic pathways in type I IFN induction
  JOURNAL   J Immunol 177 (12), 8676-8683 (2006)
   PUBMED   17142768
  REMARK    GeneRIF: Both the cytoplasmic and TLR3-mediated dsRNA recognition
            pathways converge upon NAP1 for the activation of the IRF-3 and
            IFN-beta promoter.
REFERENCE   7  (bases 1 to 4403)
  AUTHORS   Sasai,M., Oshiumi,H., Matsumoto,M., Inoue,N., Fujita,F.,
            Nakanishi,M. and Seya,T.
  TITLE     Cutting Edge: NF-kappaB-activating kinase-associated protein 1
            participates in TLR3/Toll-IL-1 homology domain-containing adapter
            molecule-1-mediated IFN regulatory factor 3 activation
  JOURNAL   J Immunol 174 (1), 27-30 (2005)
   PUBMED   15611223
REFERENCE   8  (bases 1 to 4403)
  AUTHORS   Bouwmeester,T., Bauch,A., Ruffner,H., Angrand,P.O., Bergamini,G.,
            Croughton,K., Cruciat,C., Eberhard,D., Gagneur,J., Ghidelli,S.,
            Hopf,C., Huhse,B., Mangano,R., Michon,A.M., Schirle,M., Schlegl,J.,
            Schwab,M., Stein,M.A., Bauer,A., Casari,G., Drewes,G., Gavin,A.C.,
            Jackson,D.B., Joberty,G., Neubauer,G., Rick,J., Kuster,B. and
            Superti-Furga,G.
  TITLE     A physical and functional map of the human TNF-alpha/NF-kappa B
            signal transduction pathway
  JOURNAL   Nat Cell Biol 6 (2), 97-105 (2004)
   PUBMED   14743216
  REMARK    Erratum:[Nat Cell Biol. 2004 May;6(5):465]
REFERENCE   9  (bases 1 to 4403)
  AUTHORS   Fujita,F., Taniguchi,Y., Kato,T., Narita,Y., Furuya,A., Ogawa,T.,
            Sakurai,H., Joh,T., Itoh,M., Delhase,M., Karin,M. and Nakanishi,M.
  TITLE     Identification of NAP1, a regulatory subunit of IkappaB
            kinase-related kinases that potentiates NF-kappaB signaling
  JOURNAL   Mol Cell Biol 23 (21), 7780-7793 (2003)
   PUBMED   14560022
  REMARK    GeneRIF: These results define NAK-associated protein 1 (NAP1) as an
            activator of IkappaB kinase-related kinases and suggest that the
            NAK-NAP1 complex may protect cells from TNF-alpha-induced apoptosis
            by promoting NF-kappaB activation.
REFERENCE   10 (bases 1 to 4403)
  AUTHORS   Miyagawa,J., Muguruma,M., Aoto,H., Suetake,I., Nakamura,M. and
            Tajima,S.
  TITLE     Isolation of the novel cDNA of a gene of which expression is
            induced by a demethylating stimulus
  JOURNAL   Gene 240 (2), 289-295 (1999)
   PUBMED   10580148
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK290637.1, AK025592.1, BX648471.1 and AC098616.2.
            
            On Nov 23, 2018 this sequence version replaced NM_022461.4.
            
            Summary: AZI2, or NAP1, contributes to the activation of NFKB (see
            MIM 164011)-dependent gene expression by activating IKK-related
            kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003
            [PubMed 14560022]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.239794.1,
                                           SRR1660803.212983.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000479665.6/ ENSP00000419371.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-984               AK290637.1         23-1006
            985-2033            AK025592.1         1135-2183
            2034-2095           BX648471.1         2065-2126
            2096-2429           BX648471.1         2128-2461
            2430-2431           AC098616.2         52826-52827
            2432-3093           BX648471.1         2462-3123
            3094-3094           AC098616.2         53490-53490
            3095-3097           BX648471.1         3125-3127
            3098-4403           AC098616.2         53494-54799
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p24.1"
     gene            1..4403
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="5-azacytidine induced 2"
                     /db_xref="GeneID:64343"
                     /db_xref="HGNC:HGNC:24002"
                     /db_xref="MIM:609916"
     exon            1..224
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     exon            225..445
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     CDS             230..1408
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="isoform a is encoded by transcript variant 1;
                     NAK-associated protein 1; 5-azacytidine induced gene 2;
                     5-azacytidine-induced protein 2; NF-kappa-B-activating
                     kinase-associated protein 1"
                     /codon_start=1
                     /product="5-azacytidine-induced protein 2 isoform a"
                     /protein_id="NP_071906.1"
                     /db_xref="CCDS:CCDS2647.1"
                     /db_xref="GeneID:64343"
                     /db_xref="HGNC:HGNC:24002"
                     /db_xref="MIM:609916"
                     /translation="MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTA
                     YEDIKKRLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVCID
                     RDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEV
                     EKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDN
                     MQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKACAPVGCSEDLGRDSTKLHLM
                     NFTATYTRHPPLLPNGKALCHTTSSPLPGDVKVLSEKAILQSWTDNERSIPNDGTCFQ
                     EHSSYGRNSLEDNSWVFPSPPKSSETAFGETKTKTLPLPNLPPLHYLDQHNQNCLYKN
                     "
     exon            446..568
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     exon            569..668
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     exon            669..817
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     exon            818..876
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     exon            877..995
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     exon            996..4403
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1887..1892
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="hexamer: AATAAA"
     polyA_site      1913
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="major polyA site"
     regulatory      3071..3076
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="hexamer: AATAAA"
     polyA_site      3096
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="major polyA site"
     regulatory      3173..3178
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="hexamer: AATAGA"
     polyA_site      3192
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
     polyA_site      3729
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
     regulatory      4366..4371
                     /regulatory_class="polyA_signal_sequence"
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
                     /note="hexamer: AGTAAA"
     polyA_site      4403
                     /gene="AZI2"
                     /gene_synonym="AZ2; NAP1; TILP"
ORIGIN      
        1 ctccttccag aacgtcccgg gctcctgccg agtcagaaga aatgggactc cctccgcgac
       61 gtgcccggag cagctccctt cgctgtggaa gcggcggtgt cttcgaagaa accggaagcc
      121 cgtggtgacc cctggcgacc cggtttgttt tcggtccgtt tccaaacact aaggaatcga
      181 aactcggcgg ccttgggggc ggccctacgt agcctggctt ctggttgtca tggatgcact
      241 ggtagaagat gatatctgta ttctgaatca tgaaaaagcc cataagagag atacagtgac
      301 tccagtttca atatattcag gagatgaatc tgttgcttcc cattttgctc ttgtcactgc
      361 atatgaagac atcaaaaaac gacttaagga ttcagagaaa gagaactctt tgttaaagaa
      421 gagaataaga tttttggaag aaaagctaat agctcgattt gaagaagaaa caagttccgt
      481 gggacgagaa caagtaaata aggcctatca tgcatatcga gaggtttgca ttgatagaga
      541 taatttgaag agcaaactgg acaaaatgaa taaagacaac tctgaatctt tgaaagtatt
      601 gaatgagcag ctacaatcta aagaagtaga actcctccag ctgaggacag aggtggaaac
      661 tcagcaggtg atgaggaatt taaatccacc ttcatcaaac tgggaggtgg aaaagttgag
      721 ctgtgacctg aagatccatg gtttggaaca agagctggaa ctgatgagga aagaatgtag
      781 cgatctcaaa atagaactac agaaagccaa acaaacggat ccatatcagg aagacaatct
      841 gaagagcaga gatctccaaa aactaagcat ttcaagtgat aatatgcagc atgcatactg
      901 ggaactgaag agagaaatgt ctaatttaca tctggtgact caagtacaag ctgaactact
      961 aagaaaactg aaaacctcaa ctgcaatcaa gaaagcctgt gcccctgtag gatgcagtga
     1021 agaccttgga agagacagca caaaactgca cttgatgaat tttactgcaa catacacaag
     1081 acatccccct ctcttaccaa atggcaaagc tctttgtcat accacatctt cccctttacc
     1141 aggagatgta aaggttttat cagagaaagc aatcctccaa tcatggacag acaatgagag
     1201 atccattcct aatgatggta catgctttca ggaacacagt tcttatggca gaaattctct
     1261 ggaagacaat tcctgggtat ttccaagtcc tcctaaatca agtgagacag catttgggga
     1321 aactaaaact aaaactttgc ctttacccaa ccttccacca ctgcattact tggatcaaca
     1381 taatcagaac tgcctttata agaattaatt tggaagagat tcacgatttc accatgagga
     1441 cacttatctc tttcagtggt cctcccaaga aattatttaa caaactgaaa ggagattttg
     1501 attaaaattt tgcagaggtc ttcagtatct atatttgaac acactgtaca atagtacaaa
     1561 aaccaacata gttggttttc tagtatgaaa gagcaccctc tagctccata ttctaagaat
     1621 ctgaaatatg ctactatact aattaataag taaacttaag gtgtttaaaa aactctgcct
     1681 tctatattaa ttgtaaaatt ttgcctctca gaagaatgga attggagatt gtagacgtgg
     1741 ttttacaaaa tgtgaaatgt ctaaatatct gttcataaaa ataaaaggaa aacatgtttc
     1801 ttcaaattgc ataatggaac aaatggcaat gtgagtaggt tacatttctg ttgttataat
     1861 gcgtaaagat attgaaaata taatgaaata aaagcatctt aggttatacc atctttatat
     1921 gctattgcgt ttcaatattt aagatttaaa gtgatttttt ggtcacagtg ttttgttgat
     1981 aaaatttttt tagaattgaa gtttgaattc taagacttga aacaacctga tcactgaagc
     2041 caactttgtc ccagcacatt ccttaagtcc taattgggaa aaaaaaaaaa aaaaatgaaa
     2101 tagttgaaaa actctggggt gtaaacaaat gattgtaacc ctacacacta ttcaataagt
     2161 agtagaagga gcatcacaca gatttcagtc taatctgccc ttctgtgggc cataatataa
     2221 acataaatgt gtgtaatgat aaaaagtcat tttcttcaaa gagactacag ctagctgcac
     2281 attgtgtaga gcagcttcta aattgttaga ctttgtgttg aaatgtaata ttctatttat
     2341 tgagaaagtg atttaaaatt attattttta atcatagaaa tcagggtttg ggctgtattg
     2401 atattgtcga tcatgaaatg tccacactta ttctaagtgg ccaattattt ggaaataaag
     2461 aaggaaataa agatggcttc acatggaaat ttaagttctt tcagggtgga gatttacttg
     2521 gttcatacac cttttgcctg aattaaagta tttcatgtag gaggactttt atcctttttg
     2581 atagacagtt tcatatatct tgaactcaat atctcagatc tcttctactg tattactgaa
     2641 tagcatacat acatagacaa tgttcgccat tcactagata tttttttcta ttatcttaca
     2701 cttattcaag cttgtctgtg attaatggaa ttggtgtcag atgctggaat ttattctgac
     2761 caatgaacac agctgactca ggggagtaca atctcctgcc aagtaataga accaaaccca
     2821 atatgcataa aagaaataca atactccagg ctttagctga aggaagcaac tacctgtgta
     2881 ataacaaagc agcaaaaact atttctcatg tggctgcata ggctgtatat tatatctgat
     2941 ctctaatgta gcttactggt ttgccttttt taaaaccaaa attggaaatt ttcctttgta
     3001 aagaaaaaaa gtcttatgag ataattgctt gattaatgtt ttgaacaata ccaagaaatt
     3061 gtttaattaa aataaatatt tttgtttgaa attgaagtgg atgtgaaata ttgtccttgc
     3121 ctatagatct caaaatctag tactaattgt tggttgttaa tatgattcat acaatagaca
     3181 tttgttaaac cagcttttga gtggagaaaa ttattttcca tttaaacagt acagtgatga
     3241 tagctatcaa acaggtatac taaaaagtca gtgactgtga cagtttaaag tgttagacaa
     3301 atgatgtata aggttcttta gtaaggaaag attagccaaa aattttttac ccagctagaa
     3361 gcttttaaat ctacacttga tatctctgtt ctaggtaaaa ttcaaacaac caaaaaacag
     3421 tggcccatct gtagcttatg aatctattta aaattgaata ttttatgttg acttctttca
     3481 tactagctga aacttgctct aaagagcttc ctttggaatc tttagatttc tttttataag
     3541 gttcaattca gtacatccat aaatcgacag aatgaagaaa acataacaaa aagccaccta
     3601 ttccttcctc ttgctccctt attagctaaa gtaaaattta catctgtaag acttactgag
     3661 ttgctgccaa aatggaactg aattcttaaa agcaggaaca tgcctatgtg aaacattaga
     3721 aaaccaaaat aaatgggaaa attcagactt gaagagctga atcatgtgac aacaaagtac
     3781 tgatcttaat tcagaaaggc tacttggaga aggtgtatct taaggggtaa aaaacctgtg
     3841 ggttaccaca tattaacagg gaataatatc aacagaggca cccataagca agcaagacac
     3901 aactgagaca gatgtaccag gtgaaaaaaa tcttcagtat ggatacacat gggtattctg
     3961 aaggctacaa tgaatggttt agtctttgac ataaggagga tgaacatatt atttatactg
     4021 tgtaatctaa tctggcttct atggggaaag caacatgtac ataagtgaat tttctacatg
     4081 aagtgggcta cttctgattt taatcaaagc tataaaatag attgtatatt tcactagctt
     4141 cttaaagtaa ctcttccatt ttcatttatc tctaaaaaca gttaacttcc tgtgctctag
     4201 gaaatgcacc aataccaaag gtcaatgtgg aaatatgggc atgtttgccc ctatgctgtg
     4261 tggtctctag atttctatat ttgctttgct tttgtctgct ctagtaatct ccctcctttt
     4321 gatctgtggc ctgggaaaat gtggtttctt tgtatttcaa ataaaagtaa aatagttttt
     4381 tgagtggttc actcaagagt aaa
//
