LOCUS       NM_004993               6884 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens ataxin 3 (ATXN3), transcript variant reference, mRNA.
ACCESSION   NM_004993
VERSION     NM_004993.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Cheng,Y., Wang,S., Gao,Q. and Fang,D.
  TITLE     ATXN3 functions as a tumor suppressor through potentiating
            galectin-9-mediated apoptosis in human colon adenocarcinoma
  JOURNAL   J Biol Chem 300 (7), 107415 (2024)
   PUBMED   38815863
  REMARK    GeneRIF: ATXN3 functions as a tumor suppressor through potentiating
            galectin-9-mediated apoptosis in human colon adenocarcinoma.
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Hou,C., Liu,J., Liu,J., Yao,D., Liang,F., Qin,C. and Ma,Z.
  TITLE     5-methylcytosine-mediated upregulation of circular RNA 0102913
            augments malignant properties of colorectal cancer cells through a
            microRNA-571/Rac family small GTPase 2 axis
  JOURNAL   Gene 901, 148162 (2024)
   PUBMED   38224924
  REMARK    GeneRIF: 5-methylcytosine-mediated upregulation of circular RNA
            0102913 augments malignant properties of colorectal cancer cells
            through a microRNA-571/Rac family small GTPase 2 axis.
REFERENCE   3  (bases 1 to 6884)
  AUTHORS   Raposo,M., Hubener-Schmid,J., Tagett,R., Ferreira,A.F., Vieira
            Melo,A.R., Vasconcelos,J., Pires,P., Kay,T., Garcia-Moreno,H.,
            Giunti,P., Santana,M.M., Pereira de Almeida,L., Infante,J., van de
            Warrenburg,B.P., de Vries,J.J., Faber,J., Klockgether,T.,
            Casadei,N., Admard,J., Schols,L., Riess,O., Costa,M.D.C. and
            Lima,M.
  CONSRTM   European Spinocerebellar ataxia type 3/Machado-Joseph disease
            Initiative (ESMI) study group
  TITLE     Blood and cerebellar abundance of ATXN3 splice variants in
            spinocerebellar ataxia type 3/Machado-Joseph disease
  JOURNAL   Neurobiol Dis 193, 106456 (2024)
   PUBMED   38423193
  REMARK    GeneRIF: Blood and cerebellar abundance of ATXN3 splice variants in
            spinocerebellar ataxia type 3/Machado-Joseph disease.
REFERENCE   4  (bases 1 to 6884)
  AUTHORS   Wang,S., Iyer,R., Han,X., Wei,J., Li,N., Cheng,Y., Zhou,Y., Gao,Q.,
            Zhang,L., Yan,M., Sun,Z. and Fang,D.
  TITLE     CRISPR screening identifies the deubiquitylase ATXN3 as a
            PD-L1-positive regulator for tumor immune evasion
  JOURNAL   J Clin Invest 133 (23), e167728 (2023)
   PUBMED   38038129
  REMARK    GeneRIF: CRISPR screening identifies the deubiquitylase ATXN3 as a
            PD-L1-positive regulator for tumor immune evasion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6884)
  AUTHORS   Bettencourt,C., Santos,C., Montiel,R., Costa Mdo,C.,
            Cruz-Morales,P., Santos,L.R., Simoes,N., Kay,T., Vasconcelos,J.,
            Maciel,P. and Lima,M.
  TITLE     Increased transcript diversity: novel splicing variants of
            Machado-Joseph disease gene (ATXN3)
  JOURNAL   Neurogenetics 11 (2), 193-202 (2010)
   PUBMED   19714377
  REMARK    GeneRIF: Data demonstrate high variability in the ATXN3 gene
            transcripts, providing a basis for further investigation on the
            contribution of alternative splicing to the MJD pathogenic process,
            as well as to the larger group of the polyglutamine disorders.
REFERENCE   6  (bases 1 to 6884)
  AUTHORS   Schols,L., Vieira-Saecker,A.M., Schols,S., Przuntek,H., Epplen,J.T.
            and Riess,O.
  TITLE     Trinucleotide expansion within the MJD1 gene presents clinically as
            spinocerebellar ataxia and occurs most frequently in German SCA
            patients
  JOURNAL   Hum Mol Genet 4 (6), 1001-1005 (1995)
   PUBMED   7655453
REFERENCE   7  (bases 1 to 6884)
  AUTHORS   Stevanin,G., Cancel,G., Durr,A., Chneiweiss,H., Dubourg,O.,
            Weissenbach,J., Cann,H.M., Agid,Y. and Brice,A.
  TITLE     The gene for spinal cerebellar ataxia 3 (SCA3) is located in a
            region of approximately 3 cM on chromosome 14q24.3-q32.2
  JOURNAL   Am J Hum Genet 56 (1), 193-201 (1995)
   PUBMED   7825578
REFERENCE   8  (bases 1 to 6884)
  AUTHORS   Kawaguchi,Y., Okamoto,T., Taniwaki,M., Aizawa,M., Inoue,M.,
            Katayama,S., Kawakami,H., Nakamura,S., Nishimura,M., Akiguchi,I. et
            al.
  TITLE     CAG expansions in a novel gene for Machado-Joseph disease at
            chromosome 14q32.1
  JOURNAL   Nat Genet 8 (3), 221-228 (1994)
   PUBMED   7874163
REFERENCE   9  (bases 1 to 6884)
  AUTHORS   Paulson,H. and Shakkottai,V.
  TITLE     Spinocerebellar Ataxia Type 3
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301375
REFERENCE   10 (bases 1 to 6884)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA827537.1, AB050194.1 and
            AL049872.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_004993.5.
            
            Summary: Machado-Joseph disease, also known as spinocerebellar
            ataxia-3, is an autosomal dominant neurologic disorder. The protein
            encoded by this gene contains (CAG)n repeats in the coding region,
            and the expansion of these repeats from the normal 12-44 to 52-86
            is one cause of Machado-Joseph disease. There is a negative
            correlation between the age of onset and CAG repeat numbers.
            Alternatively spliced transcript variants encoding different
            isoforms have been described for this gene. [provided by RefSeq,
            Jul 2016].
            
            Transcript Variant: This variant (reference, also known as variant
            1) encodes the longest isoform (reference isoform, also known as
            isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.113213.1,
                                           SRR11853564.9097.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000644486.2/ ENSP00000496695.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-440               DA827537.1         40-479
            441-1342            AB050194.1         304-1205
            1343-6884           AL049872.3         136144-141685       c
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.12"
     gene            1..6884
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /note="ataxin 3"
                     /db_xref="GeneID:4287"
                     /db_xref="HGNC:HGNC:7106"
                     /db_xref="MIM:607047"
     exon            1..54
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1116
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /EC_number="3.4.19.12"
                     /note="reference isoform is encoded by transcript variant
                     reference; josephin; spinocerebellar ataxia type 3
                     protein; olivopontocerebellar ataxia 3; Machado-Joseph
                     disease protein 1"
                     /codon_start=1
                     /product="ataxin-3 reference isoform"
                     /protein_id="NP_004984.2"
                     /db_xref="CCDS:CCDS9900.1"
                     /db_xref="GeneID:4287"
                     /db_xref="HGNC:HGNC:7106"
                     /db_xref="MIM:607047"
                     /translation="MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEE
                     RMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRL
                     RIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGY
                     SIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDG
                     SGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNL
                     TSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAM
                     SEEDMLQAAVTMSLETVRNDLKTEGKK"
     exon            55..219
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            220..264
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            265..350
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            351..417
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            418..505
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            506..638
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            639..805
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            806..902
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1021
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..6884
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1324..1329
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /note="hexamer: ATTAAA"
     polyA_site      1342
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /note="major polyA site"
     regulatory      1833..1838
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /note="hexamer: AATAAA"
     polyA_site      1854
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
     regulatory      6860..6865
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /note="hexamer: AATAAA"
     polyA_site      6884
                     /gene="ATXN3"
                     /gene_synonym="AT3; ATX3; JOS; MJD; MJD1; SCA3"
                     /note="major polyA site"
ORIGIN      
        1 gtgggggccg ttggctccag acaaataaac atggagtcca tcttccacga gaaacaagaa
       61 ggctcacttt gtgctcaaca ttgcctgaat aacttattgc aaggagaata ttttagccct
      121 gtggaattat cctcaattgc acatcagctg gatgaggagg agaggatgag aatggcagaa
      181 ggaggagtta ctagtgaaga ttatcgcacg tttttacagc agccttctgg aaatatggat
      241 gacagtggtt ttttctctat tcaggttata agcaatgcct tgaaagtttg gggtttagaa
      301 ctaatcctgt tcaacagtcc agagtatcag aggctcagga tcgatcctat aaatgaaaga
      361 tcatttatat gcaattataa ggaacactgg tttacagtta gaaaattagg aaaacagtgg
      421 tttaacttga attctctctt gacgggtcca gaattaatat cagatacata tcttgcactt
      481 ttcttggctc aattacaaca ggaaggttat tctatatttg tcgttaaggg tgatctgcca
      541 gattgcgaag ctgaccaact cctgcagatg attagggtcc aacagatgca tcgaccaaaa
      601 cttattggag aagaattagc acaactaaaa gagcaaagag tccataaaac agacctggaa
      661 cgagtgttag aagcaaatga tggctcagga atgttagacg aagatgagga ggatttgcag
      721 agggctctgg cactaagtcg ccaagaaatt gacatggaag atgaggaagc agatctccgc
      781 agggctattc agctaagtat gcaaggtagt tccagaaaca tatctcaaga tatgacacag
      841 acatcaggta caaatcttac ttcagaagag cttcggaaga gacgagaagc ctactttgaa
      901 aaacagcagc aaaagcagca acagcagcag cagcagcagc agcaggggga cctatcagga
      961 cagagttcac atccatgtga aaggccagcc accagttcag gagcacttgg gagtgatcta
     1021 ggtgatgcta tgagtgaaga agacatgctt caggcagctg tgaccatgtc tttagaaact
     1081 gtcagaaatg atttgaaaac agaaggaaaa aaataatacc tttaaaaaat aatttagata
     1141 ttcatacttt ccaacattat cctgtgtgat tacagcatag ggtccacttt ggtaatgtgt
     1201 caaagagatg aggaaataag acttttagcg gtttgcaaac aaaatgatgg gaaagtggaa
     1261 caatgcgtcg gttgtaggac taaataatga tcttccaaat attagccaaa gaggcattca
     1321 gcaattaaag acatttaaaa tagttttcta aatgtttctt tttctttttt gagtgtgcaa
     1381 tatgtaacat gtctaaagtt agggcatttt tcttggatct ttttgcagac tagctaatta
     1441 gctctcgcct caggcttttt ccatatagtt tgttttcttt ttctgtcttg taggtaagtt
     1501 ggctcacatc atgtaatagt ggctttcatt tcttattaac caaattaacc tttcaggaaa
     1561 gtatctctac tttcctgatg ttgataatag taatggttct agaaggatga acagttctcc
     1621 cttcaactgt ataccgtgtg ctccagtgtt ttcttgtgtt gttttctctg atcacaactt
     1681 ttctgctacc tggttttcat tattttccca caattctttt gaaagatggt aatcttttct
     1741 gaggtttagc gttttaagcc ctacgatggg atcattattt catgactggt gcgttcctaa
     1801 actctgaaat cagccttgca caagtacttg agaataaatg agcatttttt aaaatgtgtg
     1861 agcatgtgct ttcccagatg ctttatgaat gtcttttcac ttatatcaaa accttacagc
     1921 tttgttgcaa ccccttcttc ctgcgcctta ttttttcctt tcttctccaa ttgagaaaac
     1981 taggagaagc atagtatgca ggcaagtctc cttctgttag aagactaaac atacgtaccc
     2041 accatgaatg tatgatacat gaaatttggc cttcaatttt aatagcagtt ttattttatt
     2101 ttttctccta tgactggagc tttgtgttct ctttacagtt gagtcatgga atgtaggtgt
     2161 ctgcttcaca tcttttagta ggtatagctt gtcaaagatg gtgatctgga acatgaaaat
     2221 aatttactaa tgaaaatatg tttaaattta tactgtgatt tgacacttgc atcatgttta
     2281 gatagcttaa gaacaatgga agtcacagta cttagtggat ctataaataa gaaagtccat
     2341 agttttgata aatattctct ttaattgaga tgtacagaga gtttcttgct gggtcaatag
     2401 gatagtatca ttttggtgaa aaccatgtct ctgaaattga tgttttagtt tcagtgttcc
     2461 ctatccctca ttctccatct ccttttgaag ctcttttgaa tgttgaattg ttcataagct
     2521 aaaatccaag aaatttcagc tgacaacttc gaaaattata atatggtata ttgccctcct
     2581 ggtgtgtggc tgcacacatt ttatcaggga aagttttttg atctaggatt tattgctaac
     2641 taactgaaaa gagaagaaaa aatatctttt atttatgatt ataaaatagc tttttcttcg
     2701 atataacaga ttttttaagt cattattttg tgccaatcag ttttctgaag tttcccttac
     2761 acaaaaggat agctttattt taaaatctaa agtttctttt aatagttaaa aatgtttcag
     2821 aagaattata aaactttaaa actgcaaggg atgttggagt ttagtactac tccctcaaga
     2881 tttaaaaagc taaatatttt aagactgaac atttatgtta attattacca gtgtgtttgt
     2941 catattttcc atggatattt gttcattacc tttttccatt gaaaagttac attaaacttt
     3001 tcatacactt gaattgatga gctacctaat ataaaaatga gaaaaccaat atgcatttta
     3061 aagttttaac tttagagttt ataaagttca tatataccct agttaaagca cttaagaaaa
     3121 tatggcatgt ttgactttta gttcctagag agtttttgtt tttgtttttg tttttttttg
     3181 agacggagtc ttgctatgtc tcccaggctg gagggcagtg gcatgatctc ggctcactac
     3241 aacttccacc tcccgggttc aagcaattct cctgcctcag cctccagagt agctgggatt
     3301 acaggcgccc accaccacac ccggcagatt tttgtatttt tggtagagac gcggtttcat
     3361 catgtttggc caggctggtc tcgaactcct gacctcaggt gatccgcctg ccttggcctc
     3421 ccaaagtgtt gggattacag gcatgagcca ctgcgcctgg ccagctagag agtttttaaa
     3481 gcagagctga gcacacactg gatgcgtttg aatgtgtttg tgtagtttgt tgtgaaattg
     3541 ttacatttag caggcagatc cagaagcact agtgaactgt catcttggtg gggttggctt
     3601 aaatttaatt gactgtttag attccatttc ttaattgatt ggccagtatg aaaagatgcc
     3661 agtgcaagta accatagtat caaaaaagtt aaaaattatt caaagctata gtttatacat
     3721 caggtactgc catttactgt aaaccacctg caagaaagtc aggaacaact aaattcacaa
     3781 gaactgtcct gctaagaagt gtattaaaga tttccatttt gttttactaa ttgggaacat
     3841 cttaatgttt aatatttaaa ctattggtat catttttcta atgtataatt tgtattactg
     3901 ggatcaagta tgtacagtgg tgatgctagt agaagtttaa gccttggaaa taccactttc
     3961 atattttcag atgtcatgga tttaatgagt aatttatgtt tttaaaattc agaatagtta
     4021 atctctgatc taaaaccatc aatctatgtt ttttacggta atcatgtaaa tatttcagta
     4081 atataaactg tttgaaaagg ctgctgcagg taaactctat actaggatct tggccaaata
     4141 atttacaatt cacagaatat tttatttaag gtggtgcttt ttttttttgt ccttaaaact
     4201 tgatttttct taactttatt catgatgcca aagtaaatga ggaaaaaaac tcaaaaccag
     4261 ttgagtatca ttgcagacaa aactaccagt agtccatatt gtttaatatt aagttgaata
     4321 aaataaattt tatttcagtc agagcctaaa tcacattttg attgtctgaa tttttgatac
     4381 tatttttaaa atcatgctag tggcggctgg gcgtggtagc tcacgcctgt aatcccagca
     4441 ttttgggagg ccgaagtggg tggatcacga ggtcgggagt tcgagaccag cttggccaaa
     4501 atggtgaaac cccatctgta ctaaaaacta caaaaattag ctgggcgcgg tggcaggtgc
     4561 ctgtaatccc agctacctgg gagtctgagg caggagaatt gcttgaaccc tggcgacaga
     4621 ggatgcagtg agccaagatg gtgccactgt actccagact gggcgacaga gtgagactct
     4681 gtctcaaaaa aaaaaaaaaa atcatgctag tgccaagagc tactaaattc ttaaaaccgg
     4741 cccattggac ctgtacagat aaaaaataga ttcagtgcat aatcaaaata tgataatttt
     4801 aaaatcttaa gtagaaaaat aaatcttgat gttttaaatt cttacgagga ttcaatagtt
     4861 aatattgatg atctcccggc tgggtgcagt ggctcacgcc tgtaatccca gcagttctgg
     4921 aggctgaggt gggcgaatca cttcaggcca ggagttcaag accagtctgg gcaacatggt
     4981 gaaacctcgt ttctactaaa aatacaaaaa ttagccgggc gtggttgcac acacttgtaa
     5041 tcccagctac tcaggaggct aagaatcgca tgagcctagg aggcagaggt tgcagagtgc
     5101 caagggctca ccactgcatt ccagcctgcc caacagagtg agacactgtt tctgaaaaaa
     5161 aaaaatatat atatatatat atatatgtgt gtatatatat atgtatatat atatgacttc
     5221 ctattaaaaa ctttatccca gtcgggggca gtggctcacg cctgtaatcc caacactttg
     5281 ggaggctgag gcaggtggat cacctgaagt ccggagtttg agaccagcct ggccaacatg
     5341 gtgaaacccc atctctacta aaaatacaaa acttaagcca ggtatggtgg cgggcacctg
     5401 taatcccagt tacttgggag gctgaggcag gagaatcgtt taaacccagg aggtggaggt
     5461 tgcagtgagc tgagatcgtg ccattgcact ctagcctggg caacaagagt aaaactccat
     5521 cttaaaggtt tgtttgtttt tttttaatcc ggaaacgaag aggcgttggg ccgctatttt
     5581 ctttttcttt ctttctttct ttcttttttt ttttttctga gacggagtct agctctgctg
     5641 cccaggctgg agtacaatga cacgatgttg gctcactgca acctccacct cctgggttca
     5701 agcgattctc ctgcctcagc ctcccaagta cctgggatta caggcacctg ccactacacc
     5761 tggcgaatat ttgttttttt tagtagagac gggcttttac catgttaggc tggtctcaaa
     5821 ctcctgacct caggtgatct gcctgccttg gcctcccaaa gtgctgggat tacaggtgca
     5881 ggccaccaca cccggccttg ggccactgtt ttcaaagtga attgtttgtt gtatcgagtc
     5941 cttaagtatg gatatatatg tgaccctaat taagaactac cagattggat caactaatca
     6001 tgtcagcaat gtaaataact ttatttttca tattcaaaat aaaaactttc ttttatttct
     6061 ggccccttta taaccagcat ctttttgctt taaaaaatga cctggctttg tattttttta
     6121 gtcttaaaca taataaaaat atttttgttc taatttgctt tcatgagtga agattattga
     6181 catcgttggt aaattctaga attttgattt tgttttttaa tttgaagaaa atctttgcta
     6241 ttattatttt ttccaagtgg tctggcattt taagaattag tgctaataac gtaacttcta
     6301 aatttgtcgt aattggcatg tttaatagca tatcaaaaaa cattttaagc ctgtggattc
     6361 atagacaaag caatgagaaa cattagtaaa atataaatgg atattcctga tgcatttagg
     6421 aagctctcaa ttgtctcttg catagttcaa ggaatgtttt ctgaattttt ttaatgcttt
     6481 tttttttttt gaaagaggaa aacatacatt tttaaatgtg attatctaat ttttacaaca
     6541 ctgggctatt aggaataact ttttaaaaat tactgttctg tataaatatt tgaaattcaa
     6601 gtacagaaaa tatctgaaac aaaaagcatt gttgtttggc catgatacaa gtgcactgtg
     6661 gcagtgccgc ttgctcagga cccagccctg cagcccttct gtgtgtgctc cctcgttaag
     6721 ttcatttgct gttattacac acacaggcct tcctgtctgg tcgttagaaa agccgggctt
     6781 ccaaagcact gttgaacaca ggattctgtt gttagtgtgg atgttcaatg agttgtattt
     6841 taaatatcaa agattattaa ataaagataa tgtttgcttt tcta
//
