LOCUS       NM_001128164           10557 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens ataxin 1 (ATXN1), transcript variant 2, mRNA.
ACCESSION   NM_001128164
VERSION     NM_001128164.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Sheeler,C., Labrada,E., Duvick,L., Thompson,L.M., Zhang,Y.,
            Orr,H.T. and Cvetanovic,M.
  TITLE     Expanded ATXN1 alters transcription and calcium signaling in SCA1
            human motor neurons differentiated from induced pluripotent stem
            cells
  JOURNAL   Neurobiol Dis 201, 106673 (2024)
   PUBMED   39307401
  REMARK    GeneRIF: Expanded ATXN1 alters transcription and calcium signaling
            in SCA1 human motor neurons differentiated from induced pluripotent
            stem cells.
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   3  (bases 1 to 10557)
  AUTHORS   Lou,L.Q., Zhou,W.Q., Song,X. and Chen,Z.
  TITLE     Elevation of hsa-miR-7-5p level mediated by CtBP1-p300-AP1 complex
            targets ATXN1 to trigger NF-kappaB-dependent inflammation response
  JOURNAL   J Mol Med (Berl) 101 (3), 223-235 (2023)
   PUBMED   36629882
  REMARK    GeneRIF: Elevation of hsa-miR-7-5p level mediated by CtBP1-p300-AP1
            complex targets ATXN1 to trigger NF-kappaB-dependent inflammation
            response.
REFERENCE   4  (bases 1 to 10557)
  AUTHORS   Tammara,V. and Das,A.
  TITLE     Governing dynamics and preferential binding of the AXH domain
            influence the aggregation pathway of Ataxin-1
  JOURNAL   Proteins 91 (3), 380-394 (2023)
   PUBMED   36208132
  REMARK    GeneRIF: Governing dynamics and preferential binding of the AXH
            domain influence the aggregation pathway of Ataxin-1.
REFERENCE   5  (bases 1 to 10557)
  AUTHORS   Bergeron,D., Lapointe,C., Bissonnette,C., Tremblay,G., Motard,J.
            and Roucou,X.
  TITLE     An out-of-frame overlapping reading frame in the ataxin-1 coding
            sequence encodes a novel ataxin-1 interacting protein
  JOURNAL   J Biol Chem 288 (30), 21824-21835 (2013)
   PUBMED   23760502
  REMARK    GeneRIF: Data indicate that the alternative ataxin-1 (ATXN1)
            protein is constitutively co-expressed and interacts with ATXN1.
REFERENCE   6  (bases 1 to 10557)
  AUTHORS   Servadio,A., Koshy,B., Armstrong,D., Antalffy,B., Orr,H.T. and
            Zoghbi,H.Y.
  TITLE     Expression analysis of the ataxin-1 protein in tissues from normal
            and spinocerebellar ataxia type 1 individuals
  JOURNAL   Nat Genet 10 (1), 94-98 (1995)
   PUBMED   7647801
REFERENCE   7  (bases 1 to 10557)
  AUTHORS   Banfi,S., Servadio,A., Chung,M.Y., Kwiatkowski,T.J. Jr.,
            McCall,A.E., Duvick,L.A., Shen,Y., Roth,E.J., Orr,H.T. and
            Zoghbi,H.Y.
  TITLE     Identification and characterization of the gene causing type 1
            spinocerebellar ataxia
  JOURNAL   Nat Genet 7 (4), 513-520 (1994)
   PUBMED   7951322
REFERENCE   8  (bases 1 to 10557)
  AUTHORS   Opal,P. and Ashizawa,T.
  TITLE     Spinocerebellar Ataxia Type 1
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301363
REFERENCE   9  (bases 1 to 10557)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
REFERENCE   10 (bases 1 to 10557)
  AUTHORS   Volz,A., Fonatsch,C. and Ziegler,A.
  TITLE     Regional mapping of the gene for autosomal dominant spinocerebellar
            ataxia (SCA1) by localizing the closely linked D6S89 locus to
            6p24.2----p23.05
  JOURNAL   Cytogenet Cell Genet 60 (1), 37-39 (1992)
   PUBMED   1582256
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC011026.1, BP365416.1,
            X79204.1 and AL009031.1.
            
            On Feb 25, 2020 this sequence version replaced NM_001128164.1.
            
            Summary: The autosomal dominant cerebellar ataxias (ADCA) are a
            heterogeneous group of neurodegenerative disorders characterized by
            progressive degeneration of the cerebellum, brain stem and spinal
            cord. Clinically, ADCA has been divided into three groups: ADCA
            types I-III. ADCAI is genetically heterogeneous, with five genetic
            loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6,
            being assigned to five different chromosomes. ADCAII, which always
            presents with retinal degeneration (SCA7), and ADCAIII often
            referred to as the `pure' cerebellar syndrome (SCA5), are most
            likely homogeneous disorders. Several SCA genes have been cloned
            and shown to contain CAG repeats in their coding regions. ADCA is
            caused by the expansion of the CAG repeats, producing an elongated
            polyglutamine tract in the corresponding protein. The expanded
            repeats are variable in size and unstable, usually increasing in
            size when transmitted to successive generations. The function of
            the ataxins is not known. This locus has been mapped to chromosome
            6, and it has been determined that the diseased allele contains
            40-83 CAG repeats, compared to 6-39 in the normal allele, and is
            associated with spinocerebellar ataxia type 1 (SCA1). Alternative
            splicing results in multiple transcript variants, with one variant
            encoding multiple distinct proteins, ATXN1 and Alt-ATXN1, due to
            the use of overlapping alternate reading frames. [provided by
            RefSeq, Nov 2017].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. This variant encodes the same protein represented by
            the longer protein encoded by variant 1, ATXN1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038192.2799468.1,
                                           SRR14038194.3861132.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000436367.6/ ENSP00000416360.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 BC011026.1         34-34
            2-581               BP365416.1         3-582
            582-1525            X79204.1           625-1568
            1526-3695           X79204.1           1572-3741
            3696-10557          AL009031.1         93902-100763
FEATURES             Location/Qualifiers
     source          1..10557
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p22.3"
     gene            1..10557
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /note="ataxin 1"
                     /db_xref="GeneID:6310"
                     /db_xref="HGNC:HGNC:10548"
                     /db_xref="MIM:601556"
     exon            1..163
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            164..278
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            279..404
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            405..532
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            533..594
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            595..732
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     exon            733..2809
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             893..3340
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /note="isoform ATXN1 is encoded by transcript variant 2;
                     alternative ataxin1; spinocerebellar ataxia type 1
                     protein"
                     /codon_start=1
                     /product="ataxin-1 isoform ATXN1"
                     /protein_id="NP_001121636.1"
                     /db_xref="CCDS:CCDS34342.1"
                     /db_xref="GeneID:6310"
                     /db_xref="HGNC:HGNC:10548"
                     /db_xref="MIM:601556"
                     /translation="MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTA
                     WLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTL
                     PAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVA
                     SAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQ
                     QQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTM
                     IPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRY
                     GAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPA
                     ADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPA
                     TAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAI
                     VTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAA
                     PPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSP
                     GVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVC
                     ISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSE
                     NGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSL
                     IPQEVKICIEGRSNVGK"
     exon            2810..10557
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10534..10539
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /note="hexamer: AATAAA"
     polyA_site      10557
                     /gene="ATXN1"
                     /gene_synonym="ATX1; D6S504E; SCA1"
                     /note="major polyA site"
ORIGIN      
        1 atttctacta cagtggcgga gccgtacagg acctgtttca ctgcaggggg atccaaaaca
       61 agccccgtgg agcagcagcc agagcaacag cagccgcaag acattgtttc tctccctctg
      121 cccccccttc cccacgcaac cccagatcca tttacacttt acagagcatc gtgcatcaag
      181 tcaccagggt ggtccattca agctgcagat ttgtttgtca tccttgtaca gcaatctcct
      241 cctccactgc cactacaggg aagtgcatca catgtcagca tactggagca tagtgaaaga
      301 gtctattttg aagcttcaaa cttagtgctg ctgcagacca ggaacaagag agaaagagtg
      361 gatttcagcc tgcacggatg gtcttgaaac acaaatggtt tttggtctag gcgttttaca
      421 ctgagattct ccactgccac cctttctact caagcaaaat cttcgtgaaa agatctgctg
      481 caaggaactg atagcttatg gttctccatt gtgatgaaag cacatggtac agttttccaa
      541 agaaattaga ccattttctt cgtgagaaag aaatcgacgt gctgttttca tagggtattt
      601 ctcacttctc tgtgaaagga agaaagaaca cgcctgagcc caagagccct caggagccct
      661 ccagagcctg tgggaagtct ccatggtgaa gtataggctg aggctacctg tgaacagtac
      721 gcagtgaatg ttcatccaga gctgctgttg gcggattgta cccacgggga gatgattcct
      781 catgaagagc ctggatcccc tacagaaatc aaatgtgact ttccgtttat cagactaaaa
      841 tcagagccat ccagacagtg aaacagtcac cgtggagggg ggacggcgaa aaatgaaatc
      901 caaccaagag cggagcaacg aatgcctgcc tcccaagaag cgcgagatcc ccgccaccag
      961 ccggtcctcc gaggagaagg cccctaccct gcccagcgac aaccaccggg tggagggcac
     1021 agcatggctc ccgggcaacc ctggtggccg gggccacggg ggcgggaggc atgggccggc
     1081 agggacctcg gtggagcttg gtttacaaca gggaataggt ttacacaaag cattgtccac
     1141 agggctggac tactccccgc ccagcgctcc caggtctgtc cccgtggcca ccacgctgcc
     1201 tgccgcgtac gccaccccgc agccagggac cccggtgtcc cccgtgcagt acgctcacct
     1261 gccgcacacc ttccagttca ttgggtcctc ccaatacagt ggaacctatg ccagcttcat
     1321 cccatcacag ctgatccccc caaccgccaa ccccgtcacc agtgcagtgg cctcggccgc
     1381 aggggccacc actccatccc agcgctccca gctggaggcc tattccactc tgctggccaa
     1441 catgggcagt ctgagccaga cgccgggaca caaggctgag cagcagcagc agcagcagca
     1501 gcagcagcag cagcagcatc agcatcagca gcagcagcag cagcagcagc agcagcagca
     1561 gcagcagcac ctcagcaggg ctccggggct catcaccccg gggtcccccc caccagccca
     1621 gcagaaccag tacgtccaca tttccagttc tccgcagaac accggccgca ccgcctctcc
     1681 tccggccatc cccgtccacc tccaccccca ccagacgatg atcccacaca cgctcaccct
     1741 ggggcccccc tcccaggtcg tcatgcaata cgccgactcc ggcagccact ttgtccctcg
     1801 ggaggccacc aagaaagctg agagcagccg gctgcagcag gccatccagg ccaaggaggt
     1861 cctgaacggt gagatggaga agagccggcg gtacggggcc ccgtcctcag ccgacctggg
     1921 cctgggcaag gcaggcggca agtcggttcc tcacccgtac gagtccaggc acgtggtggt
     1981 ccacccgagc ccctcagact acagcagtcg tgatccttcg ggggtccggg cctctgtgat
     2041 ggtcctgccc aacagcaaca cgcccgcagc tgacctggag gtgcaacagg ccactcatcg
     2101 tgaagcctcc ccttctaccc tcaacgacaa aagtggcctg catttaggga agcctggcca
     2161 ccggtcctac gcgctctcac cccacacggt cattcagacc acacacagtg cttcagagcc
     2221 actcccggtg ggactgccag ccacggcctt ctacgcaggg actcaacccc ctgtcatcgg
     2281 ctacctgagc ggccagcagc aagcaatcac ctacgccggc agcctgcccc agcacctggt
     2341 gatccccggc acacagcccc tgctcatccc ggtcggcagc actgacatgg aagcgtcggg
     2401 ggcagccccg gccatagtca cgtcatcccc ccagtttgct gcagtgcctc acacgttcgt
     2461 caccaccgcc cttcccaaga gcgagaactt caaccctgag gccctggtca cccaggccgc
     2521 ctacccagcc atggtgcagg cccagatcca cctgcctgtg gtgcagtccg tggcctcccc
     2581 ggcggcggct ccccctacgc tgcctcccta cttcatgaaa ggctccatca tccagttggc
     2641 caacggggag ctaaagaagg tggaagactt aaaaacagaa gatttcatcc agagtgcaga
     2701 gataagcaac gacctgaaga tcgactccag caccgtagag aggattgaag acagccatag
     2761 cccgggcgtg gccgtgatac agttcgccgt cggggagcac cgagcccagg tcagcgttga
     2821 agttttggta gagtatcctt tttttgtgtt tggacagggc tggtcatcct gctgtccgga
     2881 gagaaccagc cagctctttg atttgccgtg ttccaaactc tcagttgggg atgtctgcat
     2941 ctcgcttacc ctcaagaacc tgaagaacgg ctctgttaaa aagggccagc ccgtggatcc
     3001 cgccagcgtc ctgctgaagc actcaaaggc cgacggcctg gcgggcagca gacacaggta
     3061 tgccgagcag gaaaacggaa tcaaccaggg gagtgcccag atgctctctg agaatggcga
     3121 actgaagttt ccagagaaaa tgggattgcc tgcagcgccc ttcctcacca aaatagaacc
     3181 cagcaagccc gcggcaacga ggaagaggag gtggtcggcg ccagagagcc gcaaactgga
     3241 gaagtcagaa gacgaaccac ctttgactct tcctaagcct tctctaattc ctcaggaggt
     3301 taagatttgc attgaaggcc ggtctaatgt aggcaagtag aggcagcgtg ggggaaagga
     3361 aacgtggctc tcccttatca tttgtatcca gattactgta ctgtaggcta aaataacaca
     3421 gtatttacat gttatcttct taattttagg tttctgttct aaccttgtca ttagagttac
     3481 agcaggtgtg tcgcaggaga ctggtgcata tgctttttcc acgagtgtct gtcagtgagc
     3541 gggcgggagg aagggcacag caggagcggt cagggctcca ggcatccccg gggaagaaag
     3601 gaacggggct tcacagtgcc tgccttctct agcggcacag aagcagccgg gggcgctgac
     3661 tcccgctagt gtcaggagaa aagtcccgtg ggaagggtcc tgcaggggtg cagggttgca
     3721 cgcatgtggg ggtgcacagg cgctgtggcg gcgagtgagg gtctcttttt ctctgcctcc
     3781 ctctgcctca ctctcttgct atcggcatgg gccggggggg ttcagagcag tgtcctcctg
     3841 gggttcccac gtgcaaaatc aacatcagga acccagcttc agggcatcgc ggagacgcgt
     3901 cagatggcag atttggaaag ttaaccattt aaaagaacat ttttctctcc aacatatttt
     3961 acaataaaag caacttttaa ttgtatagat atatatttcc ccctatgggg cctgactgca
     4021 ctgatatata ttttttttaa agagcaactg ccacatgcgg gatttcattt ctgcttttta
     4081 ctagtgcagc gatgtcacca gggtgttgtg gtggacaggg aagcccctgc tgtcatggcc
     4141 ccacatgggg taaggggggt tgggggtggg ggagagggag agagcgaaca cccacgctgg
     4201 tttctgtgca gtgttaggaa aaccaatcag gttattgcat tgacttcact cccaagaggt
     4261 agatgcaaac tgcccttcag tgagagcaac agaagctctt cacgttgagt ttgcgaaatc
     4321 tttttgtctt tgaactctag tactgtttat agttcatgac tatggacaac tcgggtgcca
     4381 cttttttttt ttttcagatt ccagtgtgac atgaggaatt agattttgaa gatgagcata
     4441 tattactatc tttaagcatt taaaaatact gttcacactt tattaccaag catcttggtc
     4501 tctcattcaa caagtactgt atctcacttt aaactctttg gggaaaaaac aaaaacaaaa
     4561 aaaactaagt tgctttcttt ttttcaacac tgtaactaca tttcagctct gcagaattgc
     4621 tgaagagcaa gatattgaaa gtttcaatgt ggtttaaagg gatgaatgtg aattatgaac
     4681 tagtatgtga caataaatga ccaccaagta ctacctgacg ggaggcactt ttcactttga
     4741 tgtctgagaa tcagttcaag gcatatgcag agttggcaga gaaactgaga gaaaagggat
     4801 ggagaagaga atactcattt ttgtccagtg tttttctttt taagatgaac ttttaaagaa
     4861 ccttgcgatt tgcacatatt gagtttataa cttgtgtgat attcctgcag tttttatcca
     4921 ataacattgt gggaaaggtt tgggggactg aacgagcata aataaatgta gcaaaatttc
     4981 tttctaacct gcctaaactc taggccattt tataaggtta tgttcctttg aaaattcatt
     5041 ttggtctttt taccacatct gtcacaaaaa gccaggtctt agcgggctct tagaaactct
     5101 gagaattttc ttcagattca ttgagagagt tttccataaa gacatttata tatgtgagca
     5161 agattttttt taaacaatta ctttattatt gttgttatta atgttatttt cagaatggct
     5221 tttttttttc tattcaaaat caaatcgaga tttaatgttt ggtacaaacc cagaaagggt
     5281 atttcatagt ttttaaacct ttcattccca gagatccgaa atatcatttg tgggttttga
     5341 atgcatcttt aaagtgcttt aaaaaaaagt tttataagta gggagaaatt tttaaatatt
     5401 cttacttgga tggctgcaac taaactgaac aaatacctga cttttctttt accccattga
     5461 aaatagtact ttcttcgttt cacaaattaa aaaaaaaatc tggtatcaac ccacattttg
     5521 gctgtctagt attcatttac atttagggtt caccaggact aatgattttt ataaaccgtt
     5581 ttctggggtg taccaaaaac atttgaatag gtttagaata gctagaatag ttccttgact
     5641 ttcctcgaat ttcattaccc tctcagcatg cttgcagaga gctgggtggg ctcattcttg
     5701 cagtcatact gcttatttag tgctgtattt tttaaacgtt tctgttcaga gaacttgctt
     5761 aatcttccat atattctgct cagggcactt gcaattatta ggttttgttt ttctttttgt
     5821 tttttagcct ttgatggtaa gaggaatacg ggctgccaca tagactttgt tctcattaat
     5881 atcactattt acaactcatg tggactcaga aaaacacaca ccaccttttg gcttacttcg
     5941 agtattgaat tgactggatc cactaaacca acactaagat gggaaaacac acatggtttg
     6001 gagcaatagg aacatcatca taatttttgt ggttctattt caggtatagg aattataaaa
     6061 taattggttc tttctaaaca cttgtcccat ttcattctct tgctttttta gcatgtgcaa
     6121 tactttctgt gccaatagag tctgaccagt gtgctatata gttaaagctc attccctttt
     6181 ggctttttcc ttgtttggtt gatcttcccc attctggcca gagcagggct ggagggaagg
     6241 agccaggagg gagagagcct cccacctttc ccctgctgcg gatgctgagt gctggggcgg
     6301 ggagccttca ggagccccgt gcgtctgccg ccacgttgca gaaagagcca gccaaggaga
     6361 cccgggggag gaaccgcagt gtcccctgtc accacacgga atagtgaatg tggagtgtgg
     6421 agaggaagga ggcagattca tttctaagac gcactctgga gccatgtagc ctggagtcaa
     6481 cccattttcc acggtctttt ctgcaagtgg gcaggcccct cctcggggtc tgtgtccttg
     6541 agacttggag ccctgcctct gagcctggac gggaagtgtg gcctgttgtg tgtgtgcgtt
     6601 ctgagcgtgt tggccagtgg ctgtggaggg gaccacctgc cacccacggt caccactccc
     6661 ttgtggcagc tttctcttca aataggaaga acgcacagag ggcaggagcc tcctgtttgc
     6721 agacgttggc gggccccgag gctcccagag cagcctctgt caccgcttct gtgtagcaaa
     6781 cattaacgat gacaggggta gaaattcttc ggtgccgttc agcttacaag gatcagccat
     6841 gtgcctctgt actatgtcca ctttgcaata tttaccgaca gccgtctttt gttctttctt
     6901 tcctgttttc catttttaaa ctagtaacag caggcctttt gcgtttacaa tggaacacaa
     6961 tcaccaagaa attagtcagg gcgaaaagaa aaaaataata ctattaataa gaaaccaaca
     7021 aacaagaacc tctctttcta gggatttcta aatatataaa atgactgttc cttagaatgt
     7081 ttaacttaag aattatttca gtttgtctgg gccacactgg ggcagagggg ggagggaggg
     7141 atacagagat ggatgccact tacctcagat cttttaaagt ggaaatccaa attgaatttt
     7201 catttggact ttcaggataa ttttctatgt tggtcaactt ttcgttttcc ctaactcacc
     7261 cagtttagtt tgggatgatt tgatttctgt tgttgttgat cccatttcta acttggaatt
     7321 gtgagcctct atgttttctg ttaggtgagt gtgttgggtt ttttcccccc accaggaagt
     7381 ggcagcatcc ctccttctcc cctaaaggga ctctgcggaa cctttcacac ctctttctca
     7441 gggacggggc aggtgtgtgt gtggtacact gacgtgtcca gaagcagcac tttgactgct
     7501 ctggagtagg gttgtacaat ttcaaggaat gtttggattt cctgcatctt gtggattact
     7561 ccttagatac cgcatagatt gcaatataat gctgcatgtt caagatgaac agtagctcct
     7621 agtaatcata aaatccactc tttgcacagt ttgatcttta ctgaaatatg ttgccaaaat
     7681 ttatttttgt tgttgtagct ctggattttg ttttgttttg ttttttaagg aaacgattga
     7741 caataccctt taacatctgt gactactaag gaaacctatt tctttcatag agagaaaaat
     7801 ctccaatgct tttgaagaca ctaataccgt gctatttcag atatgggtga ggaagcagag
     7861 ctctcggtac cgaaggccgg gcttcttgag ctgtgttggt tgtcatggct actgtttcat
     7921 gaaccacaag cagctcaaca gactggtctg ttgccttctg aaaccctttg cacttcaatt
     7981 tgcaccaggt gaaaacaggg ccagcagact ccatggccca attcggtttc ttcggtggtg
     8041 atgtgaaagg agagaattac actttttttt tttttaagtg gcgtggaggc ctttgcttcc
     8101 acatttgttt ttaacccaga atttctgaaa tagagaattt aagaacacat caagtaataa
     8161 atatacagag aatatacttt tttataaagc acatgcatct gctattgtgt tgggttggtt
     8221 tcctctcttt tccacggaca gtgttgtgtt tctggcatag ggaaactcca aacaacttgc
     8281 acacctctac tccggagctg agatttcttt tacatagatg acctcgcttc aaatacgtta
     8341 ccttactgat gataggatct tttcttgtag cactatacct tgtgggaatt tttttttaaa
     8401 tgtacacctg atttgagaag ctgaagaaaa caaaattttg aagcactcac tttgaggagt
     8461 acaggtaatg ttttaaaaaa ttgcacaaaa gaaaaatgaa tgtcgaaatg attcattcag
     8521 tgtttgaaag atatggctct gttgaaacaa tgagtttcat actttgtttg taaaaaaaaa
     8581 aaagcagaga agggttgaaa gttacatgtt tttttgtata tagaaatttg tcatgtctaa
     8641 atgatcagat ttgtatggtt atggcctgga agaattacta cgtaaaaggc tcttaaacta
     8701 tacctatgct tattgttatt tttgttacat atagccctcg tctgagggag gggaactcgg
     8761 tattctgcga tttgagaata ctgttcattc ctatgctgaa agtacttctc tgagctccct
     8821 tcttagtcta aactcttaag ccattgcaac ttctttttct tcagagatga tgtttgacat
     8881 tttcagcact tcctgttcct ataaacccaa agaatataat cttgaacacg aagtgtttgt
     8941 aacaagggat ccaggctacc aatcaaacag gactcattat ggggacaaaa aaaaaaatta
     9001 tttcaccttc tttcccccca cacctcattt aaatgggggg agtaaaaaca tgatttcaat
     9061 gtaaatgcct cattttattt tagttttatt ttgattttta tttaatataa agaggccaga
     9121 ataaatacgg agcatcttct cagaatagta ttcctgtcca aaaatcaagc cggacagtgg
     9181 aaactggaca gctgtgggga tattaagcac ccccacttac aattcttaaa ttcagaatct
     9241 cgtcccctcc cttctcgttg aaggcaactg ttctggtagc taactttctc ctgtgtaatg
     9301 gcgggaggga acaccggctt cagtttttca tgtccccatg acttgcatac aaatggttca
     9361 actgtattaa aattaagtgc atttggccaa taggtagtat ctatacaata acaacaatct
     9421 ctaagaattt ccataacttt tcttatctga aaggactcaa gtcttccact gcagatacat
     9481 tggaggcttc acccacgttt tctttccctt tagtttgttt gctgtctgga tggccaatga
     9541 gcctgtctcc ttttctgtgg ccaatctgaa ggccttcgtt ggaagtgttg tttacagtaa
     9601 tccttaccaa gataacatac tgtcctccag aataccaagt attaggtgac actagctcaa
     9661 gctgttgtct tcagagcagt taccaagaag ctcggtgcac aggttttctc tggttcttac
     9721 aggaaccacc tactctttca gttttctggc ccaggagtgg ggtaaatcct ttagttagtg
     9781 catttgaact tgatacctgt gcattcagtt ctgtgaatac tgcccttttt ggcggggttt
     9841 cctcatctcc ccagcctgaa ctgctcaact ctaaacccaa attagtgtca gccgaaagga
     9901 ggtttcaaga tagtcctgtc agtatttgtg gtgaccttca gattagacag tcttcatttc
     9961 cagccagtgg agtcctggct ccagagccat ctctgagact cgtactactg gatgttttaa
    10021 tatcagatca ttacccacca tatgcctccc acaggccaag ggaaaacaga caccagaact
    10081 tgggttgagg gcactaccag actgacatgg ccagtacaga ggagaactag ggaaggaatg
    10141 atgttttgca ccttattgaa aagaaaattt taagtgcata cataatagtt aagagctttt
    10201 attgtgacag gagaactttt ttccatatgc gtgcatactc tctgtaattc cagtgtaaaa
    10261 tattgtactt gcactagctt ttttaaacaa atattaaaaa atggaagaat tcatattcta
    10321 ttttctaatc gtggtgtgtc tatttgtagg atacactcga gtctgtttat tgaattttat
    10381 ggtccctttc tttgatggtg cttgcaggtt ttctaggtag aaattatttc attattataa
    10441 taaaacaatg tttgattcaa aatttgaaca aaattgtttt aaataaattg tctgtatacc
    10501 agtacaagtt tattgtttca gtatactcgt actaataaaa taacagtgcc aattgca
//
