LOCUS       NM_001374385            6161 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens ATPase phospholipid transporting 8B1 (ATP8B1),
            transcript variant 1, mRNA.
ACCESSION   NM_001374385 XM_006722481
VERSION     NM_001374385.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Dieudonne,T., Kummerer,F., Laursen,M.J., Stock,C., Flygaard,R.K.,
            Khalid,S., Lenoir,G., Lyons,J.A., Lindorff-Larsen,K. and Nissen,P.
  TITLE     Activation and substrate specificity of the human P4-ATPase ATP8B1
  JOURNAL   Nat Commun 14 (1), 7492 (2023)
   PUBMED   37980352
  REMARK    GeneRIF: Activation and substrate specificity of the human
            P4-ATPase ATP8B1.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Chen,L.C., Huang,S.P., Shih,C.T., Li,C.Y., Chen,Y.T., Huang,C.Y.,
            Yu,C.C., Lin,V.C., Lee,C.H., Geng,J.H. and Bao,B.Y.
  TITLE     ATP8B1: A prognostic prostate cancer biomarker identified via
            genetic analysis
  JOURNAL   Prostate 83 (6), 602-611 (2023)
   PUBMED   36794287
  REMARK    GeneRIF: ATP8B1: A prognostic prostate cancer biomarker identified
            via genetic analysis.
REFERENCE   3  (bases 1 to 6161)
  AUTHORS   Gomez-Mellado,V.E., Chang,J.C., Ho-Mok,K.S., Bernardino
            Morcillo,C., Kersten,R.H.J., Oude Elferink,R.P.J., Verhoeven,A.J.
            and Paulusma,C.C.
  TITLE     ATP8B1 Deficiency Results in Elevated Mitochondrial
            Phosphatidylethanolamine Levels and Increased Mitochondrial
            Oxidative Phosphorylation in Human Hepatoma Cells
  JOURNAL   Int J Mol Sci 23 (20), 12344 (2022)
   PUBMED   36293199
  REMARK    GeneRIF: ATP8B1 Deficiency Results in Elevated Mitochondrial
            Phosphatidylethanolamine Levels and Increased Mitochondrial
            Oxidative Phosphorylation in Human Hepatoma Cells.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6161)
  AUTHORS   Nayagam,J.S., Foskett,P., Strautnieks,S., Agarwal,K., Miquel,R.,
            Joshi,D. and Thompson,R.J.
  TITLE     Clinical phenotype of adult-onset liver disease in patients with
            variants in ABCB4, ABCB11, and ATP8B1
  JOURNAL   Hepatol Commun 6 (10), 2654-2664 (2022)
   PUBMED   35894240
  REMARK    GeneRIF: Clinical phenotype of adult-onset liver disease in
            patients with variants in ABCB4, ABCB11, and ATP8B1.
REFERENCE   5  (bases 1 to 6161)
  AUTHORS   Bull,L.N., van Eijk,M.J., Pawlikowska,L., DeYoung,J.A., Juijn,J.A.,
            Liao,M., Klomp,L.W., Lomri,N., Berger,R., Scharschmidt,B.F.,
            Knisely,A.S., Houwen,R.H. and Freimer,N.B.
  TITLE     A gene encoding a P-type ATPase mutated in two forms of hereditary
            cholestasis
  JOURNAL   Nat Genet 18 (3), 219-224 (1998)
   PUBMED   9500542
REFERENCE   6  (bases 1 to 6161)
  AUTHORS   Carlton,V.E., Knisely,A.S. and Freimer,N.B.
  TITLE     Mapping of a locus for progressive familial intrahepatic
            cholestasis (Byler disease) to 18q21-q22, the benign recurrent
            intrahepatic cholestasis region
  JOURNAL   Hum Mol Genet 4 (6), 1049-1053 (1995)
   PUBMED   7655458
REFERENCE   7  (bases 1 to 6161)
  AUTHORS   Houwen,R.H., Baharloo,S., Blankenship,K., Raeymaekers,P., Juyn,J.,
            Sandkuijl,L.A. and Freimer,N.B.
  TITLE     Genome screening by searching for shared segments: mapping a gene
            for benign recurrent intrahepatic cholestasis
  JOURNAL   Nat Genet 8 (4), 380-386 (1994)
   PUBMED   7894490
REFERENCE   8  (bases 1 to 6161)
  AUTHORS   Amendola,M. and Squires,J.E.
  TITLE     Pediatric Genetic Cholestatic Liver Disease Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   36108118
REFERENCE   9  (bases 1 to 6161)
  AUTHORS   Bull,L.N., Morotti,R. and Squires,J.E.
  TITLE     ATP8B1 Deficiency
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301474
REFERENCE   10 (bases 1 to 6161)
  AUTHORS   Clayton,R.J., Iber,F.L., Ruebner,B.H. and McKusick,V.A.
  TITLE     Byler disease. Fatal familial intrahepatic cholestasis in an Amish
            kindred
  JOURNAL   Am J Dis Child 117 (1), 112-124 (1969)
   PUBMED   5762004
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC022724.8 and AC027097.10.
            
            On Sep 24, 2019 this sequence version replaced XM_006722481.4.
            
            Summary: This gene encodes a member of the P-type cation transport
            ATPase family, which belongs to the subfamily of
            aminophospholipid-transporting ATPases. The aminophospholipid
            translocases transport phosphatidylserine and
            phosphatidylethanolamine from one side of a bilayer to another.
            Mutations in this gene may result in progressive familial
            intrahepatic cholestasis type 1 and in benign recurrent
            intrahepatic cholestasis. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.1898280.1,
                                           SRR11853562.16480.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000648908.2/ ENSP00000497896.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-318               AC022724.8         87227-87544
            319-524             AC027097.10        11638-11843
            525-622             AC027097.10        36883-36980
            623-736             AC027097.10        38802-38915
            737-835             AC027097.10        42157-42255
            836-897             AC027097.10        42370-42431
            898-970             AC027097.10        45603-45675
            971-1041            AC027097.10        45782-45852
            1042-1124           AC027097.10        47938-48020
            1125-1283           AC027097.10        48141-48299
            1284-1372           AC027097.10        48800-48888
            1373-1563           AC027097.10        51473-51663
            1564-1772           AC027097.10        54963-55171
            1773-1816           AC027097.10        58355-58398
            1817-1973           AC027097.10        59278-59434
            1974-2162           AC027097.10        68448-68636
            2163-2275           AC027097.10        71890-72002
            2276-2440           AC027097.10        73988-74152
            2441-2552           AC027097.10        74930-75041
            2553-2628           AC027097.10        76303-76378
            2629-2761           AC027097.10        80855-80987
            2762-3050           AC027097.10        82008-82296
            3051-3274           AC027097.10        88053-88276
            3275-3358           AC027097.10        89395-89478
            3359-3604           AC027097.10        90741-90986
            3605-3743           AC027097.10        91298-91436
            3744-3874           AC027097.10        92973-93103
            3875-6161           AC027097.10        94758-97044
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.31"
     gene            1..6161
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /note="ATPase phospholipid transporting 8B1"
                     /db_xref="GeneID:5205"
                     /db_xref="HGNC:HGNC:3706"
                     /db_xref="MIM:602397"
     exon            1..318
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            319..524
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..4099
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /EC_number="7.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ATPase, class I, type 8B, member 1;
                     phospholipid-transporting ATPase IC; E1-E2 ATPase;
                     probable phospholipid-transporting ATPase IC; familial
                     intrahepatic cholestasis type 1; ATPase, aminophospholipid
                     transporter, class I, type 8B, member 1; P4-ATPase
                     flippase complex alpha subunit ATP8B1"
                     /codon_start=1
                     /product="phospholipid-transporting ATPase IC isoform 1"
                     /protein_id="NP_001361314.1"
                     /db_xref="CCDS:CCDS11965.1"
                     /db_xref="GeneID:5205"
                     /db_xref="HGNC:HGNC:3706"
                     /db_xref="MIM:602397"
                     /translation="MSTERDSETTFDEDSQPNDEVVPYSDDETEDELDDQGSAVEPEQ
                     NRVNREAEENREPFRKECTWQVKANDRKYHEQPHFMNTKFLCIKESKYANNAIKTYKY
                     NAFTFIPMNLFEQFKRAANLYFLALLILQAVPQISTLAWYTTLVPLLVVLGVTAIKDL
                     VDDVARHKMDKEINNRTCEVIKDGRFKVAKWKEIQVGDVIRLKKNDFVPADILLLSSS
                     EPNSLCYVETAELDGETNLKFKMSLEITDQYLQREDTLATFDGFIECEEPNNRLDKFT
                     GTLFWRNTSFPLDADKILLRGCVIRNTDFCHGLVIFAGADTKIMKNSGKTRFKRTKID
                     YLMNYMVYTIFVVLILLSAGLAIGHAYWEAQVGNSSWYLYDGEDDTPSYRGFLIFWGY
                     IIVLNTMVPISLYVSVEVIRLGQSHFINWDLQMYYAEKDTPAKARTTTLNEQLGQIHY
                     IFSDKTGTLTQNIMTFKKCCINGQIYGDHRDASQHNHNKIEQVDFSWNTYADGKLAFY
                     DHYLIEQIQSGKEPEVRQFFFLLAVCHTVMVDRTDGQLNYQAASPDEGALVNAARNFG
                     FAFLARTQNTITISELGTERTYNVLAILDFNSDRKRMSIIVRTPEGNIKLYCKGADTV
                     IYERLHRMNPTKQETQDALDIFANETLRTLCLCYKEIEEKEFTEWNKKFMAASVASTN
                     RDEALDKVYEEIEKDLILLGATAIEDKLQDGVPETISKLAKADIKIWVLTGDKKETAE
                     NIGFACELLTEDTTICYGEDINSLLHARMENQRNRGGVYAKFAPPVQESFFPPGGNRA
                     LIITGSWLNEILLEKKTKRNKILKLKFPRTEEERRMRTQSKRRLEAKKEQRQKNFVDL
                     ACECSAVICCRVTPKQKAMVVDLVKRYKKAITLAIGDGANDVNMIKTAHIGVGISGQE
                     GMQAVMSSDYSFAQFRYLQRLLLVHGRWSYIRMCKFLRYFFYKNFAFTLVHFWYSFFN
                     GYSAQTAYEDWFITLYNVLYTSLPVLLMGLLDQDVSDKLSLRFPGLYIVGQRDLLFNY
                     KRFFVSLLHGVLTSMILFFIPLGAYLQTVGQDGEAPSDYQSFAVTIASALVITVNFQI
                     GLDTSYWTFVNAFSIFGSIALYFGIMFDFHSAGIHVLFPSAFQFTGTASNALRQPYIW
                     LTIILAVAVCLLPVVAIRFLSMTIWPSESDKIQKHRKRLKAEEQWQRRQQVFRRGVST
                     RRSAYAFSHQRGYADLISSGRSIRKKRSPLDAIVADGTAEYRRTGDS"
     exon            525..622
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            623..736
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            737..835
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            836..897
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            898..970
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1041
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1124
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1283
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1372
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1563
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1772
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1816
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1817..1973
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2162
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2275
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2440
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2552
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2628
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2629..2761
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2762..3050
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3051..3274
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3358
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3604
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3605..3743
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3744..3874
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3875..6161
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6121..6126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /note="hexamer: AGTAAA"
     polyA_site      6140
                     /gene="ATP8B1"
                     /gene_synonym="ATPIC; BRIC; FIC1; ICP1; PFIC; PFIC1"
                     /note="major polyA site"
ORIGIN      
        1 agaggcacct ccccgagcgg agaaaccagt ttggccggtc cggcggagcg gaggcaaaag
       61 ggcggctgca gaggaagggt gcacggacgc ggggcgggga aaggcggggg agccactagc
      121 gagccgcgag agccggaggt gccgccgcca actagacacg cttccccggg cccccggcca
      181 gcccccgtgc gccatgcagc gctagcgcgg cggccagggc tggggccggg cgggcggcgg
      241 cagcggccag gtgcgcagcg agcgctccga gcggcgcgca ccaggcggat gccccagccg
      301 gggcccagcg aacgccagca ggtagttcca atttgccagc agaatgagta cagaaagaga
      361 ctcagaaacg acatttgacg aggattctca gcctaatgac gaagtggttc cctacagtga
      421 tgatgaaaca gaagatgaac ttgatgacca ggggtctgct gttgaaccag aacaaaaccg
      481 agtcaacagg gaagcagagg agaaccggga gccattcaga aaagaatgta catggcaagt
      541 caaagcaaac gatcgcaagt accacgaaca acctcacttt atgaacacaa aattcttgtg
      601 tattaaggag agtaaatatg cgaataatgc aattaaaaca tacaagtaca acgcatttac
      661 ctttatacca atgaatctgt ttgagcagtt taagagagca gccaatttat atttcctggc
      721 tcttcttatc ttacaggcag ttcctcaaat ctctaccctg gcttggtaca ccacactagt
      781 gcccctgctt gtggtgctgg gcgtcactgc aatcaaagac ctggtggacg atgtggctcg
      841 ccataaaatg gataaggaaa tcaacaatag gacgtgtgaa gtcattaagg atggcaggtt
      901 caaagttgct aagtggaaag aaattcaagt tggagacgtc attcgtctga aaaaaaatga
      961 ttttgttcca gctgacattc tcctgctgtc tagctctgag cctaacagcc tctgctatgt
     1021 ggaaacagca gaactggatg gagaaaccaa tttaaaattt aagatgtcac ttgaaatcac
     1081 agaccagtac ctccaaagag aagatacatt ggctacattt gatggtttta ttgaatgtga
     1141 agaacccaat aacagactag ataagtttac aggaacacta ttttggagaa acacaagttt
     1201 tcctttggat gctgataaaa ttttgttacg tggctgtgta attaggaaca ccgatttctg
     1261 ccacggctta gtcatttttg caggtgctga cactaaaata atgaagaata gtgggaaaac
     1321 cagatttaaa agaactaaaa ttgattactt gatgaactac atggtttaca cgatctttgt
     1381 tgttcttatt ctgctttctg ctggtcttgc catcggccat gcttattggg aagcacaggt
     1441 gggcaattcc tcttggtacc tctatgatgg agaagacgat acaccctcct accgtggatt
     1501 cctcattttc tggggctata tcattgttct caacaccatg gtacccatct ctctctatgt
     1561 cagcgtggaa gtgattcgtc ttggacagag tcacttcatc aactgggacc tgcaaatgta
     1621 ctatgctgag aaggacacac ccgcaaaagc tagaaccacc acactcaatg aacagctcgg
     1681 gcagatccat tatatcttct ctgataagac ggggacactc acacaaaata tcatgacctt
     1741 taaaaagtgc tgtatcaacg ggcagatata tggggaccat cgggatgcct ctcaacacaa
     1801 ccacaacaaa atagagcaag ttgattttag ctggaataca tatgctgatg ggaagcttgc
     1861 attttatgac cactatctta ttgagcaaat ccagtcaggg aaagagccag aagtacgaca
     1921 gttcttcttc ttgctcgcag tttgccacac agtcatggtg gataggactg atggtcagct
     1981 caactaccag gcagcctctc ccgatgaagg tgccctggta aacgctgcca ggaactttgg
     2041 ctttgccttc ctcgccagga cccagaacac catcaccatc agtgaactgg gcactgaaag
     2101 gacttacaat gttcttgcca ttttggactt caacagtgac cggaagcgaa tgtctatcat
     2161 tgtaagaacc ccagaaggca atatcaagct ttactgtaaa ggtgctgaca ctgttattta
     2221 tgaacggtta catcgaatga atcctactaa gcaagaaaca caggatgccc tggatatctt
     2281 tgcaaatgaa actcttagaa ccctatgcct ttgctacaag gaaattgaag aaaaagaatt
     2341 tacagaatgg aataaaaagt ttatggctgc cagtgtggcc tccaccaacc gggacgaagc
     2401 tctggataaa gtatatgagg agattgaaaa agacttaatt ctcctgggag ctacagctat
     2461 tgaagacaag ctacaggatg gagttccaga aaccatttca aaacttgcaa aagctgacat
     2521 taagatctgg gtgcttactg gagacaaaaa ggaaactgct gaaaatatag gatttgcttg
     2581 tgaacttctg actgaagaca ccaccatctg ctatggggag gatattaatt ctcttcttca
     2641 tgcaaggatg gaaaaccaga ggaatagagg tggcgtctac gcaaagtttg cacctcctgt
     2701 gcaggaatct ttttttccac ccggtggaaa ccgtgcctta atcatcactg gttcttggtt
     2761 gaatgaaatt cttctcgaga aaaagaccaa gagaaataag attctgaagc tgaagttccc
     2821 aagaacagaa gaagaaagac ggatgcggac ccaaagtaaa aggaggctag aagctaagaa
     2881 agagcagcgg cagaaaaact ttgtggacct ggcctgcgag tgcagcgcag tcatctgctg
     2941 ccgcgtcacc cccaagcaga aggccatggt ggtggacctg gtgaagaggt acaagaaagc
     3001 catcacgctg gccatcggag atggggccaa tgacgtgaac atgatcaaaa ctgcccacat
     3061 tggcgttgga ataagtggac aagaaggaat gcaagctgtc atgtcgagtg actattcctt
     3121 tgctcagttc cgatatctgc agaggctact gctggtgcat ggccgatggt cttacataag
     3181 gatgtgcaag ttcctacgat acttctttta caaaaacttt gcctttactt tggttcattt
     3241 ctggtactcc ttcttcaatg gctactctgc gcagactgca tacgaggatt ggttcatcac
     3301 cctctacaac gtgctgtaca ccagcctgcc cgtgctcctc atggggctgc tcgaccagga
     3361 tgtgagtgac aaactgagcc tccgattccc tgggttatac atagtgggac aaagagactt
     3421 actattcaac tataagagat tctttgtaag cttgttgcat ggggtcctaa catcgatgat
     3481 cctcttcttc atacctcttg gagcttatct gcaaaccgta gggcaggatg gagaggcacc
     3541 ttccgactac cagtcttttg ccgtcaccat tgcctctgct cttgtaataa cagtcaattt
     3601 ccagattggc ttggatactt cttattggac ttttgtgaat gctttttcaa tttttggaag
     3661 cattgcactt tattttggca tcatgtttga ctttcatagt gctggaatac atgttctctt
     3721 tccatctgca tttcaattta caggcacagc ttcaaacgct ctgagacagc catacatttg
     3781 gttaactatc atcctggctg ttgctgtgtg cttactaccc gtcgttgcca ttcgattcct
     3841 gtcaatgacc atctggccat cagaaagtga taagatccag aagcatcgca agcggttgaa
     3901 ggcggaggag cagtggcagc gacggcagca ggtgttccgc cggggcgtgt caacgcggcg
     3961 ctcggcctac gccttctcgc accagcgggg ctacgcggac ctcatctcct ccgggcgcag
     4021 catccgcaag aagcgctcgc cgcttgatgc catcgtggcg gatggcaccg cggagtacag
     4081 gcgcaccggg gacagctgat cccttacccc caggctggga tgcggccaca aagcacgtct
     4141 atttttttat gaaagactct caggactttg tgtgtgtgtg tgaattgcat tcatcacaaa
     4201 gatattgaag acaataaata atctttataa caaactcctt gggttggact attaaacaat
     4261 agcagagcca agaaatagga cttttaaaag tcctattatt cggccgggcg tggtggctca
     4321 ggtctgtaat cccagcactt taggaggctg aggaggggag atcacctgag gccaggagtt
     4381 cgagaccagc ccagccaaca tggtgaaacc ctgtctctac cataaattaa aaattagccg
     4441 ggcttggtgg tgggcacccg taatcccacc tatttgagaa gctgaagcag aagaatcgct
     4501 tgaacctggg agatggaggt tacagtgagc cgagattgcg ccactgcact ccagcctggg
     4561 tgacagagca agactctgtc ttaagaaaaa tttaaaaata ggccgggcat ggtggctcat
     4621 gcctgtcatc ctagcacttt gggaggccaa ggcaggtgga tcacttgagg tcaggagacc
     4681 agcccggcca acatggtgaa accccatctc tactaaaaat acaaaaatta gctggaaatc
     4741 gcttgaacct gggaggtgga ggttccagtg agccgagatc gtggcactgc actccaacct
     4801 gagcaacaga gtgagacacc agctcaaaaa aaatttttta ataataataa aagtcctatt
     4861 attcaactgg ttatgtacat tatggttgaa agggaacgtt ttaatccagt ctcaatccag
     4921 ggcaatagaa ttacaaagca tgttgtattt cagttcaaat ggtattgtat tataaaatta
     4981 cagttacatt ttcctttcgg tgatcttcag cataatttcc cagaggcccc tttttcctcc
     5041 ctataggcca tcttattaac agattttaaa atttatagta atgacaaatg acttatcagt
     5101 gttcatcatc tgaaagctaa gtggttcgtt caatcacttt ttcaaagttg atagtagatt
     5161 gcatggtttc atgtttcctc atattggttt attaattcta tttaatcaag gaaaataact
     5221 tcagattcca taaagtttca gtttattttt agtttactac taggtgagat agcacattac
     5281 atacttttac tatcaaatat tattttagca gcttcccata gtaccaaatg atttgattcc
     5341 ctactctcat tttttaaagc atataaatat ttatgggctt aaaaaggggg tttttaaaaa
     5401 ctgaggatat cagtaataaa ttgcagaata ttttgcaaag ctttcttttg gaaagcaaac
     5461 ttttgtgcct gcctatatgc aaagtatttt atcagggact tgaacaaaga cctcactctt
     5521 tttcacttgt cttatgtcga gagaaaaggt tattggcagc cacattccta agactgggga
     5581 atggtgtgtc cttttaaatt tgaagataac tttaggtaat tatggaaact cctcaaagag
     5641 gagaaagtaa tttttttcca gacatttttc tcattctgtg tctttcacac actagtttcc
     5701 atagttcgag aattctgttt tttaccattg ggctgtgaat gttcacaata tcagtcctgt
     5761 tgaattccta tgaggtaatc acaatgtgta tatgttcatt ttctaggtat gataaaagaa
     5821 tgtatggctt tttattctgt ggaagtaaaa tcctgaacgt ttacaacttt tccttaactt
     5881 gtaaataaaa aattgtaagt tttttctttt tttacagaaa acttagcttg tgtaattctg
     5941 ttagtttcag atttctctcc tgtttttgca aattgtggga aagattgaca atgcaaatgt
     6001 gtcaaagaca tactgttggg tgcaatatta acaattttaa atgcaaattt ctttggataa
     6061 attatttcta tattctgtaa atctgagatt taatgtatat tttgtttaaa aaaatgattt
     6121 agtaaaatct ttgaaaagta tgatcttcta aagtatttaa a
//
