LOCUS       NM_016529               9672 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens ATPase phospholipid transporting 8A2 (ATP8A2),
            transcript variant 1, mRNA.
ACCESSION   NM_016529
VERSION     NM_016529.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Matsell,E., Andersen,J.P. and Molday,R.S.
  TITLE     Functional and in silico analysis of ATP8A2 and other P4-ATPase
            variants associated with human genetic diseases
  JOURNAL   Dis Model Mech 17 (6) (2024)
   PUBMED   38436085
  REMARK    GeneRIF: Functional and in silico analysis of ATP8A2 and other
            P4-ATPase variants associated with human genetic diseases.
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Mogensen,L.S., Mikkelsen,S.A., Tadini-Buoninsegni,F., Holm,R.,
            Matsell,E., Vilsen,B., Molday,R.S. and Andersen,J.P.
  TITLE     On the track of the lipid transport pathway of the phospholipid
            flippase ATP8A2 - Mutation analysis of residues of the
            transmembrane segments M1, M2, M3 and M4
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (1), 119570 (2024)
   PUBMED   37678495
  REMARK    GeneRIF: On the track of the lipid transport pathway of the
            phospholipid flippase ATP8A2 - Mutation analysis of residues of the
            transmembrane segments M1, M2, M3 and M4.
            Review article
REFERENCE   3  (bases 1 to 9672)
  AUTHORS   Narishige,Y., Yaoita,H., Shibuya,M., Ikeda,M., Kodama,K.,
            Kawashima,A., Okubo,Y., Endo,W., Inui,T., Togashi,N., Tanaka,S.,
            Kobayashi,Y., Onuma,A., Takayama,J., Tamiya,G., Kikuchi,A., Kure,S.
            and Haginoya,K.
  TITLE     Two Siblings with Cerebellar Ataxia, Mental Retardation, and
            Disequilibrium Syndrome 4 and a Novel Variant of ATP8A2
  JOURNAL   Tohoku J Exp Med 256 (4), 321-326 (2022)
   PUBMED   35321980
  REMARK    GeneRIF: Two Siblings with Cerebellar Ataxia, Mental Retardation,
            and Disequilibrium Syndrome 4 and a Novel Variant of ATP8A2.
REFERENCE   4  (bases 1 to 9672)
  AUTHORS   van der Mark,V.A., Elferink,R.P. and Paulusma,C.C.
  TITLE     P4 ATPases: flippases in health and disease
  JOURNAL   Int J Mol Sci 14 (4), 7897-7922 (2013)
   PUBMED   23579954
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9672)
  AUTHORS   Coleman,J.A. and Molday,R.S.
  TITLE     Critical role of the beta-subunit CDC50A in the stable expression,
            assembly, subcellular localization, and lipid transport activity of
            the P4-ATPase ATP8A2
  JOURNAL   J Biol Chem 286 (19), 17205-17216 (2011)
   PUBMED   21454556
  REMARK    GeneRIF: CDC50A is the beta-subunit of ATP8A2 and is crucial for
            the correct folding, stable expression, export from endoplasmic
            reticulum, and phosphatidylserine flippase activity of ATP8A2
REFERENCE   6  (bases 1 to 9672)
  AUTHORS   van der Velden,L.M., Wichers,C.G., van Breevoort,A.E.,
            Coleman,J.A., Molday,R.S., Berger,R., Klomp,L.W. and van de
            Graaf,S.F.
  TITLE     Heteromeric interactions required for abundance and subcellular
            localization of human CDC50 proteins and class 1 P4-ATPases
  JOURNAL   J Biol Chem 285 (51), 40088-40096 (2010)
   PUBMED   20947505
REFERENCE   7  (bases 1 to 9672)
  AUTHORS   Cacciagli,P., Haddad,M.R., Mignon-Ravix,C., El-Waly,B., Moncla,A.,
            Missirian,C., Chabrol,B. and Villard,L.
  TITLE     Disruption of the ATP8A2 gene in a patient with a t(10;13) de novo
            balanced translocation and a severe neurological phenotype
  JOURNAL   Eur J Hum Genet 18 (12), 1360-1363 (2010)
   PUBMED   20683487
REFERENCE   8  (bases 1 to 9672)
  AUTHORS   Coleman,J.A., Kwok,M.C. and Molday,R.S.
  TITLE     Localization, purification, and functional reconstitution of the
            P4-ATPase Atp8a2, a phosphatidylserine flippase in photoreceptor
            disc membranes
  JOURNAL   J Biol Chem 284 (47), 32670-32679 (2009)
   PUBMED   19778899
  REMARK    GeneRIF: P4-ATPase Atp8a2 is a phosphatidylserine flippase in
            photoreceptor disc membranes
REFERENCE   9  (bases 1 to 9672)
  AUTHORS   Halleck,M.S., Lawler,J.F. Jr., Blackshaw,S., Gao,L., Nagarajan,P.,
            Hacker,C., Pyle,S., Newman,J.T., Nakanishi,Y., Ando,H.,
            Weinstock,D., Williamson,P. and Schlegel,R.A.
  TITLE     Differential expression of putative transbilayer amphipath
            transporters
  JOURNAL   Physiol Genomics 1 (3), 139-150 (1999)
   PUBMED   11015572
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 9672)
  AUTHORS   Sun,X.L., Li,D., Fang,J., Noyes,I., Casto,B., Theil,K., Shuler,C.
            and Milo,G.E.
  TITLE     Changes in levels of normal ML-1 gene transcripts associated with
            the conversion of human nontumorigenic to tumorigenic phenotypes
  JOURNAL   Gene Expr 8 (2), 129-139 (1999)
   PUBMED   10551800
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL138958.18, DA326706.1,
            AK127263.1, AF236871.2, AL669971.2, AK094653.1, AL136438.10,
            AK126031.1, AL390129.1, AL138815.6, AL139004.11 and BP396213.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_016529.5.
            
            Summary: The protein encoded by this gene is a member of the P4
            ATPase family of proteins, which are thought to be involved in a
            process called lipid flipping, whereby phospholipids are
            translocated inwards from the exoplasmic leaflet to the cytosolic
            leaflet of the cell membrane, which aids in generating and
            maintaining asymmetry in membrane lipids. This protein is predicted
            to contain an E1 E2 ATPase, a haloacid dehalogenase-like hydrolase
            (HAD) domain, and multiple transmembrane domains. Associations
            between this protein and cell cycle control protein 50A are
            important for translocation of phosphatidylserine across membranes.
            Mutations in this gene have been associated with a syndrome
            (CAMRQ4) characterized by cerebellar ataxia and cognitive
            disabilities. In addition, a translocation breakpoint within this
            gene was observed in an individual with neurological dysfunction.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jul 2017].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.42602.1,
                                           SRR1803613.76798.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381655.7/ ENSP00000371070.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AL138958.18        34664-34700
            38-97               DA326706.1         1-60
            98-382              AK127263.1         1-285
            383-1901            AF236871.2         24-1542
            1902-1921           AK127263.1         1805-1824
            1922-1922           AL669971.2         2544-2544
            1923-1956           AK127263.1         1826-1859
            1957-2524           AK094653.1         645-1212
            2525-2525           AL136438.10        129434-129434       c
            2526-2542           AK127263.1         2429-2445
            2543-4115           AK126031.1         1719-3291
            4116-4679           AK127263.1         4019-4582
            4680-5103           AL390129.1         1540-1963
            5104-6144           AL138815.6         1-1041              c
            6145-9408           AL139004.11        101-3364
            9409-9672           BP396213.1         264-527
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.13"
     gene            1..9672
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /note="ATPase phospholipid transporting 8A2"
                     /db_xref="GeneID:51761"
                     /db_xref="HGNC:HGNC:13533"
                     /db_xref="MIM:605870"
     exon            1..315
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             240..3806
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /EC_number="7.6.2.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ATPase, aminophospholipid transporter-like, class I, type
                     8A, member 2; probable phospholipid-transporting ATPase
                     IB; P4-ATPase flippase complex alpha subunit ATP8A2;
                     ATPase, aminophospholipid transporter, class I, type 8A,
                     member 2"
                     /codon_start=1
                     /product="phospholipid-transporting ATPase IB isoform 1"
                     /protein_id="NP_057613.4"
                     /db_xref="CCDS:CCDS41873.1"
                     /db_xref="GeneID:51761"
                     /db_xref="HGNC:HGNC:13533"
                     /db_xref="MIM:605870"
                     /translation="MLNGAGLDKALKMSLPRRSRIRSSVGPVRSSLGYKKAEDEMSRA
                     TSVGDQLEAPARTIYLNQPHLNKFRDNQISTAKYSVLTFLPRFLYEQIRRAANAFFLF
                     IALLQQIPDVSPTGRYTTLVPLIIILTIAGIKEIVEDFKRHKADNAVNKKKTIVLRNG
                     MWHTIMWKEVAVGDIVKVVNGQYLPADVVLLSSSEPQAMCYVETANLDGETNLKIRQG
                     LSHTADMQTREVLMKLSGTIECEGPNRHLYDFTGNLNLDGKSLVALGPDQILLRGTQL
                     RNTQWVFGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSAG
                     ALYWNRSHGEKNWYIKKMDTTSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQALF
                     INWDTDMYYIGNDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNIMNFKKCSIAGVTY
                     GHFPELAREPSSDDFCRMPPPCSDSCDFDDPRLLKNIEDRHPTAPCIQEFLTLLAVCH
                     TVVPEKDGDNIIYQASSPDEAALVKGAKKLGFVFTARTPFSVIIEAMGQEQTFGILNV
                     LEFSSDRKRMSVIVRTPSGRLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGL
                     RTLCVAYADLSENEYEEWLKVYQEASTILKDRAQRLEECYEIIEKNLLLLGATAIEDR
                     LQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATR
                     AAITQHCTDLGNLLGKENDVALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSP
                     LQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQ
                     FSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIG
                     LYNVIFTALPPFTLGIFERSCTQESMLRFPQLYKITQNGEGFNTKVFWGHCINALVHS
                     LILFWFPMKALEHDTVLTSGHATDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLA
                     VWGSMLTWLVFFGIYSTIWPTIPIAPDMRGQATMVLSSAHFWLGLFLVPTACLIEDVA
                     WRAAKHTCKKTLLEEVQELETKSRVLGKAVLRDSNGKRLNERDRLIKRLGRKTPPTLF
                     RGSSLQQGVPHGYAFSQEEHGAVSQEEVIRAYDTTKKKSRKK"
     exon            316..460
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            461..560
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            561..659
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            660..705
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..746
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            747..820
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            821..890
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1018
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1130
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1296
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1424
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1502
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1591
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1636
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1712
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1713..1818
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1901
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..1951
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2021
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2106
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2107..2246
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2385
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2386..2450
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2623
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2624..2807
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2808..2918
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2919..2993
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2994..3116
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3117..3195
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3196..3257
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3258..3314
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3315..3422
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3423..3511
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3512..3616
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3617..3708
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     exon            3709..9672
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9651..9656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /note="hexamer: AATAAA"
     polyA_site      9672
                     /gene="ATP8A2"
                     /gene_synonym="ATP; ATPIB; CAMRQ4; IB; ML-1"
                     /note="major polyA site"
ORIGIN      
        1 gcagcccggg ccagcggggc ccgccgcggt ggcgttcgcg gatgctgccg cattggccgc
       61 tgcggcacgg acggcgcagc ctcgggcgcg gcccggcaca ggcgccggcg gtccccgcca
      121 gctagcagcc cggcgaggcg ctggcccacc catggtcctc gggcggcggc ccctgcgccc
      181 agccctgcgc gtagcctccg tctctcgccc ggggccgccg agcccccgac acgggcgaga
      241 tgctgaacgg cgcaggcctg gacaaagctc ttaagatgtc cctgccgcgg aggtcgagga
      301 tccgctcgtc cgtgggacct gttcgttctt ctttgggcta taagaaggca gaggatgaga
      361 tgtcccgggc cacgtctgtt ggagaccagc tggaggcacc cgcccgcacc atttacctca
      421 accaaccgca tctcaacaaa ttccgcgaca accagatcag tacggccaag tacagcgtgt
      481 tgacatttct acctcgattc ttgtatgagc agattagaag agctgctaat gccttctttc
      541 tcttcattgc cttattacag caaattccag atgtatctcc aacaggaaga tataccaccc
      601 tggtgccatt gatcattatt ttaacaattg caggcatcaa agagattgta gaagatttta
      661 agcgacacaa ggcagacaat gcagttaaca aaaagaaaac aatagtgtta agaaatggta
      721 tgtggcatac cattatgtgg aaagaggtgg cagtgggaga cattgtgaag gtcgtcaatg
      781 ggcagtatct tccagcagat gtggtcctgc tgtcatccag tgaacctcag gcaatgtgtt
      841 atgttgaaac agctaatctg gatggggaga cgaaccttaa aatacgtcag ggtttgagtc
      901 acactgctga catgcaaaca cgtgaagttc tgatgaagtt atctggaact atagagtgtg
      961 aagggcccaa ccgccacctc tatgacttca ctggaaactt gaacttagat gggaaaagcc
     1021 ttgttgccct tgggcctgac cagatcttat taagaggtac acagcttaga aatactcagt
     1081 gggtctttgg catagttgtt tatactggac acgacaccaa actcatgcag aattcaacca
     1141 aagcgcctct caagagatca aatgttgaga aggtgactaa cgtgcagatc ctggtgttgt
     1201 ttggcatcct cttggtcatg gccttggtga gctcggcggg ggccctgtac tggaacaggt
     1261 ctcatggtga aaagaactgg tacatcaaga agatggacac cacctcagat aattttggat
     1321 acaacctact gacgttcatc atcttataca acaatcttat tcccatcagt ctgttggtga
     1381 ctcttgaggt tgtgaagtat actcaagccc ttttcataaa ctgggacaca gatatgtatt
     1441 atataggaaa tgacactcct gccatggcca ggacatcaaa ccttaatgaa gagcttgggc
     1501 aggtgaaata tctcttttct gacaagactg gaacgcttac atgcaatatc atgaacttta
     1561 agaagtgcag cattgccgga gtaacctatg gtcacttccc agaattggca agagagccgt
     1621 cttcagatga cttctgtcgg atgcctcctc cctgtagtga ttcctgtgac tttgatgacc
     1681 ccaggctgtt gaagaacatt gaggatcgcc atcccacagc cccttgcatt caggagttcc
     1741 tcacccttct ggccgtgtgc cacacggttg ttcctgagaa ggatggagat aacatcatct
     1801 accaggcctc ttccccagat gaagctgctt tggtgaaagg agctaaaaag ctgggctttg
     1861 tcttcacagc cagaacacca ttctcagtca tcatagaagc gatgggacag gaacaaacat
     1921 tcggaatcct taatgtcctg gaattttcta gtgacagaaa aagaatgtct gtaattgttc
     1981 gaactccttc aggacgactt cggctttact gtaaaggggc tgataatgtg atttttgaga
     2041 gactttcaaa agactcaaaa tatatggagg aaacattatg ccatctggaa tactttgcca
     2101 cggaaggctt gcggactctc tgtgtggctt atgctgatct ctctgagaat gagtatgagg
     2161 agtggctgaa agtctatcag gaagccagca ccatattgaa ggacagagct caacggttgg
     2221 aagagtgtta cgagatcatt gagaagaatt tgctgctact tggagccaca gccatagaag
     2281 atcgccttca agcaggagtt ccagaaacca tcgcaacact gttgaaggca gaaattaaaa
     2341 tatgggtgtt gacaggagac aaacaagaaa ctgcgattaa tatagggtat tcctgccgat
     2401 tggtatcgca gaatatggcc cttatcctat tgaaggagga ctctttggat gccacaaggg
     2461 cagccattac tcagcactgc actgaccttg ggaatttgct gggcaaggaa aatgacgtgg
     2521 ccctgatcat cgatggccac accctgaagt acgcgctctc cttcgaagtc cggaggagtt
     2581 tcctggattt ggcactctcg tgcaaagcgg tcatatgctg cagagtgtct cctctgcaga
     2641 agtctgagat agtggatgtg gtgaagaagc gggtgaaggc catcaccctc gccatcggag
     2701 acggcgccaa cgatgtcggg atgatccaga cagcccacgt gggtgtggga atcagtggga
     2761 atgaaggcat gcaggccacc aacaactcgg attacgccat cgcacagttt tcctacttag
     2821 agaagcttct gttggttcat ggagcctgga gctacaaccg ggtgaccaag tgcatcttgt
     2881 actgcttcta taagaacgtg gtcctgtata ttattgagct ttggttcgcc tttgttaatg
     2941 gattttctgg gcagatttta tttgaacgtt ggtgcatcgg cctgtacaat gtgattttca
     3001 ccgctttgcc gcccttcact ctgggaatct ttgagaggtc ttgcactcag gagagcatgc
     3061 tcaggtttcc ccagctctac aaaatcaccc agaatggcga aggcttcaac acaaaggttt
     3121 tctggggtca ctgcatcaac gccttggtcc actccctcat cctcttctgg tttcccatga
     3181 aagctctgga gcatgatact gtgttgacaa gtggtcatgc taccgactat ttatttgttg
     3241 gaaatattgt ttacacatat gttgttgtta ctgtttgtct gaaagctggt ttggagacca
     3301 cagcttggac taaattcagt catctggctg tctggggaag catgctgacc tggctggtgt
     3361 tttttggcat ctactcgacc atctggccca ccattcccat tgctccagat atgagaggac
     3421 aggcaactat ggtcctgagc tccgcacact tctggttggg attatttctg gttcctactg
     3481 cctgtttgat tgaagatgtg gcatggagag cagccaagca cacctgcaaa aagacattgc
     3541 tggaggaggt gcaggagctg gaaaccaagt ctcgagtcct gggaaaagcg gtgctgcggg
     3601 atagcaatgg aaagaggctg aacgagcgcg accgcctgat caagaggctg ggccggaaga
     3661 cgcccccgac gctgttccgg ggcagctccc tgcagcaggg cgtcccgcat gggtatgctt
     3721 tttctcaaga agaacacgga gctgttagtc aggaagaagt catccgtgct tatgacacca
     3781 ccaaaaagaa atccaggaag aaataagaca tgaattttcc tgactgatct taggaaagag
     3841 attcagtttg ttgcacccag tgttaacaca tctttgtcag agaagactgg cgtcagcagc
     3901 caaaacacca ggaaacacat ttctgtggcc ttagccaagc agtttgttag ttacatattc
     3961 cctcgcaaac ctggagtgca gaccacaggg gaagctatct ttgccctccc aactcgtctg
     4021 cagtgcttag cctaactttt gtttatgtcg ttatgaagca ttcaactgtg ctctgtgagg
     4081 tgtgaaatta aaaacattat gtttcaccaa tatttaaaca tcagtactag ttgtcctggg
     4141 agaaagggaa aggagtttta tgttgcgtga gaggcccatc ctgtgtaatt ggagcagggc
     4201 acacttgctt cctgttgagt taactcagag gttaagtcca acgggccaca tgcagacttc
     4261 actgtaggca ggttgctctc ctgctttgat tcctgttttg tgtgtaaaat tggcataaac
     4321 ttcttgattg cagtgaaatc acaaaattct ctatcggggt ggtcaacctg agaacattta
     4381 tttgaacctc ttagccacat ttccagcagg gcaaaccaac tgatgccctg gaagagtctt
     4441 ccgcaccctc tccaggtgca ctcggcccag tcctgccgcc gtgtccaggc gcccctcacc
     4501 cccacacctg ctgcatggct ggccacacca ctcagtgcat ggcggccgat gggcagccaa
     4561 cccaaacccg cgcctttcct tgttccactg cagactcaga tacagatgcg aaaaattcct
     4621 tcttccaccg cccttctcgt tctgtaaaga aagaaaagaa acatagcctt tctgcatata
     4681 ttctaaacgt ctctctgcct ctgtctgaca tggggccacc ccacaggtca gagtggtggt
     4741 agaacccctt caggactccc agccgtggtc aggctctgaa tactcccttc ccaacatcca
     4801 gactgctggg cctttggcat ccacttacat tagaacccac gtttgtttca gagcacattt
     4861 tggactttca ctgttgggaa atgaatgaat ttataacatg cctgcacagc gaaggaacac
     4921 acctgtcgct cttagctcta gagtcagagg atgagtaaac ccagatgcaa gagtatagga
     4981 cattgagtgg ggagaacaag acgaccacag aagtcctcag aaggagaagg aaggacacgg
     5041 agacactgag aggaggacac agaggaatcg ccaccagatc tttgcagtag aaactctgaa
     5101 ataggccttg agacccagac aattgtcatg atcagcccag tgtgtatgag agcttaaaca
     5161 agaccttcac acacagttct aattgaaaag aattccaaac atttcaggga aacacctttt
     5221 taaaaaatat actgacatgg tttcctttct gtatcccatc agtgttagac acaggtacct
     5281 atcagaatac ggcatatcct gaattatcta agacaaggaa gtgaagagcg gaatcactga
     5341 ctttcccaac tgcagagtcc cacacttaaa tctcaacaga acctagatgt cttcaggcat
     5401 gaatgtgagc acctggagcc cttactaaac aacctgcctg gagccctgtt ctctgctctg
     5461 gttgccaaac cagatttttg gatggcctaa atacttgtgg gcaactgtta ctggtgaaaa
     5521 atgggagtgg agagcatgtt ttggattttc actgtgggga aatgaatgaa tttatcacat
     5581 gactgtgcag cgaaggagca cacctgtcac tcttagctct agagtcggag gatgagtcaa
     5641 ccctgtagga atgattgtgc taaattagga aataacactt tagtgcactt tgcttcctaa
     5701 cccttttttg tcagaaatct acaacctact gggcatggaa atgatttaaa atatgatctg
     5761 cattgactaa gaaaactcat cctcacttat gaggtgacca ggatgcagag agggcctgag
     5821 gggcagcaga acaatgcaat tcacaggtga ggctccaaaa agggccattt ctttctcccc
     5881 aaggtgcata gaacattttt aaatatcaga gtacccagca agacggaagg actgtcatgc
     5941 aggaactcta acacaaaatg tgcctatgac taacacgaca aaccaaggtg tatggtttct
     6001 ttgcacaccc agaaaggtga tggccaagat acagaggaga aacgaaaact ggaattgaat
     6061 gtgcattagg catcttaact attcatattt tgtctcattt gggaactaag cctatttgga
     6121 aagagtggat tagaaattga attctttgtc aaacagttag ctctgtcttg aacttgaagc
     6181 cttccaaatc agaattatgt atttaagtgt gtgtttgtgt ttgtttgggg tttttgtttc
     6241 ttacattaga gttcatattt tctgggattt aaggatacag gtgtattttc ctattctctt
     6301 gagaagtaga ctaaacaata tttgcagtga tgacagacgc acacagaaga aaacattaga
     6361 agagatgcct ttctatcctc tcatgtggtt gagcattctt acagccaaat gactaaattg
     6421 gctgtaaatt ggtttctagg aggagcagct gtcactacgt gtgatgtata atatttagcc
     6481 agggccaact caggggctgt gtcatttgtc ctatctttaa attgaggtca gagtgtcaca
     6541 cacaacccct cccctggggc agggtggact ggactaggtt gcatacgctg gctgtcagct
     6601 cagcaaaaat gccacatggg gcttgtcact ccgccagaca ctcactgact cacgggctgg
     6661 caggtcacgc tcggttagcg ctccagggcc tgactctgga ttgagctaaa ctggcagaat
     6721 ccactcaggc caacccttgg tctactgctg tgtacagaca gcccagtcct gtgttgagtc
     6781 tctcttctcc tggatgaccc tgcaaaagga agagaccagt tgtactgtgg gcttagttct
     6841 gggtttccac tgcacactga cacgtgccag caagggtagc tgtggaaaac acgtatcagg
     6901 agagcagagt agtctggatg aatactctgt gctgatcaga acgggctcgg tcataattta
     6961 ggagtggagc tgatgtgtct gtcctgcctc agtgcagaat attccaggct tcttgaccag
     7021 ggttatgttc acccatctcc caacacacac atgcacacat gaaaacaaaa acatggtaac
     7081 tcttttggtt cggcacacca tacaaaacgt gctttggggc gaggagtgct gtttctttta
     7141 acacttgtag aggaatatcc tgagaagtga acctgggctt tgggagagct cagctgagaa
     7201 ttctgttgac attttctttc ccttgacaag gcttcaggtt cgtctcacta tagggagctg
     7261 gctgtgaaaa tcagtaataa cttgtcaaga agcagtgaaa aatgcggggg ggtggaaaga
     7321 gcctaggtaa ctaatgtctt ataatgcata tgtatgtaaa tattacagga tttaaggttg
     7381 aattttttaa aaagaaagtt atagtctgta atttccattt gttataataa tgacctttaa
     7441 tcttgtcatt tggaaccata aagcattttt atcaggtacc tctgttccaa gggatttatg
     7501 tcttagacca tagctgaatt gaatgtttgc aaaacactgc tataggataa ggtggtcttt
     7561 agttttgaac gtgtgaaagg actgcacact tttcagccag ggtttgagtt actgcccagg
     7621 gtcatcgtct caaagtaatt caaggagtga tttaacatca gcatttgaaa tgtagtcttc
     7681 atctcctggg atccataaaa aaatgtgaac agggaaatgg tggctaagca gagcctgaaa
     7741 taataacttg gcaaagaaat gagtttatca ggtcgagtca aaacatggca tcccctgtta
     7801 cactcaagaa atgttttctt catgtaaatg tttatacggg catatataat cacaatggga
     7861 acagttaaaa ccccctccct tcaaaaaaag aaaatctata tcagttgggt ttggttttgg
     7921 ttcttcattg gctcaaggca gttaactgtc tcagtatagc cttcggggag atttaacctc
     7981 attctagcca tttttccatc ctgaaggcca agaaggacta ttagaagggt ttttgagggg
     8041 tttcggaggt gagggcccaa gacccccata atgacatcat taggcattct tgaaaggtgt
     8101 taacagacca gcacgctcga tgtgttgtac ccttcattta tttttttttc tcagcatccc
     8161 aagttgtcca tcagtacatt ccccttcacc ttacctgatc ttcatttaat gcccagtctg
     8221 taactaatgt tctgtttaaa gctctcttaa tttgttggct atgagtgatt tgtgaatctg
     8281 ggatgtaacc ctgagtgagg agaaaagggc attgagccag cataaatctg aatgggcaga
     8341 agccacaaac aaatgggagc agaaaagaag ggcgagctcc cccgcgccgc accccgtgta
     8401 taatccagtg ttaacctctg aggaacacct cggctctccc aactgaggag agacaaaaga
     8461 gggattcttt ttacacccag gctggcgcat tttcaagtga caatctcggg ctctagttgc
     8521 ctttgagata tccactctgc tctttctccc aggcctcaga ccaagaaaaa catgctctca
     8581 agattagccc ataggcagtt cttgtgacct ggctgaagaa agaaaggaga cctgtttgtt
     8641 ttaaaagtcg ggcgcaaagt gtcaggtggc tttgatttat gacagaaaga ggaagaagaa
     8701 agttggagta atagcactat ccaaataacc ctgcccagga aacttggggg tcaagagagc
     8761 ttatcaagag ccttttatag gtaagctctt ccgtgtgaaa gagaaaaggc cggaaagagg
     8821 gagagagccc agagagcagg ctgcgaactc acttccaatg ggattggagt ctgttgtatt
     8881 cttaagagat agcctgatat gtggtttata ggtgaatcaa taaacaccaa caacaacaaa
     8941 aaaaaaaaaa aaaaaaaagg aagaaaagaa aaaaaggaaa ccagccctgt catggaattt
     9001 ctctccttcc ctgcacagta aagacttttg ggttttcatg gataaaatca atgtcagtac
     9061 tgaaactcct actctcccct cccgccccac tctcccccgt tgcccgagat ggccaagttc
     9121 aggcctgtgc aatgccgctt ccctctgagc ctccctctca agggccacgc aggcagctgc
     9181 agcagggcca gctgcaggat ggggctgccg gtcactgaat tgtcgttcaa atgcatcatc
     9241 tttgtggcgt ctttctcatg cgagcaaagc cacgtgctct cctgtctgct gtcacatctg
     9301 tgcctggatt gcttaaatat tgtttgtgat ggggaggttt taatctggtg atgcagaggg
     9361 aagcagggct gtgggggcac gtttaattgg ctcccagcag cgtggggggt gcttctatgg
     9421 tgtgtggggt tttttgttgc ctccctctag aagtgttacc gttttcacgt cctattaatg
     9481 tcctctggtt gttaaattac agcagcacat tacagtgcac tgggttccct cctggagtga
     9541 atacaaacgg agggcatcta cttgtatttt tagaagtttt gggagaattt agtgatttgt
     9601 ggctttgatc aatcctgttg actggtgtat gtctgcgcaa acctgtttca aataaatctt
     9661 ttgttaaagt aa
//
