LOCUS       NM_006095               8270 bp    mRNA    linear   PRI 17-MAR-2024
DEFINITION  Homo sapiens ATPase phospholipid transporting 8A1 (ATP8A1),
            transcript variant 1, mRNA.
ACCESSION   NM_006095
VERSION     NM_006095.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Deng,C., Huo,M., Chu,H., Zhuang,X., Deng,G., Li,W., Wei,H.,
            Zeng,L., He,Y., Liu,H., Li,J., Zhang,C. and Chen,H.
  TITLE     Exosome circATP8A1 induces macrophage M2 polarization by regulating
            the miR-1-3p/STAT6 axis to promote gastric cancer progression
  JOURNAL   Mol Cancer 23 (1), 49 (2024)
   PUBMED   38459596
  REMARK    GeneRIF: Exosome circATP8A1 induces macrophage M2 polarization by
            regulating the miR-1-3p/STAT6 axis to promote gastric cancer
            progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Kook,S., Wang,P., Meng,S., Jetter,C.S., Sucre,J.M.S.,
            Benjamin,J.T., Gokey,J.J., Hanby,H.A., Jaume,A., Goetzl,L.,
            Marks,M.S. and Guttentag,S.H.
  TITLE     AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies
            suppresses activation of YAP in alveolar epithelial type 2 cells
  JOURNAL   Proc Natl Acad Sci U S A 118 (20) (2021)
   PUBMED   33990468
  REMARK    GeneRIF: AP-3-dependent targeting of flippase ATP8A1 to lamellar
            bodies suppresses activation of YAP in alveolar epithelial type 2
            cells.
REFERENCE   3  (bases 1 to 8270)
  AUTHORS   Hiraizumi,M., Yamashita,K., Nishizawa,T. and Nureki,O.
  TITLE     Cryo-EM structures capture the transport cycle of the P4-ATPase
            flippase
  JOURNAL   Science 365 (6458), 1149-1155 (2019)
   PUBMED   31416931
  REMARK    GeneRIF: cryo-electron microscopy structure of six intermediates of
            the human flippase ATP8A1 bound to the partner protein CDC50a; ATP
            binding and autophosphorylation of ATP8A1 drive a cycle of
            conformations in which lipids bind differently, powering
            translocation.
REFERENCE   4  (bases 1 to 8270)
  AUTHORS   Jing,W., Yabas,M., Broer,A., Coupland,L., Gardiner,E.E., Enders,A.
            and Broer,S.
  TITLE     Calpain cleaves phospholipid flippase ATP8A1 during apoptosis in
            platelets
  JOURNAL   Blood Adv 3 (3), 219-229 (2019)
   PUBMED   30674456
  REMARK    GeneRIF: ATP8A1 is cleaved by the cysteine protease calpain during
            apoptosis, and the cleavage is prevented indirectly by caspase
            inhibition, involving blockage of calcium influx into platelets and
            subsequent calpain activation.
REFERENCE   5  (bases 1 to 8270)
  AUTHORS   Li,Q., Yang,Y. and Liu,Y.
  TITLE     Over-Expression of ATPase II Alleviates Ethanol-Induced Hepatocyte
            Injury in HL-7702 Cells
  JOURNAL   Med Sci Monit 24, 8372-8382 (2018)
   PUBMED   30457983
  REMARK    GeneRIF: Over-expression of ATP8A1 alleviated ethanol-induced
            hepatocyte injury. Moreover, the PI3K/Akt signaling pathway appears
            to participate in inhibition of ethanol-induced hepatocyte
            apoptosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8270)
  AUTHORS   Paulusma,C.C. and Oude Elferink,R.P.
  TITLE     The type 4 subfamily of P-type ATPases, putative aminophospholipid
            translocases with a role in human disease
  JOURNAL   Biochim Biophys Acta 1741 (1-2), 11-24 (2005)
   PUBMED   15919184
  REMARK    Review article
REFERENCE   7  (bases 1 to 8270)
  AUTHORS   Kuhlbrandt,W.
  TITLE     Biology, structure and mechanism of P-type ATPases
  JOURNAL   Nat Rev Mol Cell Biol 5 (4), 282-295 (2004)
   PUBMED   15071553
  REMARK    Review article
REFERENCE   8  (bases 1 to 8270)
  AUTHORS   Halleck,M.S., Lawler,J.F. Jr., Blackshaw,S., Gao,L., Nagarajan,P.,
            Hacker,C., Pyle,S., Newman,J.T., Nakanishi,Y., Ando,H.,
            Weinstock,D., Williamson,P. and Schlegel,R.A.
  TITLE     Differential expression of putative transbilayer amphipath
            transporters
  JOURNAL   Physiol Genomics 1 (3), 139-150 (1999)
   PUBMED   11015572
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 8270)
  AUTHORS   Mouro,I., Halleck,M.S., Schlegel,R.A., Mattei,M.G., Williamson,P.,
            Zachowski,A., Devaux,P., Cartron,J.P. and Colin,Y.
  TITLE     Cloning, expression, and chromosomal mapping of a human ATPase II
            gene, member of the third subfamily of P-type ATPases and
            orthologous to the presumed bovine and murine aminophospholipid
            translocase
  JOURNAL   Biochem Biophys Res Commun 257 (2), 333-339 (1999)
   PUBMED   10198212
REFERENCE   10 (bases 1 to 8270)
  AUTHORS   Halleck,M.S., Pradhan,D., Blackman,C., Berkes,C., Williamson,P. and
            Schlegel,R.A.
  TITLE     Multiple members of a third subfamily of P-type ATPases identified
            by genomic sequences and ESTs
  JOURNAL   Genome Res 8 (4), 354-361 (1998)
   PUBMED   9548971
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BP232466.1, AF067820.1 and
            AC084010.6.
            
            On Sep 25, 2007 this sequence version replaced NM_006095.1.
            
            Summary: The P-type adenosinetriphosphatases (P-type ATPases) are a
            family of proteins which use the free energy of ATP hydrolysis to
            drive uphill transport of ions across membranes. Several
            subfamilies of P-type ATPases have been identified. One subfamily
            catalyzes transport of heavy metal ions. Another subfamily
            transports non-heavy metal ions (NMHI). The protein encoded by this
            gene is a member of the third subfamily of P-type ATPases and acts
            to transport amphipaths, such as phosphatidylserine. Two transcript
            variants encoding different isoforms have been found for this gene.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.15176.1,
                                           SRR1803615.241366.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381668.9/ ENSP00000371084.5
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-556               BP232466.1         1-556
            557-3724            AF067820.1         325-3492
            3725-8270           AC084010.6         6419-10964          c
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p13"
     gene            1..8270
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /note="ATPase phospholipid transporting 8A1"
                     /db_xref="GeneID:10396"
                     /db_xref="HGNC:HGNC:13531"
                     /db_xref="MIM:609542"
     exon            1..281
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             233..3727
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /EC_number="7.6.2.1"
                     /note="isoform a is encoded by transcript variant 1;
                     ATPase II; aminophospholipid translocase; probable
                     phospholipid-transporting ATPase IA; chromaffin granule
                     ATPase II; ATPase class I type 8A member 1; P4-ATPase
                     flippase complex alpha subunit ATP8A1; ATPase,
                     aminophospholipid transporter (APLT), class I, type 8A,
                     member 1"
                     /codon_start=1
                     /product="phospholipid-transporting ATPase IA isoform a"
                     /protein_id="NP_006086.1"
                     /db_xref="CCDS:CCDS3466.1"
                     /db_xref="GeneID:10396"
                     /db_xref="HGNC:HGNC:13531"
                     /db_xref="MIM:609542"
                     /translation="MPTMRRTVSEIRSRAEGYEKTDDVSEKTSLADQEEVRTIFINQP
                     QLTKFCNNHVSTAKYNIITFLPRFLYSQFRRAANSFFLFIALLQQIPDVSPTGRYTTL
                     VPLLFILAVAAIKEIIEDIKRHKADNAVNKKQTQVLRNGAWEIVHWEKVAVGEIVKVT
                     NGEHLPADLISLSSSEPQAMCYIETSNLDGETNLKIRQGLPATSDIKDVDSLMRISGR
                     IECESPNRHLYDFVGNIRLDGHGTVPLGADQILLRGAQLRNTQWVHGIVVYTGHDTKL
                     MQNSTSPPLKLSNVERITNVQILILFCILIAMSLVCSVGSAIWNRRHSGKDWYLNLNY
                     GGASNFGLNFLTFIILFNNLIPISLLVTLEVVKFTQAYFINWDLDMHYEPTDTAAMAR
                     TSNLNEELGQVKYIFSDKTGTLTCNVMQFKKCTIAGVAYGHVPEPEDYGCSPDEWQNS
                     QFGDEKTFSDSSLLENLQNNHPTAPIICEFLTMMAVCHTAVPEREGDKIIYQAASPDE
                     GALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLNVLEFTSARKRMSVIVRTPSGK
                     LRLYCKGADTVIYDRLAETSKYKEITLKHLEQFATEGLRTLCFAVAEISESDFQEWRA
                     VYQRASTSVQNRLLKLEESYELIEKNLQLLGATAIEDKLQDQVPETIETLMKADIKIW
                     ILTGDKQETAINIGHSCKLLKKNMGMIVINEGSLDGTRETLSRHCTTLGDALRKENDF
                     ALIIDGKTLKYALTFGVRQYFLDLALSCKAVICCRVSPLQKSEVVEMVKKQVKVVTLA
                     IGDGANDVSMIQTAHVGVGISGNEGLQAANSSDYSIAQFKYLKNLLMIHGAWNYNRVS
                     KCILYCFYKNIVLYIIEIWFAFVNGFSGQILFERWCIGLYNVMFTAMPPLTLGIFERS
                     CRKENMLKYPELYKTSQNALDFNTKVFWVHCLNGLFHSVILFWFPLKALQYGTAFGNG
                     KTSDYLLLGNFVYTFVVITVCLKAGLETSYWTWFSHIAIWGSIALWVVFFGIYSSLWP
                     AIPMAPDMSGEAAMLFSSGVFWMGLLFIPVASLLLDVVYKVIKRTAFKTLVDEVQELE
                     AKSQDPGAVVLGKSLTERAQLLKNVFKKNHVNLYRSESLQQNLLHGYAFSQDENGIVS
                     QSEVIRAYDTTKQRPDEW"
     exon            282..396
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            397..496
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            497..595
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            596..641
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            642..682
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            683..756
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            757..826
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            827..954
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1066
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1232
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1360
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1438
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1527
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1572
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1645
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1646..1751
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1834
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1884
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..1954
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2039
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2179
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2318
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2383
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2556
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2740
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2741..2851
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2852..2926
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3049
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..3128
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3129..3190
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3191..3247
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3355
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3444
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3445..3537
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3538..3629
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..8270
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8247..8252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /note="hexamer: AATAAA"
     polyA_site      8270
                     /gene="ATP8A1"
                     /gene_synonym="ATPASEII; ATPIA; ATPP2"
                     /note="major polyA site"
ORIGIN      
        1 aagagctcgc ccagctctgc gggcgccgcc accttcgccg ccaccgctgc ctttctcctc
       61 ctcctgtcgg cgtgcggggg ccgcgcccgg cggcagctct gccctaggtg ggcggcggcg
      121 cggcccaggc tgcagctgag cgctctgcgc ggcgcagccg ggtctcccgc gtgtaccacg
      181 ccgtgacagg tgcagagtcc gggctgagga cccacctgca gccgccgccg cgatgcccac
      241 catgcggagg accgtgtcgg agatccgctc gcgcgccgaa ggttatgaga agacagatga
      301 tgtttcagag aagacctcac tggctgacca ggaggaagta aggactattt tcatcaacca
      361 gccccagctg acaaaattct gcaataacca tgtcagcact gcaaaataca acataatcac
      421 attccttcca agatttctct actctcagtt cagaagagct gctaattcat tttttctctt
      481 tattgcactg ctgcagcaaa tacctgatgt gtcaccaaca ggtcgttata caacactggt
      541 tcctctctta tttattttag ctgtggcagc tatcaaagag ataatagaag atattaaacg
      601 acataaagct gataatgcag tgaacaagaa acaaacgcaa gttttgagaa atggtgcttg
      661 ggaaattgtc cactgggaaa aggtggcagt aggggagata gtgaaagtga ccaatgggga
      721 acatctccca gcagatctca tcagtctgtc ctcaagtgag ccccaagcca tgtgctacat
      781 tgaaacatcc aacttagatg gtgaaacaaa cttgaaaatt agacagggct taccagcaac
      841 atcagatatc aaagacgttg acagtttgat gaggatttct ggcagaattg agtgtgaaag
      901 tccaaacaga catctctacg attttgttgg aaacataagg cttgatggac atggcaccgt
      961 tccactggga gcagatcaga ttcttcttcg aggagctcag ttgagaaata cacagtgggt
     1021 tcatggaata gttgtctaca ctggacatga caccaagctg atgcagaatt caacaagtcc
     1081 accacttaag ctctcaaatg tggaacggat tacaaatgta caaattttga ttttattttg
     1141 tatcttaatt gccatgtctc ttgtctgttc tgtgggctca gccatttgga atcgaaggca
     1201 ttctggaaaa gactggtatc tcaatctaaa ctatggtggc gctagtaatt ttggactgaa
     1261 tttcttgacc ttcatcatcc ttttcaacaa tctcattcct atcagcttat tggttacatt
     1321 agaagttgtg aaatttaccc aggcatactt cataaattgg gatcttgaca tgcactatga
     1381 acccacagac actgctgcta tggctcgaac atctaatctg aatgaggaac ttggccaggt
     1441 taaatacata ttttctgaca aaactggtac tctgacatgc aatgtaatgc agtttaagaa
     1501 gtgcaccata gcgggagttg cttatggcca tgtccctgaa cctgaggatt atggctgctc
     1561 tcctgatgaa tggcagaact cacagtttgg agatgaaaaa acatttagtg attcatcatt
     1621 gctggaaaat ctccaaaata atcatccaac tgcacctata atatgtgaat ttcttacaat
     1681 gatggcagtc tgtcacacag cagtgccaga gcgagaaggt gacaagatta tttatcaagc
     1741 agcatctcca gatgagggag cattggtcag agcagccaag caattgaatt ttgttttcac
     1801 tggaagaaca cccgactcgg tgattataga ttcactgggg caggaagaaa gatatgaatt
     1861 gctcaatgtc ttggagttta ccagtgctag gaaaagaatg tcagtgattg ttcgcactcc
     1921 atctggaaag ttacgactct actgcaaagg agctgacact gtaatttatg atcgactggc
     1981 agagacgtca aaatacaaag aaattaccct aaaacattta gagcagtttg ctacagaagg
     2041 gttaagaact ttatgttttg ctgtggctga gatttcagag agcgactttc aggagtggcg
     2101 agcagtctat cagcgagcat ctacatctgt gcagaacagg ctactcaaac tcgaagagag
     2161 ttatgagttg attgaaaaga atcttcagct acttggagca acagccattg aggataaatt
     2221 acaagatcaa gtgcctgaaa ccatagaaac gctaatgaaa gcagacatca aaatctggat
     2281 ccttacaggg gacaagcaag aaactgccat taacatcgga cactcctgca aactgttgaa
     2341 gaagaacatg ggaatgattg ttataaatga aggctctctt gatggaacaa gggaaactct
     2401 cagtcgtcac tgtactaccc ttggtgatgc tctccggaaa gagaatgatt ttgctcttat
     2461 aattgatggg aaaaccctca aatatgcctt aacctttgga gtacgacagt atttcctgga
     2521 cttagctttg tcatgcaaag ctgtcatttg ctgtcgggtt tctcctcttc aaaaatctga
     2581 agttgttgag atggttaaga aacaagtcaa agtcgtaacg cttgcaatcg gtgatggagc
     2641 aaatgatgtc agcatgatac agacagcgca cgttggtgtt ggtatcagtg gcaatgaagg
     2701 cctgcaggca gctaattcct ctgactactc catagctcag ttcaaatatt tgaagaattt
     2761 actgatgatt catggtgcct ggaactataa cagagtctcc aagtgcatct tatactgctt
     2821 ctacaagaat atagtgctct atattatcga gatctggttt gcctttgtta atggcttttc
     2881 tggacagatc ctctttgaaa gatggtgtat aggtctctat aacgtgatgt ttacagcaat
     2941 gcctccttta actcttggaa tatttgagag atcatgcaga aaagagaaca tgttgaagta
     3001 ccctgaatta tacaaaacat ctcagaatgc cctggacttc aacaccaagg ttttctgggt
     3061 tcattgttta aatggcctct tccactcagt tattctgttt tggtttccac taaaagccct
     3121 tcagtatggt actgcatttg gaaatgggaa aacctcggat tatctgctac tgggaaactt
     3181 tgtgtacact tttgtggtga taactgtgtg tttgaaagct ggattggaga catcatattg
     3241 gacatggttc agccacatag cgatatgggg gagcatcgca ctctgggtgg tgttttttgg
     3301 aatctactca tctctgtggc ctgccattcc gatggcccct gatatgtcag gagaggcagc
     3361 catgttgttc agttctggag tcttttggat gggcttgtta ttcatccctg tggcatctct
     3421 gctccttgat gtggtgtaca aggttatcaa gaggactgct tttaaaacat tggtcgatga
     3481 agttcaggag ctggaggcaa aatctcaaga cccaggagca gttgtacttg gaaaaagcct
     3541 gaccgagagg gcgcaactgc tcaagaacgt ctttaagaag aaccacgtga acttgtaccg
     3601 ctctgaatcc ttgcaacaaa atctgctcca tgggtatgcg ttctctcaag atgaaaatgg
     3661 aatcgtttca cagtctgaag tgataagagc atatgatacc acgaaacaga ggcccgacga
     3721 atggtgatgg ggagagcctg aaaggcaggc tctgttacct ctctaaggag agctaccagg
     3781 ttgtcaccgc agtctgctaa ccaattccag tctggtccat gaagaggaaa ggtagatctg
     3841 agctcatctc gctgatggac attcagattc atgtatatta tagacataag cactgtgcaa
     3901 ctgtactgta acaccatctc ttttggattt ttttaaggta tttgctaagt ctttgtaaac
     3961 ggaaattgaa aatgacctgg tatcttgcca gagggctttc ttaaacggag aataagtcag
     4021 tattcttatg ccattactgt ggggctgtaa ctgactgtca gtttattggc tgtaccacaa
     4081 ggtaaccaac cattaaaaaa ctctaaatga tatttagtta aagggactct tggtatccag
     4141 acttagattt caggatatgc tgaaacaaac cagcattctt aaggaactga ctcaccttcc
     4201 tgagcaaaat ttctaaacaa gcatttgtgt ccaaaattgt cttgataaat gtttgccaaa
     4261 gaggttcagt aagtgttttt ctagttcagt agtcatatgc ccagaaatgt aagagaaagt
     4321 ttacttccag ttccgctgta agatctgcat gcctgacttt ccaaatgtaa gagtgattta
     4381 caaaaatgaa tatttcaagg catttgctac taaaatcggt gatgttgcac ctttggcctt
     4441 acaaatgctt ctttgttgtt tgtcgtgttt atttgttaga ggacacacgt gttaatgtga
     4501 ctctgttgtt atgacactga tttttcaaac tatgtatgtt tcaggtattt ctgatgaagt
     4561 ttcatcatca tttagatttt tctaaaaatc tggctaatgc agtagattga gtgatgtcat
     4621 tttgtcttaa agtttttcct cttaagaaac atatgctacg tatttacgtg ggatttccaa
     4681 agcttctgtt gcaatatttg gaataacatg tcagataaat gcatgggctt ttgtcctgtg
     4741 ttccagttcc cactagagat gcctgtgtct tgtgtagcac acccagtgtt atggtgactg
     4801 ccccctatac tgaagactga aaattatttc acagttcact catcaaatag ttcccaaaat
     4861 tcgtcacatg ctgcttattg ggacaaatag gtagtacatt ttccccattt aaaaaatgcg
     4921 gattttactc aggccggtaa ctttacagtc agaggacacg ttcatcatga gtagcttttg
     4981 ttagtatgtt ttaaaatgta tcttcagttc aattattttc agcatttaca agacatctga
     5041 aaatggctat tttgctacca acagtaaatg aaggggctgt ttaaaaacca caaccagttt
     5101 tctacactat tttttaaata atactttcat ttgaaaaaaa ggaattagtt ttcagataca
     5161 cttcagagat tgaagcaaac tatttgcctt ttactcaaaa gcctgcttgc ctttacatgg
     5221 acttaccagc aaaataggta gaactttctc ttttaaaaaa agtcaactag aattgagaag
     5281 aggtgatttt ttttcagatc gcttctcgag tttaatattt tcacattctt ttcacccttt
     5341 ttctcaatct agatttaaaa ttaggatata tgtcatttcc ttgtctgtat ttgtagctcc
     5401 ttagttacca gtatgcctct ccattttcta caaataagag gttataacac atatacataa
     5461 ttctaacctt aagggaacac acgtttacat actttacttc ccaagccctt cctgtttggg
     5521 gtacagattg agagagtcat gaatcaacac atctagcaag accacaggtg taagagtcta
     5581 agatcgtctt caaaattctg aagtcccagt ctttacctgt ccagtgaatg aatattcaga
     5641 gcagcttttc ctgggcttcc cagtggtgat agctgaggtc aaaccacaaa aaataagaaa
     5701 gcaagagtga aatgcacccc tccagagaaa cactttgtag tgtttaattc tgttaataga
     5761 gaagagctgc ttctgtttgc gctcacttca tcagtggcac ccttctgcag aattttaata
     5821 taaaaacatt atggatataa tagaactgga ttttctgact taaaaatgta agttttattt
     5881 taatcttgaa acgtggattg tttctgtgga gctcttaaac atgagaagaa tacttacggt
     5941 tgataatgtg taacatgatc tgaaatgtga ctaatttgag cctctttgtc ccatcgtcct
     6001 gtttttgaat tattgacatt gtcagtctct ttgcttcctg ggtgagactt ggggtttgag
     6061 ggacagggaa tgaccttctt ggtgaaactt aaaatataac attgcaattg cagtgacttt
     6121 acagtgttaa attagagaaa atagtctgat tttttaaacc ttccttaact ggaaaaaagt
     6181 cacatggttt taccaggatt gaaataaaca gtcaatgtga cttttaacat gtgttttttt
     6241 gaaataaagg gcacgtactc ttcaattaaa aagttcctta tagggactct ggcaaatgct
     6301 aacacagttg ctttacaatg tttacaattc agacaatacg acttataata gaaaatcctc
     6361 attcatttag cattgaaaag ctggaagttg cttctttaat gttgaatagt atacagtggt
     6421 attgagcatg gactttctaa atgttttata tatacatata aaaatatatt ggtgtctcac
     6481 acccagaaag atgttatatt gtagatatta ttaggaaaac agtgtttctc aggaacgttg
     6541 taaattttaa atgatatatg tacttcccgt cctcccacct ccactctgtg ctctaatgtg
     6601 agactgcttc agcagtgttg ctaagttaat ggaaaacttt ttctaatcaa gtcaggtgaa
     6661 tgtgtattct gctaaataat gttagccatt tacatgaatt gtatggtcat taaatggaat
     6721 cagtgattcc tctttaattt ccagagggga aatgaattat ggaaatcagt cagcattctg
     6781 atcattaaat tttatacttt aattttgccg ttcagcattc taaatatcca atgtgaaagt
     6841 cacatgataa tttgttttgc attgcgtgca ctgtacaaca cttacaactt gtcatttaaa
     6901 atgttttctc gggaaatgaa tgctagtcag aaagtaatag attgtattat tcatagtttt
     6961 aaaattatga caatgtcata attactacaa agctaaataa tcgtgtttat ttttgtgcag
     7021 ttgccctttg atagttcctg gttttaaaac ctattaagtg tataatctta caaatagtca
     7081 tctacaaaat ttatggagaa agtgcccagc ccattcacat cacatggacc aggaattctt
     7141 ttgtaaatga cttaaggtaa catcatgcag ttcagtgcct aataaatgct ttttaatgat
     7201 gaacatttct ataatgactc gtaagatacc atagtctgat ttttctcaca ttaaaataac
     7261 tgaagtcact tgtgtaacgt agttatactt tgctgcattt taattaacct tcaacagcta
     7321 ttaaagtgga atgtaagtta aattttgaag gaaaggaaat aaatgttttc catatttcgt
     7381 cttgatttac tttctgtatg agaacagctg tgtttttgat aggtttatgg tttgcatgag
     7441 ttcatattta aagtgatcca ggccaatgca tggctattgc tgtaaatctt gatgtttatt
     7501 tctgccttgt aaagttctat cacggcctac ctggaattta aaattcagta gacaaattaa
     7561 ttggtcctct gcacaacttt tttaataagt agattatttt acaaagaaat ttgaacaaat
     7621 ttaattgaat cttttgttta gcttgcctct aagaactttt cttaataaag ctcccaaaac
     7681 ttctcagcaa ataaatctcc cttaagtagg aaagctagat ttcatatttg cttactttga
     7741 attaacagca actttccaca ggtaaatctg ttcttgcaaa gatgtgagca gaatagttaa
     7801 aaataatatt tttatgtttc atggttctaa atggaagcca taaatgcagt aaatactatc
     7861 tgttgtttaa ctactttaat cgtcattttt tacattttca agtttattag gttaagaaaa
     7921 acagggcagc cttggaaggc agctactaca gaaaactgca gttttgcgtt aaagataaag
     7981 tagtattttc agctccctga aaaaccattc ctgctgaaac tgctgtagaa attgtgaagc
     8041 tgcatgagtg gagagtattg aatctgtggt tatagtagtt ttctcaggtt tgtttatctt
     8101 gatgtttgat gcactgtgtt ttatagttat taaaattgag taatattatt tctatgcagt
     8161 gttatgtgtc attggccttt tgtgaatgtg catgttttaa actgcaaatt ttaaacattt
     8221 tgtcctctaa ttgttattaa aaatgaaata aactttacca ttacttaaaa
//
