LOCUS       NM_000052               8492 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens ATPase copper transporting alpha (ATP7A), transcript
            variant 1, mRNA.
ACCESSION   NM_000052
VERSION     NM_000052.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Harkness,J.R., Thomas,H.B., Urquhart,J.E., Jamieson,P.,
            O'Keefe,R.T., Kingston,H.M., Deshpande,C. and Newman,W.G.
  CONSRTM   Genomics England Research Consortium
  TITLE     Deep intronic variant causes aberrant splicing of ATP7A in a family
            with a variable occipital horn syndrome phenotype
  JOURNAL   Eur J Med Genet 67, 104907 (2024)
   PUBMED   38141875
  REMARK    GeneRIF: Deep intronic variant causes aberrant splicing of ATP7A in
            a family with a variable occipital horn syndrome phenotype.
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Kaler,S.G.
  TITLE     ATP7A-related copper transport diseases-emerging concepts and
            future trends
  JOURNAL   Nat Rev Neurol 7 (1), 15-29 (2011)
   PUBMED   21221114
  REMARK    Review article
REFERENCE   3  (bases 1 to 8492)
  AUTHORS   Reddy,M.C., Majumdar,S. and Harris,E.D.
  TITLE     Evidence for a Menkes-like protein with a nuclear targeting
            sequence
  JOURNAL   Biochem J 350 Pt 3 (Pt 3), 855-863 (2000)
   PUBMED   10970802
REFERENCE   4  (bases 1 to 8492)
  AUTHORS   Reddy,M.C. and Harris,E.D.
  TITLE     Multiple transcripts coding for the menkes gene: evidence for
            alternative splicing of Menkes mRNA
  JOURNAL   Biochem J 334 (Pt 1) (Pt 1), 71-77 (1998)
   PUBMED   9693104
REFERENCE   5  (bases 1 to 8492)
  AUTHORS   Dierick,H.A., Ambrosini,L., Spencer,J., Glover,T.W. and Mercer,J.F.
  TITLE     Molecular structure of the Menkes disease gene (ATP7A)
  JOURNAL   Genomics 28 (3), 462-469 (1995)
   PUBMED   7490081
REFERENCE   6  (bases 1 to 8492)
  AUTHORS   Tumer,Z., Vural,B., Tonnesen,T., Chelly,J., Monaco,A.P. and Horn,N.
  TITLE     Characterization of the exon structure of the Menkes disease gene
            using vectorette PCR
  JOURNAL   Genomics 26 (3), 437-442 (1995)
   PUBMED   7607665
REFERENCE   7  (bases 1 to 8492)
  AUTHORS   Vulpe,C., Levinson,B., Whitney,S., Packman,S. and Gitschier,J.
  TITLE     Isolation of a candidate gene for Menkes disease and evidence that
            it encodes a copper-transporting ATPase
  JOURNAL   Nat Genet 3 (1), 7-13 (1993)
   PUBMED   8490659
  REMARK    Erratum:[Nat Genet 1993 Mar;3(3):273]
REFERENCE   8  (bases 1 to 8492)
  AUTHORS   Mercer,J.F., Livingston,J., Hall,B., Paynter,J.A., Begy,C.,
            Chandrasekharappa,S., Lockhart,P., Grimes,A., Bhave,M.,
            Siemieniak,D. et al.
  TITLE     Isolation of a partial candidate gene for Menkes disease by
            positional cloning
  JOURNAL   Nat Genet 3 (1), 20-25 (1993)
   PUBMED   8490647
REFERENCE   9  (bases 1 to 8492)
  AUTHORS   Chelly,J., Tumer,Z., Tonnesen,T., Petterson,A., Ishikawa-Brush,Y.,
            Tommerup,N., Horn,N. and Monaco,A.P.
  TITLE     Isolation of a candidate gene for Menkes disease that encodes a
            potential heavy metal binding protein
  JOURNAL   Nat Genet 3 (1), 14-19 (1993)
   PUBMED   8490646
REFERENCE   10 (bases 1 to 8492)
  AUTHORS   Kaler,S.G. and DiStasio,A.T.
  TITLE     ATP7A-Related Copper Transport Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301586
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL356235.22, DB164588.1,
            L06476.1, AB117973.1, AK299449.1, AL645821.2 and BU753163.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000052.6.
            
            Summary: This gene encodes a transmembrane protein that functions
            in copper transport across membranes. This protein is localized to
            the trans Golgi network, where it is predicted to supply copper to
            copper-dependent enzymes in the secretory pathway. It relocalizes
            to the plasma membrane under conditions of elevated extracellular
            copper, and functions in the efflux of copper from cells. Mutations
            in this gene are associated with Menkes disease, X-linked distal
            spinal muscular atrophy, and occipital horn syndrome.
            Alternatively-spliced transcript variants have been observed.
            [provided by RefSeq, Aug 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: L06133.1, SRR1803613.139233.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341514.11/ ENSP00000345728.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AL356235.22        38057-38060
            5-604               DB164588.1         1-600
            605-984             L06476.1           408-787
            985-3695            AB117973.1         949-3659
            3696-4735           AK299449.1         662-1701
            4736-8121           AL645821.2         77227-80612
            8122-8492           BU753163.1         12-382              c
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq21.1"
     gene            1..8492
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /note="ATPase copper transporting alpha"
                     /db_xref="GeneID:538"
                     /db_xref="HGNC:HGNC:869"
                     /db_xref="MIM:300011"
     exon            1..143
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            144..284
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..4667
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /EC_number="7.2.2.8"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Menkes disease-associated protein; copper pump 1;
                     copper-transporting ATPase 1; Cu++-transporting P-type
                     ATPase; ATPase, Cu++ transporting, alpha polypeptide"
                     /codon_start=1
                     /product="copper-transporting ATPase 1 isoform 1"
                     /protein_id="NP_000043.4"
                     /db_xref="CCDS:CCDS35339.1"
                     /db_xref="GeneID:538"
                     /db_xref="HGNC:HGNC:869"
                     /db_xref="MIM:300011"
                     /translation="MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLE
                     EKNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPDPLPVLTDTLFLTVTASLTLPWDH
                     IQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKSGAC
                     EDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQEATIVYQPH
                     LISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTND
                     STATFIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKA
                     IEAVSPGLYRVSITSEVESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCV
                     QSIEGVISKKPGVKSIRVSLANSNGTVEYDPLLTSPETLRGAIEDMGFDATLSDTNEP
                     LVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKCYIQVTGMTCASCVANIER
                     NLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENADEGDGVL
                     ELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIES
                     LGFEASLVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLH
                     HNQNMSKEEMINLHSSMFLERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALK
                     HKTANMDVLIVLATTIAFAYSLIILLVAMYERAKVNPITFFDTPPMLFVFIALGRWLE
                     HIAKGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDIIKVVPGGKF
                     PVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLS
                     QIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFP
                     GYNRSISRTETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGE
                     PLEMAHKVKVVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPL
                     GTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNAS
                     LVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMT
                     EHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTAR
                     SIASQVGITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGT
                     GTDVAIEAADVVLIRNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFM
                     PIGLVLQPWMGSAAMAASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISV
                     HVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFRE
                     DDDTAL"
     exon            285..774
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            775..1500
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1707
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            1708..1871
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..2033
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2110
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2336
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2337..2570
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2662
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2790
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2945
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..3080
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            3081..3275
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            3276..3458
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3675
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            3676..3822
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            3823..3965
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            3966..4169
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            4170..4287
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            4288..4390
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     exon            4391..8492
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8023..8028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /note="hexamer: AATAAA"
     polyA_site      8045
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /note="major polyA site"
     regulatory      8474..8479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /note="hexamer: ATTATA"
     polyA_site      8492
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /note="major polyA site"
ORIGIN      
        1 aggagttgtt gctgccgccg ccgcagccgc agctactgtg acttctccga ttgtgtgagc
       61 tttgttggag cctgcgtacg tggatttatc gctgccacgg tctgcgtagc tccagaggtt
      121 taaccatagg atagagaaac caggaatgta atgaggaaat caaaatggat ccaagtatgg
      181 gtgtgaattc tgttaccatt tctgttgagg gtatgacttg caattcctgt gtttggacca
      241 ttgagcagca gattggaaaa gtgaatggtg tgcatcacat taaggtatca ctggaagaaa
      301 aaaatgcaac tattatttat gaccctaaac tacagactcc aaagacccta caggaagcta
      361 ttgatgacat gggctttgat gctgttatcc ataatcctga ccctctccct gttttaactg
      421 acaccttgtt tctgactgtt acggcgtcac tgactttgcc atgggaccat atccaaagca
      481 cattgctgaa gaccaagggt gtgacagaca ttaaaattta ccctcagaaa agaactgtag
      541 cagtgacaat aatcccttct atagtgaatg ccaatcagat aaaagagctg gttccagaac
      601 tcagtttaga tactgggaca ctggagaaaa agtcaggagc ttgtgaagat catagtatgg
      661 ctcaagctgg tgaagtcgtg ctgaagatga aagtggaagg gatgacctgc cattcatgta
      721 ctagcactat tgaaggaaaa attgggaaac tgcaaggtgt tcagcgaatt aaagtctccc
      781 tggacaatca agaagctact attgtttatc aacctcatct tatctcagta gaggaaatga
      841 aaaagcagat tgaagctatg ggctttccag catttgtcaa aaagcagccc aagtacctca
      901 aattgggagc tattgatgta gaacgtctaa agaacacacc agttaaatcc tcagaagggt
      961 cacagcaaag gagtccatca tataccaatg attcaacagc cactttcatc attgatggca
     1021 tgcattgtaa atcatgtgtg tcaaatattg aaagtacttt atctgcactc caatatgtaa
     1081 gcagcatagt agtttcttta gagaataggt ctgccattgt gaagtataat gcaagctcag
     1141 tcactccaga atccctgaga aaagcaatag aggctgtatc accggggcta tatagagtta
     1201 gtatcacaag tgaagttgag agtacctcaa actctccctc cagctcatct cttcagaaga
     1261 ttcctttgaa tgtagttagc cagcctctga cacaagaaac tgtgataaac attgatggca
     1321 tgacttgtaa ttcctgtgtg cagtctattg agggtgtcat atcaaaaaag ccaggtgtaa
     1381 aatccatacg agtctccctt gcaaatagca atgggactgt tgagtatgat cctctactaa
     1441 cctctccaga aacgttgaga ggagcaatag aagacatggg atttgatgct accttgtcag
     1501 acacgaatga gccgttggta gtaatagctc agccttcatc ggaaatgccg cttttgactt
     1561 caactaatga attttatact aaagggatga caccagttca agacaaggag gaaggaaaga
     1621 attcatctaa gtgttacata caggtcactg gcatgacttg cgcttcctgt gtagcaaaca
     1681 ttgaacggaa tttaaggcgg gaagaaggaa tatattctat acttgtggcc ctgatggctg
     1741 gcaaggcaga agtaaggtat aatcctgctg ttatacaacc cccaatgata gcagagttca
     1801 tccgagaact tggatttgga gccactgtga tagaaaatgc tgatgaagga gatggtgttt
     1861 tggaacttgt tgtgagggga atgacgtgtg cctcctgcgt acataaaata gagtctagtc
     1921 tcacaaaaca cagagggatc ctatactgct ccgtggccct ggcaaccaac aaagcacata
     1981 ttaaatatga cccagaaatt attggtccta gagatattat ccatacaatt gaaagcttag
     2041 gttttgaagc ttctttggtc aagaaggatc ggtcagcaag tcacttagat cataaacgag
     2101 aaataagaca atggagacgg tcttttcttg tgagtctgtt tttctgtatt cctgtaatgg
     2161 ggctgatgat atatatgatg gttatggacc accactttgc aactcttcac cataatcaaa
     2221 acatgagtaa agaagaaatg atcaaccttc attcttctat gttcctggag cgccagattc
     2281 ttccaggatt gtctgttatg aatttgctgt cctttttatt gtgtgtacct gtacagtttt
     2341 tcggaggctg gtacttctac attcaggctt ataaagcact gaagcataag acagcaaata
     2401 tggacgtact gattgtgctg gcaaccacca ttgcatttgc ctactctttg attattcttc
     2461 tagttgcaat gtatgagaga gccaaagtga accctattac tttctttgac acacccccta
     2521 tgctgtttgt gtttattgca ctaggccgat ggctggaaca tatagcaaag ggcaaaacat
     2581 cagaggctct tgcaaagtta atttcactac aagctacaga agcaactatt gtaactcttg
     2641 attctgataa tatcctcctc agtgaagaac aagtggatgt ggaacttgta caacgtggag
     2701 atatcattaa agtagttcca ggaggcaaat ttccagtgga tggtcgtgtt attgaaggac
     2761 attctatggt agatgagtcc ctcatcacag gggaggcaat gcctgtggct aagaaacctg
     2821 gcagcacagt gattgctggt tccattaacc agaacgggtc actgcttatc tgcgcaacac
     2881 atgttggagc agacacaacc ctttctcaaa ttgtcaaact tgtggaagag gcacaaacat
     2941 caaaggctcc tatccagcag tttgcagaca aactcagtgg ctattttgtt ccttttattg
     3001 tttttgtttc cattgccacc ctcttggtat ggattgtaat tggatttctg aattttgaaa
     3061 ttgtggaaac ctactttcct ggctacaata gaagtatctc ccgaacagaa acgataatac
     3121 gatttgcttt ccaagcctct atcacagttc tgtgtattgc atgtccctgt tcactgggac
     3181 tggccactcc aactgctgtg atggtgggta caggagtagg tgctcaaaat ggcatactaa
     3241 taaaaggtgg agagccattg gagatggctc ataaggtaaa ggtagtggta tttgataaga
     3301 ctggaaccat tactcacgga accccagtgg tgaatcaagt aaaggttcta actgaaagta
     3361 acagaatatc acaccataaa atcttggcca ttgtgggaac tgctgaaagt aacagtgaac
     3421 accctctagg aacagccata accaaatatt gcaaacagga gctggacact gaaaccttgg
     3481 gtacctgcat agatttccag gttgtgccag gctgtggtat tagctgtaaa gtcaccaata
     3541 ttgaaggctt gctacataag aataactgga atatagagga caataatatt aaaaatgcat
     3601 ccctggttca aattgatgcc agtaatgaac agtcatcaac ttcgtcttcc atgattattg
     3661 atgcccagat ctcaaatgct cttaatgctc agcagtataa agtcctcatt ggtaaccggg
     3721 agtggatgat tagaaatggt cttgtcatta ataacgatgt aaatgatttc atgactgaac
     3781 atgagagaaa aggtcggact gctgtattag tagcagttga tgatgagctg tgtggcttga
     3841 tagccattgc agacacagtg aagcctgaag cagaactggc tatccatatt ctgaaatcta
     3901 tgggcttaga agtagttctg atgactggag acaacagtaa aacagctaga tctattgctt
     3961 ctcaggttgg cattactaag gtgtttgctg aagttctacc ttctcacaag gttgctaaag
     4021 tgaagcaact tcaagaggag gggaaacggg tagcaatggt gggagatgga atcaatgact
     4081 ccccagctct ggcaatggct aatgtgggaa ttgctattgg cacaggcaca gatgtagcca
     4141 ttgaagcagc tgatgtggtt ttgataagga atgatcttct ggatgtagtg gcaagtattg
     4201 acttatcaag aaagacagtc aagaggattc ggataaattt tgtctttgct ctaatttata
     4261 atctggttgg aattcccata gctgctggag tttttatgcc cattggtttg gttttgcagc
     4321 cctggatggg atctgcagca atggctgctt catctgtttc tgtagtactt tcttctctct
     4381 tccttaaact ttacaggaaa ccaacttacg agagttatga actgcctgcc cggagccaga
     4441 taggacagaa gagtccttca gaaatcagcg ttcatgttgg aatagatgat acctcaagga
     4501 attctcctaa actgggtttg ctggaccgga ttgttaatta tagcagagcc tctataaact
     4561 cactactgtc tgataaacgc tccctaaaca gtgttgttac cagtgaacct gacaagcact
     4621 cactcctggt gggagacttc agggaagatg atgacactgc attataaaag gccatggaga
     4681 gtgctgccag tttaacttgt catgcactga cacagcattc atgatgttac cttcactttt
     4741 caaaatattg tagaaggatt tttctcatgc tcttatatta gggattctat ttgagttgcg
     4801 tttatctgtt ggcaaaaata tctttttcaa ggcatcagct ctgaacctag ctttatttaa
     4861 actgaatttc cagtatattt ttgttttcac taacaacaga taaggtagag cagtgaggtt
     4921 tacaacaagc cctacaatta gagattgctg aactgctgct aaagtgattt tttttttatt
     4981 tgaccaaaaa aaaaaaggcc caagaagaag aaaatgaaaa atttgaagat ttgagagcat
     5041 gaagatattc atgcttttga actcaaaata ttgaagatac tctcaagcct gtatccctgc
     5101 cccactgggg agcaatgact ttcaaagcac tgtgtataaa acatctagtt ttagaaggga
     5161 aacagttgaa actgtttaaa aatagatgtg ccttatttat tgcaggcttt ctttccccca
     5221 ttctccctgc atccttgtcc ttgcaggtgc ttttttagat gctccaatat gtcttctttt
     5281 gttattttct ttcgagctaa ccaagtttag gtggtttttc attgattaaa aataactgac
     5341 aactgttcta atattttgct cctttttaaa ttttgtagct caaaagacct taaaggtctg
     5401 tagggttccc tgcctcccat ctttccactg ttgtaaaaag tatatcaaat tattccttca
     5461 agtttcctag ctctgtgctc agtttcagtt cactcctgcc aagttggact ctaagttatt
     5521 cttcatgtag tctgctgatc tcagtctgga aacttaacat tatgagcctt ttctgctcaa
     5581 aaaattttca aagattaaaa ctattataca tatacaggtc atataaaatt acctggattc
     5641 actaaatttg tttgttgttg ttgttgttgt tgttgttgtt gttgagacag agtcttgttt
     5701 tgcagcccag gctggagtgc agtggcacca tcttggctca ctgcaacctc tgcctaccgg
     5761 attcaaggaa ttctccctgc ctcagcctcc tgagtagcta ggattacagg tgcctgccac
     5821 cacacccggc taattttcat atttttcagt agagacgggg tttcgccatg ttggctagcc
     5881 tggtcttaaa ctcctaacct caggtgatcc acccgccttg gcctcccaaa gtgctgggat
     5941 tacaggtgtg agcctccatg cccagcctaa atttgtattt tttgaattga gtataatcac
     6001 tttgctagta tatataattt aatagatttt atttatcttt tagtgtttca gataacctct
     6061 caaaaagact ttaaaataat gctattacac aaagctgcat ttaccaaaaa atacagtaaa
     6121 atcataatac agaaactaaa atttccctag gttatgacgc tttttagcta aatatatact
     6181 cttctctagt ttaaaacatt tgaacttgcc tagttagtgt ggttggcaaa tttaggagct
     6241 tgttcccatt gccaaatgga tttagaaatt cccttgtgag tgcctggtag ctaatacact
     6301 ggtcagagat ctggtacttg taagactatt taaatttctt tgttagttgc aagatggatt
     6361 tcatatgcag aatatgtaaa tgaagaggac tcataagtaa attcctaaca ttttgttccc
     6421 attaccagaa gcaaagctgc tgctaaccca acatctggca cataggattt gtactcggta
     6481 aatgttagtt cttttctccc cttgaggtca gtaataaata caaaaaaatc atttttctag
     6541 agcagagtct taaaatcagg tgggggtagg ggatggagtt cttcctttcc tacccctttt
     6601 ctcttttatc ctttcatata tacacatgca aagtttacaa ccttattcca tgctgtcttt
     6661 cagatttaga aaagatctaa tttctgtctc agctgtctta aagagagaac tgaagctttt
     6721 gatgaaggtg ctattaatct agaaaggcaa acccatttca ctgaaatatc aatgggtttg
     6781 catatctagg cccttttttt agaccaatgc ctatgccatc ctccatgctt tcagtttgag
     6841 ttttattatt tattatttta attccagtgg cccatcttat aatacaactt gtttcttcta
     6901 gaagacagag ctgatagggt aaatgttgaa aaaagaagca tgcctccttc tccctcccac
     6961 ccacctcaag cagttgaaca cagccagtta ttcttccatt attatgtgta ccttggagtc
     7021 atcctcttgg tcttgtattc atattgtggg acagtgggaa tagcagcttg tagtattgaa
     7081 ataatcaaag agataatttc agctcttaca acaagaacag aaaacatgct aattagaaaa
     7141 agtcctgctt taagtaatat gtagccacat ttgaatcctc taccacaagt cttttttgca
     7201 atcttgaact ttcattagct tcaaagagaa gctgtattta caggagaaag aggtgattat
     7261 ggagggaatc aaaaatactg ctttcagtta gtagctagct ttaagtcagg agttagtaat
     7321 gagaaatttt ataaatgtgt atttctgtgt attcacatac atatatatat acacacatat
     7381 atatgtacat acacatacat acatattacc atactgaagg gaaatggatt tatatttgaa
     7441 tttgatattt gaatatttga agttctctat ttatatttca tttatcactc ttctgtattc
     7501 actcagcaac catgccctta gtctgaagat aacaaggata ctttaatatc cagtgccggt
     7561 tcagactcac ctatgtggca ccttaaactt aaatatccaa agatgccttt tgaatttcaa
     7621 agattaaaac acagctagag tagtagtatt gtagtctcaa gatgtatggt gtgtcatttg
     7681 tgaaaataga aacgttattt ttcctagttt agtatcaaca ttttagaatg tcaaatttga
     7741 tgccttgtga acaagtaatt ttatattgtg ctttaatttt ttaaaaagta ttctttattc
     7801 atatgtatag aatgcttaaa atagcactgt agacaagatg tttccaaaac tttaagtgcg
     7861 catatctttt ctgtatacag cttaaaatca aaaatgtatg ttaagagaat tttttctatt
     7921 acttatggat catgctttaa aatgattttt cttgatattt attttactcc attttgtttt
     7981 tttctctggg tgaggcatct ggttactggt tattttaaaa agaataaaga cattcctgga
     8041 atcactcctt ttgggttttt gtttgttttg tttcttttct acatttttaa gtattatttt
     8101 acaaaataga aaaaatatag attcatgcca aattattacc tatttttaca ctaactttct
     8161 gcagtccctc catctggtat gagtaaccag atagagcaca aagcatgagt tcttgttctt
     8221 cagttaaaag agcttctttc acagtgttga taacaaatgc cttttgtagc caaaaccagg
     8281 cgtctcaacc ttacgttttt agttaaagaa atgtttagct aaacgttgtt gaacattgat
     8341 tgtttggtac cgaaaacagc agtggacgat gttgtgcaat atccatctac tgtagttaag
     8401 atattcagta gtttgttttt cataagcatg taattgatca tatttctgcc aaggatgtgc
     8461 cttcaacttt ataattatag tgttgtaaaa ta
//
