LOCUS       NM_004231                678 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens ATPase H+ transporting V1 subunit F (ATP6V1F),
            transcript variant 1, mRNA.
ACCESSION   NM_004231
VERSION     NM_004231.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 678)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 678)
  AUTHORS   Wang,L., Wu,D., Robinson,C.V., Wu,H. and Fu,T.M.
  TITLE     Structures of a Complete Human V-ATPase Reveal Mechanisms of Its
            Assembly
  JOURNAL   Mol Cell 80 (3), 501-511 (2020)
   PUBMED   33065002
REFERENCE   4  (bases 1 to 678)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 678)
  AUTHORS   Vasanthakumar,T. and Rubinstein,J.L.
  TITLE     Structure and Roles of V-type ATPases
  JOURNAL   Trends Biochem Sci 45 (4), 295-307 (2020)
   PUBMED   32001091
  REMARK    Review article
REFERENCE   6  (bases 1 to 678)
  AUTHORS   Finbow,M.E. and Harrison,M.A.
  TITLE     The vacuolar H+-ATPase: a universal proton pump of eukaryotes
  JOURNAL   Biochem J 324 (Pt 3) (Pt 3), 697-712 (1997)
   PUBMED   9210392
  REMARK    Review article
REFERENCE   7  (bases 1 to 678)
  AUTHORS   Stevens,T.H. and Forgac,M.
  TITLE     Structure, function and regulation of the vacuolar (H+)-ATPase
  JOURNAL   Annu Rev Cell Dev Biol 13, 779-808 (1997)
   PUBMED   9442887
  REMARK    Review article
REFERENCE   8  (bases 1 to 678)
  AUTHORS   Fujiwara,T., Kawai,A., Shimizu,F., Hirano,H., Okuno,S., Takeda,S.,
            Ozaki,K., Shimada,Y., Nagata,M., Watanabe,T. et al.
  TITLE     Cloning, sequencing and expression of a novel cDNA encoding human
            vacuolar ATPase (14-kDa subunit)
  JOURNAL   DNA Res 2 (3), 107-111 (1995)
   PUBMED   8581736
REFERENCE   9  (bases 1 to 678)
  AUTHORS   Cross,S.H., Charlton,J.A., Nan,X. and Bird,A.P.
  TITLE     Purification of CpG islands using a methylated DNA binding column
  JOURNAL   Nat Genet 6 (3), 236-244 (1994)
   PUBMED   8012384
REFERENCE   10 (bases 1 to 678)
  AUTHORS   Dautry-Varsat,A.
  TITLE     Receptor-mediated endocytosis: the intracellular journey of
            transferrin and its receptor
  JOURNAL   Biochimie 68 (3), 375-381 (1986)
   PUBMED   2874839
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC107854.1 and BU683155.1.
            
            On Nov 22, 2018 this sequence version replaced NM_004231.3.
            
            Summary: This gene encodes a component of vacuolar ATPase
            (V-ATPase), a multisubunit enzyme that mediates acidification of
            eukaryotic intracellular organelles. V-ATPase dependent organelle
            acidification is necessary for such intracellular processes as
            protein sorting, zymogen activation, receptor-mediated endocytosis,
            and synaptic vesicle proton gradient generation. V-ATPase is
            composed of a cytosolic V1 domain and a transmembrane V0 domain.
            The V1 domain consists of three A and three B subunits, two G
            subunits plus the C, D, E, F, and H subunits. The V1 domain
            contains the ATP catalytic site. The V0 domain consists of five
            different subunits: a, c, c', c', and d. Additional isoforms of
            many of the V1 and V0 subunit proteins are encoded by multiple
            genes or alternatively spliced transcript variants. This encoded
            protein is the V1 domain F subunit protein. [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (1) is the predominant transcript.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189652.181791.1, D49400.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000249289.5/ ENSP00000249289.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-674               BC107854.1         31-704
            675-678             BU683155.1         18-21               c
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7q32.1"
     gene            1..678
                     /gene="ATP6V1F"
                     /gene_synonym="ATP6S14; VATF; Vma7"
                     /note="ATPase H+ transporting V1 subunit F"
                     /db_xref="GeneID:9296"
                     /db_xref="HGNC:HGNC:16832"
                     /db_xref="MIM:607160"
     exon            1..207
                     /gene="ATP6V1F"
                     /gene_synonym="ATP6S14; VATF; Vma7"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..409
                     /gene="ATP6V1F"
                     /gene_synonym="ATP6S14; VATF; Vma7"
                     /EC_number="7.1.2.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ATPase, vacuolar, 14 kD; V-ATPase F subunit; vacuolar ATP
                     synthase subunit F; ATPase, H+ transporting, lysosomal
                     14kDa, V1 subunit F; vacuolar proton pump F subunit;
                     V-ATPase 14 kDa subunit; adenosinetriphosphatase 14k
                     chain; H(+)-transporting two-sector ATPase, 14kD subunit;
                     V-type proton ATPase subunit F; V-ATPase subunit F;
                     vacuolar proton pump subunit F"
                     /codon_start=1
                     /product="V-type proton ATPase subunit F isoform 1"
                     /protein_id="NP_004222.2"
                     /db_xref="CCDS:CCDS5807.1"
                     /db_xref="GeneID:9296"
                     /db_xref="HGNC:HGNC:16832"
                     /db_xref="MIM:607160"
                     /translation="MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTT
                     INEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAK
                     DSILRRARGMFTAEDLR"
     exon            208..678
                     /gene="ATP6V1F"
                     /gene_synonym="ATP6S14; VATF; Vma7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      651..656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP6V1F"
                     /gene_synonym="ATP6S14; VATF; Vma7"
                     /note="hexamer: ATTAAA"
     polyA_site      678
                     /gene="ATP6V1F"
                     /gene_synonym="ATP6S14; VATF; Vma7"
                     /note="major polyA site"
ORIGIN      
        1 gtttcagtgg cttctggtgc tctagggtga gctctgcccg gctgcaggga tggcggggag
       61 gggtaagctc atcgcagtga tcggagacga ggacacggtg actggtttcc tgctgggcgg
      121 cataggggag cttaacaaga accgccatcc caatttcctg gtggtggaga aggatacaac
      181 catcaatgag atcgaagaca ctttccggca atttctaaac cgggatgaca ttggcatcat
      241 cctcatcaac cagtacatcg cagagatggt gcggcatgcc ctggacgccc accagcagtc
      301 catccccgct gtcctggaga tcccctccaa ggagcaccca tatgacgccg ccaaggactc
      361 catcctgcgc agggccaggg gcatgttcac tgccgaagac ctgcgctagg ggactcctca
      421 tagccctcag cccttccctc gtttccaggc ctctccccag gcttgccatc agccttcttt
      481 actttttgag cctctgattt ccaattccct gctccttccc actccattaa gaggctaggt
      541 gaggcgcttc taggttgctg gggctctgct ggttaaggaa caggaagcct gaccatctcc
      601 ctccactacc tcttccctgt gctgttacac agtgtcattg ttgatgttaa attaaagtca
      661 tattcttgct tctctcca
//
