LOCUS       NM_004047                987 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ATPase H+ transporting V0 subunit b (ATP6V0B),
            transcript variant 1, mRNA.
ACCESSION   NM_004047
VERSION     NM_004047.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Wang,L., Wu,D., Robinson,C.V., Wu,H. and Fu,T.M.
  TITLE     Structures of a Complete Human V-ATPase Reveal Mechanisms of Its
            Assembly
  JOURNAL   Mol Cell 80 (3), 501-511 (2020)
   PUBMED   33065002
REFERENCE   2  (bases 1 to 987)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 987)
  AUTHORS   Vasanthakumar,T. and Rubinstein,J.L.
  TITLE     Structure and Roles of V-type ATPases
  JOURNAL   Trends Biochem Sci 45 (4), 295-307 (2020)
   PUBMED   32001091
  REMARK    Review article
REFERENCE   4  (bases 1 to 987)
  AUTHORS   Hohn,A., Sittig,A., Jung,T., Grimm,S. and Grune,T.
  TITLE     Lipofuscin is formed independently of macroautophagy and lysosomal
            activity in stress-induced prematurely senescent human fibroblasts
  JOURNAL   Free Radic Biol Med 53 (9), 1760-1769 (2012)
   PUBMED   22982048
REFERENCE   5  (bases 1 to 987)
  AUTHORS   Adachi,K., Oiwa,K., Nishizaka,T., Furuike,S., Noji,H., Itoh,H.,
            Yoshida,M. and Kinosita,K. Jr.
  TITLE     Coupling of rotation and catalysis in F(1)-ATPase revealed by
            single-molecule imaging and manipulation
  JOURNAL   Cell 130 (2), 309-321 (2007)
   PUBMED   17662945
REFERENCE   6  (bases 1 to 987)
  AUTHORS   Forgac,M.
  TITLE     Structure and properties of the vacuolar (H+)-ATPases
  JOURNAL   J Biol Chem 274 (19), 12951-12954 (1999)
   PUBMED   10224039
  REMARK    Review article
REFERENCE   7  (bases 1 to 987)
  AUTHORS   Nelson,N. and Harvey,W.R.
  TITLE     Vacuolar and plasma membrane proton-adenosinetriphosphatases
  JOURNAL   Physiol Rev 79 (2), 361-385 (1999)
   PUBMED   10221984
  REMARK    Review article
REFERENCE   8  (bases 1 to 987)
  AUTHORS   Nishigori,H., Yamada,S., Tomura,H., Fernald,A.A., Le Beau,M.M.,
            Takeuchi,T. and Takeda,J.
  TITLE     Identification and characterization of the gene encoding a second
            proteolipid subunit of human vacuolar H(+)-ATPase (ATP6F)
  JOURNAL   Genomics 50 (2), 222-228 (1998)
   PUBMED   9653649
REFERENCE   9  (bases 1 to 987)
  AUTHORS   Finbow,M.E. and Harrison,M.A.
  TITLE     The vacuolar H+-ATPase: a universal proton pump of eukaryotes
  JOURNAL   Biochem J 324 (Pt 3) (Pt 3), 697-712 (1997)
   PUBMED   9210392
  REMARK    Review article
REFERENCE   10 (bases 1 to 987)
  AUTHORS   Stevens,T.H. and Forgac,M.
  TITLE     Structure, function and regulation of the vacuolar (H+)-ATPase
  JOURNAL   Annu Rev Cell Dev Biol 13, 779-808 (1997)
   PUBMED   9442887
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from D89052.1 and AI439545.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004047.4.
            
            Summary: This gene encodes a portion of the V0 domain of vacuolar
            ATPase (V-ATPase), a multisubunit enzyme that mediates
            acidification of eukaryotic intracellular organelles. Activity of
            this enzyme is necessary for such varied processes as protein
            sorting, zymogen activation, receptor-mediated endocytosis, and
            synaptic vesicle proton gradient generation. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jun
            2014].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163657.209847.1,
                                           SRR1163657.531589.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000472174.7/ ENSP00000431605.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-986               D89052.1           2-987
            987-987             AI439545.1         12-12               c
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p34.1"
     gene            1..987
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /note="ATPase H+ transporting V0 subunit b"
                     /db_xref="GeneID:533"
                     /db_xref="HGNC:HGNC:861"
                     /db_xref="MIM:603717"
     exon            1..148
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..699
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /EC_number="7.1.2.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     V-ATPase 21 kDa proteolipid subunit c''; vacuolar ATP
                     synthase 21 kDa proteolipid subunit; H(+)-transporting
                     two-sector ATPase, subunit F; V-type proton ATPase 21 kDa
                     proteolipid subunit; V-ATPase subunit c''; vacuolar proton
                     pump 21 kDa proteolipid subunit; ATPase, H+ transporting,
                     lysosomal, 21-KD, V0 subunit C-prime, prime; V-ATPase
                     subunit b; ATPase, H+ transporting, lysosomal, subunit F;
                     ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b;
                     vacuolar proton pump 21 kDa proteolipid subunit c'';
                     V-type proton ATPase 21 kDa proteolipid subunit c''"
                     /codon_start=1
                     /product="V-type proton ATPase 21 kDa proteolipid subunit
                     c'' isoform 1"
                     /protein_id="NP_004038.1"
                     /db_xref="CCDS:CCDS505.1"
                     /db_xref="GeneID:533"
                     /db_xref="HGNC:HGNC:861"
                     /db_xref="MIM:603717"
                     /translation="MTGLALLYSGVFVAFWACALAVGVCYTIFDLGFRFDVAWFLTET
                     SPFMWSNLGIGLAISLSVVGAAWGIYITGSSIIGGGVKAPRIKTKNLVSIIFCEAVAI
                     YGIIMAIVISNMAEPFSATDPKAIGHRNYHAGYSMFGAGLTVGLSNLFCGVCVGIVGS
                     GAALADAQNPSLFVKILIVEIFGSAIGLFGVIVAILQTSRVKMGD"
     exon            149..197
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     exon            198..281
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     exon            282..359
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     exon            360..429
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     exon            430..481
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     exon            482..672
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     exon            673..987
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /inference="alignment:Splign:2.1.0"
     regulatory      962..967
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /note="hexamer: AATAAA"
     polyA_site      987
                     /gene="ATP6V0B"
                     /gene_synonym="ATP6F; HATPL; VMA16"
                     /note="major polyA site"
ORIGIN      
        1 agactgcggg acggacggtg gacgctggga cgcgtttgta gctccggccc cgccgttccg
       61 acccccgccg ccgtcgccgc catgacgggg ctagcactgc tctactccgg ggtcttcgtg
      121 gccttctggg cctgcgcgct ggccgtggga gtctgctaca ccatttttga tttgggcttc
      181 cgctttgatg tggcatggtt cctgacggag acttcgccct tcatgtggtc caacctgggc
      241 attggcctag ctatctccct gtctgtggtt ggggcagcct ggggcatcta tattaccggc
      301 tcctccatca ttggtggagg agtgaaggcc cccaggatca agaccaagaa cctggtcagc
      361 atcatcttct gtgaggctgt ggccatctac ggcatcatca tggcaattgt cattagcaac
      421 atggctgagc ctttcagtgc cacagacccc aaggccatcg gccatcggaa ctaccatgca
      481 ggctactcca tgtttggggc tggcctcacc gtaggcctgt ctaacctctt ctgtggagtc
      541 tgcgtgggca tcgtgggcag tggggctgcc ctggccgatg ctcagaaccc cagcctcttt
      601 gtaaagattc tcatcgtgga gatctttggc agcgccattg gcctctttgg ggtcatcgtc
      661 gcaattcttc agacctccag agtgaagatg ggtgactaga tgatatgtgt gggtggggcc
      721 gtgcctcact tttatttatt gctggttttc ctgggacagc tggagctgtg tcccttagcc
      781 tttcagaggc ttggtgttca gggccctccc tgcactcccc tcttgctgcg tgttgatttg
      841 gaggcactgc agtccaggcc gagtcctcag tgcggggagc aggctgctgc tgctgactct
      901 gtgcagctgc gcacctgtgt cccccacctc caccctcaac ccatcttcct agtgtttgtg
      961 aaataaactt ggtatttgtc tgggtca
//
