LOCUS       NM_012463               6507 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens ATPase H+ transporting V0 subunit a2 (ATP6V0A2), mRNA.
ACCESSION   NM_012463
VERSION     NM_012463.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Zhang,Y., Sun,M., Li,N., Zhao,Y., Zhang,F., Shu,J., Liu,Y. and
            Cai,C.
  TITLE     Identification of a novel intronic variant of ATP6V0A2 in a
            Han-Chinese family with cutis laxa
  JOURNAL   Mol Biol Rep 51 (1), 498 (2024)
   PUBMED   38598037
  REMARK    GeneRIF: Identification of a novel intronic variant of ATP6V0A2 in
            a Han-Chinese family with cutis laxa.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Chu,A., Yao,Y., Glibowicka,M., Deber,C.M. and Manolson,M.F.
  TITLE     The Human Mutation K237_V238del in a Putative Lipid Binding Motif
            within the V-ATPase a2 Isoform Suggests a Molecular Mechanism
            Underlying Cutis Laxa
  JOURNAL   Int J Mol Sci 25 (4), 2170 (2024)
   PUBMED   38396846
  REMARK    GeneRIF: The Human Mutation K237_V238del in a Putative Lipid
            Binding Motif within the V-ATPase a2 Isoform Suggests a Molecular
            Mechanism Underlying Cutis Laxa.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6507)
  AUTHORS   Zaman,Q., Iftikhar,A., Rehman,G., Khan,Q., Najumuddin, Jan,A.,
            Khan,J., Anas,M., Laiba, Umair,M., Muthaffar,O.Y.,
            Abdulkareem,A.A., Bibi,F., Naseer,M.I. and Jelani,M.
  TITLE     Two novel homozygous variants of ATP6V0A2 and ALDH18A1 lead to
            autosomal recessive cutis laxa type 2 and 3 in two Pakistani
            families
  JOURNAL   J Gene Med 25 (10), e3522 (2023)
   PUBMED   37119015
  REMARK    GeneRIF: Two novel homozygous variants of ATP6V0A2 and ALDH18A1
            lead to autosomal recessive cutis laxa type 2 and 3 in two
            Pakistani families.
REFERENCE   4  (bases 1 to 6507)
  AUTHORS   Wang,L., Wu,D., Robinson,C.V., Wu,H. and Fu,T.M.
  TITLE     Structures of a Complete Human V-ATPase Reveal Mechanisms of Its
            Assembly
  JOURNAL   Mol Cell 80 (3), 501-511 (2020)
   PUBMED   33065002
REFERENCE   5  (bases 1 to 6507)
  AUTHORS   Vasanthakumar,T. and Rubinstein,J.L.
  TITLE     Structure and Roles of V-type ATPases
  JOURNAL   Trends Biochem Sci 45 (4), 295-307 (2020)
   PUBMED   32001091
  REMARK    Review article
REFERENCE   6  (bases 1 to 6507)
  AUTHORS   Kawasaki-Nishi,S., Nishi,T. and Forgac,M.
  TITLE     Proton translocation driven by ATP hydrolysis in V-ATPases
  JOURNAL   FEBS Lett 545 (1), 76-85 (2003)
   PUBMED   12788495
  REMARK    Review article
REFERENCE   7  (bases 1 to 6507)
  AUTHORS   Nishi,T. and Forgac,M.
  TITLE     The vacuolar (H+)-ATPases--nature's most versatile proton pumps
  JOURNAL   Nat Rev Mol Cell Biol 3 (2), 94-103 (2002)
   PUBMED   11836511
  REMARK    Review article
REFERENCE   8  (bases 1 to 6507)
  AUTHORS   Van Maldergem,L., Dobyns,W. and Kornak,U.
  TITLE     ATP6V0A2-Related Cutis Laxa
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301755
REFERENCE   9  (bases 1 to 6507)
  AUTHORS   Lee,C., Ghoshal,K. and Beaman,K.D.
  TITLE     Cloning of a cDNA for a T cell produced molecule with a putative
            immune regulatory role
  JOURNAL   Mol Immunol 27 (11), 1137-1144 (1990)
   PUBMED   2247090
REFERENCE   10 (bases 1 to 6507)
  AUTHORS   Dautry-Varsat,A.
  TITLE     Receptor-mediated endocytosis: the intracellular journey of
            transferrin and its receptor
  JOURNAL   Biochimie 68 (3), 375-381 (1986)
   PUBMED   2874839
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC068531.1, DA737084.1,
            BI562012.1, AC117503.8 and R51339.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_012463.3.
            
            Summary: The protein encoded by this gene is a subunit of the
            vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is
            present in intracellular vesicles and in the plasma membrane of
            specialized cells, and which is essential for the acidification of
            diverse cellular components. V-ATPase is comprised of a membrane
            peripheral V(1) domain for ATP hydrolysis, and an integral membrane
            V(0) domain for proton translocation. The subunit encoded by this
            gene is a component of the V(0) domain. Mutations in this gene are
            a cause of both cutis laxa type II and wrinkly skin syndrome.
            [provided by RefSeq, Jul 2009].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.270118.1,
                                           SRR1660805.19163.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000330342.8/ ENSP00000332247.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-638               BC068531.1         36-673
            639-684             DA737084.1         217-262
            685-1727            BC068531.1         720-1762
            1728-1821           BI562012.1         532-625
            1822-3025           BC068531.1         1857-3060
            3026-6321           AC117503.8         64940-68235         c
            6322-6507           R51339.1           9-194               c
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.31"
     gene            1..6507
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /note="ATPase H+ transporting V0 subunit a2"
                     /db_xref="GeneID:23545"
                     /db_xref="HGNC:HGNC:18481"
                     /db_xref="MIM:611716"
     exon            1..330
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..2784
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /EC_number="7.1.2.1"
                     /note="v-type proton ATPase 116 kDa subunit a; ATPase, H+
                     transporting, lysosomal V0 subunit a2; vacuolar proton
                     translocating ATPase 116 kDa subunit a; A2V-ATPase;
                     regeneration and tolerance factor; lysosomal
                     H(+)-transporting ATPase V0 subunit a2; V-ATPase 116 kDa
                     subunit a2; V-type proton ATPase 116 kDa subunit a2;
                     V-ATPase 116 kDa subunit a 2; lysosomal H(+)-transporting
                     ATPase V0 subunit a 2; V-ATPase subunit a2"
                     /codon_start=1
                     /product="V-type proton ATPase 116 kDa subunit a 2"
                     /protein_id="NP_036595.2"
                     /db_xref="CCDS:CCDS9254.1"
                     /db_xref="GeneID:23545"
                     /db_xref="HGNC:HGNC:18481"
                     /db_xref="MIM:611716"
                     /translation="MGSLFRSETMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNV
                     SSFQRKFVGEVKRCEELERILVYLVQEINRADIPLPEGEASPPAPPLKQVLEMQEQLQ
                     KLEVELREVTKNKEKLRKNLLELIEYTHMLRVTKTFVKRNVEFEPTYEEFPSLESDSL
                     LDYSCMQRLGAKLGFVSGLINQGKVEAFEKMLWRVCKGYTIVSYAELDESLEDPETGE
                     VIKWYVFLISFWGEQIGHKVKKICDCYHCHVYPYPNTAEERREIQEGLNTRIQDLYTV
                     LHKTEDYLRQVLCKAAESVYSRVIQVKKMKAIYHMLNMCSFDVTNKCLIAEVWCPEAD
                     LQDLRRALEEGSRESGATIPSFMNIIPTKETPPTRIRTNKFTEGFQNIVDAYGVGSYR
                     EVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLNQSQEIMRMFFNG
                     RYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHPPAEHKKMVLWNDSV
                     VRHNSILQLDPSIPGVFRGPYPLGIDPIWNLATNRLTFLNSFKMKMSVILGIIHMTFG
                     VILGIFNHLHFRKKFNIYLVSIPELLFMLCIFGYLIFMIFYKWLVFSAETSRVAPSIL
                     IEFINMFLFPASKTSGLYTGQEYVQRVLLVVTALSVPVLFLGKPLFLLWLHNGRSCFG
                     VNRSGYTLIRKDSEEEVSLLGSQDIEEGNHQVEDGCREMACEEFNFGEILMTQVIHSI
                     EYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVIALFA
                     VLTIFILLIMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFNNDDSVA
                     "
     exon            331..409
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            410..507
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            508..645
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            646..734
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            735..861
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            862..944
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1038
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1039..1251
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1402
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1539
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1727
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1728..1818
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1937
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2148
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2149..2268
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2388
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2506
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2678
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..6507
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3007..3012
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /note="hexamer: AATATA"
     polyA_site      3025
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /note="major polyA site"
     regulatory      5430..5435
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /note="hexamer: AGTAAA"
     polyA_site      5451
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
     regulatory      6488..6493
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /note="hexamer: AATATA"
     polyA_site      6507
                     /gene="ATP6V0A2"
                     /gene_synonym="A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D;
                     J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS"
                     /note="major polyA site"
ORIGIN      
        1 gggacctcgc ggactgctgt ggcggcagct ggagcggcgg ccgcggtggc agaaccgggg
       61 gcggccgctg cagtctggag ccccatagtg cggggccgcg gccaggccac aggaagagct
      121 cgaggcccgg gccgcaccgg ctgagtgtgc gggcccgcgc ggctcggagc cgccgccgcc
      181 catcgagccc ctccgggcgc gggtcggccc gccatggggt ccctgttccg gagcgagacc
      241 atgtgcctgg cgcagctctt cctgcagtcg ggcacggcct acgagtgcct cagcgccctg
      301 ggcgagaaag gcctggtcca gttccgagac ctcaaccaga acgtaagttc tttccaaaga
      361 aaatttgttg gtgaggtgaa gaggtgtgaa gagctagagc gaatattggt gtatttggta
      421 caggaaatta atagagctga tattcccctt cctgaaggag aggccagccc tcctgcgcca
      481 cccctgaaac aggttctaga aatgcaggag cagttgcaga agctcgaggt tgaactgaga
      541 gaagtcacta agaacaagga gaaactgagg aaaaacttgc tggaactgat agagtacact
      601 cacatgctga gagtgacaaa gacctttgtg aaacgcaatg ttgagtttga acccacttat
      661 gaagaattcc cttccttaga gagtgattct ttgttggatt acagctgtat gcagaggctg
      721 ggagcaaaac tgggatttgt gtctggccta attaaccaag gaaaagtgga agcatttgaa
      781 aaaatgttgt ggagagtctg caaagggtac accatcgtgt cctatgcaga actggatgaa
      841 tcccttgaag accctgaaac aggggaagtc ataaaatggt atgtcttttt aatatccttt
      901 tggggagagc agattggcca caaggttaag aagatatgtg attgctacca ctgccacgtg
      961 tacccctatc caaacacagc cgaggagcgg agggagatcc aggaggggct gaacacccgc
     1021 atccaggatc tctacactgt actgcacaaa accgaggact atttgaggca agtgctatgt
     1081 aaagccgccg agtctgtcta cagccgtgtg atccaggtga agaaaatgaa ggccatctat
     1141 cacatgctga acatgtgcag ctttgacgtg accaacaagt gcctcattgc tgaggtctgg
     1201 tgtcccgagg cggatctgca ggacctgcgc cgggcactgg aggagggctc gagagagagt
     1261 ggtgctacaa tcccctcatt catgaatata atccccacaa aagaaacacc ccccactcgg
     1321 atccgcacca acaaattcac cgagggattt cagaacatcg tggatgctta tggagtcgga
     1381 agctacagag aagtcaatcc agctctcttt accatcatca ccttcccgtt tttatttgct
     1441 gtgatgtttg gagacttcgg acatggcttt gtgatgtttt tatttgccct cttgttggtg
     1501 ttaaatgaaa atcatcccag actaaatcag tcacaagaga tcatgaggat gttttttaat
     1561 ggccggtaca tcctcctgct gatggggctg ttctcagtgt acactggcct catctacaac
     1621 gactgctttt caaagtcagt caacctgttc ggctctgggt ggaacgtgtc ggccatgtac
     1681 agctccagcc acccacccgc agagcataag aagatggtgc tttggaatga cagcgtcgtt
     1741 agacacaaca gcattttgca gctggatcca agcattcctg gagtgttccg aggcccttat
     1801 ccccttggca ttgatcctat ttggaacttg gccacaaatc gcctcacttt tctaaactct
     1861 ttcaaaatga aaatgtccgt gattttagga atcattcata tgacttttgg agtcattctg
     1921 ggaatattta accacttgca cttcaggaag aagttcaaca tttacctggt ttccatcccg
     1981 gaacttctct tcatgctctg tatctttgga taccttatat ttatgatttt ctacaagtgg
     2041 ctggtttttt cagcagaaac ctccagagtt gctcccagca ttctgattga atttattaac
     2101 atgtttttat tcccagccag taaaacaagt ggcctttaca cagggcagga gtatgtccag
     2161 agagtgctgc tggttgtcac agcattgtct gtccctgtcc tcttcttggg aaagccactg
     2221 tttttgttgt ggcttcacaa tgggcgtagt tgcttcgggg tgaaccggag tggctacaca
     2281 cttataagga aagatagtga ggaagaagtt tcattgctgg gaagccaaga tatagaagag
     2341 ggaaatcacc aggtggaaga tggatgtaga gaaatggcgt gtgaagagtt taattttgga
     2401 gaaatattaa tgacccaagt aatccattcc atcgagtact gtctgggatg catctccaac
     2461 accgcctcct acctgaggct ctgggcgctt agcctggctc acgcacagtt gtctgatgtc
     2521 ctgtgggcca tgctgatgcg cgtgggcctc cgcgttgaca ccacctatgg cgtcttgcta
     2581 ctgctcccgg ttatcgcgct ctttgcagtt ttgaccattt tcatccttct gatcatggaa
     2641 gggctttctg cgtttcttca cgccatacgc ctccactggg tagaatttca gaacaaattc
     2701 tacgttggtg caggcaccaa atttgttcct ttctcattca gtctactttc atcaaagttc
     2761 aataacgacg acagtgtggc atgatcatat tgctgtaacc aacaagcttt cagatttatg
     2821 gagaatgacc atgttataga ctttcactta tgtcagattt atgataggaa aaattccatc
     2881 ttcattactg ccttatgaca tagccaaata attctgtaag atatacctct tcctcatatg
     2941 ttaaatattt tgtaaagttt accaatttga gatataaaaa tttcttttgg ttttttatga
     3001 tgagcaaata taagttaatg ccaaacgtta tgttaaagtt attttttcaa atacaggatt
     3061 tggggagaga agccaatttt gcatggctag ttgaaaatgg tattagatac ttatttcctt
     3121 taaactttat ttaaaacaaa caacagagtt aatctgtcac ctgaagttgc cagataatat
     3181 tttccaacag ctgaagtagg gatagttttt taaaaaattt aatgatgggt aatcaaaaca
     3241 ttcacattta ttctgctgga tttaaaaata tgtacttttt tttttttttt gagatgaagt
     3301 cttgctgtgt cacgtaggct ggagtgcaat ggcacgatcg tgtctcattg caacttccgc
     3361 ctcctggatt caggtgattc tcctgtctca gcctcccgag taactgggat tacaggcatg
     3421 cgccaccgtg accagctaat ttttttgtat ttttgtagag atggggtttc accatgttgg
     3481 ccaggctggt ctcgaacttc tgacctcaag tgatccacct gcctcggcct ccctaagtgc
     3541 tgggattaca ggcgtgagcc accatgcctg gccaaaatat gcaaatattt tttatcctgt
     3601 gctacctaga attacaagta gaggattgtt tttacaattt tgtcttgttt tagtaattgg
     3661 ggacaatggg ataaaatgac atacaacaaa tggttatccc acttgtatat ttttttaaag
     3721 actcttatcc tgttaccctt acataaaacc agttatgata attgtatgtt tggaagataa
     3781 attgtatgct gagccttaaa acccagtgtg actgtagaag atataatgcg gacatgattt
     3841 gaatttagtg acagcttggc tgatgtcact gaaccagaag ggtttatact gagtgaagga
     3901 aaggtaaaaa gtagtatttt gtatattttt gtaacaaaat ataaatgaag atattttacc
     3961 atgaagagat tgcacttatc cttgagaaca gtctaaatat aaatgaagat attttaccat
     4021 gaagagattg cacttatcct tgagaacagt ctaatgatac tttttttact ttcagactgt
     4081 ttctagctaa agttctgata aaagtattga gttaatttaa actttcaaac actgtttaga
     4141 tcagttgaaa actaattctt aatagccatt gttttataat ctttagttta aattcatctg
     4201 tttgacattt ggttttttgt gaacttgagt cagtcctatt gaacattttg acctatattc
     4261 ttgcaaatta tgttaatttt tcatatttta gtttgatttt agacaattat tttcaaaaag
     4321 aaacctgact ttttttcacc ttcttgacat tgtgagtgat acacgttaaa aggctatttg
     4381 cccgcatcct cctctatcct gtttcgaggt agagagcttg gcacttctgt tacaagctga
     4441 gagtctggag gtgctgagtt taacgtggct gtgcagctga tttcaaggca tgagctgaac
     4501 agcgttgaca cagccgtgac ttacaattaa accctttcaa ttacagtaca gattctgtgt
     4561 gctacttgat gagatgttac ccaaggccag tttggactta ctgctttttg tccctcatat
     4621 taaattatca aatgatgccg atgaaatgct tgaaaatgat tctgctctca aagttatgct
     4681 tccctagaaa ggtgtcatgt cgggcacttg tgttgggtga ctatgacatc ccagcagtgc
     4741 tggtcaggtc tcagagttgc tttcacctag agctgatgct tccacaaggg gacatttgtg
     4801 ttactttgct ccaggggtct gtcataaaaa caatggtaac tacttcatcc ctaggaagcc
     4861 caagacttca gggcactgtt agctgattgg taaataaggg acacgatatt cacgggaatt
     4921 gtttactgct ccccactagt aattctcatg gggccacttt tttaactctt tctaattaac
     4981 acctcttctc agcatagagc agaggatgca gtcatttctc tgttgaaatc tatgtagtat
     5041 ttatgtagaa tgtcattata tgaaaagcga attcagaatc ctaactctgg gaaagcgctc
     5101 tctggaaatg tagtttgata atagtgtctt ataggggcca cgggaatttg tttctctata
     5161 aagcgatggg ctccagtgtc atgttaccag agttatcgct cagttttgtt tgttaaggct
     5221 tttgctagaa cctattatgt atccagtttt taaaacatac ttcattccct tgatagcaga
     5281 gatccagagc acagatggca agttgtgtgg atgatactga atatgtttat tttcctaaag
     5341 ataaatgtca cgacacgaca tttctcagtt tttatttaga tgtttattac catgtgttaa
     5401 cagaatctta aaacccaagg gatttcttca gtaaactaga ctttgattca acctgctggg
     5461 ctggagactg taattgccgt ggtcagttaa ggagtgcaaa gggtgttctg tctgatagga
     5521 tctgggtgag ccctgcggac tttccagcag cgaaaggact tggaactcct ctttcgctga
     5581 atgctgctct gtttctggaa tggatatttt ataacaacat tctggtgtca gcatcccctc
     5641 agcagttgtt tatttaaaaa tttttttatt ttttttgaga cagtgtctca ctctgtcacc
     5701 taggctgaag tgcagtggtg cagtctcggc tcactgcaac ctctgcctcc tgggtttaag
     5761 caattctcct gccctagcct cccgagtagc tgggattaca ggcgcctgcc acagtgcccg
     5821 gctaattttt gtatttttag tagagacagg gtttcaccat gttggccagg ctggtctcaa
     5881 actcctgacc tcaggtgatc tacccacctt ggcctcccaa agtgctggga ttacaggcgt
     5941 gagccaccgc gcctggccct ctgcagttgt ttaataaggc acagaatacc tgtagcatag
     6001 gtcagcctta cgatgtccat gaattacata ttcagacgtt ttagagcctg atacattttg
     6061 gaaaagaaaa acaacttcta cacctattct acagtccgca tttaaaacaa taaattcctc
     6121 tattaaaaac gtaaagccgg gtttgcttgc gtgccacagg gaatatatcc aggaaggtta
     6181 ttatgaagct gtcaaatcaa gatgatggga ataaggcagt ttgaacgaac agtcttccca
     6241 cagtcaggcc atttttgctg atttggttta gaattttcag aaatacttag tacactccac
     6301 ctgttctttg atgggaatat ctaagaaggc taggtaggtt cttagggtta gcctgagtca
     6361 tctaggggct caactccttg tgaggggaaa tgacagtgaa caagttagta ctttgctcca
     6421 caaatgcatg aaaggacaaa tttgcatctt ctatcagtat ttaacttcct tttgctaatg
     6481 acaaataaat atatctatat ttttaaa
//
