LOCUS       NM_001003703             526 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens ATP synthase peripheral stalk subunit F6 (ATP5PF),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001003703
VERSION     NM_001003703.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 526)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 526)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 526)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   5  (bases 1 to 526)
  AUTHORS   Bai,B., Xie,B., Pan,Z., Shan,L., Zhao,J. and Zhu,H.
  TITLE     Identification of candidate genes and long non-coding RNAs
            associated with the effect of ATP5J in colorectal cancer
  JOURNAL   Int J Oncol 52 (4), 1129-1138 (2018)
   PUBMED   29484395
  REMARK    GeneRIF: AKT2 and XIST expression was identified as a potential
            biomarker participating in the effect of ATP5J in colorectal cancer
REFERENCE   6  (bases 1 to 526)
  AUTHORS   Adams,M.D., Kerlavage,A.R., Fleischmann,R.D., Fuldner,R.A.,
            Bult,C.J., Lee,N.H., Kirkness,E.F., Weinstock,K.G., Gocayne,J.D.,
            White,O. et al.
  TITLE     Initial assessment of human gene diversity and expression patterns
            based upon 83 million nucleotides of cDNA sequence
  JOURNAL   Nature 377 (6547 Suppl), 3-174 (1995)
   PUBMED   7566098
REFERENCE   7  (bases 1 to 526)
  AUTHORS   Hochstrasser,D.F., Frutiger,S., Paquet,N., Bairoch,A., Ravier,F.,
            Pasquali,C., Sanchez,J.C., Tissot,J.D., Bjellqvist,B., Vargas,R. et
            al.
  TITLE     Human liver protein map: a reference database established by
            microsequencing and gel comparison
  JOURNAL   Electrophoresis 13 (12), 992-1001 (1992)
   PUBMED   1286669
REFERENCE   8  (bases 1 to 526)
  AUTHORS   Higuti,T., Tsurumi,C., Kawamura,Y., Tsujita,H., Osaka,F.,
            Yoshihara,Y., Tani,I., Tanaka,K. and Ichihara,A.
  TITLE     Molecular cloning of cDNA for the import precursor of human
            coupling factor 6 of H(+)-ATP synthase in mitochondria
  JOURNAL   Biochem Biophys Res Commun 178 (2), 793-799 (1991)
   PUBMED   1830479
REFERENCE   9  (bases 1 to 526)
  AUTHORS   Javed,A.A., Ogata,K. and Sanadi,D.R.
  TITLE     Human mitochondrial ATP synthase: cloning cDNA for the
            nuclear-encoded precursor of coupling factor 6
  JOURNAL   Gene 97 (2), 307-310 (1991)
   PUBMED   1825642
REFERENCE   10 (bases 1 to 526)
  AUTHORS   Boyer,P.D., Cross,R.L. and Momsen,W.
  TITLE     A new concept for energy coupling in oxidative phosphorylation
            based on a molecular explanation of the oxygen exchange reactions
  JOURNAL   Proc Natl Acad Sci U S A 70 (10), 2837-2839 (1973)
   PUBMED   4517936
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ268216.1, BC001178.1,
            BC066310.1 and AI085837.1.
            
            On Aug 11, 2020 this sequence version replaced NM_001003703.1.
            
            Summary: Mitochondrial ATP synthase catalyzes ATP synthesis,
            utilizing an electrochemical gradient of protons across the inner
            membrane during oxidative phosphorylation. It is composed of two
            linked multi-subunit complexes: the soluble catalytic core, F1, and
            the membrane-spanning component, Fo, which comprises the proton
            channel. The F1 complex consists of 5 different subunits (alpha,
            beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3
            beta, and a single representative of the other 3. The Fo complex
            has nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene
            encodes the F6 subunit of the Fo complex. The F6 subunit is
            required for F1 and Fo interactions. Alternatively spliced
            transcript variants encoding different isoforms have been
            identified for this gene. This gene has 1 or more pseudogenes.
            [provided by RefSeq, Feb 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2, 3, 4, 6 and 7 encode the same isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM772791.1, DA684306.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2148093, SAMEA2148874
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000284971.8/
                                                  ENSP00000284971.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                BQ268216.1         75-126
            53-481              BC001178.1         686-1114
            482-524             BC066310.1         548-590
            525-526             AI085837.1         28-29               c
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="21"
                     /map="21q21.3"
     gene            1..526
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /note="ATP synthase peripheral stalk subunit F6"
                     /db_xref="GeneID:522"
                     /db_xref="HGNC:HGNC:847"
                     /db_xref="MIM:603152"
     exon            1..52
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /inference="alignment:Splign:2.1.0"
     exon            53..223
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..386
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /EC_number="3.6.1.14"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; ATP synthase, H+ transporting, mitochondrial F0
                     complex, subunit F6; coupling factor 6; mitochondrial ATP
                     synthase, subunit F6; mitochondrial ATPase coupling factor
                     6; mitochondrial ATP synthase, coupling factor 6; ATP
                     synthase-coupling factor 6, mitochondrial;
                     proliferation-inducing protein 36; ATPase subunit F6; ATP
                     synthase, H+ transporting, mitochondrial Fo complex
                     subunit F6; ATP synthase subunit h"
                     /codon_start=1
                     /product="ATP synthase-coupling factor 6, mitochondrial
                     isoform a precursor"
                     /protein_id="NP_001003703.1"
                     /db_xref="CCDS:CCDS13574.1"
                     /db_xref="GeneID:522"
                     /db_xref="HGNC:HGNC:847"
                     /db_xref="MIM:603152"
                     /translation="MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFV
                     DKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEV
                     IEKPQA"
     exon            224..348
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /inference="alignment:Splign:2.1.0"
     exon            349..526
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      454..459
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /note="hexamer: AATAAA"
     polyA_site      481
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /note="major polyA site"
     regulatory      504..509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /note="hexamer: AATAAA"
     polyA_site      526
                     /gene="ATP5PF"
                     /gene_synonym="ATP5; ATP5A; ATP5J; ATPM; CF6; F6"
                     /note="major polyA site"
ORIGIN      
        1 agagcggagg tggtggcggc ggaggctttg gcagctcggg actgagtgca agaatcagca
       61 tgattcttca gaggctcttc aggttctcct ctgtcattcg gtcagccgtc tcagtccatt
      121 tgcggaggaa cattggtgtt acagcagtgg catttaataa ggaacttgat cctatacaga
      181 aactctttgt ggacaagatt agagaataca aatctaagcg acagacatct ggaggacctg
      241 ttgatgctag ttcagagtat cagcaagagc tggagaggga gctttttaag ctcaagcaaa
      301 tgtttggtaa tgcagacatg aatacatttc ccaccttcaa atttgaagat cccaaatttg
      361 aagtcatcga aaaaccccag gcctgaagaa ataaagtaaa attaatctgg taatttgtca
      421 cggattagtt gtacaactag ttagaagttt cagaataaac atgcatttca taactgtcaa
      481 atgttctttt aattctgagt ccaaataaat tatttggtga tgttga
//
