LOCUS       NM_001684               8697 bp    mRNA    linear   PRI 10-FEB-2025
DEFINITION  Homo sapiens ATPase plasma membrane Ca2+ transporting 4 (ATP2B4),
            transcript variant 2, mRNA.
ACCESSION   NM_001684
VERSION     NM_001684.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Miya,V., Kumar,C., Breed,A.A., Idicula-Thomas,S. and Pathak,B.R.
  TITLE     Mammalian cysteine-rich secretory proteins interact with plasma
            membrane Ca2+ exporter PMCA4b
  JOURNAL   Andrology 12 (5), 1096-1110 (2024)
   PUBMED   37882330
  REMARK    GeneRIF: Mammalian cysteine-rich secretory proteins interact with
            plasma membrane Ca[2+] exporter PMCA4b.
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Yoshie,S., Kuriyama,M., Maekawa,M., Xu,W., Niidome,T., Futaki,S.
            and Hirose,H.
  TITLE     ATP2B4 is an essential gene for epidermal growth factor-induced
            macropinocytosis in A431 cells
  JOURNAL   Genes Cells 29 (6), 512-520 (2024)
   PUBMED   38597132
  REMARK    GeneRIF: ATP2B4 is an essential gene for epidermal growth
            factor-induced macropinocytosis in A431 cells.
REFERENCE   3  (bases 1 to 8697)
  AUTHORS   Thiam,A., Nisar,S., Adjemout,M., Gallardo,F., Ka,O., Mbengue,B.,
            Diop,G., Dieye,A., Marquet,S. and Rihet,P.
  TITLE     ATP2B4 regulatory genetic variants are associated with mild malaria
  JOURNAL   Malar J 22 (1), 68 (2023)
   PUBMED   36849945
  REMARK    GeneRIF: ATP2B4 regulatory genetic variants are associated with
            mild malaria.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8697)
  AUTHORS   Joof,F., Hartmann,E., Jarvis,A., Colley,A., Cross,J.H., Avril,M.,
            Prentice,A.M. and Cerami,C.
  TITLE     Genetic variations in human ATP2B4 gene alter Plasmodium falciparum
            in vitro growth in RBCs from Gambian adults
  JOURNAL   Malar J 22 (1), 5 (2023)
   PUBMED   36604655
  REMARK    GeneRIF: Genetic variations in human ATP2B4 gene alter Plasmodium
            falciparum in vitro growth in RBCs from Gambian adults.
            Erratum:[Malar J. 2023 May 25;22(1):164. doi:
            10.1186/s12936-023-04565-8.. PMID: 37231516]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8697)
  AUTHORS   Ambartsumyan,L.
  TITLE     Megacystis-Microcolon-Intestinal Hypoperistalsis Syndrome Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   31070878
REFERENCE   6  (bases 1 to 8697)
  AUTHORS   Hedera,P.
  TITLE     Hereditary Spastic Paraplegia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301682
REFERENCE   7  (bases 1 to 8697)
  AUTHORS   Heim,R., Hug,M., Iwata,T., Strehler,E.E. and Carafoli,E.
  TITLE     Microdiversity of human-plasma-membrane calcium-pump isoform 2
            generated by alternative RNA splicing in the N-terminal coding
            region
  JOURNAL   Eur J Biochem 205 (1), 333-340 (1992)
   PUBMED   1313367
REFERENCE   8  (bases 1 to 8697)
  AUTHORS   Brandt,P., Neve,R.L., Kammesheidt,A., Rhoads,R.E. and Vanaman,T.C.
  TITLE     Analysis of the tissue-specific distribution of mRNAs encoding the
            plasma membrane calcium-pumping ATPases and characterization of an
            alternately spliced form of PMCA4 at the cDNA and genomic levels
  JOURNAL   J Biol Chem 267 (7), 4376-4385 (1992)
   PUBMED   1531651
REFERENCE   9  (bases 1 to 8697)
  AUTHORS   Olson,S., Wang,M.G., Carafoli,E., Strehler,E.E. and McBride,O.W.
  TITLE     Localization of two genes encoding plasma membrane
            Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and
            12q21-23
  JOURNAL   Genomics 9 (4), 629-641 (1991)
   PUBMED   1674727
REFERENCE   10 (bases 1 to 8697)
  AUTHORS   Strehler,E.E., James,P., Fischer,R., Heim,R., Vorherr,T.,
            Filoteo,A.G., Penniston,J.T. and Carafoli,E.
  TITLE     Peptide sequence analysis and molecular cloning reveal two calcium
            pump isoforms in the human erythrocyte membrane
  JOURNAL   J Biol Chem 265 (5), 2835-2842 (1990)
   PUBMED   2137451
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL706212.1, DN997129.1,
            M25874.1, BF985985.1, AC114402.2 and AW888226.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001684.4.
            
            Summary: The protein encoded by this gene belongs to the family of
            P-type primary ion transport ATPases characterized by the formation
            of an aspartyl phosphate intermediate during the reaction cycle.
            These enzymes remove bivalent calcium ions from eukaryotic cells
            against very large concentration gradients and play a critical role
            in intracellular calcium homeostasis. The mammalian plasma membrane
            calcium ATPase isoforms are encoded by at least four separate genes
            and the diversity of these enzymes is further increased by
            alternative splicing of transcripts. The expression of different
            isoforms and splice variants is regulated in a developmental,
            tissue- and cell type-specific manner, suggesting that these pumps
            are functionally adapted to the physiological needs of particular
            cells and tissues. This gene encodes the plasma membrane calcium
            ATPase isoform 4. Alternatively spliced transcript variants
            encoding different isoforms have been identified. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2) lacks an alternate coding exon
            compared to variant 1, that causes a frameshift. The resulting
            isoform (4b) has a longer and distinct C-terminus compared to
            isoform 4a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX537444.1, CR749393.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357681.10/ ENSP00000350310.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-445               AL706212.1         33-477
            446-981             DN997129.1         163-698
            982-1169            M25874.1           527-714
            1170-1220           BF985985.1         211-261             c
            1221-4521           M25874.1           766-4066
            4522-8681           AC114402.2         48977-53136
            8682-8697           AW888226.1         2887-2902
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..8697
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /note="ATPase plasma membrane Ca2+ transporting 4"
                     /db_xref="GeneID:493"
                     /db_xref="HGNC:HGNC:817"
                     /db_xref="MIM:108732"
     exon            1..388
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            389..1045
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     CDS             853..4470
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /EC_number="7.2.2.10"
                     /note="isoform 4b is encoded by transcript variant 2;
                     sarcolemmal calcium pump; plasma membrane
                     calcium-transporting ATPase 4; ATPase, Ca++ transporting,
                     plasma membrane 4; matrix-remodeling-associated protein 1"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 4
                     isoform 4b"
                     /protein_id="NP_001675.3"
                     /db_xref="CCDS:CCDS1440.1"
                     /db_xref="GeneID:493"
                     /db_xref="HGNC:HGNC:817"
                     /db_xref="MIM:108732"
                     /translation="MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQI
                     NVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQ
                     DVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVI
                     IVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGD
                     LLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGV
                     NSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDN
                     EEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRR
                     PWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHL
                     DACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGI
                     SINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVY
                     TFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVR
                     TVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAI
                     AKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQ
                     EKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADV
                     GFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVI
                     VAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISR
                     TMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQL
                     FNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWL
                     WCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRR
                     GQILWFRGLNRIQTQIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTP
                     LLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETS
                     V"
     exon            1046..1243
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1501
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1627
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..1753
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            1754..1789
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1951
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2166
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2409
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2651
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2883
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            2884..3063
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3151
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3258
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3259..3450
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3451..3664
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3665..3876
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3877..3984
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            3985..4161
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     exon            4162..8697
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8673..8678
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /note="hexamer: AATAAA"
     polyA_site      8697
                     /gene="ATP2B4"
                     /gene_synonym="ATP2B2; MXRA1; PMCA4; PMCA4b; PMCA4x"
                     /note="major polyA site"
ORIGIN      
        1 gagttagcta gagctgttgc catgacaagc attttcttaa gaaagctctg ctgttgagat
       61 cgtccaaagc tgggggctgg gagggggggc acgaacttcg tggggcacca tccctccgaa
      121 gagagcaaga tcagagacac ccgcccagct ctcctgcacc ctttttcttt caggaacctg
      181 agaggtcctt cccattggag gggcaggggg aggagaccca gcccactccc cggccttagc
      241 cagaaaggca agagagcaaa gccagcgtct ctagcttggt tgccacactg gggagactgg
      301 tgggtggtca ccccacccta ccctcatcca tggaaacccg gagggagagt cccgcctgaa
      361 gaaacctgga tctttgctag aaagagagag atccagttgt catcggtatc caggaagctc
      421 tcctcttcct cctcctgacg tctactacta cagttgctgg ttgttgctaa ggttgctgcc
      481 atggtaacat gcacatcctg tttacacctt catctgggca agttggtcta agctaggaac
      541 ctacctaccc tggacaacta ctatcatcac cacctgggga caccaatcat cgtgacacgg
      601 agtccacctt ccactcagtt cccccatcct cttcctcctc tcgctgccag acttcatacg
      661 gaagaaagga tctagacttc ggacggctac tcgggagctt attgcacaag atatattcaa
      721 tctattccct cactgggccc ccagagaagc aagaagtagg aagaagttga gacagggagg
      781 caggagacac tggtcagttg aagggaaacg ctacatcttc tctggttgag gggcttggta
      841 acagcaggca aaatgacgaa cccatcagac cgtgtcttgc ctgccaactc gatggccgag
      901 agccgtgaag gggactttgg ctgcacagta atggaactga ggaagctcat ggagctgcgt
      961 tcaagggatg cactgaccca gattaatgtc cactatggag gtgtacagaa tctctgcagt
     1021 agactgaaaa cctcccctgt ggaaggtctg tctgggaacc ctgcagatct ggagaaacgt
     1081 aggcaggtgt ttggacacaa cgtgatcccc cccaaaaagc ccaagacttt cttagaatta
     1141 gtgtgggaag ctcttcaaga tgtcacgctt atcatcctgg agattgcagc catcatctcc
     1201 ctggtcctgt ccttttatcg ccctgctggt gaagaaaatg aactgtgtgg tcaagtcgca
     1261 actaccccag aagatgaaaa tgaggcacaa gctggctgga ttgagggggc agccatcctt
     1321 ttctcagtga tcatcgtggt gttagtgact gcctttaatg attggagcaa agagaagcaa
     1381 ttccgggggc tgcagtgccg cattgaacag gagcaaaagt tctccatcat ccgaaacggt
     1441 caactcatcc agctccctgt ggctgagatt gtggttggtg atattgccca agtcaaatac
     1501 ggtgatctgc tgcctgcaga tggaatcctg atccaaggga atgatctgaa gattgatgag
     1561 agctctctga caggggaatc tgaccatgtc aagaagtccc tggacaaaga ccccatgttg
     1621 ctctcaggga cccatgtcat ggaaggttct ggccggatgg tggtgacagc tgttggtgtc
     1681 aactctcaga ctggaatcat ccttactctc ttgggggtca atgaggatga cgaaggggag
     1741 aaaaagaaga aaggtaaaaa acaaggagtc cctgaaaatc gcaacaaagc aaagacccaa
     1801 gacggagtgg ccctggaaat ccagccactc aacagccagg agggaatcga caatgaggaa
     1861 aaggacaaga aggcagtcaa ggtgcctaaa aaggagaagt cagtgctgca gggcaagctg
     1921 actcgcctgg ctgttcagat tgggaaagcc ggtctgctca tgtctgctct cacggttttc
     1981 atcctgattc tatactttgt gattgacaac tttgtgataa atcgcagacc atggctccct
     2041 gagtgtactc ccatctacat ccagtacttt gtcaagttct tcatcatcgg catcactgta
     2101 ctggtggtgg ctgtgccaga ggggctgcct ctggctgtca ccatctcact ggcctactct
     2161 gtgaagaaaa tgatgaaaga caataaccta gtacggcact tggatgcttg tgagaccatg
     2221 ggcaacgcca ccgccatctg ctctgataag acaggcacgt tgaccatgaa ccgcatgact
     2281 gtggtacaag cttatattgg gggcatccat taccgtcaaa tcccaagccc tgatgtcttc
     2341 ctgcccaaag tcctggacct cattgtcaat ggcatttcta tcaacagtgc ttatacctcc
     2401 aagattctgc ctccagagaa ggagggaggc ctgcctcggc aggtgggcaa caagaccgag
     2461 tgtgctctgc taggctttgt cacagatctg aagcaggatt atcaggctgt gcgtaatgaa
     2521 gtgcccgagg agaagctcta caaggtgtac acctttaact cagtgcgcaa gtcaatgagc
     2581 accgtcatca ggaatcccaa cggtggcttc cgtatgtaca gcaagggcgc ctctgagatc
     2641 atcttgcgca agtgtaatcg aatcctggac cggaaagggg aagcagtgcc attcaagaat
     2701 aaagacagag atgatatggt acgcactgtc atcgagccca tggcctgtga tggactccgg
     2761 actatctgca tagcttaccg ggacttcgat gacacagagc cctcttggga caatgagaat
     2821 gagatcctca ccgaactgac ctgtatcgcg gtggtgggca ttgaggaccc tgtgcgccca
     2881 gaggtgccag atgctattgc caaatgcaaa caagctggca ttactgtcag aatggtgaca
     2941 ggtgacaaca tcaacacagc ccgggccatt gccaccaaat gtggcattct gacacctggg
     3001 gatgacttcc tgtgcttaga aggcaaagaa ttcaaccggc tcatccgcaa cgagaaaggc
     3061 gaggtagagc aagaaaagct ggacaagatc tggcctaagc ttcgggtcct ggcgcgatct
     3121 tctcccactg acaagcacac cctggtgaaa ggcataattg acagcactgt tggggaacac
     3181 cggcaggtcg tggctgtcac tggtgatggc acaaatgacg ggcctgctct gaagaaagcg
     3241 gatgttggtt ttgccatggg catcgcaggc acagatgtag caaaggaggc ttcagacatc
     3301 atcctaacag atgacaactt caccagcatt gtgaaggcag tgatgtgggg acgaaatgtc
     3361 tatgacagca tctccaagtt cctgcagttc cagctcactg tcaatgtggt ggccgtgatt
     3421 gtagccttca ctggagcctg tatcactcag gattccccat tgaaagctgt gcagatgttg
     3481 tgggttaatc tgatcatgga cacttttgct tcattggccc tggccacaga gccccctacg
     3541 gaatctctgt tgaagcggcg cccctatggc cgaaataagc ctctgatctc acgcactatg
     3601 atgaagaaca tcttgggcca tgcattctat cagctcattg tcatctttat ccttgtcttt
     3661 gcgggtgaga aattctttga tattgatagt gggaggaagg cacctctaca ttcaccaccc
     3721 agccagcact ataccattgt ttttaacacc ttcgtgctga tgcagctctt caatgaaatc
     3781 aactcccgaa agatccatgg agagaagaac gtcttttcag gcatctaccg caacattatc
     3841 ttctgctctg tagtcttggg cacattcatc tgccagattt tcatcgtgga atttgggggt
     3901 aaacccttca gttgtacaag cctcagcctg tctcagtggc tgtggtgtct cttcattggg
     3961 attggagaac ttctgtgggg ccagttcatc tccgcaatac ctacccgatc cctgaagttc
     4021 ctgaaggagg ctgggcatgg caccaccaaa gaggagatca ccaaggatgc cgagggactg
     4081 gatgagattg accatgctga gatggagctg cgccgaggcc agatcctctg gttccggggc
     4141 ctgaaccgta tccagactca gatcaaagtg gtcaaagcgt tccatagttc cctccacgaa
     4201 agcattcaga aaccctacaa ccaaaagtcc atccacagct tcatgaccca ccctgaattc
     4261 gccatagagg aggagttgcc acgaacacca ctcctggatg aggaagagga ggaaaatcct
     4321 gacaaggctt ctaagtttgg gactagggtg ctcctgttgg atggtgaggt cactccatat
     4381 gccaatacaa acaacaatgc ggtggattgc aaccaagtgc agctccccca gtcggacagc
     4441 tctctacaga gcctagagac atcagtttga attttctttc tctgactctc atccctattt
     4501 tacctatttc cattttcgtc tatcccatct atgaggtgat gatgggactt ttcattgtca
     4561 cgtcagctgc tgtgttaaca gcagtgtgtg tgaagtgaac ctctacctga ccatgaagag
     4621 gcaagagcac agacttacaa ggatttcaac ttaagcttga cttggggttt gtagcgggac
     4681 ccagtcaaac cccattcagg tcatggtaga atctactcct tggtagtcac ttgtcatttt
     4741 taaagaaatg atgacaatcc tcttggcatc accccacccc acattctccc cgatgttcct
     4801 ctcctgaatt ctggattttg tcctacaagt ctgtgccatt tataagctaa gttgagggtt
     4861 cagagggaga caaatatccc caagtgaaat atgttgcggt agagatggaa tgtataaaat
     4921 ccgccagtag tgtgattgtt tttcaatttt tcccactttg aaaacttaca cctaaaggcc
     4981 tgtaggcctc taactctttc tgccctttca ttcaaacctc tccaaagttc tttgtctttt
     5041 gtcttccctt cctttccttt tttttttttt tttttatgat gattaagaaa atggaaagga
     5101 tatggatgaa ttgtgtaaac ttagatcctt ctcccttttg gcccaaaaga acccttgatt
     5161 tagttccaga atccaaccag tttctttgtt aaaagggtcc ttttgaagca attaagtgct
     5221 ggtaagaata tttctctgtt gttgccaggt taccttctgc ttttaaattg ccatcttgta
     5281 aagtgtatgt ggttcttaag tgccagggag atcagccttg cccatgaagg ttgcatatgt
     5341 gtggtataca gtttttacct gaaagcctga gctttctctt attcctaaag tggtggcaaa
     5401 agaatgaact gggtatgacc ctgccccctt actgggcttg gatattgagg accagacgct
     5461 gccaaatcta ggacaagaca gaccatcaaa gcagactttt gtgggctcct ctttggggtg
     5521 accactgctt tcaaagccat ctgccaaggc tctccagggc aggacctgac tggtggggaa
     5581 tgagtgttca gaagccttgg gagaggccaa agagccattc tagcatgatc tgagaaaacc
     5641 ttcctgcaga ggccagaaac cttgagctta ggtgcctggg gaccagcttc gacattctct
     5701 ccagtttctg attctaattt ttgccacgtg tcacaacttt tccagtctct gagaaggtcc
     5761 cagcctttct caaatattct gattttgaaa atatgtatcc aaagtgggag gcccctgtga
     5821 cattttgcca acttaaacga gaaaaagacc ccccgcaccc ggcacactcc cccttcctcc
     5881 agccccgctt cagccacatg ctccagctgc tgcccagtaa agccctgtgc ctttttttcc
     5941 cctgaatact gcccaaagca tccccttccc atctgcctct caggagttgg ggactttgct
     6001 aggagatttt ttaagtgttc cttactggga caacgtggag ccacgtttgc aggagctcca
     6061 tttgtatccc tgctggtgtt gacttctgtg taggggccag ttcatgtccc tgactctcac
     6121 ctcccattag ataaatgaag cccacccccc tttctagagt gatgagagtc aagaagaggg
     6181 gatgtatgaa cggccaaatt cccatgtgag aggaagatga cctgatccac ctagcctttt
     6241 cttctggatc tgtcctccct cacccctttc acctgagctg tccacagtag gaaacataaa
     6301 gaaacaatgt cccctacata tccccatgac tacataatcc atcatcgtag gaaataggaa
     6361 agcaaatttg attttggttt tgtaaaacgt acatgcttca ataattcttt tttgtgtctt
     6421 aaatactcat aggggaaaaa aacagctcac ccaaggtgtt aggtttcaca tatatattca
     6481 tcaactattt tagaagattt aattctatca aatcttgtat tacctcagat cattttaaat
     6541 agcaagccaa taacgagctt tgaaggctat tttaccattc ctgttcacaa aaggttctca
     6601 tggtgcctga caggttaccc ttgagggctt gtgtctactt tttaaaagtc aatggttttt
     6661 tttcttgtgt tctagtttcc ataataggag agaaaatata gaaatatatg caaaaattat
     6721 agttttcttt agatcagaaa ctgatatttt tgggtcagcc atatgtattt tgtttaaagg
     6781 atttaaaata aagtgccgtc atgtagccct gtggaaggga gcacataacc agctgtttgg
     6841 catgacaggt gacttagtat atttgtaatt ggttttaaaa ccaatacacc atactttctt
     6901 tctgcaaaca gccatcttta tacttaggga agaaaaattg ttgggttcta gactttttta
     6961 atataaattt tgttgatatg gaattaggta agtttaagtg tctatgtgca tatgtttttt
     7021 atataagttt tttctattca gtttcactga tccaactggc agtgggtaaa tatggcataa
     7081 gttaataaca cttttcccca aaatggtgct ttggatttga aaagggtctg atggggagaa
     7141 ggagaacgta tcatcctagc ttcctctctt aataaaccta gaaaaacggg tagtaaactg
     7201 tggatagtca ggaaaacacc cagcaaggga cacagctgtc aggaaatgaa tcttcccccc
     7261 aacccccacc atgcagatgg atagacagaa tctttcctga ctagtcatta ggatcagggg
     7321 cctctgttgg atttgtgttt cttgaagaat agctggcaga gtggtataaa agacacgaat
     7381 atctcctggt ctataaggat actctgattt ggggtttgca tttttcatgg tttttatttc
     7441 ctgttccccc tggagttttc cattagtgag tttttgtgca aggatcttat ttgtgatgcc
     7501 ttccctcccc tagaaagatt ttgtgcaata tattaaatgg ggacagaatt ctaaatggat
     7561 aaaacaatgg ctggttctag ccctgagtga cagtcttaag gctagatcct tcccatagta
     7621 tcatctgtcc tctggaatga ctctcctgtc cctaaagggg ttaagagaga gatcacctag
     7681 aaatccctct ggacacttgt gggttcttta gggtttgagt ttcttcttcc ccttgagctt
     7741 cagagaggag agttggcatg gttaaatctg aatggttacc tcactgctga aaacccagag
     7801 gggcgtggca cactcgcttg tgtggaaaag cctctaaatg catcccttcc tttctttcct
     7861 gcttcctttg ccttacaatt gaagcagccc gtggtaccat cacagtatgc agagacttcc
     7921 tcacctttca tatctaggga ccacccccga tgcattggtg agggtgggca cttataaatg
     7981 cctgctattg ttaagccatt ccagcctctt cctctgaata gaccagacgc cctttcactt
     8041 agttcagtgc cagtcctttt gccttcccaa ccctgctgtt aggcctgctg ttccctttgc
     8101 tcttgattag gagagatgga aggagatgag ctcccataac tgaattggcc tttggttcat
     8161 gttttctccc catatgtata tatgccatat gtgaatatgc catatatatg tgccaacaaa
     8221 tctatctacg ttgttctttt caaattagca cgcagatagg aattttgagt ttcttcttct
     8281 tttagtaact agtataacaa gcactggtat ttttgtacaa aaaagaaaaa caaaagattg
     8341 actattgtgg tctgcatgac ataaacaaac aaatggtgat atcaaagcaa cgtatacccc
     8401 agtccagtgt gtgttgccat aatttgcaat tcagcttaac agtgcaccca atctatattt
     8461 gcattttgat attatttaag ctctatgtac aaggttttgc atgtatttat atggttctta
     8521 gggaaaaaaa atgctataaa ctgcaaatct gaaattcaaa tgtgttgttc cactgagacc
     8581 agaagaagaa gaggagtttt aaaagggata atttgttgga gccaataaag ctttttgctg
     8641 atgaacagaa accaatactg ctgtgcactg agaataaaaa ctcatgccca cttgtaa
//
