LOCUS       NM_001001331            8964 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens ATPase plasma membrane Ca2+ transporting 2 (ATP2B2),
            transcript variant 1, mRNA.
ACCESSION   NM_001001331
VERSION     NM_001001331.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Poggio,E., Barazzuol,L., Salmaso,A., Milani,C.,
            Deligiannopoulou,A., Cazorla,A.G., Jang,S.S., Julia-Palacios,N.,
            Keren,B., Kopajtich,R., Lynch,S.A., Mignot,C., Moorwood,C.,
            Neuhofer,C., Nigro,V., Oostra,A., Prokisch,H., Saillour,V.,
            Schuermans,N., Torella,A., Verloo,P., Yazbeck,E., Zollino,M.,
            Jech,R., Winkelmann,J., Necpal,J., Cali,T., Brini,M. and Zech,M.
  TITLE     ATP2B2 de novo variants as a cause of variable neurodevelopmental
            disorders that feature dystonia, ataxia, intellectual disability,
            behavioral symptoms, and seizures
  JOURNAL   Genet Med 25 (12), 100971 (2023)
   PUBMED   37675773
  REMARK    GeneRIF: ATP2B2 de novo variants as a cause of variable
            neurodevelopmental disorders that feature dystonia, ataxia,
            intellectual disability, behavioral symptoms, and seizures.
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Smits,J.J., Oostrik,J., Beynon,A.J., Kant,S.G., de Koning
            Gans,P.A.M., Rotteveel,L.J.C., Klein Wassink-Ruiter,J.S.,
            Free,R.H., Maas,S.M., van de Kamp,J., Merkus,P., Koole,W.,
            Feenstra,I., Admiraal,R.J.C., Lanting,C.P., Schraders,M.,
            Yntema,H.G., Pennings,R.J.E. and Kremer,H.
  CONSRTM   DOOFNL Consortium
  TITLE     De novo and inherited loss-of-function variants of ATP2B2 are
            associated with rapidly progressive hearing impairment
  JOURNAL   Hum Genet 138 (1), 61-72 (2019)
   PUBMED   30535804
  REMARK    GeneRIF: Loss-of-function variants of ATP2B2 are associated with
            rapidly progressive hearing impairment.
REFERENCE   3  (bases 1 to 8964)
  AUTHORS   Sundell,G.N., Arnold,R., Ali,M., Naksukpaiboon,P., Orts,J.,
            Guntert,P., Chi,C.N. and Ivarsson,Y.
  TITLE     Proteome-wide analysis of phospho-regulated PDZ domain interactions
  JOURNAL   Mol Syst Biol 14 (8), e8129 (2018)
   PUBMED   30126976
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8964)
  AUTHORS   Vicario,M., Zanni,G., Vallese,F., Santorelli,F., Grinzato,A.,
            Cieri,D., Berto,P., Frizzarin,M., Lopreiato,R., Zonta,F., Ferro,S.,
            Sandre,M., Marin,O., Ruzzene,M., Bertini,E., Zanotti,G., Brini,M.,
            Cali,T. and Carafoli,E.
  TITLE     A V1143F mutation in the neuronal-enriched isoform 2 of the PMCA
            pump is linked with ataxia
  JOURNAL   Neurobiol Dis 115, 157-166 (2018)
   PUBMED   29655659
  REMARK    GeneRIF: A V1143F substitution in PMAC2 alters the binding of
            caslmodulin to the calmodulin-binding domain leading to impaired
            calcium signaling.
REFERENCE   5  (bases 1 to 8964)
  AUTHORS   Roome,C.J. and Empson,R.M.
  TITLE     Survival strategies for mouse cerebellar Purkinje neurons lacking
            PMCA2
  JOURNAL   Neurosci Lett 663, 25-28 (2018)
   PUBMED   29452612
  REMARK    GeneRIF: PMCA2 loss plays a role in the ataxic phenotype, but can
            paradoxically also minimise calcium rises in cerebellar Purkinje
            neurons, thereby ensuring their resilience and survival.
            Review article
REFERENCE   6  (bases 1 to 8964)
  AUTHORS   Hilfiker,H., Guerini,D. and Carafoli,E.
  TITLE     Cloning and expression of isoform 2 of the human plasma membrane
            Ca2+ ATPase. Functional properties of the enzyme and its splicing
            products
  JOURNAL   J Biol Chem 269 (42), 26178-26183 (1994)
   PUBMED   7929331
REFERENCE   7  (bases 1 to 8964)
  AUTHORS   Kuzmin,I., Stackhouse,T., Latif,F., Duh,F.M., Geil,L., Gnarra,J.,
            Yao,M., Orcutt,M.L., Li,H., Tory,K. et al.
  TITLE     One-megabase yeast artificial chromosome and 400-kilobase
            cosmid-phage contigs containing the von Hippel-Lindau tumor
            suppressor and Ca(2+)-transporting adenosine triphosphatase isoform
            2 genes
  JOURNAL   Cancer Res 54 (9), 2486-2491 (1994)
   PUBMED   8162598
REFERENCE   8  (bases 1 to 8964)
  AUTHORS   Brandt,P., Ibrahim,E., Bruns,G.A. and Neve,R.L.
  TITLE     Determination of the nucleotide sequence and chromosomal
            localization of the ATP2B2 gene encoding human Ca(2+)-pumping
            ATPase isoform PMCA2
  JOURNAL   Genomics 14 (2), 484-487 (1992)
   PUBMED   1427863
REFERENCE   9  (bases 1 to 8964)
  AUTHORS   Heim,R., Hug,M., Iwata,T., Strehler,E.E. and Carafoli,E.
  TITLE     Microdiversity of human-plasma-membrane calcium-pump isoform 2
            generated by alternative RNA splicing in the N-terminal coding
            region
  JOURNAL   Eur J Biochem 205 (1), 333-340 (1992)
   PUBMED   1313367
REFERENCE   10 (bases 1 to 8964)
  AUTHORS   Wang,K.K., Wright,L.C., Machan,C.L., Allen,B.G., Conigrave,A.D. and
            Roufogalis,B.D.
  TITLE     Protein kinase C phosphorylates the carboxyl terminus of the plasma
            membrane Ca(2+)-ATPase from human erythrocytes
  JOURNAL   J Biol Chem 266 (14), 9078-9085 (1991)
   PUBMED   1827443
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB210008.1, AK308898.1,
            S95738.1, X63575.1 and AC022384.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001001331.3.
            
            Summary: The protein encoded by this gene belongs to the family of
            P-type primary ion transport ATPases characterized by the formation
            of an aspartyl phosphate intermediate during the reaction cycle.
            These enzymes remove bivalent calcium ions from eukaryotic cells
            against very large concentration gradients and play a critical role
            in intracellular calcium homeostasis. The mammalian plasma membrane
            calcium ATPase isoforms are encoded by at least four separate genes
            and the diversity of these enzymes is further increased by
            alternative splicing of transcripts. The expression of different
            isoforms and splice variants is regulated in a developmental,
            tissue- and cell type-specific manner, suggesting that these pumps
            are functionally adapted to the physiological needs of particular
            cells and tissues. This gene encodes the plasma membrane calcium
            ATPase isoform 2. Alternatively spliced transcript variants
            encoding different isoforms have been identified. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1), also known as WB.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: X63575.1, SRR1803617.205592.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000360273.7/ ENSP00000353414.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AB210008.1         72-73
            3-1043              AK308898.1         1-1041
            1044-1348           AB210008.1         1115-1419
            1349-1620           S95738.1           978-1249
            1621-4424           X63575.1           1250-4053
            4425-8964           AC022384.5         203225-207764       c
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..8964
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /note="ATPase plasma membrane Ca2+ transporting 2"
                     /db_xref="GeneID:491"
                     /db_xref="HGNC:HGNC:815"
                     /db_xref="MIM:108733"
     exon            1..122
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            123..640
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     CDS             442..4173
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /EC_number="7.2.2.10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     plasma membrane Ca2+ pump 2; plasma membrane calcium pump;
                     plasma membrane calcium-transporting ATPase 2; plasma
                     membrane calcium ATPase; plasma membrane Ca(2+)-ATPase;
                     ATPase, Ca++ transporting, plasma membrane 2"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 2
                     isoform 1"
                     /protein_id="NP_001001331.1"
                     /db_xref="CCDS:CCDS33701.1"
                     /db_xref="GeneID:491"
                     /db_xref="HGNC:HGNC:815"
                     /db_xref="MIM:108733"
                     /translation="MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVV
                     KIKETYGDTEAICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEA
                     LQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGGAEDEGEAEAGWIEGAAILLS
                     VICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQVKY
                     GDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAV
                     GVNSQTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNG
                     KMQDGNVDASQSKAKQQDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTK
                     LAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLPECTPVYVQYFVKFFIIGVTV
                     LVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNR
                     MTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGALPRQVG
                     NKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYS
                     KGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSS
                     PEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARA
                     IAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHT
                     LVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDD
                     NFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVN
                     LIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFV
                     GEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNP
                     IFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSR
                     LKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKA
                     FRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPS
                     SLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL"
     exon            641..838
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            839..1096
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1222
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1348
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1381
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1441
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1483
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..1642
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1857
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..2100
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            2101..2342
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            2343..2577
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2757
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2845
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            2846..2952
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            2953..3144
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            3145..3358
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3570
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            3571..3678
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            3679..3861
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..8964
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8941..8946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /note="hexamer: AATAAA"
     polyA_site      8964
                     /gene="ATP2B2"
                     /gene_synonym="DFNA82; PMCA2; PMCA2a; PMCA2i"
                     /note="major polyA site"
ORIGIN      
        1 acagtctgca gaggggcccg ggcacgctgg ctgggctcag ccgcggcacc agctgccgca
       61 ccgcgattac ggggctgccc gccgggaaac cgtgcaggat tggtggtgat tttcatagcc
      121 aagagccacc acccctgacc atgtagatgc cagttccagg gagcagcatg ggccccactg
      181 aatggagact cctgggtcta cagccctgag cccctccggc ccctggacct cgtcccacac
      241 cggaggacac ctcttggagc tcaccaccac tgtcaccagc ccgcctcggc cacccccacc
      301 ccccgggacc cggagtcggc cgcctggtgc cacagctgac cagtgagggt gtgctgagga
      361 cagccacaag cagccatcac ccggcagcct cttgtccagc gctgaccctt gggcccagcc
      421 cgagcaagga ccgcagcaaa catgggtgac atgaccaaca gcgactttta ctccaaaaac
      481 caaagaaatg agtcgagcca tgggggcgag ttcgggtgca caatggagga gctccgctcc
      541 ctcatggagc tgcggggcac tgaggctgtg gtcaagatca aggagactta tggggacacc
      601 gaagccatct gccggcgcct caaaacctca cctgttgaag gtttgccggg caccgctcca
      661 gacctggaaa agagaaagca aatttttggg caaaacttta tacctccaaa gaagccaaaa
      721 accttcctgc agctcgtgtg ggaggcgctg caggacgtga cgctcatcat cctggagatt
      781 gccgccatca tctccctggg gctgtccttc taccacccgc ccggcgaggg caacgaagga
      841 tgtgcgacgg cccagggtgg ggcagaggat gaaggagagg cagaggcagg ttggatcgag
      901 ggggccgcca ttctcctctc agttatctgt gtggtcctgg tcacggcctt caatgactgg
      961 agcaaagaga aacagttccg gggcctgcag agccgcatcg agcaggaaca gaaatttacc
     1021 gtggtccggg ctggccaggt ggtccagatc cctgtggctg agatcgtggt tggggacata
     1081 gcccaggtca aatatggtga cctcctccct gccgacggcc tcttcatcca gggcaatgac
     1141 ctcaagattg atgaaagctc cctaactgga gagtctgacc aggtgcgcaa gtccgtggac
     1201 aaggacccca tgctgctgtc aggaacccac gtgatggagg gctcaggacg gatgttggtg
     1261 actgctgtgg gtgtgaactc tcagactggc atcatcttta ccctcctggg ggctggtggt
     1321 gaagaggaag agaagaaaga caaaaaaggt gtgaagaagg gggatggcct tcagctacca
     1381 gcagcagacg gtgcggcagc ttcaaatgct gcagatagtg cgaatgccag cctagtcaat
     1441 ggtaaaatgc aggatggcaa tgtggacgcc agccagagca aagccaaaca acaggacggg
     1501 gcagccgcca tggagatgca gcccctcaag agtgccgagg gcggcgacgc tgacgacagg
     1561 aagaaggcca gcatgcacaa gaaggagaag tccgtgctgc agggcaagct caccaagctg
     1621 gctgtgcaga tcgggaaggc gggcttggtg atgtcagcca tcacggtgat catcctggtg
     1681 ctctacttca ctgtggacac cttcgtggtc aacaagaagc cgtggctgcc tgagtgcacg
     1741 cccgtctacg tgcagtactt tgtcaagttc ttcatcattg gcgtgacggt gctggtggtc
     1801 gccgtgcccg aggggctccc tctggccgtc accatctcgt tggcctattc ggtgaagaaa
     1861 atgatgaagg acaacaacct ggtacgccac ctggatgcct gtgagaccat gggcaatgcc
     1921 acagccatct gctcagacaa gacaggcacg ctgaccacca atcgcatgac agtggtacag
     1981 gcctatgtcg gcgacgtcca ctataaagag atccccgacc ccagctccat caacaccaag
     2041 accatggagc tgctgatcaa tgccatcgcc atcaacagcg cctacaccac caagattctg
     2101 cccccagaga aggagggcgc cctgcctcgg caggtgggca acaagacgga gtgcggcctg
     2161 ctgggcttcg tgctggacct gaagcaggac tacgagcccg tgcgcagcca gatgccagag
     2221 gagaagttgt acaaagtgta caccttcaac tccgtgcgca agtccatgag cactgtcatc
     2281 aagctgcccg acgagagctt ccgcatgtac agcaaggggg cttctgagat cgtgctcaag
     2341 aagtgctgca aaatcctcaa tggggcggga gagcctcgtg tcttccggcc ccgcgaccgg
     2401 gacgagatgg taaagaaggt gattgagccc atggcttgcg atgggctccg cactatctgc
     2461 gtggcctacc gcgacttccc cagcagcccg gagccggact gggacaatga gaatgacatc
     2521 ctcaacgaac tcacctgcat ctgcgtggtg ggcatcgagg acccggtgcg gccagaggtc
     2581 ccagaagcca tccgcaagtg ccagcgggca ggcatcacgg tccgcatggt cactggcgac
     2641 aatatcaaca cggctcgggc catcgccatc aagtgtggca tcatccatcc tggggaggac
     2701 tttctgtgcc tcgagggcaa ggagttcaac aggaggatcc gcaacgagaa gggggagatt
     2761 gagcaggagc gaattgacaa gatctggcca aagctgcggg tgctggctcg ctcctcccca
     2821 acggacaagc ataccctggt taaaggcatc atcgacagca cacacactga gcagcggcag
     2881 gtggtggccg tgacggggga cgggaccaac gacgggcctg cactcaagaa ggccgacgtg
     2941 ggcttcgcca tgggcatcgc aggcactgac gtggccaagg aggcctcaga catcatcctg
     3001 acagacgaca atttcagcag catcgtcaag gcagtgatgt ggggccgcaa cgtctatgac
     3061 agcatctcca aattcttgca gttccagctc accgtcaacg tggtggccgt gattgtggcc
     3121 ttcacaggcg cctgcatcac gcaggactcc cctctgaagg ccgtgcagat gctctgggtg
     3181 aacctcatca tggacacgtt tgcctcgctg gcactggcca ctgagccgcc cacggagacc
     3241 ctgctgctga ggaagccgta cggccgcaac aagccgctca tctccaggac catgatgaag
     3301 aacatcctgg gccatgctgt ctaccagctt gccctcatct tcaccctgct ctttgttggc
     3361 gagaagatgt tccagatcga cagcgggagg aacgcgcccc tgcattcgcc accctcagaa
     3421 cattacacca tcatcttcaa caccttcgtc atgatgcagc tcttcaacga gatcaacgcc
     3481 cgcaagatcc acggcgagcg caatgtcttt gacggcatct tccggaaccc catcttctgc
     3541 accatcgtgc tgggcacctt tgccatccag atagtgatcg tgcagtttgg agggaagcca
     3601 ttcagctgct ctccactgca gctggaccag tggatgtggt gcatattcat tgggttagga
     3661 gagctcgttt ggggccaggt catcgccacc atcccgacca gcagactcaa gttcctcaag
     3721 gaggcaggca ggctcacaca gaaggaggag atcccggagg aggagctcaa cgaggacgtg
     3781 gaggagatcg accacgcgga gcgggagctg cggcggggcc agatcctgtg gttccgaggc
     3841 ctgaatcgga tccagacaca gatccgcgtc gtgaaggcgt tccgtagctc tctctatgaa
     3901 ggtttagaaa agcctgaatc tcgaacctcc atccataact tcatggctca tcctgaattc
     3961 cggatcgaag attcccagcc ccacatcccc ctcattgatg acaccgacct ggaagaagat
     4021 gccgcgctca agcagaactc gagcccgccg tcatccctca acaagaacaa cagcgccatc
     4081 gacagtggga tcaacctgac gaccgacaca agcaaatcag ctacctcttc aagtccaggg
     4141 agccccatcc acagcctgga gacgtcgctt tagctgagga ccctctcgcc tgcccgcccg
     4201 ccctcatgga ccccgctgcc acccgctttc cgggcaccca tccatccagg cacccaactc
     4261 acccaagcag caacgagcaa caatcggaaa ccaaatactg gagagaaaac caacgtttcc
     4321 acccacagac cctttctctg gctgcgatgc tgtttgaact ctttttcact tcaaggcaag
     4381 gggcgggatc tccactgggg gcttacggga gtgagcggtt ttcccaaaac aagcccttcc
     4441 tggctcccac ccagacatgg accagccatg cacccgccca gccaccacgt cccccgcatg
     4501 aatgtactgt acactttcaa tcctcccctt gtttggtttt tgggggttgg ggaggggttt
     4561 ttgtttgttt gtttgttttc ttaggcggga actgcaaaca gactcttttc tgagactatt
     4621 tatccaatcc actggtctgt gagtttttga aatgcttgca cagcatggtc tcagttgtat
     4681 agattaattt aataactttt tgaaattgca gagcttaact cgcctagtag atttgcacca
     4741 atggaaccga agaacttcat agacactcac aaggttatat ccatttcttt gtatctatat
     4801 caacgtatac ttttccgaga ctgtatacgt ccatatagat aggtagatat atatatatat
     4861 atataaatat atatacatgg atatataaag tttctttgcc ggcatgttgc cttgtttccg
     4921 cttaaattgc tctattttaa cttatttatg tcctaaaaga agaatgtaat ttgtttacaa
     4981 acctgtagat aacgtctttg gctatttgta tggtttaaga acactggtgg ctgagatgct
     5041 ataaaaacag ctcggcccaa cagacacttc ccctgggttg catcctggat gttttatgat
     5101 agccatgctc tgatttttgc ctgctatttc cgttcaataa tgtcactacc gtgagaggct
     5161 caggcagaag ccaaatgcta ccgagttgcc atcctgaggg gtttaacaac atgctccgta
     5221 gacgaaggga gaggaggaga gaaggcttcc tgggtttgca acactaacgg ccatccggcc
     5281 caaggatgcc aggatctgca aagcactgct cgaagacttt tctctcaatg aaactcgctt
     5341 gagtttacta agagcatttc aaaaataggt tctctttggc actgtctgta cagagattga
     5401 ggtagtgttg aaatattata aatggtattg tgttgatttt tttttttatt tagtaactta
     5461 caggtttgtt tccttattaa tggcagcatc tgagctgttg gcatattgga tgaggatcag
     5521 tatggcttgc tgcttttatt tttatttttg aagaagaata gcctttttct ctgcactatt
     5581 tagatccgaa tgaaccttat gatgtgtata ttgagatgta ctcagtgtga ttttaaacca
     5641 aattgtcttc ctgtagtcac aatatatact gtagcctttt aacagcaagt cttgctttcc
     5701 caaacagaaa gccattctga aaccctacag tatcacaggt gagaaaaggt ggttattttt
     5761 tccccaagac aacagcacta gtaatcccac ttaataagag cttatttaat tggatgtcag
     5821 cctcttaact gctaagcact ttgtgggtct cagcgttttt cataaaagaa cttttgtatt
     5881 taatacaaag tttgctttga gacttttcag catatgatct tttttccata aacttgtaca
     5941 gtgcaaaaga cattttgaat accatgatcg atgatgtccc atgcttcgag gaaaaccaaa
     6001 cactttccgc ctctcttgca aaatccattc ctcatgctga ccctcctcac gatggctgtg
     6061 tcagcccagc cccttccctt ctccaggccc agagaactct tccacaaaca agatgagagc
     6121 cactcgggaa aagagccata gtcaactggg agggcctaca tctggatggc ggtggaaaaa
     6181 cttgagggtt tggggttcaa agtcagccca tcccacctgg caaaatcctc ctggaaggag
     6241 gaccttcaag agcgcatcac ctgaatgtcg tgaagaagta tctctgaatg tatccaggag
     6301 aggaactgca taaccaaagg ggtgaccagc cctcagatgt gcttattgga ttccagtaca
     6361 aacgccacca aagccagccc actgctctcc tacaaggaag gaaagatctg cacgtgtaaa
     6421 acatggggca gccttggaac atggtgtttt ttggagtttc ctttctcaca gttttccatc
     6481 tccccacttc tttgatcagt catgtgtccg tgacctcgtt ccatgacatc aggatagctg
     6541 tgtttgcaca ccatgctcca tgttcattcg gagccaggag gggttctcag tggagcctgg
     6601 cttagggaac agggagcgat ggaagaatgc caacattagc gttggtcttc tcttgtcagg
     6661 aatgaaggat gcttgcacac atgcaccccc tcactctcac acttgcacac atacacacac
     6721 acacacacga aatggttggt ttgtcaaaac tcactgtagt acataaagct tgcactctgc
     6781 gtcctatatc tagcagcatg gggtacgttt ggcagttcac tccattaggg ggtaaataat
     6841 ttatgaccat tcatctgttt ttatgaattt ttttatctag acaataatgt aaataaagaa
     6901 ctcaccatct ctgttcattt aatactatgc aatggttatg ctttcaatcg ctggctcttc
     6961 tgactcgtgc agtgtggttc tgaaatgttt gtggtttaaa aaaaaaagca aaaaacaaca
     7021 tcaaacagaa catagtaaat atatctgtaa tgtatacttt tttctacttt gggactgcat
     7081 catccacata gcttcttttc tgtcctctag acaagggctc caggagtaag tggcagattt
     7141 ggttctgaga gctaagctca ccaacttgtc tccccctcaa cagatgaggg aagggcaggg
     7201 agcccgttca gtgactcctg gcgctgcaga atcactcatc tttggaggtg agcagagaga
     7261 gctctgagga gtttccttct tgcaactcag gcggagcttt ctttaactca ggagcagcct
     7321 ccattgctcc cgccaacccc tcaccatggc cccttgtccc cttgtcccaa ccatagcata
     7381 gagatgacaa gagagaggca gtcctatttc tgagactgga ccaattaaaa tgactgcact
     7441 ggctcaaagg actctgaacc tgtcttgtcc tgggtcctcc agttgttgga agccactctt
     7501 ttccccaacc atgctgaaat gccaccttta ccacacactg atgtcttaca tagacttaga
     7561 tctatttctg gaacttctct tctcttcgct tacttgggtg tctcatctgg caccagccat
     7621 acaattttaa ctattgtcaa tttctaatgt cttattatgt ggtgggatga gtcccttcac
     7681 tcttctttta caaaatttcc tagttattct ctcacatcta ttcttccaca taaactttaa
     7741 attgtgccca gtcctccacc ccctgtccca ttcccacccc caaattctgg gctctgtcat
     7801 tccctgtgcc atcatcttcc ccccattgta attactgtcc actcagggct gtgaaataga
     7861 ctatgttctg taaagaggga caaaaagggc atcctgatct tgagagaagc ctgctgttcc
     7921 tcccatcatt tcacctgccc cctaagccca ggagttgtca tgagaggtca ccaggccccc
     7981 actcaatctc agcttgggaa ccaaagtcac ccaccttggt tgtgttgggg tggacccgcc
     8041 atctgtccct ggtccagacg agaaagagga gtctctccta ggcctggagc tgggaaagaa
     8101 tgtgtggccc agttgtctgc acttctaatt ctcatcatgg agaaaccttt attcctatct
     8161 ccctttcctg aactggtttt ttgttgtttt tgtttcattt tgttttgggg ggatagtttc
     8221 ttgctcttta atttggagtc tccagtacct ttgggatgca ggcagttctt gcctgggcct
     8281 tctcggaacc ctcactcccc tagcccactc ttgcgctacc tgcaggaggc tgccaacctg
     8341 gtgcattctg acaagcctcc cacccaaatc tctctcctgc cattgtgtcc aaaatcccac
     8401 cattagaggc tcttgtaggg aagagcgttt cttgaaggct tttaggcctt ccagagccag
     8461 gagggaagtc agacaatagc aggaagtccc caggcctttt caaagttcca aaccaagctc
     8521 tcctgatttt aatgtagaga tcataccaac ccaggtgggg gaggagggtc cccagcccca
     8581 ggcagcagcc atcaccccct ccactgaaaa caatattgga ggctgctttg ggactgccct
     8641 tctcagcccc ctaagtctgt tttgtaatgc ctgtggtgct ctccctcctg gacctttcct
     8701 ctcgggggtc accacacttt gctaactctt gtgtgcacat attttataat agagtagcga
     8761 gggaatggtg ccgcctccag cttccgtaag ctgcccgggc tctggggggc tctgggacaa
     8821 tcggggctgg gaagtgactg tgctcttatt gtacactctt tatttctctg tatctttggc
     8881 ttgtgctctt tgtaattaat gggatttgtc tgccttttca acactatact gagcaataac
     8941 aataaatgca cacgtggaaa tgca
//
