LOCUS       NM_001413060            6406 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens ATPase plasma membrane Ca2+ transporting 1 (ATP2B1),
            transcript variant 30, mRNA.
ACCESSION   NM_001413060
VERSION     NM_001413060.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Kobayashi,Y., Yatsu,K., Haruna,A., Kawano,R., Ozawa,M., Haze,T.,
            Komiya,S., Suzuki,S., Ohki,Y., Fujiwara,A., Saka,S., Hirawa,N.,
            Toya,Y. and Tamura,K.
  TITLE     ATP2B1 gene polymorphisms associated with resistant hypertension in
            the Japanese population
  JOURNAL   J Clin Hypertens (Greenwich) 26 (4), 355-362 (2024)
   PUBMED   38430457
  REMARK    GeneRIF: ATP2B1 gene polymorphisms associated with resistant
            hypertension in the Japanese population.
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Gao,Y., Liu,Y., Jiang,Y., Qin,M., Guan,Z., Gao,Y. and Yang,Y.
  TITLE     Association between polymorphism and haplotype of ATP2B1 gene and
            skeletal fluorosis in Han population
  JOURNAL   Int J Environ Health Res 34 (3), 1410-1420 (2024)
   PUBMED   37211801
  REMARK    GeneRIF: Association between polymorphism and haplotype of ATP2B1
            gene and skeletal fluorosis in Han population.
REFERENCE   3  (bases 1 to 6406)
  AUTHORS   Berrocal,M. and Mata,A.M.
  TITLE     The Plasma Membrane Ca2+-ATPase, a Molecular Target for Tau-induced
            Cytosolic Calcium Dysregulation
  JOURNAL   Neuroscience 518, 112-118 (2023)
   PUBMED   35469971
  REMARK    GeneRIF: The Plasma Membrane Ca[2+]-ATPase, a Molecular Target for
            Tau-induced Cytosolic Calcium Dysregulation.
            Review article
REFERENCE   4  (bases 1 to 6406)
  AUTHORS   Yang,X., Lu,L., Wu,C. and Zhang,F.
  TITLE     ATP2B1-AS1 exacerbates sepsis-induced cell apoptosis and
            inflammation by regulating miR-23a-3p/TLR4 axis
  JOURNAL   Allergol Immunopathol (Madr) 51 (2), 17-26 (2023)
   PUBMED   36916084
  REMARK    GeneRIF: ATP2B1-AS1 exacerbates sepsis-induced cell apoptosis and
            inflammation by regulating miR-23a-3p/TLR4 axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6406)
  AUTHORS   Rahimi,M.J., Urban,N., Wegler,M., Sticht,H., Schaefer,M., Popp,B.,
            Gaunitz,F., Morleo,M., Nigro,V., Maitz,S., Mancini,G.M.S.,
            Ruivenkamp,C., Suk,E.K., Bartolomaeus,T., Merkenschlager,A.,
            Koboldt,D., Bartholomew,D., Stegmann,A.P.A., Sinnema,M.,
            Duynisveld,I., Salvarinova,R., Race,S., de Vries,B.B.A.,
            Trimouille,A., Naudion,S., Marom,D., Hamiel,U., Henig,N.,
            Demurger,F., Rahner,N., Bartels,E., Hamm,J.A., Putnam,A.M.,
            Person,R., Abou Jamra,R. and Oppermann,H.
  TITLE     De novo variants in ATP2B1 lead to neurodevelopmental delay
  JOURNAL   Am J Hum Genet 109 (5), 944-952 (2022)
   PUBMED   35358416
  REMARK    GeneRIF: De novo variants in ATP2B1 lead to neurodevelopmental
            delay.
REFERENCE   6  (bases 1 to 6406)
  AUTHORS   Kessler,F., Falchetto,R., Heim,R., Meili,R., Vorherr,T.,
            Strehler,E.E. and Carafoli,E.
  TITLE     Study of calmodulin binding to the alternatively spliced C-terminal
            domain of the plasma membrane Ca2+ pump
  JOURNAL   Biochemistry 31 (47), 11785-11792 (1992)
   PUBMED   1332771
REFERENCE   7  (bases 1 to 6406)
  AUTHORS   Wang,K.K., Wright,L.C., Machan,C.L., Allen,B.G., Conigrave,A.D. and
            Roufogalis,B.D.
  TITLE     Protein kinase C phosphorylates the carboxyl terminus of the plasma
            membrane Ca(2+)-ATPase from human erythrocytes
  JOURNAL   J Biol Chem 266 (14), 9078-9085 (1991)
   PUBMED   1827443
REFERENCE   8  (bases 1 to 6406)
  AUTHORS   Olson,S., Wang,M.G., Carafoli,E., Strehler,E.E. and McBride,O.W.
  TITLE     Localization of two genes encoding plasma membrane
            Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and
            12q21-23
  JOURNAL   Genomics 9 (4), 629-641 (1991)
   PUBMED   1674727
REFERENCE   9  (bases 1 to 6406)
  AUTHORS   Strehler,E.E., Strehler-Page,M.A., Vogel,G. and Carafoli,E.
  TITLE     mRNAs for plasma membrane calcium pump isoforms differing in their
            regulatory domain are generated by alternative splicing that
            involves two internal donor sites in a single exon
  JOURNAL   Proc Natl Acad Sci U S A 86 (18), 6908-6912 (1989)
   PUBMED   2528729
REFERENCE   10 (bases 1 to 6406)
  AUTHORS   James,P.H., Pruschy,M., Vorherr,T.E., Penniston,J.T. and
            Carafoli,E.
  TITLE     Primary structure of the cAMP-dependent phosphorylation site of the
            plasma membrane calcium pump
  JOURNAL   Biochemistry 28 (10), 4253-4258 (1989)
   PUBMED   2548572
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009522.11, AC068641.18 and
            AC025034.22.
            
            Summary: The protein encoded by this gene belongs to the family of
            P-type primary ion transport ATPases characterized by the formation
            of an aspartyl phosphate intermediate during the reaction cycle.
            These enzymes remove bivalent calcium ions from eukaryotic cells
            against very large concentration gradients and play a critical role
            in intracellular calcium homeostasis. The mammalian plasma membrane
            calcium ATPase isoforms are encoded by at least four separate genes
            and the diversity of these enzymes is further increased by
            alternative splicing of transcripts. The expression of different
            isoforms and splice variants is regulated in a developmental,
            tissue- and cell type-specific manner, suggesting that these pumps
            are functionally adapted to the physiological needs of particular
            cells and tissues. This gene encodes the plasma membrane calcium
            ATPase isoform 1. Alternatively spliced transcript variants
            encoding different isoforms have been identified. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038196.574102.1,
                                           SRR14038194.3098552.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AC009522.11        112886-113033
            149-577             AC068641.18        39483-39911
            578-775             AC068641.18        53235-53432
            776-937             AC068641.18        68975-69136
            938-1152            AC068641.18        71193-71407
            1153-1395           AC068641.18        73799-74041
            1396-1637           AC068641.18        75350-75591
            1638-1875           AC068641.18        78551-78788
            1876-2055           AC068641.18        84218-84397
            2056-2143           AC068641.18        85082-85169
            2144-2250           AC068641.18        85547-85653
            2251-2442           AC068641.18        91244-91435
            2443-2656           AC068641.18        91665-91878
            2657-2868           AC068641.18        92336-92547
            2869-2976           AC068641.18        94157-94264
            2977-3159           AC068641.18        96291-96473
            3160-3386           AC068641.18        104295-104521
            3387-6406           AC025034.22        1-3020
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.33"
     gene            1..6406
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="ATPase plasma membrane Ca2+ transporting 1"
                     /db_xref="GeneID:490"
                     /db_xref="HGNC:HGNC:814"
                     /db_xref="MIM:108731"
     exon            1..148
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            149..577
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     CDS             370..3471
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /EC_number="7.2.2.10"
                     /note="isoform 15 is encoded by transcript variant 30;
                     plasma membrane calcium pump; ATPase, Ca++ transporting,
                     plasma membrane 1; plasma membrane calcium-transporting
                     ATPase 1"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 1
                     isoform 15"
                     /protein_id="NP_001399989.1"
                     /db_xref="GeneID:490"
                     /db_xref="HGNC:HGNC:814"
                     /db_xref="MIM:108731"
                     /translation="MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTD
                     ALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLV
                     WEALQDVTLIILEIAAIVSLGLSFYQPPEGDNAPKAQDGAAMEMQPLKSEEGGDGDEK
                     DKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWL
                     AECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDAC
                     ETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVN
                     CAYTSKILPPEKEGGLPRHVGNKTECALLGLLLDLKRDYQDVRNEIPEEALYKVYTFN
                     SVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVI
                     EPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGIEDPVRPEVPDAIK
                     KCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQE
                     RIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSDQRQVVAVTGDGTNDGPALKKADVG
                     FAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIV
                     AFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLRKPYGRNKPLISRT
                     MMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHYTIVFNTFVLMQLF
                     NEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKPFSCSELSIEQWLW
                     SIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAEDVEEIDHAERELR
                     RGQILWFRGLNRIQTQIRVVNAFRSSLYEGLEKPESRSSIHNFMTHPEFRIEDSEPHI
                     PLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEMNKSATSSSPGSPLHSLE
                     TSL"
     exon            578..775
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            776..937
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1152
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1395
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1637
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1875
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2055
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2056..2143
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2250
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2442
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2656
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..2868
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2869..2976
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3159
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..6406
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4625..4630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="hexamer: AATAAA"
     polyA_site      4651
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="major polyA site"
     regulatory      6378..6383
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="hexamer: AATAAA"
     regulatory      6382..6387
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="hexamer: AATATA"
     polyA_site      6400
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
     polyA_site      6406
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="major polyA site"
ORIGIN      
        1 gtcacgtgac cgcgctcagc caaggcggca gcggcggcgg cgcgagcttc ggcggcggtg
       61 gcggctgtgg cagcgggagc agctgcgccg ccagcgtgga gcgggaggcg tgtgggtgtg
      121 gagaaaaggg agcgccagac cggaacggat gtgtatatct catgattgat atggagaaac
      181 tagtcatggg ccaaaggtca agatacttct ctgggaaatg ttgctgctga tgctgcttta
      241 caaagtcata caatgagtgt ttggtttaag aaagattttc atacttaaaa gattttcatc
      301 ttggaaatac atcaagtgaa aattaaattc ttttgggaaa cattttcctt ctgatatatt
      361 atacttgtaa tgggcgacat ggcaaacaac tcagttgctt acagtggtgt gaaaaactct
      421 ttgaaggaag ctaatcatga tggagacttt ggaattacgc tcgcagagct gcgggctctc
      481 atggagctca ggtccacaga tgcattacga aaaatacagg aaagctatgg agatgtctat
      541 ggaatttgca ccaaattgaa aacatctccc aatgaaggtt taagtggaaa ccctgcagat
      601 ttagaaagaa gagaagcagt gtttggaaag aattttatac ctcctaaaaa gccaaaaacc
      661 tttcttcaat tagtatggga agcattacaa gatgtcactt taattatatt agaaattgca
      721 gccatagtat cattgggcct ttctttttat cagcctccag aaggggataa tgcaccaaaa
      781 gcccaggatg gtgcagccat ggaaatgcag ccattgaaga gtgaagaagg tggagatggt
      841 gatgaaaaag ataaaaagaa agcaaatttg ccaaaaaagg aaaaatctgt tttacaaggg
      901 aaacttacaa aactggctgt tcagattggc aaagcaggtc tgttgatgtc tgccatcaca
      961 gttatcattc tagtattata ttttgtcatt gacaccttct gggttcagaa aagaccatgg
     1021 cttgctgagt gcacaccaat ttatatacaa tactttgtga agttcttcat tattggagtt
     1081 acagttttag tggtcgcagt gccagaaggt cttccacttg cagtcacgat ctcactggct
     1141 tattcagtca aaaaaatgat gaaagataat aacttagtaa ggcatctgga tgcttgtgaa
     1201 accatgggaa atgctacagc tatttgttca gataaaacag gaactttgac aatgaacaga
     1261 atgacagtcg ttcaagctta cataaatgaa aaacattata aaaaggttcc tgaaccagaa
     1321 gctattccac caaatatttt gtcctatctt gtaacaggaa tttctgtgaa ttgtgcttat
     1381 acatcaaaaa tattgccacc agagaaagag ggtggattac ctcgtcacgt tggtaataaa
     1441 actgaatgtg ccttgttggg acttcttttg gatttaaaac gggattatca ggatgttaga
     1501 aatgaaatac cagaagaagc actgtacaaa gtctacacct tcaattctgt taggaagtcc
     1561 atgagtactg tcctgaaaaa ttcagatgga agttatcgaa tattcagcaa gggtgcatct
     1621 gagataattc tgaaaaagtg tttcaaaatc ttgagtgcta atggtgaggc aaaagtattc
     1681 agaccaaggg accgtgatga tattgtaaaa actgtgattg aaccgatggc atcagaaggc
     1741 ttgagaacca tatgtcttgc attcagagat tttccagcag gagaaccaga accagagtgg
     1801 gataatgaaa atgatattgt caccggcctt acatgcattg ctgttgtggg gattgaagat
     1861 cctgtgagac ctgaggtgcc agatgcaatt aaaaagtgtc agagggctgg aattactgtg
     1921 cggatggtca ctggtgataa tattaatact gctcgggcca ttgctaccaa atgtggtatt
     1981 ttacatcctg gggaagattt tctgtgccta gaaggtaaag attttaacag aagaatacga
     2041 aatgaaaaag gagagattga gcaagagagg atagacaaga tttggccaaa acttcgagta
     2101 cttgcaagat catctcctac tgataagcat acactggtta aaggtataat tgacagcact
     2161 gtctcagacc aacgccaggt tgtagctgta actggtgatg gtacaaatga tggcccagca
     2221 ctaaagaaag cagatgttgg atttgcaatg ggtattgctg gaactgatgt agctaaagaa
     2281 gcatccgata ttattctcac agatgacaac tttacaagca ttgttaaagc agttatgtgg
     2341 ggacgaaatg tctatgacag catctcaaaa ttccttcagt tccaacttac tgttaatgta
     2401 gtagcagtga ttgttgcttt tacgggcgcc tgcattactc aagactcacc gcttaaggct
     2461 gtgcagatgc tgtgggtaaa cctcataatg gatacactcg cttccctggc tctggcaacg
     2521 gaaccaccca ctgagtctct cttgcttcgg aaaccttatg gtagaaataa gcctctcatc
     2581 tcacgtacaa tgatgaagaa tattttgggt catgcattct atcaacttgt agtagtcttt
     2641 acactcttat ttgctggaga aaagtttttt gacattgata gtggaagaaa tgctcctttg
     2701 catgctcctc cttcagaaca ttatactatt gtttttaata cctttgtgct gatgcaactt
     2761 ttcaacgaaa taaatgcccg gaaaattcat ggtgaaagaa atgtattcga aggaatcttt
     2821 aacaatgcca tcttctgcac aattgtttta ggcacttttg tggtacagat aataattgtg
     2881 cagtttggtg gaaaaccttt cagttgttca gaactttcaa tagaacagtg gctatggtca
     2941 atattcctag gaatgggaac attactctgg ggccagctta tttcaacaat tccaactagc
     3001 cgtttaaaat tcctcaaaga agctggtcat ggaacacaaa aggaagaaat acctgaggag
     3061 gaattagcag aggatgttga agagattgat cacgctgaaa gggagttgcg gcgtggccaa
     3121 atcttgtggt ttagaggtct gaacagaatc caaacacaga ttcgagtggt gaatgcattt
     3181 cgtagttctt tatatgaagg gttagaaaaa ccggaatcaa gaagttcgat tcacaacttt
     3241 atgacacatc ctgagtttag gatagaagat tcagagcctc atatccccct tattgatgac
     3301 actgatgccg aagatgatgc tcctacaaaa cgtaactcca gtcctccacc ctctcccaac
     3361 aaaaataaca atgctgttga cagtggaatt caccttacaa tagaaatgaa caagtctgct
     3421 acctcttcat ccccaggaag cccactacat agtttggaaa catcactctg attgtaagct
     3481 gaatgttaac acactagctg cattgtaaag aaacaaattg aaactgggtc ttttcacata
     3541 ttgtgatgga caagctagta ttcttgtctt tggacttcaa cagaagacac acttgtacga
     3601 atgtagattt atttttttaa aaaaaaaaaa gcaaagcttt ctgccagact gagggtgctt
     3661 tttggggggt gggagaaatg aactgacaga taaacagtaa ctcagcgtaa gtgacctgtg
     3721 gatcatcagt agtacccaag catggtcctg aacagtgtat tagcagagct ttagtttatc
     3781 tcagtcctgg atgggagaga gagggaggag taaaactggg caagacaaag agccctggat
     3841 cgttgaattg atagtttaat ttctgctgtt ggctgttaat aatttggatt atttatgttt
     3901 ataaatgata cagatctgtt tacaaggttt gtagatactt tttttgttcc tgttcataga
     3961 tgggaagctt ccttataact gatgcagaga aaaattagtc cttcaaatac tgctgtattt
     4021 tcaggaaaaa aaaaaaaagg ggtgtttcta ttgaaactgt actaaatttt tgcctacaat
     4081 ttaccttgtt aaatattgta gataaattgc taatactaat agccaaatag ataacactaa
     4141 tgctttgttt aaaaaaacga aaaaaacaaa acaaaacaaa aaaacatgag cagtggtgtt
     4201 ctaatgaagt tatgacatct gtcctaatta gtttcttaaa tacatttact acttaatggt
     4261 tttgaagcaa ctgtttaatt tttaaaattt ttgaaaactt gctaataacc ttttgttcag
     4321 aatttagtag attgtttaat gcaggacatc aactacataa tgaaagatgc ttgaaatgct
     4381 tttgttattt caggcaaatc aaattaaatc agactatcaa actacaagag aaccttagtc
     4441 ttttttgttt ttgtctaaac atgttagttt agtgatgtct tggtgaactg tgagtgaact
     4501 gtattgattt ttctaatctt tagagacctt cacacagcat gagggctgtt ggcattttga
     4561 aaaaggttaa taagtagaag catacatgtt ttccttttgt ttttgaaact tgtttgtaaa
     4621 cataaataaa tatgcccttt tattaaataa attcatggca atgttttttt aaaagacatt
     4681 tcagcttctt tactcagttc tgcatataca gtgcatcagt ctgttctctt tgaccacaat
     4741 tggaatacat ttcaagatgg taacagcccc atgaagtacc gctttaattc cttgctggta
     4801 atctaataga atcagattaa gaaaattaac acaattttac catttgacat actcttcatt
     4861 gaatttagtt tgtctaaaat agtctcacag gtaaaaaaca taacaacaat gaccaccatt
     4921 gtctttgata catttatttt aattcttaag tggccaaata acagattagg taaaatgaga
     4981 ttacatgaaa aggttctaaa atgatatatt tagatgcaat aattaaccat atgctagaat
     5041 gcccacctct aagccaattg gattctttag ctctcttttt ctttccttca tctaccttct
     5101 tctctttttt gcctctgtct ttacccttta gaaactggca tgtgttaggg cccagatttc
     5161 accagataca caatcctcag gctgacataa agtttacgag gactgctact ataactgaga
     5221 gtgctgacca agcaaagaaa catttagttg atggtgcttg atatttttgg tgggtggggg
     5281 aaatcttttt ctcaccatca aatctttgca gtaatcctct gcaggtctta gaaacacatt
     5341 ggctctgtca gcatttgtaa ttgtacattt attcatcagg tatttatagt gaatcatact
     5401 gtaaaaaaga ggtagcatga tatgctggaa agcatattag actaggagtc aagacctggt
     5461 ttctagtctt ggttttgcca ttaatgtgct gcatgatctt gggaaaatca tttaccctaa
     5521 ataagggtgg tggactatct tgaagaaccc tttcaactct aaaaattgac cctatcaact
     5581 ggagagccca caaggcatag ggaatagaga aaagggaaat gttttcagtt ttctttttta
     5641 ttttggttat gcttaaatat gaatgtaatt accaaaagat ttagaagtgc atgtgctttg
     5701 gaggatttgt attgagcttt tacagtattc atttttcaac tcaaggcaat ggctttctac
     5761 accaactcta atccataaac gggtcttatg acatctatga agtagtagca agacatgctt
     5821 agtgtgtatt tctctctttg agacactgta atttctacca gaaatttcca gagcattatg
     5881 taggtagaaa aaaatgcaag caagctgtta aagatcttgg atcccattat atagtatgta
     5941 tagctgaaat ctgtaattca atcacttttt ctcttttatc ctctaaccaa aaaattgttt
     6001 aattttgcat cccaaatgtt tttaatcttt gtatattttt taaaaatcct tttctcctca
     6061 tcattgcctt ttttgtggtt gtaaatagac ttacttgcac tttgaagatg agttactcct
     6121 tgtcatctta caaatatgtg atatggtaat tttcataaca gatgtcagtt ttgaaccaag
     6181 aattggtgat ttgtttataa gaaaaaaact ggcttcattt ctgtgaaatt gctctttgaa
     6241 aatttctttt tacacgtgta agccaactga gataccgtga tggtgttgat ttctttcaat
     6301 gatgcttacc atctatttta gccactgagc cttttattat ttgtctattt gtaaagttta
     6361 tttgtcttaa ctcatttaat aaatatactg tttatctgtt tctgaa
//
