LOCUS       NM_001366520            7162 bp    mRNA    linear   PRI 02-MAY-2024
DEFINITION  Homo sapiens ATPase plasma membrane Ca2+ transporting 1 (ATP2B1),
            transcript variant 3, mRNA.
ACCESSION   NM_001366520
VERSION     NM_001366520.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Kobayashi,Y., Yatsu,K., Haruna,A., Kawano,R., Ozawa,M., Haze,T.,
            Komiya,S., Suzuki,S., Ohki,Y., Fujiwara,A., Saka,S., Hirawa,N.,
            Toya,Y. and Tamura,K.
  TITLE     ATP2B1 gene polymorphisms associated with resistant hypertension in
            the Japanese population
  JOURNAL   J Clin Hypertens (Greenwich) 26 (4), 355-362 (2024)
   PUBMED   38430457
  REMARK    GeneRIF: ATP2B1 gene polymorphisms associated with resistant
            hypertension in the Japanese population.
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Gao,Y., Liu,Y., Jiang,Y., Qin,M., Guan,Z., Gao,Y. and Yang,Y.
  TITLE     Association between polymorphism and haplotype of ATP2B1 gene and
            skeletal fluorosis in Han population
  JOURNAL   Int J Environ Health Res 34 (3), 1410-1420 (2024)
   PUBMED   37211801
  REMARK    GeneRIF: Association between polymorphism and haplotype of ATP2B1
            gene and skeletal fluorosis in Han population.
REFERENCE   3  (bases 1 to 7162)
  AUTHORS   Berrocal,M. and Mata,A.M.
  TITLE     The Plasma Membrane Ca2+-ATPase, a Molecular Target for Tau-induced
            Cytosolic Calcium Dysregulation
  JOURNAL   Neuroscience 518, 112-118 (2023)
   PUBMED   35469971
  REMARK    GeneRIF: The Plasma Membrane Ca[2+]-ATPase, a Molecular Target for
            Tau-induced Cytosolic Calcium Dysregulation.
            Review article
REFERENCE   4  (bases 1 to 7162)
  AUTHORS   Yang,X., Lu,L., Wu,C. and Zhang,F.
  TITLE     ATP2B1-AS1 exacerbates sepsis-induced cell apoptosis and
            inflammation by regulating miR-23a-3p/TLR4 axis
  JOURNAL   Allergol Immunopathol (Madr) 51 (2), 17-26 (2023)
   PUBMED   36916084
  REMARK    GeneRIF: ATP2B1-AS1 exacerbates sepsis-induced cell apoptosis and
            inflammation by regulating miR-23a-3p/TLR4 axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7162)
  AUTHORS   Rahimi,M.J., Urban,N., Wegler,M., Sticht,H., Schaefer,M., Popp,B.,
            Gaunitz,F., Morleo,M., Nigro,V., Maitz,S., Mancini,G.M.S.,
            Ruivenkamp,C., Suk,E.K., Bartolomaeus,T., Merkenschlager,A.,
            Koboldt,D., Bartholomew,D., Stegmann,A.P.A., Sinnema,M.,
            Duynisveld,I., Salvarinova,R., Race,S., de Vries,B.B.A.,
            Trimouille,A., Naudion,S., Marom,D., Hamiel,U., Henig,N.,
            Demurger,F., Rahner,N., Bartels,E., Hamm,J.A., Putnam,A.M.,
            Person,R., Abou Jamra,R. and Oppermann,H.
  TITLE     De novo variants in ATP2B1 lead to neurodevelopmental delay
  JOURNAL   Am J Hum Genet 109 (5), 944-952 (2022)
   PUBMED   35358416
  REMARK    GeneRIF: De novo variants in ATP2B1 lead to neurodevelopmental
            delay.
REFERENCE   6  (bases 1 to 7162)
  AUTHORS   Kessler,F., Falchetto,R., Heim,R., Meili,R., Vorherr,T.,
            Strehler,E.E. and Carafoli,E.
  TITLE     Study of calmodulin binding to the alternatively spliced C-terminal
            domain of the plasma membrane Ca2+ pump
  JOURNAL   Biochemistry 31 (47), 11785-11792 (1992)
   PUBMED   1332771
REFERENCE   7  (bases 1 to 7162)
  AUTHORS   Wang,K.K., Wright,L.C., Machan,C.L., Allen,B.G., Conigrave,A.D. and
            Roufogalis,B.D.
  TITLE     Protein kinase C phosphorylates the carboxyl terminus of the plasma
            membrane Ca(2+)-ATPase from human erythrocytes
  JOURNAL   J Biol Chem 266 (14), 9078-9085 (1991)
   PUBMED   1827443
REFERENCE   8  (bases 1 to 7162)
  AUTHORS   Olson,S., Wang,M.G., Carafoli,E., Strehler,E.E. and McBride,O.W.
  TITLE     Localization of two genes encoding plasma membrane
            Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and
            12q21-23
  JOURNAL   Genomics 9 (4), 629-641 (1991)
   PUBMED   1674727
REFERENCE   9  (bases 1 to 7162)
  AUTHORS   Strehler,E.E., Strehler-Page,M.A., Vogel,G. and Carafoli,E.
  TITLE     mRNAs for plasma membrane calcium pump isoforms differing in their
            regulatory domain are generated by alternative splicing that
            involves two internal donor sites in a single exon
  JOURNAL   Proc Natl Acad Sci U S A 86 (18), 6908-6912 (1989)
   PUBMED   2528729
REFERENCE   10 (bases 1 to 7162)
  AUTHORS   James,P.H., Pruschy,M., Vorherr,T.E., Penniston,J.T. and
            Carafoli,E.
  TITLE     Primary structure of the cAMP-dependent phosphorylation site of the
            plasma membrane calcium pump
  JOURNAL   Biochemistry 28 (10), 4253-4258 (1989)
   PUBMED   2548572
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC009522.11, AC068641.18 and
            AC025034.22.
            
            Summary: The protein encoded by this gene belongs to the family of
            P-type primary ion transport ATPases characterized by the formation
            of an aspartyl phosphate intermediate during the reaction cycle.
            These enzymes remove bivalent calcium ions from eukaryotic cells
            against very large concentration gradients and play a critical role
            in intracellular calcium homeostasis. The mammalian plasma membrane
            calcium ATPase isoforms are encoded by at least four separate genes
            and the diversity of these enzymes is further increased by
            alternative splicing of transcripts. The expression of different
            isoforms and splice variants is regulated in a developmental,
            tissue- and cell type-specific manner, suggesting that these pumps
            are functionally adapted to the physiological needs of particular
            cells and tissues. This gene encodes the plasma membrane calcium
            ATPase isoform 1. Alternatively spliced transcript variants
            encoding different isoforms have been identified. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (3), as well as variants 2, 4, 5,
            16, and 17, encodes isoform 1b.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.946060.1,
                                           SRR18074967.1377360.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-148               AC009522.11        112886-113033
            149-343             AC068641.18        17774-17968
            344-772             AC068641.18        39483-39911
            773-970             AC068641.18        53235-53432
            971-1225            AC068641.18        60339-60593
            1226-1351           AC068641.18        60687-60812
            1352-1492           AC068641.18        64945-65085
            1493-1531           AC068641.18        67874-67912
            1532-1693           AC068641.18        68975-69136
            1694-1908           AC068641.18        71193-71407
            1909-2151           AC068641.18        73799-74041
            2152-2393           AC068641.18        75350-75591
            2394-2631           AC068641.18        78551-78788
            2632-2811           AC068641.18        84218-84397
            2812-2899           AC068641.18        85082-85169
            2900-3006           AC068641.18        85547-85653
            3007-3198           AC068641.18        91244-91435
            3199-3412           AC068641.18        91665-91878
            3413-3624           AC068641.18        92336-92547
            3625-3732           AC068641.18        94157-94264
            3733-3915           AC068641.18        96291-96473
            3916-4142           AC068641.18        104295-104521
            4143-7162           AC025034.22        1-3020
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q21.33"
     gene            1..7162
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="ATPase plasma membrane Ca2+ transporting 1"
                     /db_xref="GeneID:490"
                     /db_xref="HGNC:HGNC:814"
                     /db_xref="MIM:108731"
     exon            1..148
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            149..343
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            344..772
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     CDS             565..4227
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /EC_number="7.2.2.10"
                     /note="isoform 1b is encoded by transcript variant 3;
                     plasma membrane calcium pump; ATPase, Ca++ transporting,
                     plasma membrane 1; plasma membrane calcium-transporting
                     ATPase 1"
                     /codon_start=1
                     /product="plasma membrane calcium-transporting ATPase 1
                     isoform 1b"
                     /protein_id="NP_001353449.1"
                     /db_xref="CCDS:CCDS9035.1"
                     /db_xref="GeneID:490"
                     /db_xref="HGNC:HGNC:814"
                     /db_xref="MIM:108731"
                     /translation="MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTD
                     ALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLV
                     WEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAIL
                     LSVVCVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQV
                     KYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVT
                     AVGVNSQTGIIFTLLGAGGEEEEKKDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQ
                     PLKSEEGGDGDEKDKKKANLPKKEKSVLQGKLTKLAVQIGKAGLLMSAITVIILVLYF
                     VIDTFWVQKRPWLAECTPIYIQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKM
                     MKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYINEKHYKKVPEPEAIPP
                     NILSYLVTGISVNCAYTSKILPPEKEGGLPRHVGNKTECALLGLLLDLKRDYQDVRNE
                     IPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFSKGASEIILKKCFKILSANGEAKVF
                     RPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEPEPEWDNENDIVTGLTCIAVVGI
                     EDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIATKCGILHPGEDFLCLEGKDFN
                     RRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVSDQRQVVAVTGDG
                     TNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFL
                     QFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASLALATEPPTESLLLR
                     KPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGRNAPLHAPPSEHY
                     TIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQIIIVQFGGKP
                     FSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEEIPEEELAE
                     DVEEIDHAERELRRGQILWFRGLNRIQTQIRVVNAFRSSLYEGLEKPESRSSIHNFMT
                     HPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEMNKSA
                     TSSSPGSPLHSLETSL"
     exon            773..970
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1225
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1351
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1492
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1531
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1693
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1908
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            1909..2151
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2152..2393
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2631
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2632..2811
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2812..2899
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            2900..3006
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3007..3198
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3412
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3413..3624
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3625..3732
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3915
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     exon            3916..7162
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5381..5386
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="hexamer: AATAAA"
     polyA_site      5407
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="major polyA site"
     regulatory      7134..7139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="hexamer: AATAAA"
     regulatory      7138..7143
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="hexamer: AATATA"
     polyA_site      7156
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
     polyA_site      7162
                     /gene="ATP2B1"
                     /gene_synonym="MRD66; PMCA1; PMCA1kb"
                     /note="major polyA site"
ORIGIN      
        1 gtcacgtgac cgcgctcagc caaggcggca gcggcggcgg cgcgagcttc ggcggcggtg
       61 gcggctgtgg cagcgggagc agctgcgccg ccagcgtgga gcgggaggcg tgtgggtgtg
      121 gagaaaaggg agcgccagac cggaacgggt ttttgattaa agagtgaaaa ggatgaacat
      181 ataatttaat tggtattcta ctactggtgg aattctgaat agtcacaccc gattttatga
      241 tactgtctaa gaagaagaaa agaacattgg tatgaagttt ggtatcagga gaaataagca
      301 aatgaaaaga gttgaaggaa ccagtttatg tatttttatg gaaatgtgta tatctcatga
      361 ttgatatgga gaaactagtc atgggccaaa ggtcaagata cttctctggg aaatgttgct
      421 gctgatgctg ctttacaaag tcatacaatg agtgtttggt ttaagaaaga ttttcatact
      481 taaaagattt tcatcttgga aatacatcaa gtgaaaatta aattcttttg ggaaacattt
      541 tccttctgat atattatact tgtaatgggc gacatggcaa acaactcagt tgcttacagt
      601 ggtgtgaaaa actctttgaa ggaagctaat catgatggag actttggaat tacgctcgca
      661 gagctgcggg ctctcatgga gctcaggtcc acagatgcat tacgaaaaat acaggaaagc
      721 tatggagatg tctatggaat ttgcaccaaa ttgaaaacat ctcccaatga aggtttaagt
      781 ggaaaccctg cagatttaga aagaagagaa gcagtgtttg gaaagaattt tatacctcct
      841 aaaaagccaa aaacctttct tcaattagta tgggaagcat tacaagatgt cactttaatt
      901 atattagaaa ttgcagccat agtatcattg ggcctttctt tttatcagcc tccagaaggg
      961 gataatgcac tttgtggaga agtttctgtt ggggaggaag aaggtgaagg tgaaactggt
     1021 tggattgaag gagctgcaat cctcttgtct gtagtgtgtg tggtgttagt aacagctttc
     1081 aatgactgga gtaaggaaaa acagtttaga ggtttgcaga gccgaattga acaagaacag
     1141 aagttcactg tcatcagggg tggtcaggtc attcagatac ctgtagctga cattactgtt
     1201 ggagatattg ctcaagtgaa atatggtgat cttcttccag ctgacggcat acttattcaa
     1261 ggcaacgatc ttaaaattga tgaaagctca ttgactggtg aatcagatca tgttaaaaag
     1321 tctttagata aggatccctt acttctatca ggtactcatg taatggaagg ctctggaaga
     1381 atggtagtta cagctgtagg tgtaaattct caaactggaa ttatctttac cttacttgga
     1441 gctggaggtg aagaggaaga gaagaaagat gagaagaaaa aggaaaagaa aaataagaaa
     1501 caagatggag ctattgagaa tcgcaacaaa gcaaaagccc aggatggtgc agccatggaa
     1561 atgcagccat tgaagagtga agaaggtgga gatggtgatg aaaaagataa aaagaaagca
     1621 aatttgccaa aaaaggaaaa atctgtttta caagggaaac ttacaaaact ggctgttcag
     1681 attggcaaag caggtctgtt gatgtctgcc atcacagtta tcattctagt attatatttt
     1741 gtcattgaca ccttctgggt tcagaaaaga ccatggcttg ctgagtgcac accaatttat
     1801 atacaatact ttgtgaagtt cttcattatt ggagttacag ttttagtggt cgcagtgcca
     1861 gaaggtcttc cacttgcagt cacgatctca ctggcttatt cagtcaaaaa aatgatgaaa
     1921 gataataact tagtaaggca tctggatgct tgtgaaacca tgggaaatgc tacagctatt
     1981 tgttcagata aaacaggaac tttgacaatg aacagaatga cagtcgttca agcttacata
     2041 aatgaaaaac attataaaaa ggttcctgaa ccagaagcta ttccaccaaa tattttgtcc
     2101 tatcttgtaa caggaatttc tgtgaattgt gcttatacat caaaaatatt gccaccagag
     2161 aaagagggtg gattacctcg tcacgttggt aataaaactg aatgtgcctt gttgggactt
     2221 cttttggatt taaaacggga ttatcaggat gttagaaatg aaataccaga agaagcactg
     2281 tacaaagtct acaccttcaa ttctgttagg aagtccatga gtactgtcct gaaaaattca
     2341 gatggaagtt atcgaatatt cagcaagggt gcatctgaga taattctgaa aaagtgtttc
     2401 aaaatcttga gtgctaatgg tgaggcaaaa gtattcagac caagggaccg tgatgatatt
     2461 gtaaaaactg tgattgaacc gatggcatca gaaggcttga gaaccatatg tcttgcattc
     2521 agagattttc cagcaggaga accagaacca gagtgggata atgaaaatga tattgtcacc
     2581 ggccttacat gcattgctgt tgtggggatt gaagatcctg tgagacctga ggtgccagat
     2641 gcaattaaaa agtgtcagag ggctggaatt actgtgcgga tggtcactgg tgataatatt
     2701 aatactgctc gggccattgc taccaaatgt ggtattttac atcctgggga agattttctg
     2761 tgcctagaag gtaaagattt taacagaaga atacgaaatg aaaaaggaga gattgagcaa
     2821 gagaggatag acaagatttg gccaaaactt cgagtacttg caagatcatc tcctactgat
     2881 aagcatacac tggttaaagg tataattgac agcactgtct cagaccaacg ccaggttgta
     2941 gctgtaactg gtgatggtac aaatgatggc ccagcactaa agaaagcaga tgttggattt
     3001 gcaatgggta ttgctggaac tgatgtagct aaagaagcat ccgatattat tctcacagat
     3061 gacaacttta caagcattgt taaagcagtt atgtggggac gaaatgtcta tgacagcatc
     3121 tcaaaattcc ttcagttcca acttactgtt aatgtagtag cagtgattgt tgcttttacg
     3181 ggcgcctgca ttactcaaga ctcaccgctt aaggctgtgc agatgctgtg ggtaaacctc
     3241 ataatggata cactcgcttc cctggctctg gcaacggaac cacccactga gtctctcttg
     3301 cttcggaaac cttatggtag aaataagcct ctcatctcac gtacaatgat gaagaatatt
     3361 ttgggtcatg cattctatca acttgtagta gtctttacac tcttatttgc tggagaaaag
     3421 ttttttgaca ttgatagtgg aagaaatgct cctttgcatg ctcctccttc agaacattat
     3481 actattgttt ttaatacctt tgtgctgatg caacttttca acgaaataaa tgcccggaaa
     3541 attcatggtg aaagaaatgt attcgaagga atctttaaca atgccatctt ctgcacaatt
     3601 gttttaggca cttttgtggt acagataata attgtgcagt ttggtggaaa acctttcagt
     3661 tgttcagaac tttcaataga acagtggcta tggtcaatat tcctaggaat gggaacatta
     3721 ctctggggcc agcttatttc aacaattcca actagccgtt taaaattcct caaagaagct
     3781 ggtcatggaa cacaaaagga agaaatacct gaggaggaat tagcagagga tgttgaagag
     3841 attgatcacg ctgaaaggga gttgcggcgt ggccaaatct tgtggtttag aggtctgaac
     3901 agaatccaaa cacagattcg agtggtgaat gcatttcgta gttctttata tgaagggtta
     3961 gaaaaaccgg aatcaagaag ttcgattcac aactttatga cacatcctga gtttaggata
     4021 gaagattcag agcctcatat cccccttatt gatgacactg atgccgaaga tgatgctcct
     4081 acaaaacgta actccagtcc tccaccctct cccaacaaaa ataacaatgc tgttgacagt
     4141 ggaattcacc ttacaataga aatgaacaag tctgctacct cttcatcccc aggaagccca
     4201 ctacatagtt tggaaacatc actctgattg taagctgaat gttaacacac tagctgcatt
     4261 gtaaagaaac aaattgaaac tgggtctttt cacatattgt gatggacaag ctagtattct
     4321 tgtctttgga cttcaacaga agacacactt gtacgaatgt agatttattt ttttaaaaaa
     4381 aaaaaagcaa agctttctgc cagactgagg gtgctttttg gggggtggga gaaatgaact
     4441 gacagataaa cagtaactca gcgtaagtga cctgtggatc atcagtagta cccaagcatg
     4501 gtcctgaaca gtgtattagc agagctttag tttatctcag tcctggatgg gagagagagg
     4561 gaggagtaaa actgggcaag acaaagagcc ctggatcgtt gaattgatag tttaatttct
     4621 gctgttggct gttaataatt tggattattt atgtttataa atgatacaga tctgtttaca
     4681 aggtttgtag atactttttt tgttcctgtt catagatggg aagcttcctt ataactgatg
     4741 cagagaaaaa ttagtccttc aaatactgct gtattttcag gaaaaaaaaa aaaaggggtg
     4801 tttctattga aactgtacta aatttttgcc tacaatttac cttgttaaat attgtagata
     4861 aattgctaat actaatagcc aaatagataa cactaatgct ttgtttaaaa aaacgaaaaa
     4921 aacaaaacaa aacaaaaaaa catgagcagt ggtgttctaa tgaagttatg acatctgtcc
     4981 taattagttt cttaaataca tttactactt aatggttttg aagcaactgt ttaattttta
     5041 aaatttttga aaacttgcta ataacctttt gttcagaatt tagtagattg tttaatgcag
     5101 gacatcaact acataatgaa agatgcttga aatgcttttg ttatttcagg caaatcaaat
     5161 taaatcagac tatcaaacta caagagaacc ttagtctttt ttgtttttgt ctaaacatgt
     5221 tagtttagtg atgtcttggt gaactgtgag tgaactgtat tgatttttct aatctttaga
     5281 gaccttcaca cagcatgagg gctgttggca ttttgaaaaa ggttaataag tagaagcata
     5341 catgttttcc ttttgttttt gaaacttgtt tgtaaacata aataaatatg cccttttatt
     5401 aaataaattc atggcaatgt ttttttaaaa gacatttcag cttctttact cagttctgca
     5461 tatacagtgc atcagtctgt tctctttgac cacaattgga atacatttca agatggtaac
     5521 agccccatga agtaccgctt taattccttg ctggtaatct aatagaatca gattaagaaa
     5581 attaacacaa ttttaccatt tgacatactc ttcattgaat ttagtttgtc taaaatagtc
     5641 tcacaggtaa aaaacataac aacaatgacc accattgtct ttgatacatt tattttaatt
     5701 cttaagtggc caaataacag attaggtaaa atgagattac atgaaaaggt tctaaaatga
     5761 tatatttaga tgcaataatt aaccatatgc tagaatgccc acctctaagc caattggatt
     5821 ctttagctct ctttttcttt ccttcatcta ccttcttctc ttttttgcct ctgtctttac
     5881 cctttagaaa ctggcatgtg ttagggccca gatttcacca gatacacaat cctcaggctg
     5941 acataaagtt tacgaggact gctactataa ctgagagtgc tgaccaagca aagaaacatt
     6001 tagttgatgg tgcttgatat ttttggtggg tgggggaaat ctttttctca ccatcaaatc
     6061 tttgcagtaa tcctctgcag gtcttagaaa cacattggct ctgtcagcat ttgtaattgt
     6121 acatttattc atcaggtatt tatagtgaat catactgtaa aaaagaggta gcatgatatg
     6181 ctggaaagca tattagacta ggagtcaaga cctggtttct agtcttggtt ttgccattaa
     6241 tgtgctgcat gatcttggga aaatcattta ccctaaataa gggtggtgga ctatcttgaa
     6301 gaaccctttc aactctaaaa attgacccta tcaactggag agcccacaag gcatagggaa
     6361 tagagaaaag ggaaatgttt tcagttttct tttttatttt ggttatgctt aaatatgaat
     6421 gtaattacca aaagatttag aagtgcatgt gctttggagg atttgtattg agcttttaca
     6481 gtattcattt ttcaactcaa ggcaatggct ttctacacca actctaatcc ataaacgggt
     6541 cttatgacat ctatgaagta gtagcaagac atgcttagtg tgtatttctc tctttgagac
     6601 actgtaattt ctaccagaaa tttccagagc attatgtagg tagaaaaaaa tgcaagcaag
     6661 ctgttaaaga tcttggatcc cattatatag tatgtatagc tgaaatctgt aattcaatca
     6721 ctttttctct tttatcctct aaccaaaaaa ttgtttaatt ttgcatccca aatgttttta
     6781 atctttgtat attttttaaa aatccttttc tcctcatcat tgcctttttt gtggttgtaa
     6841 atagacttac ttgcactttg aagatgagtt actccttgtc atcttacaaa tatgtgatat
     6901 ggtaattttc ataacagatg tcagttttga accaagaatt ggtgatttgt ttataagaaa
     6961 aaaactggct tcatttctgt gaaattgctc tttgaaaatt tctttttaca cgtgtaagcc
     7021 aactgagata ccgtgatggt gttgatttct ttcaatgatg cttaccatct attttagcca
     7081 ctgagccttt tattatttgt ctatttgtaa agtttatttg tcttaactca tttaataaat
     7141 atactgttta tctgtttctg aa
//
