LOCUS       NM_001349232            5333 bp    mRNA    linear   PRI 07-DEC-2024
DEFINITION  Homo sapiens autophagy related 7 (ATG7), transcript variant 4,
            mRNA.
ACCESSION   NM_001349232 XM_011533282
VERSION     NM_001349232.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Qin,J., Chen,Y., Zhao,X. and Yu,J.
  TITLE     circCUL3 drives malignant progression of cervical cancer by
            activating autophagy through sponge miR-223-3p upregulation of ATG7
  JOURNAL   Gene 925, 148572 (2024)
   PUBMED   38759738
  REMARK    GeneRIF: circCUL3 drives malignant progression of cervical cancer
            by activating autophagy through sponge miR-223-3p upregulation of
            ATG7.
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Ostacolo,K., Lopez Garcia de Lomana,A., Larat,C., Hjaltalin,V.,
            Holm,K.Y., Hlynsdottir,S.S., Soucheray,M., Sooman,L., Rolfsson,O.,
            Krogan,N.J., Steingrimsson,E., Swaney,D.L. and Ogmundsdottir,M.H.
  TITLE     ATG7(2) Interacts With Metabolic Proteins and Regulates Central
            Energy Metabolism
  JOURNAL   Traffic 25 (4), e12933 (2024)
   PUBMED   38600522
  REMARK    GeneRIF: ATG7(2) Interacts With Metabolic Proteins and Regulates
            Central Energy Metabolism.
REFERENCE   3  (bases 1 to 5333)
  AUTHORS   Liu,Q., Han,Y., Zhang,M., Yang,P., Xiang,Y., Chen,M., Xu,F.,
            Zhou,X., Zheng,D. and Qin,J.
  TITLE     IGF1R stimulates autophagy, enhances viability, and promotes
            insulin secretion in pancreatic beta cells in gestational diabetes
            mellitus by upregulating ATG7
  JOURNAL   Reprod Biol 24 (1), 100850 (2024)
   PUBMED   38262267
  REMARK    GeneRIF: IGF1R stimulates autophagy, enhances viability, and
            promotes insulin secretion in pancreatic beta cells in gestational
            diabetes mellitus by upregulating ATG7.
REFERENCE   4  (bases 1 to 5333)
  AUTHORS   Meng,Y., Bian,L., Zhang,M., Zhou,P., Zhang,S., Ying,Y., Yang,S.,
            Liu,Y., Yao,Y. and Li,D.
  TITLE     ISG15 Promotes Progression and Gemcitabine Resistance of Pancreatic
            Cancer Cells Through ATG7
  JOURNAL   Int J Biol Sci 20 (4), 1180-1193 (2024)
   PUBMED   38385083
  REMARK    GeneRIF: ISG15 Promotes Progression and Gemcitabine Resistance of
            Pancreatic Cancer Cells Through ATG7.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5333)
  AUTHORS   Liu,J., Lu,S., Zheng,L., Guo,Q., Cao,L., Xiao,Y., Chen,D., Zou,Y.,
            Liu,X., Deng,C., Zhang,S., Yang,R., Wang,Y., Zhang,Y., Zhang,N.,
            Song,X., Xing,C., Wang,Z. and Cao,L.
  TITLE     ATM-CHK2-TRIM32 axis regulates ATG7 ubiquitination to initiate
            autophagy under oxidative stress
  JOURNAL   Cell Rep 42 (11), 113402 (2023)
   PUBMED   37943659
REFERENCE   6  (bases 1 to 5333)
  AUTHORS   Nemoto,T., Tanida,I., Tanida-Miyake,E., Minematsu-Ikeguchi,N.,
            Yokota,M., Ohsumi,M., Ueno,T. and Kominami,E.
  TITLE     The mouse APG10 homologue, an E2-like enzyme for Apg12p
            conjugation, facilitates MAP-LC3 modification
  JOURNAL   J Biol Chem 278 (41), 39517-39526 (2003)
   PUBMED   12890687
REFERENCE   7  (bases 1 to 5333)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Komatsu,M., Ueno,T. and Kominami,E.
  TITLE     Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple
            substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the
            conjugation of hApg12p to hApg5p
  JOURNAL   J Biol Chem 277 (16), 13739-13744 (2002)
   PUBMED   11825910
REFERENCE   8  (bases 1 to 5333)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Nishitani,T., Komatsu,M., Yamazaki,H.,
            Ueno,T. and Kominami,E.
  TITLE     Murine Apg12p has a substrate preference for murine Apg7p over
            three Apg8p homologs
  JOURNAL   Biochem Biophys Res Commun 292 (1), 256-262 (2002)
   PUBMED   11890701
REFERENCE   9  (bases 1 to 5333)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Ueno,T. and Kominami,E.
  TITLE     The human homolog of Saccharomyces cerevisiae Apg7p is a
            Protein-activating enzyme for multiple substrates including human
            Apg12p, GATE-16, GABARAP, and MAP-LC3
  JOURNAL   J Biol Chem 276 (3), 1701-1706 (2001)
   PUBMED   11096062
REFERENCE   10 (bases 1 to 5333)
  AUTHORS   Yuan,W., Stromhaug,P.E. and Dunn,W.A. Jr.
  TITLE     Glucose-induced autophagy of peroxisomes in Pichia pastoris
            requires a unique E1-like protein
  JOURNAL   Mol Biol Cell 10 (5), 1353-1366 (1999)
   PUBMED   10233149
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC083855.3, AC020750.4,
            AC026185.4 and AC022001.5.
            
            On Jun 2, 2019 this sequence version replaced NM_001349232.1.
            
            Summary: This gene encodes an E1-like activating enzyme that is
            essential for autophagy and cytoplasmic to vacuole transport. The
            encoded protein is also thought to modulate p53-dependent cell
            cycle pathways during prolonged metabolic stress. It has been
            associated with multiple functions, including axon membrane
            trafficking, axonal homeostasis, mitophagy, adipose
            differentiation, and hematopoietic stem cell maintenance.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (4) represents the longest
            transcript and encodes the longest isoform (a). Variants 1, 4, 5,
            6, and 7 all encode the same isoform (a).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.83723.1,
                                           SRR1803615.122845.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000693202.1/ ENSP00000510336.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                AC083855.3         170608-170641
            35-143              AC083855.3         179205-179313
            144-389             AC083855.3         180404-180649
            390-559             AC020750.4         86915-87084         c
            560-614             AC020750.4         86354-86408         c
            615-732             AC020750.4         78710-78827         c
            733-810             AC020750.4         76709-76786         c
            811-927             AC020750.4         72350-72466         c
            928-1077            AC020750.4         70277-70426         c
            1078-1166           AC020750.4         54342-54430         c
            1167-1288           AC020750.4         52677-52798         c
            1289-1379           AC020750.4         45035-45125         c
            1380-1524           AC020750.4         43491-43635         c
            1525-1683           AC020750.4         37735-37893         c
            1684-1878           AC020750.4         27158-27352         c
            1879-2082           AC020750.4         24986-25189         c
            2083-2198           AC020750.4         22842-22957         c
            2199-2274           AC020750.4         21036-21111         c
            2275-2355           AC020750.4         5718-5798           c
            2356-2478           AC026185.4         88899-89021         c
            2479-5333           AC022001.5         114612-117466       c
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..5333
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /note="autophagy related 7"
                     /db_xref="GeneID:10533"
                     /db_xref="HGNC:HGNC:16935"
                     /db_xref="MIM:608760"
     exon            1..34
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            35..143
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            144..389
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            390..559
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     CDS             400..2511
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /note="isoform a is encoded by transcript variant 4;
                     ubiquitin-like modifier-activating enzyme ATG7;
                     ATG12-activating enzyme E1 ATG7; hAGP7;
                     ubiquitin-activating enzyme E1-like protein; APG7
                     autophagy 7-like"
                     /codon_start=1
                     /product="ubiquitin-like modifier-activating enzyme ATG7
                     isoform a"
                     /protein_id="NP_001336161.1"
                     /db_xref="CCDS:CCDS2605.1"
                     /db_xref="GeneID:10533"
                     /db_xref="HGNC:HGNC:16935"
                     /db_xref="MIM:608760"
                     /translation="MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAP
                     KDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADKK
                     LLLEQAANEIWESIKSGTALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLP
                     LIQGPVGLDQRFSLKQIEALECAYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSD
                     FFQGQRTKITIGVYDPCNLAQYPGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGA
                     RDVAHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVD
                     LNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISY
                     SNPVRQPLYEFEDCLGGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVT
                     LEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVM
                     RHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDR
                     TLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPH
                     QIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGL
                     TLLHQETQAAEIWDMSDDETI"
     exon            560..614
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            615..732
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            733..810
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            811..927
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1077
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1078..1166
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1288
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1379
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1524
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1683
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1878
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2082
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            2083..2198
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2274
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            2275..2355
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            2356..2478
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..5333
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2713..2718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /note="hexamer: AATAAT"
     polyA_site      2751
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /note="major polyA site"
     regulatory      5312..5317
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
                     /note="hexamer: AATAAA"
     polyA_site      5333
                     /gene="ATG7"
                     /gene_synonym="APG7-LIKE; APG7L; GSA7; SCAR31"
ORIGIN      
        1 agagagagct gtggttgccg gaagttgagc ggcggatcca attcctgtat cttcaccttg
       61 tctaccggga tttctagagc agccttgtga gagacatttc tgagacctgt atgtcctgcg
      121 tctatgattc ttccctgatc aaggcttctc aaaccccata atgctggtat ccagtgcatc
      181 cgttctcaaa aactgaagct gatggacacc agattgcatg tgaaggtgaa ggatattata
      241 gcagaaggaa accaaaataa aagaaaaaca gctcctggaa gtaaccaatg agaaggacag
      301 ataagagttt gaagactgtg gatgtgactg cttgttccct ggttgacaca ctgaagaaga
      361 aatcatgttg attcagagtt ggcttggagg caagaaataa tggcggcagc tacgggggat
      421 cctggactct ctaaactgca gtttgcccct tttagtagtg ccttggatgt tgggttttgg
      481 catgagttga cccagaagaa gctgaacgag tatcggctgg atgaagctcc caaggacatt
      541 aagggttatt actacaatgg tgactctgct gggctgccag ctcgcttaac attggagttc
      601 agtgcttttg acatgagtgc tcccacccca gcccgttgct gcccagctat tggaacactg
      661 tataacacca acacactcga gtctttcaag actgcagata agaagctcct tttggaacaa
      721 gcagcaaatg agatatggga atccataaaa tcaggcactg ctcttgaaaa ccctgtactc
      781 ctcaacaagt tcctcctctt gacatttgca gatctaaaga agtaccactt ctactattgg
      841 ttttgctatc ctgccctctg tcttccagag agtttacctc tcattcaggg gccagtgggt
      901 ttggatcaaa ggttttcact aaaacagatt gaagcactag agtgtgcata tgataatctt
      961 tgtcaaacag aaggagtcac agctcttcct tacttcttaa tcaagtatga tgagaacatg
     1021 gtgctggttt ccttgcttaa acactacagt gatttcttcc aaggtcaaag gacgaagata
     1081 acaattggtg tatatgatcc ctgtaactta gcccagtacc ctggatggcc tttgaggaat
     1141 tttttggtcc tagcagccca cagatggagt agcagtttcc agtctgttga agttgtttgc
     1201 ttccgtgacc gtaccatgca gggggcgaga gacgttgccc acagcatcat cttcgaagtg
     1261 aagcttccag aaatggcatt tagcccagat tgtcctaaag cagttggatg ggaaaagaac
     1321 cagaaaggag gcatgggacc aaggatggtg aacctcagtg aatgtatgga ccctaaaagg
     1381 ttagctgagt catcagtgga tctaaatctc aaactgatgt gttggagatt ggttcctact
     1441 ttagacttgg acaaggttgt gtctgtcaaa tgtctgctgc ttggagccgg caccttgggt
     1501 tgcaatgtag ctaggacgtt gatgggttgg ggcgtgagac acatcacatt tgtggacaat
     1561 gccaagatct cctactccaa tcctgtgagg cagcctctct atgagtttga agattgccta
     1621 gggggtggta agcccaaggc tctggcagca gcggaccggc tccagaaaat attccccggt
     1681 gtgaatgcca gaggattcaa catgagcata cctatgcctg ggcatccagt gaacttctcc
     1741 agtgtcactc tggagcaagc ccgcagagat gtggagcaac tggagcagct catcgaaagc
     1801 catgatgtcg tcttcctatt gatggacacc agggagagcc ggtggcttcc tgccgtcatt
     1861 gctgcaagca agagaaagct ggtcatcaat gctgctttgg gatttgacac atttgttgtc
     1921 atgagacatg gtctgaagaa accaaagcag caaggagctg gggacttgtg tccaaaccac
     1981 cctgtggcat ctgctgacct cctgggctca tcgctttttg ccaacatccc tggttacaag
     2041 cttggctgct acttctgcaa tgatgtggtg gccccaggag attcaaccag agaccggacc
     2101 ttggaccagc agtgcactgt gagtcgtcca ggactggccg tgattgcagg agccctggcc
     2161 gtggaattga tggtatctgt tttgcagcat ccagaagggg gctatgccat tgccagcagc
     2221 agtgacgatc ggatgaatga gcctccaacc tctcttgggc ttgtgcctca ccagatccgg
     2281 ggatttcttt cacggtttga taatgtcctt cccgtcagcc tggcatttga caaatgtaca
     2341 gcttgttctt ccaaagttct tgatcaatat gaacgagaag gatttaactt cctagccaag
     2401 gtgtttaatt cttcacattc cttcttagaa gacttgactg gtcttacatt gctgcatcaa
     2461 gaaacccaag ctgctgagat ctgggacatg agcgatgatg agaccatctg agatggcccc
     2521 gctgtggggc tgacttctcc ccggccgcct gctgaggagc tctccatcgc cagagcagga
     2581 ctgctgaccc caggcctggt gattctgggc ccctcctcca taccccgagg tctgggattc
     2641 ccccctctgc tgcccaggag tggccagtgt tcggcgttgc tcgggattca agataccacc
     2701 agttcagagc taaataataa ccttggcctt ggccttgcta ttgacctggg acttggtcct
     2761 ccatgcagtt tttatttctt gtcacagtga ctgatagcca tcccccagga tcctttcccc
     2821 ttggccctga gggggtgacc caacacagac caaatgggga aatgagcaac cagctcctgc
     2881 ccagagccac tgcgggaggt ggcaccctca tccccggaat gtgctgccca ccgcaccgca
     2941 ggctcctcct gtgggggccc tgggcatggg tgagggtggg accccgtgag cgcactgcac
     3001 cctggccctg gtggagcggg aggaggagga gagccgagct gggtacgaga ctaaagggcc
     3061 cacatgaccc agtgacgcca gatttccacc aaggactgag tgagctgctc agacatggct
     3121 ttctgcctcc cagcctgtcc tccactgtgg gcatagcatc tgtgcctgcc tgcctgcttg
     3181 agggagagga gtttctgctg ctgccttgag ctggggggaa gagcccaggg gcagatcctg
     3241 gcagctgcct ggatggggct cctccctgcc cttatgagca ggccaggccc agaaaggccg
     3301 agcctgggct gccttcctgc cccagccgag ggaggggtca gacggctcta ccatgggtaa
     3361 ctcaggcaag agctggtttt cctctttatt ctgggtgtgt gcagctgtga ggccccaacc
     3421 caggagaggc catggcctag gtacctgtga ccaccctgcc cccgtgtaga gggcatcgtc
     3481 tttcctgcta ttttattctt tcagcttttg tcttaggccc agaatcaaag tgaaaattga
     3541 gtcgagctga cccttacaac agtaggattt agtagggtag atttcaaatg aggcttcgct
     3601 tctcccaaag tagccagtcc aagttccagt ggctgtcgtt cagctcatgg gagcttcatg
     3661 gggacacagc cggcacaggt gcagggcccg agtccgccca cccagcctgg cgctgaaact
     3721 gcacacgtac actatgtggt ttaagagcac tttattattg ttcttaaggc tacttttaag
     3781 tacaaaaaaa gatggcctgc caaacctttt tttttcttct tccaggaaaa acaggccaca
     3841 gagaatggta tattacagat ttacacacat gaagagaagg tcagagcgca ctgcaggcag
     3901 cgcggctctg ggaagaactt cacggagccc cttcttagag cagggagggg gctttctcag
     3961 tgaaatgttt ggttttctgc tgcctcctct gccccaggcc cccctccagg gtactgccta
     4021 tcccagatag gtcagtgcac cagggacccg gccgccagca ccgccgaccc ctcccagagt
     4081 gacgcccttg ttcactgaca aagagacctg tcccaggagt gtcctccacc gagccggtca
     4141 gctgtgggtg gttttcctgt tacgacgctc agtagcctgt agcaataaca aactcgtggc
     4201 tatgaatgca gatgcagtgt tctcatagaa taactgttcc tgcactttta cagacaaatc
     4261 tacgacaaaa aaaaagatca actttttttt tccgaacaac aaaaaaaatg aatgattaca
     4321 ataggaaagg gaaaaattaa atagctacat atcattaaca aattaatgtt cttcaaaaaa
     4381 tacctacaaa tttctctgta cattctttac gcacagcgta acgatggtct caaaatcacc
     4441 catatagaaa agtgttctca acgatttttc ctacagaaaa tataggggcc tgaatgccaa
     4501 agcttggaag cccagtacag tgggagtgaa atgtgtgcgg ggcaaggaga agggcttttc
     4561 tttcctccac ttttcaaagg cctgcagcca ctctgtgact acaagagcca gtcctccgac
     4621 cttttcaccc agtgccaatt tccaaaattc aacagctaaa aactgtaaaa ccgggggtca
     4681 tacggtgtgc agagtccaca aagccttgca ggtgaggtga ccacgcccac gtcacctggt
     4741 caggtgccat cgtcgtgagc ctctggtggg ccaggtggga cacagcacac cccaggggga
     4801 ggggatagaa acgctcattg accaaaaagg agcagctgtg acctccacag ctgtgtctgt
     4861 catgcttgct tcatctaatt tctagttagt agctattaat atagcaaata ataaatgcag
     4921 taataacagt ataaagtcag aggaatgtat actgccttgg ccccagcgta cgaggaagcg
     4981 tataaaacac catatcacag attgtctgtc agtaatctgc tgttcagcca agagagttca
     5041 aagggagcag tttctgcatg tagggaagtt ggaagacaca aaccccacct cccctgggag
     5101 cttgtaacaa agcagacagg gatgcaaaaa taaatgatgt cagcctgcag ccaaactcca
     5161 gcatcccaca ccgcagctga cccactgctc atcgcgaggg cctgccagga gctggcctcc
     5221 cgcactactt gtgagtaaag tgaatatcaa ataccaatct tagagtacaa ctgtaccagc
     5281 agtaagtata tctaggactg taactgacaa aaataaacta attctgaaaa gaa
//
