LOCUS       NM_022488               1568 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens autophagy related 3 (ATG3), transcript variant 1,
            mRNA.
ACCESSION   NM_022488
VERSION     NM_022488.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Farnung,J., Muhar,M., Liang,J.R., Tolmachova,K.A., Benoit,R.M.,
            Corn,J.E. and Bode,J.W.
  TITLE     Semisynthetic LC3 Probes for Autophagy Pathways Reveal a
            Noncanonical LC3 Interacting Region Motif Crucial for the Enzymatic
            Activity of Human ATG3
  JOURNAL   ACS Cent Sci 9 (5), 1025-1034 (2023)
   PUBMED   37252361
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1568)
  AUTHORS   da Silva Lima,N., Fondevila,M.F., Novoa,E., Buque,X.,
            Mercado-Gomez,M., Gallet,S., Gonzalez-Rellan,M.J., Fernandez,U.,
            Loyens,A., Garcia-Vence,M., Chantada-Vazquez,M.D.P., Bravo,S.B.,
            Maranon,P., Senra,A., Escudero,A., Leiva,M., Guallar,D.,
            Fidalgo,M., Gomes,P., Claret,M., Sabio,G., Varela-Rey,M.,
            Delgado,T.C., Montero-Vallejo,R., Ampuero,J., Lopez,M., Dieguez,C.,
            Herrero,L., Serra,D., Schwaninger,M., Prevot,V., Gallego-Duran,R.,
            Romero-Gomez,M., Iruzubieta,P., Crespo,J., Martinez-Chantar,M.L.,
            Garcia-Monzon,C., Gonzalez-Rodriguez,A., Aspichueta,P. and
            Nogueiras,R.
  TITLE     Inhibition of ATG3 ameliorates liver steatosis by increasing
            mitochondrial function
  JOURNAL   J Hepatol 76 (1), 11-24 (2022)
   PUBMED   34555423
  REMARK    GeneRIF: Inhibition of ATG3 ameliorates liver steatosis by
            increasing mitochondrial function.
REFERENCE   4  (bases 1 to 1568)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 1568)
  AUTHORS   Ye,Y., Tyndall,E.R., Bui,V., Tang,Z., Shen,Y., Jiang,X.,
            Flanagan,J.M., Wang,H.G. and Tian,F.
  TITLE     An N-terminal conserved region in human Atg3 couples membrane
            curvature sensitivity to conjugase activity during autophagy
  JOURNAL   Nat Commun 12 (1), 374 (2021)
   PUBMED   33446636
  REMARK    GeneRIF: An N-terminal conserved region in human Atg3 couples
            membrane curvature sensitivity to conjugase activity during
            autophagy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1568)
  AUTHORS   Tanida,I., Ueno,T. and Kominami,E.
  TITLE     Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal
            Met121 to expose Gly120 for lipidation and targeting to
            autophagosomal membranes
  JOURNAL   J Biol Chem 279 (46), 47704-47710 (2004)
   PUBMED   15355958
REFERENCE   7  (bases 1 to 1568)
  AUTHORS   Brill,L.M., Salomon,A.R., Ficarro,S.B., Mukherji,M.,
            Stettler-Gill,M. and Peters,E.C.
  TITLE     Robust phosphoproteomic profiling of tyrosine phosphorylation sites
            from human T cells using immobilized metal affinity chromatography
            and tandem mass spectrometry
  JOURNAL   Anal Chem 76 (10), 2763-2772 (2004)
   PUBMED   15144186
REFERENCE   8  (bases 1 to 1568)
  AUTHORS   Nemoto,T., Tanida,I., Tanida-Miyake,E., Minematsu-Ikeguchi,N.,
            Yokota,M., Ohsumi,M., Ueno,T. and Kominami,E.
  TITLE     The mouse APG10 homologue, an E2-like enzyme for Apg12p
            conjugation, facilitates MAP-LC3 modification
  JOURNAL   J Biol Chem 278 (41), 39517-39526 (2003)
   PUBMED   12890687
REFERENCE   9  (bases 1 to 1568)
  AUTHORS   Tanida,I., Nishitani,T., Nemoto,T., Ueno,T. and Kominami,E.
  TITLE     Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates
            LC3 processing
  JOURNAL   Biochem Biophys Res Commun 296 (5), 1164-1170 (2002)
   PUBMED   12207896
REFERENCE   10 (bases 1 to 1568)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Komatsu,M., Ueno,T. and Kominami,E.
  TITLE     Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple
            substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the
            conjugation of hApg12p to hApg5p
  JOURNAL   J Biol Chem 277 (16), 13739-13744 (2002)
   PUBMED   11825910
  REMARK    GeneRIF: Human Apg3p/Aut1p homologue is an authentic E2 enzyme for
            multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates
            the conjugation of hApg12p to hApg5p
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC311625.1, AC092692.6,
            AW408464.1 and BC024221.2.
            
            On May 17, 2019 this sequence version replaced NM_022488.4.
            
            Summary: This gene encodes a ubiquitin-like-conjugating enzyme and
            is a component of ubiquitination-like systems involved in
            autophagy, the process of degradation, turnover and recycling of
            cytoplasmic constituents in eukaryotic cells. This protein is known
            to play a role in regulation of autophagy during cell death. A
            pseudogene of this gene is located on chromosome 20. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163658.192031.1, AL137515.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000283290.10/ ENSP00000283290.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               DC311625.1         2-104
            104-104             AC092692.6         57545-57545         c
            105-324             AW408464.1         13-232
            325-1566            BC024221.2         1-1242
            1567-1568           AC092692.6         28196-28197         c
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.2"
     gene            1..1568
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /note="autophagy related 3"
                     /db_xref="GeneID:64422"
                     /db_xref="HGNC:HGNC:20962"
                     /db_xref="MIM:609606"
     exon            1..506
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     CDS             435..1379
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ubiquitin-like-conjugating enzyme ATG3; autophagy-related
                     protein 3; ATG3 autophagy related 3 homolog; APG3
                     autophagy 3-like"
                     /codon_start=1
                     /product="ubiquitin-like-conjugating enzyme ATG3 isoform
                     1"
                     /protein_id="NP_071933.2"
                     /db_xref="CCDS:CCDS2966.1"
                     /db_xref="GeneID:64422"
                     /db_xref="HGNC:HGNC:20962"
                     /db_xref="MIM:609606"
                     /translation="MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGD
                     HLVHHCPTWQWATGEELKVKAYLPTGKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDD
                     GDGGWVDTYHNTGITGITEAVKEITLENKDNIRLQDCSALCEEEEDEDEGEAADMEEY
                     EESGLLETDEATLDTRKIVEACKAKTDAGGEDAILQTRTYDLYITYDKYYQTPRLWLF
                     GYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIET
                     VAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM"
     exon            507..548
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            549..598
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            599..669
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            670..777
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            778..827
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            828..909
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            910..944
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1100
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1228
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1297
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1568
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1547..1552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /note="hexamer: AATAAA"
     polyA_site      1568
                     /gene="ATG3"
                     /gene_synonym="APG3; APG3-LIKE; APG3L; hApg3; PC3-96"
                     /note="major polyA site"
ORIGIN      
        1 agtgtcacgt gaggccccgg tggcggcgca gctacggcaa gagagtgaga aggaagggaa
       61 gccggaaggg gcgcgagtga agcaaagcga ggacagacag ctcgcagagg gcgaggggtg
      121 cgtgtgcgtc cgcttctcac ctcaggtctc ccttcggccc cgctgccctc cctcgcggct
      181 gggtgacagc tgggtccggt ccgtcgcggg ctgcctgggg tgcgaggatc gcgcaccccg
      241 tcttcgcgcg ctgtgcctgc cgccccgccc cctcgtcccg cccgtcccgt cgcgtcgcgt
      301 cccgtcccct cgggtgctgc cagccgggtg ctgatgcgag tcggtggcag cgaggacatt
      361 ttctgactcc ctggcccctg acacggctgc actttccatc ccgtcgcggg gccggccgct
      421 actccggccc caggatgcag aatgtgatta atactgtgaa gggaaaggca ctggaagtgg
      481 ctgagtacct gaccccggtc ctcaaggaat caaagtttaa ggaaacaggt gtaattaccc
      541 cagaagagtt tgtggcagct ggagatcacc tagtccacca ctgtccaaca tggcaatggg
      601 ctacagggga agaattgaaa gtgaaggcat acctaccaac aggcaaacaa tttttggtaa
      661 ccaaaaatgt gccgtgctat aagcggtgca aacagatgga atattcagat gaattggaag
      721 ctatcattga agaagatgat ggtgatggcg gatgggtaga tacatatcac aacacaggta
      781 ttacaggaat aacggaagcc gttaaagaga tcacactgga aaataaggac aatataaggc
      841 ttcaagattg ctcagcacta tgtgaagagg aagaagatga agatgaagga gaagctgcag
      901 atatggaaga atatgaagag agtggattgt tggaaacaga tgaggctacc ctagatacaa
      961 ggaaaatagt agaagcttgt aaagccaaaa ctgatgctgg cggtgaagat gctattttgc
     1021 aaaccagaac ttatgacctt tacatcactt atgataaata ttaccagact ccacgattat
     1081 ggttgtttgg ctatgatgag caacggcagc ctttaacagt tgagcacatg tatgaagaca
     1141 tcagtcagga tcatgtgaag aaaacagtga ccattgaaaa tcaccctcat ctgccaccac
     1201 ctcccatgtg ttcagttcac ccatgcaggc atgctgaggt gatgaagaaa atcattgaga
     1261 ctgttgcaga aggaggggga gaacttggag ttcatatgta tcttcttatt ttcttgaaat
     1321 ttgtacaagc tgtcattcca acaatagaat atgactacac aagacacttc acaatgtaat
     1381 gaagagagca taaaatctat cctaattatt ggttctgatt tttaaagaat taacccatag
     1441 atgtgaccat tgaccatatt catcaatata tacagtttct ctaataaggg acttatatgt
     1501 ttatgcatta aataaaaata tgttccacta ccagccttac ttgtttaata aaaatcagtg
     1561 caaagaga
//
