LOCUS       NM_004707               4040 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens autophagy related 12 (ATG12), transcript variant 1,
            mRNA.
ACCESSION   NM_004707
VERSION     NM_004707.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Zhang,R., Hao,J., Yu,H., Wang,Z.J., Lan,F., Peng,Y. and Qiu,Y.
  TITLE     circ_SIRT1 upregulates ATG12 to facilitate Imatinib resistance in
            CML through interacting with EIF4A3
  JOURNAL   Gene 893, 147917 (2024)
   PUBMED   37866664
  REMARK    GeneRIF: circ_SIRT1 upregulates ATG12 to facilitate Imatinib
            resistance in CML through interacting with EIF4A3.
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Keulers,T.G., Koch,A., van Gisbergen,M.W., Barbeau,L.M.O.,
            Zonneveld,M.I., de Jong,M.C., Savelkouls,K.G.M., Wanders,R.G.,
            Bussink,J., Melotte,V. and Rouschop,K.M.A.
  TITLE     ATG12 deficiency results in intracellular glutamine depletion,
            abrogation of tumor hypoxia and a favorable prognosis in cancer
  JOURNAL   Autophagy 18 (8), 1898-1914 (2022)
   PUBMED   34904929
  REMARK    GeneRIF: ATG12 deficiency results in intracellular glutamine
            depletion, abrogation of tumor hypoxia and a favorable prognosis in
            cancer.
REFERENCE   3  (bases 1 to 4040)
  AUTHORS   Zhang,S., Liu,X., Abdulmomen Ali Mohammed,S., Li,H., Cai,W.,
            Guan,W., Liu,D., Wei,Y., Rong,D., Fang,Y., Haider,F., Lv,H.,
            Jin,Z., Chen,X., Mo,Z., Li,L., Yang,S. and Wang,H.
  TITLE     Adaptor SH3BGRL drives autophagy-mediated chemoresistance through
            promoting PIK3C3 translation and ATG12 stability in breast cancers
  JOURNAL   Autophagy 18 (8), 1822-1840 (2022)
   PUBMED   34870550
  REMARK    GeneRIF: Adaptor SH3BGRL drives autophagy-mediated chemoresistance
            through promoting PIK3C3 translation and ATG12 stability in breast
            cancers.
REFERENCE   4  (bases 1 to 4040)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 4040)
  AUTHORS   Dai,H., Wang,J., Huang,Z., Zhang,H., Wang,X., Li,Q. and Feng,W.
  TITLE     LncRNA OIP5-AS1 Promotes the Autophagy-Related Imatinib Resistance
            in Chronic Myeloid Leukemia Cells by Regulating miR-30e-5p/ATG12
            Axis
  JOURNAL   Technol Cancer Res Treat 20, 15330338211052150 (2021)
   PUBMED   34723728
  REMARK    GeneRIF: LncRNA OIP5-AS1 Promotes the Autophagy-Related Imatinib
            Resistance in Chronic Myeloid Leukemia Cells by Regulating
            miR-30e-5p/ATG12 Axis.
REFERENCE   6  (bases 1 to 4040)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Komatsu,M., Ueno,T. and Kominami,E.
  TITLE     Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple
            substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the
            conjugation of hApg12p to hApg5p
  JOURNAL   J Biol Chem 277 (16), 13739-13744 (2002)
   PUBMED   11825910
  REMARK    GeneRIF: Human Apg3p/Aut1p homologue is an authentic E2 enzyme for
            multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates
            the conjugation of hApg12p to hApg5p
REFERENCE   7  (bases 1 to 4040)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Nishitani,T., Komatsu,M., Yamazaki,H.,
            Ueno,T. and Kominami,E.
  TITLE     Murine Apg12p has a substrate preference for murine Apg7p over
            three Apg8p homologs
  JOURNAL   Biochem Biophys Res Commun 292 (1), 256-262 (2002)
   PUBMED   11890701
REFERENCE   8  (bases 1 to 4040)
  AUTHORS   Tanida,I., Tanida-Miyake,E., Ueno,T. and Kominami,E.
  TITLE     The human homolog of Saccharomyces cerevisiae Apg7p is a
            Protein-activating enzyme for multiple substrates including human
            Apg12p, GATE-16, GABARAP, and MAP-LC3
  JOURNAL   J Biol Chem 276 (3), 1701-1706 (2001)
   PUBMED   11096062
REFERENCE   9  (bases 1 to 4040)
  AUTHORS   Mizushima,N., Sugita,H., Yoshimori,T. and Ohsumi,Y.
  TITLE     A new protein conjugation system in human. The counterpart of the
            yeast Apg12p conjugation system essential for autophagy
  JOURNAL   J Biol Chem 273 (51), 33889-33892 (1998)
   PUBMED   9852036
REFERENCE   10 (bases 1 to 4040)
  AUTHORS   Ueno,K., Kumagai,T., Kijima,T., Kishimoto,T. and Hosoe,S.
  TITLE     Cloning and tissue expression of cDNAs from chromosome 5q21-22
            which is frequently deleted in advanced lung cancer
  JOURNAL   Hum Genet 102 (1), 63-68 (1998)
   PUBMED   9490301
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC012266.2, AC026449.6 and DB530852.1.
            
            On Nov 24, 2018 this sequence version replaced NM_004707.3.
            
            Summary: Autophagy is a process of bulk protein degradation in
            which cytoplasmic components, including organelles, are enclosed in
            double-membrane structures called autophagosomes and delivered to
            lysosomes or vacuoles for degradation. ATG12 is the human homolog
            of a yeast protein involved in autophagy (Mizushima et al., 1998
            [PubMed 9852036]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longer protein
            (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803616.92489.1,
                                           SRR1803613.265879.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2149398 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000509910.2/ ENSP00000425107.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-661               BC012266.2         238-898
            662-3960            AC026449.6         51443-54741         c
            3961-4040           DB530852.1         229-308
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q22.3"
     gene            1..4040
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="autophagy related 12"
                     /db_xref="GeneID:9140"
                     /db_xref="HGNC:HGNC:588"
                     /db_xref="MIM:609608"
     exon            1..176
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..436
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="isoform 1 is encoded by transcript variant 1; Apg12
                     (autophagy, yeast) homolog; ubiquitin-like protein ATG12;
                     ATG12 autophagy related 12 homolog; APG12 autophagy
                     12-like"
                     /codon_start=1
                     /product="ubiquitin-like protein ATG12 isoform 1"
                     /protein_id="NP_004698.3"
                     /db_xref="CCDS:CCDS4122.2"
                     /db_xref="GeneID:9140"
                     /db_xref="HGNC:HGNC:588"
                     /db_xref="MIM:609608"
                     /translation="MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGT
                     EEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFI
                     YVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG"
     exon            177..313
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            314..376
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /inference="alignment:Splign:2.1.0"
     exon            377..4040
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      732..737
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="hexamer: TATAAA"
     polyA_site      761
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="major polyA site"
     regulatory      2986..2991
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="hexamer: AATAAA"
     polyA_site      3007
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="major polyA site"
     regulatory      4019..4024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
                     /note="hexamer: AATAAA"
     polyA_site      4040
                     /gene="ATG12"
                     /gene_synonym="APG12; APG12L; FBR93; HAPG12"
ORIGIN      
        1 gtctccaagc aagatggcgg aggagccgca gtctgtgttg cagcttccta cttcaattgc
       61 tgctggaggg gaaggactta cggatgtctc cccagaaaca accaccccgg agcccccgtc
      121 ttccgctgca gtttccccgg gaacagagga acctgctggc gacaccaaga aaaaaattga
      181 cattttgcta aaggctgtgg gagacactcc tattatgaaa acaaagaagt gggcagtaga
      241 gcgaacacga accatccaag gactcattga cttcatcaaa aagtttctta aacttgtggc
      301 ctcagaacag ttgtttattt atgtgaatca gtcctttgct ccttccccag accaagaagt
      361 tggaactctc tatgagtgtt ttggcagtga tggtaaactg gttttacatt actgcaagtc
      421 tcaggcgtgg ggatgaacca caaagaaaat caacttgcta ctacatgaaa tggattttca
      481 cggaagagac agctctgaaa agttttgatg cttgtggcaa gagacttaac agatgtgatc
      541 tatttagtat gtgtctactc tatgtttatg cataagaaaa catccatagc atgaatggac
      601 tcagaaaaat gtgatttgta ttaatgcacc agtcatcata aaagatggtc atgatagtac
      661 acccattgct cctacttgtt actattattg ctgcagatct gcctccaagg ttgaaaagga
      721 gactaagact gtataaacat cttcattgtc agttctcaaa atgactgaaa ttgttttcat
      781 ggtaaaagtt aatatactaa agggttcctt tttttttaat gtttacattt atctctatgt
      841 ttaccttttt agtcacattg acctgctggc tgaatacctc aaatagtcca gtagagggca
      901 gtccaccagg cagaaaaggt taggcgtttt ggtttcacat ctttgctggg gaataatagg
      961 ggaaatggct gtttttgcta atttttagct aatatctagc caggagagca agcacatagg
     1021 acagactgaa agactgtaat tttacacaat acacatggct taattatttt attgggatac
     1081 agaaaaatat aaattctgga caaataagtc atatacctgt tttcagtcct aacatttaag
     1141 gattcttgag tcccaatcac ataactgtgg tgttactctg tcatttatat ggtgtcaaaa
     1201 gcacttgatg agtaaaccca gtagcatctt tttgagtgtt tcataatgca ttttccaact
     1261 tgaaaacaat aattgaaaaa tagccttatt gtatatttta tgccatgact aaaagtgcca
     1321 tttttactga tgctattaga ctgataattt cttgaagtga aatttaacct ttttttctct
     1381 ttagtattat gtttataatg ccatattttt agaaagcatt ccagatcagg catggtggct
     1441 tacacctgta atcccagcac tttggaaggc tgaggtgtgg ggattgcgtg aagccacaag
     1501 tttgagacca gcctggttag caaggcaaga tccccaactc tacaaaaaaa taaaaattaa
     1561 aaaaaaatta ttaggctgca gaggcaagag gatcccctga gcccagaagt tcaagggtat
     1621 agtgagtcgt gattgtacca ctgcattcct gctgagcaac agagtgagac cccatctcaa
     1681 aaaagaaaaa aaaaggcatt ctagtaaatc gaatgtaatg tgaatggaat ttcaaaacag
     1741 gatctaagat ggtatgtagt agaattcaaa gtaatatcat tttaaagtta aatgagtatg
     1801 gaaaaggtct gttctctagt tttgtccagt tcagtttact gaaggaatat atttaattat
     1861 attcatatat ttaacaaata aaaatatgtt gaattttcgt attgtttgcc actgagggtt
     1921 cagatgatag acctcaaaaa atcgaaaata ctggttgaaa tttgtagcat ccatttagtt
     1981 attctttttg acctaaataa cttaatagtt tattaaatct aaggttagct aaatatgtag
     2041 ctaaccttat ttgttttctt tcctaacaac tctgaagaat acataggact ttgcactttt
     2101 tttttttttt ttttttttta aagagacagc gtttcggtct tgttgcccag gctggactgt
     2161 aatggcacaa tcttggctca ttgcaacctc tacctcccgg gttcaagtga ttctcctgcc
     2221 tcagcctcct gagtagctgg gattacaggc acctgccacc acacccgcct aattttttgt
     2281 tatttttagt ggagacaggg ttttaccatt ttggccaggc tggtctcaaa ctcctgactg
     2341 acctcaggtg atccacccgc cttggcctcc caaagtgctg ggattatagg tgtgagccac
     2401 cgtgccgggc ctggactttg tactttttca aatcatattt aattatttct tgatctttac
     2461 tcaaagaatt attctgcgct aaatttgcaa cattaaacaa taattaagcc tggatccgat
     2521 aagttattgg gtgactagaa cctacaacac aatttatttt taatttaaac tctcaagtct
     2581 gattaaactt gggggaaaaa ctgaacattc aggtgatatt ttaaattttc tttgtgacta
     2641 aatagatgtc attaatatca tgcttcatct tactgtgcat agatgaccca tggcacacaa
     2701 agtatatgta taatattgca atattgtact tccatcttta aaagtatgcc aaagacttca
     2761 taatgaattc accatattta aggaataagg caaattaatg tttatccgaa atcaaaatga
     2821 gattggataa ttcctggaat ctgatttctg taagtttcag tcctaaaggg accttaaata
     2881 tctactagaa cgaagtacag tgtatttctc agattacatg acattagtca accaatttag
     2941 ttgtaacaca aagtgagaaa gccttaggtg ttgaaggagt gtgaaaataa aaatctcttt
     3001 tcatgcagtt tctgcttcat tttccaagga ttataatatg ctgagtaaac ttttggcact
     3061 aaggaagcca gctacaggcc acgtaatgaa aactattcag aaaacagttc agcaaatact
     3121 actatttgaa tacagttcaa atcgtattta tataaatact ctgcctacat tatttaaccc
     3181 aaactggatt attcaccatt ctttgaagat gccttgtgtt ttctgttatc tacttctgct
     3241 cgtgcagttt acttacacct tcaccctttc aaatcctaac tcttcttcaa ggcctgattc
     3301 agattttaac tttttaaagg ctatctgaat cattcaagga gaagataccc tttctctcat
     3361 aaaaacactt agagcaaact accactatta aatcacttat tgcatactgc ctcagagcca
     3421 tgtgtccagt tattctggtt tttatctggt acccacacac acataatact tagtacattt
     3481 ccttatatgt aaataattag taatgtaaca catcgagtga atgaaattgt gattagcaaa
     3541 gtatgacagc taaaaacaaa ggaaattttg tatagtttaa gtttttgtac agcagaaggt
     3601 aatggaaaat atttgcaaca tatgacagtg tttttaatgt cttaagtata tgaagaattt
     3661 gtttcaattg gtaagaaaag accaactgaa aaataggcaa aacataccaa atttcgattc
     3721 acaaaaagat acaaatggcc aacaaaacat gaaacacctt gaaccccatt ggttacaaaa
     3781 aatacaaata aaaacaatgc tgactgacaa taaaaataat gtacatcttg ctattagcag
     3841 agttgtgggg aaatcctgtc atattttggt gaatagaata tgagttggta tatcactttt
     3901 agggggccat ttggcaatat caaaaacctt gaaacgtaag tataaacttc agtttgttcg
     3961 tataacctgt cattcacata ttgagtgcct actatgtcct aaatattgga ataacactaa
     4021 taaagctcat aagagactaa
//
