LOCUS       NM_030632              11760 bp    mRNA    linear   PRI 09-FEB-2025
DEFINITION  Homo sapiens ASXL transcriptional regulator 3 (ASXL3), mRNA.
ACCESSION   NM_030632 XM_290811 XM_937373
VERSION     NM_030632.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Schirwani,S., Woods,E., Koolen,D.A., Ockeloen,C.W., Lynch,S.A.,
            Kavanagh,K., Graham,J.M. Jr., Grand,K., Pierson,T.M., Chung,J.M.
            and Balasubramanian,M.
  TITLE     Familial Bainbridge-Ropers syndrome: Report of familial ASXL3
            inheritance and a milder phenotype
  JOURNAL   Am J Med Genet A 191 (1), 29-36 (2023)
   PUBMED   36177608
  REMARK    GeneRIF: Familial Bainbridge-Ropers syndrome: Report of familial
            ASXL3 inheritance and a milder phenotype.
REFERENCE   2  (bases 1 to 11760)
  AUTHORS   Schirwani,S., Albaba,S., Carere,D.A., Guillen Sacoto,M.J., Milan
            Zamora,F., Si,Y., Rabin,R., Pappas,J., Renaud,D.L., Hauser,N.,
            Reid,E., Blanchet,P., Foulds,N., Dixit,A., Fisher,R., Armstrong,R.,
            Isidor,B., Cogne,B., Schrier Vergano,S., Demirdas,S., Dykzeul,N.,
            Cohen,J.S., Grand,K., Morel,D., Slavotinek,A., Albassam,H.F.,
            Naik,S., Dean,J., Ragge,N., Costa,C., Tedesco,M.G., Harrison,R.E.,
            Bouman,A., Palen,E., Challman,T.D., Willemsen,M.H., Vogt,J.,
            Cunniff,C., Bergstrom,K., Walia,J.S., Bruel,A.L., Kini,U.,
            Alkuraya,F.S., Slegesky,V., Meeks,N., Girotto,P., Johnson,D.,
            Newbury-Ecob,R., Ockeloen,C.W., Prontera,P., Lynch,S.A., Li,D.,
            Graham,J.M. Jr., Pierson,T.M. and Balasubramanian,M.
  CONSRTM   DDD Study
  TITLE     Expanding the phenotype of ASXL3-related syndrome: A comprehensive
            description of 45 unpublished individuals with inherited and de
            novo pathogenic variants in ASXL3
  JOURNAL   Am J Med Genet A 185 (11), 3446-3458 (2021)
   PUBMED   34436830
  REMARK    GeneRIF: Expanding the phenotype of ASXL3-related syndrome: A
            comprehensive description of 45 unpublished individuals with
            inherited and de novo pathogenic variants in ASXL3.
            Erratum:[Am J Med Genet A. 2023 Jan;191(1):310. doi:
            10.1002/ajmg.a.62993.. PMID: 36222436]
REFERENCE   3  (bases 1 to 11760)
  AUTHORS   Cuddapah,V.A., Dubbs,H.A., Adang,L., Kugler,S.L., McCormick,E.M.,
            Zolkipli-Cunningham,Z., Ortiz-Gonzalez,X.R., McCormack,S.,
            Zackai,E., Licht,D.J., Falk,M.J. and Marsh,E.D.
  TITLE     Understanding the phenotypic spectrum of ASXL-related disease: Ten
            cases and a review of the literature
  JOURNAL   Am J Med Genet A 185 (6), 1700-1711 (2021)
   PUBMED   33751773
  REMARK    GeneRIF: Understanding the phenotypic spectrum of ASXL-related
            disease: Ten cases and a review of the literature.
REFERENCE   4  (bases 1 to 11760)
  AUTHORS   Yu,K.P., Luk,H.M., Fung,J.L.F., Chung,B.H. and Lo,I.F.
  TITLE     Further expanding the clinical phenotype in Bainbridge-Ropers
            syndrome and dissecting genotype-phenotype correlation in the ASXL3
            mutational cluster regions
  JOURNAL   Eur J Med Genet 64 (1), 104107 (2021)
   PUBMED   33242595
  REMARK    GeneRIF: Further expanding the clinical phenotype in
            Bainbridge-Ropers syndrome and dissecting genotype-phenotype
            correlation in the ASXL3 mutational cluster regions.
REFERENCE   5  (bases 1 to 11760)
  AUTHORS   Dinwiddie,D.L., Soden,S.E., Saunders,C.J., Miller,N.A.,
            Farrow,E.G., Smith,L.D. and Kingsmore,S.F.
  TITLE     De novo frameshift mutation in ASXL3 in a patient with global
            developmental delay, microcephaly, and craniofacial anomalies
  JOURNAL   BMC Med Genomics 6, 32 (2013)
   PUBMED   24044690
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11760)
  AUTHORS   Katoh,M.
  TITLE     Functional and cancer genomics of ASXL family members
  JOURNAL   Br J Cancer 109 (2), 299-306 (2013)
   PUBMED   23736028
  REMARK    Review article
REFERENCE   7  (bases 1 to 11760)
  AUTHORS   Bainbridge,M.N., Hu,H., Muzny,D.M., Musante,L., Lupski,J.R.,
            Graham,B.H., Chen,W., Gripp,K.W., Jenny,K., Wienker,T.F., Yang,Y.,
            Sutton,V.R., Gibbs,R.A. and Ropers,H.H.
  TITLE     De novo truncating mutations in ASXL3 are associated with a novel
            clinical phenotype with similarities to Bohring-Opitz syndrome
  JOURNAL   Genome Med 5 (2), 11 (2013)
   PUBMED   23383720
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 11760)
  AUTHORS   Fisher,C.L., Randazzo,F., Humphries,R.K. and Brock,H.W.
  TITLE     Characterization of Asxl1, a murine homolog of Additional sex
            combs, and analysis of the Asx-like gene family
  JOURNAL   Gene 369, 109-118 (2006)
   PUBMED   16412590
REFERENCE   9  (bases 1 to 11760)
  AUTHORS   Katoh,M. and Katoh,M.
  TITLE     Identification and characterization of ASXL3 gene in silico
  JOURNAL   Int J Oncol 24 (6), 1617-1622 (2004)
   PUBMED   15138607
REFERENCE   10 (bases 1 to 11760)
  AUTHORS   Balasubramanian,M. and Schirwani,S.
  TITLE     ASXL3-Related Disorder
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   33151654
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC023192.8, BE145544.1,
            AK131454.1, DB090653.1 and AC010798.9.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_030632.2.
            
            Summary: This gene encodes a protein containing a plant homeodomain
            (PHD) zinc finger domain that plays a role in the regulation of
            gene transcription. The encoded protein has been shown to
            negatively regulate lipogenesis by binding to and inhibiting the
            transcriptional activity of two nuclear hormone receptors,
            oxysterols receptor LXR-alpha (LXRalpha) and thyroid hormone
            receptor beta (TRbeta). The encoded protein may also inhibit
            histone deubiquitination. Mutations in this gene have been
            identified in human patients with Bainbridge-Ropers syndrome, which
            is characterized by feeding difficulties, developmental delay and
            other features. [provided by RefSeq, May 2017].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA2151119,
                              SAMN03267750 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000269197.12/ ENSP00000269197.4
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-358               AC023192.8         59522-59879         c
            359-396             BE145544.1         1-38
            397-550             AK131454.1         1-154
            551-914             DB090653.1         167-530
            915-1292            AK131454.1         522-899
            1293-1510           AK131454.1         978-1195
            1511-1511           AC010798.9         61514-61514
            1512-2591           AK131454.1         1197-2276
            2592-2592           AC010798.9         62595-62595
            2593-4126           AK131454.1         2278-3811
            4127-11760          AC010798.9         66574-74207
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q12.1"
     gene            1..11760
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /note="ASXL transcriptional regulator 3"
                     /db_xref="GeneID:80816"
                     /db_xref="HGNC:HGNC:29357"
                     /db_xref="MIM:615115"
     exon            1..467
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     CDS             414..7160
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /note="additional sex combs-like protein 3; additional sex
                     combs like transcriptional regulator 3; additional sex
                     combs like 3, transcriptional regulator"
                     /codon_start=1
                     /product="putative Polycomb group protein ASXL3"
                     /protein_id="NP_085135.1"
                     /db_xref="CCDS:CCDS45847.1"
                     /db_xref="GeneID:80816"
                     /db_xref="HGNC:HGNC:29357"
                     /db_xref="MIM:615115"
                     /translation="MKDKRKKKDRTWAEAARLALEKHPNSPMTAKQILEVIQKEGLKE
                     TSGTSPLACLNAMLHTNTRIGDGTFFKIPGKSGLYALKKEESSCPADGTLDLVCESEL
                     DGTDMAEANAHGEENGVCSKQVTDEASSTRDSSLTNTAVQSKLVSSFQQHTKKALKQA
                     LRQQQKRRNGVSMMVNKTVPRVVLTPLKVSDEQSDSPSGSESKNGEADSSDKEMKHGQ
                     KSPTGKQTSQHLKRLKKSGLGHLKWTKAEDIDIETPGSILVNTNLRALINKHTFASLP
                     QHFQQYLLLLLPEVDRQMGSDGILRLSTSALNNEFFAYAAQGWKQRLAEGEFTPEMQL
                     RIRQEIEKEKKTEPWKEKFFERFYGEKLGMSREESVKLTTGPNNAGAQSSSSCGTSGL
                     PVSAQTALAEQQPKSMKSPASPEPGFCATLCPMVEIPPKDIMAELESEDILIPEESVI
                     QEEIAEEVETSICECQDENHKTIPEFSEEAESLTNSHEEPQIAPPEDNLESCVMMNDV
                     LETLPHIEVKIEGKSESPQEEMTVVIDQLEVCDSLIPSTSSMTHVSDTEHKESETAVE
                     TSTPKIKTGSSSLEGQFPNEGIAIDMELQSDPEEQLSENACISETSFSSESPEGACTS
                     LPSPGGETQSTSEESCTPASLETTFCSEVSSTENTDKYNQRNSTDENFHASLMSEISP
                     ISTSPEISEASLMSNLPLTSEASPVSNLPLTSETSPMSDLPLTSETSSVSSMLLTSET
                     TFVSSLPLPSETSPISNSSINERMAHQQRKSPSVSEEPLSPQKDESSATAKPLGENLT
                     SQQKNLSNTPEPIIMSSSSIAPEAFPSEDLHNKTLSQQTCKSHVDTEKPYPASIPELA
                     STEMIKVKNHSVLQRTEKKVLPSPLELSVFSEGTDNKGNELPSAKLQDKQYISSVDKA
                     PFSEGSRNKTHKQGSTQSRLETSHTSKSSEPSKSPDGIRNESRDSEISKRKTAEQHSF
                     GICKEKRARIEDDQSTRNISSSSPPEKEQPPREEPRVPPLKIQLSKIGPPFIIKSQPV
                     SKPESRASTSTSVSGGRNTGARTLADIKARAQQARAQREAAAAAAVAAAASIVSGAMG
                     SPGEGGKTRTLAHIKEQTKAKLFAKHQARAHLFQTSKETRLPPPLSSKEGPPNLEVSS
                     TPETKMEGSTGVIIVNPNCRSPSNKSAHLRETTTVLQQSLNPSKLPETATDLSVHSSD
                     ENIPVSHLSEKIVSSTSSENSSVPMLFNKNSVPVSVCSTAISGAIKEHPFVSSVDKSS
                     VLMSVDSANTTISACNISMLKTIQGTDTPCIAIIPKCIESTPISATTEGSSISSSMDD
                     KQLLISSSSASNLVSTQYTSVPTPSIGNNLPNLSTSSVLIPPMGINNRFPSEKIAIPG
                     SEEQATVSMGTTVRAALSCSDSVAVTDSLVAHPTVAMFTGNMLTINSYDSPPKLSAES
                     LDKNSGPRNRADNSGKPQQPPGGFAPAAINRSIPCKVIVDHSTTLTSSLSLTVSVESS
                     EASLDLQGRPVRTEASVQPVACPQVSVISRPEPVANEGIDHSSTFIAASAAKQDSKTL
                     PATCTSLRELPLVPDKLNEPTAPSHNFAEQARGPAPFKSEADTTCSNQYNPSNRICWN
                     DDGMRSTGQPLVTHSGSSKQKEYLEQSCPKAIKTEHANYLNVSELHPRNLVTNVALPV
                     KSELHEADKGFRMDTEDFPGPELPPPAAEGASSVQQTQNMKASTSSPMEEAISLATDA
                     LKRVPGAGSSGCRLSSVEANNPLVTQLLQGNLPLEKVLPQPRLGAKLEINRLPLPLQT
                     TSVGKTAPERNVEIPPSSPNPDGKGYLAGTLAPLQMRKRENHPKKRVARTVGEHTQVK
                     CEPGKLLVEPDVKGVPCVISSGISQLGHSQPFKQEWLNKHSMQNRIVHSPEVKQQKRL
                     LPSCSFQQNLFHVDKNGGFHTDAGTSHRQQFYQMPVAARGPIPTAALLQASSKTPVGC
                     NAFAFNRHLEQKGLGEVSLSSAPHQLRLANMLSPNMPMKEGDEVGGTAHTMPNKALVH
                     PPPPPPPPPPPPLALPPPPPPPPPLPPPLPNAEVPSDQKQPPVTMETTKRLSWPQSTG
                     ICSNIKSEPLSFEEGLSSSCELGMKQVSYDQNEMKEQLKAFALKSADFSSYLLSEPQK
                     PFTQLAAQKMQVQQQQQLCGNYPTIHFGSTSFKRAASAIEKSIGILGSGSNPATGLSG
                     QNAQMPVQNFADSSNADELELKCSCRLKAMIVCKGCGAFCHDDCIGPSKLCVACLVVR
                     "
     exon            468..550
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            551..659
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            660..768
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            769..890
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1008
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1128
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1292
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1389
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1495
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..3452
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     exon            3453..11760
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11743..11748
                     /regulatory_class="polyA_signal_sequence"
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /note="hexamer: AAGAAA"
     polyA_site      11760
                     /gene="ASXL3"
                     /gene_synonym="BRPS; KIAA1713"
                     /note="major polyA site"
ORIGIN      
        1 agagtgtatt gttgtgagcg gggccgagcg gagcatcccc ggagctgtca ccgcagcggc
       61 cgcggcggtg gcgcagcgcg agccccgcac gagcgagccc ggccggcgcc gcccccgccc
      121 ctggcccggg ccccgctgct gccgccgccc ccgcccccgg cccgcccgcc gcgcgccgcc
      181 gccgccgccg tcgcgcgccc ccacccactc caccccaccc cctcgctcca tccctcccac
      241 ccgccgccgc cgccgccgcc gccgccgccg ccgccgccgc caccgcccgc gcgcctcccc
      301 ccgcggagcg agtgcgggtc accgggtcac tgggtcattg tctccgcgcc cgaaccccga
      361 gcaccccgtg gaatccccca cgtcatcatc agaaccatca atgagatgca aacatgaaag
      421 acaagaggaa gaagaaggac cgcacctggg ccgaggctgc ccgcctggca ctagaaaaac
      481 accccaactc accaatgaca gcaaagcaga tattggaagt cattcagaaa gaagggttaa
      541 aagaaacaag tggaacctct ccattagcct gtctgaatgc aatgcttcac actaacactc
      601 gaatagggga tggaacattc ttcaaaatcc ctggaaagtc aggcctctat gctctcaaaa
      661 aagaggagtc gtcatgccca gcagatggca cgttggattt agtctgtgaa tctgaattgg
      721 atggtacaga tatggccgag gcaaatgccc atggagaaga aaatggagtt tgttcgaagc
      781 aggtaactga tgaagcatct tccactcgag attcaagcct tactaacaca gcagtgcaaa
      841 gcaagttagt gtcttccttc cagcagcaca ccaaaaaggc tcttaaacag gctttgaggc
      901 agcagcagaa aagaagaaat ggagtctcaa tgatggtaaa caagactgtt cctcgtgttg
      961 ttttgacacc attaaaggtg tctgatgagc agtcggattc gccttcagga tctgaatcta
     1021 aaaatggtga agcagacagt tcagataaag aaatgaaaca tgggcaaaaa tctcccactg
     1081 gaaaacaaac aagtcagcac ttaaaacgat taaaaaagtc tggtttaggg cacttgaaat
     1141 ggaccaaagc tgaggacatt gacatagaaa ccccaggatc tattcttgtc aacactaact
     1201 tgagggcatt aataaataaa catacgtttg cttccttacc tcagcatttt caacaatacc
     1261 tcctgctttt gctcccagaa gtggataggc agatgggaag tgatggaatt ttacgcctca
     1321 gtacttcagc tctaaataat gaattctttg catatgcagc acaagggtgg aaacagcgac
     1381 tggcagaagg agagtttacc ccagaaatgc agttgcggat aaggcaagaa attgagaagg
     1441 aaaagaaaac agaaccttgg aaagaaaaat tctttgagag gttttatgga gaaaagctgg
     1501 gcatgtcaag agaggaatct gtgaagctca ctactggacc aaacaacgct ggagctcaaa
     1561 gtagttcttc atgtgggact tctggccttc cagtttctgc acagacagcc ttggcagaac
     1621 aacagccaaa aagcatgaaa agcccagctt ctccagagcc tggtttctgt gctactcttt
     1681 gccctatggt agaaattcca cctaaagata taatggcaga attggagtca gaggatatct
     1741 tgatccctga agaatctgta attcaggagg aaattgcaga agaggtagag actagtatct
     1801 gtgaatgcca ggatgaaaat cataagacaa tacctgaatt ttctgaggag gctgaaagtc
     1861 taaccaattc tcatgaagaa ccccaaatag cacctcctga agataacttg gaatcctgtg
     1921 ttatgatgaa tgatgtttta gaaactttgc ctcatattga agttaagata gaagggaagt
     1981 cagaatcacc ccaggaagaa atgacagttg ttatcgatca gttagaagtc tgtgactctc
     2041 ttattccttc cacttcatct atgactcatg tcagtgacac agaacataag gagtcagaaa
     2101 ctgcagtaga gaccagtacc cccaaaataa aaacagggtc atcttctcta gaaggccagt
     2161 ttccaaatga aggaattgct atagatatgg agctacagag tgaccctgaa gaacagcttt
     2221 cagaaaatgc ctgcatctct gaaacgtcct tttcttctga gagcccagag ggagcctgta
     2281 ccagcctgcc ttctccagga ggggaaacac agtccacatc agaagaatca tgtactccag
     2341 cctcccttga gacaacattt tgttctgagg tatctagcac tgaaaataca gacaaataca
     2401 accagagaaa ttccactgat gaaaactttc atgcatcttt gatgtcagaa atatctccaa
     2461 tatccacttc acctgaaata tcagaagcat ctcttatgtc caacttacca ttaacatctg
     2521 aagcatcacc agtatccaac ttacctttaa catcagaaac ctcaccgatg tctgacttac
     2581 ctttaacatc agaaacttct tcagtgtctt ccatgcttct cacctctgag accacttttg
     2641 tatccagttt gccacttcct tcagaaacat ctccaatttc caactcttcc ataaatgaga
     2701 gaatggcaca tcagcaaaga aagtcacctt ctgtatctga agagccactc tccccgcaga
     2761 aagatgagtc ttccgccact gccaaacctc tgggagagaa ccttacctcc cagcagaaga
     2821 atctgtctaa tactcccgaa cccatcataa tgagttcttc ttccattgct cctgaagcat
     2881 ttccgtctga agatttgcac aataagaccc tgagtcagca aacctgtaaa tcacatgttg
     2941 acactgagaa gccctaccct gcttcaattc cagaacttgc ttctactgaa atgataaaag
     3001 ttaaaaatca tagcgtcctg caaagaacag aaaaaaaagt gttaccttca ccattggaat
     3061 tatctgtctt ttctgaaggg acagataata agggaaatga gcttccatct gctaaattac
     3121 aggacaagca atatatctca tcagtggata aggctccatt ttcagaaggc tctagaaata
     3181 aaacacataa gcaagggagt acacagagtc ggttagaaac ctcacatact tccaagtcat
     3241 cagagccctc caagtcacct gatgggataa gaaatgaaag tagagattca gagatatcaa
     3301 agagaaaaac tgcagagcaa cacagctttg gaatctgtaa ggaaaagaga gctaggatag
     3361 aagatgatca gtcaacccgg aacatatcat ctagcagccc acctgagaaa gaacagcctc
     3421 ccagagagga accaagggtt ccccctctca agattcagct ttccaaaatt gggccacctt
     3481 ttataatcaa gagccaacca gtctccaaac ctgagtctcg agcatccact agcacatctg
     3541 tcagtggcgg gaggaacaca ggagccagga ccctcgcaga tatcaaggcc cgggcccaac
     3601 aagctcgggc ccagcgagag gctgctgcag ctgctgctgt ggctgctgca gcgagcattg
     3661 tctctggagc catgggaagt ccaggagagg gtggaaagac gagaactctg gcacacatca
     3721 aagagcagac aaaggctaag ctctttgcaa agcatcaagc tcgagcccat ctcttccaga
     3781 cctctaaaga gacccggttg cctcctccgc tcagctcaaa ggaagggcct ccaaacttag
     3841 aagtctcttc tacccctgaa acaaaaatgg aaggttcgac tggtgtcatt attgtcaatc
     3901 caaactgtag atctcctagc aacaagtctg cccacctccg ggagaccacc actgtactac
     3961 agcagtctct taacccaagt aaacttccag aaactgccac tgacttatct gtgcatagtt
     4021 ctgatgaaaa catacctgtg tcacatttat ctgagaaaat tgtttcatct acctcttctg
     4081 aaaatagcag tgtgcccatg ctttttaata aaaattctgt ccctgtatct gtttgcagca
     4141 ctgctatatc gggagcaatt aaagaacatc cctttgtgag ttctgttgat aaatcctctg
     4201 tcctaatgtc tgttgacagt gcaaacacta caatttctgc ttgtaatata agcatgttaa
     4261 aaaccatcca gggaactgac actccatgca tagccattat accaaaatgt attgaaagca
     4321 ctcccatttc agccactaca gagggctcca gcatatcaag ctccatggat gataagcagt
     4381 tactaatatc aagcagcagt gctagtaact tagtctccac tcagtacacc tctgtgccaa
     4441 ctccctccat cggaaacaat ttgccaaacc tctccactag ctctgtcttg attcccccaa
     4501 tgggaattaa caacagattt ccttctgaga agatagccat acctgggagt gaagaacagg
     4561 ccactgtatc catgggtacc actgtgagag cagccctcag ctgcagtgat tctgtagcgg
     4621 tcacagactc tctggttgca cacccgaccg tcgcaatgtt tactggaaac atgctgacaa
     4681 taaactctta tgatagtcct cccaagttaa gtgctgaaag cttggacaaa aattcagggc
     4741 ctcgaaacag ggcagataat tctggaaaac ctcagcaacc accagggggc tttgcaccag
     4801 cagccataaa ccgatcaatt ccgtgtaaag tcatcgttga ccacagcacc acgctgacct
     4861 ccagtttgtc tctgactgtc tccgttgaaa gctcagaagc cagcttggac ctgcagggca
     4921 gaccagtgag gacagaggca tccgtacagc ccgtggcgtg tcctcaggtg tctgtgatta
     4981 gcaggcctga gccagttgcc aacgaaggta tagatcacag ttccactttc attgctgctt
     5041 cggcagcaaa acaagacagt aaaacattgc cggccacctg cacaagtctc cgagaattac
     5101 cccttgttcc agataaatta aatgagccga ctgctcccag tcataacttt gctgagcagg
     5161 cacgtggccc agctcctttc aaaagtgaag cagacacaac ctgtagcaat cagtataacc
     5221 caagtaaccg gatttgctgg aatgatgatg ggatgaggag cacaggacag cctctggtta
     5281 ctcactcggg ttcaagtaaa caaaaagaat atctagagca aagctgtcca aaggctatca
     5341 aaactgaaca tgccaactac ttgaacgtgt cagaacttca tcccaggaat cttgtaacaa
     5401 atgttgctct tcctgtgaaa tctgaacttc acgaagcaga caagggcttt agaatggaca
     5461 ctgaagactt ccctggccct gagctgcctc ctccggctgc agagggagcc tctagtgtac
     5521 aacaaacaca gaacatgaaa gcttccacct caagtcccat ggaagaggct atttccttgg
     5581 ctaccgatgc cctgaagaga gtccctggtg cagggagctc aggctgtcgt ctgtcctctg
     5641 tggaggctaa caatccgctg gtgacgcagt tactacaggg caacctgcct ttggaaaaag
     5701 tgttgccaca gcccagattg ggagccaagc ttgaaatcaa caggcttcca ttgcctcttc
     5761 aaactacctc agtgggtaaa acagcaccag agagaaacgt tgaaattccg cccagctctc
     5821 caaatccaga tggtaagggc tacttggcag ggactctggc accactccaa atgagaaagc
     5881 gagaaaacca ccccaaaaag agagtagcta ggactgtagg agaacacact caagttaaat
     5941 gtgaaccagg aaaattgttg gtggagccag atgttaaagg ggtgccttgt gtcatcagtt
     6001 ccggcatcag tcagctagga cacagccagc catttaagca agaatggcta aacaagcact
     6061 ccatgcagaa cagaattgtt cacagccctg aggtcaaaca gcaaaagcgg ctgctcccct
     6121 cgtgtagctt ccagcagaac ctatttcatg ttgacaagaa tggcggcttc cacactgacg
     6181 ctggtacctc acacagacag cagttttacc aaatgcctgt ggctgccagg ggccccattc
     6241 ctactgcagc tctgttacag gcctcttcca agaccccagt ggggtgtaat gcatttgcct
     6301 tcaacaggca tcttgaacag aagggattgg gagaggttag tctttcctca gcacctcacc
     6361 agctaaggtt agccaacatg ttatccccca atatgcccat gaaagaaggt gatgaggtgg
     6421 gaggcactgc acacacaatg ccaaacaaag cactagtaca tccgccgccg ccaccgcctc
     6481 cccctccccc tccacccttg gctttgcccc cgcctccccc cccaccacct ccgctacctc
     6541 cacctctccc taatgcagaa gtcccatctg atcaaaaaca acctccagtt accatggaaa
     6601 ccactaagag acttagttgg ccacagtcca cgggcatatg tagcaatata aaatcggaac
     6661 ctctttcttt tgaggaaggt ttaagcagca gctgtgaact gggcatgaaa caagtttcct
     6721 atgaccagaa tgaaatgaaa gaacagttaa aagcattcgc gctaaaaagt gcagatttct
     6781 cttcctattt gctttctgag ccacaaaagc cttttaccca attagctgct cagaaaatgc
     6841 aggtgcagca acaacagcag ctctgtggaa attatccaac aatacacttt ggtagcacga
     6901 gtttcaaaag ggcagcatct gcaattgaaa agtccattgg gattttggga agtggctcca
     6961 atcctgccac aggcttgtct ggtcagaacg ctcagatgcc cgttcagaac tttgccgaca
     7021 gcagcaatgc agatgaattg gaactgaaat gctcttgccg gctgaaagcc atgattgtgt
     7081 gcaaaggctg tggggccttc tgccatgacg actgcatagg tccttcaaaa ctttgtgtag
     7141 catgcctggt tgtacgataa gagctgagtg aaagatgcag tatccctttt ccacacggaa
     7201 aagccaaata gcatcagcaa caacaaatag aataatgcag tggtttctat catgctaatt
     7261 tattttgctt tggagcaggt acctttcctc tatggcatta ttttcttgca tttctcataa
     7321 aggggaggat gcatcccaac tgaatggctc actggcatgt cttttatgtg ttcagttgcc
     7381 attcctagct ttggaaagtt tctatctgtc catgtgtata caagtcaatg ccccattttt
     7441 gtttttcttt taaccgaggt gtagatagga aaaggacatt tttaattact taataaccgg
     7501 aaatgcagat gtgtaaggag aatgagagga atgagttaaa gtggttatgc atttttctat
     7561 agatgagcca ttacagcaag gaattttaca gttgacttct ctgaacctag ctttaccaca
     7621 gtgattaaat cctatttaga aaggggaatc tgatttaaat gtgtgattcc ttgtatttgc
     7681 tcctatcaca aaagatatat taaaggaggt atgccattaa tgaaatccac tgtcctgagt
     7741 attatctttc ctcctgttgt actttctcag agactatggc agaatatctg gatcttcctt
     7801 ggattttgta cacatattgt agtgaaatgt gtcctacatc tgaaattgca tgggactcat
     7861 gcccagcaat ctggttctag gcctttgaca cctgataata tgagagcaat tggccagcca
     7921 atagccataa gcccagagga ttttagagct tcatgtgtgg cttttaagag caggtttgaa
     7981 aaaaaaaaaa aaaagaccct aaacttcaaa gcaaggaaat aagatgctgt atatgcacac
     8041 ataaatgcat tgataatgta aatatcctat tcacgctaat gaattactag atattaaaag
     8101 tggggggtgg aaatcattgt agccctttat tgatgacacc cattcattct ggcttcagac
     8161 cttgcagagg ttggccatat ctttttaaaa gtgtcattga tgataaaagg gactacccaa
     8221 ggctaaagcg tcaacatttt tcagaacaat tgcttttctc attatttaca acctatataa
     8281 ttcaactttg aaataaccct tattttgaaa aaaactgttc taggtatggc atgttttgtt
     8341 ttttccctca ggtatattca atacattctt catatttggg ggaactgctt ttgaaaaagg
     8401 gatactgcat cttaacagtt atcttcagtg tgcatccagt aaagtctgcg tgttgagtat
     8461 ttgagctaca ttccatatat tttggccacc ctcataccag aggacagccg tagttcagga
     8521 ggctctgttt tcaaaagcaa gttcaaaagc acatgcacat tgagggaaga tgaaagaaaa
     8581 caaaaaccaa ccatgcccat ttaacttcag attacagagc acaaaatgtg gagtgccatt
     8641 aacccattga ttgattgtgg ctgtagaatt taaaaagcaa acatgggggg ggtggggaat
     8701 catctgtatc cagggtaaga atgaccaata ttttcacatg ggatcatcct aatcactgta
     8761 aactttttga cattcccgta aaacacgtct taaaagttga ctggaatgag cagaaattac
     8821 attcctgaaa acaagaacac ttgtgatttt tttataataa ccaactatat aatatgtttt
     8881 tttctccgta tttattgtga tgttgccaaa tttctttctt taggtgatag agacatcatt
     8941 tcgaataaca aattgctgat agtgagaggt actttacttt atcttagaaa gaaagaaaag
     9001 gcctgctgct gtagccatga ttttggtgct tcagtactgt catttctgtt tttcacagta
     9061 aaattggcaa aactaatgac tgagaaagtt agttagcaat gcacttaacc agccttaccc
     9121 gaggaaagca gcataagttc cagtggatcc ttagttttct cttcactgcc tgccaggttg
     9181 tgttggggtg gcatccttat cagtggtgtc tttgtctgcc tggctctgat atcatcctga
     9241 tttcgaagat catgctgtta gtctcacagc ggggcacccc tataatatag acacttgcat
     9301 gccaactcct tccaaattca ttcactgcta taccagtgtc agatactgtc tacaccagca
     9361 aattgaaaac catcacatca gtaactgaca tggtgtcgga agaaactcca gtctacagaa
     9421 cctttgcact cagtatgtga ggtacaattg aggctgatga gatactgagc tcattatcag
     9481 ttccttcatt gttgaatact gcctgcaagt tgcttattgg gaataagaaa aagtgtcctt
     9541 ctgtatgttt tgatttttca cataacaggc ttggagaatc agttgttgat agtgcatgga
     9601 ttctatcatc caggtccgat tagcataatt tttctgcgcc cttttttttt ttttttttgc
     9661 tccagttcaa attcctagtg ttctcatatg gtttatatta ctgaaaaatt aatatcttta
     9721 agctaaaata taagggaaag gaagaagaga aatttagatg taaaatgtgg gtcagatatt
     9781 ttataggttt ccatttaata tatatataca cacaaaatcc agtatattct gagtaggttc
     9841 ttagttttct gatttctaca tgtgtctgct atgtgcaaat gtagtatatt atttactaca
     9901 tggttattgt ggtgtagtgt gcaaatgtta gcatgtgcat tatcctgatt actggacaca
     9961 gttttattct tatcaggaat agaacttgca catgtaccat acaatttttt tttttttaat
    10021 cacaaaggat gtatttccct aagtagcact tttggagcag gggaaggaag aaaactgcta
    10081 ttccctaatc ctcctcctat aagatatgca tatgcctgag gtgtataaag acatttaggt
    10141 tagttaatgg gcatgttaat aagagcattg tgatgcaaat tcattttcaa aatgaaaata
    10201 attttttctt ccccaaaatg agaatagtag aaattttggt gaactgagca ccatagttat
    10261 ataatatttc cctgcgaagg tctagtttga agaaaaaaaa aacttgaaaa caaacctatg
    10321 aggttgagcc tggttgctat aaagactttg ggatggtatg tgtttctggg atgagctata
    10381 tagaaagttt agttgactgc tggaccttgg atattcttac tcggttcaaa atacacaaaa
    10441 acaagctggt acatatggtg ctttataatg cagtcgagag taagaataca tgttgctgta
    10501 gccccatttc ctttacaaac ccccgcacaa tttcaaaagt ggaagttacc gtggcctctg
    10561 gtttcccagt tcctccgtgg tgtaatgaag tcccaggttt tagaagctga agccaagcct
    10621 agggtagcat cgtctgtacg gtggtgaggc ccagcacagc ggtagctgcc tagtgggaat
    10681 gaccccctga gcatgcagag cccagagtca ctgtccatct gcgttcccag agaacaattg
    10741 tgaagagtcc tagaacagga actctgaatc agactgtcag gggcctatgt agccaaaatg
    10801 ttactaaata taaaattcct gtcgtcttca gtcttcatgc tttgtcactg taaaaagaac
    10861 aaaaagcatt tttactataa tttaaaggag ctgctttttt atagcacata ctatttcgct
    10921 ttgtactata tttgatagtt gcagaataat ttagactgta gaaaaacttt gttgtatttg
    10981 tactgttctt gaatgtttca aagaaagtgc tttacttggt tgccataatt ttcttgtact
    11041 gctgtattcc cctccttccc ctgcttcatg gaaacctgat atgatacata ttttcagtag
    11101 tttttttttt ttaatgtgtg ttcgtttgta caattttttt tttttggtca gtattctgaa
    11161 tgtttacatc tgtttgacat tagccattta atgccttttt taaaggcggt attactccta
    11221 cagtgtaaca gcaaaatgtg aaatcaaccc aaacataaca tgcatgacaa acacagattg
    11281 ggggcgaagg ccctgaacct acatagacat tttatatcag catacagaaa agtaaaatcc
    11341 tccttcagtc ctctaccaaa gaatatatta ttcccacagg aaaaactcag aaaaggtgtg
    11401 taaaatcctc agaaggggga gcagttgatt cagtaagact gcgacaattt aatactgtta
    11461 cgcttgcttt gatacctgac taaatgtgac tgagtgcaac aagcatttaa gaaaattttt
    11521 agacagtgtt ttgtttagaa ttcagggatc atgcattctt taatggtgct gtttgttttt
    11581 tatttctttt ctacaaagaa aacaagtgtt gcctacaaaa gtgactgctc acaataccat
    11641 aagttaaatg aaatgaaatg tttgtctctt catgtttctc tgtctttccc tttctcaaag
    11701 taacaacttg ggtttcaata ttaagatatt ctggagaaaa taaagaaatt aaacatgaaa
//
